A synergistic compound composition containing nicotine insecticide and its application

Abstract

The present invention belongs to the field of pesticides and relates to a synergistic compound composition containing two active components. The composition contains two active components A and B, wherein component A is selected from the following structural compounds and component B is selected from nicotine insecticides; the weight ratio of the two active components A and B is 1:50 to 50:1; the composition of the present invention has an obvious synergistic effect and can be used to control a variety of pests.

Description

Technical Field

[0001] The invention belongs to the field of pesticides and relates to a synergistic compound composition containing nicotine insecticides and application thereof. Background Art

[0002] In the actual process of agricultural production, the application of chemical agents is one of the most effective means of preventing and controlling plant diseases and insect pests. However, the most common problem caused by chemical pest control is insect resistance. At the same time, the long-term continuous high-dose application of a single chemical agent is likely to cause a series of problems such as agent residues and environmental pollution. Reasonable chemical agent compounding or mixing has the positive characteristics of expanding the control spectrum, improving the control effect, extending the appropriate period of application, reducing the amount of medicine used, reducing drug damage, reducing residues, and delaying the occurrence and development of pest resistance and drug resistance. Chemical agent compounding or mixing is one of the most effective ways to solve the above problems.

[0003] Chemical pesticides, as an important guarantee for stable and abundant crop yields, play an irreplaceable role in pest control. Neonicotinoid insecticides are an important type of chemical pesticide in the world. They selectively control the nicotinic acetylcholinesterase receptors (nAChRs) in the insect nervous system, paralyzing the central nervous system and causing the death of insects. They have excellent control effects on homoptera, coleoptera, lepidoptera and even resistant target pests. Due to their unique mechanism of action, nicotinoid insecticides have no cross-resistance with conventional pesticides, are highly efficient, broad-spectrum, and have good root systemic, contact and stomach poisoning effects, and are safe for the environment. They are one of the agents that can replace highly toxic and high-residue organophosphorus, carbamate and organochlorine pesticides.

[0004] Imidacloprid is an early-used neonicotinoid insecticide. For a long time, it has been widely used to control planthoppers, aphids, whiteflies, etc. However, since 2005, many pests have developed a high degree of resistance to imidacloprid. Acetamiprid, thiamethoxam, thiamethoxam, nitenpyram, dinotefuran and clothianidin are neonicotinoid insecticides with excellent pest control effects. With the continuous and large-scale investment in neonicotinoid pesticides in the agricultural ecological environment, a series of scientific issues such as target resistance, ecological risks, and human health have also emerged. The combination of different pesticides can not only delay the target resistance of a single pesticide and improve the activity of pesticides, but also reduce the amount of pesticides used and reduce the risk of environmental exposure, providing broad prospects for the sustainable application of pesticides.

[0005] Chinese invention patent CN112457288 discloses a piperic acid derivative and its application, wherein compound I-72 has contact and stomach poison activity and good conduction activity, and can be used to control a variety of Lepidoptera, Hemiptera, Thysanoptera, Coleoptera and other pests. The compound has the following structural formula:

[0006]

[0007] The combination of different ingredients may produce some unexpected technical effects and is also an effective method for preventing and controlling resistant pests. However, there is no report on the combination of compound I-72 and nicotine insecticides. Summary of the invention

[0008] The object of the present invention is to provide a synergistic compound composition containing nicotine insecticides and application thereof.

[0009] To achieve the above purpose, the technical solution adopted by the present invention is:

[0010] A synergistic compound composition containing a nicotine insecticide, the composition comprises two active components A and B, component A is selected from the following structural compound (i.e., compound I-72), and component B is selected from nicotine insecticides; the weight ratio of the two active components of components A and B is 1:50 to 50:1; wherein the compound structure of component A is as follows:

[0011]

[0012] The component B is selected from the group consisting of B1 acetamiprid, B2 thiamethoxam, B3 clothianidin, B4 thiacloprid, B5 imidacloprid, B6 nitenpyram, B7 dinotefuran, B8 cycloxaprid, B9 guadipyr, B10 flupyradifurone, B11 sulfoxaflor, B12 imidaclothiz, B13 sulfoneworm, B14 paichongding, B15 pyridinsulfuron, B16 nicotine, B17 anabasine, B18 nornicotine, and B19 flupyrimin;

[0013] Preferably, in the composition, component A is selected from the above-mentioned structural compounds; component B is selected from B1 acetamiprid, B2 thiamethoxam, B3 clothianidin, B4 thiacloprid, B5 imidacloprid, B6 nitenpyram, B7 dinotefuran, B8 cycloxaprid, B9 guadipyr, B10 flupyradifurone, B11 sulfoxaflor, B12 imidaclothiz, B13 sulfoneworm, B14 paichongding, B15 pyridinium, B16 nicotine, B17 anabasine, B18 nornicotine, B19 flupyrimin; the weight ratio of the two active components, component A and component B, is 1:20 to 20:1.

[0014] Further preferably, component A in the composition is selected from the above-mentioned structural compounds; component B is selected from B1 acetamiprid, B3 clothianidin, B5 imidacloprid, B2 thiamethoxam, B6 nitenpyram, B7 dinotefuran, B4 thiacloprid, B11 sulfoxaflor, and B16 nicotine; the weight ratio of the two active components of components A and B is 1:10 to 10:1.

[0015] The application of the composition is used for preparing insecticide drugs for preventing and controlling pests on fruit trees, vegetables, ornamental plants, tea, cotton, cereal crops, shrubs, broad-leaved trees and evergreen trees.

[0016] Use of the composition for preventing or controlling pests of Lepidoptera, Hemiptera, Homoptera, Coleoptera, Diptera, Thysanoptera, Acarina, and their larvae and eggs

[0017] The insecticide composition of the present invention has a significant synergistic effect in controlling pests on fruit trees, vegetables, ornamental plants, tea, cotton, cereal crops, shrubs, broad-leaved trees, and evergreen trees. Indoor biological activity assay results show that mixing compound I-72 with nicotine insecticides can improve the control effect, reduce the dosage, and expand the insecticide spectrum. For example, when compound I-72 is mixed with imidacloprid, acetamiprid, thiamethoxam, clothianidin, nitenpyram, dinotefuran, thiacloprid, sulfoxaflor, etc. in a ratio of 1:10 to 10:1, the activity against targets such as peach aphid, cotton aphid, thick plum nest moth, brown planthopper, and whitefly can be greatly improved; through a large number of experiments, it is found that after compound I-72 is mixed with nicotine insecticides, there is a significant synergistic trend with the increase of the amount of the compound, which is conducive to the application of the compound in the field. Therefore, the two active component compositions of the present invention can be used for the preparation of insecticide drugs.

[0018] The synergistic compound composition of the present invention can be used to control pests and underground pests on fruit trees, vegetables, ornamental plants, tea, cotton, cereal crops, shrubs, broad-leaved trees, evergreen trees, etc.; it can be particularly used to control piercing-sucking mouthparts pests on a variety of crops, such as planthoppers, whiteflies, aphids, thrips, leafhoppers, stink bugs, as well as striped stem borers, corn borers, diamondback moths, armyworms, leaf miners, cotton bollworms, beet armyworms, etc.

[0019] In particular, the synergistic composite composition of the present invention is particularly useful for controlling Hemiptera pests, a non-exhaustive list of which includes, but is not limited to, Acrosternum hilare, green stink bug, Blissus leucopterus, chinch bug, Calocoris norvegicus, Cimex hemipterus, tropical bed bug, Cimex lectularius, bed bug, Dagbertus fasciatus, Dichelops furcatus, Dysdercus suturellus, cotton bug, Edessa meditabunda, Eurygaster maura, cereal bug, Euschistus neotropicalis heros), Euschistus servus, Brown stink bug, Helopeltis antonii, Helopeltis theivora, Tea horn stink bug, Lagynotomus spp., stink bugs, Leptocorisa oratorius, Leptocorisa varicornis, Lygus spp., Lygus, Lygus hesperus, Western grass stink bug, Maconellicoccus hirsutus, Neurocolpus longirostris, Nezara viridula, Southern green stink bug, Phytocoris spp., Lygus, California plant stink bug californicus), Phytocoris relativus, Piezodorus guildingi, Poecilocapsus lineatus, Four-lined stink bug, Psallus vaccinicola, Pseudacysta perseae, Scaptocoris castanea, and Triatoma spp., bloodsucking conenose bugs / kissing bugs.

[0020] In particular, the synergistic composite composition of the present invention is particularly useful for controlling homopteran pests (aphids, scale insects, whiteflies, planthoppers, leafhoppers), a non-exhaustive list of which includes, but is not limited to, pea aphids (Acrythosiphon pisum), pea aphids, Adelges spp., aphids, cabbage whitefly (Aleurodes proletella), cabbage whitefly, spiral whitefly (Aleurodicus disperses), soft hair whitefly (Aleurothrixus floccosus), cotton whitefly, Aluacaspis spp., Amrasca bigutella bigutella, Aphrophora spp., leafhoppers, Aonidiella aurantii, Aphis spp., aphids of Aphis spp., cotton aphids (Aphis gossypii), cotton aphids, apple aphids (Aphis pomi), apple aphid, Aulacorthum solani, foxglove aphid, Bemisia spp., whitefly, Bemisia argentifolii, Bemisia tabaci, sweet potato whitefly, Brachycolus noxius, Russian aphid, Brachycorynella asparagi, asparagus aphid, Brevennia rehi, Brevicoryne brassicae, cabbage aphid, Ceroplastes spp. scale insects, Ceroplastes rubens, red wax scale insects, Chionaspis spp. scale insects, Chrysomphalus spp.), scale insects of the genus Coccus, Dysaphis plantaginea, pink apple aphid, leafhoppers of the genus Empoasca, woolly apple aphids Eriosoma lanigerum, woolly apple aphids, Icerya purchasi, woolly scale insects, Idioscopus nitidulus, mango leafhoppers, Laodelphax striatellus, brown planthoppers, Lepidosaphes, Macrosiphum.), potato aphid (Macrosiphum euphorbiae), potato aphid, wheat aphid (Macrosiphum granarium), English wheat aphid, rose aphid (Macrosiphum rosae), rose aphid, aster leafhopper (Macrosteles quadrilineatus), aster leafhopper, raspberry foamhopper (Mahanarva frimbiolata), wheat aphid (Metopolophium dirhodum), rose wheat aphid, long-horned bug (Mictis longicornis), peach aphid (Myzus persicae), green peach aphid, leafhoppers of the genus Nephotettix (Nephotettix spp.), black-tailed leafhopper (Nephotettix cinctipes), green leafhopper, green leafhopper, rice brown planthopper (Nilaparvata lugens), brown planthopper, bran shield scale (Parlatoria pergandii), bran scale, black scale scale (Parlatoria ziziphi), ebony scale, corn planthopper (Peregrinus maidis), corn flower-winged planthopper, Philaenus spp.'s scale, grape phylloxera (Phylloxera vitifoliae), grape phylloxera, spruce scale (Physokermes piceae), spruce round scale, planococcus spp., mealybugs, mealybugs of Pseudococcus spp., pineapple mealybug (Pseudococcus brevipes), pineapple mealybug, pear round scale (Quadraspidiotus perniciosus), St. Joseph's scale, Rhapalosiphum spp.), corn leaf aphids (Rhapalosiphummaida), corn aphids, grain aphids (Rhapalosiphum padi), grain aphids, scale insects of the genus Saissetia spp., olive black helmet scale insects (Saissetia oleae), black scale insects, Schizaphis graminum, green aphids, wheat long-tube aphids (Sitobion avenae), English wheat aphids, white-backed rice hoppers (Sogatella furcifera), white-backed planthoppers, aphids of the genus Therioaphis spp., scale insects of the genus Toumeyella spp., aphids of the genus Toxoptera spp., whiteflies (Trialeurodes spp.), greenhouse whitefly (Trialeurodes vaporariorum), greenhouse whitefly, green hemp whitefly (Trialeurodes abutiloneus), winged whitefly, scale insects of the genus Unaspis spp., Unaspis yanonensis, arrowhead scale insects, and Zulia entreriana.

[0021] In particular, the synergistic combination composition of the present invention is particularly useful for controlling Thysanoptera pests (thrips), a non-exhaustive list of which includes, but is not limited to, Frankliniella fusca, Tobacco thrips, Frankliniella occidentalis, Western flower thrips, Frankliniella shultzei, Frankliniella williamsi, Corn thrips, Heliothrips haemorrhaidalis, Greenhouse thrips, Riphiphorothrips cruentatus, Scirtothrips spp., Scirtothrips citri, Citrus thrips, Scirtothrips dorsalis, Scirtothrips dorsalis, Taeniothrips rhopalantennalis, and Thrips spp.

[0022] In particular, the synergistic composite composition of the present invention is particularly useful for controlling coleopteran (beetle) pests, a non-exhaustive list of which includes, but is not limited to: Acanthoscelides spp., weevils, Acanthoscelides obtectus, soybean weevils, Agrilus planipennis, ash borers, Agriotes spp., wireworms, Anoplophora glabripennis, Asian longhorn beetles, Anthonomus spp., weevils, Anthonomus grandis, boll weevils, Aphidius spp., Apion spp., weevils, Apogonia spp., grubs, Ataenius spretulus, turfgrass black beetle, beet cryptic beetle (Atomaria linearis), small beet beetle, melon beetle species (Aulacophora spp.), beet weevil (Bothynoderes punctiventris), beet root weevil, bean weevil species (Bruchus spp.), pea weevil (Bruchus pisorum), pea weevil, Cacoesia species, four-striped bean weevil (Callosobruchusmaculatus), southern cowpea weevil, yellow-spotted dew beetle (Carpophilus hemipteras), dried fruit beetle, beet tortoise beetle (Cassida vittata), Cerosterna species, Cerotoma species, leaf beetle, bean leaf beetle (Cerotomatrifurcata), bean leaf beetle, tortoise beetle species (Ceutorhynchus spp.), cabbage weevil (Ceutorhynchus assimilis), cabbage heart weevil, citrus weevil (Ceutorhynchus napi), cabbage weevil, Chaetocnema spp., Colaspis spp.), soil beetles, Conoderus scalaris, Conoderus stigmosus, Conotrachelus nenuphar, plum cone weevil, Cotinus nitidis, green flower beetle, Crioceris asparagi, asparagus leaf beetle, Cryptolestes ferrugineus, rusty flat flour beetle, Cryptolestes pusillus, flat flour beetle, Turkish flat flour beetle (Cryptolestesturcicus), Turkish flour beetle, Ctenicera spp., Curculio spp., Cyclocephala spp., Cylindrocpturus adspersus, sunflower stem weevil, Deporaus marginatus), mango leaf cutter weevil, Dermesteslardarius, ham weevil, Dermestes maculates, hidden beetle, Diabrotica spp. chrysolemid, Epilachna varivestis, Mexican bean beetle, Faustinus cubae, pale bark weevil, aggressive weevil, Hypera spp., Hypera postica, clover weevil, Hyperdoes spp., Argentine stem weevil, Hypothenemus hampei, coffee berry beetle, Ips spp., engraver beetle, Lasioderma serricorne), tobacco beetle, potato beetle (Leptinotarsa ​​decemlineata), Colorado potato beetle, Liogenys fuscus, Liogenys suturalis, Lissorhoptrus oryzophilus, rice water weevil, Lyctus spp., wood beetle, powder beetle, Maecolaspis joliveti, Megascelis spp., corn beetle (Melanotus communis), cauliflower beetle (Meligethes spp.), Meligethes aeneus, Flower beetles, Melolontha melolontha, Common European beetle, Oberea brevis, Oberea linearis, Oryctes rhinoceros, Date palm beetle, Oryzaephilus mercator, Market saw-toothed grain beetle, Oryzaephilus surinamensis, Saw-toothed grain beetle, Otiorhynchus spp., Oulema melanopus, Grain leaf beetle, Oulema oryzae, Pantomorus spp., Phyllophaga spp., May / June beetle, June beetle cuyabana), Phyllotreta spp., Phyllotretavittuta fabr., Yellow curvy flea beetle, Yellow straight flea beetle, Yellow narrow flea beetle, Yellow wide flea beetle, Leaf beetle, Phynchites spp., Popillia japonica, Japanese beetle, Prostephanus truncates, Rhizopertha dominica, Rhizopertha dominica, Rhizotrogus spp., European beetle, Rhynchophorus spp., Scolytus spp., Wood beetle), Shenophorus spp., Sitonalineatus, Pea leaf weevil, Sitophilus spp. spp.), cereal weevils, grain weevils (Sitophilusgranaries), grain worms, rice weevils (Sitophilus oryzae), rice weevils, small round skin beetles (Stegobiumpaniceum), stealing beetles, Tribolium spp., flour beetles, red flour beetles (Triboliumcastaneum), red flour beetles, mixed flour beetles (Triboliumconfusum), mixed flour beetles, spotted skin beetles (Trogodermavariabile), warehouse beetles and greedy carabids (Zabrustenebioides).

[0023] In particular, the synergistic compound composition of the present invention can be used to control pests such as two-spotted spider mites, cinnabar spider mites, citrus leafminers, citrus scale insects, fruit leafminers, fruit borers, soybean borers, golden moths, tea geometrids, tea caterpillars, tea green leafhoppers, pear psyllids, rice planthoppers, greenhouse whiteflies, American leafminers, yellow-striped flea beetles, striped stem borers, yellow stem borers, corn borers, bean pod borers, melon borers, rice water weevils, cotton bollworms, pink bollworms, fruit aphids, soybean aphids, vegetable aphids, wheat aphids, cotton aphids, diamondback moths, armyworms, cabbage worms, tobacco budworms, and longhorn beetles.

[0024] The synergistic compound composition of the present invention can be applied at an effective dose to the pests / mites to be controlled and / or their habitats. Therefore, the synergistic compound composition of the present invention provides a method for controlling or preventing plant pests.

[0025] The active ingredients in the synergistic compound composition of the present invention can be applied to pests, plants and their habitats simultaneously (for example, as a preformulated mixture or tank mix) or in a suitable sequence.

[0026] Advantages of the present invention: The binary composition obtained by compounding compound I-72 and nicotine insecticide in a suitable ratio has a good synergistic effect, thereby improving the prevention effect, reducing the dosage, and expanding the insecticide spectrum. Specifically: 1. The compounded two active components A and B have different mechanisms of action and can delay the development of insecticide resistance. 2. The insecticide spectrum is expanded and can be used to prevent and control various pests on various crops such as fruit trees, vegetables, ornamental plants, tea, cotton, etc.; 3. The amount of pesticide used is reduced, the amount of pesticide residues on crops is reduced, and the friendliness to the environment is enhanced. 4. The two active components A and B are compounded within a suitable ratio range to improve the control effect of pests such as rice brown planthoppers, peach aphids, and cotton aphids. DETAILED DESCRIPTION

[0027] The following examples are used to explain the present invention in detail, so as to facilitate further understanding of the present invention. The active component A is selected from compound I-72 (prepared according to the description in the patent document CN112457288, with a content of 97%); the active component B and other raw materials are all commercially available products, and each component is measured by weight percentage.

[0028] Example 1: Indoor combined toxicity test of compound I-72 and clothianidin against green peach aphid

[0029] Test target: Green peach aphid [Myzus persicae (Sulzer)] nymphs, a susceptible strain reared indoors.

[0030] Preparation of liquid medicine: According to different test requirements, accurately weigh the test samples respectively with an electronic analytical balance. The technical material is dissolved in acetone and then diluted with 0.1% Tween 80 water into a series of liquid medicines with certain concentration gradients according to the test design dosage.

[0031] Test method: First, select cabbage leaves with neat and consistent green peach aphids, with about 30 - 40 insects per leaf. Then, spray evenly in the order from low dose to high dose according to the test design, 1.5 mL per leaf, and repeat each treatment 3 times. Additionally, set a blank control.

[0032] Place the treated test materials in the observation chamber, where the temperature, humidity, and light in the observation chamber can be adjusted as needed. After 48 hours, investigate the number of dead and live insects, calculate the corrected mortality rate using the Abbott formula, and conduct statistical analysis using the DPS data processing software to obtain the toxicity regression equations, LC 50 values, and 95% confidence limits of each tested single agent and each different ratio mixture. Then, use the Sun Yun - pei method to calculate the co - toxicity coefficient of each ratio to evaluate the mixing effect. The test results are shown in Table 1.

[0033] The co - toxicity coefficient (CTC value) of the mixture is calculated according to the following formula:

[0034]

[0035] In the formula: ATI - Measured toxicity index of the mixture; S - LC 50 of the standard agent; M - LC 50 of the mixture.

[0036] TTI = TIA×PA + TIB×PB

[0037] In the formula: TTI - Theoretical toxicity index of the mixture; TIA - Toxicity index of agent A; PA - Percentage content of agent A in the mixture; TIB - Toxicity index of agent B; PB - Percentage content of agent B in the mixture.

[0038]

[0039] In the formula: CTC - Co - toxicity coefficient; ATI - Measured toxicity index of the mixture; TTI - Theoretical toxicity index of the mixture.

[0040] The co - toxicity coefficient (CTC) of the compound mixture: CTC≥120 indicates synergistic effect; 80 < CTC < 120 indicates additive effect; CTC≤80 indicates antagonistic effect.

[0041] Table 1 Results of indoor joint toxicity determination of the mixture of Compound I - 72 and clothianidin against green peach aphids

[0042]

[0043]

[0044] Example 2: Indoor combined toxicity test of compound I-72 and acetamiprid against yellow striped flea beetle

[0045] Test target: Adult yellow-striped flea beetle (Phyllotreta vittuta Fabr.).

[0046] Preparation of drug solution: According to different test needs, use an electronic analytical balance to accurately weigh the test samples, dissolve the original drug in acetone, and then use 0.1% Tween 80 water to dilute it into a series of drug solutions with a certain concentration gradient according to the designed experimental dosage.

[0047] Experimental method: First, select neat and consistent potted cabbage seedlings, and then spray them evenly in order from low dose to high dose according to the experimental design. After drying in the shade, transfer the treated cabbage seedlings to the adult flea beetles and cover them with glass covers, about 30-40 heads per plant. Each treatment is repeated 3 times, and a blank control is set up.

[0048] The treated test materials were placed in an observation room, where the temperature, humidity, and light intensity could be adjusted as needed. After 72 hours, the number of dead and live insects was investigated, and the corrected mortality rate was calculated using the Abbott formula. The DPS data processing software was used for statistical analysis to determine the toxicity regression equations, LC values, and the toxicity of each single agent and each different ratio of the mixture. 50 The value and 95% confidence limit were calculated, and then the co-toxicity coefficient of each ratio was calculated by Sun Yunpei (Sun yp) method to evaluate the mixing effect (same as Example 1). The test results are shown in Table 2.

[0049] Table 2 Results of indoor combined toxicity test of compound I-72 and acetamiprid against yellow-striped flea beetle

[0050]

[0051] Example 3: Indoor joint toxicity test of compound I-72 and thiamethoxam against thrush moth

[0052] Test target: 3rd instar larvae of the plum moth Yponomeuta evonymallus (Linnaeus).

[0053] Preparation of drug solution: According to different test needs, use an electronic analytical balance to accurately weigh the test samples, dissolve the original drug in acetone, and then use 0.1% Tween 80 water to dilute it into a series of drug solutions with a certain concentration gradient according to the designed experimental dosage.

[0054] Experimental method: First, select Sedum leaves, and immerse them in the prepared solution for 10 seconds in the order from low dose to high dose according to the experimental design. After natural drying, place them in a culture dish with a diameter of 9 cm and filter paper, and inoculate neatly arranged healthy test insects. Each treatment has 10 heads, and each treatment is repeated 3 times. A blank control is also set up.

[0055] The treated test materials were placed in an observation room, where the temperature, humidity, and light intensity could be adjusted as needed. After 72 hours, the number of dead and live insects was investigated, and the corrected mortality rate was calculated using the Abbott formula. The DPS data processing software was used for statistical analysis to determine the toxicity regression equations, LC values, and the toxicity of each single agent and each different ratio of the mixture. 50 The value and 95% confidence limit were calculated, and then the co-toxicity coefficient of each ratio was calculated by Sun Yunpei (Sun yp) method to evaluate the mixing effect (same as Example 1). The test results are shown in Table 3.

[0056] Table 3 Results of indoor combined toxicity test of compound I-72 and thiamethoxam against thrush moth

[0057]

[0058] Example 4: Indoor combined toxicity test of compound I-72 and nitenpyram on cotton aphid

[0059] Test target: cotton aphid (Aphis gossypii Glover) nymphs, a susceptible strain reared indoors.

[0060] Preparation of drug solution: According to different test needs, use an electronic analytical balance to accurately weigh the test samples, dissolve the original drug in acetone, and then use 0.1% Tween 80 water to dilute it into a series of drug solutions with a certain concentration gradient according to the designed experimental dosage.

[0061] Test method: First, select cotton leaves with neat and consistent cotton aphids, with each leaf carrying about 30-40 insects. Then, spray evenly from low dose to high dose according to the test design, 1.5 mL per leaf, repeat each treatment 3 times, and set up a blank control.

[0062] The treated test materials were placed in an observation room, where the temperature, humidity, and light intensity could be adjusted as needed. After 48 hours, the number of dead and live insects was investigated, and the corrected mortality rate was calculated using the Abbott formula. The DPS data processing software was used for statistical analysis to determine the toxicity regression equations, LC values, and the toxicity of each single agent and each different ratio of the mixture. 50 The value and 95% confidence limit were calculated, and then the co-toxicity coefficient of each ratio was calculated by Sun Yunpei (Sun yp) method to evaluate the mixing effect (same as Example 1). The test results are shown in Table 4.

[0063] Table 4 Results of indoor combined toxicity test of compound I-72 and nitenpyram against cotton aphid

[0064]

[0065]

[0066] Example 5: Indoor combined toxicity test of compound I-72 and dinotefuran against western flower thrips

[0067] Test target: Nymphs of western flower thrips (Frankliniella occidentalis).

[0068] Preparation of drug solution: According to different test needs, use an electronic analytical balance to accurately weigh the test samples, dissolve the original drug in acetone, and then use 0.1% Tween 80 water to dilute it into a series of drug solutions with a certain concentration gradient according to the designed experimental dosage.

[0069] Experimental method: First, select bean leaves, and immerse them in the prepared solution for 10 seconds in the order from low dose to high dose according to the experimental design. After natural drying, place them in a culture dish with a diameter of 9 cm and filter paper, and inoculate neatly packed healthy test insects, about 20-30 heads per treatment, and repeat 3 times for each treatment, with a blank control.

[0070] The treated test materials were placed in an observation room, where the temperature, humidity, and light intensity could be adjusted as needed. After 48 hours, the number of dead and live insects was investigated, and the corrected mortality rate was calculated using the Abbott formula. The DPS data processing software was used for statistical analysis to determine the toxicity regression equations, LC values, and the toxicity of each single agent and each different ratio of the mixture. 50 The value and 95% confidence limit were calculated, and then the co-toxicity coefficient of each ratio was calculated by Sun Yunpei (Sun yp) method to evaluate the mixing effect (same as Example 1). The test results are shown in Table 5.

[0071] Table 5 Results of indoor combined toxicity test of compound I-72 and dinotefuran against western flower thrips

[0072]

[0073] Example 6: Indoor joint toxicity test of compound I-72 and sulfoxaflor against broad bean aphids

[0074] Test target: Bean aphid (Aphis craccivora) nymphs, a susceptible strain reared indoors.

[0075] Preparation of drug solution: According to different test needs, use an electronic analytical balance to accurately weigh the test samples, dissolve the original drug in acetone, and then use 0.1% Tween 80 water to dilute it into a series of drug solutions with a certain concentration gradient according to the designed experimental dosage.

[0076] Experimental method: First, select broad bean seedlings with neat and consistent broad bean aphids, with an insect load of about 30-40 aphids per plant. Then, spray evenly from low dose to high dose according to the experimental design, 1.5 mL per leaf, repeat each treatment 3 times, and set up a blank control.

[0077] The treated test materials were placed in an observation room, where the temperature, humidity, and light intensity could be adjusted as needed. After 48 hours, the number of dead and live insects was investigated, and the corrected mortality rate was calculated using the Abbott formula. The DPS data processing software was used for statistical analysis to determine the toxicity regression equations, LC values, and the toxicity of each single agent and each different ratio of the mixture. 50 The value and 95% confidence limit were calculated, and then the co-toxicity coefficient of each ratio was calculated by Sun Yunpei (Sun yp) method to evaluate the mixing effect (same as Example 1). The test results are shown in Table 6.

[0078] Table 6 Results of indoor combined toxicity test of compound I-72 and sulfoxaflor against broad bean aphids

[0079]

[0080] Example 7: Indoor combined toxicity test of compound I-72 and imidacloprid against rice brown planthopper

[0081] Test target: Rice brown planthopper [Nilaparvata lugens (Stal)] nymphs, a susceptible strain reared indoors.

[0082] Preparation of drug solution: According to different test needs, use an electronic analytical balance to accurately weigh the test samples, dissolve the original drug in acetone, and then use 0.1% Tween 80 water to dilute it into a series of drug solutions with a certain concentration gradient according to the designed experimental dosage.

[0083] Test method: The pot seedling spray method was used to determine the activity against rice brown planthopper nymphs. First, rice seedlings were planted in a culture pot and sprayed evenly in the order from low dose to high dose according to the experimental design, 2 mL per plant, and then rice brown planthopper nymphs of the same size were inoculated on the rice seedlings. Each treatment was repeated 3 times, and a blank control was set up.

[0084] The treated test materials were placed in an observation room, where the temperature, humidity, and light intensity could be adjusted as needed. After 72 hours, the number of dead and live insects was investigated, and the corrected mortality rate was calculated using the Abbott formula. The DPS data processing software was used for statistical analysis to determine the toxicity regression equations, LC values, and the toxicity of each single agent and each different ratio of the mixture. 50 The co-toxicity coefficient of each ratio was calculated by Sun Yunpei's method to evaluate the mixing effect (same as Example 1). The test results are shown in Table 7.

[0085] Table 7 Results of indoor combined toxicity test of compound I-72 and imidacloprid against rice brown planthopper

[0086]

[0087] Example 8: Indoor combined toxicity test of compound I-72 and thiacloprid on Bemisia tabaci nymphs

[0088] Test target: Bemisia tabaci nymphs, a susceptible strain reared indoors.

[0089] Preparation of drug solution: According to different test needs, use an electronic analytical balance to accurately weigh the test samples, dissolve the original drug in acetone, and then use 0.1% Tween 80 water to dilute it into a series of drug solutions with a certain concentration gradient according to the designed experimental dosage.

[0090] Experimental method: First, select cotton leaves with neat and consistent whitefly nymphs and make leaf discs. Each leaf disc carries about 20 insects. Then, spray them evenly in the order from low dose to high dose according to the experimental design, 1.5 mL per leaf, and repeat each treatment 3 times. Set up a blank control.

[0091] The treated test materials were placed in an observation room, where the temperature, humidity, and light intensity could be adjusted as needed. After 96 hours, the number of dead and live insects was investigated, and the corrected mortality rate was calculated using the Abbott formula. The DPS data processing software was used for statistical analysis to determine the toxicity regression equations, LC values, and the toxicity of each single agent and each different ratio of the mixture. 50 The value and 95% confidence limit were calculated, and then the co-toxicity coefficient of each ratio was calculated by Sun Yunpei (Sun yp) method to evaluate the mixing effect (same as Example 1). The test results are shown in Table 8.

[0092] Table 8 Results of indoor combined toxicity test of compound I-72 and thiacloprid on Bemisia tabaci nymphs

[0093]

[0094]

[0095] Example 9: Indoor joint toxicity test of compound I-72 and nicotine against spiraea aphid

[0096] Test target: Spiraea aphid (Aphis citricolavander Goot) nymph.

[0097] Preparation of drug solution: According to different test needs, use an electronic analytical balance to accurately weigh the test samples, dissolve the original drug in acetone, and then use 0.1% Tween 80 water to dilute it into a series of drug solutions with a certain concentration gradient according to the designed experimental dosage.

[0098] Test method: First, select apple leaves with neat and consistent spiraea aphids, with each leaf carrying about 30-40 insects. Then, spray evenly from low dose to high dose according to the test design, 1.5 mL per leaf, repeat each treatment 3 times, and set up a blank control.

[0099] The treated test materials were placed in an observation room, where the temperature, humidity, and light intensity could be adjusted as needed. After 48 hours, the number of dead and live insects was investigated, and the corrected mortality rate was calculated using the Abbott formula. The DPS data processing software was used for statistical analysis to determine the toxicity regression equations, LC values, and the toxicity of each single agent and each different ratio of the mixture. 50 The value and 95% confidence limit were calculated, and the co-toxicity coefficient of each ratio was calculated by Sun Yunpei (Sun yp) method to evaluate the mixing effect (same as Example 1). The test results are shown in Table 9.

[0100] Table 9 Results of indoor combined toxicity test of compound I-72 and nicotine against Spiraea aphid

[0101]

[0102] The description of the above embodiments is only used to help understand the method of the present invention and its core idea. It should be pointed out that for those of ordinary skill in the art, without departing from the principle of the present invention, the present invention can also be improved and modified in some ways, and these improvements and modifications also fall within the protection scope of the claims of the present invention. As can be seen from the above technical scheme, the synergistic composite composition of the present invention has the advantages of improving drug efficacy, expanding insecticide spectrum, reducing pesticide dosage, reducing the residual amount of pesticide on crops, alleviating pollution to the environment, being safe for humans and animals, having good environmental compatibility, and pests being difficult to develop drug resistance. The composition of the present invention can be applied to the prevention and treatment of various pests of various crops, and can be widely used in the field of agriculture.

Claims

1. A synergistic compound composition containing nicotine insecticides, characterized in that: The composition comprises two active components A and B, wherein component B is selected from B1 acetamiprid, B2 thiamethoxam, B3 clothianidin, B4 thiacloprid, B5 imidacloprid, B6 nitenpyram, B7 dinotefuran, B11 sulfoxaflor, and B16 nicotine; the weight ratio of the two active components A and B is 1:50 to 50:1; wherein the compound structure of component A is as follows: 。 2. The composition according to claim 1, characterized in that: The weight ratio of the two active components, component A and component B, is 1:20 to 20:

1.

3. The composition according to claim 1, characterized in that: The weight ratio of the two active components A and B is 1:10 to 10:

1.

4. Use of the composition according to claim 1, characterized in that: The composition is used for preparing insecticide drugs for preventing and controlling green peach aphids, yellow-striped flea beetles, plum moths, cotton aphids, western flower thrips, broad bean aphids, rice brown planthoppers, whiteflies and spiraea aphids on fruit trees, vegetables, ornamental plants, tea, cotton, cereal crops, shrubs, broad-leaved trees and evergreen trees.

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