Device and method for detecting microorganisms in traditional Chinese medicine

Abstract

The present application relates to the technical field of detection of microorganisms in traditional Chinese medicine, and provides a detection device and method for microorganisms in traditional Chinese medicine. The detection device for microorganisms in traditional Chinese medicine includes a chip body, a magnetic component and a peristaltic pump. The chip body is formed with a liquid inlet, a mixing pipe, a detection chamber and a liquid outlet which are connected in sequence, and the peristaltic pump is connected to the liquid outlet; wherein the mixing pipe includes a first pipe body, a second pipe body and a mixing structure located between the first pipe body and the second pipe body, and the mixing structure includes a first mixing chamber, a second mixing chamber, a third mixing chamber and a fourth mixing chamber which are connected in sequence, and an angle is formed between the wall surface of the first mixing chamber and the first pipe body, the inner wall surface of the second mixing chamber is a curved surface, the third mixing chamber is formed with at least two arc-shaped channels, and an angle is formed between the wall surface of the fourth mixing chamber and the second pipe body. The detection device for microorganisms in traditional Chinese medicine according to the embodiment of the present application realizes the detection of microorganisms in traditional Chinese medicine.

Description

Technical Field

[0001] The present application relates to the technical field of microbial detection in traditional Chinese medicine, and in particular to a device and method for detecting microbial detection in traditional Chinese medicine. Background Art

[0002] Traditional Chinese medicine is an important part of the traditional Chinese medicine industry and plays an important role in disease prevention and treatment. The identification of microorganisms in traditional Chinese medicine currently mainly includes microbial culture method and DNA sequencing method. The microbial culture method is time-consuming, has many influencing factors, and requires a high level of technology. The DNA sequencing method requires PCR technology and commercial equipment, which has certain advantages over the culture method, but the cost is high, has great limitations, and relies on large instruments or other necessary laboratory conditions. That is, among the relevant technologies, the convenience of microbial detection in traditional Chinese medicine is not enough. Summary of the invention

[0003] The present application aims to solve at least one of the technical problems existing in the related art. To this end, the present application proposes a Chinese medicine microorganism detection device, which realizes the detection of Chinese medicine microorganisms, and can complete the detection without a complex pumping system, making the detection process more convenient and faster, reducing the skill requirements of the operator, and saving the detection cost.

[0004] The application also proposes a method for detecting microorganisms in traditional Chinese medicine.

[0005] According to the first aspect of the present application, the Chinese medicine microorganism detection device comprises:

[0006] A chip body, wherein the chip body is formed with a liquid inlet, a mixing pipe, a detection chamber and a liquid outlet which are connected in sequence;

[0007] A magnetic component, disposed in the detection chamber;

[0008] A peristaltic pump connected to the liquid outlet;

[0009] Wherein, the mixing pipe includes a first pipe body, a second pipe body and a mixing structure located between the first pipe body and the second pipe body, the mixing structure includes a first mixing chamber, a second mixing chamber, a third mixing chamber and a fourth mixing chamber connected in sequence, the first mixing chamber is connected to the first pipe body, an angle is formed between the wall surface of the first mixing chamber and the first pipe body, the inner wall surface of the second mixing chamber is a curved surface, the third mixing chamber forms at least two arc-shaped channels, the fourth mixing chamber is connected to the second pipe body, an angle is formed between the wall surface of the fourth mixing chamber and the second pipe body.

[0010] According to the Chinese medicine microorganism detection device of the embodiment of the present application, the peristaltic pump can drive the liquid to flow along the liquid inlet and the mixing pipe, and finally flow out from the liquid outlet. When performing Chinese medicine microorganism detection, the immunomagnetic beads, the immunoplatinum particle mixture and the solution containing Chinese medicine microorganisms are transported to the mixing pipe through the liquid inlet, and flow into the detection chamber after mixing in the mixing pipe. Then the peristaltic pump stops working, and the magnetic component is used to apply a magnetic field to the solution in the detection chamber. Then the cleaning agent is sent from the liquid inlet along the mixing pipe into the detection chamber through the peristaltic pump, and then the color developer is sent from the liquid inlet along the mixing pipe into the detection chamber through the peristaltic pump. Then, the image of the detection chamber is obtained and corresponding analysis and processing are performed to obtain the detection result of Chinese medicine microorganisms. The detection of Chinese medicine microorganisms is realized by using microfluidic technology, and the detection can be completed without a complicated pumping system, making the detection process more convenient and faster, reducing the skill requirements of the operator, and saving the detection cost.

[0011] Moreover, when the liquid flows in the mixing pipe, when the liquid flows from the first pipe body to the first mixing chamber, since an angle is formed between the first pipe body and the wall of the first mixing chamber, that is, a corner is provided at the first pipe body and the first mixing chamber, the liquid will be blocked at the corner, so that the liquid will reverse and collide with the subsequent liquid flowing over, generating a small-scale vortex, thereby promoting the mixing of the liquid; when the liquid flows from the first mixing chamber to the second mixing chamber, since the wall of the second mixing chamber is a curved surface, the liquid will generate a secondary flow on the cross section perpendicular to the flow direction, thereby enhancing the chaotic process of the liquid. degree, thus improving the mixing effect of the liquid; when the liquid flows from the second mixing chamber to the third mixing chamber, the liquid will be divided into at least two parts, respectively flowing from different arc channels to the fourth mixing chamber, and the liquids at different arc channels will collide at the fourth mixing chamber, further improving the mixing effect of the liquid; when the liquid flows from the fourth mixing chamber to the second pipe body, due to the angle formed between the wall of the fourth mixing chamber and the second pipe body, that is, the second pipe body and the fourth mixing chamber are provided with a corner, the liquid will be blocked at the corner, thereby generating a small range of eddies to promote the mixing of the liquid. That is, after the liquid passes through the mixing pipe, it can be fully mixed to ensure that subsequent detection can be carried out smoothly.

[0012] According to an embodiment of the present application, a liquid flow direction in the first pipe body and a liquid flow direction in the first mixing chamber are perpendicular to each other.

[0013] According to one embodiment of the present application, at least two of the arc-shaped channels are connected to the inlet of the third mixing chamber.

[0014] According to one embodiment of the present application, at least two of the arc-shaped channels have different widths.

[0015] According to one embodiment of the present application, the cross-sectional areas of the same arc-shaped channel at different positions are different.

[0016] According to one embodiment of the present application, the fourth mixing chamber includes a first mixing section and a second mixing section connected in sequence, the first mixing section connects the second mixing section and the third mixing chamber, and the cross-sectional area of ​​the first mixing section is smaller than the cross-sectional area of ​​the second mixing section.

[0017] According to one embodiment of the present application, the mixing pipeline includes at least one row of the mixing structures, and the mixing structures in the same row are staggered with each other.

[0018] According to one embodiment of the present application, the Chinese medicine microorganism detection device includes an ultrasonic pool and a multi-way valve, the ultrasonic pool is suitable for cleaning Chinese medicinal materials or Chinese herbal medicines and dissolving Chinese medicine preparations, the outlet of the multi-way valve is connected to the liquid inlet, and the multi-way valve is suitable for conveying different solutions to the liquid inlet, wherein one of the inlets of the multi-way valve is connected to the ultrasonic pool.

[0019] According to an embodiment of the present application, the magnetic component includes Permalloy and at least two sector-shaped magnets, and the two sector-shaped magnets are disposed around the Permalloy.

[0020] According to the second aspect of the present application, the method for detecting microorganisms in traditional Chinese medicine comprises:

[0021] Placing Chinese medicinal materials, Chinese medicinal pieces or Chinese medicinal preparations in an ultrasonic pool, adding distilled water to the ultrasonic pool, and controlling the ultrasonic pool to ultrasonically clean the Chinese medicinal materials and Chinese medicinal pieces or dissolve the Chinese medicinal preparations;

[0022] The mixed solution of the immunomagnetic beads and the immunoplatinum particles and the solution in the ultrasonic pool are sucked into the mixing pipe by a peristaltic pump, and the mixed solution and the solution in the ultrasonic pool are mixed and then sent into the detection chamber;

[0023] Sending the cleaning agent into the detection chamber along the mixing pipe through the peristaltic pump;

[0024] Delivering the developer into the detection chamber via the peristaltic pump;

[0025] The image of the detection chamber is acquired and analyzed through a mobile phone app.

[0026] Additional aspects and advantages of the present application will be given in part in the description below, and in part will become apparent from the description below, or will be learned through the practice of the present application. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the related technologies, the drawings required for use in the embodiments or the related technical descriptions are briefly introduced below. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0028] Figure 1 It is a schematic diagram of the structure of the traditional Chinese medicine microorganism detection device provided in the embodiment of the present application;

[0029] Figure 2 It is a schematic diagram of the structure of the chip body of the traditional Chinese medicine microorganism detection device provided in the embodiment of the present application;

[0030] Figure 3 It is a partial structural schematic diagram of the hybrid structure of the traditional Chinese medicine microorganism detection device provided in the embodiment of the present application.

[0031] Reference numerals:

[0032] 1. Chip body; 2. Magnetic component; 3. Peristaltic pump; 4. Ultrasonic pool; 5. Multi-way valve;

[0033] 11. Liquid inlet; 12. Mixing pipe; 13. Detection chamber; 14. Liquid outlet;

[0034] 121. first pipe body; 122. second pipe body; 123. mixing structure;

[0035] 1231, first mixing chamber; 1232, second mixing chamber; 1233, third mixing chamber;

[0036] 1234. A fourth mixing chamber; 1235. An arc-shaped channel. DETAILED DESCRIPTION

[0037] The following is a further detailed description of the implementation of the present application in conjunction with the accompanying drawings and examples. The following examples are used to illustrate the present application but cannot be used to limit the scope of the present application.

[0038] In the description of the embodiments of the present application, it should be noted that the terms "center", "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside" and the like indicate positions or positional relationships based on the positions or positional relationships shown in the accompanying drawings, which are only for the convenience of describing the embodiments of the present application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the embodiments of the present application. In addition, the terms "first", "second", and "third" are used for descriptive purposes only and cannot be understood as indicating or implying relative importance.

[0039] In the description of the embodiments of the present application, it should be noted that, unless otherwise clearly specified and limited, the terms "connected" and "connection" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium. For ordinary technicians in this field, the specific meanings of the above terms in the embodiments of the present application can be understood according to specific circumstances.

[0040] In the embodiments of the present application, unless otherwise clearly specified and limited, a first feature being "above" or "below" a second feature may mean that the first and second features are in direct contact, or the first and second features are in indirect contact through an intermediate medium. Moreover, a first feature being "above", "above" or "above" a second feature may mean that the first feature is directly above or obliquely above the second feature, or simply means that the first feature is higher in level than the second feature. A first feature being "below", "below" or "below" a second feature may mean that the first feature is directly below or obliquely below the second feature, or simply means that the first feature is lower in level than the second feature.

[0041] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the embodiments of the present application. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art may combine and combine the different embodiments or examples described in this specification and the features of the different embodiments or examples, without contradiction.

[0042] Combine the following Figures 1 to 3 The present invention describes the device and method for detecting microorganisms in traditional Chinese medicine.

[0043] According to the embodiment of the first aspect of the present application, Figure 1 , Figure 2 and Figure 3As shown, the Chinese medicine microorganism detection device includes a chip body 1, a magnetic component 2 and a peristaltic pump 3. The chip body 1 is formed with a liquid inlet 11, a mixing pipe 12, a detection chamber 13 and a liquid outlet 14 which are connected in sequence. The magnetic component 2 is arranged at the detection chamber 13, and the peristaltic pump 3 is connected to the liquid outlet 14; wherein the mixing pipe 12 includes a first pipe body 121, a second pipe body 122 and a mixing structure 123 located between the first pipe body 121 and the second pipe body 122, and the mixing structure 123 includes a first mixing chamber 121 connected in sequence, a second mixing chamber 122 and a second mixing chamber 123 connected in sequence, and a first mixing chamber 121 connected in sequence, a second mixing chamber 122 connected in sequence, and a second mixing chamber 123 connected in sequence. 1231, a second mixing chamber 1232, a third mixing chamber 1233 and a fourth mixing chamber 1234, the first mixing chamber 1231 is connected to the first pipe body 121, an angle is formed between the wall surface of the first mixing chamber 1231 and the first pipe body 121, the inner wall surface of the second mixing chamber 1232 is a curved surface, the third mixing chamber 1233 forms at least two arc-shaped channels 1235, the fourth mixing chamber 1234 is connected to the second pipe body 122, an angle is formed between the wall surface of the fourth mixing chamber 1234 and the second pipe body 122.

[0044] According to the Chinese medicine microorganism detection device of the embodiment of the present application, the peristaltic pump 3 can drive the liquid to flow along the liquid inlet 11 and the mixing pipe 12, and finally flow out from the liquid outlet 14. When performing Chinese medicine microorganism detection, the immunomagnetic beads, the immunoplatinum particle mixture and the solution with Chinese medicine microorganisms are transported to the mixing pipe 12 through the liquid inlet 11, and flow into the detection chamber 13 after mixing in the mixing pipe 12. Then the peristaltic pump 3 stops working, and the magnetic component 2 is used to apply a magnetic field to the solution in the detection chamber 13. Then the cleaning agent is sent from the liquid inlet to the detection chamber 13 along the mixing pipe 12 through the peristaltic pump 3, and then the color developer is sent from the liquid inlet to the detection chamber 13 along the mixing pipe 12 through the peristaltic pump 3. Then, the image of the detection chamber 13 is obtained and the corresponding analysis and processing can be performed to obtain the detection result of the Chinese medicine microorganism, thereby realizing the detection of Chinese medicine microorganisms using microfluidic technology, and the detection can be completed without a complex pumping system, making the detection process more convenient and faster, reducing the skill requirements of the operator, and saving the detection cost.

[0045] Moreover, when the liquid flows in the mixing pipe 12, when the liquid flows from the first pipe body 121 into the first mixing chamber 1231, since an angle is formed between the first pipe body 121 and the wall of the first mixing chamber 1231, that is, a corner is provided at the first pipe body 121 and the first mixing chamber 1231, the liquid will be blocked at the corner, so that the liquid will reverse and collide with the subsequent liquid flowing over, generating a small-scale vortex, thereby promoting the mixing of the liquid; when the liquid flows from the first mixing chamber 1231 into the second mixing chamber 1232, since the wall of the second mixing chamber 1232 is a curved surface, the liquid will generate a secondary flow on the cross section perpendicular to the flow direction, thereby enhancing the degree of chaos of the liquid and improving Liquid mixing effect: When the liquid flows from the second mixing chamber 1232 to the third mixing chamber 1233, the liquid will be divided into at least two parts, and flow from different arc channels 1235 to the fourth mixing chamber 1234 respectively. The liquids at different arc channels 1235 will collide at the fourth mixing chamber 1234, further improving the liquid mixing effect; when the liquid flows from the fourth mixing chamber 1234 to the second pipe body 122, due to the angle formed between the wall of the fourth mixing chamber 1234 and the second pipe body 122, that is, the second pipe body 122 and the fourth mixing chamber 1234 are provided with a corner, the liquid will be blocked at the corner, and then a small range of eddies will be generated to promote the mixing of the liquid. That is, after the liquid passes through the mixing pipe 12, it can be fully mixed to ensure that the subsequent detection can be carried out smoothly.

[0046] In one embodiment of the present application, the liquid flow direction in the first pipe body 121 and the liquid flow direction in the first mixing chamber 1231 are perpendicular to each other.

[0047] It can be understood that the angle between the wall of the first pipe body 121 and the first mixing chamber 1231 is set to 90 degrees, so that a right-angle corner channel is formed at the connection between the first pipe body 121 and the first mixing chamber 1231, which can block the liquid flowing through and generate a small-scale vortex.

[0048] In one embodiment of the present application, at least two arc-shaped channels 1235 are both connected to the inlet of the third mixing chamber 1233 .

[0049] It can be understood that the two arc channels 1235 are connected to the entrance of the third mixing chamber 1233 at the same time, that is, the liquids at the two arc channels 1235 will flow to the entrance of the third mixing chamber 1233, so that the liquids at the two arc channels 1235 will collide when flowing into the third mixing chamber 1233, thereby improving the degree of mixing of the liquids.

[0050] It is understandable that at least two arc channels 1235 may share one outlet, that is, the liquid in the two arc channels 1235 will flow from the same outlet to the third mixing chamber 1233, thereby ensuring that the liquid in the two arc channels 1235 will eventually collide with each other, ensuring the liquid mixing effect.

[0051] In one embodiment of the present application, at least two arc-shaped channels 1235 have different widths.

[0052] It is understandable that the cross-sectional areas of at least two arc-shaped channels 1235 are set to be different, that is, the cross-sectional areas of the two arc-shaped channels 1235 are of different sizes, so that the liquids in the two arc-shaped channels 1235 will collide strongly when flowing into the third mixing chamber 1233, thereby improving the mixing degree of the liquids.

[0053] In one embodiment of the present application, the cross-sectional areas of the same arc-shaped channel 1235 at different positions are different.

[0054] It can be understood that when the liquid flows along the arc channel 1235, due to the different cross-sectional areas at different positions of the arc channel 1235, the liquid flowing along the arc channel 1235 will experience flowing from a position with a larger cross-sectional area to a position with a smaller cross-sectional area, and / or, from a position with a smaller cross-sectional area to a position with a larger cross-sectional area, both of which can promote the mixing of the liquid and improve the degree of mixing of the liquid.

[0055] In one embodiment of the present application, the fourth mixing chamber 1234 includes a first mixing section and a second mixing section connected in sequence, the first mixing section connects the second mixing section and the third mixing chamber 1233, and the cross-sectional area of ​​the first mixing section is smaller than the cross-sectional area of ​​the second mixing section.

[0056] It can be understood that when the liquid flows along the fourth mixing chamber 1234, the liquid will flow from the first mixing section to the second mixing section. Since the cross-sectional area of ​​the first mixing section is smaller than the cross-sectional area of ​​the second mixing section, the flow path of the liquid will become larger when the liquid flows from the first mixing section to the second mixing section. The sudden enlargement of the flow path will cause the liquid to generate vortexes, thereby effectively improving the mixing degree of the liquid.

[0057] In one embodiment of the present application, Figure 2 As shown, the mixing pipe 12 includes at least one row of mixing structures 123, and the mixing structures 123 in the same row are staggered with each other.

[0058] It is understandable that by providing a plurality of mixing structures 123 and connecting the plurality of mixing structures 123 in a row, the mixing pipe 12 can mix the liquid multiple times, thereby ensuring the mixing degree of the liquid. Furthermore, the mixing structures 123 are arranged staggered with each other, and when the liquid flows from the previous mixing structure 123 to the next mixing structure 123, the flow direction of the liquid changes, further improving the mixing degree of the liquid.

[0059] Exemplarily, when liquid flows from a previous mixing structure 123 to a next mixing structure 123 , the liquid flow direction at the first mixing chamber 1231 of the previous mixing structure 123 is opposite to the liquid flow direction at the first mixing chamber 1231 of the next mixing structure 123 .

[0060] In one embodiment of the present application, Figure 1 As shown, the Chinese medicine microorganism detection device includes an ultrasonic pool 4 and a multi-way valve 5. The ultrasonic pool 4 is suitable for cleaning Chinese medicinal materials or Chinese medicinal pieces and dissolving Chinese medicine preparations. The outlet of the multi-way valve 5 is connected to the liquid inlet 11. The multi-way valve 5 is suitable for conveying different solutions to the liquid inlet 11, wherein one of the inlets of the multi-way valve 5 is connected to the ultrasonic pool 4.

[0061] It can be understood that the Chinese medicinal materials, Chinese medicinal pieces or Chinese medicinal preparations are placed in the ultrasonic pool 4, and the ultrasonic pool 4 is used to clean the Chinese medicinal materials or Chinese medicinal pieces and dissolve the Chinese medicinal preparations, so that the liquid in the ultrasonic pool 4 contains Chinese medicinal microorganisms for subsequent detection.

[0062] It is understandable that different liquids can flow from different inlets of the multi-way valve 5 to the liquid inlet 11 under the action of the peristaltic pump 3, so as to complete the entire Chinese medicine microbial detection process. Through the cooperation of the peristaltic pump 3 and the multi-way valve 5, the type and flow rate of the solution flowing to the liquid inlet 11 can be controlled.

[0063] In one embodiment of the present application, the magnetic component 2 includes Permalloy and at least two sector-shaped magnets, and the two sector-shaped magnets are surrounded by the Permalloy.

[0064] It can be understood that the combination of the fan-shaped magnet and Permalloy can generate a magnetic field, so that the detection chamber 13 is within the magnetic field range. During the detection process, substances such as the double antibody sandwich will be produced in the detection chamber 13, and the magnetic component 2 can adsorb the magnetic beads and fix the double antibody sandwich.

[0065] It can be understood that in order to improve the enrichment rate of the double antibody sandwich complex formed in the detection chamber 13, the present application: uses two radially magnetized, mutually repelling sector magnets to surround the Permalloy, and the immunomagnetic beads in the solution are subjected to the magnetic force in the magnetic field and tend to move along the magnetic flux lines in the direction of increasing magnetic field gradient, and the magnetic force received by a single immunomagnetic bead in the magnetic field can be expressed as Where x is the difference in volume magnetic susceptibility between the immunomagnetic beads and the fluid; μ0 is the vacuum magnetic permeability, which is a constant 4π×10 -7 N / A 2 ; V represents the volume of immunomagnetic beads; H and Represent the strength and gradient of the magnetic field respectively. The diameter of the magnetic beads used in the study is 180nm, and the volume magnetic susceptibility is about 350emu / cm 3 Since the volume magnetic susceptibility of the fluid is much smaller than that of the magnetic beads, the volume magnetic susceptibility of the magnetic beads can be approximated as the difference between the volume magnetic susceptibilities of the immunomagnetic beads and the fluid.

[0066] Under this condition, the magnetic force on the magnetic beads can be enhanced by increasing the magnetic field strength and gradient. The N52 neodymium iron boron magnet with the strongest magnetic properties so far was selected as a component of the high gradient magnetic field. In terms of magnetic field gradient, the magnetic field simulation experiment of the high gradient magnetic field was carried out using COMSOL Multiphysics multi-physics field simulation software. It can be seen that the magnetic flux density increases significantly from the outer edge of the magnet to the inside, indicating that the magnetic field gradient in this area is large. High magnetic field strength and gradient will effectively enhance the magnetic force on the magnetic beads in the solution, thereby achieving a high enrichment rate of magnetic beads. Using FEMM4.2 to simulate the magnetic field strength, it can be found that the high-intensity position of the magnetic field is distributed in a point-like manner and is located inside the reaction pool. The magnetic field can be used to gather the magnetic beads in a point-like manner. The magnetic field simulation experiment was carried out using COMSOL Multiphysics multi-physics field simulation software to obtain the magnetic field strength at 1mm. Comparing this data with the magnetic field strength at the same position of a cylindrical magnet of the same specification, the magnetic field strength is significantly higher than that of a cylindrical magnet. Therefore, this magnetic field has a higher enrichment ability for magnetic beads and can concentrate the magnetic beads.

[0067] According to an embodiment of the second aspect of the present application, a method for detecting microorganisms in traditional Chinese medicine comprises:

[0068] S100, placing Chinese medicinal materials, Chinese medicinal pieces or Chinese medicinal preparations in an ultrasonic pool 4, adding distilled water to the ultrasonic pool 4, and controlling the ultrasonic pool 4 to perform ultrasonic cleaning on the Chinese medicinal materials, Chinese medicinal pieces or Chinese medicinal preparations;

[0069] S200, the mixed solution of the immunomagnetic beads and the immunoplatinum particles and the solution in the ultrasonic pool 4 are sucked into the mixing pipe 12 through the peristaltic pump 3, and the mixed solution and the solution in the ultrasonic pool 4 are mixed and then sent into the detection chamber 13;

[0070] S300, sending the cleaning agent into the detection chamber 13 along the mixing pipe 12 through the peristaltic pump 3;

[0071] S400, delivering the color developing agent into the detection chamber 13 through the peristaltic pump 3;

[0072] S500 , acquiring an image of the detection chamber 13 , and analyzing it through a mobile phone app.

[0073] It can be understood that an appropriate amount of Chinese medicinal materials, Chinese medicinal pieces or Chinese medicinal preparations are placed in the ultrasonic pool 4, distilled water is added to the ultrasonic pool 4 so that the distilled water submerges the Chinese medicinal materials, Chinese medicinal pieces or Chinese medicinal preparations, and then the ultrasonic pool 4 is controlled to work at 40KHz ultrasound for three minutes to achieve the cleaning of the Chinese medicinal materials, Chinese medicinal pieces or Chinese medicinal preparations, so that the liquid in the ultrasonic pool 4 carries Chinese medicinal microorganisms.

[0074] Then, 50 microliters of ultrasonic liquid, 20 microliters of immunomagnetic beads, and 30 microliters of a mixed solution of immunoplatinum particles are extracted by the peristaltic pump 3, and the solution is mixed in the mixing pipe 12 and transported to the detection chamber 13, and then the peristaltic pump 3 stops working for a certain period of time.

[0075] Then, the cleaning agent is delivered into the detection chamber 13 along the mixing pipe 12 by the peristaltic pump 3 , so as to clean the mixing pipe 12 and the detection chamber 13 .

[0076] The developer is then transported to the detection chamber 13 by the peristaltic pump 3, and finally an image of the detection chamber 13 is acquired by taking a photo with a mobile phone, and the image is uploaded to the mobile phone app for analysis to obtain the final detection result.

[0077] Finally, it should be noted that the above implementation modes are only used to illustrate the present application, rather than to limit the present application. Although the present application is described in detail with reference to the embodiments, a person skilled in the art should understand that various combinations, modifications or equivalent substitutions of the technical solutions of the present application do not depart from the spirit and scope of the technical solutions of the present application, and should be included in the scope of the claims of the present application.

Claims

1. A Chinese medicine microorganism detection device, characterized in that: include: A chip body, wherein the chip body is formed with a liquid inlet, a mixing pipe, a detection chamber and a liquid outlet which are connected in sequence; A magnetic component, disposed in the detection chamber; A peristaltic pump connected to the liquid outlet; Wherein, the mixing pipeline comprises a first pipeline body, a second pipeline body and a mixing structure located between the first pipeline body and the second pipeline body, the mixing structure comprises a first mixing chamber, a second mixing chamber, a third mixing chamber and a fourth mixing chamber connected in sequence, the first mixing chamber is communicated with the first pipeline body, an angle is formed between the wall surface of the first mixing chamber and the first pipeline body, the inner wall surface of the second mixing chamber is a curved surface, the third mixing chamber forms at least two arc-shaped channels, the fourth mixing chamber is communicated with the second pipeline body, an angle is formed between the wall surface of the fourth mixing chamber and the second pipeline body; At least two of the arc-shaped channels share one outlet, which ensures that the liquids in the two arc-shaped channels will eventually collide with each other, thereby ensuring a liquid mixing effect.

2. The Chinese medicine microorganism detection device according to claim 1, characterized in that: A liquid flow direction in the first pipe body and a liquid flow direction in the first mixing chamber are perpendicular to each other.

3. The Chinese medicine microorganism detection device according to claim 1, characterized in that: At least two of the arc-shaped channels are communicated with the inlet of the third mixing chamber.

4. The Chinese medicine microorganism detection device according to claim 1, characterized in that: At least two of the arc-shaped channels have different widths.

5. The Chinese medicine microorganism detection device according to claim 1, characterized in that: The cross-sectional areas of the same arc-shaped channel at different positions are different.

6. The Chinese medicine microorganism detection device according to any one of claims 1 to 5, characterized in that: The fourth mixing chamber includes a first mixing section and a second mixing section which are connected in sequence, the first mixing section is connected to the second mixing section and the third mixing chamber, and the cross-sectional area of ​​the first mixing section is smaller than the cross-sectional area of ​​the second mixing section.

7. The Chinese medicine microorganism detection device according to any one of claims 1 to 5, characterized in that: The mixing pipeline includes at least one row of the mixing structures, and the mixing structures in the same row are staggered with each other.

8. The Chinese medicine microorganism detection device according to any one of claims 1 to 5, characterized in that: The Chinese medicine microorganism detection device includes an ultrasonic pool and a multi-way valve. The ultrasonic pool is suitable for cleaning Chinese medicinal materials or Chinese medicinal pieces and dissolving Chinese medicine preparations. The outlet of the multi-way valve is connected to the liquid inlet. The multi-way valve is suitable for conveying different solutions to the liquid inlet, wherein one of the inlets of the multi-way valve is connected to the ultrasonic pool.

9. The Chinese medicine microorganism detection device according to any one of claims 1 to 5, characterized in that: The magnetic component includes permalloy and at least two sector-shaped magnets, and the two sector-shaped magnets are arranged around the permalloy.

10. A method for detecting microorganisms in traditional Chinese medicine based on the device for detecting microorganisms in traditional Chinese medicine according to any one of claims 1 to 9, characterized in that: include: Placing Chinese medicinal materials, Chinese medicinal pieces or Chinese medicinal preparations in an ultrasonic pool, adding distilled water to the ultrasonic pool, and controlling the ultrasonic pool to ultrasonically clean the Chinese medicinal materials and Chinese medicinal pieces or dissolve the Chinese medicinal preparations; The mixed solution of the immunomagnetic beads and the immunoplatinum particles and the solution in the ultrasonic pool are sucked into the mixing pipe by a peristaltic pump, and the mixed solution and the solution in the ultrasonic pool are mixed and then sent into the detection chamber; Sending the cleaning agent into the detection chamber along the mixing pipe through the peristaltic pump; Delivering the developer into the detection chamber via the peristaltic pump; The image of the detection chamber is acquired and analyzed through a mobile phone app.

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