A sunflower seed meal extract, its preparation method and application

Abstract

The invention discloses a sunflower seed meal extract and a preparation method and application thereof. The sunflower seed meal extract contains 6 phenolic acid compounds and 2 oligosaccharides, wherein the total content of the 6 phenolic acid compounds is more than 17wt%, and the total content of the 2 oligosaccharides is more than 33wt%. Since the composition ratio of the 6 phenolic acid compounds and the 2 oligosaccharides is reasonable, the sunflower seed meal extract does not need to be added with a stabilizer or embedded in nanoliposomes to ensure the stability of the sunflower seed meal extract. The cytotoxicity of the sunflower seed meal extract is very low, and the 6 phenolic acid compounds and the 2 oligosaccharides have a synergistic effect of resisting ultraviolet radiation damage. The sunflower seed meal extract of the invention is a highly efficient and low-toxic natural ultraviolet absorber, has good resistance to ultraviolet radiation damage and certain antioxidant effects, and can be applied to sunscreen and antioxidant cosmetics and external medicines.

Description

Technical Field

[0001] The invention relates to the technical field of sunflower seed meal extracts, and in particular to a sunflower seed meal extract and a preparation method and application thereof. Background Art

[0002] Sunflower (Helianthus annuus L.) is a plant of the genus Helianthus in the Asteraceae family. It is an important oil crop in my country, with a wide planting area and production all over the country. Sunflower seed meal is a byproduct of oil extraction from sunflower seeds. The annual output of sunflower seed meal in my country exceeds 400,000 tons (Zhang Weinong, Liu Dachuan. Comprehensive development of sunflower seed resources [J]. Journal of Wuhan Polytechnic Institute, 2002 (1): 29-30 + 35.). Literature reports show that sunflower seeds contain phenolic acid compounds [Shuangshuang Guo, Yan Ge, Kriskamol Na Jom. A review of phytochemistry, metadata changes, and medicinal uses of the common sunfower seed and sprouts (Helianthus annuus L.) [J]. Chemistry Central Journal, 2017, (11): 95. DOI 10.1186 / s13065-017-0328-7]. Literature reports show that phenolic acid compounds can be extracted from sunflower seed meal. However, due to the instability of chlorogenic acid itself, high temperature, strong light and long-term heating are not allowed during extraction. In addition, considering the instability of chlorogenic acid itself, stabilizers are often used in many preparation methods for extracting chlorogenic acid. In addition, in order to ensure the stability of chlorogenic acid, stabilizers are usually added or nanoliposomes are used for encapsulation. These will undoubtedly increase the production cost of chlorogenic acid, and there will be many unstable factors.

[0003] For example, the patent document (CN201810898013.3) discloses a method for extracting chlorogenic acid from fresh leaves of Eucommia ulmoides using biotechnology. In the preparation method, epoxy resin and chitosan are used as raw materials, and a purification resin is prepared by cross-linking polymerization. A layer of chitosan adsorption film is formed inside the resin. Through the double-layer adsorption of the resin and the adsorption film, the impurities in the chlorogenic acid can be completely adsorbed in the resin without destroying the structure and physiological activity of the chlorogenic acid. The stabilizer synthesized from polyglutamic acid and flavanols as raw materials can enhance the resistance of chlorogenic acid to external high temperature and high oxygen, and protect the chlorogenic acid from inactivation. The preparation method adds a stabilizer synthesized from polyglutamic acid and flavanols as raw materials, which brings many uncertainties, because the various effects of the stabilizer on the human body in this application are still unclear and difficult to control.

[0004] Patent document (CN201610541620.5) A method for embedding tobacco chlorogenic acid nanoliposomes, weighing chlorogenic acid, egg yolk lecithin and cholesterol according to the formula; dissolving with ethanol; injecting the ethanol solution into the phosphate buffer; removing the ethanol by rotary evaporation; short-time ultrasound to obtain chlorogenic acid liposomes. The embedding rate of chlorogenic acid reached 88.02%, the average particle size was 77.16nm, and the prepared chlorogenic acid liposome system was stable. Through the liposome embedding method, the chance of chlorogenic acid contacting with the external environment can be reduced or avoided, thereby protecting the chlorogenic acid. This method will obviously increase the production cost of chlorogenic acid.

[0005] The present invention identifies a sunflower seed meal extract, which contains 6 phenolic acid compounds and 2 oligosaccharides, and the sum of the contents of the phenolic acid compounds and oligosaccharides in the extract is greater than 50%. A safe, simple and efficient preparation method is established, and the sunflower seed meal extract prepared by the method has very low toxicity to skin cells, has high stability without adding a stabilizer during preparation, and has good resistance to ultraviolet radiation damage and certain antioxidant effects, and can be applied to sunscreen and antioxidant cosmetics and external medicines. Summary of the invention

[0006] The purpose of the present invention is to: in response to the above-mentioned problems, the present invention provides a sunflower seed meal extract with low production cost, very low toxicity to skin cells, high stability, good resistance to ultraviolet radiation damage and certain antioxidant effects, as well as a preparation method and application thereof.

[0007] The present invention is achieved through the following technical solutions:

[0008] The present invention provides: a sunflower seed meal extract, the sunflower seed meal extract comprises 6 phenolic acid compounds and 2 oligosaccharides; wherein the 6 phenolic acid compounds are chlorogenic acid, neochlorogenic acid, cryptochlorogenic acid, isochlorogenic acid A, isochlorogenic acid B, and isochlorogenic acid C; the 2 oligosaccharides are sucrose and raffinose;

[0009] In the sunflower seed meal extract, the total content of the six phenolic acid compounds is above 17wt%, and the total content of the two oligosaccharides is above 33wt%.

[0010] Furthermore, a sunflower seed meal extract is provided, wherein the contents of the components in the sunflower seed meal extract are as follows: the content of chlorogenic acid is above 8.80wt%, the content of neochlorogenic acid is above 3.10wt%, the content of cryptochlorogenic acid is above 4.40wt%, the content of isochlorogenic acid A is above 0.15wt%, the content of isochlorogenic acid B is above 0.20wt%, the content of isochlorogenic acid C is above 0.28wt%, the content of sucrose is above 22.10wt%, and the content of raffinose is above 11.50wt%; the ability of the sunflower seed meal extract to resist ultraviolet radiation damage is the result of the synergistic effect of the six phenolic acid compounds and the two oligosaccharides, and the ability of the sunflower seed meal extract to resist ultraviolet radiation damage is equivalent to that of high concentration chlorogenic acid, and is better than the ability of the chemically synthesized ultraviolet absorber benzophenone-3 to resist ultraviolet radiation damage.

[0011] The present invention also provides a method for preparing a sunflower seed meal extract, comprising the following steps:

[0012] (1) taking sunflower seed meal and crushing it into 200-300 meshes to obtain sunflower seed meal powder;

[0013] (2) shaking the sunflower seed meal powder at a frequency of 35 to 45 times / second and a temperature of 5 to 10° C. for 30 to 40 minutes to obtain a sunflower seed meal powder that has been shaken once; shaking the sunflower seed meal powder that has been shaken once for 25 to 30 minutes at a frequency of 50 to 55 times / second and a temperature of 5 to 10° C. to obtain a sunflower seed meal powder that has been shaken twice; shaking the sunflower seed meal powder that has been shaken twice for 20 to 25 minutes at a frequency of 25 to 30 times / second and a temperature of 5 to 10° C. to obtain a sunflower seed meal powder that has been shaken three times;

[0014] (3) extracting six phenolic acid compounds and two oligosaccharides from the sunflower seed meal powder shaken three times with ethanol aqueous solution, filtering and obtaining an extract;

[0015] (4) Concentrating and drying the extract to obtain the sunflower seed meal extract.

[0016] Furthermore, the step (1) also includes an operation to promote the stability of the six phenolic acid compounds in the sunflower seed meal, and the operation method is as follows: 1) one month before the sunflower seeds are harvested, 300-400 kg / mu of biogas slurry is applied every 3-5 days, and the sunflower plants are supplemented with light every night, and the supplementary light process parameters are: intermittent supplementary light, turn on the light for supplementary light for 1 hour, turn off the light for 30 minutes, turn on the light for supplementary light for 1 hour, and then turn off the light for 30 minutes, and repeat this until the supplementary light time reaches 4-5 hours; the number of supplementary light lamps per acre of sunflower is 200-250, the supplementary light lamp is 20 cm away from the sunflower plant, and the power of the supplementary light lamp is 25W; 2) harvest the sunflowers and put the sunflowers into trays for airing and drying to obtain sunflower seeds; 3) take the sunflower seed oil to obtain the sunflower seed meal.

[0017] Furthermore, the step (1) also includes an operation to promote the stability of the six phenolic acid compounds in the sunflower seed meal, and the operation method is as follows: 1) one month before the sunflower seeds are harvested, 350-400 kg / mu of biogas slurry is applied every 4-5 days, and the sunflower plants are supplemented with light at night every day, and the supplementary lighting process parameters are: intermittent supplementary lighting, each time the light is turned on for supplementary lighting for 1 hour, the light is turned off for 30 minutes, the light is turned on for supplementary lighting for 1 hour, and the light is turned off for 30 minutes, and this is repeated until the supplementary lighting time reaches 4-5 hours; the number of supplementary lighting lamps per acre of sunflower is 230-250, the supplementary lighting lamp is 20 cm away from the sunflower plant, and the power of the supplementary lighting lamp is 25W; 2) the sunflowers are harvested and the sunflowers are placed on trays for airing and drying to obtain sunflower seeds; 3) sunflower seed oil is taken to obtain sunflower seed meal.

[0018] Furthermore, 15-20 g / L of potassium humate is added to the biogas slurry in step (1).

[0019] Furthermore, in the step (2), the sunflower seed meal powder is shaken at a frequency of 40 to 45 times per second and a temperature of 8 to 10° C. for 35 to 40 minutes to obtain a sunflower seed meal powder that has been shaken once; the sunflower seed meal powder that has been shaken once is shaken at a frequency of 52 to 55 times per second and a temperature of 8 to 10° C. for 27 to 30 minutes to obtain a sunflower seed meal powder that has been shaken twice; the sunflower seed meal powder that has been shaken twice is shaken at a frequency of 28 to 30 times per second and a temperature of 8 to 10° C. for 22 to 25 minutes to obtain a sunflower seed meal powder that has been shaken three times.

[0020] Furthermore, the volume fraction of ethanol in the ethanol aqueous solution in step (3) is between 20% and 95%; and the method for extracting the sunflower seed meal powder after three shakes is reflux extraction, ultrasonic extraction or supercritical fluid extraction.

[0021] The invention also provides an application of the sunflower seed meal extract, which is applied to sunscreen cosmetics or sunscreen external medicines.

[0022] The invention also provides an application of the sunflower seed meal extract, which is applied to antioxidant cosmetics or antioxidant external medicines.

[0023] The invention discloses a sunflower seed meal extract and a preparation method and application thereof. The sunflower seed meal extract contains 6 phenolic acid compounds and 2 oligosaccharides, wherein the total content of the 6 phenolic acid compounds is more than 17wt%, the total content of the 2 oligosaccharides is more than 33wt%, and the sum of the contents of the 6 phenolic acid compounds and the 2 oligosaccharides in the extract is greater than 50wt%. Since the composition ratio of the 6 phenolic acid compounds and the 2 oligosaccharides is reasonable, the sunflower seed meal extract does not need to be added with a stabilizer or embedded in nanoliposomes to ensure the stability of the sunflower seed meal extract. Moreover, the 6 phenolic acid compounds are not affected by the non-utilization of the 2 oligosaccharides. In the presence of the 2 oligosaccharides, the sunflower seed meal extract still has very low toxicity to skin cells, has good resistance to ultraviolet radiation damage and certain anti-oxidation effects, and can be applied to sunscreen and anti-oxidation cosmetics and external medicines.

[0024] The invention discloses a sunflower seed meal extract and a preparation method and application thereof. The extract is subjected to a protective effect test on damage to human epidermal keratinocytes (HaCaT) caused by UVA and UVB co-radiation and damage to human skin fibroblasts (HSF) caused by UVA radiation. The test groups include a model group, a benzophenone-3 group (positive control group), a chlorogenic acid group (200 μg / mL), a sucrose group, and high, medium and low dose groups of sunflower seed meal extract. The content of 6 phenolic acids in the sunflower seed meal extract is about 17%. When the sunflower seed meal extract acts on cells at a concentration of 400, 200 and 100 μg / mL, the concentration of total phenolic acid is about 68, 34 and 17 μg / mL, which is much lower than 100 μg / mL. The results show that the three dose groups of sunflower seed meal extract have a preventive effect on ultraviolet radiation damage to HaCaT and HSF, and the survival rates are significantly different from those of the model group (P<0.01). Among them, the survival rate of the high-dose group was significantly different from that of the positive group (P < 0.01), and there was no significant difference compared with the chlorogenic acid group. It can be seen that the ability of the sunflower seed meal extract to resist ultraviolet radiation damage is the result of the synergistic effect of 6 phenolic acid compounds and 2 oligosaccharides. The ability of the sunflower seed meal extract described in this application to resist ultraviolet radiation damage is equivalent to that of high-concentration chlorogenic acid, and is far superior to the existing chemically synthesized ultraviolet absorber benzophenone-3.

[0025] The present invention discloses a sunflower seed meal extract and a preparation method and application thereof. Due to the instability of chlorogenic acid itself, high temperature, strong light and long-term heating are not allowed during extraction. In order to ensure the yield of chlorogenic acid itself and avoid oxidation of chlorogenic acid, when preparing the sunflower seed meal extract, the sunflower seed meal is first crushed and then subjected to segmented oscillation treatment, which can effectively destroy the cell wall of the sunflower seed meal, help dissolve 6 phenolic acid compounds and 2 oligosaccharides, and improve the extraction rate of phenolic acid, so that the total content of the 6 phenolic acid compounds in the extracted sunflower seed meal extract is above 17wt%, and the total content of the 2 oligosaccharides is above 33wt%. The sum of the contents of six phenolic acid compounds and two oligosaccharides in the extract is greater than 50wt%; due to the reasonable composition ratio of the six phenolic acid compounds and the two oligosaccharides, the stability of the sunflower seed meal extract is guaranteed, the cytotoxicity of the sunflower seed meal extract is very low, and the six phenolic acid compounds and the two oligosaccharides have a synergistic effect of resisting ultraviolet radiation damage; the sunflower seed meal extract of the invention is a highly efficient and low-toxic natural ultraviolet absorber, has good resistance to ultraviolet radiation damage and certain antioxidant effects, can be applied to sunscreen and antioxidant cosmetics and external medicines; and has the advantages of a simple preparation method and low production cost.

[0026] The invention discloses a sunflower seed meal extract and a preparation method and application thereof, which also include an operation of promoting the stability of six phenolic acid compounds in the sunflower seed meal. Biogas slurry and intermittent supplementary light are applied before the sunflower seeds are harvested. Microorganisms secrete a variety of active substances when decomposing and fermenting biogas slurry gas-producing raw materials. Under the joint action of light and the various active substances and trace elements in the biogas slurry, the synthesis and accumulation of the six phenolic acid compounds in the sunflower seeds can be promoted, and the stability of the generated six phenolic acid compounds can be effectively improved. 15-20 g / L of potassium humate is also added to the biogas slurry, which can assist in promoting the synthesis and accumulation of raffinose in the sunflower seeds, and further ensure the stability of the sunflower seed meal extract. DETAILED DESCRIPTION

[0027] Any feature disclosed in this specification (including any additional claims and abstract), unless otherwise stated, may be replaced by other equivalent or alternative features having similar purposes. That is, unless otherwise stated, each feature is only an example of a series of equivalent or similar features.

[0028] The present invention discloses a sunflower seed meal extract, which comprises 6 phenolic acid compounds and 2 oligosaccharides; wherein the 6 phenolic acid compounds are chlorogenic acid, neochlorogenic acid, cryptochlorogenic acid, isochlorogenic acid A, isochlorogenic acid B and isochlorogenic acid C; and the 2 oligosaccharides are sucrose and raffinose;

[0029] In the sunflower seed meal extract, the total content of the six phenolic acid compounds is above 17wt%, and the total content of the two oligosaccharides is above 33wt%;

[0030] More specifically, the content of chlorogenic acid is above 8.80wt%, the content of neochlorogenic acid is above 3.10wt%, the content of cryptochlorogenic acid is above 4.40wt%, the content of isochlorogenic acid A is above 0.15wt%, the content of isochlorogenic acid B is above 0.20wt%, the content of isochlorogenic acid C is above 0.28wt%, the content of sucrose is above 22.10wt%, and the content of raffinose is above 11.50wt%.

[0031] Example 1: Preparation of the sunflower seed meal extract

[0032] A method for preparing a sunflower seed meal extract comprises the following steps:

[0033] (1) First, an operation is performed to promote the stability of six phenolic acid compounds in sunflower seed meal, and the operation method is as follows: 1) one month before the sunflower seeds are harvested, 300 kg / mu of biogas slurry is applied every three days, and the sunflower plants are supplemented with light every night. The supplementary lighting process parameters are: intermittent supplementary lighting, each time the light is turned on for supplementary lighting for 1 hour, the light is turned off for 30 minutes, the light is turned on for supplementary lighting for 1 hour, and the light is turned off for 30 minutes, and this is repeated until the supplementary lighting time reaches 4 hours; the number of supplementary lighting lamps per mu of sunflower is 200, the supplementary lighting lamps are 20 cm away from the sunflower plants, and the power of the supplementary lighting lamps is 25 W; 2) the sunflowers are harvested and the sunflowers are placed on trays for airing and drying to obtain sunflower seeds; 3) the sunflower seed oil is extracted to obtain sunflower seed meal; 15 g / L of potassium humate is also added to the biogas slurry;

[0034] Then, the sunflower seed meal is taken and crushed into 200 meshes to obtain sunflower seed meal powder;

[0035] (2) shaking the sunflower seed meal powder at a frequency of 35 times / second and a temperature of 5°C for 30 minutes to obtain a sunflower seed meal powder that has been shaken once; shaking the sunflower seed meal powder that has been shaken once for 25 minutes at a frequency of 50 times / second and a temperature of 5°C to obtain a sunflower seed meal powder that has been shaken twice; shaking the sunflower seed meal powder that has been shaken twice for 20 minutes at a frequency of 25 times / second and a temperature of 5°C to obtain a sunflower seed meal powder that has been shaken three times;

[0036] (3) extracting six phenolic acid compounds and two oligosaccharides from the sunflower seed meal powder shaken three times with an ethanol aqueous solution having an ethanol volume fraction of 75%, filtering, and obtaining an extract; the method for extracting the sunflower seed meal powder shaken three times is reflux extraction; the total weight of the ethanol aqueous solution used for extraction is 15 times the weight of the sunflower seed meal powder shaken three times, and the extraction is divided into two times, in the first extraction, the total weight of the ethanol aqueous solution used is 10 times the weight of the sunflower seed meal powder shaken three times, and in the second extraction, the total weight of the ethanol aqueous solution used is 5 times the weight of the sunflower seed meal powder shaken three times;

[0037] (4) Concentrating and drying the extract, and recovering ethanol under reduced pressure to obtain the sunflower seed meal extract; the yield of the sunflower seed meal extract is 18.3%.

[0038] Example 2: Preparation of the sunflower seed meal extract

[0039] A method for preparing a sunflower seed meal extract comprises the following steps:

[0040] (1) First, an operation is performed to promote the stability of six phenolic acid compounds in sunflower seed meal, and the operation method is as follows: 1) one month before the sunflower seeds are harvested, 400 kg / mu of biogas slurry is applied every 5 days, and the sunflower plants are supplemented with light every night. The supplementary lighting process parameters are: intermittent supplementary lighting, each time the light is turned on for supplementary lighting for 1 hour, the light is turned off for 30 minutes, the light is turned on for supplementary lighting for 1 hour, and the light is turned off for 30 minutes, and this is repeated until the supplementary lighting time reaches 5 hours; the number of supplementary lighting lamps per acre of sunflower is 250, the supplementary lighting lamps are 20 cm away from the sunflower plants, and the power of the supplementary lighting lamps is 25 W; 2) the sunflowers are harvested and the sunflowers are placed on trays for airing and drying to obtain sunflower seeds; 3) the sunflower seed oil is extracted to obtain sunflower seed meal; 20 g / L of potassium humate is also added to the biogas slurry;

[0041] Then, the sunflower seed meal is taken and crushed into 300 meshes to obtain sunflower seed meal powder;

[0042] (2) shaking the sunflower seed meal powder at a frequency of 45 times / second and a temperature of 10°C for 40 minutes to obtain a sunflower seed meal powder that has been shaken once; shaking the sunflower seed meal powder that has been shaken once for 30 minutes at a frequency of 55 times / second and a temperature of 10°C to obtain a sunflower seed meal powder that has been shaken twice; shaking the sunflower seed meal powder that has been shaken twice for 25 minutes at a frequency of 30 times / second and a temperature of 10°C to obtain a sunflower seed meal powder that has been shaken three times;

[0043] (3) extracting six phenolic acid compounds and two oligosaccharides from the sunflower seed meal powder shaken three times with an ethanol aqueous solution having an ethanol volume fraction of 65%, filtering, and obtaining an extract; the method for extracting the sunflower seed meal powder shaken three times is supercritical fluid extraction; the total weight of the ethanol aqueous solution used for extraction is 15 times the weight of the sunflower seed meal powder shaken three times, and the extraction is divided into two times. In the first extraction, the total weight of the ethanol aqueous solution used for extraction is 10 times the weight of the sunflower seed meal powder shaken three times, and in the second extraction, the total weight of the ethanol aqueous solution used for extraction is 5 times the weight of the sunflower seed meal powder shaken three times;

[0044] (4) Concentrating and drying the extract, and recovering ethanol under reduced pressure to obtain the sunflower seed meal extract; the yield of the sunflower seed meal extract is 18.2%.

[0045] Example 3: Preparation of the sunflower seed meal extract

[0046] A method for preparing a sunflower seed meal extract comprises the following steps:

[0047] (1) First, an operation is performed to promote the stability of six phenolic acid compounds in sunflower seed meal, and the operation method is as follows: 1) one month before the sunflower seeds are harvested, 350 kg / mu of biogas slurry is applied every 4 days, and the sunflower plants are supplemented with light every night. The supplementary lighting process parameters are: intermittent supplementary lighting, each time the light is turned on for supplementary lighting for 1 hour, the light is turned off for 30 minutes, the light is turned on for supplementary lighting for 1 hour, and the light is turned off for 30 minutes, and this is repeated until the supplementary lighting time reaches 4 hours; the number of supplementary lighting lamps per acre of sunflower is 230, the supplementary lighting lamps are 20 cm away from the sunflower plants, and the power of the supplementary lighting lamps is 25 W; 2) the sunflowers are harvested and the sunflowers are placed on trays for airing and drying to obtain sunflower seeds; 3) the sunflower seed oil is extracted to obtain sunflower seed meal; 17 g / L of potassium humate is also added to the biogas slurry;

[0048] Then, the sunflower seed meal is taken and crushed to 250 meshes to obtain sunflower seed meal powder;

[0049] (2) shaking the sunflower seed meal powder at a frequency of 40 times / second and a temperature of 8°C for 35 minutes to obtain a sunflower seed meal powder that has been shaken once; shaking the sunflower seed meal powder that has been shaken once for 27 minutes at a frequency of 52 times / second and a temperature of 8°C to obtain a sunflower seed meal powder that has been shaken twice; shaking the sunflower seed meal powder that has been shaken twice for 22 minutes at a frequency of 28 times / second and a temperature of 8°C to obtain a sunflower seed meal powder that has been shaken three times;

[0050] (3) extracting six phenolic acid compounds and two oligosaccharides from the sunflower seed meal powder shaken three times with an ethanol aqueous solution having an ethanol volume fraction of 70%, filtering, and obtaining an extract; the method for extracting the sunflower seed meal powder shaken three times is ultrasonic extraction; the total weight of the ethanol aqueous solution used for extraction is 15 times the weight of the sunflower seed meal powder shaken three times, and the extraction is divided into two times, in the first extraction, the total weight of the ethanol aqueous solution used is 10 times the weight of the sunflower seed meal powder shaken three times, and in the second extraction, the total weight of the ethanol aqueous solution used is 5 times the weight of the sunflower seed meal powder shaken three times;

[0051] (4) Concentrating and drying the extract, and recovering ethanol under reduced pressure to obtain the sunflower seed meal extract; the yield of the sunflower seed meal extract is 18.7%.

[0052] Example 4: Preparation of the sunflower seed meal extract

[0053] A method for preparing a sunflower seed meal extract comprises the following steps:

[0054] (1) First, an operation is performed to promote the stability of six phenolic acid compounds in sunflower seed meal, and the operation method is as follows: 1) one month before the sunflower seeds are harvested, 350 kg / mu of biogas slurry is applied every 4 days, and the sunflower plants are supplemented with light every night. The supplementary lighting process parameters are: intermittent supplementary lighting, turn on the light for supplementary lighting for 1 hour, turn off the light for 30 minutes, turn on the light for supplementary lighting for 1 hour, and then turn off the light for 30 minutes, and repeat this process until the supplementary lighting time reaches 4 hours; the number of supplementary lighting lamps per acre of sunflower is 230, the supplementary lighting lamps are 20 cm away from the sunflower plants, and the power of the supplementary lighting lamps is 25 W; 2) the sunflowers are harvested and the sunflowers are placed on trays for airing and drying to obtain sunflower seeds; 3) the sunflower seed oil is extracted to obtain sunflower seed meal;

[0055] Then, the sunflower seed meal is taken and crushed to 250 meshes to obtain sunflower seed meal powder;

[0056] (2) shaking the sunflower seed meal powder at a frequency of 40 times / second and a temperature of 8°C for 35 minutes to obtain a sunflower seed meal powder that has been shaken once; shaking the sunflower seed meal powder that has been shaken once for 27 minutes at a frequency of 52 times / second and a temperature of 8°C to obtain a sunflower seed meal powder that has been shaken twice; shaking the sunflower seed meal powder that has been shaken twice for 22 minutes at a frequency of 28 times / second and a temperature of 8°C to obtain a sunflower seed meal powder that has been shaken three times;

[0057] (3) extracting six phenolic acid compounds and two oligosaccharides from the sunflower seed meal powder shaken three times with an ethanol aqueous solution having an ethanol volume fraction of 70%, filtering, and obtaining an extract; the method for extracting the sunflower seed meal powder shaken three times is ultrasonic extraction; the total weight of the ethanol aqueous solution used for extraction is 15 times the weight of the sunflower seed meal powder shaken three times, and the extraction is divided into two times, in the first extraction, the total weight of the ethanol aqueous solution used is 10 times the weight of the sunflower seed meal powder shaken three times, and in the second extraction, the total weight of the ethanol aqueous solution used is 5 times the weight of the sunflower seed meal powder shaken three times;

[0058] (4) Concentrating and drying the extract, and recovering ethanol under reduced pressure to obtain the sunflower seed meal extract; the yield of the sunflower seed meal extract is 18.1%.

[0059] Example 5: Preparation of the sunflower seed meal extract

[0060] A method for preparing a sunflower seed meal extract comprises the following steps:

[0061] (1) firstly, an operation is performed to promote the stability of six phenolic acid compounds in sunflower seed meal, wherein the operation method is as follows: 1) one month before the sunflower seeds are harvested, 350 kg / mu of biogas slurry is applied every 4 days; 2) the sunflowers are harvested and the sunflowers are placed in trays for airing and sun drying to obtain sunflower seeds; 3) sunflower seed oil is extracted to obtain sunflower seed meal; 17 g / L of potassium humate is also added to the biogas slurry;

[0062] Then, the sunflower seed meal is taken and crushed to 250 meshes to obtain sunflower seed meal powder;

[0063] (2) shaking the sunflower seed meal powder at a frequency of 40 times / second and a temperature of 8°C for 35 minutes to obtain a sunflower seed meal powder that has been shaken once; shaking the sunflower seed meal powder that has been shaken once for 27 minutes at a frequency of 52 times / second and a temperature of 8°C to obtain a sunflower seed meal powder that has been shaken twice; shaking the sunflower seed meal powder that has been shaken twice for 22 minutes at a frequency of 28 times / second and a temperature of 8°C to obtain a sunflower seed meal powder that has been shaken three times;

[0064] (3) extracting six phenolic acid compounds and two oligosaccharides from the sunflower seed meal powder shaken three times with an ethanol aqueous solution having an ethanol volume fraction of 70%, filtering, and obtaining an extract; the method for extracting the sunflower seed meal powder shaken three times is ultrasonic extraction; the total weight of the ethanol aqueous solution used for extraction is 15 times the weight of the sunflower seed meal powder shaken three times, and the extraction is divided into two times, in the first extraction, the total weight of the ethanol aqueous solution used is 10 times the weight of the sunflower seed meal powder shaken three times, and in the second extraction, the total weight of the ethanol aqueous solution used is 5 times the weight of the sunflower seed meal powder shaken three times;

[0065] (4) Concentrating and drying the extract, and recovering ethanol under reduced pressure to obtain the sunflower seed meal extract; the yield of the sunflower seed meal extract is 18.0%.

[0066] Example 6: Preparation of the sunflower seed meal extract

[0067] A method for preparing a sunflower seed meal extract comprises the following steps:

[0068] (1) First, an operation is performed to promote the stability of six phenolic acid compounds in sunflower seed meal, and the operation method is as follows: 1) One month before the sunflower seeds are harvested, the sunflower plants are supplemented with light every night, and the supplementary light process parameters are: intermittent supplementary light, each time the light is turned on for supplementary light for 1 hour, the light is turned off for 30 minutes, the light is turned on for supplementary light for 1 hour, and the light is turned off for 30 minutes, and this is repeated until the supplementary light time reaches 4 hours; the number of supplementary light lamps per acre of sunflower is 230, the supplementary light lamp is 20 cm away from the sunflower plant, and the power of the supplementary light lamp is 25 W; 2) The sunflowers are harvested and the sunflowers are placed on trays for drying to obtain sunflower seeds; 3) Sunflower seed oil is taken, that is, sunflower seed meal is obtained;

[0069] Then, the sunflower seed meal is taken and crushed to 250 meshes to obtain sunflower seed meal powder;

[0070] (2) shaking the sunflower seed meal powder at a frequency of 40 times / second and a temperature of 8°C for 35 minutes to obtain a sunflower seed meal powder that has been shaken once; shaking the sunflower seed meal powder that has been shaken once for 27 minutes at a frequency of 52 times / second and a temperature of 8°C to obtain a sunflower seed meal powder that has been shaken twice; shaking the sunflower seed meal powder that has been shaken twice for 22 minutes at a frequency of 28 times / second and a temperature of 8°C to obtain a sunflower seed meal powder that has been shaken three times;

[0071] (3) extracting six phenolic acid compounds and two oligosaccharides from the sunflower seed meal powder shaken three times with an ethanol aqueous solution having an ethanol volume fraction of 70%, filtering, and obtaining an extract; the method for extracting the sunflower seed meal powder shaken three times is ultrasonic extraction; the total weight of the ethanol aqueous solution used for extraction is 15 times the weight of the sunflower seed meal powder shaken three times, and the extraction is divided into two times, in the first extraction, the total weight of the ethanol aqueous solution used is 10 times the weight of the sunflower seed meal powder shaken three times, and in the second extraction, the total weight of the ethanol aqueous solution used is 5 times the weight of the sunflower seed meal powder shaken three times;

[0072] (4) Concentrating and drying the extract, and recovering ethanol under reduced pressure to obtain the sunflower seed meal extract; the yield of the sunflower seed meal extract is 18.1%.

[0073] Example 7

[0074] A method for preparing a sunflower seed meal extract comprises the following steps:

[0075] (1) taking sunflower seed meal and grinding it into 250 meshes to obtain sunflower seed meal powder;

[0076] (2) shaking the sunflower seed meal powder at a frequency of 40 times / second and a temperature of 8°C for 35 minutes to obtain a sunflower seed meal powder that has been shaken once; shaking the sunflower seed meal powder that has been shaken once for 27 minutes at a frequency of 52 times / second and a temperature of 8°C to obtain a sunflower seed meal powder that has been shaken twice; shaking the sunflower seed meal powder that has been shaken twice for 22 minutes at a frequency of 28 times / second and a temperature of 8°C to obtain a sunflower seed meal powder that has been shaken three times;

[0077] (3) extracting six phenolic acid compounds and two oligosaccharides from the sunflower seed meal powder shaken three times with an ethanol aqueous solution having an ethanol volume fraction of 70%, filtering, and obtaining an extract; the method for extracting the sunflower seed meal powder shaken three times is ultrasonic extraction; the total weight of the ethanol aqueous solution used for extraction is 15 times the weight of the sunflower seed meal powder shaken three times, and the extraction is divided into two times, in the first extraction, the total weight of the ethanol aqueous solution used is 10 times the weight of the sunflower seed meal powder shaken three times, and in the second extraction, the total weight of the ethanol aqueous solution used is 5 times the weight of the sunflower seed meal powder shaken three times;

[0078] (4) Concentrating and drying the extract, and recovering ethanol under reduced pressure to obtain the sunflower seed meal extract; the yield of the sunflower seed meal extract is 17.8%.

[0079] Comparative Example 1

[0080] A method for preparing a sunflower seed meal extract comprises the following steps:

[0081] (1) taking sunflower seed meal and grinding it into 250 meshes to obtain sunflower seed meal powder;

[0082] (2) extracting six phenolic acid compounds and two oligosaccharides from the sunflower seed meal powder with an ethanol aqueous solution having an ethanol volume fraction of 70%, filtering, and obtaining an extract; the method for extracting the sunflower seed meal powder is ultrasonic extraction; the total weight of the ethanol aqueous solution used for extraction is 15 times the weight of the sunflower seed meal powder, and the extraction is performed twice, in which the total weight of the ethanol aqueous solution used for the first extraction is 10 times the weight of the sunflower seed meal powder, and in the second extraction, the total weight of the ethanol aqueous solution used is 5 times the weight of the sunflower seed meal powder;

[0083] (3) Concentrating and drying the extract, and recovering ethanol under reduced pressure to obtain the sunflower seed meal extract; the yield of the sunflower seed meal extract is 17.4%.

[0084] The determination method of each component in the sunflower seed meal extract of the present invention is:

[0085] (1) Determination of phenolic acid compounds

[0086] Chromatographic conditions: ultra-high performance liquid chromatography; chromatographic column: C 18 Column (2.1×100mm, 1.8μm); mobile phase: methanol (A)-0.1% formic acid (B); gradient elution program: 0-3min: 15%-25% A, 3-5min: 25%-30% A, 5-8min: 30%-33% A, 8-10min: 33%-40% A; stop time 40min; post-run 10min; detection wavelength: 328nm; column temperature 20℃; injection volume 2μL; flow rate: 0.12mL / min.

[0087] Preparation of mixed reference solution: Use methanol to dissolve and prepare mixed reference solutions with concentrations of 0.0990 mg / mL, 0.0268 mg / mL, 0.0369 mg / mL, 0.0021 mg / mL, 0.0026 mg / mL, 0.0032 mg / mL for chlorogenic acid, 0.0268 mg / mL, 0.0369 mg / mL, 0.0021 mg / mL, 0.0026 mg / mL, and 0.0032 mg / mL, respectively.

[0088] Preparation of test solution: Accurately weigh about 1 g of sunflower seed meal extract, sonicate with 10.0 mL of methanol for 30 min, cool, reweight, and pass the supernatant through a microporous filter membrane for later use.

[0089] Determination of samples: Accurately pipette 2 μL of the mixed reference solution and the test solution, inject the sample according to the above chromatographic conditions to determine the peak area, and calculate the content of each phenolic acid in the test sample. The results are shown in Table 1.

[0090] (2) Determination of oligosaccharide content

[0091] Chromatographic conditions: high performance liquid chromatograph (evaporative light scattering detector); chromatographic column: amino column; mobile phase: 70% acetonitrile-30% pure water; running time: 20 min; flow rate: 0.7 mL / min; ELSD: 60°C, 1.5 mL / min, gain: 4.

[0092] Preparation of mixed reference solution: Dissolve sucrose and raffinose reference substances in methanol to prepare a mixed reference solution with concentrations of 2.520 mg / mL and 2.519 mg / mL, respectively.

[0093] Preparation of test solution: Accurately weigh about 0.1 g of sunflower seed meal extract, sonicate with 30.0 mL of pure water for 40 min, cool, reweight, and pass the supernatant through a microporous filter membrane for later use.

[0094] Determination of samples: accurately pipette 5 μL of the mixed reference solution and the test solution, inject the sample according to the above chromatographic conditions to determine the peak area, and calculate the content of sucrose and raffinose in the test sample;

[0095] Using the above detection method, the results of the determination of the contents of phenolic acid and oligosaccharides in the sunflower seed meal extracts in Examples 1-7 are shown in Table 1 below:

[0096] Table 1 Contents of various phenolic acids and oligosaccharides in sunflower seed meal extract

[0097]

[0098] The results of determination of the contents of phenolic acids and oligosaccharides in the sunflower seed meal extract in the comparative example were as follows: the content of chlorogenic acid was 8.75wt%, the content of neochlorogenic acid was 3.07wt%, the content of cryptochlorogenic acid was 4.35wt%, the content of isochlorogenic acid A was 0.13wt%, the content of isochlorogenic acid B was 0.18wt%, the content of isochlorogenic acid C was 0.24wt%, the content of sucrose was 24.37wt%, and the content of raffinose was 7.65wt%;

[0099] The stability of the loaded sunflower seed meal extracts prepared in the above Examples 1-7 and Comparative Example 1 is shown in Table 2 below;

[0100] Table 2 Stability study results of sunflower seed meal extract

[0101] Thermal stability (65℃) Alkali stability Storage stability (three months) Example 1 73.4％ 72.5％ 93.2 Example 2 73.5％ 72.3％ 93.5 Example 3 73.8％ 72.8％ 93.8 Example 4 71.2％ 70.4％ 90.9 Example 5 70.3％ 70.6％ 90.8 Example 6 70.2％ 70.4％ 90.5 Example 7 69.7％ 68.5％ 87.6 Comparative Example 1 62.8％ 63.7％ 76.9

[0102] Experimental study on the sunflower seed meal extract prepared by the method of Example 1 to prevent ultraviolet radiation damage

[0103] (1) Cytotoxicity of sunflower seed meal extract on human epidermal keratinocytes (HaCaT) and human skin fibroblasts (HSF)

[0104] Take HaCaT or HSF cells with a confluency of more than 80%, digest them, and adjust the cell concentration to 3-4×10 cells / mL using culture medium (DMEM high-glucose medium containing 10% FBS and 1% double antibody). 5 or 1~2×10 5 100 μL of cell suspension was added to each well of a 96-well plate and placed in a 5% CO 2, cultured in a 37°C incubator for 24 hours. Benzophenone-3 (CAS: 131-57-7), chlorogenic acid, sucrose and sunflower seed meal extracts were prepared into 50 mg / mL stock solutions with DMSO, appropriately diluted with culture medium and added to each well. 100 μL of sample solution was added to each well. The final concentrations of each sample after addition were 400, 300, 200, 160, 80, 40, 20, and 10 μg / mL, respectively. Three replicate wells were set for each concentration. After the addition, the culture was continued for 24 hours, the culture medium was aspirated, 100 μL of 10% CCK-8 solution was added to each well, and after culturing for 2 hours, the OD value was measured at 450 nm, the inhibition rate was calculated, and the half inhibition rate IC was calculated using statistical software. 50 The inhibition rate calculation formula is as follows, and the results are shown in Tables 3 and 4.

[0105] Inhibition rate % = [1-(OD 给药 -OD 空白 ) / (OD 细胞对照 -OD 空白 )]×100%

[0106] Table 3 HaCaT cytotoxicity test results

[0107] Sample name <![CDATA[IC 50 (μg / mL)]]> Benzophenone-3 305.94±1.79 Chlorogenic acid ＞400 sucrose ＞400 Sunflower Seed Meal Extract ＞400

[0108] Table 4 HSF cytotoxicity test results

[0109] Sample name <![CDATA[IC 50 (μg / mL)]]> Benzophenone-3 99.08±1.99 Chlorogenic acid ＞400 sucrose ＞400 Sunflower Seed Meal Extract ＞400

[0110] The results showed that the sunflower seed meal extract had an IC 50 All of them were greater than 400 μg / mL, which was much smaller than the IC of the positive control benzophenone-3. 50 (305.94μg / mL and 99.08μg / mL), proving that sunflower seed meal extract had little toxicity to both skin cells.

[0111] (2) Protective effect of sunflower seed meal extract against HaCaT radiation damage caused by UVB and UVA

[0112] Take HaCaT cells with a confluency of more than 80%, digest them, and adjust the cell concentration to 3-4×10 with culture medium (DMEM high-glucose medium containing 10% FBS and 1% double antibody). 5 / mL, inoculated in a 96-well plate, and 100μL of cell suspension was added to each well. A model group that received radiation, a sunflower seed meal extract administration group, a chlorogenic acid group, a sucrose group, a dibenzophenone-3 positive control group, and a cell control group that did not receive radiation were set up, and each group had 3 replicate wells. After the cells were plated and cultured for 24 hours, the supernatant was aspirated, and 100μL of each sample solution diluted with PBS to an appropriate concentration was added to the administration group, and 100μL of PBS was added to both the model group and the control group. The final concentrations of the administration groups were 400, 200 and 100μg / mL, respectively. The lids of the cells in each group that received radiation were opened and irradiated under a certain energy of UVB and UVA, respectively. The radiation energy UVB was 5mJ / cm 2 , UVA is 150mJ / cm 2 , the radiation energy calculation formula is:

[0113] Radiation dose (mJ / cm 2 ) = radiation intensity (mw / cm 2 )×irradiation time (sec)

[0114] After the irradiation was completed, the supernatant was aspirated and 100 μL of culture medium was added to each well for further 24 h. The cell control group was subjected to the same operation steps except that it was not irradiated. After 24 h, the CCK-8 method was used for color development and the survival rate was calculated. The survival rate calculation formula is as follows. The results are shown in Table 5.

[0115] Survival rate % = (OD 辐射组 -OD 空白 ) / (OD 细胞对照 -OD 空白 )×100%

[0116] Table 5 Protective effect of sunflower seed meal extract on HaCaT damage caused by UVB and UVA radiation

[0117]

[0118]

[0119] Note: * indicates significant difference compared with the model group, P < 0.05; ** indicates extremely significant difference compared with the model group, P < 0.01. # Compared with the positive group, there is a significant difference, P < 0.05. ## It indicates that there is a very significant difference compared with the positive group, P < 0.01.

[0120] The results showed that the three dose groups of sunflower seed meal extract had a preventive effect on HaCaT radiation damage caused by UVB and UVA, and the survival rate was significantly different from that of the model group (P < 0.01). The survival rates of the high-dose group and the medium-dose group were significantly better than the existing chemically synthesized ultraviolet absorber benzophenone-3 (positive group) (P < 0.01). The representative phenolic acid compound contained in the sunflower seed meal extract, chlorogenic acid, and the representative oligosaccharide compound contained in it, sucrose, both have a certain preventive effect on HaCaT from ultraviolet radiation.

[0121] The sunflower seed meal extract prepared in Example 1 of the present invention was used to test the protective effect of HSF radiation damage caused by UVA. The grouping and operation methods of the test were the same as those of HaCaT. The radiation energy of UVA was 10 J / cm 2 , the results are shown in Table 6.

[0122] Table 6 Protective effect of sunflower seed meal extract on HSF damage caused by UVA radiation

[0123] Group Concentration (μg / mL) Survival rate (%) Model Group - 43.04±2.13 Benzophenone-3 group (positive control group) 200 64.21±1.78** Chlorogenic acid group 200 <![CDATA[84.43±2.36** ## ]]> Sucrose group 200 <![CDATA[53.35±2.34** # ]]> High-dose sunflower seed meal extract 400 <![CDATA[85.56±2.45** ## ]]> Sunflower seed meal extract medium dose group 200 68.13±1.54** Sunflower seed meal extract low dose group 100 61.24±2.37**

[0124] Note: * indicates significant difference compared with the model group, P < 0.05; ** indicates extremely significant difference compared with the model group, P < 0.01. # Compared with the positive group, there is a significant difference, P < 0.05. ## It indicates that there is a very significant difference compared with the positive group, P < 0.01.

[0125] The results showed that the three dose groups of sunflower seed meal extract had a preventive effect on HSF radiation damage caused by UVA, and the survival rate was significantly different from that of the model group (P < 0.01). The survival rate of the high-dose group was significantly different from that of the positive group (P < 0.01). The representative compound of phenolic acid contained in the sunflower seed meal extract, chlorogenic acid, and the representative compound of oligosaccharides contained in it, sucrose, both have a certain preventive effect on HSF from ultraviolet radiation.

[0126] Summary of the above experiments on preventing UV radiation damage:

[0127] From the experimental results, it can be seen that the toxicity of sunflower seed meal extract to skin cells is very low, far lower than the existing commonly used UV absorber benzophenone-3. Whether it is HaCaT cell damage caused by UVB and UVA, or HSF cell damage caused by UVA, sunflower seed meal extract has a good preventive effect, and its preventive effect is significantly better than benzophenone-3. In addition, although the survival rate of the sucrose group is lower, it is still significantly different from that of the model group (P < 0.01), indicating that oligosaccharides also have a certain effect on resisting ultraviolet radiation damage. From the content determination results, it can be seen that the content of 6 phenolic acids in sunflower seed meal extract is about 17%. When the sunflower seed meal extract acts on cells at a concentration of 400, 200, and 100 μg / mL, the concentration of total phenolic acid is about 68, 34, and 17 μg / mL, which is far lower than 100 μg / mL. It can be seen that the ability of sunflower seed meal extract to resist ultraviolet radiation damage is the result of the synergistic effect of 6 phenolic acid compounds and 2 oligosaccharides.

[0128] Experimental study on the antioxidant effect of the sunflower seed meal extract prepared in Example 1 of the present invention

[0129] (1) Determination of DPPH free radical scavenging ability

[0130] The DPPH free radical scavenging ability was determined according to the reference method (Zhao L, Kim JC, Hur J S. 7-Hydroxy-2-octenoic acid-ethylester mixture as an UV protectant secondary metabolite of an endolichenic fungus isolated from Menegazziaterebrata[J]. Archives of Microbiology, 2022, 204(7): 395.). The results are shown in Table 7.

[0131] (2)ABTS + Free radical scavenging assay

[0132] Reference method (Li Qi, Wu Chaoquan, Pei Hehuan. Effect of salt roasting on the spectrum-effect relationship of Guangxi Emblica emblica scavenging two free radicals [J]. Chinese Journal of Hospital Pharmacy, 2023, 43(2): 168-174.) ABTS + The free radical scavenging ability of free radicals was determined. The results are shown in Table 7.

[0133] (3) Hydroxyl radical scavenging assay

[0134] The hydroxyl radical scavenging ability was determined according to the reference method (Huang Shangfeng, Lu Feiyan, Huang Yuanhe, Pan Qiaodan, Zhang Xiangui, Huang Suoyi. Research on the antioxidant activity of Panax notoginseng polysaccharide [J]. Trace Elements and Health Research, 2018, 35(1): 30-32.) The results are shown in Table 7.

[0135] Table 7 Antioxidant test results

[0136]

[0137]

[0138] Note: * indicates significant difference compared with the chlorogenic acid group, P < 0.05. # It indicates that there is a significant difference compared with the benzophenone-3 group, P < 0.05.

[0139] Experimental summary of antioxidant effect:

[0140] From the experimental results, we can see that sunflower seed meal extract has certain antioxidant activity. Although its activity is not as strong as chlorogenic acid, it is far superior to the existing chemically synthesized UV absorber benzophenone-3.

[0141] It can be seen that the present invention provides a sunflower seed meal extract and a preparation method and application thereof. The sunflower seed meal extract contains 6 phenolic acid compounds and 2 oligosaccharides, the total content of the 6 phenolic acid compounds is more than 17wt%, the total content of the 2 oligosaccharides is more than 33wt%, and the sum of the contents of the 6 phenolic acid compounds and the 2 oligosaccharides in the extract is greater than 50wt%; due to the reasonable composition ratio of the 6 phenolic acid compounds and the 2 oligosaccharides, the sunflower seed meal extract does not need to be added with a stabilizer or embedded in nanoliposomes to ensure the stability of the sunflower seed meal extract; the cytotoxicity of the sunflower seed meal extract is very low, and the 6 phenolic acid compounds and the 2 oligosaccharides have a synergistic effect of resisting ultraviolet radiation damage; the sunflower seed meal extract of the present invention is a high-efficiency and low-toxic natural ultraviolet absorber, has good resistance to ultraviolet radiation damage and a certain antioxidant effect, and can be used in sunscreen and antioxidant cosmetics and topical medicines; due to the instability of chlorogenic acid itself, the extraction High temperature, strong light and long-term heating are not allowed. In order to ensure the yield of chlorogenic acid itself and avoid oxidation of chlorogenic acid, when preparing the sunflower seed meal extract in the present application, the sunflower seed meal is first crushed and then subjected to segmented oscillation treatment, which can effectively destroy the cell wall of the sunflower seed meal, help the dissolution of the six phenolic acid compounds and the two oligosaccharides, and improve the extraction rate of chlorogenic acid, so that the total content of the six phenolic acid compounds in the extracted sunflower seed meal extract is above 17wt%, the total content of the two oligosaccharides is above 33wt%, and the sum of the contents of the six phenolic acid compounds and the two oligosaccharides in the extract is greater than 50wt%; due to the reasonable composition ratio of the six phenolic acid compounds and the two oligosaccharides, the stability of the sunflower seed meal extract is guaranteed, and the six phenolic acid compounds and the two oligosaccharides have a synergistic effect of resisting ultraviolet radiation damage, have good resistance to ultraviolet radiation damage and certain antioxidant effects, and can be applied to sunscreen and antioxidant cosmetics and external medicines; the present invention has the advantages of simple preparation method and low production cost.

[0142] The invention discloses a sunflower seed meal extract and a preparation method and application thereof, which also include an operation of promoting the stability of six phenolic acid compounds in the sunflower seed meal. Biogas slurry and intermittent supplementary light are applied before the sunflower seeds are harvested. Microorganisms secrete a variety of active substances when decomposing and fermenting biogas slurry gas-producing raw materials. Under the joint action of light and the various active substances and trace elements in the biogas slurry, the synthesis and accumulation of the six phenolic acid compounds in the sunflower seeds can be promoted, and the stability of the generated six phenolic acid compounds can be effectively improved. 15-20 g / L of potassium humate is also added to the biogas slurry, which can assist in promoting the synthesis and accumulation of raffinose in the sunflower seeds, and further ensure the stability of the sunflower seed meal extract.

[0143] The foregoing description of specific exemplary embodiments of the present invention is for the purpose of illustration and demonstration. These descriptions are not intended to limit the present invention to the precise form disclosed, and it is clear that many changes and variations can be made based on the above teachings. The purpose of selecting and describing the exemplary embodiments is to explain the specific principles of the present invention and its practical application, so that those skilled in the art can realize and utilize various different exemplary embodiments of the present invention and various different selections and changes. The scope of the present invention is intended to be limited by the claims and their equivalents.

Claims

1. A sunflower seed meal extract, characterized in that The sunflower seed meal extract includes 6 phenolic acid compounds and 2 oligosaccharides; wherein the 6 phenolic acid compounds are chlorogenic acid, neochlorogenic acid, cryptochlorogenic acid, isochlorogenic acid A, isochlorogenic acid B, and isochlorogenic acid C; and the 2 oligosaccharides are sucrose and raffinose; In the sunflower seed meal extract, the total content of the six phenolic acid compounds is 17.00-17.20 wt%, and the total content of the two oligosaccharides is 33.60-34.20 wt%; the content of each component in the sunflower seed meal extract is: chlorogenic acid content is 8.80-8.85 wt%, neochlorogenic acid content is 3.10-3.15 wt%, cryptochlorogenic acid content is 4.40-4.47 wt%, isochlorogenic acid A content is 0.15-0.18 wt%, isochlorogenic acid B content is 0.20-0.25 wt%, isochlorogenic acid C content is 0.28-0.31 wt%, sucrose content is 22.10-22.55 wt%, and raffinose content is 11.50-11.65 wt%. wt%; the ability of the sunflower seed meal extract to resist ultraviolet radiation damage is the result of the synergistic effect of the six phenolic acid compounds and the two oligosaccharides. The ability of the sunflower seed meal extract to resist ultraviolet radiation damage is equivalent to that of high-concentration chlorogenic acid, and is superior to that of the chemically synthesized ultraviolet absorber benzophenone-3; The preparation method of the sunflower seed meal extract comprises the following steps: (1) taking sunflower seed meal and crushing it into 200-300 meshes to obtain sunflower seed meal powder; (2) shaking the sunflower seed meal powder at a frequency of 35 to 45 times / second and a temperature of 5 to 10°C for 30 to 40 minutes to obtain a sunflower seed meal powder that has been shaken once; shaking the sunflower seed meal powder that has been shaken once for 25 to 30 minutes at a frequency of 50 to 55 times / second and a temperature of 5 to 10°C to obtain a sunflower seed meal powder that has been shaken twice; shaking the sunflower seed meal powder that has been shaken twice for 20 to 25 minutes at a frequency of 25 to 30 times / second and a temperature of 5 to 10°C to obtain a sunflower seed meal powder that has been shaken three times; (3) extracting six phenolic acid compounds and two oligosaccharides from the sunflower seed meal powder shaken three times with an ethanol-water solution, filtering and obtaining an extract; (4) Concentrating and drying the extract to obtain the sunflower seed meal extract.

2. A sunflower seed meal extract according to claim 1, characterized in that The step (1) also includes an operation for promoting the stability of the six phenolic acid compounds in the sunflower seed meal, and the operation method is as follows: 1) one month before the sunflower seeds are harvested, 300-400 kg / mu of biogas slurry is applied every 3-5 days, and the sunflower plants are supplemented with light every night, and the supplementary light process parameters are: intermittent supplementary light, turn on the light for supplementary light for 1 hour, turn off the light for 30 minutes, turn on the light for supplementary light for 1 hour, and then turn off the light for 30 minutes, and repeat this process until the supplementary light time reaches 4-5 hours; the number of supplementary light lamps per acre of sunflower is 200-250, the supplementary light lamp is 20 cm away from the sunflower plant, and the power of the supplementary light lamp is 25W; 2) harvest the sunflowers and put the sunflowers in trays for drying to obtain sunflower seeds; 3) take the sunflower seed oil to obtain the sunflower seed meal.

3. A sunflower seed meal extract according to claim 2, characterized in that The step (1) also includes an operation to promote the stability of the six phenolic acid compounds in the sunflower seed meal, and the operation method is as follows: 1) one month before the sunflower seeds are harvested, 350-400 kg / mu of biogas slurry is applied every 4-5 days, and the sunflower plants are supplemented with light every night, and the supplementary light process parameters are: intermittent supplementary light, turn on the light for supplementary light for 1 hour, turn off the light for 30 minutes, turn on the light for supplementary light for 1 hour, and then turn off the light for 30 minutes, and repeat this until the supplementary light time reaches 4-5 hours; the number of supplementary light lamps per acre of sunflower is 230-250, the supplementary light lamp is 20 cm away from the sunflower plant, and the power of the supplementary light lamp is 25W; 2) harvest the sunflowers and put the sunflowers into trays for airing and drying to obtain sunflower seeds; 3) take the sunflower seed oil to obtain the sunflower seed meal.

4. A sunflower seed meal extract according to claim 2 or 3, characterized in that: In the step (1), 15-20 g / L of potassium humate is also added to the biogas slurry.

5. The sunflower seed meal extract according to claim 1, characterized in that In the step (2), the sunflower seed meal powder is shaken for 35 to 40 minutes at a frequency of 40 to 45 times / second and a temperature of 8 to 10°C to obtain a sunflower seed meal powder that has been shaken once; the sunflower seed meal powder that has been shaken once is shaken for 27 to 30 minutes at a frequency of 52 to 55 times / second and a temperature of 8 to 10°C to obtain a sunflower seed meal powder that has been shaken twice; the sunflower seed meal powder that has been shaken twice is shaken for 22 to 25 minutes at a frequency of 28 to 30 times / second and a temperature of 8 to 10°C to obtain a sunflower seed meal powder that has been shaken three times.

6. The method for preparing a sunflower seed meal extract according to claim 1, characterized in that: The volume fraction of ethanol in the ethanol aqueous solution in step (3) is between 20% and 95%. The method for extracting the sunflower seed meal powder after three shakes is reflux extraction, ultrasonic extraction or supercritical fluid extraction.

7. The use of a sunflower seed meal extract according to any one of claims 1 to 6, characterized in that: It is used to prepare sunscreen cosmetics or sunscreen external medicines.

8. The use of a sunflower seed meal extract according to any one of claims 1 to 6, characterized in that: It is used to prepare antioxidant cosmetics or antioxidant external medicines.

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