A new Ganoderma lucidum strain ZL76 and its use

Abstract

The present invention provides a new Ganoderma lucidum strain ZL76 and its use, and relates to the field of traditional Chinese medicine. The Ganoderma lucidum strain ZL76 is a Ganoderma lucidum with a preservation number of CGMCC No. 23882 preserved in the General Microbiological Center of the China Microbiological Culture Collection Administration. The Ganoderma lucidum strain ZL76 of the present invention has the characteristics of stable genetic traits, high yield and excellent quality. At the same time, the Ganoderma lucidum strain has a significant inhibitory effect on the activity of melanoma cells and can be used to inhibit melanoma; in addition, the Ganoderma lucidum strain has a good inhibitory effect on tyrosinase activity and melanin production, and has a whitening effect. The Ganoderma lucidum strain ZL76 of the present invention can be used to prepare drugs that inhibit melanoma, inhibit tyrosinase activity and have whitening effects, can also be used to prepare cosmetics with whitening effects, and can also be used to prepare food or health food, and has good application prospects.

Description

Technical Field

[0001] The invention relates to the field of traditional Chinese medicine, and in particular to a new Ganoderma lucidum strain ZL76 and uses thereof. Background Art

[0002] Ganoderma lucidum (Ganodermas p.) belongs to the genus Ganoderma, Polyporales, Polyporaceae, Basidiomycetes, and is an important medicinal fungus that is widely used in traditional Chinese medicine. Its pharmacological effects have been discussed in many ways. The Shennong's Herbal Classic records that Ganoderma lucidum has the effects of strengthening the body, nourishing and strengthening the body, and prolonging life. Modern pharmacology and clinical trials have further proved that Ganoderma lucidum has anti-aging, anti-viral, anti-oxidant, anti-tumor, immunity-enhancing, and liver-protecting effects. Ganoderma lucidum and Ganoderma rubrum are the only two Ganoderma lucidum species included in the Pharmacopoeia of the People's Republic of China.

[0003] The Pharmacopoeia of the People's Republic of China (Part I, 2020 edition) stipulates that the moisture content of Ganoderma lucidum shall not exceed 17.0%, the total ash content shall not exceed 3.2%, the extract shall not be less than 3.0%, the Ganoderma lucidum polysaccharide shall not be less than 0.9%, and the triterpenoid and sterol content shall not be less than 0.5% in terms of oleanolic acid. However, the origin and variety of Ganoderma lucidum make the quality of Ganoderma lucidum in my country vary greatly, and the efficacy varies greatly. For example, although some Ganoderma lucidum has whitening effect, not all Ganoderma lucidum varieties have whitening effect.

[0004] Finding high-quality Ganoderma lucidum strains that can inhibit melanin and have whitening effects plays an important role in the development of the Ganoderma lucidum industry. Summary of the invention

[0005] The purpose of the present invention is to provide a new Ganoderma lucidum strain ZL76 and its application.

[0006] The Ganoderma lucidum strain ZL76 provided by the present invention is a Ganoderma lucidum deposited in the General Microbiological Center (CGMCC) of the China Microbiological Culture Collection Administration Committee with a deposit number of CGMCC No. 23882. The deposit address is No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, the deposit date is November 29, 2021, and the classification name is Ganoderma lucidum.

[0007] The present invention also provides a ganoderma lucidum extract, which is an ethanol extract obtained by extracting the aforementioned ganoderma lucidum strain with ethanol; or a water extract obtained by extracting the aforementioned ganoderma lucidum strain with water.

[0008] Furthermore, the preparation method of the alcohol extract comprises the following steps:

[0009] Take the dried Ganoderma lucidum mentioned above, grind it, add 40-50 times (g / mL) of ethanol to soak for 0.5-3h, then reflux extract for 1-5h, and collect the extract by suction filtration; add 40-50 times (g / mL) of ethanol to the residue and reflux extract for 1-5h, after suction filtration, evaporate the filtrate under reduced pressure, and then evaporate to dryness to obtain the alcohol extract.

[0010] Furthermore, the preparation method of the water extract comprises the following steps:

[0011] The filter residue after the alcohol extraction is dried in the air, and 40-50 times (g / mL) of water is added for reflux extraction twice, each time for 1-5 hours, the extracts are combined, filtered, evaporated under reduced pressure, and finally evaporated to dryness to obtain a water extract.

[0012] The present invention also provides the use of the aforementioned Ganoderma lucidum strain or the aforementioned Ganoderma lucidum extract in the preparation of a drug for preventing and / or treating melanoma;

[0013] Preferably, the drug is a drug that inhibits the activity of melanoma cells.

[0014] The present invention also provides the use of the aforementioned Ganoderma lucidum strain or the aforementioned Ganoderma lucidum extract in preparing a medicine with whitening effect and / or a cosmetic with whitening effect.

[0015] Furthermore, the drug is a drug that inhibits tyrosinase activity and melanin production.

[0016] The present invention also provides the use of the aforementioned Ganoderma lucidum strain or the aforementioned Ganoderma lucidum extract in the preparation of health food and / or food.

[0017] The present invention also provides a health food, cosmetic or pharmaceutical preparation, which is prepared by using the aforementioned Ganoderma lucidum strain or the aforementioned Ganoderma lucidum extract as an active ingredient and adding health food, cosmetic or pharmaceutically acceptable excipients or auxiliary ingredients.

[0018] The present invention also provides a food, which is prepared by adding the aforementioned ganoderma lucidum strain or the aforementioned ganoderma lucidum extract to auxiliary materials or auxiliary ingredients acceptable to food.

[0019] The present invention provides a new strain of Ganoderma lucidum ZL76, which has the characteristics of stable genetic traits, high yield and excellent quality. At the same time, the strain of Ganoderma lucidum has a significant inhibitory effect on the activity of melanoma cells and can be used to inhibit melanoma; in addition, the strain of Ganoderma lucidum has a good inhibitory effect on tyrosinase activity and melanin production, and has a whitening effect. The strain of Ganoderma lucidum ZL76 of the present invention can be used to prepare drugs for inhibiting melanoma, inhibiting tyrosinase activity and having whitening effects, can also be used to prepare cosmetics with whitening effects, and can also be used to prepare foods or health foods, and has good application prospects.

[0020] Obviously, according to the above contents of the present invention, in accordance with common technical knowledge and customary means in the art, without departing from the above basic technical ideas of the present invention, other various forms of modification, replacement or change may be made.

[0021] The above contents of the present invention are further described in detail below through specific implementation methods in the form of embodiments. However, this should not be understood as the scope of the above subject matter of the present invention being limited to the following examples. All technologies realized based on the above contents of the present invention belong to the scope of the present invention. DETAILED DESCRIPTION

[0022] Unless otherwise specified, the raw materials and equipment used in the specific embodiments of the present invention are all known products and are obtained by purchasing commercially available products.

[0023] Example 1: Ganoderma lucidum strain ZL76 of the present invention

[0024] The Ganoderma lucidum strain ZL76 of the present invention is deposited in the General Microbiology Center of the China Culture Collection Administration (CGMCC), the deposit address is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, the deposit date is November 29, 2021, the deposit number is CGMCC NO.23882, and the classification name is Ganoderma lucidum.

[0025] The main characteristics of this Ganoderma strain:

[0026] 1. Appearance

[0027] It has typical morphological characteristics of Ganoderma lucidum (Leyss.ex Fr.) Karst. The fruiting body is umbrella-shaped, with a kidney-shaped cap, 10-16cm in diameter and 1-2cm thick. The skin is hard, light yellow-brown, shiny, with annular ridges and radial wrinkles, thin and flat edges, often slightly rolled inward, and yellow-white on the ventral side.

[0028] 2. Production cycle: about 90 days.

[0029] 3. Yield: According to the conventional bag material cultivation technology, the yield is ≥59.60g / kg.bag material.

[0030] 4. Quality: After testing, the extract is 6.42%, polysaccharides are 0.93%, triterpenes and sterols are 0.89%. All tests should comply with the standards of the "Pharmacopoeia of the People's Republic of China" (2020 edition, Part One).

[0031] Example 2: Preparation of the alcohol extract of Ganoderma lucidum of the present invention

[0032] Take the dried Ganoderma lucidum fruiting body of Example 1, cut into pieces and crush. Weigh 5 g, add 250 ml of ethanol and soak for half an hour, reflux extraction for 1 hour, collect the extract, add 200 ml of ethanol and continue to reflux for 1 hour, filter with suction, evaporate the filtrate under reduced pressure and evaporate to dryness to obtain an alcohol extract.

[0033] Example 3: Preparation of the Ganoderma lucidum aqueous extract of the present invention

[0034] The residue after alcohol extraction and filtration in Example 2 was air-dried, and reflux-extracted twice with 200 ml of water, each time for 1 hour. The extracts were combined, filtered, evaporated under reduced pressure, and finally evaporated to dryness to obtain a water extract.

[0035] The beneficial effects of the present invention are demonstrated below through specific test examples.

[0036] Experimental Example 1: Study on the characteristics and quality of the Ganoderma lucidum strain ZL76 of the present invention

[0037] 1. Production cycle: about 90 days.

[0038] 2. Appearance: It has typical morphological characteristics of Ganoderma lucidum (Leyss.ex Fr.) Karst. The fruiting body is umbrella-shaped, with a kidney-shaped cap, 10-16cm in diameter and 1-2cm thick. The skin is hard, light yellow-brown, shiny, with annular ridges and radial wrinkles, thin and flat edges, often slightly rolled inward, and yellow-white on the ventral side.

[0039] 3. Yield: According to the conventional bag material cultivation technology, the yield is ≥59.60g / kg.bag material.

[0040] 4. Quality: After testing, the extract is 6.42%, polysaccharides are 0.93%, triterpenes and sterols are 0.89%. All tests should comply with the standards of the "Pharmacopoeia of the People's Republic of China" (2020 edition, Part One).

[0041] The experimental results show that the genetic characteristics of this Ganoderma lucidum strain are stable and consistent, meet the standards of the "Pharmacopoeia of the People's Republic of China", and are characterized by high yield and high quality.

[0042] Example 2: Study on the whitening effect of Ganoderma lucidum strains

[0043] 1. Experimental Materials

[0044] Take the Ganoderma lucidum strain of Example 1 of the present invention and the control Ganoderma lucidum strain, a total of 2 strains. The Ganoderma lucidum strain of the present invention is numbered ZL76, and the control Ganoderma lucidum strain is numbered ZL214. Use alcohol extraction and water extraction methods to obtain experimental samples, a total of 4 experimental samples (ZL76 alcohol extract, ZL76 water extract, ZL214 alcohol extract, ZL214 water extract).

[0045] The deposit information of the Ganoderma lucidum strain numbered ZL76 is: it is deposited in the General Microbiological Center of the China Microbiological Culture Collection Administration (CGMCC) with the deposit number CGMCC No.23882, and the deposit date is November 29, 2021. The deposit address is No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, and the classification name is Ganoderma lucidum.

[0046] The deposit information of the Ganoderma lucidum strain numbered ZL214 is: it is deposited in the General Microbiological Center of the China Microbiological Culture Collection Administration (CGMCC) with the deposit number CGMCC No.23881, and the deposit date is November 29, 2021. The deposit address is No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, and the classification name is Ganoderma lucidum.

[0047] The alcohol extraction method specifically includes the following steps: take dried Ganoderma lucidum fruiting bodies, cut into pieces and crush. Weigh 5g of each, add 250ml of ethanol to soak for half an hour, reflux extract for 1 hour, collect the extract, add 200ml of ethanol to continue reflux for 1 hour, filter with suction, evaporate the filtrate under reduced pressure and evaporate to dryness to obtain the alcohol extract.

[0048] The water extraction method specifically includes the following steps: drying the filter residue after alcohol extraction and filtration, refluxing and extracting twice with 200 ml of water, each time for 1 hour, combining the extracts, filtering and evaporating under reduced pressure, and finally evaporating to dryness to obtain a water extract.

[0049] For samples derived from alcohol extracts, add 1 ml of DMSO per 100 mg. Store at -20°C.

[0050] For each 10 mg of the aqueous extract, 1 ml of sterile water was added, the samples were sterilized by filtration, and stored at -20°C.

[0051] 2. Experimental methods

[0052] (1) Cell culture

[0053] B16 cells (mouse melanoma cells) were purchased from the Institute of Cell Biology, Chinese Academy of Sciences. The culture medium used was 90% DMEM high-glucose culture medium (containing 1× non-essential amino acids, 2mM glutamine, 1mM sodium pyruvate, 1.5g / L sodium bicarbonate) + 10% fetal bovine serum. Take the B16 cells in the logarithmic growth phase and aspirate the original culture medium, add 0.05% trypsin-0.53mM EDTA to digest, and wait for 2-5 minutes until the cells can be blown away by the culture medium. Add fresh culture medium and blow repeatedly to mix. Passage at a ratio of 1:4.

[0054] (2) Sample measurement

[0055] All the following operations were performed under sterile conditions and all instruments used were sterile.

[0056] ① Effect on B16 cell proliferation

[0057] Cell plating

[0058] Take B16 cells in the logarithmic growth phase, aspirate the original culture medium, add 0.05% trypsin-0.53mM EDTA to digest, and wait for 2-5 minutes until the cells can be blown off by the culture medium. Add fresh culture medium and blow repeatedly, mix well and transfer to the penicillin bottle. Take 20μl of cell suspension into EP tube, add 180μl trypan blue solution, mix well. Examine and count under a microscope.

[0059] Take an appropriate amount of cell suspension into a 50ml glass bottle, add fresh culture medium to make up the volume to 18ml, add 180μl of the diluted cell suspension to each well of a 96-well cell culture plate, and make the number of cells in each well 6×10 3 The cell plate was placed in a CO2 incubator and incubated overnight.

[0060] Add sample

[0061] Take 2 μl of the obtained Ganoderma lucidum sample, thaw at room temperature, shake to dissolve, mix well, and obtain the sample mother solution. According to the predicted experimental results, determine the starting concentration range of each sample, and set up a solvent control according to the sample addition concentration. Dilute the sample mother solution 100 times to obtain application solution 1, and dilute application solution 1 4 times to obtain application solution 2.

[0062] Set 5 sample concentration gradients for each sample, and repeat each gradient 3 times. Add application solution 1 or application solution 2 to a 96-well cell culture plate, and supplement with PBS to 20 μl, so that the final sample concentrations are 12.5 μg / ml, 25 μg / ml, 50 μg / ml, 75 μg / ml, and 100 μg / ml, respectively. Add 20 μl PBS to the blank control well. Mix well. Incubate the cell plate in a CO2 incubator for 48 hours.

[0063] MTT assay

[0064] 4 hours before the end of cell culture, remove the cell culture plate, carefully and slowly remove the culture medium, add 100 μl of PBS, add 10 μl of MTT solution (5 mg / ml), and mix well.

[0065] After the cell plate was incubated in a CO2 incubator for 4-6 hours, 100 μl of SDS solution was added to each well. The cell plate was incubated in a CO2 incubator overnight. The OD value was measured at 570 nm using an ELISA reader.

[0066] ②Effects on tyrosinase activity of B16 cells

[0067] Cell plating

[0068] Take B16 cells in the logarithmic growth phase, aspirate the original culture medium, add 0.05% trypsin-0.53mM EDTA to digest, and wait for 2-5 minutes until the cells can be blown off by the culture medium. Add fresh culture medium and blow repeatedly, mix well and transfer to the penicillin bottle. Take 20μl of cell suspension into EP tube, add 180μl trypan blue solution, mix well. Examine and count under a microscope.

[0069] Take an appropriate amount of cell suspension into a 50ml glass bottle, add fresh culture medium to make up the volume to 18ml, add 180μl of the diluted cell suspension to each well of a 96-well cell culture plate, and make the number of cells in each well 6×10 3 The cell plate was placed in a CO2 incubator and incubated overnight.

[0070] Induction

[0071] Theophylline was added to each well of cells at a final concentration of 1 mM.

[0072] Add sample

[0073] Take 2 μl of the obtained Ganoderma lucidum sample, thaw at room temperature, shake to dissolve, mix well, and obtain the sample mother solution. According to the predicted experimental results, determine the starting concentration range of each sample, and set up a solvent control according to the sample addition concentration. Dilute the sample mother solution 100 times to obtain application solution 1, and dilute application solution 1 4 times to obtain application solution 2.

[0074] Set 5 sample concentration gradients for each sample, and repeat each gradient 3 times. Add application solution 1 or application solution 2 to a 96-well cell culture plate, and supplement with PBS to 20 μl, so that the final sample concentrations are 12.5 μg / ml, 25 μg / ml, 50 μg / ml, 75 μg / ml, and 100 μg / ml, respectively. Add 20 μl PBS to the blank control well. Mix well. Incubate the cell plate in a CO2 incubator for 48 hours.

[0075] Tyrosinase assay

[0076] After the cell culture is completed, remove the cell culture plate. Carefully and slowly remove the culture medium, add 100μl of 1% Triton X-100 solution, and place at -80℃ for 60min. Remove the cell plate, return to room temperature, take 10μl to determine the protein content using the BCA method. Take another 80μl, add 50ul of 0.1% L-Dopa solution, incubate at 37℃ for 2h, and use an enzyme reader to measure the OD value at 490nm.

[0077] ③Effects on melanin production in B16 cells

[0078] Cell plating

[0079] Take B16 cells in the logarithmic growth phase, aspirate the original culture medium, add 0.05% trypsin-0.53mM EDTA to digest, and wait for 2-5 minutes until the cells can be blown off by the culture medium. Add fresh culture medium and blow repeatedly, mix well and transfer to the penicillin bottle. Take 20μl of cell suspension into EP tube, add 180μl trypan blue solution, mix well. Examine and count under a microscope.

[0080] Take an appropriate amount of cell suspension into a 50ml glass bottle, add fresh culture medium to make up the volume to 18ml, add 180μl of the diluted cell suspension to each well of a 96-well cell culture plate, and make the number of cells in each well 6×10 3 The cell plate was placed in a CO2 incubator and incubated overnight.

[0081] Add sample

[0082] Take an appropriate amount of Ganoderma lucidum sample, thaw it at room temperature, shake it to dissolve, mix it, and obtain the sample mother solution. According to the predicted experimental results, determine the starting concentration range of each sample, and set up a solvent control according to the sample addition concentration.

[0083] Theophylline was added to each well of cells at a final concentration of 1 mM.

[0084] Set up 3 sample concentration gradients for each sample. Add samples to a 96-well cell culture plate and add PBS to 20 μl, so that the final sample concentrations are 12.5 μg / ml, 25 μg / ml, and 50 μg / ml, respectively. Add 20 μl PBS to the blank control well. Mix well. Incubate the cell plate in a CO2 incubator for 72 hours.

[0085] Melanogenesis test

[0086] After the cell culture is completed, remove the cell culture plate, carefully and slowly remove the culture medium, wash twice with PBS, add 50μl of 1mol / L NaOH, and place at 37℃ for 60min. Measure the OD value at 460nm using an enzyme marker.

[0087] 3. Experimental results

[0088] (1) Effects on B16 cell proliferation

[0089] The experimental results are shown in Table 1 and Table 2: The inhibitory effects of the two alcohol extracts of Ganoderma lucidum on B16 cell proliferation are stronger than those of the water extracts, and there is a relatively obvious dose-effect relationship, indicating that the inhibitory effect of the alcohol extract of Ganoderma lucidum on B16 cells is better than that of the water extract. At the same time, the inhibitory effects of the water extract and alcohol extract of Ganoderma lucidum numbered ZL76 on B16 cell proliferation are stronger than those of Ganoderma lucidum numbered ZL214, indicating that the inhibitory effect of Ganoderma lucidum ZL76 on B16 cells is better than that of Ganoderma lucidum ZL214, and further indicating that the inhibitory effect of Ganoderma lucidum ZL76 on melanoma is better than that of Ganoderma lucidum ZL214. The water extract of Ganoderma lucidum ZL214 has basically no effect on inhibiting B16 cells.

[0090] Table 1. Inhibitory effect of Ganoderma lucidum water extract on B16 cell proliferation (%)

[0091]

[0092]

[0093] Table 2. Inhibitory effect of Ganoderma lucidum alcohol extract on B16 cell proliferation (%)

[0094]

[0095] (2) Inhibitory effect on tyrosinase activity of B16 cells

[0096] The experimental results are shown in Tables 3 and 4: The water extract and alcohol extract of Ganoderma lucidum numbered ZL76 have a good inhibitory effect on the tyrosinase activity of B16 cells, and there is an obvious dose-effect relationship; the water extract and alcohol extract of Ganoderma lucidum numbered ZL214 do not have a significant inhibitory effect on the tyrosinase activity of B16 cells. Tyrosinase is an oxidase and a rate-limiting enzyme that regulates the production of melanin. This enzyme participates in the synthesis of melanin. Inhibiting tyrosinase can inhibit melanin synthesis and thus achieve a whitening effect. The experimental results show that Ganoderma lucidum numbered ZL76 has a significant inhibitory effect on tyrosinase, which further shows that it can inhibit the production of melanin and has a whitening effect; while Ganoderma lucidum numbered ZL214 does not have the above effect.

[0097] Table 3. Inhibitory effect of Ganoderma lucidum water extract on tyrosinase activity of B16 cells (%)

[0098]

[0099] Table 4. Inhibitory effect of Ganoderma lucidum alcohol extract on tyrosinase activity of B16 cells (%)

[0100]

[0101] (3) Inhibitory effect on melanin production in B16 cells

[0102] The experimental results are shown in Table 5 and Table 6: The water extract and alcohol extract of Ganoderma lucidum numbered ZL76 both have an inhibitory effect on the production of melanin in B16 cells, and there is an obvious dose-effect relationship; the water extract and alcohol extract of Ganoderma lucidum numbered ZL214 have a very poor inhibitory effect on the production of melanin in B16 cells, and basically have no inhibitory effect on the production of melanin in B16 cells. Consistent with the above results, it shows that Ganoderma lucidum numbered ZL76 has a whitening effect, while Ganoderma lucidum numbered ZL214 does not have a whitening effect.

[0103] Table 5. Inhibitory effect of Ganoderma lucidum water extract samples on melanin production in B16 cells (%)

[0104]

[0105]

[0106] Table 6. Inhibitory effect of Ganoderma lucidum alcohol extract samples on melanin production in B16 cells (%)

[0107]

[0108] The above test results indicate that: compared with Ganoderma lucidum ZL214, Ganoderma lucidum ZL76 of the present invention has a significant inhibitory effect on the activity of melanoma cells and can be used to inhibit melanoma; meanwhile, compared with Ganoderma lucidum ZL214 which cannot inhibit tyrosinase activity and melanin production and has no whitening effect, Ganoderma lucidum ZL76 of the present invention has a good inhibitory effect on both tyrosinase activity and melanin production, indicating that it has a good whitening effect.

[0109] In summary, the present invention provides a new strain of Ganoderma lucidum ZL76, which has the characteristics of stable genetic traits, high yield and excellent quality. At the same time, the Ganoderma lucidum strain has a significant inhibitory effect on the activity of melanoma cells and can be used to inhibit melanoma; in addition, the Ganoderma lucidum strain has a good inhibitory effect on tyrosinase activity and melanin production, and has a whitening effect. The Ganoderma lucidum strain ZL76 of the present invention can be used to prepare drugs that inhibit melanoma, inhibit tyrosinase activity and have whitening effects, can also be used to prepare cosmetics with whitening effects, and can also be used to prepare foods or health foods, and has good application prospects.

Claims

1. A Ganoderma lucidum strain, characterized in that: It is the Ganoderma lucidum preserved in the General Microbiological Center of China Microorganism Culture Collection Administration with the preservation number of CGMCC No.23882.

2. A Ganoderma lucidum extract, characterized in that: The alcohol extract is obtained by extracting the ganoderma lucidum strain described in claim 1 with ethanol.

3. The Ganoderma lucidum extract according to claim 2, characterized in that: The preparation method of the alcohol extract comprises the following steps: Take the dried Ganoderma lucidum according to claim 1, calculate by mL / g, grind it, add 40-50 times the amount of ethanol, soak it for 0.5-3h, then reflux extract it for 1-5h, and collect the extract by suction filtration; add 40-50 times the amount of ethanol, calculate by mL / g, reflux extract it for 1-5h, filter it, evaporate the filtrate under reduced pressure, and then evaporate it to dryness to obtain an alcohol extract.

4. Use of the Ganoderma lucidum strain according to claim 1 or the Ganoderma lucidum extract according to any one of claims 2 or 3 in the preparation of a medicament for preventing and / or treating melanoma.

5. The use according to claim 4, characterized in that: The drug is a drug that inhibits the activity of melanoma cells.

6. Use of the Ganoderma lucidum strain according to claim 1 or the Ganoderma lucidum extract according to any one of claims 2 or 3 in the preparation of a medicine with whitening effect and / or a cosmetic with whitening effect.

7. The use according to claim 6, characterized in that: The drug is a drug that inhibits tyrosinase activity and inhibits melanin production.

8. Use of the Ganoderma lucidum strain according to claim 1 or the Ganoderma lucidum extract according to claim 2 or 3 in preparing food.

9. Use of the Ganoderma lucidum strain according to claim 1 or the Ganoderma lucidum extract according to claim 2 or 3 in preparing health food.

10. A health food, characterized in that: The health food is prepared by taking the ganoderma strain described in claim 1 or the ganoderma extract described in claim 2 or 3 as active ingredients and adding auxiliary materials acceptable to health foods.

11. A cosmetic, characterized in that: The cosmetic is prepared by using the ganoderma lucidum strain described in claim 1 or the ganoderma lucidum extract described in claim 2 or 3 as active ingredients and adding cosmetically acceptable auxiliary materials.

12. A pharmaceutical preparation, characterized in that: The invention is a pharmaceutical preparation prepared by taking the ganoderma strain described in claim 1 or the ganoderma extract described in claim 2 or 3 as active ingredients and adding pharmaceutically acceptable auxiliary materials.

13. A food, characterized in that: The invention relates to a food prepared by adding the ganoderma lucidum strain described in claim 1 or the ganoderma lucidum extract described in claim 2 or 3 to auxiliary materials acceptable to food.

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