A thermostatic patch clamp cell observation and recording system for cell experiments

Abstract

The invention discloses a constant temperature patch clamp cell observation and recording system for cell experiments, which relates to the technical field of cell observation and comprises: a microscope; a constant temperature bath, which is placed on the microscope and used to provide a constant temperature environment; a cell bath, which is placed in the middle of the constant temperature bath and used to carry cells to be detected; a microelectrode controller, which is arranged on one side of the microscope and used to contact the cells to be detected and provide a clamping voltage; and a drug delivery system, which is used to provide a drug solution environment for the cells to be detected in the cell bath; wherein the constant temperature bath comprises: a constant temperature bath body, which is made of a transparent material and has a lifting groove formed by an upward depression in the middle; at least two symmetrically arranged positioning cylinders, which are fixed to the inner side of the constant temperature bath body and used to support the cell bath; and a liquid inlet and a liquid outlet connected to the side of the constant temperature bath body, wherein the liquid inlet and the liquid outlet are symmetrically arranged and connected to an external constant temperature water circulation device.

Description

Technical Field

[0001] The invention relates to the technical field of cell observation, in particular to a constant temperature patch clamp cell observation and recording system for cell experiments. Background Art

[0002] The patch clamp cell observation and recording system is a key experimental technology for studying cell membrane ion channels. Ion channels, as proteins on the cell membrane, finely regulate the entry and exit of ions. By capturing the changes in weak electrical signals on the cell membrane, this technology can accurately monitor the opening and closing of ion channels and reveal their physiological functions and regulatory mechanisms.

[0003] To ensure the accuracy and reliability of the experimental results, this process is usually carried out under constant temperature conditions. However, the temperature control effect of the existing constant temperature bath technology on the cell bath depends to a large extent on the constant temperature water flow in the constant temperature bath. The rapid renewal and replacement of the water flow is the key, but excessive water flow will have an impact on the cell bath and affect the stability of the cells. There are currently two ways to solve this problem, but both have their limitations: although increasing the depth of the constant temperature bath can reduce the impact of water flow, it increases the distance between the inverted microscope and the cell bath, affecting the convenience of observation; although enhancing the connection strength between the cell bath and the constant temperature bath can be achieved through bolt connection, the installation process is cumbersome and may introduce impurities, reducing experimental efficiency.

[0004] In addition, the impact of temperature changes on experimental results cannot be ignored. In the patch clamp cell observation and recording system, temperature fluctuations may profoundly affect the physiological state of cells and ion channel functions. For example, temperature changes can affect the opening and closing of ion channels, further affecting the potential and current of cells. Therefore, it is crucial to design an experimental device that can quickly adjust the temperature to conduct temperature change experiments in the patch clamp cell observation and recording system. This device must have the ability to quickly stabilize within the set temperature range and be able to accurately control temperature changes. At the same time, it must also have good thermal insulation performance and simple operability to facilitate the operation and use of experimenters.

[0005] In summary, it is urgent and necessary to develop a constant temperature patch clamp cell observation and recording system for cell experiments. Summary of the invention

[0006] To achieve the above object, the present invention provides the following technical solution: a thermostatic patch clamp cell observation and recording system for cell experiments, comprising:

[0007] A microscope is placed on an anti-vibration table, and a shielding net is arranged outside the anti-vibration table;

[0008] A constant temperature bath, which is placed on the microscope and is used to provide a constant temperature environment;

[0009] A cell bath, which is placed in the middle of the constant temperature bath and is used to carry cells to be detected;

[0010] A microelectrode controller, which is disposed on one side of the microscope and is used to contact the cells to be detected and provide a clamping voltage; and

[0011] A drug delivery system, used for providing a drug solution environment for the cells to be tested in the cell bath;

[0012] Wherein, the constant temperature bath comprises:

[0013] The constant temperature tank body is made of transparent material, and the middle part is concave upward to form a lifting groove;

[0014] at least two symmetrically arranged positioning cylinders, which are fixed to the inner side of the thermostatic bath body and are used to support the cell bath; and

[0015] The liquid inlet and outlet holes are connected to the side of the thermostatic tank body, the liquid inlet and outlet holes are symmetrically arranged, and are connected to an external thermostatic water circulation device.

[0016] Further, preferably, a water bath space is formed between the lifting tank and the inner wall of the constant temperature tank body, and a baffle is provided in the middle of the water bath space, and the baffle can separate the water bath space into an outer water bath space and an inner water bath space.

[0017] Further, preferably, the top of the lifting groove has an arc angle.

[0018] Furthermore, preferably, the constant temperature tank body, lifting tank and enclosure are all circular structures.

[0019] Further, as a preference, an attachment bin is fixed at the bottom of the thermostatic tank body, and a lifting rod is fixed in the attachment bin for lifting and lowering the enclosure;

[0020] The lifting rod is configured such that when the temperature of the thermostatic bath is adjusted quickly, the lifting rod drives the enclosure to descend so that the bottom of the enclosure contacts the inner lower surface of the thermostatic bath body. At this time, the external thermostatic water circulation device extracts all the water in the external water bath space, and then injects water of the first temperature into the external water bath space. At this time, the lifting rod drives the enclosure to rise slowly.

[0021] The enclosure is made of heat-conducting material.

[0022] Further, preferably, when the cell bath is carried by the positioning cylinder in the constant temperature bath, there is a first distance between the bottom of the cell bath and the upper surface of the lifting groove, and the first distance is less than 8 mm.

[0023] Furthermore, preferably, the liquid level in the constant temperature bath is lower than the lowest position of the upper surface of the enclosure and higher than the bottom of the cell bath.

[0024] Further, preferably, the cell bath comprises:

[0025] A cell tank body, the outer diameter of which is smaller than the outer diameter of the lifting tank;

[0026] A clamping column, which can be clamped into the positioning cylinder; and

[0027] An extension rod connected between the clamping column and the cell tank body.

[0028] Further, as a preference, the positioning tube comprises:

[0029] A column groove is provided on the upper surface of the positioning cylinder and is used for clamping the clamping column;

[0030] An elastic cylinder, which is attached to the inner surface of the column groove and is used to lock the clamping column;

[0031] a plurality of expansion grooves, which are formed on the lower surface of the positioning tube and communicate with the column groove; and

[0032] An expansion column embedded in the expansion groove, the expansion column being expandable by absorbing water;

[0033] A locking pad is provided at the connection point between the expansion groove and the column groove, and the locking pad is made of elastic and water-impermeable material.

[0034] Compared with the prior art, the present invention provides a thermostatic patch clamp cell observation and recording system for cell experiments, which has the following beneficial effects:

[0035] In the embodiment of the present invention, by optimizing the structure of the constant temperature bath, the rapid update and replacement of the constant temperature water flow is ensured. Even if the water flow is large, it will not have a significant impact on the cell bath, thereby improving the stability of the cells and ensuring the convenience of observation. In addition, in the subsequent study of the relationship between temperature and cell changes, the optimized constant temperature bath can also play a greater role in assisting. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] Figure 1 A schematic diagram of a thermostatic patch clamp cell observation and recording system for cell experiments;

[0037] Figure 2 A schematic diagram of the three-dimensional structure of a thermostatic bath in a thermostatic patch clamp cell observation and recording system for cell experiments Figure 1 ;

[0038] Figure 3 A schematic diagram of the three-dimensional structure of a thermostatic bath in a thermostatic patch clamp cell observation and recording system for cell experiments Figure 2 ;

[0039] Figure 4 A schematic diagram of the cross-sectional structure of a thermostatic bath in a thermostatic patch clamp cell observation and recording system for cell experiments;

[0040] Figure 5 A schematic diagram of the structure of a cell bath in a thermostatic patch clamp cell observation and recording system for cell experiments;

[0041] Figure 6 Schematic diagram of patch clamp cell observation;

[0042] In the figure: 1. microscope; 2. constant temperature bath; 3. cell bath; 4. microelectrode controller; 5. drug delivery system; 6. auxiliary system; 21. constant temperature bath body; 22. positioning cylinder; 23. liquid inlet hole; 24. liquid outlet hole; 25. lifting slot; 26. arc angle; 27. enclosure; 28. attachment chamber; 31. cell bath body; 32. extension rod; 33. clamping column; 221. column slot; 222. elastic cylinder; 223. locking pad; 224. expansion column. DETAILED DESCRIPTION

[0043] Example: Please refer to Figure 1-Figure 6 In an embodiment of the present invention, a constant temperature patch clamp cell observation and recording system for cell experiments is provided, comprising:

[0044] Microscope 1 is placed on an anti-vibration platform, and a shielding net is arranged outside the anti-vibration platform. In the constant temperature patch clamp cell observation and recording system for cell experiments, the main function of the shielding net is to prevent external interference signals from affecting the experimental results. Specifically, it can shield interference signals such as mobile phone signals, play a role in noise reduction, and thus ensure the accuracy and reliability of the experimental results;

[0045] The constant temperature bath 2 is placed on the microscope 1 to provide a constant temperature environment. The constant temperature environment refers to a constant temperature environment. Generally speaking, the temperature fluctuation range does not exceed ±0.5°C. In a constant temperature environment;

[0046] A cell bath 3, which is placed in the middle of the constant temperature bath 2 and is used to carry cells to be detected;

[0047] A microelectrode controller 4, which is disposed on one side of the microscope 1 and is used to contact the cells to be detected and provide a clamping voltage; and

[0048] A drug delivery system 5, used for providing a drug solution environment for the cells to be detected in the cell bath 3;

[0049] Wherein, the constant temperature bath 2 comprises:

[0050] The constant temperature tank body 21 is made of transparent material, and the middle part is concave upward to form a lifting groove 25;

[0051] At least two symmetrically arranged positioning cylinders 22, which are fixed to the inner side of the thermostatic tank body 21 and used to support the cell bath 3; and

[0052] The liquid inlet 23 and the liquid outlet 24 connected to the side of the thermostatic tank 21 are symmetrically arranged and connected to an external constant temperature water circulation device, which is an important component of the constant temperature patch clamp cell observation and recording system for cell experiments. Its main function is to circulate constant temperature water and heat or cool the constant temperature water to ensure that the temperature of the supplied constant temperature water remains consistent. The external constant temperature water circulation device plays an important role in the experimental process. It can provide a constant temperature experimental environment, which is crucial to the accuracy and reliability of the patch clamp experiment.

[0053] External constant temperature water circulation equipment usually consists of heating and cooling parts, which can heat or cool the liquid according to the experimental requirements. The heating part usually uses electric heating elements or microwave heating technology, while the cooling part uses liquid nitrogen or refrigerants, which will not be described here;

[0054] In the process of circulating the constant temperature water, the constant temperature water in the constant temperature bath 2 is first sucked into the external constant temperature water circulation device, and then processed by the heating or cooling part. The treated constant temperature water is sent back to the constant temperature bath 2 through the circulation pump to ensure that the temperature of the supplied constant temperature water remains consistent.

[0055] In addition, in order to accurately control the temperature, the external constant temperature water circulation equipment is usually equipped with a temperature sensor and a control system. The temperature sensor monitors the temperature of the constant temperature water in real time and feeds the data back to the control system. The control system compares the set temperature value with the feedback temperature data, and then adjusts the power of the heating or cooling part to maintain a constant constant temperature of the water.

[0056] Generally speaking, the thermostatic patch clamp cell observation and recording system used in cell experiments includes single cell recording system and brain slice recording system, and there are some differences between the two in terms of equipment composition;

[0057] In this embodiment, a single cell recording system can be taken as an example. At this time, the microscope 1 is an inverted microscope. Based on this, the constant temperature patch clamp cell observation and recording system for cell experiments also includes an auxiliary system 6, which includes a patch clamp amplifier, a digital-to-analog / analog-to-digital converter, data acquisition and analysis software, a computer, a microscope widening platform (for placing the microelectrode controller 4), a micromanipulator, etc., which will not be repeated here;

[0058] It needs to be explained that the temperature control effect of the constant temperature bath 2 on the cell bath 3 depends on the flow rate of its constant temperature water. Rapid exchange of new and old constant temperature water is the key to ensuring temperature stability. However, excessive flow of constant temperature water may have an impact on the cell bath 3 and affect the stability of the cells therein. To solve this problem, there are usually two methods: one is to increase the depth of the constant temperature bath 2 to reduce the fluctuations caused by the water flow; but this method will cause the distance between the inverted microscope and the cell bath 3 to increase, affecting the convenience of observation. The second is to enhance the connection strength between the cell bath 3 and the constant temperature bath 2, generally using bolts, but the installation process of this method is more cumbersome, may introduce impurities, affect the drug solution environment, and is not efficient.

[0059] In this embodiment, in the current system, we increase the depth of the constant temperature tank body 21 and make the middle part of it concave upward to form a lifting tank. This design not only shortens the distance between the inverted microscope and the cell bath 3, making observation more convenient, but also effectively avoids the influence of water flow fluctuations on the stability of cells in the cell bath 3. This improvement optimizes the convenience of experimental operation and the clarity of observation while retaining the constant temperature performance, greatly improving the efficiency and quality of the experiment.

[0060] In this embodiment, the working process of the thermostatic patch clamp cell observation and recording system for cell experiments mainly includes the following steps:

[0061] 1. Prepare experimental materials: Prepare cell membranes and ensure that they are cultured in appropriate solutions. At the same time, prepare various required equipment and reagents, such as patch clamps, electrodes, cell clamps, etc.

[0062] 2 Install experimental equipment: Install the patch clamp, microscope and other equipment as required and ensure they are in good working condition.

[0063] 3. Preparation of cell membrane sheets: Take the cells out of the culture dish and use a micropipette to adsorb them onto a glass micropipette to form a cell membrane sheet.

[0064] 4. Voltage clamping: Voltage clamping is performed on the cell membrane to keep it at a specific voltage state.

[0065] 5. Observation and recording: Under voltage clamping, observe the cell membrane patch through a microscope and use a patch clamp to record the electrical signals on the membrane patch. These electrical signals can reflect the activity of ion channels on the cell membrane.

[0066] 6. Data processing: Process and analyze the recorded electrical signals, such as filtering and amplification, to obtain more accurate experimental results.

[0067] 7. Result analysis: Statistical analysis is performed on the processed data to obtain the experimental results. Various statistical analysis methods can be used to evaluate the experimental results as needed.

[0068] In this embodiment, the connection mode between the cell bath 3 and the constant temperature bath 2 is also optimized. Specifically, in this embodiment, the cell bath 3 includes:

[0069] The cell tank body 31 has an outer diameter smaller than the outer diameter of the lifting tank 25;

[0070] A clamping column 33, which can be clamped into the positioning cylinder 22; and

[0071] An extension rod 32 is connected between the clamping column 33 and the cell tank 31 .

[0072] The positioning tube 22 includes:

[0073] The column groove 221 formed on the upper surface of the positioning tube 22 is used to clamp the clamping column 33;

[0074] An elastic tube 222, which is attached to the inner surface of the column groove 221 and is used to lock the clamping column 33;

[0075] A plurality of expansion grooves are provided on the lower surface of the positioning tube 22 and are connected to the column groove 221; and

[0076] An expansion column 224 embedded in the expansion groove, wherein the expansion column 224 can expand by absorbing water;

[0077] A locking pad 223 is provided at the connection point between the expansion groove and the column groove 221 and is made of elastic and water-impermeable material.

[0078] In actual operation, we have taken a number of measures to ensure the stability of the cell tank. First, four positioning cylinders 22 are configured, which are distributed in a cross shape. In this way, the movement of the cell tank 31 in the horizontal direction is effectively restricted. More importantly, through the cooperation of the clamping column 33 and the elastic cylinder 222, we further enhance the restriction on the cell tank in the vertical direction. The elastic cylinder 222 is close to the inner surface of the column groove 221, has excellent elasticity, and can firmly lock the clamping column 33, thereby ensuring the stability of the cell tank 31 in the vertical direction.

[0079] It is worth mentioning that the structure of the positioning tube 22 is also optimized. The specific structure of the positioning tube 22 is as follows:

[0080] The column groove 221 is formed on the upper surface of the positioning tube, and provides a stable clamping position for the clamping column 33 .

[0081] The elastic tube 222 is tightly fitted to the inner wall of the column groove 221 , and its key function is to lock the clamping column 33 and enhance its stability.

[0082] Expansion grooves: are provided on the lower surface of the positioning tube and communicate with the column grooves 221. These expansion grooves provide space for the expansion column 224 to be embedded.

[0083] Expansion column 224: This special expansion column 224 has the characteristic of swelling by absorbing water. When constant temperature water is injected into the constant temperature bath 2, the expansion column 224 will absorb water and swell, further squeezing the locking pad 223. The locking pad 223 is made of elastic and impermeable material to ensure that it can firmly lock the clamping column 33, thereby greatly reducing the possibility of shaking of the cell tank 31 in the vertical direction.

[0084] Through this series of optimization measures, not only the stability of the cell tank is enhanced, but also the experimental operation is made simpler and more efficient. This design not only ensures the accuracy of the experiment, but also greatly improves the experimental efficiency, bringing great convenience to scientific researchers.

[0085] In this embodiment, in order to further reduce the impact of the constant temperature water flow on the cell bath 3, the top of the lifting tank 25 is specially designed to be an arc angle 26. This arc design not only enhances the stability of the lifting tank in structure, but also more effectively disperses the impact force of the water flow, and reduces the interference of the water flow on the cells in the cell bath 3. The structure of the arc angle 26 not only optimizes the hydrodynamic characteristics of the system, but also significantly improves the survival rate of cells and the accuracy of experiments in practical applications. The details of this design fully reflect the unique thinking and rigorous consideration of this system in improving experimental efficiency and ensuring experimental quality.

[0086] In this embodiment, a water bath space is formed between the lifting tank 25 and the inner wall of the constant temperature tank body 21. A baffle 27 is provided in the middle of the water bath space. The baffle 27 can separate the water bath space into an outer water bath space and an inner water bath space.

[0087] The thermostatic tank body 21, the lifting tank 25 and the enclosure 27 are all circular structures. In this embodiment, a water bath space is constructed between the lifting tank 25 and the inner wall of the thermostatic tank body 21. A enclosure 27 is provided in the middle of the water bath space, which serves to clearly separate the water bath space into an outer water bath space and an inner water bath space. This design not only increases the functionality of the system, but also improves the accuracy and efficiency of the experiment.

[0088] It is worth mentioning that the constant temperature tank body 21, the lifting tank 25 and the enclosure 27 all adopt a circular structure. This circular structure shows excellent performance in diversion and diversion, ensuring the uniform distribution of water flow in all directions, and further improving the accuracy of the experiment.

[0089] Constant temperature circulating water bath plays an important role in experimental research. It can provide a stable temperature environment, which is crucial for many chemical reactions and biological experiments. In the experiment, the constant temperature circulating water bath can accurately maintain the required temperature range to ensure the reliability and repeatability of the experimental results.

[0090] In chemical reactions, temperature stability has a significant impact on reaction rate and selectivity. Constant temperature circulating water baths can provide a constant temperature environment to help researchers control the progress of reactions, optimize reaction conditions, and improve experimental repeatability and accuracy.

[0091] In biological experiments, temperature has an important impact on processes such as cell growth, enzyme activity, and DNA amplification. The constant temperature water bath can be used to control and maintain the temperature of the sample, ensure the consistency of experimental conditions, and improve the repeatability and reliability of the experiment. However, after actual research, we found that the constant temperature water temperature in the constant temperature bath 2 has little effect on the cells to be tested in the cell bath 3, and does not seem to have a substantial impact on the experimental results. This discovery triggered our in-depth exploration of this phenomenon.

[0092] In order to study this phenomenon in depth, we need to quickly change the temperature of the constant temperature water. By setting the enclosure 27, we can not only separate the water bath space, but also significantly improve the stability of the inner water bath space, ensuring the consistency and stability of the experimental conditions when quickly changing the constant temperature water.

[0093] Specifically, an attachment bin 28 is fixed at the bottom of the thermostatic tank 21, and a lifting rod is fixed in the attachment bin 28 for lifting and lowering the enclosure 27;

[0094] The lifting rod is configured such that when the temperature of the thermostatic bath 2 is adjusted quickly, the lifting rod drives the enclosure 27 to descend so that the bottom of the enclosure 27 contacts the inner lower surface of the thermostatic bath body 21. At this time, the external thermostatic water circulation device extracts all the water in the external water bath space, and then injects water of the first temperature into the external water bath space. At this time, the lifting rod drives the enclosure 27 to rise slowly.

[0095] The enclosure 27 is made of heat-conducting material.

[0096] During the implementation, when the temperature of the thermostatic bath 2 needs to be adjusted quickly, the lifting rod drives the enclosure 27 to descend, so that its bottom contacts the inner lower surface of the thermostatic bath body 21. This action clearly separates the inner space of the thermostatic bath body 21 into an outer water bath space and an inner water bath space. This separation design avoids the impact of the outer water bath space on the inner water bath space, ensuring stability and accuracy during the temperature adjustment process.

[0097] Subsequently, the external constant temperature water circulation device extracts all the water in the outer water bath space. Subsequently, water at the first temperature is injected into the space. At this time, the lifting rod moves again, driving the enclosure 27 to slowly rise. This step realizes the rapid temperature adjustment of the constant temperature bath 2 while maintaining the stability of the inner water bath space.

[0098] It is worth mentioning that although the water in the inner water bath space is not replaced quickly, due to its relatively small capacity, the impact on the overall temperature is minimal. In the subsequent process, the water in the inner water bath space can be slowly replaced without significantly affecting the experimental results. This design concept fully reflects the flexibility and convenience of this system in experimental operation, while also ensuring the accuracy and reliability of the experimental results.

[0099] As a preferred embodiment, when the cell bath 3 is carried by the positioning cylinder 22 in the constant temperature bath 2, there is a first distance between the bottom of the cell bath 3 and the upper surface of the lifting groove 25, and the first distance is less than 8 mm.

[0100] As a preferred embodiment, the liquid level in the constant temperature bath 2 is lower than the lowest position of the upper surface of the enclosure 27 and higher than the bottom of the cell bath 3 .

[0101] In an embodiment of the present invention, a method for heat preservation of a constant temperature bath 2 is also provided, which comprises the following steps:

[0102] Step 1: Adjust the height of the enclosing plate 27 so that there is a gap between the lower surface of the enclosing plate 27 and the inner lower surface of the thermostatic bath 21, and the liquid level in the thermostatic bath 2 is lower than the lowest position of the upper surface of the enclosing plate 27 and higher than the bottom of the cell bath 3;

[0103] Step 2: When the cell bath 3 is carried by the positioning cylinder 22 in the constant temperature bath 2, there is a first distance between the bottom of the cell bath 3 and the upper surface of the lifting groove 25, and the first distance is less than 8 mm.

[0104] Step 3: Circulate constant temperature water through external constant temperature water circulation equipment.

[0105] In an embodiment of the present invention, a method for adjusting the temperature of a constant temperature bath 2 is also provided, which comprises the following steps:

[0106] Step 1: operate the lifting rod to drive the enclosure 27 to descend, so that its bottom contacts the inner lower surface of the thermostatic tank body 21, thereby clearly dividing the inner space of the thermostatic tank body 21 into an outer water bath space and an inner water bath space.

[0107] Step 2: Use external constant temperature water circulation equipment to extract all the water in the external water bath space.

[0108] Step 3: After the constant temperature water in the outer water bath space is completely pumped out, constant temperature water of the first temperature is injected into the space.

[0109] Step 4: The lifting rod drives the enclosure 27 to rise slowly, thereby realizing rapid temperature adjustment of the constant temperature bath 2 while maintaining the stability of the inner water bath space.

[0110] It is worth mentioning that although the water in the inner water bath space is not replaced quickly, due to its relatively small capacity, the impact on the overall temperature is minimal. In the subsequent process, the water in the inner water bath space can be slowly replaced without significantly affecting the experimental results. This design concept fully reflects the flexibility and convenience of this system in experimental operation, while also ensuring the accuracy and reliability of the experimental results.

[0111] What is described above is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A constant temperature patch clamp cell observation and recording system for cell experiments, characterized in that: include: A microscope (1) is placed on an anti-vibration table, the outside of which is provided with a shielding net; A constant temperature bath (2), which is placed on the microscope (1) and is used to provide a constant temperature environment; A cell bath (3), which is placed in the middle of the constant temperature bath (2) and is used to carry cells to be detected; A microelectrode controller (4), which is disposed on one side of the microscope (1) and is used to contact the cells to be detected and provide a clamping voltage; and A drug delivery system (5) for providing a drug solution environment for the cells to be tested in the cell bath (3); Wherein, the constant temperature bath (2) comprises: The thermostatic tank body (21) is made of a transparent material and has a middle portion that is concave upward to form a lifting groove (25); at least two symmetrically arranged positioning cylinders (22), which are fixed to the inner side of the thermostatic tank body (21) and are used to support the cell bath (3); and A liquid inlet (23) and a liquid outlet (24) connected to the side of the thermostatic tank (21), wherein the liquid inlet (23) and the liquid outlet (24) are symmetrically arranged and connected to an external thermostatic water circulation device; A water bath space is formed between the lifting tank (25) and the inner wall of the constant temperature tank body (21), and a baffle (27) is provided in the middle of the water bath space, and the baffle (27) can separate the water bath space into an outer water bath space and an inner water bath space; An attachment bin (28) is fixed at the bottom of the thermostatic tank (21), and a lifting rod is fixed in the attachment bin (28) for lifting and lowering the enclosure (27); The lifting rod is configured such that when the temperature of the thermostatic bath (2) is adjusted quickly, the lifting rod drives the enclosure (27) to descend so that the bottom of the enclosure (27) contacts the inner lower surface of the thermostatic bath body (21). At this time, the external thermostatic water circulation device extracts all the water in the external water bath space, and then injects water at a first temperature into the external water bath space. At this time, the lifting rod drives the enclosure (27) to slowly rise. The enclosure plate (27) is made of a heat-conducting material.

2. A constant temperature patch clamp cell observation and recording system for cell experiments according to claim 1, characterized in that: The top of the lifting groove (25) has an arc angle (26).

3. A constant temperature patch clamp cell observation and recording system for cell experiments according to claim 1, characterized in that: The constant temperature tank body (21), the lifting tank (25) and the enclosure (27) are all circular structures.

4. A constant temperature patch clamp cell observation and recording system for cell experiments according to claim 1, characterized in that: When the cell bath (3) is carried by the positioning cylinder (22) in the constant temperature bath (2), there is a first distance between the bottom of the cell bath (3) and the upper surface of the lifting groove (25), and the first distance is less than 8 mm.

5. A constant temperature patch clamp cell observation and recording system for cell experiments according to claim 1, characterized in that: The liquid level in the constant temperature bath (2) is lower than the lowest position of the upper surface of the enclosure (27) and higher than the bottom of the cell bath (3).

6. A constant temperature patch clamp cell observation and recording system for cell experiments according to claim 1, characterized in that: The cell bath (3) comprises: A cell tank body (31) having an outer diameter smaller than the outer diameter of the lifting tank (25); A clamping column (33) capable of being clamped into the positioning cylinder (22); and An extension rod (32) is connected between the clamping column (33) and the cell tank (31).

7. A constant temperature patch clamp cell observation and recording system for cell experiments according to claim 6, characterized in that: The positioning cylinder (22) comprises: A column groove (221) is provided on the upper surface of the positioning cylinder (22) and is used for clamping the clamping column (33); An elastic tube (222) attached to the inner surface of the column groove (221) and used for locking the clamping column (33); A plurality of expansion grooves are provided on the lower surface of the positioning cylinder (22) and are connected to the column groove (221); and an expansion column (224) embedded in the expansion groove, wherein the expansion column (224) can expand by absorbing water; A locking pad (223) is provided at the connection point between the expansion groove and the column groove (221), and the locking pad (223) is made of elastic and water-impermeable material.

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