A preparation process for fish scale collagen peptides

Abstract

The present invention provides a kind of preparation technology of fish scale collagen peptide, and fish scale cleaning is carried out in a cleaning device; the cleaning device includes a body, and a mesh cleaning basket is installed in the body; the cleaning basket is rotatably connected with the body; a cleaning structure is provided on the inner side of the cleaning basket; an aerator is installed at the inner bottom of the body; crushing, using a pulverizer to control the particle size of the fish scale powder between 0.1-0.25mm; enzyme extraction, alkaline protease, 4.7-5.5u / g, peptidase, 7.0-8.0u / g, enzymolysis time is 4-5h, enzymolysis temperature is 35-40℃, and solution pH is 6.5-7.0. The preparation technology of the present invention has an extraction rate of up to 92.7% for collagen; in the obtained product, the collagen peptide with a relative molecular weight of 1000-3000Da accounts for between 80-85%; the preparation technology has the advantages of being simple, easy to control, and suitable for promotion and application.

Description

Technical Field

[0001] The invention relates to the technical field of collagen peptide preparation, and in particular to a preparation process of fish scale collagen peptide. Background Art

[0002] Collagen is a functional protein, and its main source is animal tissues from poultry and livestock. As people's understanding of the function of collagen gradually improves, the demand for collagen is also gradually increasing, causing the extraction of collagen to develop from terrestrial organisms to marine organisms. Collagen has good affinity, biocompatibility and safety, making it widely used in the medical field; its moisturizing and emulsifying properties make it also widely used in the cosmetics field.

[0003] Collagen peptides are the hydrolysis products of collagen, and the relative molecular mass of the collagen peptides obtained by hydrolysis is less than 10,000 Da. Compared with collagen, collagen peptides are more easily absorbed and utilized by the human body, promoting intestinal digestion, promoting skin absorption, and increasing skin elasticity. Due to the advantage of collagen peptides being easily absorbed and utilized, their demand is increasing year by year.

[0004] With the rapid development of fisheries, the output of fish is increasing, but fish scales, as waste of fish, are rarely processed and utilized. Fish scales are rich in collagen and hydroxyapatite, and the collagen in them can be used in the fields of food, cosmetics and biomedicine. Therefore, when they are discarded, it causes a waste of resources. It can be seen that the extraction of collagen from fish scales to increase the added value of fish scales is of great significance to the industry.

[0005] The extraction methods of collagen peptides mainly include acid method, alkali method, enzyme method, neutral salt extraction method and hot water extraction method; when collagen comes into contact with acid solution, neutral salt solution, alkali solution and protease solution, they will act to break the cross-linking bonds of collagen and generate free peptide chains, which are beneficial to human body absorption and utilization when used. The acid method and subtraction method are two relatively extreme extraction methods. After extraction, the waste liquid needs to be treated to avoid harm to the environment; the enzyme method is a method with mild conditions, the obtained collagen peptides are highly active, and there is no pollution during the extraction process, which is more in line with the requirements of green production.

[0006] However, in the actual production process, there are many constraints on enzymatic extraction, including: differences in enzymatic raw materials, which make the enzymatic process also very different. Taking fish scales as an example, there is a large amount of mucus attached to the surface of fish scales. The presence of mucus affects the contact between enzymes and fish scales in the subsequent enzymatic hydrolysis process, thereby affecting the enzymatic hydrolysis effect; in the enzymatic hydrolysis process, improper control of the enzymatic hydrolysis time can easily produce bitter collagen peptides or insufficient extraction; improper control of the enzymatic hydrolysis temperature can easily lead to denaturation of collagen peptides and reduce biological activity; improper enzyme selection affects the extraction efficiency and biological activity of the product, etc. Therefore, providing a production process suitable for the extraction of collagen peptides from fish scales is of great significance to the fishery field. Summary of the invention

[0007] In view of the above-mentioned deficiencies in the prior art, the present invention provides a preparation process for fish scale collagen peptides, which includes fish scale cleaning, crushing, enzyme extraction, and drying; fish scale cleaning is carried out in a cleaning device; crushing is carried out using a pulverizer to control the particle size of the fish scale powder between 0.1-0.25mm; enzyme extraction, alkaline protease, 4.7-5.5u / g, peptidase, 7.0-8.0u / g, enzymolysis time is 4-5h, enzymolysis temperature is 35-40℃, and solution pH is 6.5-7.0. The preparation process of the present invention has an extraction rate of collagen of up to 92.7%; in the obtained product, the proportion of collagen peptides with a relative molecular weight of 1000-3000Da is between 80-85%; the preparation process has the advantages of being simple, easy to control, and suitable for popularization and application.

[0008] The technical solution of the present invention is as follows:

[0009] A preparation process of fish scale collagen peptide, comprising fish scale cleaning, crushing, enzyme extraction and drying;

[0010] Fish scale cleaning is carried out in the cleaning device; the use of cleaning equipment can effectively remove contaminants such as mucus and blood on the surface of fish scales to ensure the effect of subsequent treatment;

[0011] The cleaning device comprises a body, in which a mesh cleaning basket is installed; the cleaning basket is rotatably connected to the body through a rotating assembly; this arrangement enables the cleaning basket to rotate within the body, which is beneficial for removing moisture from the surface of the fish scales after cleaning;

[0012] A cleaning structure is provided on the inner side of the cleaning basket, and the cleaning structure is rotatably connected to the cleaning basket;

[0013] An aerator is installed at the inner bottom of the body, and the aerator is located between the cleaning basket and the body; the setting of the aerator can promote the flow of cleaning water during the cleaning process of fish scales, thereby driving the fish scales to move in the water, which is beneficial for water to penetrate between the adhered fish scales, and improve the cleanliness and cleaning efficiency of cleaning; in addition, when the fish scales are dried after cleaning, the gas sprayed by the aerator causes the fish scales to turn over in the cleaning basket, which is beneficial for the drying of the fish scales; the aerator is connected to a blower through an aeration pipeline;

[0014] Fish scales are rich in collagen, which is slightly soluble in cold water. When fish scales are damaged, collagen is easily lost. Therefore, the present invention adopts an aerator to turn the fish scales in water for cleaning. When the aerator sprays air, the liquid flow drives the fish scales to turn over and drives the cleaning structure to rotate. When the cleaning structure rotates, a certain force is generated on the surface of the fish scales. Under the premise of maximally avoiding damage to the fish scales, damage to the fish scales is avoided, thereby reducing the loss of collagen.

[0015] Crushing, using a crusher to control the particle size of the fish scale powder to be between 0.1-0.25 mm;

[0016] Enzyme extraction: alkaline protease, 4.7-5.5u / g, peptidase, 7.0-8.0u / g, enzymatic hydrolysis time is 4-5h, enzymatic hydrolysis temperature is 35-40℃, and solution pH is 6.5-7.0.

[0017] Preferably, the rotating assembly includes a motor, which is located outside the body and fixed to the bottom surface of the body. A connecting rod is fixed to the bottom of the cleaning basket, and the output shaft of the motor extends into the body and is detachably connected to the connecting rod.

[0018] Preferably, a groove A is provided on the free end surface of the output shaft, and the connecting rod extends into the groove A; two grooves B are symmetrically provided on the side of the groove A; a through groove is provided on the connecting rod, and a baffle is fixed in the through groove, the baffle divides the through groove into a first area and a second area, springs are fixed on both sides of the baffle, and locking blocks are fixed at the free ends of the springs, and the two locking blocks are respectively placed in the two grooves B, so that the cleaning basket and the rotating assembly become a whole; when the motor is running, the cleaning basket is driven to rotate.

[0019] Preferably, the groove B is close to the top of the groove A, and the through groove is close to the bottom end of the connecting rod; the depth h of the groove A is greater than the length s of the connecting rod; when the connecting rod extends into the groove A, there is a gap between the bottom end of the connecting rod and the inner bottom surface of the groove A. In addition, the depth of the groove A can satisfy the requirement that the locking block is completely disengaged from the groove B when the cleaning basket is pressed down. When the cleaning basket needs to be disassembled, the cleaning basket is pressed down to disengage the locking block from the groove B, and then the cleaning basket is rotated and lifted up.

[0020] Preferably, the longitudinal section of the locking block is a right-angled trapezoid, and the shape of the groove B matches the shape of the locking block; this arrangement can not only ensure the tightness of the locking, but also facilitate the disassembly of the cleaning basket, and has the advantages of reasonable design and strong practicality.

[0021] Preferably, in order to further improve the drying speed of the cleaned fish scales, a jet plate is provided on the inner side of the main body, and the jet plate is located between the cleaning basket and the main body; a jet head is provided on the jet plate; a heating component is provided on the air inlet pipe of the jet plate, and the heating component heats the gas entering the jet plate. When the aerator blows the fish scales to turn them over, the hot air sprayed from the jet head cooperates with the aerator to heat and dry the fish scales, thereby effectively improving the drying speed of the fish scales and reducing drying dead corners.

[0022] Preferably, an inverted U-shaped hook is fixed on the edge of the cleaning basket, one end of the inverted U-shaped hook is fixedly connected to the top surface of the cleaning basket, and the other end is located outside the main body; an arc groove is provided on the vertical section located outside the main body, and a rolling ball is placed in the arc groove, and the rolling ball contacts the outer side surface of the main body; when the cleaning basket rotates driven by the motor, the rolling ball can limit the cleaning basket, and can also avoid rigid friction and reduce equipment loss.

[0023] Preferably, in order to prevent fish scales from spilling during cleaning and drying, an annular groove is provided on the inner side of the cleaning basket, the annular groove being close to the top of the cleaning basket; an interception net is installed in the arc groove, which is beneficial to gas discharge during the drying process and can prevent fish scales from spilling.

[0024] Preferably, during the enzyme extraction process, the fish scale powder is mixed with water in a mass ratio of 20-30:100, the pH is adjusted to 6.7 with sodium bicarbonate, and then 5.2u / g of alkaline protease and 7.7u / g of peptidase are added, and enzymolysis is carried out at 35°C for 4.5h.

[0025] Preferably, the drying is performed by freeze drying.

[0026] Compared with the prior art, the beneficial effects of the present invention are:

[0027] 1. In the preparation process of the present invention, the fish scales are effectively cleaned to avoid damage to the fish scales and thus prevent loss of collagen during the cleaning stage, thereby increasing the extraction rate of collagen peptides.

[0028] 2. The preparation process of the present invention has an extraction rate of collagen as high as 92.7%; in the obtained product, the proportion of collagen peptides with a relative molecular weight of 1000-3000Da is between 80-85%.

[0029] 3. The preparation process of the present invention is simple, easy to control, and suitable for popularization and application. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, for ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0031] Figure 1 It is a schematic diagram of the structure of the cleaning device.

[0032] Figure 2 This is an enlarged view of the M section.

[0033] In the figure, 1-main body, 2-cleaning basket, 3-hook, 4-arc groove, 5-roller ball, 6-motor, 7-connecting rod, 8-output shaft, 9-groove A, 10-groove B, 11-through groove, 12-baffle, 13-spring, 14-locking block, 15-bearing, 16-rotating shaft, 17-cleaning brush, 18-aerator, 19-jet plate, 20-jet head, 21-heating component, 22-annular groove, 23-interception net, 24-discharge port, 25-valve. DETAILED DESCRIPTION

[0034] In order to enable those skilled in the art to better understand the technical solutions in the present invention, the technical solutions in the embodiments of the present invention will be clearly and completely described below in combination with the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work should fall within the scope of protection of the present invention.

[0035] Example 1

[0036] Combination Figure 1 and Figure 2 The present invention provides a cleaning device for use in a fish scale collagen peptide preparation process, comprising a main body 1, in which a mesh cleaning basket 2 is installed; the cleaning basket 2 is rotatably connected to the main body 1 through a rotating assembly; this arrangement enables the cleaning basket 2 to rotate within the main body 1, which is beneficial for removing moisture from the surface of the fish scale after cleaning;

[0037] An inverted U-shaped hook 3 is fixed on the side of the cleaning basket 2, one end of the inverted U-shaped hook 3 is fixedly connected to the top surface of the cleaning basket 2, and the other end is located outside the body 1; an arc groove 4 is provided on the vertical section located outside the body 1, and a rolling ball 5 is placed in the arc groove 4, and the rolling ball 5 is in contact with the outer side of the body 1; when the cleaning basket 2 rotates driven by the motor 6, the rolling ball 5 can limit the cleaning basket 2, reduce the left and right shaking of the cleaning basket 2 as much as possible, and can also avoid rigid friction and reduce equipment loss;

[0038] The rotating assembly includes a motor 6, which is located outside the body 1 and fixed to the bottom surface of the body 1. A connecting rod 7 is fixed to the bottom of the cleaning basket 2. The output shaft 8 of the motor 6 extends into the body 1 and is detachably connected to the connecting rod 7.

[0039] The specific structure of the detachable connection is:

[0040] A groove A9 is provided on the free end surface of the output shaft 8, and the connecting rod 7 extends into the groove A9; two grooves B10 are symmetrically provided on the side of the groove A9; a through groove 11 is provided on the connecting rod 7, and a baffle 12 is fixed in the through groove 11, and the baffle 12 divides the through groove 11 into a first area and a second area, and springs 13 are fixed on both sides of the baffle 12, and the two springs 13 are respectively located in the first area and the second area, and the free ends of the springs 13 are fixed with locking blocks 14, and the two locking blocks 14 are respectively placed in the two grooves B10, so that the cleaning basket 2 and the rotating assembly become a whole; when the motor 6 is running, the cleaning basket 2 is driven to rotate;

[0041] The groove B10 is close to the top of the groove A9, and the through groove 11 is close to the bottom of the connecting rod 7; the depth h of the groove A9 is greater than the length s of the connecting rod 7; when the connecting rod 7 extends into the groove A9, there is a gap between the bottom of the connecting rod 7 and the bottom surface of the groove A9. When the cleaning basket 2 needs to be disassembled, the cleaning basket 2 is pressed down to make the locking block 14 disengage from the groove B10, and then the cleaning basket 2 is rotated and lifted upward;

[0042] The longitudinal section of the locking block 14 is a right-angled trapezoid, and the shape of the groove B10 matches the shape of the locking block 14. This arrangement can not only ensure the tightness of the locking, but also facilitate the disassembly of the cleaning basket 2, and has the advantages of reasonable design and strong practicality.

[0043] A cleaning structure is provided on the inner side of the cleaning basket 2, and the cleaning structure is rotatably connected to the cleaning basket 2;

[0044] Specifically, a bearing 15 is fixed on the inner side of the cleaning basket 2, a rotating shaft 16 is fixed in the bearing 15, and a cleaning brush 17 is sleeved on the outer side of the rotating shaft 16; there is no relative movement between the cleaning brush 17 and the rotating shaft 16; the bristles of the cleaning brush 17 are made of one of silicone material, rubber material or plastic material, and these types of bristles can clean the fish scales to a certain extent without damaging the fish scales; in this embodiment, rubber bristles are used;

[0045] An aerator 18 is installed at the inner bottom of the body 1, and the aerator 18 is located between the cleaning basket 2 and the body 1; the setting of the aerator 18 can promote the flow of cleaning water during the cleaning process of the fish scales, thereby driving the fish scales to move in the water, which is beneficial for water to penetrate between the adhered fish scales, and improve the cleanliness and cleaning efficiency of the cleaning; in addition, when the fish scales are dried after cleaning, the gas sprayed by the aerator 18 causes the fish scales to turn over in the cleaning basket 2, which is beneficial for the drying of the fish scales; the aerator 18 is connected to a blower (not shown in the figure) through an aeration pipeline;

[0046] In order to further improve the drying speed of the cleaned fish scales, a jet plate 19 is provided on the inner side of the body 1, and the jet plate 19 is located between the cleaning basket 2 and the body 1; a jet head 20 is provided on the jet plate 19; a heating component 21 is provided on the air inlet pipe of the jet plate 19. In this embodiment, the heating component 21 uses an electric heating wire, and the heating component 21 heats the gas entering the jet plate 19. When the aerator 18 blows the fish scales to turn them over, the hot air sprayed from the jet head 20 cooperates with it to heat and dry the fish scales, effectively improving the drying speed of the fish scales and reducing the drying dead angle;

[0047] In order to prevent the fish scales from spilling during the cleaning and drying process, an annular groove 22 is provided on the inner side of the cleaning basket 2, and the annular groove 22 is close to the top of the cleaning basket 2; an interception net 23 is installed in the arc groove 4, and the interception net 23 is conducive to the gas discharge during the drying process and can prevent the fish scales from spilling;

[0048] The bottom plate of the body 1 is arranged in an inclined shape, and a discharge port 24 is provided at the lowest position of the bottom plate, and a valve 25 is installed at the discharge port 24 to facilitate the discharge of waste liquid;

[0049] Fish scales are rich in collagen, which is slightly soluble in cold water. When fish scales are damaged, collagen is easily lost. Therefore, the present invention adopts an aerator 18 to make the fish scales flip over in water for cleaning. When the aerator 18 sprays air, the liquid flow drives the fish scales to flip over and drives the cleaning structure to rotate. When the cleaning structure rotates, a certain force is exerted on the surface of the fish scales. Under the premise of maximally avoiding damage to the fish scales, damage to the fish scales is avoided, thereby reducing the loss of collagen.

[0050] The fish scales after cleaning were observed to have no adherent impurities or dry mucus on the surface of the fish scales, and the scales were well dispersed; the overall integrity of the fish scales was the same as when they were put in, that is, the use of the cleaning device can achieve the purpose of effective cleaning while keeping the fish scales intact.

[0051] Example 2

[0052] A preparation process of fish scale collagen peptide, the specific steps are as follows:

[0053] (1) Fish scale cleaning:

[0054] The cleaning device provided in Example 1 was used to clean the scales of grass carp, and the intact scales accounted for more than 95% of the total scales. The impurities such as mucus and blood stains on the surface of the scales were removed to obtain dry scales, which were weighed. The moisture content in the scales was determined to be 8.75%. The moisture content was determined by direct drying method according to GB 5009.3-1985.

[0055] (2) Crushing:

[0056] Take the fish scales obtained in step (1) and grind them using a pulverizer to obtain fish scale powder with a particle size of 0.15-0.25 mm;

[0057] The particle size of fish scale powder has an important influence on the effect of enzyme extraction; if the particle size is too small, it is easy to produce the disadvantage of too fast enzymatic hydrolysis and poor controllability, and it is very easy to generate bitter small molecule peptides. If the particle size is too large, it is easy to lead to long enzymatic hydrolysis time and low hydrolysis rate.

[0058] (3) Enzyme extraction:

[0059] The fish scale powder was mixed with water in a mass ratio of 25:100, and after mixing, the pH was adjusted to 6.7 with sodium bicarbonate, and then 5.2u / g of alkaline protease and 7.7u / g of peptidase were added, and enzymolysis was performed at 35°C for 4.5h to obtain an enzymolysis solution;

[0060] (4) Drying:

[0061] The enzymatic hydrolysate is centrifuged to collect the supernatant; the supernatant is concentrated and freeze-dried to obtain a finished product;

[0062] Using hydroxyproline as a control, the protein content in the supernatant was determined and the collagen extraction rate was calculated to be 92.7%. Using the size exclusion method, the proportion of collagen peptides with a relative molecular weight between 1000-3000Da was 84.9%.

[0063] The collagen content is equal to the hydroxyproline content multiplied by a coefficient of 11.1; the collagen extraction rate is calculated according to the following formula:

[0064]

[0065] Where, m1 is the mass of hydroxyproline measured, g;

[0066] m2 is the sampling mass of the sample during measurement, g;

[0067]

[0068] Where, M1 is the mass of the finished product, g;

[0069] M2 is the mass of fish scale, g.

[0070] Example 3

[0071] A preparation process of fish scale collagen peptide, the specific steps are as follows:

[0072] (1) Fish scale cleaning:

[0073] The cleaning device provided in Example 1 was used to clean the scales of grass carp, and impurities such as mucus and blood stains on the surface of the scales were removed to obtain dry scales. The water content in the scales was measured, and the water content was 8.51%;

[0074] (2) Crushing:

[0075] A pulverizer is used to obtain fish scale powder with a particle size of 0.1-0.2 mm;

[0076] (3) Enzyme extraction:

[0077] The fish scale powder and water were mixed at a mass ratio of 20:100, and after mixing, the pH was adjusted to 6.5 with sodium bicarbonate, and then 4.7u / g of alkaline protease and 7.0u / g of peptidase were added, and enzymolysis was performed at 38°C for 5h to obtain an enzymolysis solution;

[0078] (4) Drying:

[0079] The enzymatic hydrolysate is centrifuged to collect the supernatant; the supernatant is concentrated and freeze-dried to obtain a finished product;

[0080] Using hydroxyproline as a reference, the protein content in the supernatant was determined and the collagen extraction rate was calculated, which was 91.6%; the collagen peptides with a relative molecular weight between 1000-3000Da accounted for 84.1%.

[0081] Example 4

[0082] A preparation process of fish scale collagen peptide, the specific steps are as follows:

[0083] (1) Fish scale cleaning:

[0084] The cleaning device provided in Example 1 was used to clean grass carp scales, and impurities such as mucus and blood stains on the scale surface were removed to obtain dry scales. The water content in the scales was measured, and the water content was 8.35%;

[0085] (2) Crushing:

[0086] A pulverizer is used to obtain fish scale powder with a particle size of 0.2-0.25 mm;

[0087] (3) Enzyme extraction:

[0088] The fish scale powder and water were mixed at a mass ratio of 30:100, and after mixing, the pH was adjusted to 7.0 with sodium bicarbonate, and then 5.5u / g of alkaline protease and 8.0u / g of peptidase were added, and enzymolysis was performed at 40°C for 5h to obtain an enzymolysis solution;

[0089] (4) Drying:

[0090] The enzymatic hydrolysate is centrifuged to collect the supernatant; the supernatant is concentrated and freeze-dried to obtain a finished product;

[0091] Using hydroxyproline as a reference, the protein content in the supernatant was determined and the collagen extraction rate was calculated, which was 91.1%; the collagen peptides with a relative molecular weight between 1000-3000Da accounted for 84.5%.

[0092] Example 5

[0093] The difference from Example 2 is that in the enzyme extraction process of step (3), the enzymatic hydrolysis is carried out at 35° C. for 5 h to obtain an enzymatic hydrolyzate.

[0094] The collagen extraction rate is 92.0%; the proportion of collagen peptides with a relative molecular weight between 1000-3000Da is 84.3%.

[0095] Comparative Example 1

[0096] The difference from Example 2 is that in step (2), the particle size of the fish scale powder obtained after crushing is less than 0.15 mm;

[0097] The collagen extraction rate is 89.8%; the proportion of collagen peptides with a relative molecular weight between 1000-3000Da is 88.3%.

[0098] Comparative Example 2

[0099] The difference from Example 2 is that in step (2), the particle size of the fish scale powder obtained after crushing is 0.3-0.6 mm;

[0100] The collagen extraction rate is 76.5%; the proportion of collagen peptides with a relative molecular weight between 1000-3000Da is 80.3%.

[0101] Comparative Example 3

[0102] The difference from Example 2 is that in step (3), alkaline protease 6.0u / g, peptidase 9.0u / g, enzymolysis at 40°C for 5h to obtain an enzymolysis solution;

[0103] The collagen extraction rate is 89.9%.

[0104] Comparative Example 4

[0105] The difference from Example 2 is that in step (3), the pH is 6.3;

[0106] The collagen extraction rate is 88.4%.

[0107] Comparative Example 5

[0108] Taking fish scales, wherein broken fish scales account for more than 85% of the total amount, and cleaning them using the cleaning device of Example 1 to obtain dried fish scales;

[0109] Then, the dried fish scales having the same weight as that in Example 2 were taken, and collagen peptides were extracted using the same method as in Example 2, and the extraction rate of collagen was calculated;

[0110] The results were: collagen extraction rate was 80.1%; collagen peptides with relative molecular weight between 1000-3000Da accounted for 84.4%.

[0111] It can be seen from Examples 2-5 that the preparation process provided by the present invention has the advantages of high extraction efficiency and short preparation time for the extraction of collagen from fish scales, and among the collagen obtained by this method, collagen peptides with a relative molecular weight between 1000-3000Da account for about 84%, and the collagen peptides within this molecular weight range are easier to absorb and utilize; in addition, the preparation process provided by the present invention has a stable process and the obtained product quality consistency is high.

[0112] It can be seen from Example 2 and Comparative Example 5 that as the cleaning process proceeds, factors such as the temperature of the solution and the cleaning time may cause the protein in the broken fish scales to dissolve in the water, resulting in protein loss, thereby affecting the extraction rate.

[0113] Although the present invention has been described in detail by reference to the preferred embodiments, the present invention is not limited thereto. Without departing from the spirit and essence of the present invention, a person of ordinary skill in the art may make various equivalent modifications or substitutions to the embodiments of the present invention, and these modifications or substitutions shall be within the scope of the present invention. Any person of ordinary skill in the art may easily think of changes or substitutions within the technical scope disclosed by the present invention, and these shall be within the scope of protection of the present invention. Therefore, the scope of protection of the present invention shall be subject to the scope of protection of the claims.

Claims

1. A process for preparing fish scale collagen peptide, comprising cleaning, crushing, enzyme extraction and drying of fish scales; characterized in that: Fish scale cleaning is carried out in a cleaning device; The cleaning device comprises a body, in which a mesh cleaning basket is installed; the cleaning basket is rotatably connected to the body through a rotating assembly; A cleaning structure is provided on the inner side of the cleaning basket, and the cleaning structure is rotatably connected to the cleaning basket; An aerator is installed at the inner bottom of the body, and the aerator is located between the cleaning basket and the body; the aerator is connected to a blower through an aeration pipeline; Crushing, using a crusher to control the particle size of the fish scale powder to be between 0.1-0.25 mm; Enzyme extraction: mix fish scale powder and water in a mass ratio of 20-30:100, adjust the pH to 6.5-7.0 with sodium bicarbonate; alkaline protease, 4.7-5.5u / g, peptidase, 7.0-8.0u / g, enzymolysis time is 4-5h, and enzymolysis temperature is 35-40℃.

2. The process for preparing fish scale collagen peptide according to claim 1, characterized in that: The rotating assembly comprises a motor, which is located outside the body and fixed on the bottom surface of the body. A connecting rod is fixed on the bottom of the cleaning basket, and the output shaft of the motor extends into the body and is detachably connected to the connecting rod.

3. The process for preparing fish scale collagen peptide according to claim 2, characterized in that: A groove A is provided on the free end surface of the output shaft, and the connecting rod extends into the groove A; two grooves B are symmetrically provided on the side of the groove A; a through groove is provided on the connecting rod, and a baffle is fixed in the through groove, and the baffle divides the through groove into a first area and a second area, and springs are fixed on both sides of the baffle, and locking blocks are fixed at the free ends of the springs, and the two locking blocks are respectively placed in the two grooves B.

4. The process for preparing fish scale collagen peptide according to claim 3, characterized in that: The groove B is close to the top of the groove A, and the through groove is close to the bottom of the connecting rod; the depth h of the groove A is greater than the length s of the connecting rod, and the depth of the groove A can meet the requirement that the locking block is completely separated from the groove B when the cleaning basket is pressed down.

5. The process for preparing fish scale collagen peptide according to claim 4, characterized in that: The longitudinal section of the locking block is a right-angled trapezoid, and the shape of the groove B matches the shape of the locking block.

6. The process for preparing fish scale collagen peptide according to claim 1, characterized in that: An air jet plate is arranged on the inner side of the main body, and the air jet plate is located between the cleaning basket and the main body; an air jet head is arranged on the air jet plate; and a heating component is arranged on the air inlet pipe of the air jet plate.

7. The process for preparing fish scale collagen peptide according to claim 1, characterized in that: An inverted U-shaped hook is fixed on the edge of the cleaning basket, one end of the inverted U-shaped hook is fixedly connected to the top surface of the cleaning basket, and the other end is located outside the body; an arc groove is provided on the vertical section located outside the body, a rolling ball is placed in the arc groove, and the rolling ball contacts the outer side surface of the body.

8. The process for preparing fish scale collagen peptide according to claim 1, characterized in that: An annular groove is arranged on the inner side surface of the cleaning basket, and the annular groove is close to the top of the cleaning basket; an interception net is installed in the arc groove.

9. The process for preparing fish scale collagen peptide according to claim 1, characterized in that: During the enzyme extraction process, sodium bicarbonate was used to adjust the pH to 6.7, and then 5.2u / g of alkaline protease and 7.7u / g of peptidase were added, and enzymolysis was carried out at 35°C for 4.5h.

10. The process for preparing fish scale collagen peptide according to claim 1, characterized in that: The drying is carried out by freeze drying.

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