Automated pipetting apparatus and method

Abstract

An automated pipetting apparatus includes a dispensing head having at least one pipette tip mandrel (200) having an elongated dowel body (300) with an insertion end. The at least one pipette tip mandrel cooperates with a pipette tip (399). A first adjustable seal (310A) disposed on the elongated dowel body and a second adjustable seal (310B) disposed on the elongated dowel body seal the pipette tip that cooperates to the at least one pipette tip mandrel. The first adjustable seal defines a cushion surface. The cushion surface enables a substantially continuous circumferential contact seal with radial tight contact between the pipette tip and the at least one pipette tip mandrel. The second adjustable seal is adjustable between a disengaged position and an engaged position and enables a releasable grip and another substantially continuous circumferential contact seal around the pipette tip between the pipette tip and the at least one pipette tip mandrel.

Description

Technical Field

[0001] Exemplary embodiments generally relate to automated liquid handling and dispensing, and more particularly to apparatus and methods for automated liquid transfer. Background Technology

[0002] In general, disposable pipette tips used with automated liquid handling and dispensing systems enable the reuse of such pipetting systems to transfer different fluid samples without cross-contamination. Disposable pipette tips generally have a hollow, elongated shape and an overall tapered shape. The upper end of the pipette tip typically includes a collar that is mounted to the pipette mandrel of the automated liquid handling and dispensing system.

[0003] In general, the disposable pipette tip is held on the pipette mandrel by a friction coupling created by a tapering fit between the pipette mandrel and the pipette collar, and a sealing ring on the inner circumference of the pipette collar, to secure and seal the pipette tip to the mounting shaft. The fit between the pipette mandrel and the disposable tip is achieved by pushing the pipette mandrel into the tapering pipette collar until the pipette mandrel is wedged into the pipette tip. A seal between the pipette tip and the pipette mandrel is formed between the pipette collar and the mounting shaft due to the compression of the sealing ring and / or the stretching of the collar diameter.

[0004] Automated liquid handling and dispensing systems generally have several pipette mandrels on a common dispensing head, allowing multiple samples to be transferred together in a single movement of the dispensing head. Mounting each disposable pipette on a mandrel generally requires significant force to ensure all pipettes are adequately attached to their respective mandrels on the common dispensing head. The force required to adequately attach the pipettes to the mandrels directly affects the size and cost of at least some parts of the automated liquid handling and dispensing system, as the actuators need to be sized to generate sufficient force to mount the pipettes onto the mandrels. Furthermore, the actuators need to have sufficient capacity to mount the pipettes onto the mandrels, for example, for continuous use over long periods. The structure of the automated liquid handling and dispensing system also needs to have sufficient rigidity so that structural deformation does not affect the mounting of the pipettes onto the mandrels.

[0005] It will be advantageous to have an automated liquid handling and dispensing system that utilizes sufficient force to load the pipette tip onto the corresponding pipette mandrel while keeping the size and cost of the automated liquid handling and dispensing system to a minimum. Summary of the Invention

[0006] An automated pipetting device, comprising: A dispensing head having at least one pipette mandrel, the pipette mandrel having an elongated pin body having an insertion end, the at least one pipette mandrel being configured to mate with a pipette, the elongated pin body of the at least one pipette mandrel being first inserted into the pipette with the insertion end; A first adjustable seal is disposed on the elongated pin body and configured such that the first adjustable seal seals the pipette, the pipette engaging with the at least one pipette mandrel, the first adjustable seal being positioned close to the insertion end; A second adjustable seal is disposed on the elongated pin body and configured such that the second adjustable seal seals the pipette, which engages with the at least one pipette mandrel, and the second adjustable seal is positioned remote from the insertion end; and The first adjustable seal defines a buffer surface for the at least one pipette mandrel. When in contact with the pipette and the buffer surface of the first adjustable seal, the buffer surface buffers the insertion of the at least one pipette mandrel into the pipette. The buffer surface is configured such that the buffer contact adjusts the first adjustable seal and wedges the first adjustable seal against the pipette. A continuous circumferential contact seal is achieved by utilizing the radially tight contact between the pipette and the at least one pipette mandrel. The second adjustable seal is adjustable between a non-engaged position and an engaged position. The non-engaged position provides a clearance fit between the at least one pipette mandrel and the pipette. In the engaged position, the second adjustable seal contacts the pipette, which engages with the at least one pipette mandrel, achieving a releasable grip and another continuous circumferential contact seal around the pipette between the pipette and the at least one pipette mandrel.

[0007] An automated pipetting method, comprising: A dispensing head is provided having at least one pipette mandrel, the pipette mandrel having an elongated pin body having an insertion end, the at least one pipette mandrel being configured to mate with a pipette, the elongated pin body of the at least one pipette mandrel being first inserted into the pipette with the insertion end; A first adjustable seal is provided on the elongated pin body and configured such that the first adjustable seal seals the pipette, the pipette engaging with the at least one pipette mandrel, the first adjustable seal being positioned close to the insertion end; A second adjustable seal is provided, which is disposed on the elongated pin body and configured such that the second adjustable seal seals the pipette, which engages with the at least one pipette mandrel, and the second adjustable seal is positioned away from the insertion end; By utilizing the buffer surface of the at least one pipette mandrel defined by the first adjustable seal, when the pipette and the buffer surface of the at least one pipette mandrel come into contact, insertion of the at least one pipette mandrel into the pipette is buffered, wherein the buffer contact of the buffer surface adjusts the first adjustable seal and causes the first adjustable seal to wedge against the pipette, and continuous circumferential contact seal is achieved by utilizing the radial tight contact between the pipette and the at least one pipette mandrel; and The second adjustable seal is adjusted between a non-engaged position and an engaged position, wherein the non-engaged position provides a clearance fit between the at least one pipette mandrel and the pipette, and in the engaged position, the second adjustable seal contacts the pipette, which engages with the at least one pipette mandrel, thereby achieving a releasable grip and another continuous circumferential contact seal around the pipette between the pipette and the at least one pipette mandrel.

[0008] A pipette tip for an automated pipetting device, the pipette tip comprising: A pipette collar, having an insertion end, is configured to intersect and automatically engage with the pipette mandrel of the automated pipetting device; A dispensing nozzle extends from the pipette collar and is opposite the insertion end; A first sealing stop surface is disposed on the surface of the pipette collar. The first sealing stop surface is configured to engage and cushion the insertion of the pipette spindle, such that the cushioned contact between the first sealing stop surfaces causes a first sealing portion of the pipette spindle to wedge against the first sealing stop surface. A continuous circumferential contact seal is achieved through the radially tight contact between the first sealing stop surface and the pipette spindle. A second sealing stop surface is disposed on the surface of the pipette collar such that the second sealing stop surface is longitudinally spaced from the first sealing stop surface. The second sealing stop surface is configured to engage and cooperate with the second sealing portion of the pipette spindle, thereby achieving a releasable grip and another continuous circumferential contact seal between the second sealing stop surface and the pipette spindle. Attached Figure Description

[0009] The foregoing aspects and other features of the disclosed embodiments are explained in the following description in conjunction with the accompanying drawings, wherein: Figure 1 This is a schematic diagram of an automated pipetting apparatus according to aspects of the disclosed embodiments; Figure 1A This is a schematic diagram of an automated pipetting apparatus according to aspects of the disclosed embodiments; Figure 2 This is a schematic diagram of a portion of an automated pipetting apparatus according to aspects of the disclosed embodiments; Figure 2A This is a schematic diagram of a portion of an automated pipetting apparatus according to aspects of the disclosed embodiments; Figure 3 This is a schematic diagram of a portion of an automated pipetting apparatus according to aspects of the disclosed embodiments; Figure 4 This is a schematic diagram of a portion of an automated pipetting apparatus according to aspects of the disclosed embodiments; Figure 5A and Figure 5B This is a schematic diagram of the junction between a portion of an automated pipetting device and a disposable pipette tip, according to an aspect of the disclosed embodiment; Figure 5C This is a schematic diagram of the junction between a portion of an automated pipetting device and a disposable pipette tip, according to an aspect of the disclosed embodiment; Figure 6A and Figure 6B This is a schematic diagram of the junction between a portion of an automated pipetting device and a disposable pipette tip, according to an aspect of the disclosed embodiment; Figure 7 This is a flowchart based on aspects of the disclosed embodiments; Figure 8 This is a flowchart based on an aspect of the disclosed embodiments; Figure 9 This is a schematic diagram of a pipette according to an aspect of the disclosed embodiments; Figures 10A-10C This is a schematic diagram of a pipette according to an aspect of the disclosed embodiments; and Figure 11A-11C This is a schematic diagram of a pipette according to an aspect of the disclosed embodiments. Detailed Implementation

[0010] Figure 1 and Figure 1A This is a schematic diagram of an automated pipetting apparatus according to aspects of the disclosed embodiments. Although aspects of the disclosed embodiments will be described with reference to the accompanying drawings, it should be understood that aspects of the disclosed embodiments can be implemented in many forms. Furthermore, elements or materials of any suitable size, shape, or type can be used. Moreover, although aspects of the disclosed embodiments have been described herein with respect to automated liquid handling and dispensing systems, aspects of the disclosed embodiments are equally applicable to manually operated pipetting apparatuses.

[0011] refer to Figure 1 , Figure 1A and Figure 2 The disclosed embodiments provide an automated pipetting device 100 having a dispensing head 130, which applies minimal force to engage and disengage pipette tips from the dispensing head 130. The automated pipetting device 100 is configured to simultaneously capture / grip and seal each pipette (attached to each pipette by multiple seals) to the dispensing head 130, and utilizes a common actuation action from a common motor / actuator 110M of the automated pipetting device 100, wherein the simultaneous capture and sealing act substantially simultaneously on the tip holder RCK (pipette holding tray / cart). Figure 3 All pipettes in the device. In one aspect, the pipette holder RCK has a standard pipette holding capacity, such as, for example, 96 pipette tips or 384 pipette tips, while in other aspects, the pipette holder RCK may have any suitable pipette holding capacity. As will be described in more detail below, minimal force can be achieved by providing a slight gap between the pipette tip and the pipette spindle 200-200n. The gap between the pipette tip and the pipette spindle 200-200n allows the pipette spindle 200-200n to slide into the pipette tip with minimal force. The pipette tip is pushed against a first sealing surface of the pipette spindle 200-200n, which sets or defines the position of the pipette tip relative to the pipette spindle 200-200n. Using an expandable component / seal disposed on the pipette mandrel 200-200n, the pipette is positioned in a predetermined alignment relative to the pipette mandrel 200-200n and held on the pipette mandrel 200-200n, wherein the expandable component / seal engages with and grips the flat or tapered inner surface of the pipette. Friction between the expandable component / seal and the pipette holds the pipette on the pipette mandrel 200-200n.

[0012] like Figure 1As can be seen, the automated pipetting device 100 includes a frame 101. Any suitable transport device 120 (such as, for example, a gantry transport system 120A, a robotic arm 10B, or a combination thereof) can be connected to the frame 101. The automated pipetting device 100 also includes a drive section 110 connected to the transport device 120. A dispensing head 130 can be mounted to and carried by the transport device 120, thereby connected to the frame 101, wherein movement of the transport device 120 and thus the dispensing head 130 is provided by the drive section 110. Any suitable controller 150 can be connected to the drive section 110 and includes any suitable programs and memory for implementing the operation of the automated pipetting device 100 as described herein. In one aspect, the automated pipetting device 100 may be substantially similar to a mobile vehicle described in U.S. Patent Application 14 / 636,962, filed March 3, 2015 and published as Early Publication No. 2016 / 0256866 on September 8, 2016, which may in turn be included in a robotic processing system substantially similar to that described in U.S. Provisional Application 62 / 381,400, filed August 30, 2016, entitled “Robotic Processing System”, the disclosure of which is incorporated herein by reference in its entirety. In other aspects, the automated pipetting device may have any suitable mobile or stationary configuration.

[0013] Also refer to Figure 1A In one aspect, the automated pipetting apparatus 100 may include a cart 100MC. The cart 100MC may include a pipetting chamber 1400, a storage chamber 1460 coupled to the pipetting chamber 1400, and a control system 1480 (which may be any suitable control system with a processor, such as controller 150, configured to perform any suitable operation of the automated pipetting apparatus 100). Although the pipetting chamber 1400 and the storage chamber 1460 are shown as being included in a common housing or frame (such as frame 101), in other aspects, the pipetting chamber 1400 may be separated from the storage chamber 1460, such that the pipetting chamber 1400 can be used as a separate pipetting chamber. In one aspect, the pipetting chamber 1400 may be accessed through one or more doors 1412, wherein the pipetting chamber 1400 is sealed relative to the surrounding environment when one or more doors 1412 are closed. The storage chamber 1460 may also be similarly sealed relative to the surrounding environment by one or more doors 1412.

[0014] In one aspect, the pipetting chamber 1400 may include a set of pipette cartridges 1414, which in one aspect include a dispensing head 130, the pipette cartridges 1414 docking at any suitable docking position in the pipetting chamber 1400. The pipetting chamber 1400 may also include at least one tray docking station 1416 for holding pipetting trays 1418. The pipetting chamber 1400 may include any suitable carrier 1424, which in one aspect is substantially similar to the transport device 120, the carrier 1424 being configured to transport each of the pipette cartridges 1414 to a pipetting position, for example, at the position of at least one of the pipetting trays 1418 within the tray docking station 1416 in the pipetting chamber 1400. In one aspect, the carrier 1424 is a gantry system, while in other aspects, the carrier may be any suitable transport device, such as an articulated robotic transport arm. The mobile vehicle 100MC may also include any suitable robotic transport arm 1422 configured to move the pipette 1418 between the pipetting chamber 1400 and the storage rotary table 1449 of the storage chamber 1416.

[0015] refer to Figure 2 The dispensing head 130 includes a frame 205 having a first member 205A, a second member 205B, and one or more guide members 207 (such as guide rods) that connect the first member 205A and the second member 205B at a predetermined interval, such that the first member 205A and the second member 205B are separated from each other by a predetermined distance. In one aspect, the dispensing head 130 also includes a plate 206 disposed between the first member 205A and the second member 205B, wherein the plate 206 is movably coupled to the frame 205. In one aspect, the plate 206 is movably coupled to one or more guide members 207 such that one of the frame 205 and the plate 206 is movable relative to the other of the frame 205 and the plate 206 in a direction 299. In one aspect, wherein the frame 205 is fixedly coupled to the transport device 120, and the plate 206 is movable relative to the frame 205 in a direction 299 for gripping and releasing the pipette tip, as described herein. On the other hand, plate 206 may be fixedly coupled to transport device 120 such that frame 205 moves relative to plate 206 in direction 299 for gripping and releasing pipettes, as described herein.

[0016] refer to Figure 2 and Figure 2AThe relative movement or travel between frame 205 and plate 206 can be achieved by the pivoting action of one or more connecting rods 210. In one aspect, each of the one or more connecting rods 210 includes a longitudinally extending body 210B having a first end 210E1 and a second end 210E2. The first end 210E1 is rotatably coupled to a second member 205B of the frame about a corresponding pivot axis AXL, wherein the corresponding pivot axis AXL extends from opposite sides of the second member 205B along a common axis AX1. The second end 210E2 of each of the one or more connecting rods 210 is connected to a drive motor 110M of the drive section 110, as will be described in further detail below.

[0017] Each of one or more connecting rods 210 includes a protruding member 210SA disposed between a first end 210E1 and a second end 210E2 of the respective connecting rod 210. In one aspect, the protruding member 210SA includes a pin 210SAS extending laterally from the respective connecting rod 210 and a bushing 210SAB mating with the pin 210SAS; in other aspects, the protruding member may be a single component, coupled to the respective connecting rod 210 in any suitable manner, or formed as a single construction with the respective connecting rod 210. The protruding member 210SA engages an elongated groove 220 in the plate 206, wherein the longitudinal axis 220X of the elongated groove 220 is arranged laterally to the direction 299 of relative movement between the frame 205 and the plate 206. In one aspect, the longitudinal axis 220X extends in direction 297. In one aspect, the protruding member 210SA is dimensioned such that there is a clearance fit between the protruding member 210SA and the elongated slot 220, such that as one or more connecting rods 210 pivot about a corresponding pivot axis AXL in direction 298 (e.g., about axis AX1), the protruding member 210SA moves within the elongated slot 220 along the longitudinal axis 220X, and the intersection between the protruding member 210SA and the longitudinal sides 220L1, 220L2 of the elongated slot 220 results in relative movement between the plate 206 and the frame 205 in direction 299, wherein the amount D of relative movement is at least partially defined by the distance of the arc AR traveled along the protruding member 210SA about the pivot axis AX1. For example, the center of the protruding member 210SA of the connecting rod 210 is spaced apart from the axis AX1 by a distance Y. When the surface 206SF of plate 206 is in substantial contact with the surface 205SF of frame 205, the center of elongated groove 220 (e.g., longitudinal axis 220X) may be spaced apart from axis AX1 by a distance Y'. Here, the amount of relative movement D is the difference between distance Y and distance Y', wherein, as connecting rod 210 pivots about axis AX1, protruding member 210SA intersects with elongated groove 220 so that plate 206 moves in direction 299A by an amount equal to distance D (thereby separating surfaces 206SF and 205SF by distance D).

[0018] In one aspect, the dispensing head 130 includes a dispensing head drive 110DH. The dispensing head drive 110DH includes a drive motor 110M and a shuttle 280. In one aspect, the drive motor 110M is mounted to a plate 206 via a motor mount, the motor mount including a bridge member 281 spanning a frame 205 and a mounting block 282 mounted to the bridge member 281, wherein the drive motor 110M is mounted to the mounting block 282. In other aspects, the drive motor 110M can be mounted to the plate 206 in any suitable manner. Here, the bridge member 281 is mounted to the plate 206 such that the drive motor 110M is fixed to the plate 206 for movement relative to the frame 205 in direction 299 upon actuation of the dispensing head drive 110DH. For example, in one scenario, the frame 205 is kept stationary by the transport device 120, while the plate 206 and the motor 110M move relative to the frame 205 in direction 299; and in another scenario, the plate 206 is kept stationary by the transport device 120, while the frame moves relative to the motor 110M and the plate 206.

[0019] One or more guide members 283 are coupled to mounting block 282 and extend longitudinally in direction 297. A shuttle 280 is movably coupled to one or more guide members 283, thereby moving along the guide members 283 in direction 297. In one aspect, the shuttle 280 includes one or more bushings 280B that allow sliding movement of the shuttle 280 along one or more guide members 283.

[0020] Still referencing Figure 2 and Figure 2A The drive motor 110M is driven to the shuttle 280 via a screw drive transmission including a screw 284 and a nut 285. In other aspects, the drive motor 110M can be driven to the shuttle 280 via any suitable transmission, including but not limited to belt drives, chain drives, and gear drives. In yet another aspect, the drive motor 110M can be a linear actuator, wherein one end of the linear actuator is connected to the bridge member 281, and the other end of the linear actuator is connected to the shuttle 280. Figure 2 As shown, screw 284 is connected to drive member 110MD (such as a drive shaft) of drive motor 110M in any suitable manner, such that drive member 110MD and screw 284 rotate uniformly about the axis of rotation DMX of drive member. Nut 285 is mounted to shuttle 280 in any suitable manner, such that screw 284 engages nut 285, wherein rotation of screw 284 moves shuttle bidirectionally in direction 297. For example, when drive motor 110M rotates screw 284 in a first direction, shuttle moves in direction 297A, and when drive motor 110M rotates screw in a second direction (opposite to the first direction), shuttle moves in direction 297B.

[0021] The shuttle 280 includes elongated slots 289, wherein the longitudinal axis 289X of each of the elongated slots 289 is arranged along the direction 299 of relative movement between the frame 205 and the plate 206. In one aspect, each of one or more connecting rods 210 includes a protruding member 210SB, which is substantially similar to a protruding member 210SA, wherein the protruding member 210SB includes a pin 210SBS extending laterally from the corresponding connecting rod 210 and a bushing 210SBB mating on the pin 210SBS; in other aspects, the protruding member may be a single component coupled to the corresponding connecting rod 210 in any suitable manner, or may be formed as a single construction with the corresponding connecting rod 210. The protruding member 210SB is positioned adjacent to the second end 210E2 of the corresponding connecting rod 210. The protruding member 210SB is dimensioned such that there is a clearance fit between the protruding member 210SB and the elongated slot 289, such that as the shuttle 280 moves in direction 297, the protruding members 210SB of one or more connecting rods 210 move along the longitudinal axis 289X within the elongated slot 289, and the intersection between the protruding member 210SB and the longitudinal sides 289L1, 289L2 of the elongated slot 289 causes the corresponding connecting rod 210 to pivot about axis AXL (e.g., axis AX1) to achieve relative movement between plate 206 and frame 205 in direction 299. As can be appreciated, the movement of the protruding member 210SB along the longitudinal axis 289X within the elongated slot 289 allows the drive motor 110M (and the screw 284, guide member 283, nut 285 and shuttle 280) and plate 206 to move relative to frame 205 in direction 299. When using a low-power actuator with a small size, the actuation of plate 206 or frame 205 in direction 299 relative to another of plate 206 or frame 205 utilizes one or more connecting rods 210 and screw drives (e.g., screw 284 and nut 285) to amplify the force provided by drive motor 110M (e.g., to provide up to several hundred pounds of force) for compressing / expanding one or more of the first adjustable sealing member 210A and the second adjustable sealing member 210B to connect pipette 399 to one or more pipette mandrels 200-200n.

[0022] As described above, the movement of shuttle 280 in direction 297A causes one or more connecting rods 210 to pivot about axis AX1 in direction 298A, causing one of plate 206 and frame 205 to move relative to the other in direction 299A. Similarly, the movement of shuttle 280 in direction 297B causes one or more connecting rods 210 to pivot about axis AX1 in direction 298B, causing one of plate 206 and frame 205 to move relative to the other in direction 299B. Thus, one of plate 206 and frame 205 reciprocates relative to the other, wherein the amount of reciprocating movement corresponds to a distance D. Any suitable sensor 291 is operatively connected to the shuttle 280, drive motor 110M, one or more connecting rods 210, plate 206, and / or frame 205 to send position signals to the controller 150 of the automated pipetting device 100 to determine, for example, the position of the shuttle 280, drive motor 110M, one or more connecting rods 210, and / or plate relative to, for example, frame 205, for enabling the gripping and release of the pipette tip and dispensing head 130, as described herein.

[0023] refer to Figure 2 , Figure 3 , Figure 4 , Figure 5A and Figure 5B The dispensing head includes one or more (e.g., at least one) pipette mandrels 200-200n disposed thereon. In one aspect, each pipette mandrel 200-200n includes an elongated pin body 300 having an insertion end 300E1, wherein the insertion end 300E1 dispenses fluid collected by a pipette tip 399, such that the insertion end 300E1 may also be referred to as a dispensing end. Each of the one or more pipette mandrels 200-200n is configured to mate with any suitable pipette tip 399 (e.g., such as a disposable pipette tip). See also... Figures 9 to 11CAs an example, pipette tip 399 includes a generally cylindrical pipette collar 399C (which may or may not be tapered or flared). Pipette collar 399C has an insertion end 399E1 configured to intersect and automatically engage with the pipette mandrels 200-200n of the automated pipetting device 100. Pipette tip 399 also includes a dispensing tip 399DT extending from pipette collar 399C opposite the insertion end. Pipette collar 399C and / or dispensing tip 399DT may have any suitable outer and / or inner contours to facilitate a predetermined flow of fluid through pipette tip 399. In one aspect, pipette tip 399 may include one or more reinforcing features, such as ribs 1000, extending radially outward and longitudinally along one or more of pipette collar 399C and dispensing tip 399DT. Suitable examples of dispensing nozzle configurations that can be incorporated into pipette tip 399 are available from Petaluma, California. get.

[0024] In one aspect, the insertion end 300E1 of the elongated pin body 300 is configured to be inserted into the insertion end 399E1 of the substantially cylindrical collar 399C of the pipette tip 399, for example, the elongated pin body 300 of the pipette spindle 200 is first inserted into the pipette tip 399 with the insertion end 300E1. In another aspect, the elongated pin body 300 is coupled to a frame 205, wherein a plate 206 is movable relative to the elongated pin body 300. In one aspect, the elongated pin bodies 300 of one or more pipette mandrels 200-200n extend through corresponding orifices in plate 206, wherein a second end of the elongated pin body 300 (opposite to the insertion end 300E1) is coupled to a first member 205A of frame 205 (wherein, the orifice 300B of the elongated pin body 300 can be coupled to any suitable pump PM for purging air / gas or forcing fluid into orifice 300B for drawing fluid into or from pipettes 399 coupled to the corresponding pipette mandrels 200-200n). In other respects, the elongated pin body 300 may be connected to the plate 206, for example, wherein the frame 205 is movable relative to the plate 206, and the plate 206 provides a connection between the hole 300B of the elongated pin body 300 and the pump PM for venting or squeezing air / gas into the hole 300B for drawing fluid into or dispensing fluid from the pipette 399 connected to the corresponding pipette mandrel 200-200n.

[0025] An adjustable seal pair is installed on each pipette mandrel. The adjustable seal pair includes a first adjustable seal 310A and a second adjustable seal 310B. The first adjustable seal 310A is disposed on the elongated pin body 300 near the insertion end 300E1, such that the first adjustable seal 310A seals the pipette 399, which engages with a corresponding one of one or more pipette mandrels 200-200n. The second adjustable seal 310B is disposed on the elongated pin body 300 away from the insertion end 300E1, such that the second adjustable seal 310B seals the pipette 399, which engages with a corresponding one of one or more pipette mandrels 200-200n. Thus, the first adjustable seal 310A and the second adjustable seal 310B are separated at different lengths S1 and S2 from the insertion end 300E1 of the elongated pin body 300.

[0026] In one aspect, it also refers to Figure 5C As the corresponding pipette mandrel 200-200n is inserted into the pipette 399, the first adjustable seal 310A forms a clearance fit CF2 with the pipette 399. In one aspect, the dispensing head 130 is a 96-pipette dispensing head having a clearance fit CF2 with a clearance ratio of approximately 1.02 to approximately 1 (e.g., the inner diameter 399D of the pipette 399 is approximately 1.02 times the outer diameter 310D2 of the first adjustable seal 310A). In another aspect, the dispensing head 130 is a 384-pipette dispensing head having a clearance fit CF2 with a clearance ratio of approximately 1.11 to approximately 1 (e.g., the inner diameter 399D of the pipette 399 is approximately 1.11 times the outer diameter 310D2 of the first adjustable seal 310A). In one aspect, the first adjustable seal 310A defines a buffer surface 400 for the corresponding pipette mandrel 200-200n, which buffers the insertion of the pipette mandrel 200-200n into the pipette 399 when the pipette 399 contacts the buffer surface 400 of the first adjustable seal 310A.

[0027] Also refer to Figure 9 , Figures 10A to 10C as well as Figures 11A to 11CHere, the pipette tip 399 includes a lower or first sealing surface 399S1 (also referred to as a sealing stop surface) that intersects with a first adjustable seal 310A. In one aspect, the lower sealing surface 399S1 has a circular surface, while in other aspects, the lower sealing surface 399S1 may have a flat or angled surface. The lower sealing surface is disposed on a surface (such as an inner surface) of the pipette tip collar 399C. The lower sealing surface 399S1 is configured to engage and cushion the insertion of the pipette tip spindle 200-200n, such that the cushioned contact between the lower sealing surfaces 399S1 causes the first adjustable seal 310A of the pipette tip spindle 200-200n to wedge against the lower sealing surface 399S1, achieving a substantially continuous circumferential contact seal by means of the radially tight contact between the lower sealing surface 399S1 and the pipette tip spindle 200-200n, as will be described in more detail below. The pipette tip 399 also includes an upper or second sealing surface 399S2 (also referred to as a sealing stop surface) that interacts with the second adjustable seal 310B. In one aspect, the upper sealing surface 399S2 may be a substantially flat sealing surface 399S2', such as... Figure 11B As best shown, in other respects, the upper sealing surface 399S2 may be a circular sealing surface 399S2' (e.g. Figure 10B (As best shown in the image), the combination of round and flat sealing surfaces 399S2 (such as...) Figure 9 (best shown in the image) or angled surfaces. The upper sealing surface is disposed on a surface (such as the inner surface) of the pipette collar 399C such that the upper sealing surface 399S2 is longitudinally spaced from the lower sealing surface 399S1. The upper sealing surface 399S2 is configured to mate with and engage a second adjustable seal 310B of the pipette spindle 200-200n, achieving a releasable grip and another substantially continuous circumferential contact seal between the upper sealing surface 399S2 and the pipette spindle 200-200n, as described in more detail below.

[0028] The buffer surface 400 of the first adjustable seal 310A is configured such that when the pipette spindle 200-200n is inserted into the pipette 399 in direction 299B, the buffer contact between the buffer surface 400 and the lower sealing surface 399S1 of the pipette 399 adjusts the first adjustable seal 310A (e.g., by deformation or displacement of the first adjustable seal 310A) to close the clearance fit CF2 between the pipette 399 and the first adjustable seal 310A, and wedges the first adjustable seal 310A against the pipette 399. A substantially continuous circumferential contact seal 410S1 is achieved by means of the radially tight contact between the pipette and the corresponding pipette spindle 200-200n.

[0029] In one respect, the second adjustable seal 310B is in the non-engaged position (see...) Figure 5A ) and engagement location (see Figure 5B Adjustable between the two. As the corresponding pipette spindle 200-200n is inserted into the pipette 399, the non-engaged position provides a clearance fit CF1 with the pipette 399 (which may be the same as or different from the clearance fit CF2). The engaged position causes the second adjustable seal 310B to contact the pipette 399, which engages with the corresponding pipette spindle 200-200n, achieving a releasable grip and another substantially continuous circumferential contact seal 410S2 (substantially similar to the substantially continuous circumferential contact seal 410S1) around the pipette 399 between the pipette 399 and the corresponding pipette spindle 200-200n. In one aspect, the dispensing head 130 is a 96-pipette dispensing head having a clearance fit CF1 with a clearance ratio of about 1.02 to about 1 (e.g., the inner diameter 399D of the pipette 399 is about 1.02 times the outer diameter 310D1 of the second adjustable seal 310B, wherein the outer diameter 310D1 may be the same as or different from the outer diameter 310D2 of the first adjustable seal 310A, for example, when the inner surface of the pipette 399 is tapered). In another aspect, the dispensing head 130 is a 384-pipette dispensing head having a clearance fit CF1 with a clearance ratio of about 1.11 to about 1 (e.g., the inner diameter 399D of the pipette 399 is about 1.11 times the outer diameter 310D1 of the second adjustable seal 310B).

[0030] Still referencing Figure 2 , Figure 3 , Figure 4 , Figure 5A and Figure 5BThe dispensing head 130 includes one or more (e.g., at least one) sealing adjustment members 450, such that each sealing adjustment member 450 forms a sleeve around a corresponding elongated pin body 300 of the pipette mandrel 200-200n, thereby being movably coupled to the corresponding elongated pin body 300 for movement relative to the corresponding elongated pin body 300 in direction 299. In one aspect, one or more sealing adjustment members 450 may be coupled to a plate 206 such that the plate 206 is common to one or more sealing adjustment members 450, and such that one or more sealing adjustment members 450 are bolted (e.g., bolts 284 and nuts 285, which are common to each of the sealing adjustment members 450) and operably connected via one or more connecting rods 210 to a drive motor 110M of the dispensing head drive 110DH (of the drive section 110). Here, the movement of plate 206 relative to frame 205 in direction 299B generated by drive motor 110M causes a corresponding movement in direction 299B of one or more sealing adjustment members 450 (e.g., one or more sealing adjustment members 450 and plate 206 move in unison) (see...). Figure 5A and 5B This causes one or more sealing adjustment members 450 to contact a corresponding one of the second adjustable seals 310B, thereby compressing / expanding the second adjustable seal 310B and achieving adjustment of the second adjustable seal 310B to an engaged position (e.g., closing the clearance fit CF1 to form a substantially continuous circumferential contact seal 410S2, and gripping the pipette 399 for retention on the corresponding pipette mandrel 200-200n). Similarly, movement of the plate 206 in direction 299A generated by the drive motor 110M causes corresponding movement of one or more sealing adjustment members 450 in direction 299A (see...). Figure 5A and 5B This allows one or more sealing adjustment members 450 to disengage from a corresponding one of the second adjustable seals 310B, thereby releasing the compression / contraction of the second adjustable seal 310B and enabling adjustment of the second adjustable seal 310B to a non-engaged position (e.g., opening the clearance fit CF to release the substantially continuous circumferential contact seal 410S2 and release the pipette 399 for removal from the corresponding pipette mandrel 200-200n). In one aspect, the first adjustable seal 310A and the second adjustable seal 310B may be O-rings having substantially the same hardness, while in other aspects, the hardness of the first adjustable seal 310A and the hardness of the second adjustable seal 310B may be different.

[0031] On the other hand, one or more sealing adjustment members 450 may be coupled to frame 205 such that frame 205 is common to one or more sealing adjustment members 450, and such that one or more sealing adjustment members 450 are bolted (e.g., bolts 284 and nuts 285, which are common to each of the sealing adjustment members 450) and operably connected via one or more connecting rods 210 to drive motor 110M of distribution head drive 110DH (of drive section 110). Here, the movement of frame 205 relative to plate 206 in direction 299B generated by drive motor 110M causes a corresponding movement of one or more sealing adjustment members 450 (e.g., one or more sealing adjustment members 450 and frame 205 move in unison) in direction 299B (see...). Figure 5A and 5B This causes one or more sealing adjustment members 450 to contact a corresponding one of the second adjustable seals 310B, thereby compressing / expanding the second adjustable seal 310B and achieving adjustment of the second adjustable seal 310B to an engaged position (e.g., closing the clearance fit CF1 to form a substantially continuous circumferential contact seal 410S2, and gripping the pipette 399 for retention on the corresponding pipette mandrel 200-200n). Similarly, movement of the frame 205 in direction 299A generated by the drive motor 110M causes corresponding movement of one or more sealing adjustment members 450 in direction 299A (see...). Figure 5A and 5B This causes one or more sealing adjustment members 450 to disengage from a corresponding one of the second adjustable seals 310B, thereby releasing the compression / contraction of the second adjustable seal 310B and achieving adjustment of the second adjustable seal 310B to a non-engaged position (e.g., opening the clearance fit CF to release the substantially continuous circumferential contact seal 410S2 and release the pipette 399 for removal from the corresponding pipette spindle 200-200n).

[0032] refer to Figure 2 , Figure 2A , Figure 4 , Figure 5A , Figure 5B and Figure 7 The exemplary operation of the automated pipetting device 100 will be described below. As described above, a dispensing head 130 is provided ( Figure 7 (frame 700), and the dispensing head 130 includes one or more pipette mandrels 200-200n, each having an elongated pin body 300 having an insertion end 300E1. A first adjustable seal 310A is provided. Figure 7 , frame 710), and provides a second adjustable seal 310B ( Figure 7 (frame 720). One or more pipette mandrels 200-200n are inserted into corresponding pipettes 399, wherein the insertion of one or more pipette mandrels 200-200n is buffered when they contact the buffer surface 400 of the first adjustable seal 310A. Figure 7 (frame 730), wherein, as described above, the buffer contact adjusts the first adjustable seal 310A, and wedging the first adjustable seal 310A against the pipette tip 399, thereby achieving a substantially continuous circumferential contact seal 410S1. In one aspect, via the transport device 120 ( Figure 1 The transport device 120 moves the dispensing head 130 in direction 299B to align one or more pipette mandrels 200-200n with the corresponding pipette tip 399. Alternatively, the dispensing head 130 moves in direction 299B to insert one or more pipette mandrels 200-200n into the corresponding pipette tip 399. In another scenario, the pipette tips 399 are inserted into the corresponding pipette tips 399 by movement of the pipette tips 399, for example, where the pipette tips 399 are arranged in a tray, and the tray is moved by any suitable transport device (which may be substantially similar to transport device 120). The movement of the pipette tip 399 can align the pipette tip 399 with one or more pipette mandrels 200-200n, wherein one of the pipette tip 399 and the dispensing head 130 moves in direction 299B relative to the other of the pipette tip 399 and the dispensing head 130 to insert one or more pipette mandrels 200-200n into the corresponding pipette tip 399.

[0033] The second adjustable seal 310B is adjusted between a non-engaged position and an engaged position. The non-engaged position provides a clearance fit CF1 between the respective pipette mandrel 200-200n and the pipette tip 399. In the engaged position, the second adjustable seal 310B contacts the pipette tip 399, which engages with the respective pipette mandrel 200-200n, achieving a releasable grip and another substantially continuous circumferential contact seal 410S2 around the pipette tip between the pipette tip 399 and the respective pipette mandrel 200-200n. Here, the substantially continuous circumferential contact seal 410S2 between the pipette tip 399 and the second adjustable seal 310B is achieved using only one actuating motion, for example, using the actuating motion of the dispensing head drive 110DH, which is substantially common to each of the one or more pipette mandrels 200-200n. For example, each of one or more sealing adjustment members 450 is operatively coupled to a common drive member (e.g., such as plate 206, screw 284, shuttle 280, and / or connecting rod 210 of the distribution head drive 110DH) with another sealing adjustment member 450, thereby actuating each of one or more sealing adjustment members 450 and the other sealing adjustment member 450 for movement in direction 299 in conjunction with the common actuation of the common drive member (e.g., such as movement of plate 206 in direction 299, screw 284 for driving rotation of shuttle 280, movement of shuttle 280 in direction 297, and / or pivoting of connecting rod 210 in direction 298).

[0034] The pipette tip 399 is released from the corresponding pipette spindle 200-200n in a manner substantially opposite to that described above.

[0035] For reference Figure 2 , Figure 2A , Figure 6A and Figure 6BIn one aspect, one or more sealing member adjustment members 450A (unless otherwise specified, which may be substantially similar to the sealing adjustment member 450 described above) include a first portion 450A1 and a second portion 450A2, such that each of the one or more sealing member adjustment members 450A commonly engages a first adjustable seal 310A and a second adjustable seal 310B in the sealing pair. For example, as described above, the first adjustable seal 310A and the second adjustable seal 310B are disposed on respective pipette mandrels 200-200n, such that the first adjustable seal 310A and the second adjustable seal 310B are spaced apart from each other. One or more seals are movably coupled to a second portion 450A2 of the adjusting member 450A, which is connected to a corresponding elongated pin body 300 of the respective pipette mandrel 200-200n, thereby forming a sleeve in a manner substantially similar to that described above with respect to the sealing adjusting member 450, the sleeve being movable in direction 299 relative to the elongated pin body 300. The second portion 450A2 is disposed between the first adjustable seal 310A and the second adjustable seal 310B, near the insertion end 300E1 of the elongated pin body 300. One or more seals are also movably coupled to a first portion 450A1 of the adjusting member 450A, which is connected to a corresponding elongated pin body 300 of the respective pipette mandrel 200-200n, thereby forming a sleeve in a manner substantially similar to that described above with respect to the sealing adjusting member 450, the sleeve being movable in direction 299 relative to the elongated pin body 300. The first part 450A1 is positioned away from the insertion end 300E1 of the elongated pin body 300, and is opposite to the second part 450A2 relative to the second adjustable seal 210B.

[0036] In one aspect, the first portion 450A1 and the second portion 450A2 are separated from each other and distinct, such that each of the first portion 450A1 and the second portion 450A2 is independently movable along the elongated pin body 300. In another aspect, one of the first portion 450A1 and the second portion 450A2 is slidably engaged with the other of the first portion 450A1 and the second portion 450A2, such that the first portion 450A1 and the second portion 450A2 are connected to each other while still being movable relative to each other.

[0037] In a manner similar to that described above, one or more pipette mandrels 200-200n are inserted into corresponding pipette tips 399, such that a first adjustable seal 310A cushions the insertion of one or more pipette mandrels 200-200n into the corresponding pipette tips 399. As described above, an actuable plate 206 (or frame 205) is movable in direction 299B, causing a first portion 450A1 of the seal against the adjusting member 450A to move in direction 299B to contact a second sealing member 310B, which is coupled to the plate 206 (or frame 205) as described above. The contact between the first portion 450A1 and the second sealing member 310B results in a corresponding contact between the second portion 450A2 and the first sealing member 310A (e.g., due to the second sealing member 310B pushing the second portion 450A2 in direction 299B). Thus, the seals on the adjusting member 450A cause each of the first adjustable seal 310A and the second adjustable seal 310B to move from its respective non-engaged position (e.g., Figure 6A As shown in the image, the parts are adjusted to the engagement position almost simultaneously. Figure 6B (as shown in the image). In the non-engaged position, each of the first adjustable seal 310A and the second adjustable seal 310B forms a corresponding clearance fit CF1, CF2 between the corresponding pipette spindle 200-200n and the corresponding pipette tip 399. In the engaged position (in... Figure 6B As shown in the image, each of the first adjustable seal 310A and the second adjustable seal 310B contacts the corresponding pipette tip 399, which is fitted to the corresponding pipette spindle 200-200n, achieving a releasable grip. The first adjustable seal 310A and the second adjustable seal 310B, which are aligned in the seal, grip the corresponding pipette tip 399 substantially simultaneously, and the pipette tip 399 is fitted to the corresponding pipette spindle 200-200n.

[0038] As described above, the first adjustable seal 310A and the second adjustable seal 310B may be O-rings with the same or different hardness, such that one or more of the substantially continuous circumferential contact seals 410S1, 410S2 (formed when the respective adjustable seals 310A, 310B are adjusted to the engaged position) are established between each of the first adjustable seals 310A and the second adjustable seal 310B and the pipette tip 399 without excessive expansion / deformation of one or more of the first adjustable seals 310A and the second adjustable seal 310B. The first adjustable seals 310A and the second adjustable seal 310B may also be configured to provide a predetermined alignment between each of the pipette spindles 200-200n and the respective pipette tip 399.

[0039] For reference Figure 2 , Figure 2A , Figure 6A , Figure 6B and Figure 8 The exemplary operation of the automated pipetting device 100 will be described (unless otherwise stated, it may be substantially similar to that described above). In one aspect, frame 205 is provided ( Figure 8 (frame 800). In one aspect, frame 205 is part of frame 101. A drive section 110 is provided, and said drive section 110 is mounted to the frame (frame 800). Figure 8 (frame 810). A distribution head 130 is provided, and the distribution head 130 is connected to the frame ( Figure 8 (frame 820). A pair of seals with adjustable seals (e.g., including a first adjustable seal 310A and a second adjustable seal 310B) is provided, and the pair of seals is mounted to each pipette mandrel of the dispensing head 130. Figure 8 (frame 830). A seal pair adjustment member 450A is provided, and the seal pair adjustment member 450A is coupled to each pipette spindle 200-200n and operably connected to a drive section 110, such as a dispensing head drive 110DH, such that actuation of the drive section 110 achieves relative movement between the seal pair adjustment member 450A and each pipette spindle 200-200n coupled to the seal pair adjustment member 450A. Figure 8 (840).

[0040] In one respect, via transport device 120 ( Figure 1 The movement of the dispensing head 130 involves inserting one or more pipette mandrels 200-200n into the corresponding pipette tip 399. The transport device 120 aligns one or more pipette mandrels 200-200n with the corresponding pipette tip 399, and then moves the dispensing head 130 in direction 299B to insert one or more pipette mandrels 200-200n into the corresponding pipette tip 399. Both the first adjustable seal 310A and the second adjustable seal 310B in the seal pair engage with the seal pair adjustment member 450A so that each of the first adjustable seal 310A and the second adjustable seal 310B in the seal pair moves from the corresponding non-engaged position (see...). Figure 6A They are adjusted to the engagement position almost simultaneously (see...) Figure 6B (as described above).

[0041] Releasable gripping is achieved in a manner substantially similar to that described above, through substantially continuous circumferential contact seals 410S1, 410S2, which are formed by contact between the first adjustable seal 310A and the second adjustable seal 310B and the pipette 399. Moreover, in a manner substantially similar to that described above, the substantially continuous circumferential contact seals 410S1, 410S2 between the first adjustable seal 310A and the second adjustable seal 310B of the pipette tip 399 and the resulting releasable grip, and the first adjustable seal 310A and the second adjustable seal 310B of the other pipette tip 200-200n (which grips the other corresponding pipette tip 399), are achieved substantially uniformly by a single actuating motion, for example, by an actuating motion of the dispensing head drive 110DH, which is substantially common to each of the one or more pipette tip 200-200n. For example, each of one or more sealing adjustment members 450A is operatively coupled to a common drive member (e.g., such as plate 206, screw 284, shuttle 280, and / or connecting rod 210 of the distribution head drive 110DH) with another sealing adjustment member 450, thereby actuating each of one or more sealing adjustment members 450 and the other sealing adjustment member 450 for movement in direction 299 in conjunction with the common actuation of the common drive member (e.g., such as movement of plate 206 in direction 299, screw 284 for driving rotation of shuttle 280, movement of shuttle 280 in direction 297, and / or pivoting of connecting rod 210 in direction 298).

[0042] The pipette tip 399 is released from the corresponding pipette spindle 200-200n in a manner substantially opposite to that described above.

[0043] According to one or more aspects of the disclosed embodiments, the automated pipetting device includes: A dispensing head having at least one pipette mandrel, the pipette mandrel having an elongated pin body having an insertion end, the at least one pipette mandrel being configured to mate with a pipette, the elongated pin body of the at least one pipette mandrel being first inserted into the pipette with the insertion end; A first adjustable seal is disposed on the elongated pin body and configured such that the first adjustable seal seals the pipette, the pipette engaging with the at least one pipette mandrel, the first adjustable seal being positioned close to the insertion end; A second adjustable seal is disposed on the elongated pin body and configured such that the second adjustable seal seals the pipette, which engages with the at least one pipette mandrel, and the second adjustable seal is positioned remote from the insertion end; and The first adjustable seal defines a buffer surface for the at least one pipette mandrel. When in contact with the pipette and the buffer surface of the first adjustable seal, the buffer surface buffers the insertion of the at least one pipette mandrel into the pipette. The buffer surface is configured such that the buffer contact adjusts the first adjustable seal and wedges the first adjustable seal against the pipette. A substantially continuous circumferential contact seal is achieved by utilizing the radially tight contact between the pipette and the at least one pipette mandrel. The second adjustable seal is adjustable between a non-engaged position and an engaged position, wherein the non-engaged position provides a clearance fit between the at least one pipette mandrel and the pipette, and in the engaged position, the second adjustable seal contacts the pipette, which engages with the at least one pipette mandrel, achieving a releasable grip and another substantially continuous circumferential contact seal around the pipette between the pipette and the at least one pipette mandrel.

[0044] According to one or more aspects of the disclosed embodiments, as the at least one pipette mandrel is inserted, the first adjustable seal forms a clearance fit with the pipette.

[0045] According to one or more aspects of the disclosed embodiments, the buffer surface of the first adjustable seal makes buffer contact with the pipette and the first adjustable seal is adjusted to close with a clearance fit between the first adjustable seal and the pipette.

[0046] According to one or more aspects of the disclosed embodiments, as the at least one pipette mandrel is inserted, both the first adjustable seal and the second adjustable seal form a clearance fit with the pipette.

[0047] According to one or more aspects of the disclosed embodiments, the dispensing head is a 96 pipette dispensing head having a clearance fit with a clearance ratio of 1.02 to 1.

[0048] According to one or more aspects of the disclosed embodiments, the dispensing head is a 384 pipette dispensing head having a clearance fit with a clearance ratio of 1.11 to 1.

[0049] According to one or more aspects of the disclosed embodiments, the automated pipetting device further includes: a drive section connected to the dispensing head; and at least one sealing adjustment member operatively connected to the drive section and coupled to the at least one pipetting tip spindle, wherein the at least one sealing adjustment member engages with the second adjustable seal and achieves adjustment between the non-engaged position and the engaged position.

[0050] According to one or more aspects of the disclosed embodiments, in the engagement position, the contact between the pipette tip and the second adjustable seal forms a substantially continuous circumferential contact seal between the at least one pipette mandrel and the pipette tip, wherein the substantially continuous circumferential contact seal is achieved by only one actuating motion, which is substantially common for each of the at least one pipette mandrel.

[0051] According to one or more aspects of the disclosed embodiments, the at least one sealing adjustment member coupled to the at least one pipette mandrel is operatively coupled to a common drive member with another sealing adjustment member, such that each of the at least one sealing adjustment member and the other sealing adjustment member are actuated in unison by means of the common actuation action of the common drive member.

[0052] According to one or more aspects of the disclosed embodiments, the drive section includes a motor mounted to the frame of the automated pipetting device and operably connected via a common screw to each sealing adjustment member, the sealing adjustment member being coupled to the at least one pipetting tip spindle.

[0053] According to one or more aspects of the disclosed embodiments, the motor is operably connected to each sealing adjustment member via connecting rods that collectively actuate each sealing adjustment member, the sealing adjustment member being coupled to the at least one pipette mandrel such that the second adjustable seal grips the pipette substantially in agreement with another second adjustable seal gripping another corresponding pipette, the pipette engaging with each of the at least one pipette mandrel.

[0054] According to one or more aspects of the disclosed embodiments, the automated pipetting device includes: frame; The drive section is mounted to the frame; A dispensing head, connected to the frame, has a plurality of pipette mandrels, each of the plurality of pipette mandrels having an elongated pin body having a dispensing end, each of the plurality of pipette mandrels being configured to mate with a corresponding pipette, the elongated pin body of the pipette mandrel being first inserted into the pipette with the dispensing end; An adjustable seal pair is mounted to each pipette mandrel, wherein one seal in the adjustable seal pair is separated from the other seal in the seal pair at different lengths from the dispensing end of the elongated pin body; and A pair of adjusting members, connected to each pipette spindle and operably connected to the drive section, such that actuation of the drive section enables relative movement between the adjusting members and each pipette spindle connected to the adjusting members. The adjusting members collectively engage two seals in the pair, adjusting each seal from a corresponding non-engaged position to an engaged position substantially simultaneously. In the non-engaged position, each seal forms a clearance fit between the corresponding pipette spindle and the corresponding pipette. In the engaged position, each seal contacts the corresponding pipette, which engages with the corresponding pipette spindle. The two seals in the pair enable a releasable gripping of the corresponding pipette engaged with the corresponding pipette spindle, achieving substantially simultaneous gripping.

[0055] According to one or more aspects of the disclosed embodiments, the substantially simultaneous grasping of a corresponding pipette that is mated to a corresponding pipette mandrel by means of two seals in the seal pair of the adjustable seal is achieved by a single actuation movement, which is substantially common to each of the plurality of pipette mandrels mated to the corresponding pipette.

[0056] According to one or more aspects of the disclosed embodiments, in the engagement position, a substantially continuous circumferential contact seal is formed between the contact between the respective pipette tip and one of the seals in the pair of seals of the adjustable seal, and between the respective pipette spindle and the respective pipette tip mating thereto.

[0057] According to one or more aspects of the disclosed embodiments, when the one sealing element is adjusted to the engagement position, a substantially continuous circumferential contact seal is achieved.

[0058] According to one or more aspects of the disclosed embodiments, in the engagement position, the contact between the two seals in the seal pair of the respective pipette and the adjustable seal forms more than one substantially continuous circumferential contact seal between the respective pipette mandrel and the respective pipette mating thereto.

[0059] According to one or more aspects of the disclosed embodiments, when each seal in the pair of seals of the adjustable seal is adjusted to the engagement position, more than one substantially continuous circumferential contact seal is achieved.

[0060] According to one or more aspects of the disclosed embodiments, more than one substantially continuous circumferential contact seal is achieved substantially simultaneously using only one actuating motion, which is substantially common to each of the plurality of pipette mandrels.

[0061] According to one or more aspects of the disclosed embodiments, each seal pair adjustment member coupled to the plurality of pipette mandrels is operatively coupled to a drive member common to another seal pair adjustment member, such that each of the seal pair adjustment members and the other seal pair adjustment member are actuated in unison by the common actuation action of the common drive member.

[0062] According to one or more aspects of the disclosed embodiments, the drive section includes a motor mounted to the frame and operably connected via a common screw to each seal pair adjustment member, the seal pair adjustment member being coupled to the plurality of pipette mandrels.

[0063] According to one or more aspects of the disclosed embodiments, the motor is operably connected to each seal pair adjustment member via connecting rods, the connecting rods collectively actuating each seal pair adjustment member, the seal pair adjustment members being coupled to the plurality of pipette mandrels such that each seal pair of adjustable seals grips the corresponding pipette substantially in agreement with another seal pair of adjustable seals gripping another corresponding pipette, the pipettes engaging with each of the plurality of pipette mandrels.

[0064] According to one or more aspects of the disclosed embodiments, one of the adjustable seals in the pair of seals defines a buffer surface of a corresponding pipette mandrel, the buffer surface buffering the insertion of the corresponding pipette mandrel into the corresponding pipette.

[0065] According to one or more aspects of the disclosed embodiments, the dispensing head is a 96 pipette dispensing head having a clearance fit with a clearance ratio of 1.02 to 1.

[0066] According to one or more aspects of the disclosed embodiments, the dispensing head is a 384 pipette dispensing head having a clearance fit with a clearance ratio of 1.11 to 1.

[0067] According to one or more aspects of the disclosed embodiments, the automated pipetting method includes: A dispensing head is provided having at least one pipette mandrel, the pipette mandrel having an elongated pin body having an insertion end, the at least one pipette mandrel being configured to mate with a pipette, the elongated pin body of the at least one pipette mandrel being first inserted into the pipette with the insertion end; A first adjustable seal is provided on the elongated pin body and configured such that the first adjustable seal seals the pipette, the pipette engaging with the at least one pipette mandrel, the first adjustable seal being positioned close to the insertion end; A second adjustable seal is provided, which is disposed on the elongated pin body and configured such that the second adjustable seal seals the pipette, which engages with the at least one pipette mandrel, and the second adjustable seal is positioned away from the insertion end; By utilizing the buffer surface of the at least one pipette mandrel defined by the first adjustable seal, when the pipette and the buffer surface of the at least one pipette mandrel come into contact, insertion of the at least one pipette mandrel into the pipette is buffered, wherein the buffer contact of the buffer surface adjusts the first adjustable seal and causes the first adjustable seal to wedge against the pipette, achieving a substantially continuous circumferential contact seal by means of the radially tight contact between the pipette and the at least one pipette mandrel; and The second adjustable seal is adjusted between a non-engaged position and an engaged position, wherein the non-engaged position provides a clearance fit between the at least one pipette mandrel and the pipette, and in the engaged position, the second adjustable seal contacts the pipette, which engages with the at least one pipette mandrel, achieving a releasable grip and another substantially continuous circumferential contact seal around the pipette between the pipette and the at least one pipette mandrel.

[0068] According to one or more aspects of the disclosed embodiments, the automated pipetting method further includes, as the at least one pipette mandrel is inserted, forming a clearance fit with the pipette using the first adjustable seal.

[0069] According to one or more aspects of the disclosed embodiments, the buffer surface of the first adjustable seal makes buffer contact with the pipette and the first adjustable seal is adjusted to close with a clearance fit between the first adjustable seal and the pipette.

[0070] According to one or more aspects of the disclosed embodiments, the automated pipetting method further includes, as the at least one pipette mandrel is inserted, forming a clearance fit with the pipette using both the first adjustable seal and the second adjustable seal.

[0071] According to one or more aspects of the disclosed embodiments, the dispensing head is a 96 pipette dispensing head having a clearance fit with a clearance ratio of 1.02 to 1.

[0072] According to one or more aspects of the disclosed embodiments, the dispensing head is a 384 pipette dispensing head having a clearance fit with a clearance ratio of 1.11 to 1.

[0073] According to one or more aspects of the disclosed embodiments, the automated pipetting method further includes providing: a drive section connected to the dispensing head; and at least one sealing adjustment member operatively connected to the drive section and coupled to the at least one pipetting tip spindle, wherein the at least one sealing adjustment member engages with the second adjustable seal and realizes adjustment between the non-engaged position and the engaged position.

[0074] According to one or more aspects of the disclosed embodiments, the automated pipetting method further includes forming a substantially continuous circumferential contact seal between the at least one pipette mandrel and the pipette by utilizing the contact between the pipette tip and the second adjustable seal in the engaged position, wherein the substantially continuous circumferential contact seal is achieved by only one actuating motion, the actuating motion being substantially common for each of the plurality of pipette mandrels.

[0075] According to one or more aspects of the disclosed embodiments, the at least one sealing adjustment member coupled to the at least one pipette mandrel is operatively coupled to a common drive member with another sealing adjustment member, such that each of the at least one sealing adjustment member and the other sealing adjustment member are actuated in unison by means of the common actuation action of the common drive member.

[0076] According to one or more aspects of the disclosed embodiments, the automated pipetting method further includes providing a motor for the drive section, the motor being mounted to the frame of the automated pipetting device and operably connected via a common screw to each sealing adjustment member, the sealing adjustment member being coupled to the at least one pipetting tip mandrel.

[0077] According to one or more aspects of the disclosed embodiments, the automated pipetting method further includes operably connecting the motor to each sealing adjustment member via connecting rods, the connecting rods collectively actuating each sealing adjustment member, the sealing adjustment member being coupled to the at least one pipette mandrel such that the second adjustable seal grips the pipette substantially in agreement with another second adjustable seal gripping another corresponding pipette, the pipette engaging with each of the at least one pipette mandrels.

[0078] According to one or more aspects of the disclosed embodiments, the automated pipetting method includes: Provide a framework; A drive section is provided, which is mounted to the frame; A dispensing head is provided, which is connected to the frame and has a plurality of pipette mandrels, each of the plurality of pipette mandrels having an elongated pin body having a dispensing end, each of the plurality of pipette mandrels being configured to mate with a corresponding pipette, the elongated pin body of the pipette mandrel being first inserted into the pipette with the dispensing end; A pair of adjustable seals is provided, the pair of seals being mounted to each pipette mandrel, wherein one seal in the pair of adjustable seals is separated from the other seal in the pair of adjustable seals at different lengths from the dispensing end of the elongated pin body. A seal pair adjustment member is provided, the seal pair adjustment member being coupled to each pipette spindle and operably connected to the drive section, such that actuation of the drive section achieves relative movement between the seal pair adjustment member and each pipette spindle coupled to the seal pair adjustment member; and The two seals of the adjustable seal pair engage with the adjusting member of the seal pair, such that each seal of the adjustable seal pair is adjusted substantially simultaneously from a corresponding non-engaged position to an engaged position. In the non-engaged position, each seal of the adjustable seal pair forms a clearance fit between a corresponding pipette spindle and a corresponding pipette. In the engaged position, each seal of the adjustable seal pair contacts a corresponding pipette, which engages with a corresponding pipette spindle. The two seals of the adjustable seal pair enable a releasable gripping of the corresponding pipette engaged with the corresponding pipette spindle substantially simultaneously.

[0079] According to one or more aspects of the disclosed embodiments, the substantially simultaneous grasping of a corresponding pipette that is mated to a corresponding pipette mandrel by means of two seals in the seal pair of the adjustable seal is achieved by a single actuation movement, which is substantially common to each of the plurality of pipette mandrels mated to the corresponding pipette.

[0080] According to one or more aspects of the disclosed embodiments, the automated pipetting method further includes forming a substantially continuous circumferential contact seal between the respective pipetting mandrel and the respective pipetting tip mated thereto by utilizing contact between the respective pipetting tip and one of the seals in the mating position of the respective pipetting tip and the seal of the adjustable seal.

[0081] According to one or more aspects of the disclosed embodiments, the automated pipetting method further includes achieving a substantially continuous circumferential contact seal when the one seal is adjusted to the engagement position.

[0082] According to one or more aspects of the disclosed embodiments, the automated pipetting method further includes forming more than one substantially continuous circumferential contact seal between a respective pipette mandrel and a respective pipette mating thereto by utilizing the contact between two seals in the respective pipette tip and the seal pair at the engagement position.

[0083] According to one or more aspects of the disclosed embodiments, the automated pipetting method further includes achieving more than one substantially continuous circumferential contact seal when each seal in the pair of adjustable seals is adjusted to the engagement position.

[0084] According to one or more aspects of the disclosed embodiments, the automated pipetting method further includes more than one substantially continuous circumferential contact seal achieved substantially simultaneously by a single actuating motion, the actuating motion being substantially common for each of the plurality of pipetting mandrels.

[0085] According to one or more aspects of the disclosed embodiments, each seal pair adjustment member coupled to the plurality of pipette mandrels is operatively coupled to a drive member common to another seal pair adjustment member, such that each of the seal pair adjustment members and the other seal pair adjustment member are actuated in unison by the common actuation action of the common drive member.

[0086] According to one or more aspects of the disclosed embodiments, the automated pipetting method further includes providing a motor for the drive section, the motor being mounted to the frame and operably connected via a common screw to each seal pair adjustment member, the seal pair adjustment member being coupled to the plurality of pipetting tip mandrels.

[0087] According to one or more aspects of the disclosed embodiments, the automated pipetting method further includes operably connecting the motor to each pair of seal adjustment members via connecting rods, the connecting rods collectively actuating each pair of seal adjustment members, the pair of seal adjustment members being coupled to the plurality of pipette mandrels such that each pair of seals of the adjustable seals grips the corresponding pipette substantially in agreement with the seal pair of another adjustable seal that grips another corresponding pipette, the pipettes engaging with each of the plurality of pipette mandrels.

[0088] According to one or more aspects of the disclosed embodiments, the automated pipetting method further includes cushioning the insertion of the respective pipetting mandrel into the respective pipetting nozzle using a cushioning surface defined by an adjustable seal of one of the pair of adjustable seals of the respective pipetting mandrel.

[0089] According to one or more aspects of the disclosed embodiments, the dispensing head is a 96 pipette dispensing head having a clearance fit with a clearance ratio of 1.02 to 1.

[0090] According to one or more aspects of the disclosed embodiments, the dispensing head is a 384 pipette dispensing head having a clearance fit with a clearance ratio of 1.11 to 1.

[0091] According to one or more aspects of the disclosed embodiments, a pipette tip for an automated pipetting device is provided. The pipette tip includes: A pipette collar, having an insertion end, is configured to intersect and automatically engage with the pipette mandrel of the automated pipetting device; A dispensing nozzle extends from the pipette collar and is opposite the insertion end; A first sealing stop surface is disposed on the surface of the pipette collar. The first sealing stop surface is configured to engage and cushion the insertion of the pipette spindle, such that the cushioned contact between the first sealing stop surfaces causes a first sealing portion of the pipette spindle to wedge against the first sealing stop surface. A substantially continuous circumferential contact seal is achieved through the radially tight contact between the first sealing stop surface and the pipette spindle. A second sealing stop surface is disposed on the surface of the pipette collar such that the second sealing stop surface is longitudinally spaced from the first sealing stop surface. The second sealing stop surface is configured to engage and cooperate with a second sealing portion of the pipette spindle, thereby achieving a releasable grip and another substantially continuous circumferential contact seal between the second sealing stop surface and the pipette spindle.

[0092] According to one or more aspects of the disclosed embodiments, as the pipette mandrel is inserted into the pipette mandrel collar, the first sealing stop surface forms a clearance fit with the pipette mandrel.

[0093] According to one or more aspects of the disclosed embodiments, as the pipette mandrel is inserted into the pipette mandrel collar, both the first sealing stop surface and the second sealing stop surface form a clearance fit with the pipette mandrel.

[0094] According to one or more aspects of the disclosed embodiments, an automated pipetting apparatus is provided. The automated pipetting apparatus includes: Distribution header, including At least one pipette mandrel has an elongated pin body with a mandrel insertion end. A first adjustable seal is disposed on the elongated pin body near the mandrel insertion end, and A second adjustable seal is provided on the elongated pin body away from the mandrel insertion end; At least one pipette tip, including A pipette collar, having a nozzle insertion end, is configured to mate with the at least one pipette mandrel, wherein the elongated pin body is first inserted into the nozzle insertion end of the pipette collar with the mandrel insertion end. A first sealing stop surface is disposed on the surface of the pipette collar, the first sealing stop surface being configured to engage the first adjustable seal such that the first adjustable seal seals the pipette, the pipette fitting into the at least one pipette spindle, and A second sealing stop surface is disposed on the surface of the pipette collar, such that the second sealing stop surface is longitudinally spaced from the first sealing stop surface. The second sealing stop surface is configured to engage a second adjustable seal, such that the second adjustable seal seals the pipette, the pipette fitting into the at least one pipette spindle; and Wherein, the first adjustable seal defines a buffer surface of the at least one pipette mandrel, which buffers insertion of the at least one pipette mandrel into the pipette collar when in contact with the first sealing stop surface and the buffer surface of the first adjustable seal. The buffer surface is configured such that the buffer contact adjusts the first adjustable seal and wedges the first adjustable seal against the first sealing stop surface. A substantially continuous circumferential contact seal is achieved through the radially tight contact between the first sealing stop surface and the at least one pipette mandrel. The second adjustable seal is adjustable between a non-engaged position and an engaged position. The non-engaged position provides a clearance fit between the at least one pipette mandrel and the pipette collar. In the engaged position, the second adjustable seal contacts the pipette collar, which engages with the at least one pipette mandrel, enabling a releasable grip and another substantially continuous circumferential contact seal around the pipette collar between the pipette collar and the at least one pipette mandrel.

[0095] According to one or more aspects of the disclosed embodiments, the dispensing head is a 96 pipette dispensing head having a clearance fit with a clearance ratio of 1.02 to 1.

[0096] According to one or more aspects of the disclosed embodiments, the dispensing head is a 384 pipette dispensing head having a clearance fit with a clearance ratio of 1.11 to 1.

[0097] It should be understood that the foregoing description is illustrative only regarding aspects of the disclosed embodiments. Various alternatives and modifications can be conceived by those skilled in the art without departing from the aspects of the disclosed embodiments.

Claims

1. An automated pipetting device, comprising: frame; The drive section is mounted to the frame; A dispensing head, connected to the frame, has a plurality of pipette mandrels, each of the plurality of pipette mandrels having an elongated pin body having a dispensing end, each of the plurality of pipette mandrels being configured to mate with a corresponding pipette, the elongated pin body of the pipette mandrel being received in the corresponding pipette; An adjustable seal pair is installed on each pipette mandrel, wherein one seal in the adjustable seal pair is configured to have a buffered contact with the other seal in the seal pair and is separated from the dispensing end along the elongated pin body; as well as A pair of adjusting members, connected to each pipette spindle and operably connected to the drive section, such that actuation of the drive section enables relative movement between the adjusting members and each pipette spindle connected to the adjusting members. The adjusting members collectively engage two seals in the pair, simultaneously adjusting each seal from a corresponding non-engaged position to an engaged position. In the non-engaged position, each seal forms a clearance fit between the corresponding pipette spindle and the corresponding pipette. In the engaged position, each seal contacts the inner peripheral surface of the corresponding pipette and cushions the reception of the corresponding pipette spindle, the inner peripheral surface fitting the corresponding pipette spindle. The two seals in the pair enable releasable radial gripping of the corresponding pipette fitted to the corresponding pipette spindle simultaneously.

2. The automated pipetting device according to claim 1, wherein, The simultaneous grasping of the corresponding pipette, which is mated to the corresponding pipette spindle, by using the two seals of the adjustable seal pair is achieved by a single actuation motion, which is common to each of the plurality of pipette spindles mated to the corresponding pipette.

3. The automated pipetting device according to claim 1, wherein, In the engagement position, the contact between the respective pipette tip and one of the seals in the pair of seals of the adjustable seal forms a continuous circumferential contact seal between the respective pipette spindle and the respective pipette tip mating thereto.

4. The automated pipetting device according to claim 3, wherein, When one of the seals is adjusted to the engagement position, a continuous circumferential contact seal is achieved.

5. The automated pipetting device according to claim 1, wherein, In the engagement position, the contact between the two seals in the corresponding pipette tip and the seal pair of the adjustable seal forms more than one continuous circumferential contact seal between the corresponding pipette spindle and the corresponding pipette tip mating thereto.

6. The automated pipetting device according to claim 5, wherein, When each seal in the pair of adjustable seals is adjusted to the engagement position, more than one continuous circumferential contact seal is achieved.

7. The automated pipetting device according to claim 5, wherein, More than one continuous circumferential contact seal is achieved simultaneously using only one actuation motion, which is common to each of the plurality of pipette mandrels.

8. The automated pipetting device according to claim 1, wherein, Each seal pair adjustment member connected to the plurality of pipette mandrels is operatively connected to a drive member common to another seal pair adjustment member, such that each of the seal pair adjustment members and the other seal pair adjustment member are actuated in unison by the common actuation action of the common drive member.

9. The automated pipetting device according to claim 1, wherein, The drive section includes a motor mounted to the frame and operably connected via a common screw to each seal pair adjustment member, which is coupled to the plurality of pipette mandrels.

10. The automated pipetting device according to claim 9, wherein, The motor is operably connected to each seal pair adjustment member via connecting rods, the connecting rods collectively actuating each seal pair adjustment member, the seal pair adjustment member being coupled to the plurality of pipette mandrels such that each seal pair of the adjustable seals coincides with another seal pair of the adjustable seals that grips another corresponding pipette, the pipettes being fitted into each of the plurality of pipette mandrels.

11. The automated pipetting device according to claim 1, wherein, The dispensing head is a 96 pipette dispensing head, which has a clearance fit with a clearance ratio of 1.02 to 1.

12. The automated pipetting device according to claim 1, wherein, The dispensing head is a 384 pipette dispensing head, which has a clearance fit with a clearance ratio of 1.11 to 1.

13. An automated pipetting method, comprising: Provide a framework; A drive section is provided, which is mounted to the frame; A dispensing head is provided, which is connected to the frame and has a plurality of pipette mandrels, each of the plurality of pipette mandrels having an elongated pin body having a dispensing end, each of the plurality of pipette mandrels being configured to mate with a corresponding pipette, the elongated pin body of the pipette mandrel being received in the corresponding pipette; A pair of adjustable seals is provided, the pair of seals being mounted to each pipette mandrel, wherein the pair of adjustable seals is configured such that one seal in the pair of adjustable seals has a cushioned contact with the other seal in the pair of adjustable seals and is separated from the dispensing end along the elongated pin body. A seal pair adjustment member is provided, which is coupled to each pipette spindle and operably connected to the drive section, such that actuation of the drive section enables relative movement between the seal pair adjustment member and each pipette spindle coupled to the seal pair adjustment member; as well as The two seals in the adjustable seal pair engage with the adjusting member of the seal pair, so that each seal in the adjustable seal pair is simultaneously adjusted from a corresponding non-engaged position to an engaged position. In the non-engaged position, each seal in the adjustable seal pair forms a clearance fit between a corresponding pipette spindle and a corresponding pipette. In the engaged position, each seal in the adjustable seal pair contacts the inner peripheral surface of the corresponding pipette and cushions the reception of the corresponding pipette spindle. The inner peripheral surface engages with the corresponding pipette spindle. By utilizing the two seals in the adjustable seal pair, a releasable radial gripping is achieved, simultaneously gripping the corresponding pipette engaged with the corresponding pipette spindle.

14. The automated pipetting method according to claim 13, wherein, The simultaneous grasping of the corresponding pipette, which is mated to the corresponding pipette spindle, by using the two seals of the adjustable seal pair is achieved by a single actuation motion, which is common to each of the plurality of pipette spindles mated to the corresponding pipette.

15. The automatic pipetting method according to claim 13, further comprising forming a continuous circumferential contact seal between the respective pipetting mandrel and the respective pipetting nozzle mated thereto by utilizing the contact between the respective pipetting nozzle and one of the seals in the pair of seals of the adjustable seal at the engagement position.

16. The automatic pipetting method according to claim 15 further includes, when one of the seals is adjusted to the engagement position, achieving continuous circumferential contact sealing.

17. The automated pipetting method of claim 13, further comprising forming more than one continuous circumferential contact seal between the respective pipette mandrel and the respective pipette mating thereto by utilizing the contact between the two seals in the respective pipette tip and the seal pair at the engagement position.

18. The automated pipetting method of claim 17 further comprises, when each seal in the pair of adjustable seals is adjusted to the engagement position, achieving more than one continuous circumferential contact seal.

19. The automated pipetting method of claim 17 further comprises, wherein more than one consecutive circumferential contact seal is achieved simultaneously by a single actuation motion, the actuation motion being common to each of the plurality of pipetting mandrels.

20. The automated pipetting method according to claim 13, wherein, Each seal pair adjustment member connected to the plurality of pipette mandrels is operatively connected to a drive member common to another seal pair adjustment member, such that each of the seal pair adjustment members and the other seal pair adjustment member are actuated in unison by the common actuation action of the common drive member.

21. The automated pipetting method of claim 13, further comprising providing a motor for the drive section, the motor being mounted to the frame and operably connected via a common screw to each seal pair adjustment member, the seal pair adjustment member being coupled to the plurality of pipetting tip mandrels.

22. The automatic pipetting method of claim 21, further comprising: operably connecting the motor to each pair of seal adjustment members via connecting rods, the connecting rods collectively actuating each pair of seal adjustment members, the pair of seal adjustment members being coupled to the plurality of pipette mandrels such that each pair of seals of the adjustable seals grasps the corresponding pipette in agreement with the seal pair of another adjustable seal that grasps another corresponding pipette, the pipettes engaging with each of the plurality of pipette mandrels.

23. The automated pipetting method according to claim 13, wherein, The dispensing head is a 96 pipette dispensing head, which has a clearance fit with a clearance ratio of 1.02 to 1.

24. The automated pipetting method according to claim 13, wherein, The dispensing head is a 384 pipette dispensing head, which has a clearance fit with a clearance ratio of 1.11 to 1.

25. A pipette tip for an automated pipetting device, the pipette tip comprising: A pipette collar, having an insertion end, is configured to intersect and automatically engage with the pipette mandrel of the automated pipetting device; A dispensing nozzle extends from the pipette collar and is opposite the insertion end; A first sealing surface is disposed on the pipette collar. The first sealing surface is configured to engage and buffer the insertion of the pipette spindle, such that the buffered contact between the first sealing surfaces causes the first sealing portion of the pipette spindle to close against the first sealing surface. A continuous circumferential contact seal is achieved by means of the radial tight contact between the first sealing surface and the pipette spindle. as well as A second sealing surface is disposed on the pipette collar such that the second sealing surface is longitudinally spaced from the first sealing surface. The second sealing surface is configured to engage and cooperate with a second sealing portion of the pipette spindle, thereby achieving a releasable grip and another continuous circumferential contact seal between the second sealing surface and the pipette spindle.

26. The pipette according to claim 25, wherein, As the pipette mandrel is inserted into the pipette collar, the first sealing surface forms a clearance fit with the pipette mandrel.

27. The pipette according to claim 25, wherein, As the pipette mandrel is inserted into the pipette collar, both the first sealing surface and the second sealing surface form a clearance fit with the pipette mandrel.

28. An automated pipetting method, comprising: A pipette tip for an automated pipetting device is provided, the pipette tip comprising: A pipette collar, having an insertion end, is configured to intersect and automatically engage with the pipette mandrel of the automated pipetting device; A dispensing nozzle extends from the pipette collar and is opposite the insertion end; The first sealing surface is disposed on the pipette collar, and A second sealing surface is disposed on the pipette collar such that the second sealing surface is longitudinally spaced from the first sealing surface; By utilizing the first sealing surface to engage and cushion the insertion of the pipette mandrel, the cushioned contact between the first sealing surfaces causes the first sealing portion of the pipette mandrel to close against the first sealing surface. Continuous circumferential contact sealing is achieved through the radially tight contact between the first sealing surface and the pipette mandrel; and The second sealing surface enables a releasable grip and another continuous circumferential contact seal between the second sealing surface and the pipette mandrel, wherein the second sealing surface mates with and engages the second sealing portion of the pipette mandrel.

29. The automated pipetting method according to claim 28, wherein, As the pipette mandrel is inserted into the pipette collar, the first sealing surface forms a clearance fit with the pipette mandrel.

30. The automated pipetting method according to claim 28, wherein, As the pipette mandrel is inserted into the pipette collar, both the first sealing surface and the second sealing surface form a clearance fit with the pipette mandrel.

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