Immunohistochemical staining machine

Abstract

The present invention provides an immunohistochemical staining machine, comprising a machine body, and a slide rack, a liquid immersion mechanism, a Y-axis moving component, a liquid removal chamber, a reagent adding mechanism, an incubation mechanism, a bidirectional clamping mechanism and a first rotating table installed in the machine body. Through the periodic rotation of the first rotating table, after the first rotating table drives the bidirectional clamping mechanism to rotate, two clamping members can be aligned with the slide rack and the reagent adding mechanism at the same time, or respectively aligned with the liquid removal chamber and the incubation mechanism at the same time, and the two clamping members can both realize the operation of clamping and placing the slide, and the bidirectional clamping mechanism can drive the two clamping members to move closer to or away from each other, so that each clamping member can send the clamped slide into the corresponding mechanism, and the experimental operation steps do not interfere with each other. In this way, the transportation stroke and number of slides are reduced, which is more efficient than the efficiency of repeated transportation of a single slide in conventional immunohistochemical staining equipment, and greatly simplifies the operation process of immunohistochemistry.

Description

Technical Field

[0001] The invention relates to the technical field of sample staining equipment, in particular to an immunohistochemical staining machine. Background Art

[0002] Immunohistochemical staining is an application of the reaction principle of specific binding between antigen and antibody in immunology. It generally uses biotin-labeled antibodies to display antigens in tissues. It is generally used clinically for genetic testing of malignant tumors.

[0003] The operation steps of immunohistochemistry generally include: tissue dewaxing and hydration, antigen repair, blocking endogenous catalase, antibody hybridization, color development, counterstaining, dehydration and sealing. During the whole operation process, the slides are washed in PBS buffer for many times, and the excess PBS solution must be shaken off after washing. Then, different reagents must be added to each slide several times. The whole operation process is not only time-consuming, but also complicated.

[0004] Although there are corresponding immunohistochemical staining machines now, the existing staining machines can generally only clamp a single slide for cleaning, reagent dripping and staining operations, and the transportation stroke and number of times for a single slide are relatively large, and the transportation efficiency is low. Summary of the invention

[0005] In view of the defects in the prior art, the purpose of the present invention is to provide an immunohistochemical staining machine to simplify the operation process of immunohistochemistry and improve the experimental efficiency.

[0006] In order to achieve the above-mentioned purpose, the present invention provides an immunohistochemical staining machine, comprising a slide rack, which is provided with a plurality of first insertion positions for inserting slides, and the plurality of first insertion positions are arranged along the Y-axis direction; an immersion mechanism, which is used to clean or stain the slides in the slide rack; a Y-axis moving component, which is used to synchronously drive the slide rack and the immersion mechanism to perform lifting and lowering actions along the Y-axis direction; a liquid removal chamber, which is used to remove excess liquid on the slide; a reagent adding mechanism, which is arranged opposite to the slide rack, and the reagent adding mechanism is used to add reagents to the slide; an incubation mechanism, which is arranged opposite to the liquid removal chamber, and the incubation mechanism is used to incubate the slide; a bidirectional clamping mechanism, which has two clamping members that can clamp the slide, the clamping ends of the two clamping members are arranged back to back, and the two clamping members can synchronously move closer to or away from each other; and a first rotating table, which is used to drive the bidirectional clamping mechanism to rotate so that the two clamping members are respectively aligned with the slide rack and the reagent adding mechanism, or respectively aligned with the liquid removal chamber and the incubation mechanism.

[0007] Preferably, the immersion mechanism comprises a second rotating table, a plurality of liquid storage tanks and a rotating unit, the second rotating table is arranged on the Y-axis moving assembly, the rotation axis of the second rotating table is parallel to the Y-axis direction, the plurality of liquid storage tanks are arranged on the second rotating table and arranged in a circular array along the rotation axis of the second rotating table, and the second rotating table can drive any one of the liquid storage tanks to move to the bottom of the slide rack;

[0008] The slide rack has a pick-and-place state and an immersion state, and the rotating unit is used to drive the slide rack to rotate to the pick-and-place state or the immersion state. When the slide rack is in the pick-and-place state, multiple first insertion positions are arranged along the Y-axis direction, and the clamping member can clamp the slide in the first insertion position; when the slide rack is in the immersion state, the slide rack is located in the liquid storage tank, and multiple first insertion positions are arranged along the X-axis direction.

[0009] Preferably, the rotating unit includes: a first bracket; a rotating shaft, which is rotatably engaged with the first bracket, and the rotating shaft is arranged along the Z-axis direction; a first swing arm, which has an "L"-shaped structure, one end of the first swing arm is fixedly mounted on the rotating shaft, and the other end of the first swing arm is connected to the wafer carrier; and a first motor, which is arranged on the first bracket and is transmission-connected to the rotating shaft.

[0010] Preferably, the immersion mechanism also includes a Z-axis moving component and a second bracket, the Z-axis moving component is arranged on the Y-axis moving component and is located above the second rotating table, and the Z-axis moving component is used to drive the second bracket to move along the Z-axis direction; the wafer carrier and the rotating unit are each provided in plurality, and the plurality of wafer carriers and the plurality of rotating units are arranged on the second bracket along the Z-axis direction.

[0011] Preferably, the bidirectional clamping mechanism includes: a third bracket, which is arranged on the first rotating table; a screw, which is rotatably engaged with the third bracket, and the screw has a first threaded portion and a second threaded portion with opposite thread rotation directions; two nut seats, which are respectively threadedly engaged with the first threaded portion and the second threaded portion, and the nut seats are slidably engaged with the third bracket in a direction parallel to the axis of the screw, and the two clamping members are respectively arranged on a corresponding one of the nut seats; and a second motor, which is arranged on the third bracket and is transmission-connected to the screw.

[0012] Preferably, the second motor is arranged in the middle part of the screw, the first threaded portion and the second threaded portion are respectively arranged on both sides of the second motor, and the third bracket is provided with a mounting groove for installing the second motor; the third bracket is provided with two guide holes, and the guide holes extend in a direction parallel to the axis of the screw, and a guide block is provided at the bottom of the nut seat, and each guide block slides in the corresponding guide hole.

[0013] Preferably, a second insertion position is provided on the side of the liquid removal bin facing the incubation mechanism, and an air inlet channel, a liquid discharge channel and a collection chamber are provided in the liquid removal bin. The air inlet channel is provided at the top of the second insertion position and is connected with the second insertion position, and the liquid discharge channel is respectively connected with the second insertion position and the collection chamber.

[0014] Preferably, the incubation mechanism includes an incubation chamber and an XY-axis moving component, a plurality of incubation units are provided on the side of the incubation chamber facing the liquid removal chamber, the plurality of incubation units are arranged at intervals along the X-axis direction, and each of the incubation units includes a plurality of third insertion positions arranged along the Y-axis direction; the XY-axis moving component is used to drive the incubation chamber to move along the X-axis or Y-axis direction so that one of the third insertion positions is aligned with the second insertion position.

[0015] Preferably, the reagent adding mechanism includes: a second swing arm; a liquid adding instrument, which is arranged on the second swing arm; a third motor, which is used to drive the second swing arm to rotate; a YZ-axis moving component, which is provided with a fourth bracket connected to the third motor, and the YZ-axis moving component is used to drive the liquid adding instrument to move along the Y-axis or Z-axis direction; a reagent rack, which is provided with a plurality of reagent bottles, and the plurality of reagent bottles are arranged along the Z-axis direction, and the liquid adding instrument can be extended into the reagent bottles; and a cleaning chamber, which is provided on the reagent rack, and the cleaning chamber is used to clean the liquid adding instrument.

[0016] Beneficial effects of the present invention:

[0017] The present invention discloses an immunohistochemical staining machine, which is periodically rotated by a first rotating table. After the first rotating table drives a bidirectional clamping mechanism to rotate, two clamping members can be aligned with a slide rack and a reagent adding mechanism at the same time, or respectively aligned with a liquid removal bin and an incubation mechanism at the same time. Both clamping members can realize the operation of clamping and placing a slide, and the bidirectional clamping mechanism can drive the two clamping members to move closer to or away from each other. Therefore, each clamping member can send the clamped slide into the corresponding mechanism, and the experimental operation steps do not interfere with each other. In this way, the transportation stroke and number of slides are reduced, which is more efficient than the efficiency of repeated transportation of a single slide in conventional immunohistochemical staining equipment, greatly simplifies the operation process of immunohistochemistry, and improves experimental efficiency. At the same time, the error factor of manual operation is also removed, the shortcomings of unstable quality and poor effect of manual staining are overcome, and the staining quality is improved. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the following is a brief introduction to the drawings required for the specific embodiments or the description of the prior art. In all the drawings, similar elements or parts are generally identified by similar reference numerals. In the drawings, the elements or parts are not necessarily drawn according to the actual scale.

[0019] Figure 1 A schematic diagram of the structure of an immunohistochemical staining machine provided in one embodiment of the present invention;

[0020] Figure 2 A schematic diagram of the structure in which two clamping members are aligned with the slide rack and the reagent adding mechanism respectively;

[0021] Figure 3 It is a structural schematic diagram of the immersion mechanism;

[0022] Figure 4 It is a structural schematic diagram of the cooperation between the slide rack and the rotating unit (the slide rack is in the pick-up and place state);

[0023] Figure 5 It is a schematic diagram of the structure of the slide holder when it is in a liquid immersion state;

[0024] Figure 6 It is a structural schematic diagram of the deliquescence tank;

[0025] Figure 7 It is a structural schematic diagram of the reagent adding mechanism;

[0026] Reference numerals:

[0027] 10- Slide rack;

[0028] 20-liquid immersion mechanism, 21-second rotating platform, 22-liquid storage tank, 23-rotating unit, 231-first bracket, 232-rotating shaft, 233-first swing arm, 234-first motor, 24-Z-axis moving assembly, 25-second bracket;

[0029] 30-Y-axis moving assembly;

[0030] 40 - liquid removal chamber, 41 - second insertion position, 42 - air inlet channel, 43 - liquid discharge channel, 44 - collection chamber;

[0031] 50-reagent adding mechanism, 51-second swing arm, 52-liquid adding instrument, 53-third motor, 54-YZ axis moving assembly, 55-reagent rack, 56-reagent bottle, 57-cleaning chamber;

[0032] 60-incubation mechanism, 61-incubation chamber, 62-XY axis moving component;

[0033] 70-bidirectional clamping mechanism, 71-clamping member, 72-third bracket, 73-screw, 75-second motor;

[0034] 80-first rotating table;

[0035] 90-body. DETAILED DESCRIPTION

[0036] The following embodiments of the technical solution of the present invention are described in detail in conjunction with the accompanying drawings. The following embodiments are only used to more clearly illustrate the technical solution of the present invention, and are therefore only used as examples, and cannot be used to limit the protection scope of the present invention.

[0037] It should be noted that, unless otherwise specified, the technical terms or scientific terms used in this application should have the common meanings understood by those skilled in the art to which the present invention belongs.

[0038] In the description of the present application, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", "axial", "radial", "circumferential" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as limiting the present invention.

[0039] In addition, the terms "first", "second", etc. are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. In the description of the present invention, the meaning of "plurality" is more than two, unless otherwise clearly and specifically defined.

[0040] In this application, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0041] In the present application, unless otherwise clearly specified and limited, a first feature being “above” or “below” a second feature may mean that the first and second features are in direct contact, or the first and second features are in indirect contact through an intermediate medium. Moreover, a first feature being “above”, “above”, and “above” a second feature may mean that the first feature is directly above or obliquely above the second feature, or simply means that the first feature is higher in level than the second feature. A first feature being “below”, “below”, and “below” a second feature may mean that the first feature is directly below or obliquely below the second feature, or simply means that the first feature is lower in level than the second feature.

[0042] like Figure 1-7 As shown, in one embodiment of the present invention, an immunohistochemical staining machine is provided, comprising a machine body 90, and a slide rack 10 installed in the machine body 90, a liquid immersion mechanism 20, a Y-axis moving assembly 30, a liquid removal chamber 40, a reagent adding mechanism 50, an incubation mechanism 60, a bidirectional clamping mechanism 70 and a first rotating table 80. Figure 1 , the directions of the three coordinate axes in the figure represent the X-axis direction, Y-axis direction and Z-axis direction below respectively.

[0043] A plurality of first insertion positions for inserting glass slides are provided on one side of the slide rack 10 facing the reagent adding mechanism 50, and the plurality of first insertion positions are arranged along the Y-axis direction. The immersion mechanism 20 is used to clean or stain the glass slides in the slide rack 10. The immersion mechanism 20 is filled with solutions such as xylene solution, anhydrous ethanol, tap water, distilled water, H2O2 solution, PBS buffer solution, and hematoxylin solution. The Y-axis moving component 30 is used to synchronously drive the slide rack 10 and the immersion mechanism 20 to perform lifting and lowering movements along the Y-axis direction. The Y-axis moving component 30 is a prior art and will not be described in detail in this embodiment.

[0044] The liquid removal chamber 40 is used to remove excess liquid on the slide, the reagent adding mechanism 50 is arranged opposite to the slide rack 10, and the reagent adding mechanism 50 is used to add reagent to the slide. The incubation mechanism 60 is arranged opposite to the liquid removal chamber 40, and the incubation mechanism 60 is used to incubate the slide.

[0045] The bidirectional clamping mechanism 70 has two clamping members 71 for clamping a glass slide, which are respectively referred to as clamping member 71a and clamping member 71b for ease of description. The clamping ends of clamping member 71a and clamping member 71b are arranged opposite to each other, and the bidirectional clamping mechanism 70 synchronously drives clamping member 71a and clamping member 71b to move closer to or away from each other. The first rotating platform 80 is used to drive the bidirectional clamping mechanism 70 to rotate so that clamping member 71a and clamping member 71b are respectively aligned with the slide rack 10 and the reagent adding mechanism 50, or respectively aligned with the liquid removal chamber 40 and the incubation mechanism 60.

[0046] Among them, the immersion mechanism 20, Y-axis moving assembly 30, liquid removal chamber 40, reagent adding mechanism 50, incubation mechanism 60, bidirectional clamping mechanism 70 and first rotating table 80 are all controlled by a controller (not shown in the drawings). The controller can be a PLC, etc., which will not be repeated here.

[0047] The working principle of the immunohistochemical staining machine is as follows:

[0048] The operator first inserts the slide glass that has undergone antigen repair into the first insertion position of the slide rack 10 from top to bottom in sequence, and the immunohistochemical staining machine starts working. The immersion mechanism 20 first drives the slide rack 10 to be immersed in the corresponding solution in sequence. Then, the clamping member 71a clamps a slide glass on the slide rack 10 and takes it out from the first insertion position, and the first rotating table 80 drives the bidirectional clamping mechanism 70 to rotate a certain angle (in this embodiment, the rotation angle of the first rotating table 80 when working is ninety degrees). At this time, the clamping member 71a is aligned with the liquid removal bin 40, and the clamping member 71b is aligned with the incubation mechanism 60. The clamping member 71a inserts the clamped slide glass into the liquid removal bin 40, and the liquid removal bin 40 removes the excess residual liquid (usually PBS solution) on the slide glass. At the same time, since the clamping member 71a will no longer affect the slide rack 10, the Y-axis moving component 30 can drive the slide rack 10 to rise, so that the next slide glass is in the position to be clamped.

[0049] Subsequently, the two-way clamping mechanism 70 drives the clamping member 71a and the clamping member 71b to move closer to each other, and the clamping member 71a will take the slide glass out of the liquid removal chamber 40. The first rotating platform 80 then drives the two-way clamping mechanism 70 to rotate ninety degrees. At this time, the clamping member 71a is aligned with the reagent adding mechanism 50, and the clamping member 71b is aligned with the slide holder 10. The two-way clamping mechanism 70 drives the clamping member 71a and the clamping member 71b to move away from each other, and the clamping member 71a will send the clamped slide glass into the reagent adding mechanism 50, and the reagent adding mechanism 50 will drip the corresponding reagent to the slide glass. At the same time, the clamping member 71b will clamp the slide glass in the slide holder 10. When the clamping member 71a and the clamping member 71b move closer to each other, the clamping member 71b can take the slide glass out of the slide holder 10, and the clamping member 71a will also take the slide glass dripped with the reagent out of the reagent adding mechanism 50.

[0050] Then, the first rotating platform 80 drives the bidirectional clamping mechanism 70 to rotate 90 degrees. At this time, the clamping member 71a is aligned with the incubation mechanism 60, and the clamping member 71b is aligned with the liquid removal chamber 40. The bidirectional clamping mechanism 70 drives the clamping member 71a and the clamping member 71b to move away from each other. The clamping member 71a will send the clamped slide into the incubation mechanism 60 and release the slide, so that the slide is incubated in the incubation mechanism 60. At the same time, the clamping member 71b will insert the clamped slide into the liquid removal chamber 40 for liquid removal. At the same time, the Y-axis moving assembly 30 drives the slide rack 10 to rise, so that the next slide is in the position to be clamped.

[0051] When the clamping member 71a and the clamping member 71b of the two-way clamping mechanism 70 move toward each other, the clamping member 71b can take the glass slide out of the liquid removal chamber 40, and the first rotating platform 80 drives the two-way clamping mechanism 70 to rotate 90 degrees. At this time, the clamping member 71a is aligned with the slide rack 10 and can clamp the glass slide, while the clamping member 71b is aligned with the reagent adding mechanism 50. Repeat the above steps until all the glass slides in the slide rack 10 are sent to the incubation mechanism 60 for incubation.

[0052] Of course, it is understandable that the first rotating platform 80 can perform forward or reverse rotation, and the first rotating platform 80 can also be set to have an adjustable rotation angle.

[0053] The immunohistochemical staining machine provided in this embodiment is characterized in that the first rotating table 80 rotates periodically. After the first rotating table 80 drives the bidirectional clamping mechanism 70 to rotate, the two clamping members 71 can be aligned with the slide rack 10 and the reagent adding mechanism 50 at the same time, or respectively aligned with the liquid removal bin 40 and the incubation mechanism 60 at the same time. The two clamping members 71 can both realize the operation of clamping and placing the slide, and the bidirectional clamping mechanism 70 can drive the two clamping members 71 to move closer to or away from each other. Therefore, each clamping member 71 can send the clamped slide into the corresponding mechanism, and the experimental operation steps do not interfere with each other. In this way, the transportation stroke and number of slides are reduced, which is more efficient than the efficiency of repeated transportation of a single slide in conventional immunohistochemical staining equipment, greatly simplifying the operation process of immunohistochemistry and improving experimental efficiency. At the same time, it also eliminates the error factors of manual operation, overcomes the shortcomings of unstable quality and poor effect of manual staining, and improves the staining quality.

[0054] In one embodiment, the immersion mechanism 20 includes a second rotating table 21, a plurality of liquid storage tanks 22 and a rotating unit 23. The second rotating table 21 is disposed on the Y-axis moving assembly 30. The rotation axis 232 of the second rotating table 21 is parallel to the Y-axis direction. The plurality of liquid storage tanks 22 are disposed on the second rotating table 21 and are arranged in a circular array along the rotation axis 232 of the second rotating table 21. The second rotating table 21 can drive any liquid storage tank 22 to move to the bottom of the slide rack 10.

[0055] The slide rack 10 has a pick-and-place state and an immersion state. The rotating unit 23 is used to drive the slide rack 10 to rotate to the pick-and-place state or the immersion state. When the slide rack 10 is in the pick-and-place state, the multiple first insertion positions are arranged along the Y-axis direction, and the clamping member 71 can clamp the glass slide in the first insertion position; when the slide rack 10 is in the immersion state, the slide rack 10 is located in the liquid storage tank 22, and the multiple first insertion positions are arranged along the X-axis direction.

[0056] Each liquid reservoir 22 contains a corresponding solution. When the glass slide needs to be cleaned, the clamping member 71a and the clamping member 71b reinsert the glass slide into the first insertion position of the slide rack 10. Then, the second rotating platform 21 drives the corresponding liquid reservoir 22 to move to the bottom of the slide rack 10, and the rotating unit 23 drives the slide rack 10 to rotate from the pick-and-place state to the immersion state, and the glass slide in the slide rack 10 will be immersed in the solution for cleaning.

[0057] After cleaning, the rotating unit 23 drives the slide rack 10 to rotate from the immersion state to the placement state. With the cooperation of the first rotating table 80 and the bidirectional clamping mechanism 70, the slides in the slide rack 10 can be de-liquidated, reagents can be added and incubated.

[0058] The structural design of the immersion mechanism 20 and the slide rack 10 that cooperate with each other makes it unnecessary for the immunohistochemical staining machine to transport the slide rack 10. By driving the slide rack 10 to switch between the pick-up and placement state or the immersion state through the rotating unit 23, cleaning and staining work can be carried out, or the slides can be transported. In this way, the experimental operation process is simplified and the efficiency is improved.

[0059] In one embodiment, the rotating unit 23 includes a first bracket 231, a rotating shaft 232, a first swing arm 233 and a first motor 234. The rotating shaft 232 is rotatably matched on the first bracket 231. The rotating shaft 232 is arranged along the Z-axis direction. The first swing arm 233 is in an "L"-shaped structure. One end of the first swing arm 233 is fixedly sleeved on the rotating shaft 232, and the other end of the first swing arm 233 is connected to the slide rack 10. The first motor 234 is arranged on the first bracket 231 and is connected to the rotating shaft 232. The structural design of the first swing arm 233 enables the slide rack 10 to be immersed as deeply as possible in the liquid storage tank 22 during the process of switching from the pick-and-place state to the immersion state, so as to avoid mutual interference between the first swing arm 233 and the liquid storage tank 22. Of course, the slide rack 10 and the first swing arm 233 can be designed to be detachably connected, so that it is more convenient to load the repaired slide into the slide rack 10.

[0060] In one embodiment, the immersion mechanism 20 further includes a Z-axis moving assembly 24 and a second bracket 25. The Z-axis moving assembly 24 is disposed on the Y-axis moving assembly 30 and is located above the second rotating table 21. The Z-axis moving assembly 24 is used to drive the second bracket 25 to move along the Z-axis direction. Three slide racks 10 and three rotating units 23 are provided, and the three slide racks 10 and the three rotating units 23 are arranged on the second bracket 25 along the Z-axis direction. By designing multiple sets of slide racks 10 and rotating units 23, the processing capacity of the immunohistochemical staining machine is increased, thereby improving the work efficiency.

[0061] In one embodiment, the bidirectional clamping mechanism 70 includes a third bracket 72, a lead screw 73, two nut seats, and a second motor 75. The third bracket 72 is arranged on the first rotating platform 80. The lead screw 73 is rotatably matched with the third bracket 72. The lead screw 73 has a first threaded portion and a second threaded portion with opposite thread rotation directions. The second motor 75 is arranged on the third bracket 72 and is in transmission connection with the lead screw 73. The two nut seats are respectively threadedly matched with the first threaded portion and the second threaded portion. The nut seats are slidably matched with the third bracket 72 in a direction parallel to the axis of the lead screw 73. The two clamping members 71 are respectively arranged on a corresponding nut seat. When the second motor 75 drives the lead screw 73 to rotate, the two nut seats can be driven to drive the clamping members 71a and 71b to move closer to or away from each other.

[0062] In one embodiment, the second motor 75 is arranged in the middle of the lead screw 73, the first threaded portion and the second threaded portion are arranged on both sides of the second motor 75, and the third bracket 72 is provided with a mounting groove for mounting the second motor 75. The third bracket 72 is provided with two guide holes, which extend in a direction parallel to the axis of the lead screw 73. The bottom of the nut seat is provided with a guide block, and each guide block is slidably fitted in the corresponding guide hole. The position design of the second motor 75 reduces the occupied space of the bidirectional clamping mechanism 70, and avoids the second motor 75 from interfering with the slide rack 10, the liquid removal chamber 40, the reagent adding mechanism 50 and the incubation mechanism 60 during the rotation of the third bracket 72.

[0063] In one embodiment, a second insertion position 41 is provided on the side of the de-liquidation bin 40 facing the incubation mechanism 60, and an air inlet channel 42, a liquid discharge channel 43 and a collection chamber 44 are provided in the de-liquidation bin 40. The air inlet channel 42 is provided at the top of the second insertion position 41 and communicates with the second insertion position 41, and the liquid discharge channel 43 communicates with the second insertion position 41 and the collection chamber 44, respectively. An air supply device (not shown in the drawings) is communicated with the air inlet channel 42. After the glass slide is inserted into the second insertion position 41, the air supply device supplies compressed air of a certain pressure to the air inlet channel 42, so that the excess solution on the surface of the glass slide can be blown off, thereby effectively removing the excess solution on the surface of the glass slide, and the fallen solution enters the collection chamber 44 from the liquid discharge channel 43 for recovery.

[0064] In one embodiment, the incubation mechanism 60 includes an incubation chamber 61 and an XY axis moving assembly 62. Three incubation units are provided on the side of the incubation chamber 61 facing the liquid removal chamber 40. The three incubation units are arranged at intervals along the X axis direction, and each incubation unit includes a plurality of third insertion positions arranged along the Y axis direction. The XY axis moving assembly 62 is used to drive the incubation chamber 61 to move along the X axis or the Y axis direction so that one of the third insertion positions is aligned with the second insertion position 41.

[0065] The number of incubation units corresponds to the number of slide racks 10, and similarly, the number of third insertion positions in each incubation unit corresponds to the number of first insertion positions in each slide rack 10. After the glass slide clamped by the clamp 71a has completed the addition of reagent in the reagent adding mechanism 50, and the glass slide in the slide rack 10 is clamped by the clamp 71b, the second motor 75 drives the lead screw 73 to rotate, so that the clamp 71a and the clamp 71b move closer to each other, and then the first rotating platform 80 drives the third bracket 72 to rotate ninety degrees. At this time, the clamp 71a is aligned with the incubation chamber 61, and the clamp 71b is aligned with the liquid removal chamber 40.

[0066] The second motor 75 then drives the lead screw 73 to rotate, so that the clamping member 71a and the clamping member 71b move away from each other, and the clamping member 71a will insert the clamped glass slide into the third insertion position and release the glass slide, so that the glass slide is incubated in the incubation chamber 61. The clamping member 71b will insert the clamped glass slide into the second insertion position 41 and maintain the clamping state. Finally, the second motor 75 drives the clamping member 71a and the clamping member 71b to move closer to each other, and the first rotating platform 80 drives the third bracket 72 to rotate ninety degrees. At this time, the clamping member 71a will align with the slide rack 10, ready to clamp the glass slide in the slide rack 10, and the clamping member 71b will send the clamped glass slide into the reagent adding mechanism 50, and the reagent adding mechanism 50 will drip the corresponding reagent to the glass slide.

[0067] The incubation chamber 61 can perform operations such as heating, heat preservation, and moisturizing on the glass slide. The incubation chamber 61 and the XY axis moving component 62 are both existing technologies and will not be described in detail in this embodiment.

[0068] In one embodiment, the reagent adding mechanism 50 includes a second swing arm 51, a liquid adding instrument 52, a third motor 53, a YZ axis moving assembly 54, a reagent rack 55 and a cleaning chamber 57. The liquid adding instrument 52 is arranged on the second swing arm 51, the third motor 53 is used to drive the second swing arm 51 to rotate, the YZ axis moving assembly 54 is provided with a fourth bracket connected to the third motor 53, and the YZ axis moving assembly 54 is used to drive the liquid adding instrument 52 to move along the Y axis or Z axis. The reagent rack 55 is provided with a plurality of reagent bottles, and the plurality of reagent bottles are arranged along the Z axis direction. The liquid adding instrument 52 can extend into the reagent bottles, and the cleaning chamber 57 is arranged on the reagent rack 55, and the cleaning chamber 57 is used to clean the liquid adding instrument 52.

[0069] When the glass slide held by the clamping member 71a or the clamping member 71b is aligned with the reagent adding mechanism 50, the YZ axis moving assembly 54 drives the liquid adding device 52 to absorb the corresponding reagent, and the third motor 53 drives the second swing arm 51 to rotate, so that the liquid adding device 52 is located above the glass slide, and the absorbed reagent is dripped onto the glass slide. Then, the second motor 75 drives the clamping member 71a and the clamping member 71b to move closer to each other, and the first rotating platform 80 drives the third bracket 72 to rotate ninety degrees, so that the clamping member 71a or the clamping member 71b is aligned with the incubation chamber 61.

[0070] Then, the third motor 53 drives the second swing arm 51 to rotate 180 degrees, and the YZ axis moving assembly 54 drives the liquid adding device 52 to be inserted into the cleaning chamber 57 for cleaning, waiting for the next liquid adding operation. The liquid adding device 52, the cleaning chamber 57 and the YZ axis moving assembly 54 are all existing technologies and will not be described in detail in this embodiment.

[0071] In the description of the present invention, a large number of specific details are described. However, it is understood that embodiments of the present invention can be practiced without these specific details. In some instances, well-known methods, structures and techniques are not shown in detail so as not to obscure the understanding of this description.

[0072] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some or all of the technical features therein by equivalents. These modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of the technical solutions of the embodiments of the present invention, and they should all be included in the scope of the claims and specification of the present invention.

Claims

1. An immunohistochemical staining machine, characterized in that: include: A slide rack, which is provided with a plurality of first insertion positions for inserting glass slides, wherein the plurality of first insertion positions are arranged along the Y-axis direction; An immersion mechanism, which is used to clean or stain the glass slide in the slide rack; A Y-axis moving assembly, which is used to synchronously drive the slide holder and the immersion mechanism to perform lifting and lowering movements along the Y-axis direction; A liquid removal chamber, which is used to remove excess liquid on the slide; A reagent adding mechanism, which is arranged opposite to the slide holder and is used to add reagents to the slide; An incubation mechanism, which is arranged opposite to the liquid removal chamber and is used to incubate the slide glass; A bidirectional clamping mechanism, which has two clamping members capable of clamping a glass slide, the clamping ends of the two clamping members are arranged opposite to each other, and the two clamping members can move toward or away from each other synchronously; and A first rotating platform, which is used to drive the bidirectional clamping mechanism to rotate so that the two clamping members are respectively aligned with the slide rack and the reagent adding mechanism, or are respectively aligned with the liquid removal chamber and the incubation mechanism; The immersion mechanism includes a second rotating table, a plurality of liquid storage tanks and a rotating unit, wherein the second rotating table is arranged on the Y-axis moving assembly, the rotation axis of the second rotating table is parallel to the Y-axis direction, the plurality of liquid storage tanks are arranged on the second rotating table and arranged in a circular array along the rotation axis of the second rotating table, and the second rotating table can drive any one of the liquid storage tanks to move to the bottom of the slide rack; The slide rack has a pick-and-place state and an immersion state, and the rotating unit is used to drive the slide rack to rotate to the pick-and-place state or the immersion state. When the slide rack is in the pick-and-place state, multiple first insertion positions are arranged along the Y-axis direction, and the clamping member can clamp the slide in the first insertion position; when the slide rack is in the immersion state, the slide rack is located in the liquid storage tank, and multiple first insertion positions are arranged along the X-axis direction.

2. The immunohistochemical staining machine according to claim 1, characterized in that: The rotating unit comprises: First bracket; A rotating shaft, which is rotatably engaged with the first bracket, and the rotating shaft is arranged along the Z-axis direction; A first swing arm, which is in an "L"-shaped structure, one end of the first swing arm is fixedly sleeved on the rotating shaft, and the other end of the first swing arm is connected to the slide holder; and A first motor is disposed on the first bracket and is drivingly connected to the rotating shaft.

3. The immunohistochemical staining machine according to claim 2, characterized in that: The immersion mechanism further includes a Z-axis moving assembly and a second bracket, wherein the Z-axis moving assembly is disposed on the Y-axis moving assembly and is located above the second rotating table, and the Z-axis moving assembly is used to drive the second bracket to move along the Z-axis direction; A plurality of the wafer carriers and the plurality of rotating units are provided, and the plurality of wafer carriers and the plurality of rotating units are arranged on the second bracket along the Z-axis direction.

4. The immunohistochemical staining machine according to any one of claims 1 to 3, characterized in that: The bidirectional clamping mechanism comprises: A third bracket, which is arranged on the first rotating platform; A lead screw, which is rotatably engaged with the third bracket, and has a first threaded portion and a second threaded portion with opposite thread rotation directions; two nut seats, respectively threadedly engaged with the first threaded portion and the second threaded portion, the nut seats slidingly engaged with the third bracket in a direction parallel to the axis of the lead screw, the two clamping members respectively disposed on a corresponding one of the nut seats; and The second motor is arranged on the third bracket and is drivingly connected to the lead screw.

5. The immunohistochemical staining machine according to claim 4, characterized in that: The second motor is arranged in the middle of the lead screw, the first threaded portion and the second threaded portion are arranged on both sides of the second motor respectively, and the third bracket is provided with a mounting groove for mounting the second motor; The third bracket is provided with two guide holes, and the guide holes extend in a direction parallel to the axis of the lead screw. A guide block is provided at the bottom of the nut seat, and each guide block is slidably fitted in the corresponding guide hole.

6. The immunohistochemical staining machine according to claim 1, characterized in that: A second insertion position is provided on the side of the liquid removal bin facing the incubation mechanism, and an air inlet channel, a liquid discharge channel and a collection chamber are provided in the liquid removal bin. The air inlet channel is provided at the top of the second insertion position and is connected with the second insertion position, and the liquid discharge channel is respectively connected with the second insertion position and the collection chamber.

7. The immunohistochemical staining machine according to claim 6, characterized in that: The incubation mechanism comprises an incubation chamber and an XY axis moving assembly, a plurality of incubation units are arranged on one side of the incubation chamber facing the liquid removal chamber, the plurality of incubation units are arranged at intervals along the X axis direction, and each of the incubation units comprises a plurality of third insertion positions arranged along the Y axis direction; The XY axis moving component is used to drive the incubation chamber to move along the X axis or Y axis direction so that one of the third insertion positions is aligned with the second insertion position.

8. The immunohistochemical staining machine according to claim 1, characterized in that: The reagent adding mechanism comprises: Second swing arm; a liquid adding device, which is arranged on the second swing arm; a third motor, used for driving the second swing arm to rotate; A YZ axis moving component, which is provided with a fourth bracket connected to the third motor, and the YZ axis moving component is used to drive the liquid adding instrument to move along the Y axis or Z axis direction; A reagent rack, which is provided with a plurality of reagent bottles, wherein the plurality of reagent bottles are arranged along the Z-axis direction, and the liquid adding instrument can extend into the reagent bottles; and A cleaning chamber is arranged on the reagent rack, and the cleaning chamber is used to clean the liquid adding instrument.

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