A composite additive for improving lamb growth performance and its preparation method and application

Abstract

The present application relates to a composite additive for improving the growth performance of lambs, and its preparation method and application. It includes the following components: Bacillus licheniformis, Bacillus subtilis, Enterococcus faecium, rattan tea extract, and manno-oligosaccharide. The composite additive provided by the present application selects Bacillus licheniformis with antibacterial activity and cellulose degradation function, and is combined with Bacillus subtilis and Enterococcus faecium to prepare a composite probiotic, which can regulate the intestinal flora structure of lambs and promote the healthy development of their digestive tract, and further compound rattan tea extract with antibacterial, anti-inflammatory, antioxidant and immunity-enhancing effects, and prebiotic manno-oligosaccharide that increases the relative abundance of intestinal probiotics, thereby improving the immune function of lambs, promoting intestinal health, and further improving their growth performance.

Description

Technical Field

[0001] The present application relates to the technical field of lamb feed, and in particular to a composite additive for improving lamb growth performance and a preparation method and application thereof. Background Art

[0002] Mutton has the characteristics of high protein content and low fat content. It is an indispensable nourishing food in the daily diet of Chinese residents and has become an important choice for people to enrich their meat consumption and improve their dietary structure. In recent years, with the improvement of Chinese residents' income level and the upgrading of consumption structure, the demand for mutton consumption has continued to grow. The huge consumer market has provided a good opportunity for the development of the mutton breeding industry.

[0003] In production, lamb rearing is a crucial production stage in mutton sheep farming. Due to the incomplete growth and development of lamb organs, the tolerance to environmental changes and resistance to diseases are relatively poor, which can easily lead to the occurrence of diseases during breeding, thus significantly reducing their production performance and causing significant economic losses.

[0004] Therefore, breeding high-quality lambs is a prerequisite for achieving high-yield and high-efficiency mutton sheep farming. In the past, the use of feed antibiotics could effectively alleviate the negative effects of lamb weaning stress, diarrhea, etc., but with the implementation of my country's "antibiotic ban" policy for feed, the development of efficient antibiotic substitutes has become a hot topic in the breeding industry.

[0005] Therefore, in order to achieve efficient production of mutton sheep farming, it is of great significance to develop a feed additive for lambs to improve their growth performance.

[0006] Currently, there are related technologies that rely solely on various probiotics to regulate the intestinal environment of lambs. However, relying solely on probiotics has the problem of poor effect. In addition, the combined use of probiotics from different genera has potential synergistic effects. Therefore, how to choose appropriate probiotics for compound use plays a vital role in improving the growth performance of lambs. Summary of the invention

[0007] The present application provides a composite additive for improving lamb growth performance and a preparation method and application thereof, so as to solve the problem in the related art that lamb feed additives have poor effect on improving lamb growth performance.

[0008] The technical solutions provided by this application are as follows:

[0009] In a first aspect, the present application provides a composite additive for improving the growth performance of lambs, comprising the following components:

[0010] Bacillus licheniformis, Bacillus subtilis, Enterococcus faecium, rattan tea extract, manno-oligosaccharide.

[0011] In some embodiments, the composition is as follows, calculated by mass percentage:

[0012] Bacillus licheniformis 7%-10%, Bacillus subtilis 60%-65%, Enterococcus faecium 7%-10%, rattan tea extract 12%-15%, manno-oligosaccharide 5%-6%.

[0013] In some embodiments, the composition is as follows, calculated by mass percentage:

[0014] Bacillus licheniformis 8.07%, Bacillus subtilis 64.51%, Enterococcus faecium 8.07%, rattan tea extract 13.97%, manno-oligosaccharide 5.38%.

[0015] In some embodiments, the viable count of the Bacillus licheniformis is ≥ 4.0×10 10 CFU / g;

[0016] And / or, the viable count of the Bacillus subtilis is ≥ 2.0×10 10 CFU / g;

[0017] And / or, the viable count of Enterococcus faecium is ≥ 2.0×10 10 CFU / g.

[0018] In some embodiments, the total flavonoids content of the Ampelopsis ulmoides extract is greater than 40%.

[0019] In a second aspect, the present application also provides a method for preparing the composite additive for improving lamb growth performance as described above, comprising the following steps:

[0020] The composite probiotics are obtained by mixing Bacillus licheniformis, Bacillus subtilis and Enterococcus faecium;

[0021] The compound probiotics are mixed with rattan tea extract and manno-oligosaccharide to obtain a compound additive for improving the growth performance of lambs.

[0022] In a third aspect, the present application also provides the use of the composite additive for improving lamb growth performance as described above in the preparation of lamb feed.

[0023] In a fourth aspect, the present application also provides a method for improving lamb growth performance, the method comprising the following steps:

[0024] Before weaning, lambs are directly fed with the compound additives for improving lamb growth performance as described above;

[0025] After weaning, the lambs are fed with a mixed feed, which includes a basic feed and the compound additive for improving the growth performance of the lambs as described above.

[0026] In some embodiments, the mass ratio of the composite additive to the basic feed is 0.15%-0.25%.

[0027] In some embodiments, before the lamb is weaned, the feeding amount of the composite additive for improving the growth performance of the lamb is 0.5-0.8 g / lamb.

[0028] The beneficial effects of the technical solution provided by this application include:

[0029] The composite additive provided in the present application selects Bacillus licheniformis with antibacterial activity and cellulose degradation function, and is combined with Bacillus subtilis and Enterococcus faecium to prepare a composite probiotic, which can regulate the intestinal flora structure of lambs and promote the healthy development of their digestive tract. It is further compounded with rattan tea extract with antibacterial, anti-inflammatory, antioxidant and immunity-enhancing effects, and prebiotic mannan oligosaccharides that increase the relative abundance of intestinal probiotics, thereby improving the immune function of lambs, promoting intestinal health, and further improving their growth performance. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings required for use in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0031] Figure 1 This is a heat map of the intestinal microbial species composition of lambs in group A and the blank group of this application. DETAILED DESCRIPTION

[0032] In order to make the purpose, technical scheme and advantages of the embodiments of the present application clearer, the technical scheme in the embodiments of the present application will be clearly and completely described below in combination with the embodiments of the present application. Obviously, the described embodiments are part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of this application.

[0033] In a first aspect, the present application provides a composite additive for improving the growth performance of lambs, comprising the following components:

[0034] Bacillus licheniformis, Bacillus subtilis, Enterococcus faecium, rattan tea extract, manno-oligosaccharide.

[0035] The composite additive provided by the present application can increase the relative abundance of butyric acid-producing bacteria (Butyricicoccus_pullicaecorum, Butyrivibrio_crossotus) in the intestinal tract of lambs. The metabolite butyric acid produced by the fermentation of carbohydrates by butyric acid bacteria is beneficial to improving intestinal immune function, inhibiting intestinal inflammatory response, maintaining normal physiological function of the intestinal barrier, promoting the growth of beneficial intestinal flora, improving digestion and absorption of nutrients, thereby increasing the average daily weight gain of lambs, reducing feed-to-weight ratio, and increasing the economic benefits of breeding;

[0036] In addition, it can significantly reduce the activity of alanine aminotransferase in lamb serum, improve liver function, and is beneficial to the healthy development of the liver, the most important metabolic organ in the body. It can also significantly reduce the content of C-reactive protein in lamb serum, improve lamb immune function, and improve growth performance.

[0037] In some embodiments, the composition is as follows, by weight percentage:

[0038] Bacillus licheniformis 7%-10%, Bacillus subtilis 60%-65%, Enterococcus faecium 7%-10%, rattan tea extract 12%-15%, manno-oligosaccharide 5%-6%.

[0039] The composite additive provided in the present application can effectively improve the intestinal flora structure of lambs and enhance the body's immune performance through the design of the ratio of various probiotics, rattan tea extracts and manno-oligosaccharides, so that the lambs have good growth performance.

[0040] In some embodiments, the composition is as follows, by weight percentage:

[0041] Bacillus licheniformis 8.07%, Bacillus subtilis 64.51%, Enterococcus faecium 8.07%, rattan tea extract 13.97%, manno-oligosaccharide 5.38%.

[0042] In some embodiments, the viable count of Bacillus licheniformis is ≥ 4.0×10 10 CFU / g;

[0043] And / or, the viable count of the Bacillus subtilis is ≥ 2.0×10 10 CFU / g;

[0044] And / or, the viable count of Enterococcus faecium is ≥ 2.0×10 10 CFU / g.

[0045] Preferably, the Bacillus licheniformis is Bacillus licheniformis JH41C, with a deposit number of CCTCC NO: M2020646.

[0046] Preferably, the effective substance content of the mannooligosaccharide is greater than 90%.

[0047] In some embodiments, the total flavonoids content of the Ampelopsis pilosula extract is >40%.

[0048] Bacillus licheniformis and Bacillus subtilis have strong resistance to stress, high temperature and high pressure, can exist in the form of spores under adverse environmental conditions, and maintain high activity. They have good effects on regulating the balance of intestinal flora, promoting intestinal health, and improving digestion ability. Enterococcus faecium can regulate the intestinal microecological environment, reduce the diarrhea rate, and improve the utilization rate of nutrients, thereby increasing the daily weight gain of lambs and improving their growth performance. The composite additive contains Bacillus licheniformis, Bacillus subtilis and Enterococcus faecium at the same time, and their effects can promote each other to achieve synergistic synergy, promote growth and development, and increase daily weight gain.

[0049] Tengcha extract has antibacterial, anti-inflammatory, antioxidant and immunity-enhancing effects. The prebiotic mannan oligosaccharide can increase the relative abundance of intestinal probiotics, thereby improving the immune function of lambs, promoting intestinal health, and further improving their growth performance.

[0050] In a second aspect, the present application also provides a method for preparing the composite additive for improving lamb growth performance as described above, comprising the following steps:

[0051] The composite probiotics are obtained by mixing Bacillus licheniformis, Bacillus subtilis and Enterococcus faecium;

[0052] The compound probiotics are mixed with rattan tea extract and manno-oligosaccharide to obtain a compound additive for improving the growth performance of lambs.

[0053] In a third aspect, the embodiments of the present application also provide the use of the composite additive for improving lamb growth performance as described above in the preparation of lamb feed.

[0054] In a fourth aspect, the present application also provides a method for improving lamb growth performance, the method comprising the following steps:

[0055] Before weaning, lambs are directly fed with the compound additives for improving lamb growth performance as described above;

[0056] After weaning, the lambs are fed with a mixed feed, which includes a basic feed and the compound additive for improving the growth performance of the lambs as described above.

[0057] In some embodiments, the mass ratio of the composite additive to the basic feed is 0.15%-0.25%.

[0058] In some embodiments, before the lamb is weaned, the feeding amount of the composite additive for improving the growth performance of the lamb is 0.5-0.8 g / lamb.

[0059] The present application is further described below through specific examples.

[0060] Example 1

[0061] This embodiment provides a composite additive for improving the growth performance of lambs, which comprises the following components by mass percentage:

[0062] Bacillus licheniformis 8.07%, Bacillus subtilis 64.51%, Enterococcus faecium 8.07%, rattan tea extract 13.97%, manno-oligosaccharide 5.38%.

[0063] Comparative Example 1

[0064] Same as Example 1, except that Bacillus licheniformis is not included.

[0065] Comparative Example 2

[0066] Same as Example 1, except that Bacillus subtilis is not included.

[0067] Comparative Example 3

[0068] Same as Example 1, except that Enterococcus faecium is not included.

[0069] Comparative Example 4

[0070] Same as Example 1, except that the rattan tea extract is not included.

[0071] Comparative Example 5

[0072] Same as Example 1, except that manno-oligosaccharide is not included.

[0073] Effect verification

[0074] 1. Experimental animals and groups

[0075] A total of 126 naturally delivered, 7-day-old Matou goat lambs with similar body weight (4.42±0.22kg) were selected from a breeding farm and randomly divided into 7 groups, including 1 blank group and 6 experimental groups. The experimental groups were labeled AF groups, and each group was set up with 3 parallel replicates, with 6 lambs in each replicate.

[0076] 2. Feeding and management

[0077] All lambs were nursed in the same pen with their ewes, and were fed with starter feed in the supplementary feeding pen from the age of 7 days. The starter feed consisted of 50% corn, 22% soybean meal, 19% alfalfa, 5% bran, 2% stone powder, 0.5% calcium hydrogen phosphate, 0.5% salt, and 1% premix by mass percentage. During the experiment, lambs were free to eat and drink clean water, and no antibiotics were used throughout the experiment. The experiment lasted for 60 days.

[0078] Before weaning, the compound additive was dissolved in warm water at 0.7 g per lamb at 8:00 a.m. every day and used to feed the lambs in the experimental group, while the lambs in the control group were only fed with warm water without the compound additive;

[0079] Weaning was carried out at 49 days, and the ewes and lambs were separated. The lambs were kept in the original pens. The lambs in the experimental group were fed a basal diet containing a compound additive (added to the basal diet at 0.2% of the dry matter intake), while the lambs in the control group were only fed a basal diet.

[0080] The differences among the experimental groups are as follows:

[0081] Group A, fed with the composite additive provided in Example 1;

[0082] Group B was fed with the compound additive provided in Comparative Example 1;

[0083] Group C was fed with the compound additive provided in Comparative Example 2;

[0084] Group D was fed with the compound additive provided in Comparative Example 3;

[0085] Group E was fed with the compound additive provided in Comparative Example 4;

[0086] Group F was fed with the compound additive provided in Comparative Example 5.

[0087] 3. Test results

[0088] The experimental data of the following experiments were statistically analyzed using SPSS16.0 software, and the results were expressed as mean±standard deviation. The two groups of data were compared using t-test, with P<0.05 indicating a significant difference, and P<0.01 indicating an extremely significant difference.

[0089] (1) Growth performance

[0090] During the experiment, the daily feed intake of the lambs was recorded, and all the experimental lambs were weighed before and after the experiment to determine the average daily gain (ADG), average daily feed intake (ADFI), and feed-to-weight ratio (F / G). The results are shown in Table 1.

[0091] Table 1: Growth performance test results

[0092]

[0093] Note: In the same row, when comparing the blank group with the experimental group, different lowercase letters in the shoulder indicate significant difference (P<0.05), while no shoulder indicates no significant difference (P>0.05), the same below.

[0094] According to the test results in Table 1, it can be seen that the lambs in Group A were fed the composite additive provided in Example 1 of the present application. Compared with the blank group, their final body weight increased by 16.7% (P<0.05), the average daily weight gain increased by 26.23% (P<0.05), the average daily feed intake increased by 13.55% (P>0.05), and the feed-to-weight ratio decreased by 9.51% (P>0.05), indicating that the composite additive of the present application can improve the growth performance of lambs.

[0095] (2) Blood indicators

[0096] At the end of the experiment, 10 lambs were randomly selected from the blank group and group A for jugular vein blood collection, serum separation, and routine biochemical indices. Total protein (TP), albumin (ALB), globulin (GLOB), alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), urea nitrogen (UREA), triglyceride (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDLC), low-density lipoprotein cholesterol (LDLC), glucose (GLU), and C-reactive protein (CRP) in the test sheep serum were measured using a fully automatic biochemical analyzer (Canon TBA120FR, Japan). The results are shown in Table 2.

[0097] Table 2: Blood index test results

[0098] project blank A group TP(g / L) 64.53±3.46 63.25±4.98 ALB(g / L) 30.74±3.04 29.17±3.17 GLOBAL (g / L) 33.79±2.61 34.08±5.96 ALT(U / L) <![CDATA[20.70 a ±3.70]]> <![CDATA[15.79 b ±3.66]]> AST(U / L) 114.71±8.45 101.13±11.88 ALP(U / L) 338.33±102.61 320.18±117.47 UREA (mmol / L) 3.28±0.53 3.59±0.33 TG(mmol / L) 0.39±0.04 0.37±0.05 TC(mmol / L) 1.98±0.18 1.85±0.19 HDLC(mmol / L) 1.03±0.17 0.94±0.13 LDLC(mmol / L) 0.71±0.13 0.77±0.24 CRP(mmol / L) <![CDATA[0.51 a ±0.13]]> <![CDATA[0.33 b ±0.16]]>

[0099] The determination of serum biochemical indices can reflect the health status of the body to a certain extent. ALT and AST are mainly present in liver tissue cells and are sensitive indicators of liver cell damage. As shown in Table 2, the ALT activity in the serum of lambs in group A was significantly lower than that in the blank group (P<0.05), indicating that the composite additive of the present application has a certain effect of improving liver function and is beneficial to the healthy development of lamb liver; at the same time, the serum CRP content of lambs in group A was significantly lower than that in the blank group (P<0.05). CRP is an acute phase protein synthesized by the liver and is a sensitive indicator of the body's inflammatory response. The serum CRP content is usually used as an effective indicator for evaluating the activity and severity of inflammation, thus indicating that the addition of the composite additive of the present application can improve the immune function of lambs.

[0100] (3) Determination of intestinal microbial flora

[0101] At the end of the experiment, two lambs in each group of the blank group and group A were randomly selected to collect fresh feces samples, which were placed in cryopreservation tubes and stored in a -80°C refrigerator for DNA extraction. PCR amplification was performed on the bacterial 16S rDNA V3-V4 region. After the amplified products were tested and qualified, a library was constructed and sequenced using Illumina Novaseq PE250. After the data were downloaded, bioinformatics methods were used to analyze the composition and structure of the flora. The results are shown in Figure 1 and Table 3.

[0102] Table 3: Relative abundance of intestinal microorganisms in lambs (%)

[0103] ID blank A group Campylobacter_fetus 2.0971 1.6036 Streptococcus_luteciae 0.0153 1.8964 Clostridium_celatum 0.1955 0.395 Ruminococcus_flavefaciens 0.2015 0.2702 Oscillospira_guilliermondii 0.0889 0.0534 Defluviitalea_saccharophila 0.0688 0.0643 Clostridium_neonatale 0.0518 0.0616 Methylobacterium_komagatae 0.0633 0.0356 Butyricicoccus_pullicaecorum 0.0259 0.0563 Roseburia_faeces 0.0553 0.0142 Sphingomonas_echinoides 0.0433 0.0219 Clostridium_sordellii 0.0156 0.0446 Akkermansia_muciniphila 0.0533 0 Bacillus_muralis 0.0352 0.016 Ruminococcus_albus 0.0192 0.0274 Butyrivibrio_crossotus 0.00818 0.037 Faecalibacterium_prausnitzii 0.0332 0.00848 Alistipes_finegoldii 0.00104 0.0397 Haemophilus_parainfluenzae 0.019 0.0205 Coprococcus_catus 0.0348 0 Others 96.8736 95.3337

[0104] According to Table 3 and Figure 1 , the relative abundance of butyrate-producing bacteria (such as Butyricicoccus_pullicaecorum, Butyrivibrio_crossotus) in the intestine of lambs in group A is higher than that in the blank group. Butyrate, the metabolite produced by fermenting carbohydrates in the intestine, can promote the regeneration and repair of intestinal epithelial cells, maintain the normal physiological function of the intestinal barrier, and has the effect of inhibiting intestinal inflammation, thereby improving intestinal immune function. In addition, it can promote the growth of beneficial flora (such as bifidobacteria and lactobacilli) in the animal intestine, inhibit the reproduction of intestinal pathogens, and thus regulate the structure of intestinal flora. Therefore, it is shown that the composite additive of this application can regulate the development of probiotic flora in the intestinal tract of lambs and promote their intestinal health.

[0105] 4. Conclusion

[0106] The composite additive provided by the present application can significantly increase the average daily weight gain of lambs by 26.23% (P<0.05), reduce the feed-to-weight ratio by 9.51% (P>0.05), and significantly reduce the activity of serum alanine aminotransferase and the content of C-reactive protein (P<0.05), and increase the relative abundance of probiotics such as intestinal butyrate-producing bacteria. Therefore, it is shown that the composite additive of the present application can improve the immune function of lambs, regulate the structure of intestinal flora, and promote intestinal health, thereby improving their growth performance and improving the economic benefits of mutton sheep farming.

[0107] 5. Comparison of experimental groups

[0108] (1) Group A and Group B were tested according to the method of “Growth performance” under “3. Test results”. The results are shown in Table 4.

[0109] Table 4 Effects on growth performance

[0110]

[0111] According to the test results in Table 4, it can be seen that the lambs in Group B were fed with the composite additive provided in Comparative Example 1 of the present application. Compared with Group A, their final body weight decreased by 6.45% (P<0.05), the average daily weight gain during the whole period decreased by 7.78% (P<0.05), the average daily feed intake did not change significantly (P>0.05), and the feed-to-weight ratio increased by 8.72% (P<0.05), indicating that the effect of feeding the composite additive in Example 1 of the present application is better than that in Comparative Example 1.

[0112] (2) Group A and Group C were tested according to the method of “Growth performance” under “3. Test results”. The results are shown in Table 5.

[0113] Table 5 Effects on growth performance

[0114]

[0115] According to the test results in Table 5, it can be seen that the lambs in Group C were fed with the composite additive provided in Comparative Example 2 of the present application. Compared with Group A, their final body weight decreased by 9.36% (P<0.05), the average daily weight gain during the whole period decreased by 13.92% (P<0.05), the average daily feed intake decreased by 3.30% (P>0.05), and the feed-to-weight ratio increased by 12.31% (P<0.05), indicating that the effect of feeding the composite additive in Example 1 of the present application is better than that in Comparative Example 2.

[0116] (3) Group A and Group D were tested according to the method of “Growth Performance” under “3. Test Results”. The results are shown in Table 6.

[0117] Table 6 Effects on growth performance

[0118]

[0119]

[0120] According to the test results in Table 6, it can be seen that the lambs in Group D were fed with the composite additive provided in Comparative Example 3 of the present application. Compared with Group A, their final body weight decreased by 3.09% (P>0.05), the average daily weight gain during the whole period decreased by 3.66% (P>0.05), the average daily feed intake decreased by 1.61% (P>0.05), and the feed-to-weight ratio increased by 2.05% (P>0.05), indicating that the effect of feeding the composite additive in Example 1 of the present application is better than that in Comparative Example 3.

[0121] (4) Group A and Group E were tested according to the method of “Growth performance” under “3. Test results”. The results are shown in Table 7.

[0122] Table 7 Effects on growth performance

[0123]

[0124] According to the test results in Table 7, it can be seen that the lambs in Group E were fed with the composite additive provided in Comparative Example 4 of the present application. Compared with Group A, their final body weight decreased by 4.86% (P<0.05), and their average daily weight gain during the whole period decreased by 4.35% (P>0.05), indicating that the effect of feeding the composite additive in Example 1 of the present application is better than that in Comparative Example 4.

[0125] (5) Group A and Group F were tested according to the method of “Growth performance” under “3. Test results”. The results are shown in Table 8.

[0126] Table 8 Effects on growth performance

[0127]

[0128] According to the test results in Table 8, it can be seen that the lambs in Group F were fed with the composite additive provided in Comparative Example 5 of the present application. Compared with Group A, their final body weight decreased by 4.15% (P>0.05), the average daily weight gain during the whole period decreased by 6.47% (P<0.05), the average daily feed intake decreased by 2.60% (P>0.05), and the feed-to-weight ratio increased by 4.10% (P>0.05), indicating that the effect of feeding the composite additive in Example 1 of the present application is better than that in Comparative Example 5.

[0129] It should be noted that, in this application, the terms "include", "comprises" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device. In the absence of further restrictions, an element defined by the sentence "includes a ..." does not exclude the existence of other identical elements in the process, method, article or device including the element.

[0130] The above description is only a specific implementation of the present application, so that those skilled in the art can understand or implement the present application. Various modifications to these embodiments will be apparent to those skilled in the art, and the general principles defined herein can be implemented in other embodiments without departing from the spirit or scope of the present application. Therefore, the present application will not be limited to the embodiments shown herein, but will conform to the widest range consistent with the principles and novel features applied for herein.

Claims

1. A method for preparing a composite additive for improving lamb growth performance, characterized in that: The composite additive for improving the growth performance of lambs is composed of 8.07% of Bacillus licheniformis, 64.51% of Bacillus subtilis, 8.07% of Enterococcus faecium, 13.97% of rattan tea extract, and 5.38% of manno-oligosaccharide, by mass percentage; The preparation method comprises the following steps: The composite probiotics are obtained by mixing Bacillus licheniformis, Bacillus subtilis and Enterococcus faecium; The compound probiotics are mixed with rattan tea extract and manno-oligosaccharide to obtain a compound additive for improving the growth performance of lambs.

2. The method for preparing the composite additive for improving lamb growth performance according to claim 1, characterized in that: The viable count of the Bacillus licheniformis is ≥ 4.0×10 10 CFU / g; And / or, the viable count of the Bacillus subtilis is ≥ 2.0×10 10 CFU / g; And / or, the viable count of Enterococcus faecium is ≥ 2.0×10 10 CFU / g.

3. The method for preparing the composite additive for improving lamb growth performance according to claim 1, characterized in that: The total flavonoids content of the rattan tea extract is greater than 40%.

4. Use of a composite additive for improving lamb growth performance in preparing lamb feed, wherein the composite additive for improving lamb growth performance is prepared by the preparation method of the composite additive for improving lamb growth performance as claimed in claim 1.

5. A method for improving lamb growth performance, characterized in that: The method comprises the following steps: Before weaning, lambs are directly fed with compound additives that improve lamb growth performance; After weaning, the lambs are fed with mixed feed, which includes basic feed and compound additives for improving the growth performance of the lambs; Wherein, the composite additive for improving the growth performance of lambs is prepared by the preparation method of the composite additive for improving the growth performance of lambs as claimed in claim 1.

6. The method for improving lamb growth performance according to claim 5, characterized in that: The mass ratio of the composite additive to the basic feed is 0.15%-0.25%.

7. The method for improving lamb growth performance according to claim 5, characterized in that: Before the lamb is weaned, the feeding amount of the composite additive for improving the growth performance of the lamb is 0.5-0.8 g / lamb.

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