A process for extracting turmeric wall-broken ganoderma lucidum spore powder

Abstract

The invention discloses a turmeric wall-breaking ganoderma lucidum spore powder extraction process, which specifically relates to the field of biological extraction technology. The process includes ultrasonic cleaning equipment, drying equipment, freezing grinding machine, ultrasonic wall-breaking machine, stirrer, supercritical CO2 extraction device, ceramic membrane filter, high-speed centrifuge, molecular distillation equipment and vacuum freeze dryer; the high process specifically includes S1, raw material processing, S2, wall-breaking processing, S3, mixed extraction, S4, filtration separation, S5, concentration and drying. The invention adopts a series of fine process flows to achieve efficient extraction and purification of active ingredients in turmeric root and ganoderma lucidum spore powder, and improve the extraction effect.

Description

Technical Field

[0001] The invention relates to the technical field of biological extraction, and more specifically to a process for extracting turmeric wall-broken ganoderma lucidum spore powder. Background Art

[0002] Turmeric is a traditional Chinese medicinal material, which is the dried rhizome of the ginger plant turmeric. It has the effects of promoting qi and removing blood stasis, relieving menstruation and relieving pain.

[0003] Ganoderma spores are extremely tiny oval reproductive cells that are ejected from the gills of Ganoderma during its growth and maturity period. They have the functions of enhancing the body's immunity, inhibiting tumors, protecting against liver damage, and providing radiation protection.

[0004] At present, in the traditional herbal extraction process, especially for natural materials rich in bioactive ingredients such as turmeric root and Ganoderma lucidum spore powder, the efficiency of the extraction process and the quality of the product are the key factors to measure the success of the technology. Since the active ingredients in these plant materials are usually wrapped in hard cell walls, traditional extraction methods often have difficulty in efficiently releasing these active ingredients, and their biological activity is easily damaged by high temperature or chemical treatment.

[0005] After searching, a Chinese patent with publication number CN111904987A discloses a processing technology for broken wall Ganoderma lucidum spore powder. The process softens the spore powder raw material by boiling and then repeatedly freezes it with liquid nitrogen. The spore wall can be processed by the rapid thermal contraction and then ultrasonically treated by mixing with sodium chloride particles, which is convenient for filtration and removal to avoid mixing. At the same time, the wall breaking rate and processing efficiency are effectively improved, the product quality is guaranteed, the processing is convenient, and it is conducive to promotion and use. Although the spore powder is easy to be broken after repeated high-temperature boiling, the biologically active substances inside it will be lost in large quantities, which ultimately leads to low purity of the spore powder.

[0006] After searching, a Chinese patent with publication number CN108888638A discloses a processing technology for wall-broken Ganoderma lucidum spore powder. The method adopts the synergistic effect of ultrasound, freezing, enzyme treatment and wall-breaking treatment. Impurities in the Ganoderma lucidum spore powder are effectively removed through deionized water washing, coarse crushing, ultrasound treatment and enzyme treatment. The addition of wall-lytic enzyme effectively reduces the difficulty of dissolution. The wall-breaking of the Ganoderma lucidum spore powder is completed by using ultra-low temperature freezing and air flow wall-breaking dual technology. The process operation is simple and the preparation cost is low. It effectively solves the problems of incomplete wall-breaking of the existing Ganoderma lucidum spore powder, incomplete retention of the nutritional components of the Ganoderma lucidum spore powder, and easy destruction of its nutritional components. However, the purity of the spore powder of this process is not high. After screening after the wall-breaking, certain non-target components will still exist inside it, affecting the purity of the final spore powder.

[0007] In view of the above situation, the present invention proposes a turmeric wall-broken Ganoderma lucidum spore powder extraction process and equipment for extracting the spore powder, which realizes the efficient extraction and purification of active ingredients in turmeric root and Ganoderma lucidum spore powder. Summary of the invention

[0008] In order to overcome the above defects of the prior art, the present invention provides a process for extracting turmeric wall-broken Ganoderma lucidum spore powder to solve the problems raised in the above background technology.

[0009] To achieve the above-mentioned purpose, the present invention provides the following technical scheme: a process for extracting turmeric-wall-broken Ganoderma lucidum spore powder, which comprises an ultrasonic cleaning device, a drying device is arranged on one side of the ultrasonic cleaning device, a freezing grinder is arranged on one side of the drying device, an ultrasonic wall-breaking machine is arranged on one side of the freezing grinder, a stirrer is arranged on one side of the ultrasonic wall-breaking machine, a supercritical CO2 extraction device is arranged on one side of the stirrer, a ceramic membrane filter is arranged on one side of the supercritical CO2 extraction device, a high-speed centrifuge is arranged on one side of the ceramic membrane filter, a molecular distillation device is arranged on one side of the high-speed centrifuge, and a vacuum freeze dryer is arranged on one side of the molecular distillation device.

[0010] Preferably, the process is prepared using the above-mentioned equipment, and specifically comprises the following steps:

[0011] S1, raw material processing, fresh turmeric root and ganoderma lucidum spore powder are cleaned by ultrasonic cleaning equipment to remove impurities inside, and then taken out and dried by drying equipment;

[0012] S2, wall breaking treatment, using a freezing grinder and an ultrasonic wall breaking machine to break the dried turmeric root and ganoderma lucidum spore powder respectively, to release the active ingredients in the turmeric root and ganoderma lucidum spore powder;

[0013] S3, mixed extraction, adding the broken turmeric root and ganoderma lucidum spore powder into a blender, adding 10% of the total content of turmeric root and ganoderma lucidum spore powder, enzyme and ethanol, as well as polysorbate surfactant and polyamide dispersant to mix, to obtain a mixture, adding the mixture into a supercritical CO2 extraction device, and extracting the mixture using supercritical CO2 extraction technology to dissolve the active ingredients;

[0014] S4, filtering and separating, adding the mixture extracted in S3 to a ceramic membrane filter for preliminary filtration to remove solid residues, and centrifuging the filtered mixture using a high-speed centrifuge to separate the active ingredients, adding phosphate buffer for washing to remove non-target components;

[0015] S5, concentration and drying, adding the mixture filtered and separated in S4 to a molecular distillation device, using molecular distillation technology to concentrate the liquid after centrifugation to remove the solvent, and using a vacuum freeze dryer to dry the concentrated liquid to obtain the final extract powder.

[0016] Preferably, in S1, when the turmeric root and the ganoderma lucidum spore powder are cleaned by an ultrasonic cleaning device, the frequency of the ultrasonic cleaning device is 25-40KHz and the power is 0.3-0.8W / cm 2 , cleaning temperature 30-50℃, cleaning time 10-30min;

[0017] When the cleaned turmeric root and ganoderma lucidum spore powder are dried by drying equipment, the drying temperature is 40-60° C., the drying time is 2-6 hours, and the drying is uniform.

[0018] Preferably, in S2, when a freezing grinder and an ultrasonic wall-breaking machine are used to break the dried turmeric root and Ganoderma lucidum spore powder, respectively, the temperature of the freezing grinder is -50°C to 0°C, the grinding speed is 800-1600r / min, and the grinding time is 8-15min;

[0019] The frequency of ultrasonic wall breaking machine is 20-40KHz, and the power is 0.3-1.0W / cm 2 , time is 8-15min.

[0020] Preferably, in S3, the broken turmeric root and ganoderma spore powder are added to a stirrer, and when 10% of the total content of enzyme and ethanol of turmeric root and ganoderma spore powder, as well as polysorbate surfactant and polyamide dispersant are added for mixing, the stirring speed of the stirrer is 80-180r / min, the time is 6-17min, and the temperature is 45-50°C.

[0021] Preferably, in S3, the mixture is added to a supercritical CO2 extraction device. When the mixture is extracted using supercritical CO2 extraction technology, the pressure of the supercritical CO2 extraction device is 100-300 bar, the temperature is 35-40°C, the time is 15-35 min, and the CO2 flow rate is 0-5 L / h.

[0022] Preferably, in S4, when the mixture extracted in S3 is added to a ceramic membrane filter for preliminary filtration, the pore size of the ceramic membrane filter is 0.1-0.5 μm, the temperature is 20-25° C., and the pressure is 5-20 bar;

[0023] When the filtered mixture is centrifuged in a high-speed centrifuge to separate the active ingredient, the speed of the high-speed centrifuge is 5000-12000 r / min, the time is 5-20 min, the temperature is 4-22° C., and the relative centrifugal force is 500-1500 g.

[0024] Preferably, in S5, the mixture filtered and separated in S4 is added to a molecular distillation device, and when the liquid after centrifugation is concentrated by molecular distillation technology, the heating temperature is 40-80°C, the pressure is 1-50mbar, and the condensation temperature is 10-20°C;

[0025] When using a vacuum freeze dryer to dry the concentrated liquid, it is divided into three stages, including;

[0026] The pre-freeze drying temperature is -40 to -60°C, the time is 4-6 hours, and the pressure is 10-30 mbar;

[0027] The primary drying temperature is -20 to 20°C, the time is 18-48h, and the pressure is 10-30mbar;

[0028] The secondary drying temperature is 20-40°C, the time is 5-28h, and the pressure is 10-30mbar.

[0029] Preferably, the above-mentioned equipment is also connected to a control system and a monitoring system for controlling each device, wherein the monitoring system uses sensors to monitor the key parameters of each step in real time, such as temperature, pressure and flow rate, and the control system controls the equipment to adjust parameters according to the sensor data.

[0030] Technical effects and advantages of the present invention:

[0031] 1. The present invention utilizes cryo-grinding and ultrasonic cell wall breaking technology to destroy the raw material cell structure, and combines a specific ratio of enzyme and ethanol for mixed extraction, as well as optimized stirring conditions, to ensure the full release of active ingredients and maximize the extraction efficiency;

[0032] 2. The present invention effectively avoids the loss of heat-sensitive substances and reduction of biological activity that may be caused by high temperature by adopting low temperature control during the extraction and concentration process, such as frozen grinding, molecular distillation and vacuum freeze drying;

[0033] 3. The present invention reduces the use of organic solvents by adopting supercritical CO2 extraction technology and molecular distillation, meets environmental protection requirements, and improves operational safety. In addition, impurities and non-target components are removed through precisely controlled ceramic membrane filtration and high-speed centrifugal separation steps, ensuring the high purity and quality consistency of the final product.

[0034] 4. The temperature, pressure, time and added chemical substances in the whole production process of the present invention are carefully selected and controlled to protect the biological activity of the extract and ensure the efficacy of the final product. In addition, the introduction of a control system and a real-time monitoring system makes the whole extraction process highly automated, improves production efficiency and the accuracy of process control, and ensures repeatability and stability between batches. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] Figure 1 It is a schematic diagram of the connection structure of various devices of the present invention.

[0036] Figure 2 It is a flow chart of the present invention.

[0037] The accompanying drawings are marked as follows: 1. Ultrasonic cleaning equipment; 2. Drying equipment; 3. Freeze grinder; 4. Ultrasonic wall breaking machine; 5. Agitator; 6. Supercritical CO2 extraction device; 7. Ceramic membrane filter; 8. High-speed centrifuge; 9. Molecular distillation equipment; 10. Vacuum freeze dryer; 11. Control system; 12. Monitoring system. DETAILED DESCRIPTION

[0038] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0039] Embodiment 1,

[0040] This embodiment provides a turmeric wall-broken Ganoderma lucidum spore powder extraction process, which specifically involves multiple equipment, including an ultrasonic cleaning device 1, a drying device 2, a freezing grinder 3, an ultrasonic wall-breaking machine 4, a stirrer 5, a supercritical CO2 extraction device 6, a ceramic membrane filter 7, a molecular distillation device 9 and a vacuum freeze dryer 10.

[0041] Specifically, the above-mentioned equipment is also connected to a control system 11 and a monitoring system 12 for controlling each device, wherein the monitoring system 12 uses sensors to monitor the key parameters of each step in real time, such as temperature, pressure and flow rate, and the control system 11 controls the equipment to adjust parameters according to the sensor data.

[0042] Specifically, when the above equipment is used to extract the turmeric root and ganoderma lucidum spore powder, first, the ultrasonic cleaning equipment 1 uses high-frequency ultrasonic waves to produce cavitation effect in the liquid to remove impurities on the surface and inside of the turmeric root and ganoderma lucidum spore powder. The drying equipment 2 uses hot air or other heating methods to remove moisture from the raw materials to create suitable conditions for subsequent processing. The freezing grinder 3 grinds the material into fine particles under low temperature conditions by mechanical force to destroy the cell wall and release the active ingredients. The ultrasonic wall breaker 4 uses the energy of ultrasonic waves to destroy the cell structure of the raw material and promote the release of the active ingredients. The agitator 5 can mix the raw materials with additives (such as enzymes, ethanol, surfactants, dispersants, etc.) to ensure that the reaction proceeds evenly. row, supercritical CO2 extraction device 6, using supercritical CO2 as a solvent to extract the active ingredients in the mixture, because supercritical CO2 has excellent solubility and is easy to remove from the extract, ceramic membrane filter 7, preliminarily separating solids and liquids, removing large molecules and incompletely extracted residues, high-speed centrifuge 8, further separating solid and liquid components in the suspension through the centrifugal force generated by high-speed rotation, molecular distillation equipment 9, separating and concentrating target components according to the molecular evaporation and condensation characteristics of different substances, vacuum freeze dryer 10, directly hydrating the solvent, usually water, from a frozen state under vacuum and low temperature conditions to obtain a dry extract powder;

[0043] Furthermore, when the present invention extracts the turmeric wall-broken Ganoderma lucidum spore powder through the above-mentioned equipment, it integrates a variety of physical and chemical methods to effectively destroy the raw material cell wall and fully release the active ingredients, thereby improving the extraction rate and purity, and adopts low-temperature wall-breaking and freeze-drying technology to protect heat-sensitive substances from heat damage and retain more biological activity. The continuous production line and automated control system 11 reduce manual operation errors and improve production efficiency and product quality stability. Compared with traditional solvent extraction, supercritical CO2 extraction reduces the use of organic solvents and is more environmentally friendly and safe. Key parameters are monitored in real time by sensors, and the equipment operation status is automatically adjusted in combination with the control system 11, ensuring accurate control of the entire extraction process;

[0044] In summary, this technical solution realizes an efficient, accurate and environmentally friendly turmeric wall-broken Ganoderma lucidum spore powder extraction process by integrating multiple equipment such as ultrasonic cleaning, drying, cryogenic grinding, ultrasonic wall breaking, stirring and mixing, supercritical CO2 extraction, ceramic membrane filtration, high-speed centrifugation, molecular distillation and vacuum freeze drying, and assisted by a control system 11 and a real-time monitoring system 12. The working principle covers the process from preliminary cleaning of the raw materials to the acquisition of the final extract powder, involving multiple steps such as physical wall breaking, chemical extraction, solid-liquid separation, concentration and drying. During the whole process, key parameters such as temperature, pressure and flow rate are monitored and adjusted in real time to ensure the optimization of extraction efficiency and product quality. The expected effects include improving the extraction rate and purity of active ingredients, protecting heat-sensitive substances to retain biological activity, improving production efficiency and stability, and reducing the impact of organic solvent use on the environment. In short, this technical solution not only ensures product quality, but also takes into account the sustainability and economy of production.

[0045] Embodiment 2,

[0046] This embodiment provides a process for extracting turmeric wall-broken Ganoderma lucidum spore powder, which needs to use the equipment in Example 1 for extraction operation. Specifically, the process includes the following steps:

[0047] S1, raw material processing, fresh turmeric root and ganoderma lucidum spore powder are cleaned by ultrasonic cleaning equipment 1 to remove impurities inside, and then taken out and dried by drying equipment 2;

[0048] The ultrasonic cleaning equipment 1 has a frequency of 30KHz and a power of 0.5W / cm 2 , cleaning temperature is 35℃, cleaning time is 15min, drying temperature is 45℃, drying time is 3H, and drying is carried out evenly.

[0049] Specifically, in this step, the parameters of the ultrasonic cleaning equipment 1 are set to generate a cavitation effect when the raw material is cleaned, so that tiny bubbles are formed inside the liquid and quickly closed, thereby generating strong microjets and shear forces. These forces can destroy the contamination layer on the surface of the raw material and clean away the attached impurities. The parameters of the drying equipment 2 can evaporate the moisture of the raw material without damaging the heat-sensitive substances in the raw material. Therefore, this step not only removes impurities in the raw material through ultrasonic cleaning and drying treatment, but also provides the necessary preliminary preparation for effectively releasing and protecting the active ingredients during the extraction process.

[0050] S2, wall breaking treatment, using a freezing grinder 3 and an ultrasonic wall breaking machine 4 to break the dried turmeric root and ganoderma lucidum spore powder respectively, to release the active ingredients in the turmeric root and ganoderma lucidum spore powder;

[0051] The temperature of the freezing grinder 3 is -45°C, the grinding speed is 1000r / min, the grinding time is 10min, the frequency of the ultrasonic wall breaking machine 4 is 25KHz, and the power is 0.5W / cm 2 , time is 10 minutes.

[0052] Specifically, in this step, when the parameters of the cryo-grinder 3 are set to grind the turmeric root, the low temperature can reduce the thermal damage and oxidation of the material. At the same time, the mechanical action of grinding helps to break the cell wall and release the internal components. The ultrasonic wall-breaking machine 4 can break the wall of the Ganoderma lucidum spore powder to break the cell tissue. Then, the dual effects of cryo-grinding and ultrasonic wall-breaking are adopted to significantly improve the release rate of active ingredients from the raw materials, creating favorable conditions for the subsequent extraction process. Low-temperature grinding and precise ultrasonic treatment time and intensity reduce damage to heat-sensitive substances, help retain the functions of biologically active substances, and not only effectively extract the active ingredients in turmeric root and Ganoderma lucidum spore powder, but also provide the possibility of maximizing the protection of the biological activity of these ingredients.

[0053] S3, mixed extraction, adding the broken turmeric root and ganoderma lucidum spore powder into the agitator 5, adding 10% of the total content of turmeric root and ganoderma lucidum spore powder, enzyme and ethanol, as well as polysorbate surfactant and polyamide dispersant to mix, to obtain a mixture, adding the mixture into a supercritical CO2 extraction device, and extracting the mixture by supercritical CO2 extraction technology to dissolve the active ingredients;

[0054] Among them, the stirring speed of agitator 5 is 95r / min, the time is 10min, the temperature is 48°C, the pressure of the supercritical CO2 extraction device is 150bar, the temperature is 38°C, the time is 20min, and the CO2 flow rate is 1.5L / h.

[0055] Specifically, in this step, first, the raw material after the cell wall is broken is mixed with enzyme, ethanol, surfactant and dispersant. This process is completed by using a stirrer 5. The control of stirring speed and time is intended to ensure the uniformity and extraction efficiency of the mixture. The added enzyme can improve the extraction rate, and ethanol as a solvent is conducive to the dissolution of the active ingredient. The polysorbate surfactant and the polyamide dispersant help to improve the solubility and stability of the active ingredient in the mixture.

[0056] Then, the mixture is transferred to a supercritical CO2 extraction device 6, where supercritical CO2 is used as an extraction medium. Supercritical CO2 has unique physical and chemical properties, including high solubility and low viscosity, which enables it to effectively dissolve the target active ingredients and quickly deliver these ingredients, thereby improving the extraction efficiency and product purity;

[0057] In addition, the advantages of supercritical CO2 extraction technology include extraction at lower temperatures, usually at 35-40°C, which helps protect heat-sensitive substances from damage and avoids the environmental and health problems that may be caused by the use of organic solvents in traditional extraction methods;

[0058] In short, this step not only improves the extraction efficiency and product quality, but also ensures the safety and environmental protection of the extraction process through advanced mixed extraction and supercritical CO2 extraction technology.

[0059] S4, filtering and separating, adding the mixture extracted in S3 to a ceramic membrane filter 7 for preliminary filtration to remove solid residues, and centrifuging the filtered mixture using a high-speed centrifuge 8 to separate active ingredients, adding phosphate buffer for washing to remove non-target components;

[0060] The pore size of the ceramic membrane filter 7 is 0.2 μm, the temperature is 22° C., the pressure is 8 bar, the rotation speed of the high-speed centrifuge 8 is 8000 r / min, the time is 10 min, the temperature is 10° C., and the relative centrifugal force is 800 g.

[0061] Specifically, in this step, the ceramic membrane filter 7 is used to work under controlled temperature and pressure conditions, which can effectively remove large solid residues and undissolved impurities in the mixture, thereby improving the quality of the final product. The filtered mixture is centrifuged using the relative centrifugal force generated by the high-speed centrifuge 8, which can separate different components in the mixture according to the density difference of the substances, thereby separating the active ingredient from other small molecular impurities or suspended particles, and the non-target components are removed, while the active ingredient is retained in the solution. Washing with phosphate buffer can help maintain pH stability, protect the active ingredient from pH fluctuations, and help remove impurities that are unstable or insoluble at a specific pH.

[0062] In short, this step further removes impurities in the extract based on physical screening and centrifugal force, improves the purity of the active ingredients, and lays a solid foundation for the subsequent concentration and drying steps.

[0063] S5, concentration and drying, adding the mixture filtered and separated in S4 to a molecular distillation device 9, using molecular distillation technology to concentrate the liquid after centrifugation to remove the solvent, and using a vacuum freeze dryer 10 to dry the concentrated liquid to obtain a final extract powder;

[0064] Among them, when the liquid after centrifugation is concentrated by molecular distillation technology, the heating temperature is 45°C, the pressure is 10mbar, and the condensation temperature is 12°C;

[0065] When the concentrated liquid is dried using the vacuum freeze dryer 10, it is divided into three stages, wherein;

[0066] The pre-freeze drying temperature was -45 °C, the time was 5 h, and the pressure was 15 mbar;

[0067] The primary drying temperature is -10°C, the time is 20h, and the pressure is 15mbar;

[0068] The secondary drying temperature was 25°C, the time was 10 h, and the pressure was 15 mbar.

[0069] Specifically, in this step, separation is achieved based on the differences in the evaporation and condensation characteristics of molecules of different substances under high vacuum conditions. Under lower pressure and temperature conditions, solvents and some light components are easier to evaporate, while the target active ingredients remain in the residual liquid due to their higher boiling points or larger molecular weights. This can effectively remove the solvent and some volatile impurities in the extract, while reducing the thermal decomposition of heat-sensitive substances, which helps to maintain the integrity of the active ingredients. Three stages of low-temperature drying are adopted. The pre-freeze-drying stage ensures that the moisture in the sample is completely solidified, creating a uniform ice crystal layer for the subsequent drying stage, which helps to maintain the structure of the sample. The purpose of the primary drying stage is to directly sublimate the ice crystals under a vacuum environment, that is, to convert from a solid state to a gaseous state, thereby removing most of the moisture. The secondary drying stage is to remove the remaining unfrozen moisture, which may be adsorbed on the solid substance or present in the crystal structure;

[0070] In summary, this step utilizes molecular distillation and vacuum freeze-drying methods to concentrate and dry the extract, which effectively removes the solvent and water in the extract, obtains a high-purity extract powder rich in active ingredients, and retains the biological activity and stability of these ingredients to the greatest extent.

[0071] Embodiment 3,

[0072] The difference from Example 2 is that the process parameters in each step are specifically as follows:

[0073] In S1, the frequency of ultrasonic cleaning equipment 1 is 35KHz and the power is 0.7W / cm 2 , cleaning temperature is 40℃, cleaning time is 20min, drying temperature is 50℃, drying time is 5H, and drying is uniform;

[0074] In S2, the temperature of the freezing grinder 3 is -30°C, the grinding speed is 1200r / min, the grinding time is 12min, the frequency of the ultrasonic wall breaking machine 4 is 30KHz, and the power is 0.8W / cm 2 , time is 12min;

[0075] In S3, the stirring speed of the stirrer 5 is 120r / min, the time is 12min, the temperature is 20°C, the pressure of the supercritical CO2 extraction device is 180bar, the temperature is 40°C, the time is 25min, and the CO2 flow rate is 2L / h;

[0076] In S4, the pore size of the ceramic membrane filter 7 is 0.3 μm, the temperature is 24° C., the pressure is 12 bar, the speed of the high-speed centrifuge 8 is 10000 r / min, the time is 15 min, the temperature is 18° C., and the relative centrifugal force is 1000 g;

[0077] In S5, when the liquid after centrifugation is concentrated by molecular distillation technology, the heating temperature is 60°C, the pressure is 20 mbar, and the condensation temperature is 15°C;

[0078] When the concentrated liquid is dried using the vacuum freeze dryer 10, it is divided into three stages, wherein;

[0079] The pre-freeze drying temperature was -50 °C, the time was 5 h, and the pressure was 20 mbar;

[0080] The primary drying temperature is 0°C, the time is 30h, and the pressure is 20mbar;

[0081] The secondary drying temperature is 30°C, the time is 20 h, and the pressure is 20 mbar.

[0082] Embodiment 4,

[0083] The difference from Example 3 is that the process parameters in each step are specifically as follows:

[0084] In S1, the frequency of ultrasonic cleaning equipment 1 is 40KHz and the power is 0.8W / cm 2 , cleaning temperature 45℃, cleaning time 25min, drying temperature 55℃, drying time 6H, uniform drying;

[0085] In S2, the temperature of the freezing grinder 3 is -10°C, the grinding speed is 1400r / min, the grinding time is 15min, the frequency of the ultrasonic wall breaking machine 4 is 35KHz, and the power is 1W / cm 2 , time is 13min;

[0086] In S3, the stirring speed of the stirrer 5 is 160r / min, the time is 15min, the temperature is 50°C, the pressure of the supercritical CO2 extraction device is 200bar, the temperature is 40°C, the time is 30min, and the CO2 flow rate is 4L / h;

[0087] In S4, the pore size of the ceramic membrane filter 7 is 0.5 μm, the temperature is 24°C, the pressure is 15 bar, the speed of the high-speed centrifuge 8 is 11000 r / min, the time is 18 min, the temperature is 20°C, and the relative centrifugal force is 1200 g;

[0088] In S5, when the liquid after centrifugation is concentrated by molecular distillation technology, the heating temperature is 70°C, the pressure is 25 mbar, and the condensation temperature is 20°C;

[0089] When the concentrated liquid is dried using the vacuum freeze dryer 10, it is divided into three stages, wherein;

[0090] The pre-freeze drying temperature was -55 °C, the time was 5 h, and the pressure was 25 mbar;

[0091] The primary drying temperature is 10°C, the time is 40h, and the pressure is 25mbar;

[0092] The secondary drying temperature is 35°C, the time is 25h, and the pressure is 25mbar.

[0093] The following tests were conducted on various indices of the turmeric-wall-broken Ganoderma lucidum spore powder extraction process of Examples 2, 3 and 4 of the present invention, with the traditional extraction method used as a comparative example. The test results are shown in the following table:

[0094] Among them, the extraction rate of active ingredients, comparing the content of active ingredients in raw materials and final extracts, detection methods: high performance liquid chromatography or mass spectrometry;

[0095] Purity and impurity removal effect, evaluate the efficiency of physical and chemical means to remove impurities, detection method: atomic absorption spectroscopy or gas chromatography-mass spectrometry;

[0096] Bioactivity retention effect, evaluate the antioxidant, anti-inflammatory and other biological activities of the extracts, detection methods: enzyme-linked immunosorbent assay or cell viability test;

[0097] Product stability, test the stability of the extract under different conditions, test method: accelerated stability test chamber or UV-visible spectroscopy.

[0098]

[0099] According to the data in the above table, the comparative documents 2-4 in the present invention have better effects than the comparative examples, which are manifested in that the active ingredient extraction rate, purity and impurity removal rate reach more than 90%, the biological activity retention rate reaches more than 85%, and the product stability rate reaches more than 95%;

[0100] It can be seen that the present invention utilizes cryogenic grinding and ultrasonic wall breaking technology to destroy the raw material cell structure, and carries out mixed extraction with enzymes and ethanol in a specific ratio, as well as optimized stirring conditions, to ensure the full release of active ingredients and the maximization of extraction efficiency. Low temperature control, such as cryogenic grinding, molecular distillation and vacuum freeze drying, is adopted in the extraction and concentration process, which effectively avoids the loss of heat-sensitive substances and the reduction of biological activity that may be caused by high temperature. Supercritical CO2 extraction technology and molecular distillation are adopted to reduce the use of organic solvents, meet environmental protection requirements, and improve operational safety. Impurities and non-target components are removed through precisely controlled ceramic membrane filtration and high-speed centrifugal separation steps, thereby ensuring the high purity and quality consistency of the final product.

[0101] In conclusion, the present invention has achieved a series of sophisticated process flows for the efficient extraction and purification of active ingredients in turmeric root and ganoderma lucidum spore powder.

[0102] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A process for extracting turmeric wall-broken Ganoderma lucidum spore powder, characterized in that: The process comprises an ultrasonic cleaning device (1), a drying device (2) is arranged on one side of the ultrasonic cleaning device (1), a freezing grinder (3) is arranged on one side of the freezing grinder (3), an ultrasonic wall-breaking machine (4) is arranged on one side of the ultrasonic wall-breaking machine (4), a stirrer (5) is arranged on one side of the stirrer (5), a supercritical CO2 extraction device (6) is arranged on one side of the supercritical CO2 extraction device (6), a ceramic membrane filter (7) is arranged on one side of the ceramic membrane filter (7), a high-speed centrifuge (8) is arranged on one side of the high-speed centrifuge (8), a molecular distillation device (9) is arranged on one side of the high-speed centrifuge (8), and a vacuum freeze dryer (10) is arranged on one side of the molecular distillation device (9); The process adopts the above equipment for preparation, and specifically comprises the following steps: S1, raw material processing, fresh turmeric root and ganoderma lucidum spore powder are cleaned by ultrasonic cleaning equipment (1) to remove impurities inside, and then taken out and dried by drying equipment (2); S2, wall breaking treatment, using a freezing grinder (3) and an ultrasonic wall breaking machine (4) to break the wall of the dried turmeric root and ganoderma lucidum spore powder respectively, so as to release the active ingredients in the turmeric root and ganoderma lucidum spore powder; S3, mixed extraction, adding the broken turmeric root and ganoderma lucidum spore powder into a stirrer (5), adding 10% of the total content of turmeric root and ganoderma lucidum spore powder, enzyme and ethanol, as well as polysorbate surfactant and polyamide dispersant to mix, to obtain a mixture, adding the mixture to a supercritical CO2 extraction device, and extracting the mixture using supercritical CO2 extraction technology to dissolve the active ingredients; S4, filtering and separating, adding the mixture extracted in S3 to a ceramic membrane filter (7) for preliminary filtration to remove solid residues, and centrifuging the filtered mixture using a high-speed centrifuge (8) to separate the active ingredients, and adding phosphate buffer for washing to remove non-target ingredients; S5, concentration and drying, adding the mixture filtered and separated in S4 to a molecular distillation device (9), using molecular distillation technology to concentrate the liquid after centrifugation to remove the solvent, and using a vacuum freeze dryer (10) to dry the concentrated liquid to obtain a final extract powder; In S1, when the turmeric root and the ganoderma lucidum spore powder are cleaned by the ultrasonic cleaning device (1), the frequency of the ultrasonic cleaning device (1) is 25-40KHz and the power is 0.3-0.8W / cm 2 , cleaning temperature 30-50℃, cleaning time 10-30min; When the cleaned turmeric root and ganoderma lucidum spore powder are dried by the drying device (2), the drying temperature is 40-60° C., the drying time is 2-6 hours, and the drying is carried out evenly; In S2, when the dried turmeric root and ganoderma lucidum spore powder are subjected to wall breaking treatment by using a freezing grinder (3) and an ultrasonic wall breaking machine (4), the temperature of the freezing grinder (3) is -50°C to 0°C, the grinding speed is 800-1600 r / min, and the grinding time is 8-15 min; Ultrasonic wall breaking machine (4) with a frequency of 20-40KHz and a power of 0.3-1.0W / cm 2 , time is 8-15min; In S3, the broken turmeric root and ganoderma lucidum spore powder are added to the stirrer (5), and when the enzyme and ethanol with a total content of 10% of the turmeric root and ganoderma lucidum spore powder and the polysorbate surfactant and the polyamide dispersant are added and mixed, the stirrer (5) is stirred at a speed of 80-180 r / min, the time is 6-17 min, and the temperature is 45-50° C.; In S3, the mixture is added to a supercritical CO2 extraction device. When the mixture is extracted using supercritical CO2 extraction technology, the pressure of the supercritical CO2 extraction device is 100-300 bar, the temperature is 35-40° C., the time is 15-35 min, and the CO2 flow rate is 0-5 L / h; In S4, when the mixture extracted in S3 is added to the ceramic membrane filter (7) for preliminary filtration, the pore size of the ceramic membrane filter (7) is 0.1-0.5 μm, the temperature is 20-25° C., and the pressure is 5-20 bar; The filtered mixture is centrifuged using a high-speed centrifuge (8) to separate the active ingredient. The speed of the high-speed centrifuge (8) is 5000-12000 r / min, the time is 5-20 min, the temperature is 4-22° C., and the relative centrifugal force is 500-1500 g; In S5, the mixture separated by filtration in S4 is added to a molecular distillation device (9), and the liquid after centrifugation is concentrated by molecular distillation technology, with a heating temperature of 40-80°C, a pressure of 1-50 mbar, and a condensation temperature of 10-20°C; When the concentrated liquid is dried using a vacuum freeze dryer (10), it is divided into three stages, wherein: The pre-freeze drying temperature is -40 to -60°C, the time is 4-6 hours, and the pressure is 10-30 mbar; The primary drying temperature is -20 to 20°C, the time is 18-48h, and the pressure is 10-30mbar; The secondary drying temperature is 20-40°C, the time is 5-28h, and the pressure is 10-30mbar.

2. The process for extracting turmeric and broken spore powder of Ganoderma lucidum according to claim 1, characterized in that: The above-mentioned device is also connected to a control system (11) and a monitoring system (12) for controlling each device, wherein the monitoring system (12) uses sensors to monitor the key parameters of each step in real time, including temperature, pressure and flow rate, and the control system (11) controls the device to adjust the parameters according to the data from the sensors.

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