A shampoo capable of promoting hair growth and preventing hair loss and a preparation method thereof

Abstract

The invention discloses a shampoo capable of promoting hair growth and preventing hair loss and a preparation method thereof. The shampoo is composed of the following raw materials in mass percentage: 3-6% of apigenin composite nanoparticles, 2-4% of proanthocyanidin B2 liposomes, 10-15% of surfactants, 2-8% of moisturizers, 3-5% of thickeners, 0.05-0.1% of chelating agents, 0.5-1% of preservatives, 0.1-0.5% of essences, and the balance is deionized water. The invention uses Brazilian nut protein hydrolyzate nanoparticles with high apigenin loading and proanthocyanidin B2 nanoliposomes as effective ingredients, and the prepared shampoo not only has excellent effects of promoting hair growth and preventing hair loss, but also has good compatibility stability with conventional bases of shampoo formulas, and has broad application prospects.

Description

Technical Field

[0001] The invention belongs to the technical field of daily washing and care products, and particularly relates to a shampoo capable of promoting hair growth and preventing hair loss and a preparation method thereof. Background Art

[0002] As people's living standards improve, their requirements for hair care products are getting higher and higher. However, with the accelerated pace of life, increased work pressure, and deteriorating living environment, more and more people are experiencing hair breakage, hair loss, and hair loss, and more and more people are experiencing thin and soft hair. Existing anti-hair loss products mainly add chemical drugs that have the effect of removing oil and stimulating hair follicles. They can clean the scalp and stimulate hair follicles in the short term, but long-term use will have a counterproductive effect. Some shampoos made from pure Chinese herbal plant preparations do not have a significant effect on stopping hair loss and promoting hair growth.

[0003] Apigenin is a poorly soluble flavonoid substance and is a known main ingredient for alleviating hair loss. It can stimulate hair growth through various biological pathways, thereby increasing hair thickness, reducing the appearance of gray hair, and providing a fuller hair appearance. However, when apigenin is applied to existing cosmetic compositions, it is difficult to prepare a composition in which an effective amount of apigenin is dissolved due to its insolubility and precipitation problems during long-term storage. On the other hand, although it is known that there is a technology for dissolving apigenin by using a large amount of ethanol, surfactants and polyols, when such a composition is used, the problem is that it is not only difficult to provide a refreshing feeling of use, but also causes excessive scalp irritation due to the high content of ethanol.

[0004] Proanthocyanidin B2 is a natural flavonoid, a natural polyphenolic compound composed of two epicatechins. Studies have shown that proanthocyanidin B2 can mediate a variety of biochemical and pharmacological effects, including antioxidant, lipid-lowering, anti-inflammatory and anti-apoptotic activities. However, due to the water solubility of proanthocyanidin B2, its ability to penetrate into the hair follicles is limited. In addition, it is very unstable and sensitive to light. It is easily decomposed under alkaline and high temperature (>40°C) conditions. Metal ions (Cu 2+ , Ca 2+ 、Zn 2+ ) and preservatives (potassium sorbate, sodium sulfite) will affect its stability, resulting in low compatibility in the formula and weakened effect.

[0005] At present, there is no report on the combined use of apigenin and proanthocyanidin B2 in the preparation of hair care products for promoting hair growth and preventing hair loss. Summary of the invention

[0006] In view of the deficiencies of the above prior art, the present invention provides a shampoo capable of promoting hair growth and preventing hair loss and a preparation method thereof. The shampoo uses apigenin composite nanoparticles and proanthocyanidin B2 liposomes as active ingredients, which not only has a significant effect of promoting hair growth and preventing hair loss, but also effectively solves the problems of poor stability and poor compatibility of apigenin and proanthocyanidin B2 in the preparation of toiletries.

[0007] The technical solution of the present invention is:

[0008] A shampoo capable of promoting hair growth and preventing hair loss is composed of the following raw materials in percentage by mass: 3-6% of apigenin composite nanoparticles, 2-4% of proanthocyanidin B2 liposomes, 10-15% of surfactants, 2-8% of moisturizers, 3-5% of thickeners, 0.05-0.1% of chelating agents, 0.5-1% of preservatives, 0.1-0.5% of flavors, and the balance being deionized water.

[0009] Furthermore, the apigenin composite nanoparticles are Brazilian nut protein hydrolyzate nanoparticles loaded with apigenin, and the apigenin loading amount is 10-100 mg / g.

[0010] Furthermore, the apigenin composite nanoparticles are prepared by the following method: dissolving Brazilian nut protein in water to prepare a protein solution with a mass fraction of 7-8%, then adding papain with a mass fraction of 1-2.5% of Brazilian nut protein, hydrolyzing for 2-3 hours at a pH of 6-7 and a temperature of 55-65°C, inactivating the enzyme, and centrifuging to obtain a supernatant to obtain a Brazilian nut protein hydrolyzate; adjusting the pH of the Brazilian nut protein hydrolyzate to 10-12, adding apigenin under stirring conditions, continuing to stir for 1-2 hours, adjusting the pH value of the mixed solution to 6-7, centrifuging to obtain a supernatant, and drying the supernatant to obtain Brazilian nut protein hydrolyzate nanoparticles loaded with apigenin.

[0011] Furthermore, the proanthocyanidin B2 liposome is prepared by the following method:

[0012] S1. Mix hydrogenated lecithin, cholesterol and Tween 80 in a mass ratio of (8-9):(1-2):(1-2), add anhydrous ethanol to prepare a solution A with a concentration of 3-4 mg / mL;

[0013] S2, dissolving proanthocyanidin B2 in a phosphate buffer solution with a pH of 5.0 to prepare a solution B with a concentration of 0.1 to 0.2 mg / mL;

[0014] S3. Place solution A in a rotary evaporator to evaporate under reduced pressure to remove ethanol, then let it stand for 5 to 10 minutes, add solution B, and oscillate the hydration reaction in a 35 to 40°C water bath for 1 to 2 hours to obtain a suspension, and ultrasonically oscillate the suspension in an ice bath for 10 to 30 minutes to obtain proanthocyanidin B2 liposomes.

[0015] Furthermore, in S3, the ultrasonic conditions are: working for 2-4s, resting for 3-5s, and power of 200-220W.

[0016] Furthermore, the surfactant includes one or more of sodium laureth sulfate, cocamidopropyl hydroxysulfobetaine, sodium methyl cocoyl taurate, cocamidopropyl betaine, and disodium cocamide glutamate.

[0017] Furthermore, the moisturizing agent includes one or more of glycerin, butylene glycol, squalane, sodium hyaluronate, fructan, and trehalose.

[0018] Furthermore, the thickener includes one or more of hydroxyethyl cellulose, carbomer, acrylates / C10-30 alkyl acrylate cross-linked polymer, xanthan gum, ceteareth-60 myristyl glycol, and sodium acrylate / sodium acryloyldimethyl taurate copolymer.

[0019] Furthermore, the chelating agent includes one or more of EDTA-2Na, tetrasodium glutamate diacetate, trisodium ethylenediamine disuccinate, and tetrasodium methylglycine diacetate.

[0020] Furthermore, the preservative is a mixture of phenoxyethanol and ethylhexylglycerin, methylparaben or ethylparaben.

[0021] The present invention also provides a method for preparing the above-mentioned shampoo, comprising the following steps: dissolving a surfactant, a moisturizing agent, a thickener and a chelating agent in deionized water, heating to 80-85° C. in a vacuum batching pot, keeping warm and stirring for 20-40 minutes, cooling to 40-45° C., adding apigenin composite nanoparticles, proanthocyanidin B2 liposomes, a preservative and essence, stirring evenly, and adjusting the pH to 6-7, and discharging the material to obtain the shampoo.

[0022] The present invention has the following beneficial effects:

[0023] Brazilian nut protein hydrolysate is an enzymatic hydrolysate of Brazilian nut fruit protein. The amino acids containing sulfur bonds and disulfide bonds in the protein are superior to other plant proteins, and the protein is rich in selenium, which is beneficial to healthy hair growth and reduces the occurrence of hair loss. The present invention utilizes the high reactivity of the hydrophobic groups in the Brazilian nut protein hydrolysate, which is easy to interact with the hydrophobic active factors. It is used as an embedding carrier under specific pH conditions to embed the hydrophobic apigenin therein through molecular self-assembly technology to obtain Brazilian nut protein hydrolysate nanoparticles with high apigenin loading, and the apigenin loading is 10-100 mg / g, which not only effectively solves the problem of apigenin being difficult to dissolve and easy to precipitate, but also utilizes the characteristics of Brazilian nut protein hydrolysate being easily absorbed by skin and hair, and rapidly improves the hair follicle permeability of apigenin. Experiments have shown that the effects of Brazilian nut protein hydrolysate nanoparticles with high apigenin loading on promoting hair growth and reducing hair loss are better than the single use effects of Brazilian nut protein hydrolysate and apigenin. Compared with the direct application of apigenin and Brazilian nut protein hydrolysate, the Brazilian nut protein hydrolysate nanoparticles with high apigenin loading have better effects. It is speculated that this is because encapsulating apigenin in Brazilian nut protein hydrolysate can effectively improve the solubility and permeability of apigenin.

[0024] The present invention prepares proanthocyanidin B2 into nanoliposomes, which not only effectively solves the problem of poor stability of proanthocyanidin B2, but also the lecithin and cholesterol components in the liposomes can effectively promote the hair follicle absorption of proanthocyanidin B2, solving the problem of poor hair follicle absorption of proanthocyanidin B2.

[0025] The present invention uses Brazilian nut protein hydrolyzate nanoparticles with high apigenin loading and proanthocyanidin B2 nanoliposomes as effective ingredients. The prepared shampoo not only has excellent effects of promoting hair growth and preventing hair loss, but also has good compatibility stability with conventional bases of shampoo formulas, and has broad application prospects. DETAILED DESCRIPTION

[0026] The following examples are provided to further illustrate the present invention, but are not intended to limit the present invention. The components of the formulations in the following examples are all conventional commercially available products unless otherwise specified.

[0027] The shampoos in the following examples are prepared using the apigenin composite nanoparticles prepared in Example 1 and the proanthocyanidin B2 liposomes prepared in Example 2 as raw materials.

[0028] Example 1

[0029] Preparation of apigenin composite nanoparticles: Brazilian nut protein was dissolved in water to prepare a protein solution with a mass fraction of 8%, and then papain was added to hydrolyze at pH 7 and temperature 60°C for 2.5 hours, and the amount of papain used was 2% of the mass of Brazilian nut protein. After the enzymatic hydrolysis was completed, the temperature was raised to 100°C to inactivate the enzyme for 10 minutes, and the material obtained after enzymatic hydrolysis was centrifuged at 6000rpm and 4°C for 10 minutes to obtain the supernatant to obtain Brazilian nut protein hydrolysate; the pH of the Brazilian nut protein hydrolysate was adjusted to about 12, and apigenin was added under stirring conditions, and the mass ratio of apigenin to Brazilian nut protein was 1:5. After continuing to stir for 2 hours, the pH value of the mixed solution was adjusted to 7, and the supernatant was obtained by centrifugation at 15000rpm and 4°C for 10 minutes, and the supernatant was freeze-dried to obtain Brazilian nut protein hydrolysate nanoparticles loaded with apigenin.

[0030] After testing, the loading amount of apigenin in the apigenin composite nanoparticles was 88.72 mg / g.

[0031] According to the above method, the Brazilian nut protein hydrolyzate is freeze-dried to obtain the Brazilian nut protein hydrolyzate.

[0032] Observation on the solubility of apigenin composite nanoparticles: 20 mg of freeze-dried apigenin-loaded Brazilian nut protein hydrolysate nanoparticles were dissolved in 10 mL of deionized water and stirred to disperse them evenly. After standing for 1 day, the dispersion of the nanoparticles was observed. The results showed that the apigenin-loaded Brazilian nut protein hydrolysate nanoparticles had good solubility and could be dispersed in water to form a uniform dispersion.

[0033] Observation of the stability of apigenin composite nanoparticles: 20 mg of freeze-dried apigenin-loaded Brazilian walnut protein hydrolyzate nanoparticles were dissolved in 10 mL of deionized water and stirred to disperse evenly to prepare a dispersion with a concentration of 2 mg / mL. The storage stability of the apigenin composite nanoparticle dispersion was studied by observing the changes in the particle size and PDI value of the dispersion after fresh re-dissolution and standing for 15 days. The results showed that after standing for 15 days, the particle size of the apigenin composite nanoparticles had almost no change. After standing for 15 days, the PDI value of the nanoparticles decreased, indicating that during the standing process, the distribution uniformity of the nanoparticles in the dispersion became better. The results show that the storage stability of the apigenin composite nanoparticles is good, the particle size has almost no change during the placement process, and the stability and dispersion of the dispersion tend to be better.

[0034] Example 2

[0035] Proanthocyanidin B2 nanoliposomes are prepared by the following method:

[0036] (1) hydrogenated lecithin, cholesterol and Tween 80 were mixed in a mass ratio of 8:2:1, and anhydrous ethanol was added to prepare a solution A with a concentration of 4 mg / mL;

[0037] (2) dissolving proanthocyanidin B2 in a phosphate buffer solution with a pH of 5.0 to prepare a solution B with a concentration of 0.2 mg / mL;

[0038] (3) Solution A was placed in a rotary evaporator to evaporate the ethanol under reduced pressure, and then allowed to stand for 10 min. Solution B was added and oscillated in a 35°C water bath for 2 h to obtain a suspension. The suspension was ultrasonically shaken in an ice bath for 30 min. The ultrasonic conditions were 4 s on, 5 s off, and a power of 200 W to obtain proanthocyanidin B2 liposomes.

[0039] The encapsulation efficiency of proanthocyanidin B2 nanoliposomes was found to be 80.6%.

[0040] Example 3

[0041] A shampoo capable of promoting hair growth and preventing hair loss is composed of the following raw materials in percentage by mass: 5% of apigenin composite nanoparticles, 3% of proanthocyanidin B2 liposomes, 8% of sodium laureth sulfate, 2% of cocamidopropyl hydroxysulfobetaine, 2% of disodium cocamide glutamate, 4% of glycerol, 2% of sodium hyaluronate, 2% of butylene glycol, 2% of xanthan gum, 2% of ceteareth-60 myristyl glycol, 0.05% of EDTA-2Na, 0.9% of phenoxyethanol, 0.1% of ethylhexylglycerin, 0.4% of essence, and the balance is deionized water.

[0042] Preparation steps: dissolve sodium laureth sulfate, cocamidopropyl hydroxysulfobetaine, disodium cocamide glutamate, glycerin, sodium hyaluronate, butylene glycol, xanthan gum, ceteareth-60 myristyl glycol, and EDTA-2Na in deionized water, heat to 80°C in a vacuum batching pot, keep stirring for 30 minutes, cool to 40°C, add apigenin composite nanoparticles, proanthocyanidin B2 liposomes, phenoxyethanol, ethylhexylglycerin and essence, stir evenly, adjust the pH to about 7, and obtain the product.

[0043] Example 4

[0044] A shampoo capable of promoting hair growth and preventing hair loss is composed of the following raw materials in percentage by mass: 6% of apigenin composite nanoparticles, 2% of proanthocyanidin B2 liposomes, 10% of sodium laureth sulfate, 3% of cocamidopropyl hydroxysulfobetaine, 2% of disodium cocamide glutamate, 2% of glycerol, 2% of fructan, 2% of xanthan gum, 1% of ceteareth-60 myristyl glycol, 0.1% of tetrasodium glutamate diacetate, 0.9% of phenoxyethanol, 0.1% of ethylhexylglycerin, 0.5% of essence, and the balance is deionized water.

[0045] Preparation steps: dissolve sodium laureth sulfate, cocamidopropyl hydroxysulfobetaine, disodium cocamide glutamate, glycerin, fructan, xanthan gum, ceteareth-60 myristyl glycol, and tetrasodium glutamate diacetate in deionized water, heat to 85°C in a vacuum batching pot, keep stirring for 30 minutes, cool to 40-45°C, add apigenin composite nanoparticles, proanthocyanidin B2 liposomes, phenoxyethanol, ethylhexylglycerin and essence, stir evenly, adjust the pH to about 7, and obtain the product.

[0046] Example 5

[0047] A shampoo capable of promoting hair growth and preventing hair loss is composed of the following raw materials in percentage by mass: 3% of apigenin composite nanoparticles, 4% of proanthocyanidin B2 liposomes, 6% of sodium laureth sulfate, 2% of cocamidopropyl hydroxysulfobetaine, 2% of disodium cocamide glutamate, 1% of glycerol, 1% of trehalose, 3% of xanthan gum, 2% of ceteareth-60 myristyl glycol, 0.05% of EDTA-2Na, 0.3% of phenoxyethanol, 0.2% of methylparaben, 0.1% of essence, and the balance is deionized water.

[0048] Preparation steps: dissolve sodium laureth sulfate, cocamidopropyl hydroxysulfobetaine, disodium cocamide glutamate, glycerin, trehalose, xanthan gum, ceteareth-60 myristyl glycol, and EDTA-2Na in deionized water, heat to 80°C in a vacuum batching pot, keep stirring for 30 minutes, cool to 40°C, add apigenin composite nanoparticles, proanthocyanidin B2 liposomes, phenoxyethanol, methylparaben and essence, stir evenly, adjust the pH to about 7, and obtain the product.

[0049] Example 6

[0050] A shampoo capable of promoting hair growth and preventing hair loss is composed of the following raw materials in percentage by mass: 4% of apigenin composite nanoparticles, 3% of proanthocyanidin B2 liposomes, 8% of sodium laureth sulfate, 2% of cocamidopropyl hydroxysulfobetaine, 2% of disodium cocamide glutamate, 4% of glycerol, 2% of squalane, 2% of xanthan gum, 2% of ceteareth-60 myristyl glycol, 0.05% of tetrasodium glutamate diacetate, 0.3% of phenoxyethanol, 0.2% of ethylparaben, 0.2% of essence, and the balance is deionized water.

[0051] Preparation steps: dissolve sodium laureth sulfate, cocamidopropyl hydroxysulfobetaine, disodium cocamide glutamate, glycerin, squalane, xanthan gum, ceteareth-60 myristyl glycol, and tetrasodium glutamate diacetate in deionized water, heat to 85°C in a vacuum batching pot, keep stirring for 30 minutes, cool to 40°C, add apigenin composite nanoparticles, proanthocyanidin B2 liposomes, phenoxyethanol, ethylparaben and essence, stir evenly, adjust the pH to about 7, and obtain the product.

[0052] Comparative Example 1

[0053] The difference between Comparative Example 1 and Example 1 is that apigenin is used to replace the apigenin composite nanoparticles, the amount of apigenin used is consistent with the content of apigenin in the apigenin composite nanoparticles, and the other ingredients remain unchanged.

[0054] Comparative Example 2

[0055] The difference between Comparative Example 2 and Example 1 is that the apigenin composite nanoparticles are replaced by Brazilian nut protein hydrolysate, the amount of Brazilian nut protein hydrolysate used is consistent with the content of Brazilian nut protein hydrolysate in the apigenin composite nanoparticles, and the other ingredients remain unchanged.

[0056] Comparative Example 3

[0057] The difference between Comparative Example 3 and Example 1 is that Brazilian nut protein hydrolysate and apigenin (directly added) are used to replace the apigenin composite nanoparticles, and the amounts of the two are consistent with the contents of Brazilian nut protein hydrolysate and apigenin in the apigenin composite nanoparticles, and the other ingredients remain unchanged.

[0058] Comparative Example 4

[0059] The difference between Comparative Example 4 and Example 1 is that no proanthocyanidin B2 liposome is added, and the other ingredients remain unchanged.

[0060] Comparative Example 5

[0061] The difference between Comparative Example 5 and Example 1 is that no apigenin composite nanoparticles are added, and the other ingredients remain unchanged.

[0062] Example 7

[0063] Allergy test (human skin patch test): The shampoos prepared in Examples 3-6 and Comparative Examples 1-5 of the present invention were tested with reference to the relevant requirements and determination methods of the "Human Skin Patch Test" in the 2015 edition of the "Cosmetic Hygiene Standards" issued by the Ministry of Health, and the skin reactions were observed at 0h, 6h, 12h, 24h, and 48h, respectively. The experimental results of all volunteer subjects were negative, and no adverse skin reactions occurred. This shows that the shampoo provided by the present invention does not cause allergic reactions, is non-irritating, and has high safety.

[0064] Example 8

[0065] Female volunteers with symptoms of hair loss were selected, aged 30 to 45 years old. The subjects were randomly divided into 9 groups, 8 cases in each group. An area of ​​1.5 cm × 1.5 cm was selected at the back of the subject's head as an observation point, and the hair in the area was removed cleanly with a shaver, while ensuring that the length of the hair near the observation point can cover the shaved area without affecting the appearance. Then, the shampoo prepared in Examples 3-6 and Comparative Examples 1-5 was used to wash the hair. After the hair was wet, an appropriate amount of shampoo was rubbed to make foam, and evenly applied to the hair and scalp, and then massaged in circles with the fingertips, gently rubbing from the roots to the ends of the hair for 3-5 minutes, and then rinsed with clean water. Use once every other day for 2 consecutive months. During the test, no other cosmetics, drugs or health products that may affect the results were used. After 2 months of use, the new hair in the observation point area was cut and weighed, and 10 hairs were randomly selected for length measurement, and the average value was taken. The results are shown in Table 1. The changes in the number of hair loss before and after use of the subjects were counted, and the average value was taken. The results are shown in Table 2.

[0066] Table 1 Average hair length and hair weight

[0067] Group Average hair length (cm) Average hair weight (mg) Example 3 4.6 72.6 Example 4 4.4 69.8 Example 5 3.9 63.0 Example 6 4.0 65.2 Comparative Example 1 3.1 48.4 Comparative Example 2 2.3 37.7 Comparative Example 3 3.4 54.2 Comparative Example 4 2.7 44.6 Comparative Example 5 2.0 32.7

[0068] It can be seen from Table 1 above that the shampoos prepared in Examples 3-6 of the present invention can effectively promote hair growth and make hair thicker and longer, and the effects are better than those of the shampoos prepared in Comparative Examples 1-5.

[0069] Table 2 Changes in the number of hair loss (roots)

[0070] Group Before use After use Improvement rate% Example 3 40.6 22.8 43.8 Example 4 42.0 24.2 42.4 Example 5 38.5 23.6 38.7 Example 6 36.7 21.9 40.3 Comparative Example 1 41.8 30.7 26.6 Comparative Example 2 46.4 39.2 15.5 Comparative Example 3 37.7 25.8 31.6 Comparative Example 4 43.2 34.2 20.8 Comparative Example 5 45.4 40.7 10.4

[0071] It can be seen from Table 2 above that the shampoos prepared in Examples 3-6 of the present invention can effectively alleviate the symptoms of hair loss and have the efficacy of preventing and treating hair loss, and the effects are better than the shampoos prepared in Comparative Examples 1-5.

[0072] The above are only preferred embodiments of the present invention. It should be noted that the above preferred embodiments should not be regarded as limiting the present invention, and the protection scope of the present invention should be based on the scope defined by the claims. For ordinary technicians in this technical field, several improvements and modifications can be made without departing from the spirit and scope of the present invention, and these improvements and modifications should also be regarded as the protection scope of the present invention.

Claims

1. A shampoo for promoting hair growth and preventing hair loss, characterized in that: The invention is composed of the following raw materials in percentage by mass: 3-6% of apigenin composite nanoparticles, 2-4% of proanthocyanidin B2 liposomes, 10-15% of surfactants, 2-8% of moisturizing agents, 3-5% of thickeners, 0.05-0.1% of chelating agents, 0.5-1% of preservatives, 0.1-0.5% of flavors, and the balance is deionized water; the apigenin composite nanoparticles are Brazilian nut protein hydrolyzate nanoparticles loaded with apigenin, and the apigenin loading amount is 10-100 mg / g.

2. The shampoo according to claim 1, characterized in that The apigenin composite nanoparticles are prepared by the following method: Brazilian nut protein was dissolved in water to prepare a protein solution with a mass fraction of 7-8%, and then papain with a mass of 1-2.5% of Brazilian nut protein was added, and the solution was hydrolyzed at pH 6-7 and temperature 55-65°C for 2-3 h, the enzyme was inactivated, and the supernatant was obtained by centrifugation to obtain Brazilian nut protein hydrolyzate. The pH of the Brazilian nut protein hydrolyzate was adjusted to 10-12, apigenin was added under stirring conditions, and stirring was continued for 1-2 h, and the pH value of the mixed solution was adjusted to 6-7, the supernatant was obtained by centrifugation, and the supernatant was dried to obtain Brazilian nut protein hydrolyzate nanoparticles loaded with apigenin.

3. The shampoo according to claim 1, characterized in that The proanthocyanidin B2 liposome is prepared by the following method: S1. Mix hydrogenated lecithin, cholesterol and Tween 80 in a mass ratio of (8-9):(1-2):(1-2), add anhydrous ethanol to prepare a solution A with a concentration of 3-4 mg / mL; S2, dissolving proanthocyanidin B2 in a phosphate buffer solution with a pH of 5.0 to prepare a solution B with a concentration of 0.1-0.2 mg / mL; S3. Place solution A in a rotary evaporator to evaporate under reduced pressure to remove ethanol, then let it stand for 5-10 min, add solution B, and oscillate the hydration reaction in a 35-40°C water bath for 1-2 h to obtain a suspension, and ultrasonically oscillate the suspension in an ice bath for 10-30 min to obtain proanthocyanidin B2 liposomes.

4. The shampoo according to claim 1, characterized in that The surfactant includes one or more of sodium laureth sulfate, cocamidopropyl hydroxysulfobetaine, sodium methyl cocoyl taurate, cocamidopropyl betaine, and disodium cocamide glutamate.

5. The shampoo according to claim 1, characterized in that The moisturizing agent includes one or more of glycerin, butylene glycol, squalane, sodium hyaluronate, fructan and trehalose.

6. The shampoo according to claim 1, characterized in that The thickener includes one or more of hydroxyethyl cellulose, carbomer, acrylates / C10-30 alkyl acrylate cross-linked polymer, xanthan gum, ceteareth-60 myristyl glycol, and sodium acrylate / sodium acryloyldimethyl taurate copolymer.

7. The shampoo according to claim 1, characterized in that The chelating agent includes one or more of EDTA-2Na, tetrasodium glutamate diacetate, trisodium ethylenediamine disuccinate, and tetrasodium methylglycine diacetate.

8. The shampoo according to claim 1, characterized in that The preservative is a mixture of phenoxyethanol, ethylhexylglycerin, methylparaben or ethylparaben.

9. A method for preparing the shampoo according to any one of claims 1 to 8, characterized in that: The following steps are involved: Dissolve the surfactant, moisturizer, thickener and chelating agent in deionized water, heat to 80-85°C in a vacuum batching pot, keep warm and stir for 20-40 min, cool to 40-45°C, add apigenin composite nanoparticles, proanthocyanidin B2 liposomes, preservatives and flavors, stir evenly, adjust the pH to 6-7, and obtain the product.