A skin care composition containing microscopic algae extract and its preparation method and application

Abstract

The present invention relates to a skin care composition containing a microscopic chlorella extract, a preparation method and an application thereof, wherein the skin care composition containing a microscopic chlorella extract comprises a microscopic chlorella extract, seaweed polysaccharide and squalane. The present invention creatively finds that the microscopic chlorella extract is used in combination with seaweed polysaccharide and squalane, and has a synergistic effect in moisturizing, anti-aging, anti-oxidation, anti-inflammatory repair, and the combination of the three not only has more significant effects, but also better stability and higher safety.

Description

Technical Field

[0001] The invention belongs to the technical field of daily cosmetics, and in particular relates to a skin care composition containing an extract of Chlorella microcarpa, and a preparation method and application thereof. Background Art

[0002] With the development of the economy and the improvement of people's awareness of the pursuit of beauty, people take care of their skin more frequently, and the pursuit of beauty has gradually strengthened from "self-pleasing consumption" to "urgent need consumption". However, with the development of science and technology, the quality of various skin care products is uneven, and cosmetics with anti-aging, moisturizing, whitening, anti-inflammatory and repair series are also emerging in an endless stream.

[0003] CN117338626A discloses an anti-aging, whitening and moisturizing composition and a preparation method thereof. The composition comprises the following steps: breaking the wall of dendrobium officinale, oats and seabuckthorn, performing enzymolysis, filtering, retaining the solid, adding zinc salt and calcium salt to the filtrate to obtain a protein peptide-amino acid chelated Zn-Ca complex; mixing the solid with tremella and ginseng, extracting with boiling water, filtering to obtain a first solid, and precipitating with alcohol to obtain a composite polysaccharide; extracting liquorice with supercritical fluid to obtain liquorice essential oil; mixing the solid with the first solid, inoculating fermentation bacteria for fermentation, filtering, extracting and purifying the filtrate, and drying to obtain an active extract, which is mixed with the protein peptide-amino acid chelated Zn-Ca complex, the composite polysaccharide, the liquorice essential oil, sodium hyaluronate, niacinamide and a peptide to obtain an anti-aging, whitening and moisturizing composition, which has good antioxidant, whitening, anti-inflammatory and anti-allergic effects, can repair damaged skin barriers and has broad application prospects.

[0004] CN116327633A discloses a wrinkle-removing and anti-aging composition, including vitamin C or its derivatives and nicotinamide mononucleotide, or vitamin C or its derivatives and borneol, or nicotinamide mononucleotide and borneol, or vitamin C or its derivatives, nicotinamide mononucleotide and borneol. Or the above composition also includes taurine. The invention study shows that the wrinkle-removing and anti-aging composition can effectively remove senescent cells and promote skin wrinkle repair at a certain dose, and has an anti-aging effect that is significantly better than that of a single component. Animal experimental results also show that the combined compound can reduce the damage to the back skin of mice, reduce skin aging and inflammation, increase the synthesis of collagen fibers, and restore UVB-induced epidermal thickening.

[0005] However, although the above-mentioned cosmetics have added more active ingredients, it is difficult for the active ingredients to truly penetrate deep into the skin to exert their effects, and they have disadvantages such as low safety and poor stability. Based on this, it is of great significance to develop a skin care product that has multiple functions, high safety and good stability. Summary of the invention

[0006] In view of the deficiencies in the prior art, the object of the present invention is to provide a skin care composition containing a microscopic Chlorella extract and a preparation method and application thereof. The composition of the present invention has moisturizing, repairing, anti-oxidation, anti-aging and other effects, and has not only better effects but also better stability and higher safety.

[0007] In order to achieve the purpose of the invention, the present invention adopts the following technical solutions:

[0008] In a first aspect, the present invention provides a skin care composition containing a Chlorella minutissima extract, wherein the skin care composition containing a Chlorella minutissima extract comprises a Chlorella minutissima extract, seaweed polysaccharide and squalane.

[0009] Chlorella minutissima is a green algae belonging to the microalgae family. It is rich in protein, vitamins, minerals and other biochemical components that are beneficial to health. The present invention creatively discovered that when used in combination with seaweed polysaccharides and squalane, it has a synergistic effect in moisturizing, anti-aging, anti-oxidation and anti-inflammatory repair. The combination of the three can help the skin maintain moisture and make the skin look more moisturized; the powerful antioxidant effect of the three can slow down the skin aging process and reduce the appearance of fine lines and wrinkles; the growth factors and vitamins contained can promote the regeneration and repair of skin cells, and can help reduce redness, swelling and irritation.

[0010] Preferably, the skin care composition containing the Chlorella minutissima extract comprises 0.5-20 parts of Chlorella minutissima extract, 1-5 parts of seaweed polysaccharide and 1-10 parts of squalane by weight.

[0011] The amount of the micro-chlorella extract added in the skin care composition containing micro-chlorella extract of the present invention can be 0.8 parts, 1 parts, 2 parts, 5 parts, 7 parts, 9 parts, 10 parts, 13 parts, 15 parts, 17 parts or 19 parts, etc.;

[0012] The amount of seaweed polysaccharide added can be 1.2 parts, 1.5 parts, 1.8 parts, 2 parts, 2.2 parts, 2.5 parts, 2.8 parts, 3 parts, 3.2 parts, 3.5 parts, 3.8 parts, 4 parts, 4.2 parts, 4.5 parts or 4.8 parts, etc.;

[0013] The added amount of squalane can be 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts or 9 parts, etc.

[0014] Preferably, the skin care composition containing the Chlorella minuta extract comprises 3-18 parts of Chlorella minuta extract, 1.5-4 parts of seaweed polysaccharide and 2-9 parts of squalane by weight.

[0015] Preferably, the skin care composition containing the Chlorella minuta extract comprises 5-15 parts of Chlorella minuta extract, 2-4 parts of seaweed polysaccharide and 3-8 parts of squalane by weight.

[0016] Preferably, the skin care composition containing Chlorella minuta also includes other Chlorella extracts.

[0017] Preferably, the other coccus algae extracts include Nannochloropsis oculata extract and / or Haematococcus pluvialis extract.

[0018] Preferably, the skin care composition containing the microscopic Chlorella extract also includes 1-15 parts of other Chlorella extracts by weight, for example, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, 11 parts, 12 parts, 13 parts or 14 parts.

[0019] Preferably, the other sphaerocephala extracts include Nannochloropsis oculata extract and Haematococcus pluvialis extract.

[0020] Preferably, the mass ratio of the Nannochloropsis oculiformis extract to the Haematococcus pluvialis extract is 1:(0.5-10), for example, it can be 1:0.8, 1:1, 1:2, 1:3, 1:4, 1:5, 1:6, 1:7, 1:8 or 1:9, etc.

[0021] In the present invention, the skin care composition can make the effects of the composition in terms of moisturizing, anti-aging, anti-oxidation, anti-inflammatory and repair more significant by adding the above-mentioned specific ratio of Nannochloropsis oculi vein extract and Haematococcus pluvialis extract.

[0022] Preferably, the skin care composition containing the Chlorella minuta extract further comprises any one of an emulsifier, an emollient, a moisturizer or a preservative, or a combination of at least two thereof.

[0023] Preferably, the skin care composition containing the Chlorella miniata extract further comprises, by weight, 1-10 parts of an emulsifier, 1-10 parts of an emollient, 1-10 parts of a moisturizer and 0.1-1 parts of a preservative.

[0024] The amount of the emulsifier added to the skin care composition containing the microscopic chlorella extract of the present invention can be 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts or 9 parts, etc.;

[0025] The amount of the emollient added can be 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts or 9 parts, etc.;

[0026] The amount of the moisturizer added can be 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts or 9 parts, etc.;

[0027] The added amount of the preservative can be 0.2 parts, 0.3 parts, 0.4 parts, 0.5 parts, 0.6 parts, 0.7 parts, 0.8 parts or 0.9 parts, etc.

[0028] Preferably, the moisturizing agent includes any one of sodium hyaluronate, ceramide, butylene glycol or 1,2-hexanediol, or a combination of at least two thereof.

[0029] Preferably, the moisturizing agent comprises a combination of sodium hyaluronate, ceramide, butylene glycol and 1,2-hexanediol.

[0030] Preferably, the mass ratio of sodium hyaluronate, ceramide, butylene glycol and 1,2-hexanediol is (0.05-1):(0.1-5):(1-5):(0.5-1.5).

[0031] Wherein “0.05-1” can be 0.07, 0.09, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8 or 0.9, etc.;

[0032] “0.1-5” can mean 0.3, 0.5, 0.8, 1, 1.2, 1.5, 1.8, 2.2, 2.5, 2.8, 3, 3.2, 3.5, 3.8, 4, 4.2, 4.5 or 4.8, etc.;

[0033] “1-5” can be 1.2, 1.5, 1.8, 2.2, 2.5, 2.8, 3, 3.2, 3.5, 3.8, 4, 4.2, 4.5 or 4.8, etc.;

[0034] “0.5-1.5” can mean 0.6, 0.7, 0.8, 0.9, 1, 1.1, 1.2, 1.3 or 1.4, etc.

[0035] Preferably, the emulsifier comprises C12-20 acid PEG-8 ester and / or potassium cetyl phosphate.

[0036] Preferably, the emulsifier is a combination of C12-20 acid PEG-8 ester and potassium cetyl phosphate.

[0037] In the present invention, the emulsifier uses a combination of C12-20 acid PEG-8 ester and potassium cetyl phosphate, which can better emulsify the skin care composition and other components, making the stability of the system more excellent.

[0038] Preferably, the mass ratio of the C12-20 acid PEG-8 ester to potassium cetyl phosphate is 1:(0.1-1), for example, it can be 1:0.1, 1:0.2, 1:0.3, 1:0.4, 1:0.5, 1:0.6, 1:0.7, 1:0.8 or 1:0.9, etc.

[0039] Preferably, the emollient comprises isononyl isononanoate.

[0040] Preferably, the preservative comprises p-hydroxyacetophenone.

[0041] Other specific point values ​​within the above numerical ranges can be selected and will not be described in detail here.

[0042] In a second aspect, the present invention provides a method for preparing a skin care composition containing a microscopic chlorella extract as described in the first aspect, the preparation method comprising mixing and homogenizing the skin care composition containing a microscopic chlorella extract, microscopic chlorella extract, seaweed polysaccharide, and squalane in deionized water to obtain the skin care composition containing a microscopic chlorella extract.

[0043] Preferably, the preparation method comprises:

[0044] (1) mixing an emulsifier, a moisturizer, an emollient, a preservative, and deionized water, and subjecting the mixture to high-pressure microfluidization to obtain a vesicle solution;

[0045] (2) The vesicle solution obtained in step (1) is mixed with a microscopic Chlorella extract, seaweed polysaccharide, squalane, a microscopic Chlorella extract, and a microscopic Haematococcus pluvialis extract, and the mixture is treated with high-pressure microfluidization to obtain a skin care composition containing the microscopic Chlorella extract.

[0046] The preparation method of the present invention uses microencapsulation technology to encapsulate active ingredients to protect the active ingredients from damage by external factors, such as light, oxygen and pH changes, while promoting the penetration and absorption of these ingredients, thereby significantly improving the stability and bioavailability of the active ingredients in the formula, ensuring that they remain active when they reach the target skin layer.

[0047] Preferably, the mixing temperature in step (1) is 45-55°C, for example, 46°C, 48°C, 50°C, 52°C or 54°C.

[0048] Preferably, the pressure of the high-pressure microjet treatment in step (1) is 200-300 MPa, and the jet velocity is 500-700 m / s. For example, the pressure may be 210 MPa, 230 MPa, 250 MPa, 270 MPa or 290 MPa, etc.; the jet velocity may be 520 m / s, 550 m / s, 580 m / s, 600 m / s, 620 m / s, 650 m / s or 680 m / s, etc.

[0049] Preferably, the mixing temperature in step (2) is 40-50°C, for example, 41°C, 43°C, 45°C, 47°C or 49°C.

[0050] Preferably, in step (2), the pressure of the high-pressure microjet treatment in step (1) is 200-300 MPa, and the jet velocity is 500-700 m / s, for example, the pressure may be 210 MPa, 230 MPa, 250 MPa, 270 MPa or 290 MPa, etc.; the jet velocity may be 520 m / s, 550 m / s, 580 m / s, 600 m / s, 620 m / s, 650 m / s or 680 m / s, etc.

[0051] Other specific point values ​​within the above numerical ranges can be selected and will not be described in detail here.

[0052] In a third aspect, the present invention provides a use of the skin care composition containing the microcystis extract as described in the first aspect in the preparation of cosmetics.

[0053] Preferably, the cosmetics include essence, lotion, emulsion, cream, facial cleanser or facial mask.

[0054] Preferably, the mass percentage of the skin care composition containing the microscopic Chlorella extract in the cosmetic is 0.1-15%, for example, it can be 0.5%, 0.8%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13% or 14%, etc.

[0055] Other specific point values ​​within the above numerical ranges can be selected and will not be described in detail here.

[0056] Compared with the prior art, the present invention has the following beneficial effects:

[0057] (1) The present invention creatively discovered that the combination of seaweed polysaccharide and squalane has a synergistic effect in moisturizing, anti-aging, anti-oxidation, and anti-inflammatory repair. The combination of the three can help the skin maintain moisture and make the skin look more moisturized; the powerful antioxidant effect of the three can slow down the skin aging process and reduce the appearance of fine lines and wrinkles; the growth factors and vitamins contained can promote the regeneration and repair of skin cells, and can help reduce redness, swelling and irritation.

[0058] (2) The method for preparing the skin care composition containing the microscopic Chlorella extract provided by the present invention has simple operation steps, low cost, and is easy to industrialize and mass produce. DETAILED DESCRIPTION

[0059] In order to further explain the technical means and effects adopted by the present invention, the technical solution of the present invention is further described below in conjunction with the preferred embodiments of the present invention, but the present invention is not limited to the scope of the embodiments.

[0060] The following materials and comparative examples are as follows:

[0061]

[0062] The rest of the raw materials can be used as long as they are purchased from regular dealers.

[0063] Examples 1-3

[0064] This embodiment provides three skin care compositions containing microscopic chlorella extract. The formula and dosage of the hair mask with repairing and softening effects are shown in the following table. The dosage units of each component in the table are all "parts";

[0065]

[0066] The preparation method of the skin care composition containing the microscopic chlorella extract is as follows:

[0067] (1) mixing an emulsifier, a moisturizer, an emollient, a preservative, and deionized water at 50° C., and then subjecting the mixture to a high-pressure microfluidization treatment to obtain a vesicle solution, wherein the pressure of the high-pressure microfluidization treatment is 200 MPa and the jet velocity is 600 m / s;

[0068] (2) The vesicle solution obtained in step (1) is mixed with a microscopic Chlorella extract, seaweed polysaccharides, squalane, a microscopic Chlorella extract, and a microscopic Haematococcus pluvialis extract at 45° C., and then subjected to high-pressure microfluidization treatment to obtain the skin care composition containing the microscopic Chlorella extract. The pressure of the high-pressure microfluidization treatment is 200 MPa and the jet velocity is 600 m / s.

[0069] Example 4

[0070] This embodiment provides a skin care composition containing a microscopic Chlorella extract. The skin care composition containing a microscopic Chlorella extract is different from that of Example 1 only in that the formula does not include Haematococcus pluvialis extract and Nannochloropsis oculata extract, and the reduced amounts thereof are distributed to squalane, seaweed polysaccharides, and microscopic Chlorella extract in proportion to the number of parts. The remaining components and addition amounts are consistent with those of Example 1, and the preparation method refers to Example 1.

[0071] Example 5

[0072] This embodiment provides a skin care composition containing a microscopic Chlorella extract. The skin care composition containing a microscopic Chlorella extract is different from that in Example 1 only in that the formula does not include a Haematococcus pluvialis extract, and the reduced amount is supplemented by a Nannochloropsis oculi extract. The remaining components and addition amounts are consistent with those in Example 1. The preparation method refers to Example 1.

[0073] Example 6

[0074] This embodiment provides a skin care composition containing a microscopic Chlorella extract. The skin care composition containing a microscopic Chlorella extract is different from that in Example 1 only in that the formula does not include a Nannochloropsis oculata extract, and the reduced amount is supplemented by a Haematococcus pluvialis extract. The remaining components and addition amounts are consistent with those in Example 1. The preparation method refers to Example 1.

[0075] Example 7

[0076] This embodiment provides a skin care composition containing a microscopic chlorella extract. The skin care composition containing a microscopic chlorella extract is different from that in Example 1 only in that the formula does not include C12-20 acid PEG-8 ester, and the reduced amount is supplemented by potassium cetyl phosphate. The remaining components and addition amounts are consistent with those in Example 1. The preparation method refers to Example 1.

[0077] Example 8

[0078] This embodiment provides a skin care composition containing a microscopic chlorella extract. The skin care composition containing a microscopic chlorella extract is different from that in Example 1 only in that potassium cetyl phosphate is not included in the formula, and the reduced amount is supplemented by C12-20 acid PEG-8 ester. The remaining components and addition amounts are consistent with those in Example 1. The preparation method refers to Example 1.

[0079] Example 9

[0080] This embodiment provides a skin care composition containing a microscopic chlorella extract. The skin care composition containing a microscopic chlorella extract is different from that in Example 1 only in that sodium hyaluronate is not included in the formula, and the reduced amount thereof is distributed to ceramide, 1,2-hexanediol and butylene glycol in proportion to the number of parts. The remaining components and addition amounts are consistent with those in Example 1. The preparation method refers to Example 1.

[0081] Example 10

[0082] This embodiment provides a skin care composition containing a microscopic chlorella extract. The skin care composition containing a microscopic chlorella extract is different from that in Example 1 only in that ceramide is not included in the formula, and the reduced amount thereof is distributed to sodium hyaluronate, 1,2-hexanediol and butylene glycol in proportion to the number of parts. The remaining components and addition amounts are consistent with those in Example 1. The preparation method refers to Example 1.

[0083] Embodiment 11

[0084] This embodiment provides a skin care composition containing a microscopic chlorella extract. The skin care composition containing a microscopic chlorella extract is different from that in Example 1 only in that 1,2-hexanediol is not included in the formula, and the reduced amount thereof is distributed to sodium hyaluronate, ceramide and butylene glycol in proportion to the number of parts. The remaining components and addition amounts are consistent with those in Example 1. The preparation method refers to Example 1.

[0085] Example 12

[0086] This embodiment provides a skin care composition containing a microscopic chlorella extract. The skin care composition containing a microscopic chlorella extract is different from that in Example 1 only in that butylene glycol is not included in the formula, and the reduced amount thereof is distributed to sodium hyaluronate, ceramide and 1,2-hexanediol in proportion to the number of parts. The remaining components and addition amounts are consistent with those in Example 1. The preparation method refers to Example 1.

[0087] Comparative Example 1

[0088] This comparative example provides a skin care composition containing a microscopic chlorella extract. The skin care composition containing a microscopic chlorella extract is different from that in Example 1 only in that squalane is not included in the formula, and the reduced amount thereof is distributed to the seaweed polysaccharide and the microscopic chlorella extract in proportion to the number of parts. The remaining components and addition amounts are consistent with those in Example 1, and the preparation method refers to Example 1.

[0089] Comparative Example 2

[0090] This comparative example provides a skin care composition containing a microscopic chlorella extract. The skin care composition containing a microscopic chlorella extract is different from that in Example 1 only in that the formula does not include seaweed polysaccharides, and the reduced amount thereof is distributed to squalane and microscopic chlorella extract in proportion to the number of parts. The remaining components and addition amounts are consistent with those in Example 1, and the preparation method refers to Example 1.

[0091] Comparative Example 3

[0092] This comparative example provides a skin care composition, which differs from Example 1 only in that the formula does not include microscopic Chlorella extract, and the reduced amount is distributed to seaweed polysaccharides and squalane in proportion. The remaining components and addition amounts are consistent with Example 1, and the preparation method refers to Example 1.

[0093] Test Example 1 Anti-aging efficacy test

[0094] Test samples: The compositions provided in Examples 1-12 and the compositions provided in Comparative Examples 1-3 were added into deionized water respectively and diluted to a concentration of 10%.

[0095] The specific test method is as follows:

[0096] (1) Inhibition rate of metalloproteinase (MMP-1) expression:

[0097] Test principle: One of the main causes of skin aging is the change in the structure of the dermis. Due to the action of some external factors, the matrix metalloproteinases (MMPs) in the body are activated, which can lead to excessive degradation of collagen and elastin in the dermis that support the skin structure, resulting in aging symptoms such as wrinkles and inelasticity. Metalloproteinase MMP-1, also known as collagenase-1, can degrade collagen gelatin and proteoglycans of types I, II, III, VII and X. The anti-aging effect of these samples is evaluated by testing the inhibition rate of the samples on MMP-1;

[0098] Test method: Fibroblasts were seeded into 12-well cell culture plates, each well containing 0.75×10 5 cells and starved them in serum-free medium for 24 h. The starved cells were rinsed with PBS and treated with blue light. Then, the test sample was added to the cells twice within 48 h. The MMP-1 isolated in the culture medium was measured using a kit (BIOTRAK, RPN2610). The strength of the anti-aging effect was evaluated by calculating the expression inhibition rate of MMP-1;

[0099] The calculation formula is as follows: inhibition rate = (M0-M1) / M0×100%;

[0100] M0 is the expression level of MMP-1 without adding the test sample but after irradiation with blue light, and M1 is the expression level of MMP-1 after adding the test sample and irradiation with blue light;

[0101] (2) Determination of the inhibitory effect of elastase activity

[0102] Test principle: One of the main causes of skin aging is the change in the structure of the dermis. Due to the action of some external factors, the elastase in the body is activated, which can lead to excessive degradation of collagen and elastin in the dermis that support the skin structure. The anti-aging effect of these samples is evaluated by testing the inhibition rate of elastase on the samples;

[0103] Test method: 200 μL of each test sample and 50 μL of 20 μg / mL elastase-III solution were mixed in a 96-well plate in a 10 mg / L Tris hydrochloride buffer. 250 μM EGCG was used as a positive control group, and distilled water was used as a negative control group. The reaction was carried out at 25°C for 15 minutes. After the reaction, the absorbance at a wavelength of 415 nm was measured.

[0104] The calculation formula is as follows: Elastase activity inhibition rate (%) = 1-(CD) / (AB) × 100%;

[0105] A is the absorbance at 415 nm when no test substance is added but enzyme is added, B is the absorbance at 415 nm when no test substance and enzyme are added, C is the absorbance at 415 nm when test substance and enzyme are added, and D is the absorbance at 415 nm when test substance is added but enzyme is not added;

[0106] The specific test results are shown in Table 1 below:

[0107] Table 1

[0108]

[0109]

[0110] As can be seen from Table 1, the skin care compositions prepared in Examples 1-3 have an inhibition rate of more than 69% on the expression of metalloproteinase MMP-1 and an inhibition rate of more than 85% on elastase. Among them, the effect of Example 1 is higher than that of Comparative Examples 1-3, indicating that the formula of the composition provided by the present invention is not obvious, the microscopic algae extract, seaweed polysaccharide, and squalane complement each other and have a synergistic effect, and the three cooperate to achieve a more excellent anti-aging effect; at the same time, the inhibition rate of Example 1 is higher than that of Examples 4-6, indicating that the addition of other algae extracts and the selection of species also affect the anti-aging effect of the composition.

[0111] Test Example 2: Red blood cell hemolysis test

[0112] The red blood cell haemolysis test (RBC) is one of the alternative methods to the rabbit eye irritation test. The basic principle is to evaluate the damage of chemicals to eye tissue cells by measuring the amount of cell hemoglobin dissolution and the degree of denaturation. The RBC test can be used to evaluate the eye irritation of cosmetics.

[0113] The samples of Examples 1-12 were subjected to red blood cell hemolysis experiments. The experiments adopted the RBC experimental test method and judgment criteria of the European Alternative Method Validation Center. The specific judgment criteria are shown in Table 2, where HD 50 is the sample concentration when 50% of red blood cells are hemolyzed, DI is the protein denaturation index, and L / D is HD 50 Ratio to DI.

[0114] Table 2

[0115]

[0116]

[0117] The irritation results of the tested samples prepared in Examples 1-12 are shown in Table 3.

[0118] Table 3

[0119] sample L / D Grading Example 1 ＞100 Non-irritating Example 2 ＞100 Non-irritating Example 3 ＞100 Non-irritating Example 4 ＞100 Non-irritating Example 5 ＞100 Non-irritating Example 6 ＞100 Non-irritating Example 7 ＞100 Non-irritating Example 8 ＞100 Non-irritating Example 9 ＞100 Non-irritating Example 10 ＞100 Non-irritating Embodiment 11 ＞100 Non-irritating Example 12 ＞100 Non-irritating

[0120] From the sample irritation results in Table 3, it can be seen that the irritation classification of the compositions prepared in Examples 1 to 12 of the present invention is non-irritating, which shows that the skin care compositions of the present invention are mild and non-irritating.

[0121] Experimental Example 3 Study on Antioxidant Efficacy - DPPH Free Radical Scavenging Experiment

[0122] Take 1g of the sample of Example 1-6 and Comparative Example 1-3, add 2mL of anhydrous ethanol, add 2mL of 60μmol / L DPPH solution after complete dissolution, mix, let stand for 30min, adjust to zero with anhydrous ethanol, and measure the absorbance at 517nm as Ai; take 2mL of anhydrous ethanol solvent + 2mL DPPH solution and mix to determine the absorbance extreme Ac in the same way; take 1g of the matrix of the composition and dissolve it, add 2mL of anhydrous ethanol, then add 2mL of anhydrous ethanol solvent and mix to determine the absorbance as Aj.

[0123] The free radical scavenging rate was calculated according to the following formula.

[0124] Clearance (%) = [1-(Ai-Aj) / Ac] × 100%

[0125] Among them, Aj is the contribution of the reaction sample itself to the absorbance; Ai is the absorbance value of the sample after the action of DPPH; Ac is the absorption of DPPH itself at the measurement wavelength.

[0126] The DPPH free radical scavenging rate reflects its antioxidant capacity to a certain extent. The higher the scavenging rate, the better the antioxidant degree and the better the anti-aging effect. Therefore, the anti-aging effect of the composition can be judged by studying its ability to scavenge DPPH free radicals. The results are shown in Table 4 (each group of samples was measured 5 times in parallel, and the data are presented as average values).

[0127] Table 4

[0128] Sample name DPPH clearance rate (%) Example 1 88.75 Example 2 89.28 Example 3 87.54 Example 4 77.18 Example 5 83.41 Example 6 81.76 Comparative Example 1 79.32 Comparative Example 2 78.45 Comparative Example 3 76.92

[0129] As shown in Table 4, the combination prepared in Examples 1-3 has a good scavenging effect on DPPH, with a scavenging rate of more than 86%. From the comparison between Example 1 and Comparative Examples 1-3, it can be seen that the microscopic Chlorella extract, seaweed polysaccharide and squalane in the skin care composition of the present invention have a synergistic effect in antioxidant efficacy, and the synergistic combination of the three can make the product's antioxidant anti-aging efficacy more excellent.

[0130] Test Example 4 Anti-inflammatory Efficacy Test

[0131] Test samples: samples provided in Examples 1, 4-6, and Comparative Examples 1-3;

[0132] The test method is as follows:

[0133] (1) Dilute the Raw264.7 cells in good culture state to a concentration of 1×10 5 cfu / mL;

[0134] (2) Prepare a 12-well cell plate and add a concentration of 1×10 5 cfu / mL of Raw264.7 cells were transferred to a 12-well plate using a pipette, 1 mL was added to each well, and cultured at 37°C for 24 h;

[0135] (3) The experiment included 10 groups, blank group (no sample added), LPS group (1 μg / mL LPS added), test group (2 mg / mL samples provided in Examples 1, 4-6, and Comparative Examples 1-3 added with 1 μg / mL LPS), and positive control group (0.1 mg / mL dexamethasone added). The supernatant of the Raw264.7 cell culture fluid was discarded, and the corresponding solution of each group was added, and cultured at 37°C for 24 h;

[0136] (4) The supernatant was centrifuged and the concentrations of inflammatory factors TNF-α and IL-6 were detected according to the test steps on the ELISA kit. Each group was measured 5 times in parallel and the average value was taken.

[0137] The specific test results are shown in Table 5 below:

[0138] Table 5

[0139]

[0140]

[0141] As can be seen from the data in Table 5, the composition provided in Example 1 of the present invention has a very significant inhibitory effect on inflammatory factors TNF-α and IL-6, can effectively inhibit the release and transmission of inflammatory factors, can play an anti-inflammatory and soothing role, and thus effectively repair inflamed skin. Among them, the microscopic algae extract, seaweed polysaccharide, and squalane have a synergistic effect in inhibiting inflammatory factors. When other algae extracts are added at the same time, the product has a better inhibitory effect on inflammatory factors.

[0142] Test Example 5 Evaluation of Moisturizing Effect

[0143] The samples obtained in Example 1, Examples 9-12 and Comparative Examples 1-3 were evaluated for their long-lasting moisturizing effect and skin tightening effect:

[0144] 80 trial subjects aged 20-45 were randomly selected and divided into 8 groups, with 10 in each group. The 8 groups of trial subjects used the samples obtained in Example 1, Examples 9-12 and Comparative Examples 1-3, respectively, once in the morning and evening after cleansing, with the same usage amount, for 30 days, and no other skin care products were used during this period.

[0145] The skin moisture content was tested with a skin moisture tester Corneometer CM825 on the 0th day and the 30th day, respectively, to evaluate the skin moisture content before and after using the sample. The skin moisture content change rate = (skin moisture content after 30 days of use - skin moisture content before use) / skin moisture content before use × 100%; the test results are shown in Table 6.

[0146] Table 6

[0147]

[0148]

[0149] As shown in Table 6, the skin care composition and lotion of the present invention have good long-lasting moisturizing effect, wherein the microscopic chlorella extract, seaweed polysaccharide, and squalane promote each other in this effect, and have an unexpected synergistic effect. The formula of the moisturizer also has an important influence on the moisturizing property of the product.

[0150] Test Example 6

[0151] The stability of the samples prepared in Examples 1-3 and Examples 7-8 was evaluated:

[0152] (1) Cold stability: Each group of samples was subjected to a cold test. The cold test was performed by placing the samples at -15°C for 24 hours and then returning them to room temperature (25°C) to observe the stability of the samples.

[0153] (2) Centrifugal stability: Each group of samples was subjected to a centrifugal test at 2000 r / min and room temperature (25°C) for 30 min, and the stability of the sample was observed;

[0154] (3) High temperature stability: Each group of samples was subjected to a high temperature test, and the samples were placed in a 48°C environment for 24 hours and then placed in a -10°C environment for 1 hour, and the stability of the samples was observed. The results are shown in Table 7.

[0155] Table 7

[0156] Group Cold resistance and stability Centrifugal stability High temperature stability Example 1 No precipitation No precipitation No precipitation Example 2 No precipitation No precipitation No precipitation Example 3 No precipitation No precipitation No precipitation Example 7 A small amount of precipitation No precipitation A small amount of precipitation Example 8 A small amount of precipitation No precipitation A small amount of precipitation

[0157] It can be seen from Table 7 that the samples involved in the present invention have good cold resistance stability, centrifugal stability and high temperature stability, among which the formula of the emulsifier has an impact on the stability of the product.

[0158] The applicant declares that the present invention uses the above-mentioned embodiments to illustrate a skin care composition containing a microscopic algae extract and its preparation method and application, but the present invention is not limited to the above-mentioned embodiments, that is, it does not mean that the present invention must rely on the above-mentioned embodiments to be implemented. Those skilled in the art should understand that any improvement of the present invention, equivalent replacement of various raw materials of the product of the present invention, addition of auxiliary ingredients, selection of specific methods, etc., all fall within the protection scope and disclosure scope of the present invention.

[0159] The preferred embodiments of the present invention are described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the technical concept of the present invention, a variety of simple modifications can be made to the technical solution of the present invention, and these simple modifications all belong to the protection scope of the present invention.

[0160] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not further describe various possible combinations.

Claims

1. A skin care composition containing a microscopic Chlorella extract, characterized in that: The skin care composition containing the microscopic chlorella extract comprises microscopic chlorella extract, seaweed polysaccharide, squalane, microscopic chlorella extract and haematococcus pluvialis extract; The skin care composition containing the microscopic chlorella extract comprises, by weight, 5-15 parts of microscopic chlorella extract, 1-5 parts of seaweed polysaccharide, 3-8 parts of squalane, 1-15 parts of Nannochloropsis oculi extract and Haematococcus pluvialis extract; The mass ratio of the Nannochloropsis oculiformis extract to the Haematococcus pluvialis extract is 1:(0.5-10).

2. The skin care composition containing the microscopic Chlorella extract according to claim 1, characterized in that: The skin care composition containing the microscopic Chlorella extract also includes any one of an emulsifier, a moisturizer, a humectant or a preservative, or a combination of at least two of them.

3. The skin care composition containing the microscopic Chlorella extract according to claim 2, characterized in that: The skin care composition containing the microscopic Chlorella extract further comprises, by weight, 1-10 parts of an emulsifier, 1-10 parts of a moisturizer, 1-10 parts of a humectant and 0.1-1 parts of a preservative.

4. The skin care composition containing the microscopic Chlorella extract according to claim 2 or 3, characterized in that: The moisturizing agent includes any one of sodium hyaluronate, ceramide, butylene glycol or 1,2-hexanediol, or a combination of at least two thereof.

5. The skin care composition containing the microscopic Chlorella extract according to claim 4, characterized in that: The moisturizer includes a combination of sodium hyaluronate, ceramide, butylene glycol and 1,2-hexanediol.

6. The skin care composition containing the microscopic Chlorella extract according to claim 2 or 3, characterized in that: The emulsifiers include C12-20 acid PEG-8 esters and / or potassium cetyl phosphate.

7. The skin care composition containing the microscopic Chlorella extract according to claim 6, characterized in that: The emulsifier is a combination of C12-20 acid PEG-8 ester and potassium cetyl phosphate.

8. The skin care composition containing the extract of Microcystis microcarpa according to claim 2 or 3, characterized in that: The emollient includes isononyl isononanoate.

9. The skin care composition containing the extract of Microcystis microcarpa according to claim 2 or 3, characterized in that: The preservative includes p-hydroxyacetophenone.

10. The method for preparing a skin care composition containing a microcystis extract according to any one of claims 1 to 9, characterized in that: The preparation method comprises the steps of homogenizing and mixing the microscopic chlorella extract, seaweed polysaccharide, squalane, microscopic chlorella extract and microscopic haematococcus pluvialis extract in deionized water to obtain the skin care composition containing the microscopic chlorella extract.

11. The method for preparing the skin care composition containing the extract of Chlorella microcarpa according to claim 10, characterized in that: The preparation method comprises: (1) mixing an emulsifier, a moisturizer, an emollient, a preservative, and deionized water, and subjecting the mixture to high-pressure microfluidization to obtain a vesicle solution; (2) The vesicle solution obtained in step (1) is mixed with a microscopic Chlorella extract, seaweed polysaccharide, squalane, a microscopic Chlorella extract, and a microscopic Haematococcus pluvialis extract, and the mixture is treated with high-pressure microfluidization to obtain a skin care composition containing the microscopic Chlorella extract.

12. The preparation method according to claim 11, characterized in that: The mixing temperature in step (1) is 45-55°C.

13. The preparation method according to claim 11, characterized in that: The pressure of the high-pressure microfluidization treatment in step (1) is 200-300 MPa, and the jet velocity is 500-700 m / s.

14. The preparation method according to claim 11, characterized in that: The mixing temperature in step (2) is 40-50°C.

15. The preparation method according to claim 11, characterized in that: The pressure of the high-pressure microfluidization treatment in step (2) is 200-300 MPa, and the jet velocity is 500-700 m / s.

16. Use of the skin care composition containing the Chlorella minutissima extract according to any one of claims 1 to 9 in the preparation of cosmetics.

17. Use of the skin care composition containing the microcystis extract according to claim 16 in preparing cosmetics, characterized in that: The cosmetics include essence, lotion, emulsion, cream, facial cleanser or facial mask.

18. Use of the skin care composition containing the extract of Chlorella microcarpa as claimed in claim 17 in the preparation of cosmetics, characterized in that: The mass percentage of the skin care composition containing the microscopic Chlorella extract in the cosmetics is 0.1-15%.

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