Sample treatment detection device and sample treatment device

Abstract

The present invention relates to the technical field of in vitro diagnosis, and discloses a sample treatment detection device and a sample treatment device.The sample treatment device comprises: a sample treatment module, which is provided with a sample adding channel and a plurality of solution bins, at least one solution bin is internally provided with magnetic beads for adsorbing nucleic acid or protein, the bottom of the solution bin is communicated with a closed pipette, and the pipette is provided with a sealing gasket; the liquid driving device is arranged in the sample processing module, and a syringe needle at the bottom of the liquid driving device can be inserted into the sealing gasket; at least one of the liquid driving device and the solution bin moves relatively under the driving of the power mechanism, so that the syringe needle of the liquid driving module is inserted into the pipette channels corresponding to different solution bins. The sample treatment device is high in pipetting efficiency, capable of improving the detection efficiency, compact in structure, small in size and wide in application range.

Description

Technical Field

[0001] The present invention relates to the field of in vitro diagnostic technology, and more specifically, to a sample processing device. The present invention also provides a sample processing detection device comprising the sample processing device. Background Art

[0002] At present, in vitro diagnostic testing requires multiple pipetting of samples, reagents, reactants, etc. to complete the testing process. The large number of pipetting operations not only increases the workload but also reduces the testing efficiency.

[0003] In order to improve the efficiency of pipetting, a compressible hose is designed in the prior art, which is provided with a plurality of reaction areas for holding different reagents. The reaction areas are connected by thermal bonding to separate the reaction areas. When the reaction areas are compressed by mechanical force, the reagents in the reaction areas move under the action of mechanical squeezing, thereby realizing the movement of the reagents in different reaction areas. Although the above-mentioned compressible hose can improve the efficiency of pipetting, it can only perform 4-5 types of PCR tests. If multiple fluorescent quantitative PCR tests or CRP blood routine tests are required, the number of compressible hoses must be increased, or the number of reaction areas in the compressible hose must be increased, and the adaptability is poor.

[0004] In summary, how to develop a sample processing device that has a wide range of applications and can improve detection efficiency is an urgent problem to be solved by those skilled in the art. Summary of the invention

[0005] In view of this, an object of the present invention is to provide a sample processing device, which has high pipetting efficiency, can improve detection efficiency, and has a compact structure, a small size, and a wide range of adaptability.

[0006] In addition, the present invention also provides a sample processing detection device comprising the above sample processing device.

[0007] In order to achieve the above object, the present invention provides the following technical solutions:

[0008] A sample processing and detection device, comprising:

[0009] The sample processing module is provided with a sample loading channel and a plurality of solution chambers, at least one of the solution chambers is provided with magnetic beads for adsorbing nucleic acids or proteins, and the bottom of the solution chamber is connected to a closed pipetting pipeline, and the pipetting pipeline is provided with a sealing gasket;

[0010] A liquid driving module installed in the sample processing module, the syringe needle at the bottom of which can be inserted into the sealing gasket;

[0011] At least one of the liquid driving module and the solution tank moves relatively under the drive of the power mechanism, so as to enable the syringe needle of the liquid driving module to be inserted into the pipetting channels corresponding to different solution tanks.

[0012] Preferably, the liquid drive module includes a syringe mounting bracket and a syringe, the syringe is sleeved in the syringe mounting hole of the sample processing module through the syringe mounting bracket, the syringe mounting bracket is threadedly connected to the syringe mounting hole, and the syringe is clamped in the syringe mounting bracket.

[0013] Preferably, the syringe includes an eccentric syringe with an eccentrically arranged syringe needle, the eccentric syringe is arranged in the center of the sample processing module, the solution tank and the pipette pipe are evenly distributed along the circumferential direction of the sample processing module, and the pipette pipe is arranged on a side of the solution tank relatively close to the eccentric syringe.

[0014] Preferably, the sealing gasket used to seal each of the pipetting tubes is an integrally arranged annular sealing gasket, and the annular sealing gasket is provided with a downwardly recessed sealing portion at a position corresponding to each of the pipetting tubes.

[0015] Preferably, a limiting groove is provided at the bottom of the sample processing module, and a limiting portion for engaging with the limiting groove is provided at the outer edge of the annular sealing gasket.

[0016] Preferably, a rotary motor is provided at the bottom of the sample processing module, and the rotary motor can drive the sample processing module to step relative to the eccentric syringe, and the stepping angle of the rotary motor is the same as the central angle of two adjacent solution tanks.

[0017] Preferably, a sealing cover is provided on the top of the solution tank, and a filter element is provided inside the sealing cover, and the filter element is used to maintain the internal and external air pressure balance of the solution tank and prevent liquid from entering the solution tank.

[0018] Preferably, the sealing cover of each solution tank is an integrated structure.

[0019] A sample processing detection device comprises a sample processing component, a magnetron component and a detection module, wherein the sample processing component is any of the sample processing modules described above.

[0020] Preferably, the magnetic control assembly includes an electromagnet sleeved outside the solution tank, and when the electromagnet is energized, the electromagnet adsorbs the magnetic beads to the top of the solution tank to separate the magnetic beads from the solution;

[0021] When the electromagnet is powered off, the magnetic beads are located in the middle or bottom of the solution chamber, so that the magnetic beads are immersed in the solution.

[0022] The sample processing device provided by the present invention utilizes the relative movement of the liquid driving module and the sample processing module to insert the syringe needle into the liquid transfer pipe corresponding to the different solution compartments, thereby realizing the liquid transfer of different types of solutions. Compared with the existing mechanical extrusion liquid transfer method, the liquid transfer efficiency is high, the structure is simple, and the adaptability is strong;

[0023] The magnetic field is used to drive the magnetic beads to move in the solution chamber, changing the contact state between the magnetic beads and the solution, so that the attached substances on the surface of the magnetic beads can react with the solution, or the magnetic beads can be separated from the solution to transfer the solution in the solution chamber.

[0024] In addition, the present invention also provides a sample processing detection device comprising the above sample processing device. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the provided drawings without paying creative work.

[0026] Figure 1 A schematic diagram of the structure of a specific embodiment of the sample processing and detection device provided by the present invention;

[0027] Figure 2 for Figure 1 Schematic cross-sectional view in the AA direction;

[0028] Figure 3 This is a schematic diagram of the detection principle of the sample processing detection device provided by the present invention applied to CRP blood routine detection;

[0029] Figure 4 This is a schematic diagram of the detection principle of the sample processing detection device provided by the present invention applied to PCR detection.

[0030] Reference numerals:

[0031] 1-sample processing module; 11-solution chamber; 12-pipette pipeline; 2-syringe mounting bracket; 3-eccentric syringe; 4-sealing pad; 5-magnetic bead; 6-sample outlet; 7-magnetic control component. DETAILED DESCRIPTION

[0032] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0033] The core of the present invention is to provide a sample processing device, which has high liquid transfer efficiency, can improve detection efficiency, and has a compact structure, small size and a wide range of adaptability.

[0034] The present invention also provides a sample processing detection device comprising the sample processing device.

[0035] The sample processing and detection device provided by the present invention comprises:

[0036] The sample processing module 1 is provided with a sample addition channel and a plurality of solution chambers 11, at least one of the solution chambers 11 is provided with magnetic beads 5 for adsorbing nucleic acids or proteins, and the bottom of the solution chamber 11 is connected to a closed pipette pipe 12, and the pipette pipe 12 is provided with a sealing gasket 4;

[0037] A liquid driving module installed in the sample processing module 1, the syringe needle at the bottom of which can be inserted into the sealing gasket 4;

[0038] At least one of the liquid driving module and the sample processing module 1 moves relatively under the drive of the power mechanism, so that the syringe needle of the liquid driving module is inserted into the pipetting pipes 12 corresponding to different solution chambers 11 .

[0039] Among them, the sample processing module 1 is used to hold various reagents required for sample testing, such as lysis solution, cleaning solution, amplification solution, antibodies and luminescent substrates. In order to avoid reagent contamination, various reagents are respectively arranged in different solution tanks 11. The specific number and volume of the solution tanks 11 are determined according to the types and amounts of reagents required for in vitro diagnostic testing in actual production to ensure the normal progress of in vitro diagnostic testing.

[0040] Since nucleic acids or proteins need to be adsorbed and enriched in in vitro diagnostic tests for subsequent testing, at least one solution chamber 11 of the sample processing module 1 is provided with magnetic beads 5 for adsorbing nucleic acids or proteins. The magnetic beads 5 can be nucleic acid purification magnetic beads, immunomagnetic beads, etc. The specific type of magnetic beads 5 is determined according to the type of in vitro diagnostic test. For example, nucleic acid purification magnetic beads can be used for PCR testing, and immunomagnetic beads can be used for CRP routine blood testing.

[0041] The sample loading channel of the sample processing module 1 is connected to the solution chamber 11 provided with magnetic beads 5 so that the magnetic beads 5 can adsorb reactants such as nucleic acids or proteins obtained by the sample cleavage reaction; the sample loading channel can be set as an independent channel of the sample processing module 1, but it is preferred to set the sample loading channel and the solution chamber 11 as an integrated structure so as to simplify the structure of the sample processing module 1 and reduce production costs.

[0042] Taking into account the sample detection environment requirements, when the sample addition channel and the solution tank 11 are an integrated structure, preferably, a sealing cover can be provided on the top of the solution tank 11, and a filter element is provided in the sealing cover. The filter element is used to maintain the internal and external air pressure balance of the solution tank 11 and prevent liquid from entering the solution tank 11.

[0043] The specific materials of the sealing cover and the filter element are determined according to the actual detection needs and will not be repeated here; the sealing cover of each solution tank 11 can be set independently, and it is preferred to set the sealing cover of each solution tank 11 as an integrated structure to simplify the structure of the sample processing module 1 and facilitate processing and manufacturing.

[0044] In order to facilitate the taking and placing of the sealing cover, a protruding grip is provided on the outer edge of the sealing cover, which is used to facilitate the inspector to apply force to the sealing cover, thereby covering the sealing cover into the solution tank 11 or opening the sealing cover on the top of the solution tank 11.

[0045] The magnetic beads 5 can move in the solution chamber 11 under the drive of the magnetic field, changing the contact state between the magnetic beads 5 and the solution in the solution chamber 11, thereby facilitating the transfer of reagents and the reaction between the sample and the reagent. When the magnetic beads 5 move to the top of the solution chamber 11 under the action of the magnetic field, the magnetic beads 5 can be separated from the solution in the solution chamber 11, making it convenient for the syringe needle to absorb and transfer the solution in the solution chamber 11.

[0046] When the magnetic beads 5 move to the middle and lower part of the solution chamber 11 under the action of the magnetic field, the magnetic beads 5 are immersed in the solution of the solution chamber 11, and the nucleic acids, proteins or antigen-antibody reactants adsorbed by the magnetic beads 5 can react with the solution.

[0047] In order to realize the pipetting of reagents and reactants between different solution chambers 11, a pipetting pipe 12 sealed by a sealing gasket 4 is provided at the bottom of the solution chamber 11. At least one of the solution chambers 11 of the liquid driving module and the sample processing module 1 can move under the drive of a power mechanism, so that the liquid driving module and the sample processing module 1 move relative to each other, thereby allowing the syringe needle of the liquid driving module to be inserted into the pipetting pipe 12 corresponding to the different solution chambers 11.

[0048] The structure, size and distribution of the pipetting pipe 12 are determined according to the relative movement mode and movement range of the liquid driving module and the sample processing module 1 in actual production, so as to ensure that the syringe needle of the liquid driving module can be inserted into the pipetting pipe 12 corresponding to each solution tank 11 to perform pipetting on each solution tank 11.

[0049] The relative movement between the liquid driving module and the sample processing module 1 may be horizontal movement, rotational movement, or a combination of the two;

[0050] Taking into account the layout and volume of the sample processing and detection device, it is preferred to set the liquid driving module and the sample processing module 1 to rotate relative to each other. At this time, the syringe of the liquid driving module can be arranged on the inner side of each solution tank 11 of the sample processing module 1, which not only makes the sample processing and detection device compact, but also reduces the moving distance of the syringe, which is conducive to improving the pipetting efficiency and thus improving the detection operation efficiency.

[0051] For example, see Figure 2 The syringe includes an eccentric syringe 3 with an eccentrically arranged syringe needle. The eccentric syringe 3 is arranged in the center of the sample processing module 1. The solution chamber 11 and the pipette pipeline 12 are evenly distributed along the circumferential direction of the sample processing module 1. The pipette pipeline 12 is arranged on a side of the solution chamber 11 relatively close to the eccentric syringe 3.

[0052] The liquid driving module includes a syringe mounting bracket 2 and a syringe. In order to stably install the syringe in the sample processing module 1, it is preferred to arrange the syringe to be sleeved in the syringe mounting hole of the sample processing module 1 through the syringe mounting bracket 2. The syringe mounting bracket 2 is threadedly connected to the syringe mounting hole, and the syringe is clamped in the syringe mounting bracket 2.

[0053] The size of the syringe mounting bracket 2 is determined according to the size of the syringe mounting hole of the sample processing module 1 and the size of the syringe in actual production, and will not be described in detail here.

[0054] Taking into account the overall layout of the sample processing and detection device, a sample outlet 6 is further provided at the bottom of the sample processing module 1. The sample outlet 6 corresponds to the solution tank 11 which serves as a sample and reagent reaction container and can cooperate with the detection module for detection in the sample outlet 6, or enter the detection module through the sample outlet 6 for detection.

[0055] The type and model of the power mechanism is determined according to the relative movement mode of the liquid drive module and the sample processing module 1. For example, when the two move relatively horizontally, the power mechanism can be set as a linear motor, an electric push rod, etc.; when the two rotate relatively, the power mechanism can be set as a servo motor, a stepper motor, etc.

[0056] In this embodiment, the relative movement of the liquid driving module and the sample processing module 1 is utilized to insert the syringe needle into the pipette pipe 12 corresponding to the different solution chambers 11, thereby realizing the pipetting of different types of solutions. Compared with the existing mechanical squeezing pipetting method, the pipetting efficiency is high, the structure is simple, and the adaptability is strong;

[0057] The magnetic field is used to drive the magnetic beads 5 to move in the solution chamber 11, changing the contact state between the magnetic beads 5 and the solution, so that the attached substances on the surface of the magnetic beads 5 contact and react with the solution, or the magnetic beads 5 are separated from the solution to transfer the solution in the solution chamber 11.

[0058] On the basis of the above embodiment, in order to effectively seal the pipette pipe 12 , the sealing gasket 4 used to seal each pipette pipe 12 is an integrally arranged annular sealing gasket, and the annular sealing gasket is provided with a downwardly recessed sealing portion at the position corresponding to each pipette pipe 12 .

[0059] The material of the annular sealing gasket is determined according to the seals used in common in vitro diagnostic testing equipment in actual production to ensure its sealing performance and corrosion resistance. The radius of the annular sealing gasket should be greater than or equal to the distance from the inner side of the solution chamber 11 to the sample processing module 1;

[0060] The shape and size of the sealing portion are determined according to the shape and size of the pipetting tube 12 in actual production.

[0061] In this embodiment, an integrally arranged annular sealing gasket is used to seal each pipetting pipe 12 at the same time. Compared with each sealing gasket 4 independently sealing each pipetting pipe 12, the contact area between the annular sealing gasket and the sample processing module 1 is large, the sealing performance is better, and the assembly process of the sealing gasket 4 is simpler.

[0062] Preferably, in order to further improve the sealing performance of the annular sealing gasket, a limiting groove may be provided at the bottom of the sample processing module 1, and a limiting portion for engaging with the limiting groove may be provided at the outer edge of the annular sealing gasket.

[0063] The above-mentioned limiting groove can be an arc groove, but it is preferably set as an annular groove. The sealing area of ​​the annular groove is larger than that of the arc groove and the sealing effect is better; the specific cross-sectional structure and size of the limiting groove are determined according to the needs in actual production and will not be repeated here.

[0064] On the basis of the above embodiment, when the solution chamber 11 and the corresponding pipette pipe 12 are evenly distributed along the circumferential direction of the sample processing module 1, in order to drive the sample processing module 1 to rotate, a rotating motor is provided at the bottom of the sample processing module 1. The rotating motor can drive the sample processing module 1 to step relative to the eccentric syringe 3, and the stepping angle of the rotating motor is the same as the central angle corresponding to the two adjacent solution chambers 11.

[0065] In this embodiment, a rotary motor is used to drive the sample processing module 1 to rotate and step, which not only facilitates the setting of the power mechanism and makes the power connection structure simple, but also ensures that the eccentric syringe 3 and the sealing gasket 4 are accurately aligned after stepping, avoiding the problem of the eccentric syringe 3 failing to be inserted into the pipetting tube 12.

[0066] In addition to the above-mentioned sample processing device, the present invention also provides a sample processing detection device including the sample processing device disclosed in the above-mentioned embodiment, the sample processing detection device includes a sample processing component, a magnetron component 7 and a detection module, and the sample processing component is the sample processing device disclosed in the above-mentioned embodiment.

[0067] The magnetron assembly 7 is used to generate a magnetic field and utilize the change of the magnetic field to drive the magnetic beads 5 to move in the solution chamber 11 of the sample processing module 1. The change of the magnetic field can be either a change in the magnetic field intensity or a change in the magnetic field direction.

[0068] For example, the magnetic control assembly 7 may include an electromagnet sleeved outside the solution chamber 11. When the electromagnet is energized, the electromagnet adsorbs the magnetic beads 5 to the top of the solution chamber 11, so that the magnetic beads 5 are separated from the solution.

[0069] When the electromagnet is powered off, the magnetic beads 5 are located in the middle or bottom of the solution chamber 11 , so that the magnetic beads 5 are immersed in the solution.

[0070] The specific type and power of the electromagnet are determined according to the material, size and quantity of the magnetic beads 5 in actual production to ensure that the magnetic field generated by the electromagnet can absorb all the magnetic beads 5 .

[0071] The magnetic control component 7 uses an electromagnet as the magnetic control component 7, and controls the presence or absence of a magnetic field by energizing and energizing the electromagnet, thereby driving the magnetic beads 5 to move in the solution chamber 11, with a simple structure and convenient control.

[0072] In addition to changing the power-on state of the electromagnet to control the presence or absence of the magnetic field, the strength of the magnetic field can also be affected by moving the position of the magnetic field. For example, a linear motion mechanism such as a linear motor is set to drive the electromagnet or ordinary magnet to move relative to the solution chamber 11 in the height direction or radial direction, and the adsorption and release of the magnetic beads 5 are achieved by the approach and distance of the electromagnet or ordinary magnet.

[0073] Of course, the magnetic field of the magnetron assembly 7 may also be set to always exist, and the direction of the magnetic field may be switched to change the direction of the magnetic field force on the magnetic beads 5 , thereby driving the magnetic beads 5 to move in the solution chamber 11 .

[0074] The structure and distribution of the detection module need to be determined according to the function of the sample processing detection device. For example, in CRP blood routine testing, PMT elements (photomultiplier tubes) are used to perform fluorescence detection of proteins, and in multiplex fluorescence quantitative PCR testing, MPPC detection elements (multi-pixel photon counters) are used to perform multiplex fluorescence measurements of PCR.

[0075] In a specific embodiment, please refer to Figure 3 Six solution chambers 11 are provided in the sample processing module 1, and lysis solution, a first cleaning solution, an antibody, a second cleaning solution and a luminescent substrate are sequentially contained in five solution chambers 11. The solution chamber 11 not containing reagents is used as a waste liquid chamber, and a PMT element is provided outside the sample outlet 6 at the bottom of the sample processing module 1.

[0076] When testing is required, the sample enters the No. 1 solution chamber containing the lysis solution through the sample loading channel, and the sample is lysed under the action of the lysis solution to produce a C-reactive protein structure;

[0077] After the lysis is completed, the magnetic control assembly 7 is controlled to move the magnetic beads 5 to the top of the No. 1 solution bin, and then the eccentric syringe 3 is controlled to absorb the lysis solution in the No. 1 solution bin and transfer it to the waste liquid bin, and then the eccentric syringe 3 is controlled to absorb the first cleaning solution in the No. 2 solution bin and transfer it to the No. 1 solution bin;

[0078] After the first cleaning solution is pipetted, the magnetic control assembly 7 is controlled to move the magnetic beads 5 to the middle and lower part of the No. 1 solution compartment, and the lysed C-reactive protein is cleaned with the first cleaning solution to remove the lysate remaining on the protein surface;

[0079] After the cleaning is completed, the magnetic control assembly 7 is controlled to move the magnetic beads 5 to the top of the No. 1 solution bin, and then the eccentric syringe 3 is controlled to absorb the cleaning solution in the No. 1 solution bin and transfer it to the waste liquid bin, and then the eccentric syringe 3 is controlled to absorb the antibody in the No. 3 solution bin and transfer it to the No. 1 solution bin;

[0080] After the antibody is pipetted, the magnetic control assembly 7 is controlled to move the magnetic beads 5 to the middle and lower part of the No. 1 solution compartment, so that the washed C-reactive protein reacts with the antibody to form an antigen-antibody complex;

[0081] After the reaction is completed, the magnetic control assembly 7 is controlled to move the magnetic beads 5 to the top of the No. 1 solution bin, and then the eccentric syringe 3 is controlled to absorb the antibody solution in the No. 1 solution bin and transfer it to the waste liquid bin, and then the eccentric syringe 3 is controlled to absorb the second cleaning solution in the No. 4 solution bin and transfer it to the No. 1 solution bin;

[0082] After the transfer of the second cleaning solution is completed, the magnetic control assembly 7 is controlled to move the magnetic beads 5 to the middle and lower part of the No. 1 solution chamber, and the antigen-antibody conjugate is cleaned with the second cleaning solution to remove the residual antibodies on the surface of the antigen-antibody conjugate;

[0083] After the cleaning is completed, the magnetic control assembly 7 is controlled to move the magnetic beads 5 to the top of the No. 1 solution bin, and then the eccentric syringe 3 is controlled to absorb the cleaning liquid in the No. 1 solution bin and transfer it to the waste liquid bin, and then the eccentric syringe 3 is controlled to absorb the luminescent substrate in the No. 5 solution bin and transfer it to the No. 1 solution bin;

[0084] After the transfer of the luminescent substrate is completed, the magnetic control assembly 7 is controlled to move the magnetic beads 5 to the middle and lower part of the No. 1 solution compartment, so that the antigen-antibody conjugate is combined with the luminescent substrate for subsequent fluorescence detection;

[0085] After the reaction is completed, the magnetic control component 7 is controlled to move the magnetic beads 5 to the top of the No. 1 solution tank, and then the eccentric syringe 3 is controlled to absorb the luminescent substrate in the No. 1 solution tank and transfer it to the waste liquid tank, and then the PMT element is used to irradiate the reactants in the sample outlet 6 for fluorescence detection.

[0086] In another specific embodiment, six solution tanks 11 are provided in the sample processing module 1, and the five solution tanks 11 contain lysis solution, first cleaning solution, second cleaning solution, elution solution and amplification solution in sequence. The sample loading channel is connected to the solution tank 11 containing the lysis solution. An amplification tube is provided outside the sample outlet 6 at the bottom of the sample processing module 1, and the other end of the amplification tube is connected to the sample inlet of the detection module so that the MPPC detection element can perform multiple fluorescence measurements on the PCR after amplification.

[0087] It should be noted that the above-mentioned lysis solution, first cleaning solution and second cleaning solution are only used to demonstrate the functions of the reagents; in different types of tests, the types of lysis solution, first cleaning solution and second cleaning solution used may be different, and the specific types of each reagent are determined according to actual test needs.

[0088] The sample processing and detection device can perform nucleic acid lysis, cleaning, elution, drying and amplification on the sample, transfer the amplified reactants to the amplification tube through the sample outlet 6, and then perform multiple fluorescence quantitative detection on them using the MPPC detection element.

[0089] It should be noted that the first cleaning solution and the second cleaning solution mentioned in the present application document are only used to distinguish the types of reagents and do not limit the order.

[0090] The various embodiments in this specification are described in a progressive manner, and each embodiment focuses on the differences from other embodiments. The same or similar parts between the various embodiments can be referenced to each other.

[0091] The sample processing detection device and the sample processing device provided by the present invention are introduced in detail above. Specific examples are used herein to illustrate the principles and implementation methods of the present invention. The description of the above embodiments is only used to help understand the method of the present invention and its core idea. It should be pointed out that for ordinary technicians in this technical field, without departing from the principle of the present invention, the present invention can also be improved and modified in a number of ways, and these improvements and modifications also fall within the scope of protection of the claims of the present invention.

Claims

1. A sample processing device, characterized in that: include: A sample processing module (1) is provided with a sample loading channel and a plurality of solution chambers (11), at least one of the solution chambers (11) being provided with magnetic beads (5) for adsorbing nucleic acids or proteins, and the bottom of the solution chamber (11) is connected to a closed liquid transfer pipe (12), and the liquid transfer pipe (12) is provided with a sealing gasket (4); A liquid drive module installed in the sample processing module (1), wherein the syringe needle at the bottom of the liquid drive module can be inserted into the sealing gasket (4); At least one of the liquid driving module and the sample processing module (1) moves relative to each other under the drive of a power mechanism, so that the syringe needle of the liquid driving module is inserted into the pipetting channel (12) corresponding to different solution chambers (11).

2. The sample processing device according to claim 1, characterized in that: The liquid drive module comprises a syringe mounting frame (2) and a syringe, the syringe being sleeved in a syringe mounting hole of the sample processing module (1) via the syringe mounting frame (2), the syringe mounting frame (2) being threadedly connected to the syringe mounting hole, and the syringe being clamped in the syringe mounting frame (2).

3. The sample processing device according to claim 2, characterized in that: The syringe comprises an eccentric syringe (3) with an eccentrically arranged syringe needle, the eccentric syringe (3) being arranged at the center of the sample processing module (1), the solution chamber (11) and the pipetting pipeline (12) being evenly distributed along the circumferential direction of the sample processing module (1), and the pipetting pipeline (12) being arranged on a side of the solution chamber (11) relatively close to the eccentric syringe (3).

4. The sample processing device according to claim 3, characterized in that: The sealing gasket (4) used for sealing each of the pipetting pipes (12) is an integrally arranged annular sealing gasket, and the annular sealing gasket is provided with a downwardly recessed sealing portion at a position corresponding to each of the pipetting pipes (12).

5. The sample processing device according to claim 4, characterized in that: A limiting groove is provided at the bottom of the sample processing module (1), and a limiting portion for engaging with the limiting groove is provided at the outer edge of the annular sealing gasket.

6. The sample processing device according to claim 3, characterized in that: A rotary motor is provided at the bottom of the sample processing module (1), and the rotary motor can drive the sample processing module (1) to step relative to the eccentric syringe (3), and the stepping angle of the rotary motor is the same as the central angle of two adjacent solution chambers (11).

7. The sample processing device according to any one of claims 1 to 6, characterized in that: A sealing cover is provided on the top of the solution tank (11), and a filter element is provided inside the sealing cover. The filter element is used to maintain the balance of internal and external air pressures of the solution tank (11) and prevent liquid from entering the solution tank (11).

8. The sample processing device according to claim 7, characterized in that: The sealing cover of each solution tank (11) is an integrated structure.

9. A sample processing and detection device, characterized in that: It comprises a sample processing component, a magnetron component (7) and a detection module, wherein the sample processing component is the sample processing device according to any one of claims 1 to 8.

10. The sample processing and detection device according to claim 9, characterized in that: The magnetic control component (7) comprises an electromagnet sleeved outside the solution chamber (11); when the electromagnet is energized, the electromagnet adsorbs the magnetic beads (5) to the top of the solution chamber (11), thereby separating the magnetic beads (5) from the solution; When the electromagnet is powered off, the magnetic beads (5) are located in the middle or bottom of the solution chamber (11), so that the magnetic beads (5) are immersed in the solution.

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