Insecticide and mildew-proof tobacco alcoholization composition and preparation method and application thereof

Abstract

The present invention relates to an insecticidal and mildew-proof tobacco alcoholization composition and a preparation method and application thereof, and belongs to the technical field of tobacco processing. The present invention provides an insecticidal and mildew-proof tobacco alcoholization composition, the components of which include glucose oxidase, catalase, glucose, mildew inhibitor, chitosan, glycerol, acetic acid and water. The tobacco alcoholization composition provided by the present invention is composed of natural ingredients, is safe and non-toxic to use, and is sprayed on tobacco leaves to form a thin film with mechanical properties and barrier properties, and has a moisturizing effect, which can maintain the moisture content of tobacco leaves during the alcoholization process, and is more conducive to the natural alcoholization of tobacco leaves. The glucose oxidase and catalase in the film can oxidize the glucose contained in the film liquid to consume oxygen, and when interacting with the mildew inhibitor, the effect of insect resistance and mildew prevention can be achieved on naturally alcoholized tobacco leaves by controlling the oxygen content and inhibiting the growth of mold.

Description

Technical Field

[0001] The invention relates to an insecticidal and mildew-proof tobacco alcoholization composition and a preparation method and application thereof, belonging to the technical field of tobacco processing. Background Art

[0002] Before tobacco leaves enter the cigarette production process, they need to be stored for a period of time for natural aging after the initial baking and re-baking of the leaves, usually 1 to 3 years. During the natural aging process, the tobacco leaves will undergo a series of chemical changes. In particular, under the synergistic effect of microorganisms, the green smell and irritation of the tobacco leaves will be reduced, the fragrance will increase, the taste will be mellower, and the color will be more uniform. The natural aging of tobacco leaves can improve the aroma and taste quality of the tobacco leaves. However, tobacco leaves are rich in nutrients such as sugars, nicotine, and protein. They are prone to insects when in close contact with the external environment for a long time. In addition, when the temperature is too high and the humidity is too high during the natural aging process, the tobacco leaves are also prone to mold. Infested or moldy tobacco leaves not only seriously affect the quality of the tobacco leaves themselves, causing losses to cigarette manufacturers and national tax revenue, but the toxins produced by the mold also directly endanger the health of consumers.

[0003] In order to solve the problem of tobacco leaf pests, in the past, phosphine gas fumigation was usually used for comprehensive prevention and control, or a sealed ultra-low oxygen storage method was used to control the oxygen in the storage environment to below 2% to quickly inhibit and kill microorganisms and plant pests in the storage environment. Although phosphine fumigation is effective, phosphine is highly toxic, flammable and explosive. It is easy to cause human poisoning and other serious safety accidents during the fumigation process, and it will also cause certain harm to the surrounding environment and humans and animals. In addition, it is also troublesome to carry out non-toxic treatment of waste gas, waste liquid and waste residue after fumigation, which is currently prohibited. The sealed ultra-low oxygen storage method is used to reduce oxygen, kill insects and prevent mildew, which is achieved through natural oxygen reduction, microbial oxygen reduction, and chemical oxygen reduction. Chemical oxygen reduction or physical controlled atmosphere oxygen reduction methods can reduce the oxygen concentration in the tobacco storage environment to below 10% and maintain it for a period of time to kill insects; natural oxygen reduction and microbial oxygen reduction have poor effects and slow oxygen reduction rates; chemical oxygen reduction agents such as iron reducing agents reduce oxygen too quickly and can quickly reduce the oxygen concentration to below 2%. Although this can quickly kill insects, tobacco leaves are in a low-oxygen environment below 2% for a long time, which excessively reduces the aging rate of tobacco leaves, and the tobacco leaves are prone to darkening in color, reducing their usability; physical controlled atmosphere oxygen reduction, such as oxygen reduction by introducing nitrogen, ozone, carbon dioxide and other gases for replacement, will require investment in gas production equipment, increase the company's production costs, and also result in unchanged operations and the risk of gas leakage.

[0004] In the past, in order to solve the problem of tobacco leaf mildew, the temperature and humidity of the warehouse were usually adjusted by installing temperature and humidity control equipment to control mildew, or natural ventilation or calcium chloride dehumidification was used to adjust the temperature and humidity of the warehouse, thereby controlling the tobacco leaves from absorbing moisture and mildew. Installing temperature and humidity control equipment to adjust the temperature and humidity of the warehouse, or natural ventilation or calcium chloride dehumidification were used to adjust the temperature and humidity of the warehouse to control the tobacco leaves from absorbing moisture and mildew. This cannot fundamentally solve the problem of tobacco leaf mildew, because mold is not only distributed on tobacco leaves, but mold spores are widely distributed in the air. When conditions are right, mold spores will germinate, causing tobacco leaves to become mildewed.

[0005] How to effectively control tobacco leaf insect infestation and mildew during the natural aging period of tobacco leaves on the basis of low cost and energy saving and consumption reduction is the core problem to be solved by the present invention. Summary of the invention

[0006] To solve the above problems, the present invention provides an insecticidal and mildew-proof tobacco alcoholization composition, the ingredients of which include glucose oxidase, catalase, glucose, mildew inhibitor, chitosan, glycerol, acetic acid and water.

[0007] In one embodiment of the present invention, the ingredients of the tobacco alcoholization composition include, by weight, 0.0016 to 0.0065 parts of glucose oxidase, 0.0016 to 0.0065 parts of catalase, 0.033 to 0.33 parts of glucose, 0.0016 to 0.0065 parts of mildew inhibitors, 0.882 parts of chitosan, 0.156 parts of glycerol, 0.0873 parts of acetic acid and 98.5252 to 98.8369 parts of water.

[0008] In one embodiment of the present invention, the antifungal agent comprises one or a combination of more than one of dimethyl fumarate, sodium diacetate and calcium propionate.

[0009] In one embodiment of the present invention, the glucose oxidase is food grade glucose oxidase, and the enzyme activity is ≥10000 U / g.

[0010] In one embodiment of the present invention, the catalase is food grade catalase, and the enzyme activity is ≥100000 U / g.

[0011] In one embodiment of the present invention, the chitosan is industrial grade chitosan.

[0012] In one embodiment of the present invention, the deacetylation of the chitosan is ≥ 95%.

[0013] In one embodiment of the present invention, the glycerol is food grade glycerol.

[0014] In one embodiment of the present invention, the purity of the acetic acid is ≥98%.

[0015] The present invention also provides a method for preparing the above-mentioned insecticidal and mildew-proof tobacco alcoholization composition, which comprises adding a glycerol aqueous solution to a mixed aqueous solution of glucose oxidase, catalase, glucose, acetic acid, a mildew inhibitor and chitosan, and mixing to obtain the insecticidal and mildew-proof tobacco alcoholization composition.

[0016] In one embodiment of the present invention, the method comprises the following steps:

[0017] Step 1: adding glucose oxidase and catalase into water, and mixing to obtain a mixture A;

[0018] Step 2: adding glucose into water and mixing to obtain mixture B;

[0019] Step 3: adding acetic acid to water and mixing to obtain a mixture C;

[0020] Step 4: adding glycerol to water and mixing to obtain a mixture D;

[0021] Step 5: Add the mildew inhibitor into water and mix to obtain a mixture E;

[0022] Step 6: adding chitosan to mixture C, and mixing to obtain mixture F;

[0023] Step 7: Mixing mixture A, mixture B and mixture E to obtain mixture G;

[0024] Step 8: Add mixture G to mixture F, and mix to obtain mixture H;

[0025] Step nine: Add mixture D into mixture H, and mix to obtain an insecticide- and mildew-proof tobacco alcoholization composition.

[0026] In one embodiment of the present invention, the step 1 is: adding 0.05-0.2 g of glucose oxidase and 0.05-0.2 g of catalase into 100 mL of water, and mixing to obtain a mixture A.

[0027] In one embodiment of the present invention, the step 1 further comprises sterile filtering using a filter membrane with a pore size of 0.22 μm after mixing the glucose oxidase, catalase and water.

[0028] In one embodiment of the present invention, the step 2 is: adding 1-10 g of glucose into 90-99 mL of water, and mixing to obtain a mixture B.

[0029] In one embodiment of the present invention, the step 2 further comprises sterile filtering using a filter membrane with a pore size of 0.22 μm after mixing the glucose and water.

[0030] In one embodiment of the present invention, the step three is: adding 1 mL of acetic acid into 99 mL of water, and mixing to obtain a mixture C.

[0031] In one embodiment of the present invention, the step four is: adding 6 mL of glycerol into 94 mL of water, and mixing to obtain a mixture D.

[0032] In one embodiment of the present invention, the step five is: adding 0.05-0.2 g of the mildew inhibitor into 100 mL of water, and mixing to obtain a mixture E.

[0033] In one embodiment of the present invention, the step six is: adding 1 g of chitosan into 99 mL of mixture C, and mixing to obtain mixture F.

[0034] In one embodiment of the present invention, the step seven is: mixing mixture A, mixture B and mixture E in a volume ratio of 1:1:1 to obtain mixture G.

[0035] In one embodiment of the present invention, the step eight is: adding 10 mL of mixture G into 90 mL of mixture F, and mixing to obtain mixture H.

[0036] In one embodiment of the present invention, the step nine is: adding the mixture D into the mixture H at a rate of 1-2 mL / min, and mixing to obtain the insecticide and mildew-proof tobacco alcoholization composition.

[0037] In one embodiment of the present invention, the rate in step nine is 2 mL / min.

[0038] The present invention also provides the use of the above insecticide and mildew-proof tobacco alcoholization composition or the above method for preparing the above insecticide and mildew-proof tobacco alcoholization composition in tobacco alcoholization.

[0039] The technical solution of the present invention has the following advantages:

[0040] 1. The present invention provides an insecticidal and mildew-proof tobacco alcoholization composition, the ingredients of which include glucose oxidase, catalase, glucose, mildew inhibitor, chitosan, glycerol, acetic acid and water. The tobacco alcoholization composition adopts bioenzymatic oxygen reduction technology, and glucose oxidase and catalase form an oxidoreductase system; wherein, glucose oxidase can oxidize glucose to generate D-gluconolactone in the presence of molecular oxygen, and consume oxygen to generate hydrogen peroxide at the same time, and catalase can decompose hydrogen peroxide to generate water and 1 / 2 oxygen to consume oxygen; the bioenzymatic deoxygenation method is fast and effective, and has outstanding advantages in energy saving, emission reduction and efficiency improvement, and can control oxygen within a certain concentration range (6-12%) without affecting the natural alcoholization of tobacco leaves, and has the advantages of simple operation and environmental friendliness.

[0041] 2. In the tobacco alcoholization composition of the present invention, the optional mildew inhibitors include dimethyl fumarate, sodium diacetate and calcium propionate; sodium diacetate and calcium propionate mildew inhibitors are new food and feed mildew inhibitors with stable properties and low prices, and have good inhibitory ability against molds; dimethyl fumarate is widely used in food, grain, feed, tobacco, leather and clothing for anti-corrosion, mildew prevention and preservation, and has the characteristics of low toxicity, high efficiency and broad-spectrum antibacterial, has a special antibacterial effect on molds, has insecticidal activity, and also has contact killing and fumigation effects.

[0042] 3. During the natural aging process of tobacco leaves, especially when the natural aging time is long, the moisturizing ability of tobacco leaves decreases, the leaves become brittle, and are easily broken in subsequent processing; therefore, it is particularly important to maintain appropriate moisture content of tobacco leaves during the aging process of tobacco leaves; the chitosan in the tobacco aging composition of the present invention is a product of chitin deacetylation, a linear polymer formed by connecting 2-acetamido-2-deoxy-D-pyranose glucose through β-D-(1-4) glycosidic bonds, and has the advantages of good biocompatibility, degradability, regeneration, antibacterial and antiseptic properties, and film-forming properties; due to the film-forming property of chitosan, a layer of chitosan film will be formed after being sprayed on tobacco leaves, which has certain mechanical properties and barrier properties, has a moisturizing effect, can well maintain the moisture content of tobacco leaves during the aging process, and is more conducive to the natural aging of tobacco leaves; at the same time, it also has a barrier effect on mold spores and oxygen in the air, and has an insect-resistant and mildew-proof effect.

[0043] 4. The present invention can effectively avoid the phenomenon that the low oxygen sealing time is too long, the tobacco aging speed is excessively reduced, and the availability of tobacco leaves is reduced.

[0044] 5. The raw materials used in the present invention are easily available, the process is simple, the effect is significant, and the cost is low, so it is very easy to promote. In addition, the raw materials used in the present invention are highly safe and low in toxicity. No toxic or harmful substances will be generated during the insecticide and mildew prevention process of stored tobacco leaves. Therefore, the tobacco leaves will not be contaminated during the insecticide process, and no environmental pollution will be caused, which meets the requirements of clean production. DETAILED DESCRIPTION

[0045] The following examples are provided for a better understanding of the present invention, but are not intended to limit the best mode of implementation, nor to limit the content and protection scope of the present invention. Any product identical or similar to the present invention obtained by anyone under the inspiration of the present invention or by combining the features of the present invention with other prior arts shall fall within the protection scope of the present invention.

[0046] If no specific experimental steps or conditions are specified in the following examples, the conventional experimental steps or conditions described in the literature in the field can be used. If no manufacturer is specified for the reagents or instruments used, they are all conventional reagent products that can be purchased commercially.

[0047] The glucose oxidase involved in the following embodiments was purchased from Zhengzhou Best Food Additive Co., Ltd., with an enzyme activity of ≥10000U / g; catalase was purchased from Zhengzhou Best Food Additive Co., Ltd., with an enzyme activity of ≥100000U / g; glucose was purchased from Henan Huiteng Chemical Products Sales Co., Ltd., food grade, purity ≥99%; dimethyl fumarate was purchased from Shanghai McLean Biochemical Technology Co., Ltd., purity ≥99%; sodium diacetate was purchased from Henan Chengxiang Food Additive Co., Ltd., food grade, purity ≥99%; calcium propionate was purchased from Zhengzhou Best Food Additive Co., Ltd., food grade, purity ≥99%; chitosan was purchased from Zhengzhou Best Food Additive Co., Ltd., purity ≥99%, deacetylation ≥95%; glycerol was purchased from Henan Youchuang Chemical Products Co., Ltd., food grade, content ≥99%; acetic acid was purchased from Henan Jieneng Chemical Products Co., Ltd., food grade, content ≥98%.

[0048] Example 1-1: An insecticidal and mildew-proof tobacco alcoholization composition and its preparation method

[0049] This embodiment provides an insecticidal and mildew-proof tobacco leaf alcoholization composition, the ingredients of which include glucose oxidase, catalase, glucose, mildew inhibitors (dimethyl fumarate, sodium diacetate and calcium propionate), chitosan, glycerol and acetic acid.

[0050] The preparation method of the insecticide and mildew-proof tobacco alcoholization composition comprises the following steps:

[0051] Step 1: Add 0.05 g of glucose oxidase and 0.05 g of catalase into 100 mL of distilled water, mix for 2 min under magnetic stirring at a speed of 150 rpm, and sterile filter using a filter membrane with a pore size of 0.22 μm to obtain a mixture A;

[0052] Step 2: Add 1 g of glucose into 99 mL of distilled water, mix for 2 min under magnetic stirring at a speed of 150 rpm, and sterile filter using a 0.22 μm pore size filter membrane to obtain a mixture B;

[0053] Step 3: Add 1 mL of acetic acid into 99 mL of distilled water, and mix for 2 min under magnetic stirring at a speed of 150 rpm to obtain a mixture C;

[0054] Step 4: Add 6 mL of glycerol into 94 mL of distilled water and mix for 2 min under magnetic stirring at a speed of 150 rpm to obtain a mixture D;

[0055] Step 5: Add 0.05 g of dimethyl fumarate, 0.05 g of sodium diacetate and 0.05 g of calcium propionate into 100 mL of distilled water, and mix for 2 min under magnetic stirring at a speed of 150 rpm to obtain a mixture E;

[0056] Step 6: Add 1 g of chitosan to 99 mL of mixture C, and mix for 30 min under magnetic stirring at a speed of 150 rpm to obtain mixture F;

[0057] Step 7: Mixture A, mixture B and mixture E in a volume ratio of 1:1:1, and mix under magnetic stirring at a rotation speed of 150 rpm for 5 minutes to obtain mixture G;

[0058] Step 8: Add 10 mL of mixture G to 90 mL of mixture F, and mix for 30 min under magnetic stirring at a speed of 150 rpm to obtain mixture H;

[0059] Step nine: Add 2 mL of mixture D into 98 mL of mixture H at a rate of 2 mL / min, and mix for 30 min under magnetic stirring at a rotation speed of 150 rpm to obtain an insecticide and mildew-proof tobacco alcoholization composition.

[0060] Example 1-2: An insecticidal and mildew-proof tobacco alcoholization composition and its preparation method

[0061] This embodiment provides an insecticidal and mildew-proof tobacco leaf alcoholization composition, the ingredients of which include glucose oxidase, catalase, glucose, mildew inhibitors (dimethyl fumarate, sodium diacetate and calcium propionate), chitosan, glycerol and acetic acid.

[0062] The preparation method of the insecticide and mildew-proof tobacco alcoholization composition comprises the following steps:

[0063] Step 1: Add 0.1 g of glucose oxidase and 0.1 g of catalase into 100 mL of distilled water, mix for 2 min under magnetic stirring at a speed of 150 rpm, and sterile filter using a filter membrane with a pore size of 0.22 μm to obtain a mixture A;

[0064] Step 2: Add 3 g of glucose into 97 mL of distilled water, mix for 2 min under magnetic stirring at a speed of 150 rpm, and sterile filter using a 0.22 μm pore size filter membrane to obtain a mixture B;

[0065] Step 3: Add 1 mL of acetic acid into 99 mL of distilled water, and mix for 2 min under magnetic stirring at a speed of 150 rpm to obtain a mixture C;

[0066] Step 4: Add 6 mL of glycerol into 94 mL of distilled water and mix for 2 min under magnetic stirring at a speed of 150 rpm to obtain a mixture D;

[0067] Step 5: Add 0.05 g of dimethyl fumarate, 0.05 g of sodium diacetate and 0.05 g of calcium propionate into 100 mL of distilled water, and mix for 2 min under magnetic stirring at a speed of 150 rpm to obtain a mixture E;

[0068] Step 6: Add 1 g of chitosan to 99 mL of mixture C, and mix for 30 min under magnetic stirring at a speed of 150 rpm to obtain mixture F;

[0069] Step 7: Mixture A, mixture B and mixture E in a volume ratio of 1:1:1, and mix under magnetic stirring at a rotation speed of 150 rpm for 5 minutes to obtain mixture G;

[0070] Step 8: Add 10 mL of mixture G to 90 mL of mixture F, and mix for 30 min under magnetic stirring at a speed of 150 rpm to obtain mixture H;

[0071] Step nine: Add 2 mL of mixture D into 98 mL of mixture H at a rate of 2 mL / min, and mix for 30 min under magnetic stirring at a rotation speed of 150 rpm to obtain an insecticide and mildew-proof tobacco alcoholization composition.

[0072] Example 1-3: An insecticidal and mildew-proof tobacco alcoholization composition and its preparation method

[0073] This embodiment provides an insecticidal and mildew-proof tobacco leaf alcoholization composition, the ingredients of which include glucose oxidase, catalase, glucose, mildew inhibitors (dimethyl fumarate, sodium diacetate and calcium propionate), chitosan, glycerol and acetic acid.

[0074] The preparation method of the insecticide and mildew-proof tobacco alcoholization composition comprises the following steps:

[0075] Step 1: Add 0.15 g of glucose oxidase and 0.15 g of catalase into 100 mL of distilled water, mix for 2 min under magnetic stirring at a speed of 150 rpm, and sterile filter using a filter membrane with a pore size of 0.22 μm to obtain a mixture A;

[0076] Step 2: Add 6 g of glucose into 94 mL of distilled water, mix for 2 min under magnetic stirring at a speed of 150 rpm, and sterile filter using a 0.22 μm pore size filter membrane to obtain a mixture B;

[0077] Step 3: Add 1 mL of acetic acid into 99 mL of distilled water, and mix for 2 min under magnetic stirring at a speed of 150 rpm to obtain a mixture C;

[0078] Step 4: Add 6 mL of glycerol into 94 mL of distilled water and mix for 2 min under magnetic stirring at a speed of 150 rpm to obtain a mixture D;

[0079] Step 5: Add 0.05 g of dimethyl fumarate, 0.05 g of sodium diacetate and 0.05 g of calcium propionate into 100 mL of distilled water, and mix for 2 min under magnetic stirring at a speed of 150 rpm to obtain a mixture E;

[0080] Step 6: Add 1 g of chitosan to 99 mL of mixture C, and mix for 30 min under magnetic stirring at a speed of 150 rpm to obtain mixture F;

[0081] Step 7: Mixture A, mixture B and mixture E in a volume ratio of 1:1:1, and mix under magnetic stirring at a rotation speed of 150 rpm for 5 minutes to obtain mixture G;

[0082] Step 8: Add 10 mL of mixture G to 90 mL of mixture F, and mix for 30 min under magnetic stirring at a speed of 150 rpm to obtain mixture H;

[0083] Step nine: Add 2 mL of mixture D into 98 mL of mixture H at a rate of 2 mL / min, and mix for 30 min under magnetic stirring at a rotation speed of 150 rpm to obtain an insecticide and mildew-proof tobacco alcoholization composition.

[0084] Example 1-4: An insecticidal and mildew-proof tobacco alcoholization composition and its preparation method

[0085] This embodiment provides an insecticidal and mildew-proof tobacco leaf alcoholization composition, the ingredients of which include glucose oxidase, catalase, glucose, mildew inhibitors (dimethyl fumarate, sodium diacetate and calcium propionate), chitosan, glycerol and acetic acid.

[0086] The preparation method of the insecticide and mildew-proof tobacco alcoholization composition comprises the following steps:

[0087] Step 1: Add 0.2 g of glucose oxidase and 0.2 g of catalase into 100 mL of distilled water, mix for 2 min under magnetic stirring at a speed of 150 rpm, and sterile filter using a filter membrane with a pore size of 0.22 μm to obtain a mixture A;

[0088] Step 2: Add 10 g of glucose into 90 mL of distilled water, mix for 2 min under magnetic stirring at a speed of 150 rpm, and sterile filter using a 0.22 μm pore size filter membrane to obtain a mixture B;

[0089] Step 3: Add 1 mL of acetic acid into 99 mL of distilled water, and mix for 2 min under magnetic stirring at a speed of 150 rpm to obtain a mixture C;

[0090] Step 4: Add 6 mL of glycerol into 94 mL of distilled water and mix for 2 min under magnetic stirring at a speed of 150 rpm to obtain a mixture D;

[0091] Step 5: Add 0.05 g of dimethyl fumarate, 0.05 g of sodium diacetate and 0.05 g of calcium propionate into 100 mL of distilled water, and mix for 2 min under magnetic stirring at a speed of 150 rpm to obtain a mixture E;

[0092] Step 6: Add 1 g of chitosan to 99 mL of mixture C, and mix for 30 min under magnetic stirring at a speed of 150 rpm to obtain mixture F;

[0093] Step 7: Mixture A, mixture B and mixture E in a volume ratio of 1:1:1, and mix under magnetic stirring at a rotation speed of 150 rpm for 5 minutes to obtain mixture G;

[0094] Step 8: Add 10 mL of mixture G to 90 mL of mixture F, and mix for 30 min under magnetic stirring at a speed of 150 rpm to obtain mixture H;

[0095] Step nine: Add 2 mL of mixture D to 98 mL of mixture H at a rate of 2 mL / min, and mix for 30 min under magnetic stirring at a rotation speed of 150 rpm to obtain an insecticide and mildew-proof tobacco alcoholization composition.

[0096] Example 1-5: An insecticidal and mildew-proof tobacco alcoholization composition and its preparation method

[0097] This embodiment provides an insecticidal and mildew-proof tobacco leaf alcoholization composition, the ingredients of which include glucose oxidase, catalase, glucose, mildew inhibitor (sodium diacetate), chitosan, glycerol and acetic acid.

[0098] The preparation method of the insecticide and mildew-proof tobacco alcoholization composition comprises the following steps:

[0099] Step 1: Add 0.05 g of glucose oxidase and 0.05 g of catalase into 100 mL of distilled water, mix for 2 min under magnetic stirring at a speed of 150 rpm, and sterile filter using a filter membrane with a pore size of 0.22 μm to obtain a mixture A;

[0100] Step 2: Add 1 g of glucose into 99 mL of distilled water, mix for 2 min under magnetic stirring at a speed of 150 rpm, and sterile filter using a 0.22 μm pore size filter membrane to obtain a mixture B;

[0101] Step 3: Add 1 mL of acetic acid into 99 mL of distilled water, and mix for 2 min under magnetic stirring at a speed of 150 rpm to obtain a mixture C;

[0102] Step 4: Add 6 mL of glycerol into 94 mL of distilled water and mix for 2 min under magnetic stirring at a speed of 150 rpm to obtain a mixture D;

[0103] Step 5: Add 0.2 g of sodium diacetate into 100 mL of distilled water, and mix for 2 min under magnetic stirring at a speed of 150 rpm to obtain a mixture E;

[0104] Step 6: Add 1 g of chitosan to 99 mL of mixture C, and mix for 30 min under magnetic stirring at a speed of 150 rpm to obtain mixture F;

[0105] Step 7: Mixture A, mixture B and mixture E in a volume ratio of 1:1:1, and mix under magnetic stirring at a rotation speed of 150 rpm for 5 minutes to obtain mixture G;

[0106] Step 8: Add 10 mL of mixture G to 90 mL of mixture F, and mix for 30 min under magnetic stirring at a speed of 150 rpm to obtain mixture H;

[0107] Step nine: Add 2 mL of mixture D into 98 mL of mixture H at a rate of 2 mL / min, and mix for 30 min under magnetic stirring at a rotation speed of 150 rpm to obtain an insecticide and mildew-proof tobacco alcoholization composition.

[0108] Example 1-6: An insecticidal and mildew-proof tobacco alcoholization composition and its preparation method

[0109] This embodiment provides an insecticidal and mildew-proof tobacco leaf alcoholization composition, the ingredients of which include glucose oxidase, catalase, glucose, mildew inhibitor (sodium diacetate), chitosan, glycerol and acetic acid.

[0110] The preparation method of the insecticide and mildew-proof tobacco alcoholization composition comprises the following steps:

[0111] Step 1: Add 0.1 g of glucose oxidase and 0.1 g of catalase into 100 mL of distilled water, mix for 2 min under magnetic stirring at a speed of 150 rpm, and sterile filter using a filter membrane with a pore size of 0.22 μm to obtain a mixture A;

[0112] Step 2: Add 3 g of glucose into 97 mL of distilled water, mix for 2 min under magnetic stirring at a speed of 150 rpm, and sterile filter using a 0.22 μm pore size filter membrane to obtain a mixture B;

[0113] Step 3: Add 1 mL of acetic acid into 99 mL of distilled water, and mix for 2 min under magnetic stirring at a speed of 150 rpm to obtain a mixture C;

[0114] Step 4: Add 6 mL of glycerol into 94 mL of distilled water and mix for 2 min under magnetic stirring at a speed of 150 rpm to obtain a mixture D;

[0115] Step 5: Add 0.2 g of sodium diacetate into 100 mL of distilled water, and mix for 2 min under magnetic stirring at a speed of 150 rpm to obtain a mixture E;

[0116] Step 6: Add 1 g of chitosan to 99 mL of mixture C, and mix for 30 min under magnetic stirring at a speed of 150 rpm to obtain mixture F;

[0117] Step 7: Mixture A, mixture B and mixture E in a volume ratio of 1:1:1, and mix under magnetic stirring at a rotation speed of 150 rpm for 5 minutes to obtain mixture G;

[0118] Step 8: Add 10 mL of mixture G to 90 mL of mixture F, and mix for 30 min under magnetic stirring at a speed of 150 rpm to obtain mixture H;

[0119] Step nine: Add 2 mL of mixture D into 98 mL of mixture H at a rate of 2 mL / min, and mix for 30 min under magnetic stirring at a rotation speed of 150 rpm to obtain an insecticide and mildew-proof tobacco alcoholization composition.

[0120] Example 1-7: An insecticidal and mildew-proof tobacco alcoholization composition and its preparation method

[0121] This embodiment provides an insecticidal and mildew-proof tobacco leaf alcoholization composition, the ingredients of which include glucose oxidase, catalase, glucose, mildew inhibitor (sodium diacetate), chitosan, glycerol and acetic acid.

[0122] The preparation method of the insecticide and mildew-proof tobacco alcoholization composition comprises the following steps:

[0123] Step 1: Add 0.15 g of glucose oxidase and 0.15 g of catalase into 100 mL of distilled water, mix for 2 min under magnetic stirring at a speed of 150 rpm, and sterile filter using a filter membrane with a pore size of 0.22 μm to obtain a mixture A;

[0124] Step 2: Add 6 g of glucose into 94 mL of distilled water, mix for 2 min under magnetic stirring at a speed of 150 rpm, and sterile filter using a 0.22 μm pore size filter membrane to obtain a mixture B;

[0125] Step 3: Add 1 mL of acetic acid into 99 mL of distilled water, and mix for 2 min under magnetic stirring at a speed of 150 rpm to obtain a mixture C;

[0126] Step 4: Add 6 mL of glycerol into 94 mL of distilled water and mix for 2 min under magnetic stirring at a speed of 150 rpm to obtain a mixture D;

[0127] Step 5: Add 0.2 g of sodium diacetate into 100 mL of distilled water, and mix for 2 min under magnetic stirring at a speed of 150 rpm to obtain a mixture E;

[0128] Step 6: Add 1 g of chitosan to 99 mL of mixture C, and mix for 30 min under magnetic stirring at a speed of 150 rpm to obtain mixture F;

[0129] Step 7: Mixture A, mixture B and mixture E in a volume ratio of 1:1:1, and mix under magnetic stirring at 150 rpm for 5 minutes to obtain mixture G;

[0130] Step 8: Add 10 mL of mixture G to 90 mL of mixture F, and mix for 30 min under magnetic stirring at a speed of 150 rpm to obtain mixture H;

[0131] Step nine: Add 2 mL of mixture D into 98 mL of mixture H at a rate of 2 mL / min, and mix for 30 min under magnetic stirring at a rotation speed of 150 rpm to obtain an insecticide and mildew-proof tobacco alcoholization composition.

[0132] Example 1-8: An insecticidal and mildew-proof tobacco alcoholization composition and its preparation method

[0133] This embodiment provides an insecticidal and mildew-proof tobacco leaf alcoholization composition, the ingredients of which include glucose oxidase, catalase, glucose, mildew inhibitor (sodium diacetate), chitosan, glycerol and acetic acid.

[0134] The preparation method of the insecticide and mildew-proof tobacco alcoholization composition comprises the following steps:

[0135] Step 1: Add 0.2 g of glucose oxidase and 0.2 g of catalase into 100 mL of distilled water, mix for 2 min under magnetic stirring at a speed of 150 rpm, and sterile filter using a filter membrane with a pore size of 0.22 μm to obtain a mixture A;

[0136] Step 2: Add 10 g of glucose into 90 mL of distilled water, mix for 2 min under magnetic stirring at a speed of 150 rpm, and sterile filter using a 0.22 μm pore size filter membrane to obtain a mixture B;

[0137] Step 3: Add 1 mL of acetic acid into 99 mL of distilled water, and mix for 2 min under magnetic stirring at a speed of 150 rpm to obtain a mixture C;

[0138] Step 4: Add 6 mL of glycerol into 94 mL of distilled water and mix for 2 min under magnetic stirring at a speed of 150 rpm to obtain a mixture D;

[0139] Step 5: Add 0.2 g of sodium diacetate into 100 mL of distilled water, and mix for 2 min under magnetic stirring at a speed of 150 rpm to obtain a mixture E;

[0140] Step 6: Add 1 g of chitosan to 99 mL of mixture C, and mix for 30 min under magnetic stirring at a speed of 150 rpm to obtain mixture F;

[0141] Step 7: Mixture A, mixture B and mixture E in a volume ratio of 1:1:1, and mix under magnetic stirring at a rotation speed of 150 rpm for 5 minutes to obtain mixture G;

[0142] Step 8: Add 10 mL of mixture G to 90 mL of mixture F, and mix for 30 min under magnetic stirring at a speed of 150 rpm to obtain mixture H;

[0143] Step nine: Add 2 mL of mixture D into 98 mL of mixture H at a rate of 2 mL / min, and mix for 30 min under magnetic stirring at a rotation speed of 150 rpm to obtain an insecticide and mildew-proof tobacco alcoholization composition.

[0144] Comparative Example 1-1: A tobacco alcoholization composition and a preparation method thereof

[0145] This comparative example provides a tobacco leaf alcoholization composition, the ingredients of which include mildew inhibitors (dimethyl fumarate, sodium diacetate and calcium propionate), chitosan, glycerol and acetic acid.

[0146] The preparation method of the tobacco alcoholization composition comprises the following steps:

[0147] Step 1: Add 1 mL of acetic acid into 99 mL of distilled water, and mix for 2 min under magnetic stirring at a speed of 150 rpm to obtain a mixture C;

[0148] Step 2: Add 6 mL of glycerol into 94 mL of distilled water and mix for 2 min under magnetic stirring at a speed of 150 rpm to obtain a mixture D;

[0149] Step 3: Add 0.05 g of dimethyl fumarate, 0.05 g of sodium diacetate and 0.05 g of calcium propionate into 100 mL of distilled water, and mix for 2 min under magnetic stirring at a speed of 150 rpm to obtain a mixture E;

[0150] Step 4: Add 1 g of chitosan to 99 mL of mixture C, and mix under magnetic stirring at a speed of 150 rpm for 30 min to obtain mixture F;

[0151] Step 5: Add 7 mL of mixture E to 93 mL of mixture F, and mix under magnetic stirring at a speed of 150 rpm for 30 min to obtain mixture H;

[0152] Step 6: Add 2 mL of mixture D into 98 mL of mixture H at a rate of 2 mL / min, and mix for 30 min under magnetic stirring at a rotation speed of 150 rpm to obtain a tobacco alcoholization composition.

[0153] Comparative Example 1-2: A tobacco alcoholization composition and a preparation method thereof

[0154] This comparative example provides a tobacco leaf alcoholization composition, the ingredients of which include glucose oxidase, catalase, glucose, chitosan, glycerol and acetic acid.

[0155] The preparation method of the tobacco alcoholization composition comprises the following steps:

[0156] Step 1: Add 0.2 g of glucose oxidase and 0.2 g of catalase into 100 mL of distilled water, mix for 2 min under magnetic stirring at a speed of 150 rpm, and sterile filter using a filter membrane with a pore size of 0.22 μm to obtain a mixture A;

[0157] Step 2: Add 10 g of glucose into 90 mL of distilled water, mix for 2 min under magnetic stirring at a speed of 150 rpm, and sterile filter using a 0.22 μm pore size filter membrane to obtain a mixture B;

[0158] Step 3: Add 1 mL of acetic acid into 99 mL of distilled water, and mix for 2 min under magnetic stirring at a speed of 150 rpm to obtain a mixture C;

[0159] Step 4: Add 6 mL of glycerol into 94 mL of distilled water and mix for 2 min under magnetic stirring at a speed of 150 rpm to obtain a mixture D;

[0160] Step 5: Add 1 g of chitosan to 99 mL of mixture C, and mix for 30 min under magnetic stirring at a speed of 150 rpm to obtain mixture F;

[0161] Step 6: Mixture A and mixture B at a volume ratio of 1:1, and mix under magnetic stirring at a rotation speed of 150 rpm for 2 minutes to obtain mixture G;

[0162] Step 7: Add 8 mL of mixture G to 92 mL of mixture F, and mix for 30 min under magnetic stirring at a speed of 150 rpm to obtain mixture H;

[0163] Step eight: Add 2 mL of mixture D into 98 mL of mixture H at a rate of 2 mL / min, and mix for 30 min under magnetic stirring at a rotation speed of 150 rpm to obtain a tobacco alcoholization composition.

[0164] Experimental Example 1: Experiment on the insecticidal and mildew-proof properties of tobacco alcoholization composition

[0165] This experimental example provides an experiment on the insecticidal and mildew-proof performance of the tobacco alcoholization composition. The experimental process is as follows:

[0166] Take 5 kg of tobacco leaves infested with insects, and spray the tobacco alcoholization compositions of Examples 1-1 to 1-8 and Comparative Examples 1-1 to 1-2 uniformly on the tobacco leaves at a ratio of 2% of the weight of the tobacco leaves, respectively. Seal them with plastic films and place them in a storage room with a temperature of 27±2°C and a humidity of 60±6%. After 60 days, observe with the naked eye whether the pests are active and whether the insect bodies are rigid, and the insect mortality rate is the percentage of the number of inactive pests or rigid insect bodies to the total number of pests. The mildew-proof effect of the tobacco alcoholization composition is detected by observing with the naked eye whether the tobacco leaves are mildewed, and the mildew rate is the percentage of the weight of the whole mildewed tobacco leaves to the total weight of the tobacco leaves. The experimental results are shown in Table 1.

[0167] As can be seen from Table 1, when the tobacco leaves were aged using the tobacco leaf ageing compositions of Examples 1-1 to 1-8, the mortality rate of pests in the tobacco leaves reached 100%, and no mildew occurred; when the tobacco leaves were aged using the tobacco leaf ageing composition of Comparative Example 1-1, since the composition did not contain glucose oxidase and catalase, the oxygen concentration could not be kept at a low level during the natural ageing of the tobacco leaves, and the pests could not be effectively killed; when the tobacco leaves were aged using the tobacco leaf ageing composition of Comparative Example 1-2, since the composition did not contain a mildew inhibitor, 10% of the tobacco leaves became mildewed during the natural ageing of the tobacco leaves.

[0168] Table 1 Insecticidal and mildew-proof performance results of tobacco alcoholization composition

[0169] Pest mortality / % Mildew rate / % Example 1-1 100 none Example 1-2 100 none Examples 1-3 100 none Examples 1-4 100 none Examples 1-5 100 none Examples 1-6 100 none Examples 1-7 100 none Examples 1-8 100 none Comparative Example 1-1 42 none Comparative Example 1-2 90 10

[0170] Obviously, the above embodiments are merely examples for the purpose of clear explanation, and are not intended to limit the implementation methods. For those skilled in the art, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to list all the implementation methods here. The obvious changes or modifications derived therefrom are still within the scope of protection of the invention.

Claims

1. An insecticidal and mildew-proof tobacco alcoholization composition, characterized in that: The tobacco alcoholization composition comprises glucose oxidase, catalase, glucose, mildew inhibitor, chitosan, glycerol, acetic acid and water; and the preparation method of the tobacco alcoholization composition comprises the following steps: Step 1: adding 0.05-0.2 g of glucose oxidase and 0.05-0.2 g of catalase into 100 mL of water, and mixing to obtain a mixture A; Step 2: Add 1-10 g of glucose into 90-99 mL of water and mix to obtain a mixture B; Step 3: Add 1 mL of acetic acid into 99 mL of water and mix to obtain a mixture C; Step 4: Add 6 mL of glycerol into 94 mL of water and mix to obtain mixture D; Step 5: Add 0.05-0.2 g of the mildew inhibitor into 100 mL of water and mix to obtain a mixture E; Step 6: Add 1 g of chitosan to 99 mL of mixture C, and mix to obtain mixture F; Step 7: Mixing mixture A, mixture B and mixture E in a volume ratio of 1:1:1 to obtain mixture G; Step 8: Add 10 mL of mixture G to 90 mL of mixture F, and mix to obtain mixture H; Step nine: adding 2 mL of the mixture D to 98 mL of the mixture H at a rate of 1 to 2 mL / min, and mixing to obtain an insecticide and mildew-proof tobacco alcoholization composition; The mildew preventer comprises one or a combination of dimethyl fumarate, sodium diacetate and calcium propionate.

2. The tobacco alcoholization composition according to claim 1, characterized in that: The rate in step nine is 2 mL / min.

3. The tobacco alcoholization composition according to claim 1, characterized in that: The glucose oxidase is food grade glucose oxidase, and the enzyme activity is ≥10000U / g.

4. The tobacco alcoholization composition according to claim 1 or 2, characterized in that: The catalase is food grade catalase, and the enzyme activity is ≥100000 U / g.

5. Use of the insecticidal and mildew-proof tobacco alcoholization composition according to any one of claims 1 to 4 in tobacco alcoholization.