Infant formula milk powder and preparation method thereof

Abstract

The present invention provides an infant formula milk powder and a preparation method thereof. The infant formula milk powder contains a protein composition capable of improving the digestibility of protein in the infant formula milk powder; wherein, based on the total protein content of the infant formula milk powder as 100%, the protein composition contains 13.0%-50.5% of α-lactalbumin and 15.0%-35.0% of β-casein. The technical solution of the present invention can significantly improve the digestibility of whey protein in infant formula milk powder.

Description

Technical Field

[0001] The invention belongs to the technical field of infant formula food, and specifically relates to a formula milk powder and a preparation method thereof, in particular to a formula milk powder containing a protein composition for improving protein digestibility in food and a preparation method thereof. Background Art

[0002] Among the three major energy-supplying nutrients, protein has aroused great interest due to its important roles for mammalian cubs, such as providing energy, promoting height, inhibiting bacteria and infection, and promoting mineral absorption.

[0003] The huge difference in protein composition between cow's milk and human milk reflects the differences in physiological characteristics and growth patterns of different species, and the nutritional value they bring is also different.

[0004] Generally speaking, the protein in food can only be absorbed after being hydrolyzed into amino acids and short peptides. Since saliva does not contain enzymes that hydrolyze proteins, protein digestion starts in the stomach, but mainly occurs in the small intestine. The enzyme that digests protein in the stomach is pepsin, which breaks down protein into peptones and a small amount of polypeptides and amino acids. The most suitable pH value for pepsin to work is 1.8-3.5. Pepsin has a coagulation effect on casein, and after forming curds, it stays in the stomach for a long time, which is conducive to digestion and absorption. This is very important for infants. The digestion of protein in the small intestine is mainly completed by various proteases secreted by the pancreas (such as trypsin, etc.), which can quickly degrade the protein in the small intestine into amino acids and short peptides that can be absorbed by the small intestinal epithelial cells.

[0005] It is generally believed that casein will clump in the stomach, resulting in a long stay time in the stomach, which prolongs the emptying time of the stomach and the digestion and absorption time of the protein. Under the combined action of the strong acidic environment and digestive enzymes in the stomach, the casein micelles are destroyed and re-coact with acid and pepsin to form clots. The size of the clots can be reduced by increasing the proportion of β-casein. However, because the spatial structure of whey protein in the stomach is not easily destroyed, existing studies have reported that whey protein is not easily digested in the stomach. It can only be completely degraded into free amino acids after entering the small intestine and absorbed for use by the body. There is a difference in the spatial structure of α-lactalbumin and β-lactoglobulin. It is easier to form a sparse structure in the presence of gastric acid. Therefore, whether the digestibility can be improved by increasing the proportion of α-lactalbumin is a very worthy issue to discuss.

[0006] Although protein has the above-mentioned advantages and digestion characteristics, the digestibility of protein-containing foods such as ordinary infant formula milk powder is still not as good as breast milk during the digestion process of infants and young children, which needs to be improved urgently. Summary of the invention

[0007] In order to solve the above technical problems, the purpose of the present invention is to provide an infant formula milk powder, which is a protein composition that can improve the protein digestibility. The formula milk powder can improve the protein digestibility, make the protein in the infant formula milk powder easier to digest and absorb, and be closer to the effect of breastfeeding.

[0008] To achieve the above object, the present invention provides an infant formula milk powder, which contains a protein composition capable of improving the digestibility of protein in the infant formula milk powder; wherein, based on the total protein content of the infant formula milk powder as 100%, the protein composition contains 13.0%-50.5% of α-lactalbumin and 15.0%-35.0% of β-casein. The infant formula milk powder controls the composition and content range of the protein in the infant formula milk powder, so that the protein has good digestibility, thereby promoting the digestion and absorption of milk powder.

[0009] The protein composition of the present invention, which can significantly improve the digestibility of protein (especially whey protein) in infant formula milk powder, is achieved by controlling the content of α-lactalbumin (the proportion of total protein) and the content of β-casein (the proportion of total protein) in the infant formula milk powder.

[0010] According to a specific embodiment of the present invention, improving the digestibility of protein in the present invention refers to improving the digestibility of protein from animal sources, such as protein from animal milk, especially protein from cow's milk.

[0011] According to a specific embodiment of the present invention, preferably, the improving the protein digestibility in infant formula milk powder includes at least one of the following: improving the digestibility of whey protein, increasing the types of released free amino acids, increasing the proportion of released essential amino acids EAA in the released free amino acids, increasing the production of small molecule peptides, increasing the types of characteristic peptide segments produced, in particular increasing the proportion of released essential amino acids EAA in the released free amino acids.

[0012] According to a specific embodiment of the present invention, the protein composition is a composition that can improve the protein digestibility in infant formula milk powder, and can be used to implement the method for improving the protein digestibility in infant formula milk powder provided by the present invention, as a component combined with other ingredients to obtain infant formula milk powder.

[0013] According to a specific embodiment of the present invention, preferably, based on the total protein content of the infant formula milk powder being 100%, the protein composition contains about 15.0%-50.5% of α-lactalbumin.

[0014] According to a specific embodiment of the present invention, preferably, based on the total protein content of the infant formula milk powder as 100%, the protein composition contains about 18.0%-35.0% β-casein, more preferably about 22.0%-32.0%, and further preferably about 25.0%-32.0%.

[0015] According to a specific embodiment of the present invention, preferably, based on the total protein content of the infant formula milk powder being 100%, the protein composition contains about 20.0%-35.0% of α-lactalbumin and about 26.0%-32.0% of β-casein.

[0016] According to a specific embodiment of the present invention, preferably, based on the total protein content of the infant formula milk powder as 100%, the protein composition contains about 21.0%-32.0% of alpha-lactalbumin and about 29.0%-32.0% of beta-casein. For example, based on the total protein content of the infant formula milk powder as 100%, the protein composition contains about 32.0% of alpha-lactalbumin and about 32.0% of beta-casein, or, the protein composition contains about 29.0% of alpha-lactalbumin and about 32.0% of beta-casein, or, the protein composition contains about 21.5% of alpha-lactalbumin and about 29.0% of beta-casein, or, the protein composition contains about 21.0% of alpha-lactalbumin and about 26.0% of beta-casein.

[0017] According to a specific embodiment of the present invention, preferably, based on the total protein content of the infant formula milk powder as 100%, the content of whey protein in the protein composition is about 33.0% or more, for example, 35.0%-90.0%, preferably about 40.0%-80.0%, more preferably about 50.0%-75.0%, and further preferably about 60.0%-70.0%.

[0018] According to a specific embodiment of the present invention, preferably, based on the total protein content of the infant formula milk powder as 100%, the casein content in the protein composition is controlled to be above about 15.0%, for example, 15.0%-65.0%, preferably about 28.0%-60.0%, more preferably about 32.0%-55.0%, and further preferably about 38.0%-40.0%.

[0019] The present invention can simultaneously control the content of whey protein and the content of casein in the protein composition. Preferably, the content of whey protein in the protein composition is controlled to be about 40.0%-80.0%, and the content of casein is controlled to be about 28.0%-60.0%, for example, 60.0% and 40.0%, 50.0% and 50.0%, 70.0% and 30.0%.

[0020] According to a specific embodiment of the present invention, preferably, based on 1000 parts by weight of infant formula milk powder, the added amount of the protein composition is 540-690 parts by weight.

[0021] According to a specific embodiment of the present invention, preferably, the total protein content of the infant formula milk powder is about 10.00-23.00 g / 100 g, for example, 10.00 g / 100 g, 11.00 g / 100 g, 12.00 g / 100 g, 13.00 g / 100 g, 14.00 g / 100 g, 15.00 g / 100 g, 16.00 g / 100 g, 17.00 g / 100 g, 18.00 g / 100 g, 19.00 g / 100 g, 20.00 g / 100 g, 21.00 g / 100 g, 22.00 g / 100 g, 23.00 g / 100 g, and the total protein content may also be a range consisting of the above specific contents as endpoints, for example 10.00-11.00g / 100g, 10.00-12.00g / 100g, 10.00-13.00g / 100g, 10.00-14 .00g / 100g, 10.00-15.00g / 100g, 10.00-16.00g / 100g, 10.00-17.00g / 100 g, 10.00-18.00g / 100g, 10.00-19.00g / 100g, 10.00-20.00g / 100g, 10.00- 21.00g / 100g, 10.00-22.00g / 100g, 11.00-12.00g / 100g, 11.00-13.00g / 10 0g, 11.00-14.00g / 100g, 11.00-15.00g / 100g, 11.00-16.00g / 100g, 11.00 -17.00g / 100g, 11.00-18.00g / 100g, 11.00-19.00g / 100g, 11.00-20.00g / 100g, 11.00-21.00g / 100g, 11.00-22.00g / 100g, 11.00-23.00g / 100g, 12. 00-13.00g / 100g, 12.00-14.00g / 100g, 12.00-15.00g / 100g, 12.00-16.00g / 100g, 12.00-17.00g / 100g, 12.00-18.00g / 100g, 12.00-19.00g / 100g, 12.00-20.00g / 100g, 12.00-21.00g / 100g, 12.00-22.00g / 100g, 12.00-23.0 0g / 100g, 13.00-14.00g / 100g, 13.00-15.00g / 100g, 13.00-16.00g / 100g, 13.00-17.00g / 100g, 13.00-18.00g / 100g, 13.00-19.00g / 100g, 13.00-20.00g / 100g, 13.00-21.00g / 100g, 13.00-22.00g / 100g, 13.00-23.00g / 100g, 14.00-15.00g / 100g, 14.00-16.00g / 100g, 14.00-17.0 0g / 100g, 14.00-18.00g / 100g, 14.00-19.00g / 100g, 14.00-20.00g / 100g, 14.00-21.00g / 100g, 14.00-22.00g / 100g, 14.00-23.00 g / 100g, 15.00-16.00g / 100g, 15.00-17.00g / 100g, 15.00-18.00g / 100g, 15.00-19.00g / 100g, 15.00-20.00g / 100g, 15.00-21.00g / 100g, 15.00-22.00g / 100g, 15.00-23.00g / 100g, 16.00-17.00g / 100g, 16.00-18.00g / 100g, 16.00-19.00g / 100g, 16.00-20.00g / 1 00g, 16.00-21.00g / 100g, 16.00-22.00g / 100g, 16.00-23.00g / 100g, 17.00-18.00g / 100g, 17.00-19.00g / 100g, 17.00-20.00g / 10 0g, 17.00-21.00g / 100g, 17.00-22.00g / 100g, 17.00-23.00g / 100g, 18.00-19.00g / 100g, 18.00-20.00g / 100g, 18.00-21.00g / 100 g, 18.00-22.00g / 100g, 18.00-23.00g / 100g, 19.00-20.00g / 100g, 19.00-21.00g / 100g, 19.00-22.00g / 100g, 19.00-23.00g / 100g , 20.00-21.00g / 100g, 20.00-22.00g / 100g, 21.00-23.00g / 100g, 21.00-22.00g / 100g, 21.00-23.00g / 100g, 22.00-23.00g / 100g, etc. .

[0022] According to a specific embodiment of the present invention, preferably, the content of α-lactalbumin in the infant formula milk powder is about 1.50-5.30 g / 100 g, and the content of β-casein is about 2.00-5.20 g / 100 g.

[0023] According to a specific embodiment of the present invention, the infant formula milk powder may contain different amounts of α-lactalbumin and β-casein, wherein:

[0024] The content of α-lactalbumin can be 1.50 g / 100 g, 2.00 g / 100 g, 2.20 g / 100 g, 2.40 g / 100 g, 3.00 g / 100 g, 3.30 g / 100 g, 4.00 g / 100 g, 4.60 g / 100 g, 5.00 g / 100 g, 5.30 g / 100 g; the content of α-lactalbumin can also be a range with the above specific contents as endpoint combinations, for example, 1.50-2.00 g / 100 g, 1.50-2.20 g / 100 g, 1.50-2.40 g / 100 g, 1.50-3.00g / 100g, 1.50-3.30g / 100g, 1.50-4.00g / 100g, 1.50-4.60g / 100g, 1.50-5.00g / 100g, 1.50-5.30g / 100g, 2.00- 2.20g / 100g, 2.00-2.40g / 100g, 2.00-3.00g / 100g, 2.00-3.30g / 100g, 2.00-4.00g / 100g, 2.00-4.60g / 100g, 2.00-5.00g / 100g, 2.00-5.30g / 100g, 2.20-2.40g / 100g, 2.20-3.00g / 100g, 2.20-3.30g / 100g, 2.20-4.00g / 100g, 2.20-4.60g / 100g, 2.20-5.00g / 100g, 2.20-5.30g / 100g, 2.40-3.00g / 100g, 2.40-3.30g / 100g, 2.40-4.00g / 100g, 2.40-4.60g / 100g, 2.40-5 .00g / 100g, 2.40-5.30g / 100g, 3.00-3.30g / 100g, 3.00-4.00g / 100g, 3.30-4.60g / 100g, 3.30-5.00g / 100g, 3.30-5.30g / 100g, 4.00-4.60g / 100g, 4.00-5.00g / 100g, 4.00-5.30g / 100g, 4.60-5.00g / 100g, 4.60-5.30g / 100g, 5.00-5.30g / 100g, etc.;

[0025] The content of β-casein can be 2.00 g / 100 g, 2.50 g / 100 g, 3.00 g / 100 g, 3.50 g / 100 g, 4.00 g / 100 g, 4.50 g / 100 g, 5.00 g / 100 g, 5.20 g / 100 g; the content of β-casein can also be a range with the above specific contents as endpoint combinations, for example, 2.00-2.50 g / 100 g, 2 .00-3.00g / 100g, 2.00-3.50g / 100g, 2.00-4.00g / 100g, 2.00-4.50g / 100g, 2.00-5.00 g / 100g, 2.00-5.20g / 100g, 2.50-3.00g / 100g, 2.50-3.50g / 100g, 2.50-4.00g / 100g, 2 .50-4.50g / 100g, 2.50-5.00g / 100g, 2.50-5.20g / 100g, 3.00-3.50g / 100g, 3.00-4.00 g / 100g, 3.00-4.50g / 100g, 3.00-5.00g / 100g, 3.00-5.20g / 100g, 3.50-4.00g / 100g, 3 .50-4.50g / 100g, 3.50-5.00g / 100g, 3.50-5.20g / 100g, 4.00-4.50g / 100g, 4.00-5.00g / 100g, 4.00-5.20g / 100g, 4.50-5.00g / 100g, 4.50-5.20g / 100g, 5.00-5.20g / 100g, etc.

[0026] The contents of the above-mentioned α-lactalbumin and β-casein can be combined or other specific combinations, for example: the content of α-lactalbumin is 2.38 g / 100 g, and the content of β-casein is 3.20 g / 100 g; or, the content of α-lactalbumin is 3.343 g / 100 g, and the content of β-casein is 4.13 g / 100 g; or, the content of α-lactalbumin is 3.43 g / 100 g. , the content of β-casein is 4.61g / 100g; or, the content of α-lactalbumin is 4.642g / 100g, and the content of β-casein is 5.122g / 100g; or, the content of α-lactalbumin is 5.117g / 100g, and the content of β-casein is 5.127g / 100g; or, the content of α-lactalbumin is 1.795g / 100g, and the content of β-casein is The content of α-lactalbumin is 2.386 g / 100 g; or, the content of α-lactalbumin is 2.224 g / 100 g, and the content of β-casein is 3.036 g / 100 g; or, the content of α-lactalbumin is 1.572 g / 100 g, and the content of β-casein is 2.102 g / 100 g; or, the content of α-lactalbumin is 2.208 g / 100 g, and the content of β-casein is 3.027 g / 100 g. g / 100g; or, the content of α-lactalbumin is 3.355g / 100g, and the content of β-casein is 3.363g / 100g; or, the content of α-lactalbumin is 1.585g / 100g, and the content of β-casein is 2.097g / 100g; or, the content of α-lactalbumin is 5.264g / 100g, and the content of β-casein is 2.108g / 100g, etc.

[0027] According to a specific embodiment of the present invention, preferably, the protein composition is prepared from one or more raw materials including liquid milk (such as milk), milk powder, whey protein powder, and casein powder, and the above raw materials can also be derived from whey powder, lactoferrin, hydrolyzed protein, etc.; the liquid milk includes whole milk and / or skim milk; the raw materials of the protein composition can also contain lactose. Among them, the milk powder preferably includes whole milk powder (or whole milk powder) and / or skim milk powder (or skim milk powder); the whey protein powder preferably includes concentrated whey protein powder with different protein contents and whey protein powder with different α-lactalbumin contents; the casein powder preferably includes casein powder with different β-casein contents. Among them, milk powder, whey protein powder, and casein powder are used as sources of protein, and their addition amounts are based on the requirements of the content range of whey protein and the content range of casein. Whey protein powder and casein powder are used as sources of α-lactalbumin and β-casein, respectively, and their respective addition amounts are based on the requirements of the respective contents of α-lactalbumin and β-casein.

[0028] The protein composition used in the infant formula milk powder of the present invention can be a composition prepared by pre-preparing various raw materials and then added to the infant formula milk powder as a component, or various raw materials constituting the protein composition can be added to the infant formula milk powder separately.

[0029] The protein composition used in the infant formula milk powder of the present invention can be obtained by mixing various raw materials. Specifically, the preparation method of the protein composition used in the infant formula milk powder of the present invention comprises mixing at least one of the following protein powders and / or milk powders:

[0030] (1) Whey protein powder having an α-lactalbumin content of 25% to 55% may be referred to as whey protein powder with a low α-lactalbumin content;

[0031] (2) Whey protein powder with an α-lactalbumin content of 75%-95% can be called whey protein powder with a high α-lactalbumin content;

[0032] (3) Whey protein powder with a β-casein content of 35%-70% can be called whey protein powder with low β-casein content;

[0033] (4) Whey protein powder with a β-casein content of 40%-80% can be called whey protein powder with a high β-casein content;

[0034] (5) whey protein powder having a protein content of 60% to 95%;

[0035] (6) Milk powder with a protein content of 15%-50% (preferably skimmed milk powder).

[0036] According to a specific embodiment of the present invention, preferably, the whey protein powder (1) with low α-lactalbumin content used in the preparation of the protein composition has an α-lactalbumin content of 30%-50%, more preferably 35%-45%, and further preferably 38%-42%.

[0037] According to a specific embodiment of the present invention, preferably, the α-lactalbumin content of the whey protein powder (2) with a high α-lactalbumin content used in the preparation of the protein composition is 80%-95%, more preferably 85%-95%, and further preferably 90%-95%.

[0038] According to a specific embodiment of the present invention, preferably, the β-casein content of the whey protein powder (3) with low β-casein content used in the preparation of the protein composition is 40%-65%, more preferably 45%-60%, and further preferably 50%-55%.

[0039] According to a specific embodiment of the present invention, preferably, the β-casein content of the whey protein powder (4) with a high β-casein content used in the preparation of the protein composition is 45%-80%, more preferably 50%-75%, further preferably 55%-70%, and further preferably 60%-65%.

[0040] According to a specific embodiment of the present invention, preferably, the protein content of the whey protein powder (5) used in the preparation of the protein composition is 65%-90%, more preferably 70%-85%, and further preferably 75%-80%.

[0041] According to a specific embodiment of the present invention, preferably, the protein content of the milk powder (6) used in the preparation of the protein composition is 20%-45%, more preferably 25%-40%, and further preferably 30%-35%.

[0042] The specific content ranges of the whey protein powder with low α-lactalbumin content (1), the whey protein powder with high α-lactalbumin content (2), the whey protein powder with low β-casein content (3), the whey protein powder with high β-casein content (4), the whey protein powder (5), and the milk powder (6) listed above can be used in any combination and are not limited to the combinations specifically listed above.

[0043] According to a specific embodiment of the present invention, preferably, the method for preparing the protein composition comprises mixing at least one of the following protein powders and / or milk powders:

[0044] Whey protein powder with 41% alpha-lactalbumin, such as the product from Denmark's Arla;

[0045] Whey protein powder with 92.3% α-lactalbumin, such as the product from Angup;

[0046] Whey protein powder with 53% beta-casein, such as the product from Kerry, Ireland;

[0047] Whey protein powder with a 63.2% beta-casein content, such as the product from Denmark's Arla;

[0048] Whey protein concentrate (WPC80) with 80% protein content, such as the product of Wheyco of Germany;

[0049] Skimmed milk powder with a protein content of 33.5%, such as Fonterra's product.

[0050] According to a specific embodiment of the present invention, in the above-mentioned infant formula milk powder, based on the total protein content as 100%, the contents of α-lactalbumin and β-casein can be controlled in different ranges, wherein the content range of α-lactalbumin is c%-50.5%, and the content range of β-casein is d%-35.0%, and the content ranges of α-lactalbumin and β-casein can be selected separately or in coordination (i.e., the content range of α-lactalbumin is a%-50.5%, and the content range of β-casein is b%-35.0%), wherein c is any value between 13.0-50.5, preferably an integer, i.e., the content range of α-lactalbumin can be 13.0%-50.5%, 15.0%-50.5%, 17.0%-50.5%, 19.0%-50.5%, 21.0%-50.5%, 23.0%-50.5% %, 25.0%-50.5%, 27.0%-50.5%, 29.0%-50.5%, 31.0%-50.5%, 33.0%-50.5%, 35.0%-50.5%, 37.0%-50.5%, 39.0%-50.5%, 41.0%-50.5%, 43.0%-50.5%, 45.0%-50.5%, 47.0%-50.5%, 49.0%-5 0.5%, etc.; d is any value between 15.0-35.0, preferably an integer, that is, the content of β-casein can range from 17.0% to 35.0%, 19.0% to 35.0%, 21.0% to 35.0%, 23.0% to 35.0%, 25.0% to 35.0%, 27.0% to 35.0%, 29.0% to 35.0%, 31.0% to 35.0%, 33.0% to 35.0%, etc.The above-mentioned content ranges of α-lactalbumin and β-casein can be combined or included in the numerical range within the above-mentioned ranges, for example: taking the total protein content of the infant formula milk powder as 100%, the infant formula milk powder contains 15.0%-32.0% of α-lactalbumin and 26.0%-32.0% of β-casein, or the infant formula milk powder contains 15.0%-32.0% of α-lactalbumin and 29.0%-32.0% of β-casein, or Infant formula milk powder contains 16.0%-32.0% alpha-lactalbumin and 20.0%-32.0% beta-casein, or the infant formula milk powder contains 16.0%-32.0% alpha-lactalbumin and 26.0%-32.0% beta-casein, or the infant formula milk powder contains 16.0%-32.0% alpha-lactalbumin and 29.0%-32.0% beta-casein, or the infant formula milk powder contains 21.0%- 32.0% of α-lactalbumin and 20.0%-32.0% of β-casein, or the infant formula milk powder contains 21.0%-32.0% of α-lactalbumin and 26.0%-32.0% of β-casein, or the infant formula milk powder contains 21.0%-32.0% of α-lactalbumin and 29.0%-32.0% of β-casein, or the infant formula milk powder contains 29.0%-32.0% of α-lactalbumin and 26.0%-32.0% of β-casein, The infant formula milk powder contains 20.0%-32.0% of β-casein, or the infant formula milk powder contains 29.0%-32.0% of α-lactalbumin and 26.0%-32.0% of β-casein, or the infant formula milk powder contains 29.0%-32.0% of α-lactalbumin and 29.0%-32.0% of β-casein, or the infant formula milk powder contains 29.0%-32.0% of α-lactalbumin and 32.0% of β-casein. In addition to α-lactalbumin and β-casein, the above-mentioned infant formula milk powder also contains other types of proteins.

[0051] According to a specific embodiment of the present invention, in the above-mentioned infant formula milk powder, the content of whey protein and casein can be controlled in different ranges based on the total protein content of 100%: preferably, the content of whey protein in the infant formula milk powder is controlled to 55.0%-90.0%, and the content of casein is controlled to 10.0%-45.0%; more preferably, the content of whey protein in the infant formula milk powder is controlled to 55.0%-80.0%, and the content of casein is controlled to 20.0%-45.0%; further preferably, the content of whey protein in the infant formula milk powder is controlled to 55.0%-70.0%, and the content of casein is controlled to 30.0%-45.0%; further preferably, the content of whey protein in the infant formula milk powder is controlled to 55.0%-65.0%, and the content of casein is controlled to 35.0%-45.0%. Wherein, the sum of the content of whey protein and casein is not required to be 100%.

[0052] According to a specific embodiment of the present invention, preferably, the total protein content in the infant formula milk powder of the present invention is 10-23g / 100g; the proportion of whey protein in the total protein is 38-70%; the fat content is 15-29g / 100g; the linoleic acid content is 1800-5000mg / 100g; the α-linolenic acid content is 200-500mg / 100g; the dietary fiber content is 0.95-6.3g / 100g (preferably, the dietary fiber includes galacto-oligosaccharides and fructo-oligosaccharides); and the carbohydrate content is 50-58g / 100g.

[0053] According to a specific embodiment of the present invention, preferably, the protein source of the infant formula milk powder of the present invention (i.e., the raw material providing total protein) includes one or a combination of two or more of milk, whole milk powder, skim milk powder, whey protein powder, desalted whey powder, and the protein composition; wherein the protein composition may include the raw material α-lactalbumin powder added to strengthen α-lactalbumin, and the raw material β-casein powder added to strengthen β-casein, or the protein composition of the present invention that can improve the protein digestibility in infant formula milk powder.

[0054] According to a specific embodiment of the present invention, preferably, in the infant formula milk powder of the present invention, based on the total protein content of the milk powder as 100%, the infant formula milk powder contains about 13.0%-50.5% of α-lactalbumin and about 15.0%-35.0% of β-casein. In addition, the proportion of whey protein to total protein is usually controlled to be 40%-70%.

[0055] According to a specific embodiment of the present invention, preferably, based on 1000 parts by weight of infant formula milk powder, its raw materials include: 800-3500 parts by weight of raw cow's milk and 0-400 parts by weight of skimmed milk powder; part or all of the milk and skimmed milk powder can be replaced with whole milk powder or skimmed milk with an equivalent amount of protein.

[0056] According to a specific embodiment of the present invention, preferably, the raw materials of the infant formula milk powder of the present invention also include one or a combination of two or more of whey protein powder (e.g., whey protein powder WPC80%, whey protein powder WPC 34%, etc.) added for fortifying whey protein (i.e., as a component of the protein composition), desalted whey powder (e.g., desalted whey powder D70, D90, etc.), preferably desalted whey powder and / or whey protein powder (e.g., whey protein powder WPC 80% and / or whey protein powder WPC 34%); and raw material α-lactalbumin powder is further added to fortify the α-lactalbumin in the product, and raw material β-casein powder is further added to fortify the β-casein in the product.

[0057] According to a specific embodiment of the present invention, preferably, the protein composition is composed of at least one of the following protein powders and / or milk powders: whey protein powder with an α-lactalbumin content of 25%-95%, whey protein powder with a β-casein content of 35%-80%, whey protein powder with a protein content of 60%-95%, and milk powder (preferably skimmed milk powder) with a protein content of 15%-50%;

[0058] More preferably, the protein composition is composed of at least one of the following protein powders and / or milk powders:

[0059] (1) a whey protein powder having an α-lactalbumin content of 25% to 55%;

[0060] (2) whey protein powder having an α-lactalbumin content of 75% to 95%;

[0061] (3) whey protein powder with a β-casein content of 35% to 70%;

[0062] (4) whey protein powder with a β-casein content of 40% to 80%;

[0063] (5) whey protein powder having a protein content of 60% to 95%;

[0064] (6) Milk powder with a protein content of 15%-50% (preferably skimmed milk powder).

[0065] According to a specific embodiment of the present invention, preferably, based on 1000 parts by weight of infant formula milk powder, its raw materials include: 0-170 parts by weight of whey protein powder, 25-400 parts by weight of desalted whey powder, 0-55 parts by weight of α-lactalbumin powder, and 0-45 parts by weight of β-casein powder.

[0066] According to a specific embodiment of the present invention, preferably, the raw materials providing fat for infant formula milk powder include vegetable oil or OPO structured fat in addition to the basic raw materials containing milk fat (such as the aforementioned raw cow's milk, skimmed milk powder) or anhydrous butter, that is, the source of fat of the infant formula milk powder includes milk fat and / or anhydrous butter; preferably, it also includes vegetable oil and / or OPO structured fat.

[0067] According to a specific embodiment of the present invention, preferably, the vegetable oil used as the raw material for providing fat can include one or more combinations of sunflower oil, corn oil, soybean oil, rapeseed oil, coconut oil, palm oil, and walnut oil, preferably including one or more combinations of sunflower oil, corn oil, and soybean oil. The addition of these vegetable oils provides fat components for the product on the one hand, and linoleic acid on the other hand, and α-linolenic acid can also be provided (preferably, the α-linolenic acid content in the infant formula milk powder of the present invention is 200-500mg / 100g). In addition, the raw material for providing fat can also selectively include the raw material OPO structured fat added to provide 1,3-dioleyl-2-palmitoyl triglyceride. Since the purity of the OPO structural fat raw materials currently sold on the market is different, that is, the content of the active ingredient 1,3-dioleyl-2-palmityl triglyceride therein is different, usually around 40%-70%, in the present invention, in order to distinguish the active ingredient 1,3-dioleyl-2-palmityl triglyceride and its raw materials, the term "1,3-dioleyl-2-palmityl triglyceride" is used when describing the active ingredient, and the commonly known "OPO structural fat" is used when describing the raw materials of infant formula milk powder providing the active ingredient 1,3-dioleyl-2-palmityl triglyceride. The specific addition amount of OPO structural fat can be converted according to the content requirements of 1,3-dioleyl-2-palmityl triglyceride in the infant formula milk powder of the present invention and the purity of the OPO structural fat raw materials. More preferably, based on 1000 parts by weight of infant formula milk powder, its raw materials include: 0-80 parts by weight of sunflower oil; 0-40 parts by weight of corn oil; 0-80 parts by weight of soybean oil; 0-140 parts by weight of OPO structured fat; and 0-4 parts by weight of anhydrous butter.

[0068] According to a specific embodiment of the present invention, preferably, the contents of linoleic acid and α-linolenic acid in the sunflower oil are 7.6-8.9% and 0.25-0.38% respectively; the contents of linoleic acid and α-linolenic acid in the corn oil are 53.0-56.20% and 0.9-1.6% respectively; the contents of linoleic acid and α-linolenic acid in the soybean oil are 50.0-53.5% and 7.6-9.6% respectively; the contents of linoleic acid and α-linolenic acid in the OPO structured lipid are 5.9-6.3% and 0.4-0.62% respectively; the contents of linoleic acid and α-linolenic acid in the low-erucic acid rapeseed oil are 16-19% and 8.0-10.6% respectively; the contents of linoleic acid and α-linolenic acid in the coconut oil are 1-3% and 0-1% respectively; and the effective content of 1,3-dioleyl-2-palmitoyl triglyceride in the raw material of the OPO structured lipid is 40%-70%.

[0069] According to a specific embodiment of the present invention, in the infant formula milk powder of the present invention, a part of the carbohydrates comes from basic raw materials containing lactose such as milk, whole milk powder and / or skim milk powder, etc., and lactose raw materials should be added to provide carbohydrates. That is, in the infant formula milk powder of the present invention, the raw materials for providing carbohydrates include raw material lactose in addition to the basic raw materials containing lactose. Preferably, based on 1000 parts by weight of the infant formula milk powder of the present invention, its raw materials include: 125-550 (preferably 125-325) parts by weight of lactose. The specific addition amount of lactose can be adjusted within the range so that the carbohydrate content of the infant formula milk powder of the present invention is 50-58g / 100g.

[0070] According to a specific embodiment of the present invention, the raw materials of the infant formula milk powder of the present invention may also include one or a combination of two or more of appropriate DHA, ARA, nucleotides, lactoferrin, etc. Preferably, based on 1000 parts by weight of the infant formula milk powder of the present invention, the raw materials include: 2-15 (preferably 8-15) parts by weight of DHA, 3-22 parts by weight of ARA, 0-1.4 parts by weight of lactoferrin, and 0-0.7 parts by weight of nucleotides.

[0071] According to a specific embodiment of the present invention, the raw materials of the infant formula milk powder of the present invention may also include compound nutrients including calcium powder, vitamins and minerals. The compound nutrients are a combination of nutrients that meet national standards, and different addition amounts are used according to different formulas. Preferably, based on 1000 parts by weight of infant formula milk powder, its raw materials include: 7-22 parts by weight of compound nutrients including calcium powder, vitamins and minerals. More preferably, the compound nutrients are added in the form of at least a compound vitamin package, calcium powder, and a mineral nutrient package. Specifically, the compound nutrients are preferably added in the form of the following nutrient packages:

[0072] 1) Compound vitamin nutrient pack, the content of each component in each gram of compound vitamin nutrient pack is as follows:

[0073] Taurine: 140-340mg

[0074] Vitamin A: 1700-5800 μg RE

[0075] Vitamin D: 25-70 μg

[0076] Vitamin B1: 3000-6800 μg

[0077] Vitamin B2: 3500-6900 μg

[0078] Vitamin B6: 2400-4000 μg

[0079] Vitamin B12: 8-20 μg

[0080] Vitamin K1: 200-700 μg

[0081] Vitamin C: 155-700mg

[0082] Vitamin E: 10-70 mg α-TE

[0083] Nicotinamide: 10000-41550 μg

[0084] Folic acid: 500-920 μg

[0085] Biotin: 100-245 μg

[0086] Pantothenic acid: 7100-25230 μg

[0087] Inositol: 0-250mg

[0088] L-Carnitine: 0-60mg

[0089] 2) Mineral 2 Nutrition Pack, the content of each component in each gram of Mineral 2 Nutrition Pack is as follows:

[0090] Calcium: 300-455mg

[0091] Phosphorus: 75-150 mg

[0092] 3) Mineral nutrition pack, the content of each component in each gram of mineral nutrition pack is as follows:

[0093] Iron: 40-110 mg

[0094] Zinc: 23-90 mg

[0095] Copper: 2600-4180 μg

[0096] Iodine: 500-995 μg

[0097] Selenium: 0-200 μg

[0098] Manganese: 0-579 μg

[0099] 4) Compound magnesium chloride nutrient pack, each gram of compound magnesium chloride nutrient pack contains:

[0100] Magnesium: 80-170mg

[0101] 5) Compound potassium chloride nutrient pack, each gram of compound potassium chloride nutrient pack contains:

[0102] Potassium: 400-580 mg;

[0103] 6) Choline chloride nutrient pack, each gram of choline chloride nutrient pack contains:

[0104] Choline: 300-950 mg;

[0105] Preferably, based on 1000 parts by weight of infant formula milk powder, the added amount of the compound vitamin nutrient package is 2-4 parts by weight, the added amount of the mineral 2 nutrient package is 2-12 parts by weight, the added amount of the mineral 1 nutrient package is 0.5-3 parts by weight, the added amount of the compound magnesium chloride nutrient package is 0-2 parts by weight, the added amount of the compound potassium chloride nutrient package is 0-4.5 parts by weight, and the added amount of choline chloride nutrient is 0-2 parts by weight, and the base material of each nutrient package is preferably lactose or sodium L-ascorbate.

[0106] The content of each component of the above-mentioned compound nutrients refers to the amount added to strengthen the nutrient substance, and does not include the content of nutrient components in other raw materials of milk powder. For example, the calcium powder (calcium carbonate) in Mineral II, "Calcium: 1300-1600g" in every 1000kg of milk powder means to strengthen the calcium element in the product. Based on 1000kg of milk powder, the added mineral II (such as calcium carbonate) nutrient package contains 1300-1600g of calcium by weight.

[0107] According to a specific embodiment of the present invention, the raw materials of the infant formula milk powder of the present invention may further include probiotics, preferably, the probiotics are bifidobacteria. More preferably, based on 1000 parts by weight of infant formula milk powder, the amount of bifidobacteria added is 0.1-0.2 parts by weight; more preferably, 0.18-0.2 parts by weight. More preferably, each part by weight of bifidobacteria powder contains 3×10 10 CFU or above.

[0108] According to a specific embodiment of the present invention, the raw materials of the infant formula milk powder of the present invention preferably include:

[0109]

[0110] 7-22 parts by weight of a compound nutrient comprising calcium powder, vitamins and minerals;

[0111]

[0112]

[0113] It is understood that the specific amount of each raw material in the infant formula milk powder of the present invention should be adjusted and determined on the premise of meeting the product index requirements of the infant formula milk powder. In the infant formula milk powder of the present invention, the product performance indicators not described or listed in detail should be implemented in accordance with the national standards for infant formula milk powder or reconstituted milk powder and the provisions of relevant standards and regulations.

[0114] In the infant formula milk powder of the present invention, each raw material can be commercially available, and the selection of each raw material should comply with the requirements of relevant standards, wherein the protein composition should meet the requirements of the present invention. In addition, the compounded nutrients can also be compounded by themselves. The present invention only uses "compounding" for the convenience of expression, and does not mean that the components in the compound must be mixed together before use. Each raw material should be added and used under the premise of meeting relevant laws and regulations.

[0115] According to a specific embodiment of the present invention, preferably, the infant formula milk powder of the present invention is a formula milk powder suitable for infants and children aged 0-7 years old, and the protein digestion and absorption of the formula milk powder is better.

[0116] On the other hand, the present invention also provides a method for preparing the above-mentioned infant formula milk powder, and the process flow of the preparation mainly includes: batching, homogenization, concentration and sterilization, spray drying, and dry mixing to obtain the finished product. Specifically, the preparation method of the infant formula milk powder of the present invention includes:

[0117] The milk, powder raw materials and melted oil raw materials that have been coarsely filtered, homogenized and sterilized are mixed, and compound nutrients are added to obtain a mixed liquid;

[0118] The mixed liquid is filtered, homogenized, cooled, concentrated and sterilized, spray-dried, and fluidized bed dried and cooled to obtain dry milk powder, which is mixed with DHA, ARA, lactoferrin, nucleotides, and bifidobacteria, and sieved to obtain the infant formula milk powder.

[0119] In the above preparation method, preferably, the first-level pressure of the homogenization treatment of the mixed liquid is 105±5 bar, and the second-level pressure is 32±3 bar.

[0120] In the above preparation method, preferably, the concentration and sterilization adopts double-effect concentration, more preferably, the sterilization temperature is ≥83°C, and the sterilization time is 25 seconds; further preferably, the discharge concentration is 48%-52% dry matter.

[0121] In the above preparation method, preferably, the inlet air temperature of the spray drying is 165-180°C, the exhaust air temperature is 75-90°C, the high-pressure pump pressure is 160-210 bar, and the tower negative pressure is -4 mbar to -2 mbar.

[0122] In the above preparation method, preferably, the fluidized bed drying and cooling includes two drying and cooling steps, and the temperature of the milk powder after the second drying and cooling step is 25-30°C; at the same time, the phospholipids and the carrier are mixed and heated to 60-65°C, and evenly dispersed on the surface of the milk powder under the action of compressed air.

[0123] According to a specific embodiment of the present invention, the preparation process of the infant formula milk powder of the present invention may include the following specific steps:

[0124] 1) Milk coarse filtration: After the milk is coarsely filtered and degassed in the balance cylinder, it is preheated by a plate heat exchanger and then separated from impurities by a separator.

[0125] 2) Milk homogenization and sterilization: After removing impurities, part of the milk enters the homogenizer for homogenization, and the other part is not homogenized. The homogenized milk and the unhomogenized milk are mixed and then enter the sterilization system for sterilization. After sterilization, they enter the mixing tank.

[0126] 3) Powder addition: Various powder raw materials are measured according to the formula and added into the powder mixing tank through the air delivery system for storage.

[0127] 4) Vacuum powder suction: Various powder raw materials in the powder mixing tank are sucked into the mixing tank through the vacuum system.

[0128] 5) Melting and oil preparation: Put the grease specified in the formula into the carburettor room according to the formula requirements. The temperature in the carburettor room should be maintained at 50-90℃. After the oil is dissolved, it is pumped into the mixed oil storage tank through the oil pump and flow meter according to the formula ratio requirements.

[0129] 6) Storage of mixed oil: The mixed oil is stored in an oil storage tank at a temperature of 40-50°C for less than 12 hours to prevent fat oxidation.

[0130] 7) Weighing: Pump the mixed oil into the mixing tank through the oil pump according to the formula requirements.

[0131] 8) Dissolved and added nutrients: Calcium powder, minerals, vitamins, etc. are added separately, dissolved in 100-200kg of pure water, and then poured into the mixing tank. After each one is added, rinse the addition tank and pipeline with 100kg of pure water.

[0132] 9) Filtration: Mix the various raw materials in a mixing tank, and filter the mixed liquid through a filter to remove physical impurities that may be introduced into the raw materials.

[0133] 10) Homogenization: The mixed liquid is homogenized by a homogenizer with a first-stage pressure of 105±5 bar and a second-stage pressure of 32±3 bar. The fat globules are mechanically treated and dispersed into uniform fat globules.

[0134] 11) Cooling and storage: The homogenized liquid enters the plate heat exchanger for cooling, and is cooled to below 20°C. It is temporarily stored in the pre-storage tank and enters the next process within 6 hours. The agitator is turned on according to the set requirements.

[0135] 12) Concentration and sterilization: Double-effect concentration is used during production, the sterilization temperature is ≥83°C, and the sterilization time is 25 seconds; the discharge concentration is 48%-52% dry matter.

[0136] 13) Concentrated milk storage, preheating filtration, spray drying: The concentrated milk is temporarily stored in a concentrated milk balance tank; it is preheated to 60-70°C in a scraper preheater, and the preheated material is filtered through a filter with a pore size of 1mm, and then pumped into a drying tower for spray drying with a high-pressure pump. The fine powder is agglomerated at the top of the tower or in a fluidized bed as required; wherein, the inlet air temperature is 165-180°C, the exhaust air temperature is 75-90°C, the high-pressure pump pressure is 160-210bar, and the tower negative pressure is -4mbar to -2mbar.

[0137] 14) Fluidized bed drying and cooling: The milk powder coming out of the drying tower is dried twice in a fluidized bed (primary stage) and then cooled to 25-30°C in a fluidized bed (secondary stage); at the same time, the lecithin is mixed with the carrier and heated to 60-65°C. Under the action of compressed air, the lecithin is evenly dispersed on the surface of the milk powder, so that the powder particles agglomerate to increase their particle size and solubility.

[0138] 15) Packaging: According to the formula requirements, DHA, ARA, lactoferrin, nucleotides, and bifidobacteria are weighed and sealed in bags for packaging.

[0139] 16) Dry mixing: Mix the weighed DHA, ARA, lactoferrin, nucleotides, bifidobacteria and milk powder in a dry mixer.

[0140] 17) Powder screening: Use a vibrating screen to make the particle size of the milk powder uniform and discard the powder residue.

[0141] 18) Powder output: Collect the powder in a sterilized powder box and transport it from the powder output room to the powder loading room.

[0142] 19) Powdering: Pour the milk powder into the powder storage tank on the large and small packaging machines according to the packaging requirements.

[0143] 20) Packaging: 800g automatic packaging machine nitrogen filling packaging; the oxygen content during nitrogen filling is less than 1%; 900g iron cans automatically filled with nitrogen packaging, the oxygen content is less than 5%.

[0144] 21) Packing: Put the packaged sachets into cartons and add the powder spoon, then seal with a carton sealing machine.

[0145] 22) Finished product inspection: Sampling inspection is carried out on the packaged products according to the inspection plan.

[0146] 23) Storage: Products that have passed the inspection shall be stored in warehouses at room temperature with humidity ≤ 65%.

[0147] In summary, the infant formula milk powder provided by the present invention has the effect of promoting protein digestion and absorption.

[0148] The technical solution of the present invention has the following beneficial effects:

[0149] (1) The technical solution of the present invention can significantly improve the digestibility of whey protein in infant formula milk powder.

[0150] (2) The technical solution of the present invention can release more types of free amino acids.

[0151] (3) The technical solution of the present invention can increase the release ratio of the essential amino acid EAA.

[0152] (4) The technical solution of the present invention can produce more small molecule peptides during the digestion process.

[0153] (5) The technical solution of the present invention can produce more characteristic peptide segments. BRIEF DESCRIPTION OF THE DRAWINGS

[0154] Figure 1-Figure 6 The results of releasing small molecule peptides from the samples of the protein compositions of Examples 1-5 and Comparative Example 1 in an in vitro gastric digestion experiment. DETAILED DESCRIPTION

[0155] The following detailed description of the method for preparing infant formula milk powder of the present invention is provided through specific examples, which is intended to help readers better understand the essence and features of the present invention, and is not intended to limit the scope of implementation of the present invention. The methods in the following examples without specifying specific conditions are generally carried out according to conventional operations in the relevant field.

[0156] Raw materials description of the embodiments and comparative examples:

[0157] The skimmed milk powder is produced by Fonterra Group and has a protein content of 33.5%.

[0158] WPC80 is concentrated whey protein powder with a protein content of 80%, purchased from Wheyco Company of Germany.

[0159] The whey protein powder with low α-lactalbumin content had an α-lactalbumin content of 41% and was purchased from Arla Corporation of Denmark.

[0160] The whey protein powder with high α-lactalbumin content has an α-lactalbumin content of 92.3%, and is purchased from Angopur Company of the United States.

[0161] The whey protein powder with low β-casein content had a β-casein content of 53% and was purchased from Kerry, Ireland.

[0162] The whey protein powder with high β-casein content had a β-casein content of 63.2% and was purchased from Arla Corporation of Denmark.

[0163] Lactose was purchased from Glanbia, USA.

[0164] Example 1

[0165] This example provides a protein composition (sample 1), the raw material composition of which includes (by weight):

[0166]

[0167] The protein composition provided in this embodiment has a whey protein content of 60% and a casein content of 40% (based on the total protein content of 100%). The total protein content, the content and proportion of α-lactalbumin, the content and proportion of β-casein, etc. are shown in Table 1.

[0168] Example 2

[0169] This example provides a protein composition (sample 2), the raw material composition of which includes (by weight):

[0170]

[0171] The protein composition provided in this embodiment has a whey protein content of 60% and a casein content of 40% (based on the total protein content of 100%). The total protein content, the content and proportion of α-lactalbumin, the content and proportion of β-casein, etc. are shown in Table 1.

[0172] Example 3

[0173] This example provides a protein composition (sample 3), the raw material composition of which includes (by weight):

[0174]

[0175] The protein composition provided in this embodiment has a whey protein content of 60% and a casein content of 40% (based on the total protein content of 100%). The total protein content, the content and proportion of α-lactalbumin, the content and proportion of β-casein, etc. are shown in Table 1.

[0176] Example 4

[0177] This example provides a protein composition (sample 4), the raw material composition of which includes (by weight):

[0178]

[0179] The protein composition provided in this embodiment has a whey protein content of 60% and a casein content of 40% (based on the total protein content of 100%). The total protein content, the content and proportion of α-lactalbumin, the content and proportion of β-casein, etc. are shown in Table 1.

[0180] Example 5

[0181] This example provides a protein composition (sample 5), the raw material composition of which includes (by weight):

[0182]

[0183] The protein composition provided in this embodiment has a whey protein content of 60% and a casein content of 40% (based on the total protein content of 100%). The total protein content, the content and proportion of α-lactalbumin, the content and proportion of β-casein, etc. are shown in Table 1.

[0184] Comparative Example 1

[0185] This comparative example provides a protein composition (sample 6), the raw material composition of which includes (by weight):

[0186] 160 servings of skimmed milk powder

[0187] WPC80 70 parts

[0188] Lactose 450 parts.

[0189] The protein composition provided in this comparative example has a whey protein content of 60% and a casein content of 40% (based on the total protein content of 100%). The total protein content, the content and proportion of α-lactalbumin, the content and proportion of β-casein, etc. are shown in Table 1.

[0190] Example 6

[0191] This example provides a protein composition (sample 7), the raw material composition of which includes (by weight):

[0192] 230 servings of skimmed milk powder

[0193] 16.5 servings of whey protein powder with high α-lactalbumin content

[0194] Lactose 300 parts.

[0195] The protein composition provided in this embodiment has a whey protein content of 35% and a casein content of 65% (based on the total protein content of 100%). The total protein content, the content and proportion of α-lactalbumin, the content and proportion of β-casein, etc. are shown in Table 1.

[0196] Example 7

[0197] This example provides a protein composition (sample 8), the raw material composition of which includes (by weight):

[0198]

[0199] The protein composition provided in this embodiment has a whey protein content of 35% and a casein content of 65% (based on the total protein content of 100%). The total protein content, the content and proportion of α-lactalbumin, the content and proportion of β-casein, etc. are shown in Table 1.

[0200] Comparative Example 2

[0201] This comparative example provides a protein composition (sample 9), the components of which include (by weight):

[0202] 262 parts skim milk powder

[0203] WPC80 26 parts

[0204] Lactose 383.5 parts.

[0205] The protein composition provided in this comparative example has a whey protein content of 35% and a casein content of 65% (based on the total protein content of 100%). The total protein content, the content and proportion of α-lactalbumin, the content and proportion of β-casein, etc. are shown in Table 1.

[0206] Example 8

[0207] This embodiment provides a protein composition (sample 10), the raw material composition of which includes (by weight):

[0208]

[0209] The protein composition provided in this embodiment has a whey protein content of 50% and a casein content of 50% (based on the total protein content of 100%). The total protein content, the content and proportion of α-lactalbumin, the content and proportion of β-casein, etc. are shown in Table 1.

[0210] Example 9

[0211] This example provides a protein composition (sample 11), the raw material composition of which includes (by weight):

[0212]

[0213] The protein composition provided in this embodiment has a whey protein content of 50% and a casein content of 50% (based on the total protein content of 100%). The total protein content, the content and proportion of α-lactalbumin, the content and proportion of β-casein, etc. are shown in Table 1.

[0214] Example 10

[0215] This example provides a protein composition (sample 12), the raw material composition of which includes (by weight):

[0216]

[0217]

[0218] The protein composition provided in this embodiment has a whey protein content of 50% and a casein content of 50% (based on the total protein content of 100%). The total protein content, the content and proportion of α-lactalbumin, the content and proportion of β-casein, etc. are shown in Table 1.

[0219] Comparative Example 3

[0220] This comparative example provides a protein composition (sample 13), the raw material composition of which includes (by weight):

[0221] 202 parts skim milk powder

[0222] WPC80 52 parts

[0223] Lactose 418 parts.

[0224] The protein composition provided in this comparative example has a whey protein content of 50% and a casein content of 50% (based on a total protein content of 100%). The total protein content, the content and proportion of α-lactalbumin, the content and proportion of β-casein, etc. are shown in Table 1.

[0225] Embodiment 11

[0226] This example provides a protein composition (sample 14), the raw material composition of which includes (by weight):

[0227]

[0228] The protein composition provided in this embodiment has a whey protein content of 70% and a casein content of 30% (based on the total protein content of 100%). The total protein content, the content and proportion of α-lactalbumin, the content and proportion of β-casein, etc. are shown in Table 1.

[0229] Example 12

[0230] This example provides a protein composition (sample 15), the raw material composition of which includes (by weight):

[0231]

[0232]

[0233] The protein composition provided in this embodiment has a whey protein content of 70% and a casein content of 30% (based on the total protein content of 100%). The total protein content, the content and proportion of α-lactalbumin, the content and proportion of β-casein, etc. are shown in Table 1.

[0234] Comparative Example 4

[0235] This comparative example provides a protein composition (sample 16), the raw material composition of which includes (by weight):

[0236] 121 servings of skimmed milk powder

[0237] WPC80 86.5 parts

[0238] Lactose 525 parts.

[0239] The protein composition provided in this comparative example has a whey protein content of 70% and a casein content of 30% (based on the total protein content of 100%). The total protein content, the content and proportion of α-lactalbumin, the content and proportion of β-casein, etc. are shown in Table 1.

[0240] Table 1

[0241]

[0242]

[0243] The following is an in vitro simulation of the digestion process of the infant stomach and small intestine to evaluate the protein digestion of the samples provided in Examples 1-12 and Comparative Examples 1-4, including the degradation of whey protein in the stomach, the release of amino acids after small intestinal digestion, and the production effect of peptides.

[0244] 1. In vitro gastric digestion experiment

[0245] 1. Sample Preparation and Processing

[0246] The method of in vitro simulated gastric digestion experiment of samples was based on the Dupont method with some modifications, as follows:

[0247] (1) Preparation of milk sample: According to the protein content determination results, the sample concentration was adjusted to 10 mg / mL with deionized water to prepare 50 mL of milk sample, and then 1 mol / L HCl was added to adjust the pH value to 4.0, and preheated in a 37°C water bath.

[0248] (2) Preparation of simulated gastric fluid: Weigh 4.33 mg of pepsin and 23.755 mg of gastric lipase, add 45 mL of 0.15 M NaCl solution, adjust the pH to 4.0 with 1 M hydrochloric acid, and make the volume to 50 mL to prepare simulated gastric fluid, so that the concentration of pepsin in the system is 113.75 U / mL and the final concentration of gastric lipase is 21 U / mL.

[0249] (3) Gastric digestion experimental process: 50 mL of milk sample was added to 50 mL of simulated gastric fluid in an enzyme reactor (37°C). Samples were taken at 0 h and 3 h of digestion, with 20 mL sampled each time. The pH of the samples was adjusted to 7.0 with 1 M NaOH to terminate the reaction.

[0250] The samples of Examples 1-12 and Comparative Examples 1-4 were pre-treated in the above manner to obtain gastric digestive juice samples at different digestion times.

[0251] 2. Semi-quantitative analysis of digestive juice

[0252] (1)SDS-PAGE:

[0253] The samples obtained at 0h and 3h were diluted with deionized water in a ratio of 1:1, mixed with 2× sample buffer (premixed protein sample buffer from Bio-rad) in a ratio of 1:1, and then heated to boil. The sample loading volume was 5 μL.

[0254] The following electrophoresis and staining methods were used:

[0255] The concentration of the separation gel is 12% (w / v), and the concentrated gel is 5% (w / v). The specific formula is shown in Table 2; the electrophoresis process is constant voltage, the concentrated gel voltage is 150V, and the separation gel voltage is 300V; the staining agent is 0.1% Coomassie Brilliant Blue R-250 solution, and the decolorizing agent is a mixed solution prepared by ethanol and acetic acid; after the gel run is completed, the gel is gently removed, the electrophoresis buffer is washed away, and then it is stained with Coomassie Brilliant Blue staining solution for 2h on a horizontal shaker, and then decolorized with a decolorizing solution until the bands are clearly visible.

[0256] Table 2 Gel composition of SDS-PAGE

[0257] Element 12% separation gel (mL) 5% concentrated gel (mL) Distilled water 10.2 5.8 30% Acr-Bis (29:1) 12.0 1.7 Separation gel buffer (4X) 7.5 - Stacking gel buffer (4X) - 2.5 10% Gel Polymerization Catalyst 0.3 0.1 TEMED 0.012 0.01

[0258] (2) Semi-quantitative analysis of protein content changes: Tanon Image electrophoresis image processing software was used to perform a semi-quantitative analysis of the trend of changes in the relative content of milk protein on the electrophoretic bands of the gastric digestive fluid samples of Examples 1-5 and Comparative Example 1. The relative content of whey protein at 0 min was set to 100%, and the relative content of protein at other time points was the ratio of the band area at that time point to that at 0 min.

[0259] 2. Experiments on gastric and intestinal digestion in vitro

[0260] 1. Sample Preparation and Processing

[0261] (1) Preparation of milk sample: adjust the sample concentration to 10 mg / mL with deionized water to prepare 50 ml of milk sample, then add 1 mol / L HCl to adjust the pH value to 4.0, and preheat in a 37°C water bath.

[0262] (2) Preparation of simulated gastric fluid: Weigh 4.33 mg of pepsin and 23.755 mg of gastric lipase, add 45 mL of 0.15 M NaCl solution, adjust the pH to 4.0 with 1 M hydrochloric acid, and make the volume to 50 mL to prepare simulated gastric fluid, so that the concentration of pepsin in the system is 113.75 U / mL and the final concentration of gastric lipase is 21 U / mL.

[0263] (3) Preparation of simulated intestinal fluid: Weigh 0.324 mg trypsin, 0.988 mg chymotrypsin, 2.27 g pancreatic lipase and 0.66 g bile salt, add 245 mL 0.15 M NaCl solution, adjust the pH to 6.5 with 1 M NaOH, and make up to 250 mL to prepare simulated intestinal fluid.

[0264] (4) Gastrointestinal digestion experimental process: 25 mL of milk sample was added to 25 mL of simulated gastric fluid in an enzyme reactor (37°C) for 2 h of gastric digestion, and then digested in simulated intestinal fluid. Samples were taken at 0, 2 h in the stomach, 0.25 h in the intestine, 1 h in the intestine, and 2 h in the intestine, respectively. 20 mL was sampled each time. The sample was terminated with an enzyme preparation to obtain different digestive fluid samples. Taking "1 h in the intestine" as an example, it means that the sample was digested in simulated gastric fluid for 2 h and then digested in simulated intestinal fluid for another 1 h.

[0265] 2. Determination of free amino acids

[0266] Take 1 mL of digestion sample at each time point and dilute it with an equal volume of 10 g / 100 mL TCA, shake well and ultrasonicate for 30 min, let it stand for more than 2 h; centrifuge the sample at 10,000 rpm for 30 min; take the supernatant and pass it through a 0.45 μm membrane, then take 400 μL of the supernatant and put it into a liquid phase vial.

[0267] The amino acid content of the samples was analyzed by HPLC using a high-phase liquid chromatography system equipped with a sodium cation exchange amino acid analysis column (4×150 mm, Pickering, USA) and a phthaloyl post-column derivatization system (Pickering, USA).

[0268] Chromatographic conditions: Angilent Hypersil ODS column (5μm, 4.0mm×250mm); column temperature was 40℃; mobile phase A (pH=7.2): 27.6mmol / L sodium acetate-triethylamine-tetrahydrofuran (volume ratio of 500:0.11:2.5), mobile phase B (pH=7.2): 80.9mmol / L sodium acetate-methanol-acetonitrile (volume ratio of 1:2:2); gradient elution was used, and the elution program was: 0min, 8% B; 17min, 50% B; 20.1min, 100% B; 24.0min, 0% B; mobile phase flow rate was 1.0mL / min; the detection wavelength of the ultraviolet detector (VWD) was 338nm, and proline was detected at 262nm; the amino acid content was quantified by the external standard method.

[0269] 3. Determination of Peptide Fragments after Digestion

[0270] The ultrafiltration tube was first centrifuged at 5000g for 20 minutes with PBS buffer, and this step was repeated three times to achieve the purpose of cleaning; the collection tube was replaced, the sample was loaded into the ultrafiltration tube, and the sample was centrifuged at 5000g for 20 minutes to collect the flow-through liquid; the ultrafiltration tube was then washed with 2-3mL of PBS buffer and combined with the flow-through liquid in the third step and then dried; the peptide fragments after drying were desalted using a suitable desalting column, and the obtained sample was collected using Orbitrap Fusion TM Tribrid TM Detection was performed using a three-in-one mass spectrometer.

[0271] (1) HPLC The peptide samples were reconstituted with mobile phase A (2% ACN, 0.1% FA), centrifuged at 20,000g for 10 minutes, and the supernatant was injected. The samples were separated by Thermo UltiMate 3000UHPLC. The samples were first enriched and desalted in a trap column, and then connected in series with a self-assembled C18 column (75um inner diameter, 3μm column material> particle size, 25cm column length) and separated at a flow rate of 300nl / min through the following effective gradient: 0-5min, 5% mobile phase B (98% ACN, 0.1% FA); 5-45min, mobile phase B linearly increased from 5% to 25%; 45-50min, mobile phase B increased from 25% to 35%; 50-52min, mobile phase B increased from 35% to 80%; 52-54min, 80% mobile phase B; 54-60min, 5% mobile phase B. The end of the nanoliter liquid phase separation is directly connected to the mass spectrometer.

[0272] (2) Mass spectrometry detection The peptides separated by liquid phase were ionized by nanoESI source and then entered into the tandem mass spectrometer Q-Exactive HF X (Thermo Fisher Scientific, San Jose, CA) for DDA (data-dependent acquisition) mode detection. The main parameter settings were: ion source voltage was set to 2 kV; primary mass spectrometry scanning range was 350-1500 m / z; resolution was set to 60000; secondary mass spectrometry starting m / z was fixed to 100; resolution was 15000. The screening conditions for the parent ions of the secondary fragmentation were: charge 2+ to 6+, peak intensity exceeding 10,000 and ranked in the top 30 parent ions. The ion fragmentation mode was HCD, and the fragment ions were detected in the Orbitrap. The dynamic exclusion time was set to 30 s. The AGC settings were: primary 3E6, secondary 1E5.

[0273] (3) Data information analysis The data from the machine were identified using the Andromeda engine integrated with MaxQuant. Then, MaxQuant performed quantitative analysis based on the peptide peak intensity, peak area, liquid chromatography retention time and other information related to the primary mass spectrometry, and performed a series of statistical analysis and quality control. The spectral level was filtered with PSM-level FDR <= 1%, and the peptide level was further filtered with Peptide-level FDR <= 1% to obtain significant identification results. Then, based on the identification results, the precursor protein was annotated with GO, COG, and Pathway functions. Based on the quantitative results, the differential peptides between different comparison groups were calculated, and finally the precursor proteins corresponding to the differentially enriched peptides were functionally analyzed.

[0274] Experimental results and analysis:

[0275] 1. Whey protein digestibility

[0276] The whey protein digestion performance data of the in vitro gastric digestion experiments of the samples of Examples 1-7, 9-10, 12 and Comparative Examples 1-4 are shown in Tables 3 and Figure 1 shown.

[0277] Table 3

[0278]

[0279]

[0280] It can be seen from the contents of Table 3 that in the in vitro gastric digestion process, compared with the samples of Comparative Examples 1-4, the samples of Examples 1-7, 9-10, and 12 of the present invention have relatively low proportions of residual whey protein at different digestion time points, which indicates that the digestion of whey protein in the in vitro stomach of Samples 1-5, 7-8, 11-12, and 15 is increased, that is, the protein compositions of Examples 1-7, 9-10, and 12 have better whey protein digestion performance than Comparative Example 1, and the effects of the samples corresponding to Examples 5 and 6 are particularly prominent.

[0281] 2. Free amino acids

[0282] The types of free amino acids released by the samples of the protein compositions of Examples 1-12 and Comparative Examples 1-4 in the in vitro gastric digestion experiment include Lys, Phe, Met, Trp, Thr, Ile, Leu, Val, His, Cys-s, Tyr, Asp, Glu, Ser, Gly, Arg, Ala, Pro, EAA, NEAA, TAA, etc., among which Lys, Phe, Met, Trp, Thr, Ile, Leu, Val, His, and EAA are essential amino acids.

[0283] 3. Free essential amino acids EAA

[0284] The experimental data of free essential amino acid EAA released by the samples of Examples 1-12 and Comparative Examples 1-4 in the in vitro gastrointestinal digestion experiment, that is, the percentage of free essential amino acid EAA in all free amino acids is shown in Table 4. Wherein:

[0285] EAA% = free essential amino acids / total free amino acids × 100%

[0286] Increase factor of EAA% after digestion = (EAA% after digestion - EAA% before digestion) ÷ EAA% before digestion

[0287] Table 4

[0288]

[0289]

[0290] As can be seen from the data in Table 4: in the digestive juice obtained by in vitro gastrointestinal digestion, compared with the corresponding Comparative Examples 1-4, the multiple of the release increase of essential amino acid EAA after digestion of the protein compositions of Examples 1-12 is greater, which indicates that the technical solution provided by the present invention can generate more beneficial essential amino acids during digestion.

[0291] 4. Small molecule peptides (short peptides)

[0292] The proportion data of small molecule peptides released by the protein composition samples of Examples 1-5 and Comparative Example 1 in the in vitro gastrointestinal digestion experiment are shown in Table 5 and Figure 1-Figure 6 as shown, wherein, Figure 1-Figure 5 correspond to the samples of Examples 1-5 respectively, Figure 6 corresponds to the sample of Comparative Example 1 as a control. Among them, n-0 represents the data when sample n is not digested, Gn-120 represents the data when sample n is digested in simulated gastric juice for 120 min, In-120 represents the data when sample n is first digested in simulated gastric juice for 120 min and then digested in simulated intestinal juice for 120 min, and n is 1, 2, 3, 4, 5, 6. Sample n refers to sample 1, sample 2, sample 3, sample 4, sample 5, and sample 6.

[0293] Table 5 (unit: increased percentage (%))

[0294]

[0295] From the data in Table 5 and the Figure 1-Figure 6 curve, it can be seen that: after in vitro gastrointestinal digestion, the increase ratio of small molecule peptides (0 < X ≤ 10) of the protein composition samples of Examples 1-5 is significantly higher than that of the control sample (sample 6 of Comparative Example 1). Generally, it is considered that the process of protein degradation and differentiation into small molecules in the digestive tract. The lower the molecular weight of the peptide segment, the more beneficial it is generally considered for absorption. From this point, it can also prove that the protein compositions of Examples 1-5 have good digestion performance. In particular, the proportion of small peptide segments of the samples corresponding to Examples 2-5 is significantly higher than that of Comparative Example 1, which indicates that when the content of α-lactalbumin is controlled at 21%-32% and the content of β-casein is controlled at 26%-32%, excellent results can be obtained in the release of small molecule peptides.

[0296] 5. Characteristic peptide segments

[0297] The data of characteristic peptide segments released by the protein composition samples of Examples 1-5 in the in vitro gastrointestinal digestion experiment are shown in Table 6.

[0298] Table 6

[0299] Example 1 Example 2 Example 3 Example 4 Example 5 Sample 1 Sample 2 Sample 3 Sample 4 Sample 5 F.QINNKIW.C 2.05E+06 3.24E+06 4.75E+05 1.27E+07 7.57E+06 A.IVENNESTEYGLF.Q 4.99E+06 2.19E+06 2.82E+06 7.76E+06 2.02E+06 K.GYGGVS.L 2.37E+06 4.07E+06 5.64E+06 4.49E+06 5.80E+06 K.FLDDLTDD.I 1.14E+06 6.07E+05 4.66E+05 2.46E+06 1.19E+06 V.MFPPQ.S 2.27E+08 6.24E+08 9.43E+08 9.62E+08 7.86E+08 L.TQTPVVVPP.F 1.17E+08 2.48E+08 3.39E+08 3.90E+08 3.49E+08 L.VYPFPGPIP.N 6.41E+07 1.32E+08 1.43E+08 1.36E+08 1.81E+08 L.PQNIPPL.T 1.18E+08 1.78E+08 1.90E+08 1.21E+08 1.29E+08 Q.TLALPPQP.L 1.58E+07 3.07E+07 4.38E+06 1.02E+08 8.58E+07

[0300] Casein is rapidly hydrolyzed in the stomach into polypeptide fragments, and more peptides are released in the small intestine. Peptides derived from α-lactalbumin and β-lactoglobulin have also been reported many times to be found in the digestive tract. Further studies have shown that these peptides released from milk protein have important physiological functions, such as regulating immune function, lowering blood pressure, inhibiting bacteria, promoting mineral absorption, regulating blood sugar metabolism, regulating appetite, opioid peptides with morphine-like functions, and anti-oxidation. According to reports by Alice et al., after ingesting milk products, multiple peptides with different physiological functions were found in human body fluids, such as opioid peptides derived from β-casein, ACE inhibitory peptides, and ACE inhibitory peptides derived from α-lactalbumin. Karima et al. used LC-MS / MS analysis to show that 30 minutes, 90 minutes and 120 minutes after suckling pigs ingested milk formula powder, a large number of peptides derived from β-casein (positions 74-91) were detected in the jejunum and ileum, respectively. These peptides have immune regulation and blood pressure lowering functions; 30 minutes and 90 minutes after ingestion, 7-15 amino acid peptides derived from β-lactoglobulin were detected in the jejunum, and large peptides derived from β-lactoglobulin were found in the ileum, containing 23 amino acids and 40 amino acids, respectively. These peptides are related to the proliferation of spleen cells and cytokine release, and are speculated to have immune regulation functions.

[0301] Through breast milk research, it was found that there are a large number of peptides in breast milk, and in the infant's digestive tract, more peptides are released as the digestion progresses. In vitro digestion studies have shown that β-casein in breast milk is the main source of functional peptides. 305 peptides from this source were detected in undigested breast milk, and the number of peptides increased to 646 after digestion; for α-lactalbumin, there is a similar phenomenon. No reports of peptides from this source were found in undigested breast milk, but 58 peptides were reported after digestion.

[0302] By comparing the peptides produced after in vitro gastric digestion, it was found that the samples of the protein composition of Examples 1-5 produced some characteristic peptides, which were not found in Sample 6 (Comparative Example 1) used as a control. Further analysis of the functions of these peptides showed that the characteristic peptides produced after digestion are believed to play the following physiological functions in vivo: anti-inflammatory response, resistance to invasion of external pathogens, signal transmission in vivo, etc.

[0303] From the above experimental results, we can see that:

[0304] The proportions of residual whey protein in the samples of Examples 1-7, 9-10, and 12 of the present invention at different digestion time points are relatively low. The protein compositions of these examples have excellent whey protein digestion performance, especially Examples 5 and 6 are the most prominent.

[0305] The samples of the protein compositions of Examples 1-12 can release a variety of free amino acids in in vitro gastric digestion experiments.

[0306] In particular, the protein compositions of Examples 1-12 can release a large amount of essential amino acids after digestion, and the increase multiples of EAA release are relatively large, indicating that the protein compositions of Examples 1-12 can produce more beneficial essential amino acids during digestion.

[0307] After in vitro gastrointestinal digestion, the increase proportions of small-molecule peptides (0 < X ≤ 10) in the samples of the protein compositions of Examples 1-5 are significantly increased, which is significantly better than the control sample (Sample 6 of Comparative Example 1). From this point, it can also be proved that the protein compositions of Examples 1-5 have good digestion performance. In particular, the proportion of small-molecule peptides in the samples corresponding to Examples 2-5 is significantly higher than that of Comparative Example 1, indicating that excellent results can be obtained in the release of small-molecule peptides when the content of α-lactalbumin is controlled at 21%-32% and the content of β-casein is controlled at 26%-32%.

[0308] The samples of the protein compositions of Examples 1-5 can produce characteristic peptide segments during digestion, which are not found in Sample 6 (Comparative Example 1) used as a control. Further analysis of the functions of these peptide segments shows that the characteristic peptides produced after digestion are considered to play the following physiological functions in the body: anti-inflammatory response, resistance to invasion by external pathogens, signal transduction in the body, etc.

[0309] Example 13

[0310] This example provides an infant formula milk powder, which contains a protein composition that can improve the protein digestion performance in infant formula milk powder. Based on the total weight of 1000 kg of this infant formula milk powder, its raw material composition includes:

[0311] 850 kg of raw cow milk, 679.4 kg of the protein composition of Example 11, 25 kg of demineralized whey powder D90, 40 kg of corn oil, 50 kg of soybean oil, 140 kg of OPO structured lipid, 17 kg of fructooligosaccharide powder, 40 kg of galactooligosaccharide syrup, 21 kg of compound nutrients, 12 kg of DHA, 22 kg of ARA, 0.1 kg of Bifidobacterium (each kilogram of Bifidobacterium powder contains Bifidobacterium at 3×10 10 CFU or more);

[0312] Among them, the compound nutrients include about 3.5 kg of compound vitamin nutrient package, about 1 kg of choline chloride nutrient package, about 12 kg of mineral 2 nutrient package, about 1 kg of mineral 1 nutrient package, about 1.5 kg of magnesium chloride nutrient package and about 2 kg of potassium chloride nutrient package. The base material of each nutrient package is lactose.

[0313] The specific preparation process of the infant formula milk powder of this embodiment is as follows:

[0314] 1) Milk coarse filtration: After the milk is coarsely filtered and degassed in the balance cylinder, it is preheated by a plate heat exchanger and then separated from impurities by a separator.

[0315] 2) Milk homogenization and sterilization: After removing impurities, part of the milk enters the homogenizer for homogenization, and the other part is not homogenized. The homogenized milk and the unhomogenized milk are mixed and then enter the sterilization system for sterilization. After sterilization, they enter the mixing tank.

[0316] 3) Powder addition: Various powder raw materials are measured according to the formula and added into the powder mixing tank through the air delivery system, and then sucked into the mixing tank through the vacuum system.

[0317] 4) Melting and preparing oil: Put the oil specified in the formula into the carburizing room according to the formula requirements. The temperature in the carburizing room should be maintained at 50-90℃. After the oil is dissolved, pump it into the mixed oil storage tank (the temperature is 40-50℃, and the storage time is less than 12 hours to prevent fat oxidation). Then pump the mixed oil into the mixing tank through the oil pump according to the formula requirements.

[0318] 5) Dissolving and adding nutrients: Dissolve calcium powder, vitamins, minerals and other nutrient packages separately with purified water, and add them to the mixing tank in sequence; mix the various raw materials in the mixing tank to obtain a mixed liquid.

[0319] 6) Filtration: The mixed liquid is filtered through a filter to remove physical impurities that may be introduced into the raw materials.

[0320] 7) Homogenization: The mixed liquid is homogenized by a homogenizer, the first-level homogenization pressure is 105±5 bar, and the second-level homogenization pressure is 32±3 bar, the fat globules are mechanically treated and dispersed into uniform fat globules.

[0321] 8) Cooling and storage: The homogenized liquid enters the plate heat exchanger for cooling, and is cooled to below 20°C. It is temporarily stored in the pre-storage tank and enters the next process within 6 hours. The agitator is turned on according to the set requirements.

[0322] 9) Concentration and sterilization: Double-effect concentration is used during production, the sterilization temperature is ≥83°C, and the sterilization time is 25 seconds; the discharge concentration is 50% dry matter.

[0323] 10) Concentrated milk storage, preheating filtration, spray drying: The concentrated milk is temporarily stored in a concentrated milk balance tank; it is preheated to 60°C by a scraper preheater, and the preheated material is filtered through a filter with a pore size of 1mm, and then pumped into a drying tower for spray drying by a high-pressure pump. The fine powder is agglomerated at the top of the tower or in a fluidized bed as required; wherein, the inlet air temperature is 180°C, the exhaust air temperature is 86°C, the high-pressure pump pressure is 200bar, and the tower negative pressure is about -4mba.

[0324] 11) Fluidized bed drying and cooling: The powder coming out of the drying tower is dried twice in a fluidized bed (primary stage) and then cooled to 30°C in a fluidized bed (secondary stage); at the same time, the lecithin is mixed with the carrier and heated to 60°C. Under the action of compressed air, the lecithin is evenly dispersed on the surface of the milk powder, so that the milk powder particles agglomerate to increase their particle size and solubility.

[0325] 12) Packaging: According to the formula requirements, weigh the DHA, ARA or bifidobacteria and seal the bags for packaging.

[0326] 13) Dry mixing: Mix the weighed DHA, ARA or bifidobacteria with milk powder in a dry mixer.

[0327] 14) Powder screening: Use a vibrating screen to make the particle size of the milk powder uniform and discard the powder residue.

[0328] 15) Powder output: Collect the powder in a sterilized powder box and transport it from the powder output room to the powder loading room.

[0329] 16) Powdering: Pour the milk powder into the powder storage tank on the large and small packaging machines according to the packaging requirements.

[0330] 17) Packaging: 400g cans are packed with nitrogen by automatic packaging machine. The oxygen content is less than 1% when filled with nitrogen; 900g cans are packed with nitrogen by automatic packaging machine. The oxygen content is less than 5%.

[0331] 18) Packing: Put the packaged sachets into cartons and add the powder spoon, then seal with a carton sealing machine.

[0332] 19) Finished product inspection: Sampling inspection is carried out on the packaged products according to the inspection plan.

[0333] 20) Storage: Products that have passed the inspection shall be stored in warehouses at room temperature with humidity ≤ 65%.

[0334] The protein composition in the infant formula milk powder obtained in this embodiment has a whey protein content of 70% and a casein content of 30%, based on a total protein content of 100%;

[0335] The total protein content of the infant formula milk powder obtained in this example is 10.50 g / 100 g, the α-lactalbumin content is 2.218 g / 100 g, and the β-casein content is 3.156 g / 100 g.

[0336] Embodiment 14

[0337] This embodiment provides an infant formula milk powder, which contains a protein composition capable of improving the digestibility of protein in the infant formula milk powder. Based on the total weight of the infant formula milk powder being 1000 kg, the raw material composition thereof includes:

[0338] 1000 kg of raw milk, 674.1 kg of the protein composition of Example 4, 35 kg of sunflower oil, 50 kg of soybean oil, 100 kg of OPO structured fat, 13 kg of oligofructose powder, 25 kg of galacto-oligosaccharide syrup, 18.85 kg of compound nutrients, 12 kg of DHA, 22 kg of ARA, and 0.1 kg of bifidobacterium;

[0339] Among them, the compound nutrients include about 3.5 kg of compound vitamin nutrient package, about 1.5 kg of choline chloride nutrient package, about 10 kg of calcium powder nutrient package, about 1 kg of mineral nutrient package, about 0.85 kg of magnesium chloride nutrient package and about 2 kg of potassium chloride nutrient package. The base material of each nutrient package is lactose.

[0340] The preparation process of the infant formula milk powder of this embodiment is the same as that of Example 13.

[0341] The protein composition in the infant formula milk powder obtained in this embodiment has a whey protein content of 60%, a casein content of 40%, and a total protein content of 100%;

[0342] The total protein content of the infant formula milk powder obtained in this example is 16.53 g / 100 g, the α-lactalbumin content is 4.236 g / 100 g, and the β-casein content is 5.161 g / 100 g.

[0343] Embodiment 15

[0344] This embodiment provides an infant formula milk powder, which contains a protein composition capable of improving the digestibility of protein in the infant formula milk powder. Based on the total weight of the infant formula milk powder being 1000 kg, the raw material composition thereof includes:

[0345] 850 kg of raw milk, 675.8 kg of the protein composition of Example 3, 20 kg of corn oil, 40 kg of soybean oil, 85 kg of OPO structured fat, 17 kg of oligofructose powder, 40 kg of galacto-oligosaccharide syrup, 13 kg of compound nutrients, 8.6 kg of DHA, 15 kg of ARA, and 0.1 kg of bifidobacterium;

[0346] Among them, the compound nutrients include about 3.5 kg of compound vitamin nutrient package, about 0.75 kg of choline chloride nutrient package, about 5 kg of calcium powder nutrient package, about 1 kg of mineral nutrient package, about 0.75 kg of magnesium chloride nutrient package and about 2 kg of potassium chloride nutrient package. The base material of each nutrient package is lactose.

[0347] The preparation process of the infant formula milk powder of this embodiment is the same as that of Example 13.

[0348] The protein composition in the infant formula milk powder obtained in this embodiment has a whey protein content of 60%, a casein content of 40%, and a total protein content of 100%;

[0349] The total protein content of the infant formula milk powder obtained in this example is 15.92 g / 100 g, the α-lactalbumin content is 3.43 g / 100 g, and the β-casein content is 4.61 g / 100 g.

[0350] Example 16

[0351] This embodiment provides an infant formula milk powder, which contains a protein composition capable of improving the digestibility of protein in the infant formula milk powder. Based on the total weight of the infant formula milk powder being 1000 kg, the raw material composition thereof includes:

[0352] 1000 kg of raw milk, 671 kg of the protein composition of Example 7, 50 kg of corn oil, 65 kg of soybean oil, 35 kg of sunflower oil, 6 kg of oligofructose powder, 50 kg of galacto-oligosaccharide syrup, 17 kg of compound nutrients, 10 kg of DHA, 12 kg of ARA, and 0.1 kg of bifidobacterium;

[0353] Among them, the compound nutrients include about 3.5 kg of compound vitamin nutrient package, about 0.75 kg of choline chloride nutrient package, about 9 kg of calcium powder nutrient package, about 1 kg of mineral nutrient package, about 0.75 kg of magnesium chloride nutrient package and about 2 kg of potassium chloride nutrient package. The base material of each nutrient package is lactose.

[0354] The preparation process of the infant formula milk powder of this embodiment is the same as that of Example 13.

[0355] The protein composition in the infant formula milk powder obtained in this embodiment has a whey protein content of 35% and a casein content of 65%, based on a total protein content of 100%.

[0356] The total protein content of the infant formula milk powder obtained in this example is 14.58 g / 100 g, the α-lactalbumin content is 1.829 g / 100 g, and the β-casein content is 2.521 g / 100 g.

[0357] Embodiment 17

[0358] This embodiment provides an infant formula milk powder, which contains a protein composition capable of improving the digestibility of protein in the infant formula milk powder. Based on the total weight of the infant formula milk powder being 1000 kg, the raw material composition thereof includes:

[0359] 1000 kg of raw milk, 675 kg of the protein composition of Example 9, 40 kg of corn oil, 68 kg of soybean oil, 55 kg of sunflower oil, 12 kg of fructooligosaccharide powder, 20 kg of galacto-oligosaccharide syrup, 13 kg of compound nutrients, 10 kg of DHA, 12 kg of ARA, and 0.1 kg of bifidobacterium;

[0360] Among them, the compound nutrients include about 3.5 kg of compound vitamin nutrient package, about 0.75 kg of choline chloride nutrient package, about 5 kg of calcium powder nutrient package, about 1 kg of mineral nutrient package, about 0.75 kg of magnesium chloride nutrient package and about 2 kg of potassium chloride nutrient package. The base material of each nutrient package is lactose.

[0361] The preparation process of the infant formula milk powder of this embodiment is the same as that of Example 13.

[0362] The protein composition in the infant formula milk powder obtained in this embodiment has a whey protein content of 50%, a casein content of 50%, and a total protein content of 100%;

[0363] The total protein content of the infant formula milk powder obtained in this example is 14.16 g / 100 g, the α-lactalbumin content is 3.118 g / 100 g, and the β-casein content is 3.027 g / 100 g.

[0364] Embodiment 18

[0365] This embodiment provides an infant formula milk powder, which contains a protein composition capable of improving the digestibility of protein in the infant formula milk powder. Based on the total weight of the infant formula milk powder being 1000 kg, the raw material composition thereof includes:

[0366] 3000 kg of raw milk, 675.9 kg of the protein composition of Example 10, 80 kg of OPO structured fat, 25 kg of oligofructose powder, 30 kg of galacto-oligosaccharide syrup, 13 kg of compound nutrients, 8 kg of DHA, 2 kg of phospholipids, 10 kg of ARA, and 0.1 kg of bifidobacteria;

[0367] Among them, the compound nutrients include about 2.5 kg of compound vitamin nutrient package, about 1.1 kg of choline chloride nutrient package, about 8 kg of calcium powder nutrient package, and about 1 kg of mineral nutrient package. The base material of each nutrient package is lactose.

[0368] The preparation process of the infant formula milk powder of this embodiment is the same as that of Example 13.

[0369] The protein composition in the infant formula milk powder obtained in this embodiment has a whey protein content of 50%, a casein content of 50%, and a total protein content of 100%;

[0370] The total protein content of the infant formula milk powder obtained in this example is 21.52 g / 100 g, the α-lactalbumin content is 3.465 g / 100 g, and the β-casein content is 3.527 g / 100 g.

Claims

1. An infant formula milk powder, comprising a protein composition capable of improving the digestibility of protein in the infant formula milk powder, wherein the improving the digestibility of protein in the infant formula milk powder comprises increasing the proportion of released essential amino acids EAA in released free amino acids; The total protein content in this infant formula is 10.51-16.06 g / 100g; in, Taking the total protein content of the infant formula milk powder as 100%, the protein composition contains 28.9%-31.9% of α-lactalbumin and 26.0%-32.0% of β-casein; The content of α-lactalbumin in the infant formula milk powder is 2.208-5.117 g / 100 g, and the content of β-casein is 3.027-5.127 g / 100 g; Taking the total protein content of the infant formula milk powder as 100%, the content of whey protein in the protein composition is 35%-60%, and the content of casein in the protein composition is 40%-65%.

2. The infant formula milk powder according to claim 1, wherein Based on 1000 parts by weight of infant formula milk powder, the added amount of the protein composition is 540-690 parts by weight.

3. The infant formula milk powder according to claim 1 or 2, wherein: The protein composition is prepared from one or more raw materials selected from the group consisting of liquid milk, milk powder, whey protein powder and casein powder.

4. The infant formula milk powder according to claim 3, wherein: The milk powder includes whole milk powder and / or skimmed milk powder.

5. The infant formula milk powder according to claim 3, wherein: The liquid milk includes whole milk and / or skim milk.

6. The infant formula milk powder according to claim 3, wherein: The raw material also contains lactose.

7. The infant formula milk powder according to claim 1, wherein: The fat content of the infant formula is 15-29g / 100g; Linoleic acid content is 1800-5000mg / 100g; α-linolenic acid content is 200-500mg / 100g; Dietary fiber content is 0.95-6.3g / 100g; The carbohydrate content is 50-58g / 100g.

8. The infant formula milk powder according to claim 7, wherein: The dietary fiber includes galacto-oligosaccharide and fructo-oligosaccharide.

9. The infant formula milk powder according to claim 1, wherein: The protein source of the infant formula milk powder comprises one or a combination of two or more of milk, whole milk powder, skimmed milk powder, whey protein powder, desalted whey powder, and the protein composition.

10. The infant formula milk powder according to claim 9, wherein: Based on 1000 parts by weight of infant formula milk powder, the raw materials include: 800-3500 parts by weight of milk and 0-400 parts by weight of skimmed milk powder.

11. The infant formula milk powder according to claim 10, wherein: Part or all of the milk and skimmed milk powder are replaced with whole milk powder and / or skimmed milk with equivalent protein content.

12. The infant formula milk powder according to claim 1, wherein: The protein composition is composed of at least one of the following protein powders and / or milk powders: whey protein powder with an alpha-lactalbumin content of 25%-95%, whey protein powder with a beta-casein content of 35%-80%, whey protein powder with a protein content of 60%-95%, and milk powder with a protein content of 15%-50%.

13. The infant formula milk powder according to claim 12, wherein: The milk powder is skimmed milk powder.

14. The infant formula milk powder according to claim 12, wherein: The protein composition is composed of at least one of the following protein powders and / or milk powders: (1) Whey protein powder with an α-lactalbumin content of 25%-55%; (2) Whey protein powder with an α-lactalbumin content of 75%-95%; (3) Whey protein powder with a β-casein content of 35%-70%; (4) Whey protein powder with a β-casein content of 40%-80%; (5) Whey protein powder with a protein content of 60%-95%; (6) Milk powder with a protein content of 15%-50%.

15. The infant formula milk powder according to claim 9, wherein: Based on 1000 parts by weight of infant formula milk powder, the raw materials include: 0-170 parts by weight of whey protein powder, 25-400 parts by weight of desalted whey powder, 0-55 parts by weight of alpha-lactalbumin powder and 0-45 parts by weight of beta-casein powder.

16. The infant formula milk powder according to claim 1, wherein: The fat source of the infant formula comprises milk fat and / or anhydrous butter fat.

17. The infant formula milk powder according to claim 16, wherein: The fat source of the infant formula milk powder also includes vegetable oil and / or OPO structured fat.

18. The infant formula milk powder according to claim 17, wherein: The vegetable oil includes one or a combination of two or more of sunflower oil, corn oil, soybean oil, rapeseed oil, coconut oil, palm oil and walnut oil.

19. The infant formula milk powder according to claim 18, wherein: The vegetable oil includes one or a combination of two or more of sunflower oil, corn oil and soybean oil.

20. The infant formula milk powder according to claim 19, wherein: Based on 1000 parts by weight of infant formula milk powder, the raw materials include: 0-80 parts by weight of sunflower oil, 0-40 parts by weight of corn oil, 0-80 parts by weight of soybean oil, 0-140 parts by weight of OPO structured fat, and 0-4 parts by weight of anhydrous butter.

21. The infant formula milk powder according to claim 18 or 20, wherein: The contents of linoleic acid and α-linolenic acid in the sunflower seed oil are 7.6-8.9% and 0.25-0.38% respectively; The contents of linoleic acid and α-linolenic acid in the corn oil are 53.0-56.20% and 0.9-1.6%, respectively; The contents of linoleic acid and α-linolenic acid in the soybean oil are 50.0-53.5% and 7.6-9.6%, respectively; The contents of linoleic acid and α-linolenic acid in the OPO structural lipid are 5.9-6.3% and 0.4-0.62% respectively; The contents of linoleic acid and α-linolenic acid in the low-erucic acid rapeseed oil are 16-19% and 8.0-10.6%, respectively; The contents of linoleic acid and α-linolenic acid in the coconut oil are 1-3% and 0-1% respectively; The effective content of 1,3-dioleyl-2-palmityl triglyceride in the raw material of the OPO structured lipid is 40%-70%.

22. The infant formula milk powder according to claim 1, wherein: The raw materials of the infant formula milk powder also include lactose.

23. The infant formula milk powder according to claim 22, wherein: Based on 1000 parts by weight of infant formula milk powder, the raw materials include: 125-550 parts by weight of lactose.

24. The infant formula milk powder according to claim 1, wherein: The raw materials of the infant formula milk powder also include one or a combination of two or more of DHA, ARA, nucleotides and lactoferrin.

25. The infant formula milk powder according to claim 24, wherein: Based on 1000 parts by weight of infant formula milk powder, the raw materials include: 2-15 parts by weight of DHA, 3-22 parts by weight of ARA, 0-1.4 parts by weight of lactoferrin, and 0-0.7 parts by weight of nucleotides.

26. The infant formula milk powder according to claim 1, wherein: The raw materials of the infant formula milk powder also include compound nutrients including calcium powder, vitamins and minerals.

27. The infant formula milk powder according to claim 26, wherein: Based on 1000 parts by weight of infant formula milk powder, the raw materials include: 7-22 parts by weight of compound nutrients including calcium powder, vitamins and minerals.

28. The infant formula milk powder according to claim 26, wherein: The compound nutrients are added in the form of at least a compound vitamin package, calcium powder, and a mineral nutrient package; 1) the compound vitamin nutrient package, the content of each component in each gram of the compound vitamin nutrient package is as follows: Taurine: 140-340mg Vitamin A: 1700-5800 μg RE Vitamin D: 25-70 μg Vitamin B1: 3000-6800 μg Vitamin B2: 3500-6900 μg Vitamin B6: 2400-4000 μg Vitamin B12: 8-20 μg Vitamin K1: 200-700 μg Vitamin C: 155-700mg Vitamin E: 10-70 mg α-TE Nicotinamide: 10000-41550 μg Folic acid: 500-920 μg Biotin: 100-245 μg Pantothenic acid: 7100-25230 μg Inositol: 0-250mg L-Carnitine: 0-60mg 2) Mineral 2 Nutrition Pack, the content of each component in each gram of Mineral 2 Nutrition Pack is as follows: Calcium: 300-455mg Phosphorus: 75-150 mg 3) Mineral nutrition pack. The content of each component in each gram of mineral nutrition pack is as follows: Iron: 40-110 mg Zinc: 23-90 mg Copper: 2600-4180 μg Iodine: 500-995 μg Selenium: 0-200 μg Manganese: 0-579 μg 4) Compound magnesium chloride nutrient pack, each gram of compound magnesium chloride nutrient pack contains: Magnesium: 80-170mg 5) Compound potassium chloride nutrient pack. Each gram of compound potassium chloride nutrient pack contains: Potassium: 400-580 mg; 6) Choline chloride nutrient pack. Each gram of choline chloride nutrient pack contains: Choline: 300-950 mg.

29. The infant formula milk powder according to claim 28, wherein: The compound nutrients are added in the form of at least the following nutrient packages.

30. The infant formula milk powder according to claim 28, wherein: Based on 1000 parts by weight of infant formula milk powder, the added amount of the compound vitamin nutrient package is 2-4 parts by weight, the added amount of the mineral 2 nutrient package is 2-12 parts by weight, the added amount of the mineral 1 nutrient package is 0.5-3 parts by weight, the added amount of the compound magnesium chloride nutrient package is 0-2 parts by weight, the added amount of the compound potassium chloride nutrient package is 0-4.5 parts by weight, and the added amount of choline chloride nutrient is 0-2 parts by weight.

31. The infant formula milk powder according to claim 30, wherein: The base material of each nutrient pack is lactose or sodium L-ascorbate.

32. The infant formula milk powder according to claim 1, wherein: The raw materials of this infant formula also include probiotics.

33. The infant formula milk powder according to claim 32, wherein: The probiotics are bifidobacteria.

34. The infant formula milk powder according to claim 33, wherein: Based on 1000 parts by weight of infant formula milk powder, the raw materials include: 0.1-0.2 parts by weight of bifidobacterium.

35. The infant formula milk powder according to claim 34, wherein: Based on 1000 parts by weight of infant formula milk powder, the raw materials include: 0.18-0.2 parts by weight of bifidobacterium.

36. The infant formula milk powder according to claim 34, wherein: Each weight portion of Bifidobacterium powder contains 3×10 10 CFU or above.

37. The infant formula milk powder according to claim 1, wherein: Based on 1000 parts by weight of the infant formula milk powder, the raw materials of the infant formula milk powder include: 800-3500 parts by weight of raw milk; Lactose 125-330 parts by weight; 0-150 parts by weight of whole milk powder; Skimmed milk powder 0-400 parts by weight; Whey protein powder WPC80% 0-170 parts by weight; Whey protein powder WPC34% 0-150 parts by weight; Desalted whey powder 25-400 parts by weight; 0-80 parts by weight of sunflower oil; Corn oil 0-40 parts by weight; 20-80 parts by weight of soybean oil; OPO structural fat 0-140 parts by weight; 4-55 parts by weight of α-lactalbumin powder; β-casein powder 0-40 parts by weight; Soybean lecithin 0-5 parts by weight Anhydrous butter 0-2 parts by weight; 4-18 parts by weight of oligofructose powder; Galactooligosaccharide syrup 10-42 parts by weight; 7-22 parts by weight of a compound nutrient comprising calcium powder, vitamins and minerals; DHA 2-15 parts by weight; ARA 3-22 parts by weight; Nucleotides 0-0.7 parts by weight; Bifidobacterium 0.1-0.2 parts by weight.

38. The infant formula milk powder according to claim 1, wherein: This infant formula milk powder is suitable for infants and children aged 0-7 years old.

39. A method for preparing the infant formula milk powder according to any one of claims 1 to 38, comprising the following steps: The milk, powder raw materials and melted oil raw materials that have been coarsely filtered, homogenized and sterilized are mixed, and compound nutrients are added to obtain a mixed liquid; The mixed liquid is filtered, homogenized, cooled, concentrated and sterilized, spray-dried, and fluidized bed dried and cooled to obtain dry milk powder, which is mixed with DHA, ARA, lactoferrin, nucleotides, and bifidobacteria, and sieved to obtain the infant formula milk powder.

40. The preparation method according to claim 39, wherein The first-level pressure of the homogenization treatment of the mixed liquid is 105±5bar, and the second-level pressure is 32±3bar.

41. The preparation method according to claim 39, wherein The concentrated sterilization adopts double-effect concentration.

42. The preparation method according to claim 41, wherein The concentrated sterilization has a sterilization temperature of ≥83°C and a sterilization time of 25 seconds.

43. The preparation method according to claim 41, wherein The discharge concentration of the concentrated sterilization is 48%-52% dry matter.

44. The preparation method according to claim 39, wherein The inlet air temperature of the spray drying is 165-180°C, the exhaust air temperature is 75-90°C, the high-pressure pump pressure is 160-210 bar, and the tower negative pressure is -4 mbar to -2 mbar.

45. The preparation method according to claim 39, wherein Fluidized bed drying and cooling includes two drying and cooling steps. The temperature of the milk powder after the second drying and cooling step is 25-30°C. At the same time, the phospholipids are mixed with the carrier and heated to 60-65°C. Under the action of compressed air, the mixture is evenly dispersed on the surface of the milk powder.

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