A topical composition for promoting hair growth and its preparation method and application

Abstract

The present invention relates to the technical field of hair growth agents, and in particular to an external composition for promoting hair growth, a preparation method and an application thereof. The raw materials of the external composition for promoting hair growth include: croaker scale polypeptide, croaker scale peptide magnesium chelate, carboxymethyl chitosan, β-sodium glycerophosphate, hyaluronic acid, β-cyclodextrin, menthol and sweet orange essential oil. The preparation method of the croaker scale peptide magnesium chelate includes: enzymatically hydrolyzing golden croaker scales, and then freeze-drying to obtain the croaker scale polypeptide; dissolving the croaker scale polypeptide in water, and then adding magnesium salt for chelation reaction to obtain the croaker scale peptide magnesium chelate. The structure of the croaker scale peptide magnesium chelate makes magnesium more easily absorbed by the human body, thereby improving the overall effect of the composition for promoting hair growth. The external composition for promoting hair growth of the present invention has the advantages of being safer, more convenient to use and having a wider range of applications than commercially available hair growth agents.

Description

Technical Field

[0001] The present invention relates to the technical field of hair growth agents, and in particular to an external composition for promoting hair growth, a preparation method and an application thereof. Background Art

[0002] The development of biological drugs is a hot topic in the current medical field, but this type of drug is not perfect and there are a series of problems and challenges.

[0003] Among the current hair growth drugs, Western medicines have safety risks, while Chinese medicines have problems such as long onset of effect and limited use.

[0004] Therefore, providing a hair growth promoting external composition with high safety and short onset time is of great significance in the technical field of hair growth agents. Summary of the invention

[0005] Based on the above content, the present invention provides an external composition for promoting hair growth, and a preparation method and application thereof.

[0006] To achieve the above object, the present invention provides the following solutions:

[0007] One of the technical solutions of the present invention is a method for preparing a magnesium chelate of croaker scale peptide, comprising the following steps:

[0008] The golden croaker scale is enzymatically hydrolyzed and then freeze-dried to obtain the golden croaker scale polypeptide;

[0009] The croaker scale polypeptide is dissolved in water, and then magnesium salt is added to carry out a chelation reaction to obtain the croaker scale peptide magnesium chelate.

[0010] Furthermore, papain is used for the enzymolysis; the conditions for the enzymolysis are: enzyme amount 1000-2000 U / g, solid-liquid ratio 1:(10-15) g / mL, temperature 50-70°C, pH 4.0-8.0, and time 4-8h.

[0011] Furthermore, the temperature of the chelating reaction is 60-75°C, and the time is 20-40 min; the mass ratio of the croaker scale polypeptide to the magnesium salt is (1.5-2):1; and the magnesium salt is MgCl2.

[0012] Before adding the magnesium salt, the method further comprises adjusting the pH value of the solution to be neutral using an acid or a base.

[0013] After the chelating reaction is completed, the steps of washing and purification are also included.

[0014] The second technical solution of the present invention is a magnesium chelate of croaker scale peptide prepared according to the above-mentioned preparation method.

[0015] The third technical solution of the present invention is the use of the above-mentioned croaker scale peptide magnesium chelate in the preparation of a hair growth agent.

[0016] The fourth technical solution of the present invention is a topical composition for promoting hair growth, which comprises, by weight, 1-10 parts of croaker scale polypeptide, 1-6 parts of the above-mentioned croaker scale peptide magnesium chelate, 0.1-2 parts of carboxymethyl chitosan, 0.5-5 parts of sodium β-glycerophosphate, 0.1-3 parts of hyaluronic acid, 0.1-2 parts of β-cyclodextrin, 0-2 parts of menthol and 0-1 parts of sweet orange essential oil.

[0017] A fifth technical solution of the present invention is a method for preparing the above-mentioned external composition for promoting hair growth, comprising the following steps:

[0018] Dissolve the croaker scale polypeptide, croaker scale peptide magnesium chelate and sodium β-glycerophosphate in water according to their weight proportions to obtain a S1 solution;

[0019] Dissolve hyaluronic acid in water, swell it, and mix it to obtain S2 solution;

[0020] Dissolving menthol in anhydrous ethanol to obtain S3 solution;

[0021] The S1 solution, the S2 solution and the S3 solution are uniformly mixed, and then β-cyclodextrin and sweet orange essential oil are added and mixed to obtain a S4 solution;

[0022] Add carboxymethyl chitosan into water and dissolve it to obtain S5 solution;

[0023] The S5 solution is added to the S4 solution to form a gel, thereby obtaining the external composition for promoting hair growth.

[0024] Furthermore, the gelation temperature is 30-45°C.

[0025] The sixth technical solution of the present invention is a hair growth agent, the raw materials of which include the above-mentioned croaker scale peptide magnesium chelate, or the above-mentioned external composition for promoting hair growth.

[0026] The present invention discloses the following technical effects:

[0027] Magnesium is a mineral essential for hair growth. Magnesium can also provide sufficient nutrition to the scalp and hair follicles by promoting blood circulation, and increase the vitality of hair follicle cells by participating in cell energy metabolism, thereby promoting hair growth. The croaker scale peptide has antioxidant properties and can help protect the scalp and hair follicles from free radical damage, which may cause hair to become brittle and fall out. The structure of the croaker scale peptide magnesium chelate of the present invention makes magnesium more easily absorbed by the human body, thereby improving the overall effect of the hair growth promoting composition.

[0028] Compared with commercially available hair growth agents, the external composition for promoting hair growth of the present invention has the advantages of being safer, more convenient to use and having a wider application range. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0030] Figure 1 The hair lengths of mice in each group at the second, third and fourth weeks after application of Application Example 1 of the present invention are shown.

[0031] Figure 2 These are macroscopic photographs of the hair growth of mice in each group after application and administration in Application Example 1 of the present invention.

[0032] Figure 3 The hair growth scores of mice in each group 14 days after application example 1 of the present invention.

[0033] Figure 4 The figure shows the changes in body weight of mice in each group after application example 1 of the present invention.

[0034] Figure 5 The organ indexes of mice in each group 7 days after application example 1 of the present invention were shown.

[0035] Figure 6 The expression of inflammatory factors (IL-1β, IL-12, TNF-α) in the skin of each group of mice was detected by ELISA method 7 days after the application example 1 of the present invention; wherein A is IL-1β, B is IL-12, and C is TNF-α.

[0036] Figure 7 HE staining was used to detect the changes in skin hair follicles and skin pathological conditions of each group of mice 7 days after the application example 1 of the present invention.

[0037] Figure 8 This is a photo of the hair growth effect after applying the hair growth composition prepared in Example 2 in Application Example 2 of the present invention for one month. DETAILED DESCRIPTION

[0038] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as limiting the present invention, but should be understood as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0039] It should be understood that the terms described in the present invention are only for describing a particular embodiment and are not intended to limit the present invention. In addition, for the numerical range in the present invention, it should be understood that each intermediate value between the upper and lower limits of the scope is also specifically disclosed. The intermediate value in any stated value or stated range, and each smaller range between any other stated value or intermediate value in the described range is also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded in the scope.

[0040] Unless otherwise indicated, all technical and scientific terms used herein have the same meanings as those generally understood by those skilled in the art. Although the present invention describes only preferred methods and materials, any methods and materials similar or equivalent to those described herein may also be used in the implementation or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials associated with the documents. In the event of a conflict with any incorporated document, the content of this specification shall prevail.

[0041] It will be apparent to those skilled in the art that various modifications and variations may be made to the specific embodiments of the present invention description without departing from the scope or spirit of the present invention. Other embodiments derived from the present invention description will be apparent to those skilled in the art. The present invention description and examples are exemplary only.

[0042] The words “include,” “including,” “have,” “contain,” etc. used in this document are open-ended terms, meaning including but not limited to.

[0043] The first aspect of the present invention provides a method for preparing a magnesium chelate of fish scale peptide, comprising the following steps:

[0044] Papain is used to enzymolyze golden croaker scales, and the enzymolysis conditions are as follows: enzyme amount 1000-2000 U / g, solid-liquid ratio 1:15-1:10 (g / mL), temperature 50-70°C, pH 4.0-8.0, time 4-8h, and the supernatant is passed through an ultrafiltration membrane (molecular weight 5KDa) to obtain enzymolyzates with molecular weight greater than 5KDa and molecular weight less than 5KDa, respectively, and the enzymolyzate with molecular weight less than 5KDa is freeze-dried to obtain croaker scale polypeptide (molecular weight less than 5KDa);

[0045] Take 0.1-3g of croaker scale polypeptide (molecular weight below 5000Da) and place it in a beaker, add 200mL of 45-75℃ ultrapure water, stir and dissolve, and adjust the pH value to 7.0 (adjust with 0.1mol / L HCl or 1.0mol / L NaOH);

[0046] Place the beaker in a 60-75℃ water bath, add 0.5g analytically pure MgCl2 (mass ratio of croaker scale polypeptide to MgCl2 2:1-3:2) under stirring, and allow the chelation reaction to proceed for 20-40min. After the reaction is complete, return to room temperature.

[0047] Add 100 mL of anhydrous ethanol to the above reaction solution, stir for 10 min, centrifuge (5000 r / min) for 10 min, transfer the precipitate to a 50 mL centrifuge tube, add 50 mL of anhydrous ethanol, shake and wash for 2 min, centrifuge (5000 r / min) for 10 min, separate the supernatant and detect free Mg 2+ , add 1.0 mol / L NaOH, if a white precipitate is produced, repeat the above steps until no Mg is detected 2+ until;

[0048] The obtained white precipitate is vacuum dried for 6-8 hours to obtain white crystals, which are the croaker scale peptide magnesium chelate, and are set aside.

[0049] The croaker scale polypeptide is naturally derived from the golden croaker scale. It is a renewable biological resource with good biodegradability and environmental protection. The collagen in the croaker scale polypeptide helps to increase the elasticity of the skin, promote skin metabolism, thereby improving the skin condition, promoting skin moisturizing, and maintaining the stability of the skin barrier.

[0050] Magnesium (Mg 2+ ) plays a vital role in more than 80% of all metabolic functions. Magnesium deficiency (hypomagnesemia) may play an important role in chronic diseases. The advantage of the ichthys scale peptide magnesium chelate of the present invention lies in the chelation of magnesium with peptides. This chelation has higher bioavailability and stability than traditional magnesium supplements, which helps the body to better absorb and utilize magnesium. The ichthys scale peptide magnesium chelate of the present invention can also relieve fatigue and maintain normal function of the nervous system, help maintain bone density, prevent osteoporosis, help maintain normal heart rhythm, reduce the risk of cardiovascular disease, and reduce muscle spasms and tension. In skin care products and nutritional supplements, ichthys scale peptide magnesium chelate may be used as an additive to enhance skin health, improve nutrient absorption and improve overall physical condition.

[0051] The second aspect of the present invention provides a magnesium chelate of croaker scale peptide prepared according to the above preparation method.

[0052] The third aspect of the present invention provides the use of the above-mentioned mullet scale peptide magnesium chelate in the preparation of a hair growth agent.

[0053] The fourth aspect of the present invention provides a topical composition for promoting hair growth, which comprises, by weight, 1-10 parts of croaker scale polypeptide, 1-6 parts of the above-mentioned croaker scale peptide magnesium chelate, 0.1-2 parts of carboxymethyl chitosan, 0.5-5 parts of sodium β-glycerophosphate, 0.1-3 parts of hyaluronic acid, 0.1-2 parts of β-cyclodextrin, 0-2 parts of menthol and 0-1 parts of sweet orange essential oil.

[0054] In the present invention, the mullet scale peptide magnesium chelate has the effect of promoting hair growth, and the mullet scale polypeptide can cooperate with the mullet scale peptide magnesium chelate to improve the hair growth effect; carboxymethyl chitosan has the effect of antibacterial and antiseptic; sodium β-glycerophosphate can cross-link carboxymethyl chitosan to prepare micro-nano particles, which plays the role of sustained release of active substances; hyaluronic acid plays the role of moisturizing and protecting the skin; menthol plays the role of stimulating blood circulation and eliminating fatigue; and sweet orange essential oil plays the role of refreshing.

[0055] The hair growth promoting external composition has obvious hair growth effect. The carboxymethyl chitosan is prepared into a sustained-release gel-loaded mullet scale polypeptide and mullet scale peptide magnesium chelate to achieve a sustained-release transdermal effect, improve the penetration effect of active ingredients, reduce skin oil, increase the transdermal amount of active ingredients, have a good transdermal effect, and achieve the purpose of promoting hair growth; the user acceptance and satisfaction are high.

[0056] A fifth aspect of the present invention provides a method for preparing the above-mentioned external composition for promoting hair growth, comprising the following steps:

[0057] S1: Dissolve the croaker scale polypeptide, croaker scale peptide magnesium chelate and sodium β-glycerophosphate in ultrapure water according to the above mass fractions (the mass volume ratio of croaker scale polypeptide to ultrapure water is (1-10) g:40 mL), stir and mix to obtain S1 solution;

[0058] S2: Dissolve hyaluronic acid in ultrapure water according to the above mass fractions (the mass volume ratio of hyaluronic acid to ultrapure water is (0.1-3) g:40 mL), and stir and mix thoroughly after swelling to obtain S2 solution;

[0059] S3: Dissolve menthol in anhydrous ethanol according to the above mass fractions (the mass volume ratio of menthol to anhydrous ethanol is (0.1-2) g:1 mL) to obtain S3 solution for later use;

[0060] S4: The above-mentioned S1, S2 and S3 solutions are mixed evenly, and the above-mentioned mass fractions of β-cyclodextrin and sweet orange essential oil are added, and the mixture is stirred thoroughly to obtain S4 solution;

[0061] S5: According to the above-mentioned mass fractions, carboxymethyl chitosan is added into ultrapure water (the mass volume ratio of carboxymethyl chitosan to ultrapure water is (0.1-2) g: 20 mL), and dissolved evenly to obtain S5 solution; the S5 solution is added dropwise into the S4 solution, stirred evenly, and placed in a constant temperature water bath at 30-45°C for gelation, thereby obtaining a carboxymethyl chitosan-based sustained-release hydrogel hair growth composition (a topical composition for promoting hair growth).

[0062] The sixth aspect of the present invention provides a hair growth agent, the raw materials of which include the above-mentioned ichthys scale peptide magnesium chelate, or the above-mentioned external composition for promoting hair growth.

[0063] Unless otherwise specified, the raw materials used in the embodiments of the present invention can be obtained through commercial sources.

[0064] The croaker scale polypeptide used in the embodiments of the present invention is prepared by the following steps:

[0065] Papain was used to enzymolyze the golden croaker scales. The enzymolysis conditions were as follows. The optimal preparation conditions were: enzyme amount 1500U / g, solid-liquid ratio 1:10 (g / mL), temperature 60℃, pH 6.0, and time 6h. After the enzymolysis was completed, the enzyme was inactivated in boiling water for 20min, cooled to room temperature and centrifuged at 12000r / min for 15min, and the supernatant was passed through an ultrafiltration membrane (molecular weight 5KDa) to obtain enzymolyzates with molecular weights greater than 5KDa and less than 5KDa, respectively. The enzymolyzate with a molecular weight less than 5KDa was freeze-dried to obtain croaker scale polypeptides (molecular weight less than 5KDa).

[0066] The croaker scale peptide magnesium chelate used in the embodiment of the present invention is prepared by the following steps:

[0067] S1, dissolution: Take 1g of croaker scale polypeptide (molecular weight below 5000Da) and place it in a beaker, add 200mL of 55℃ ultrapure water, stir and dissolve, and adjust the pH value to 7.0 (adjust with 0.1mol / L HCl or 1.0mol / L NaOH).

[0068] S2, chelation reaction: place the beaker in a 65°C water bath, add 0.5 g of analytically pure MgCl2 (mass ratio of croaker scale polypeptide to MgCl2: 2:1) with stirring, and allow the chelation reaction to proceed for 30 min. After the reaction is complete, allow the mixture to cool to room temperature.

[0069] S3, washing: add 100 mL of anhydrous ethanol to the above reaction solution, stir for 10 min, centrifuge (5000 r / min) for 10 min, and transfer the precipitate to a 50 mL centrifuge tube.

[0070] S4, purification: Take the precipitate obtained in S3, add 50 mL of anhydrous ethanol, shake and wash for 2 minutes, centrifuge (5000 r / min) for 10 minutes, separate the supernatant and detect free Mg 2+ , add 1.0 mol / L NaOH, if a white precipitate is produced, repeat the above steps until no Mg is detected 2+ until.

[0071] S5, drying: vacuum dry the precipitate obtained in S4 for 7 hours to obtain white crystals, which are the magnesium chelate of croaker scale peptide, and set aside.

[0072] The present invention is further described below by way of examples.

[0073] Example 1

[0074] A topical composition for promoting hair growth is prepared by the following steps:

[0075] Step 1, weigh 1 g of croaker scale polypeptide, 3 g of croaker scale peptide magnesium chelate and 0.5 g of sodium β-glycerophosphate, dissolve in 40 ml of ultrapure water, and stir to obtain S1 solution;

[0076] Step 2, weigh 200 mg of hyaluronic acid and dissolve it in 40 mL of ultrapure water, and stir and mix thoroughly after swelling to obtain S2 solution;

[0077] Step 3, weigh 1 mg of menthol and dissolve it in 1 mL of anhydrous ethanol to obtain S3 solution;

[0078] Step 4, mix the S1, S2 and S3 solutions evenly, add 500 mg of β-cyclodextrin and 10 μL of sweet orange essential oil, and stir well to obtain S4 solution;

[0079] Step 5, dissolving 0.5 g of carboxymethyl chitosan in 20 mL of ultrapure water and dissolving evenly to obtain S5 solution; adding the above S5 solution dropwise to the S4 solution, stirring evenly, and placing in a constant temperature water bath at 40° C. (30-45° C.) for gelation, thereby obtaining a carboxymethyl chitosan-based sustained-release hydrogel hair growth composition, which is packaged and sterilized under ultraviolet light to obtain a topical composition for promoting hair growth, referred to as the hair growth composition.

[0080] Example 2

[0081] The only difference from Example 1 is that the added amount of croaker scale peptide magnesium chelate is 6 g, and the other steps and parameters are the same as those in Example 1.

[0082] Comparative Example 1

[0083] The only difference from Example 1 is that the addition of croaker scale peptide magnesium chelate is omitted, and the remaining steps and parameters are the same as those in Example 1.

[0084] Application Example 1

[0085] The effects of the hair growth compositions prepared in Example 1, Example 2 and Comparative Example 1 as hair growth agents were verified as follows:

[0086] 1. Establishment of animal model of hair loss

[0087] 60 C57BL / 6 male mice were randomly divided into 6 groups, 10 mice in each group: blank group, model group, positive group, solvent group, 3% group, and 6% group. The mouse alopecia areata model was established by 6% Na2S hair removal method. Dip the prepared 6% Na2S with a cotton ball and apply it on the back of the mouse. The hair removal area is about 4cm 2 (2cm×2cm), hair removal was continued for 30 days, and skin color and hair length were observed every day.

[0088] Blank group: no hair removal, no medication, and normal feeding every day.

[0089] Model group: 2×2 cm area of ​​back hair removal, the hair removal area was smeared with normal saline (400 μl) once a day.

[0090] Positive group: 2×2 cm area of ​​back was depilated, and minoxidil tincture (two sprays) was applied to the depilated area once a day.

[0091] Solvent group: 2×2 cm area of ​​back was depilated, and the depilated area was smeared with the hair growth composition (400 μl) prepared in Comparative Example 1, once a day.

[0092] 3% group (3% peptide magnesium hair growth solution group): 2×2 cm area of ​​back hair removal, the hair removal area was smeared with the hair growth composition (400 μl) prepared in Example 1, once a day.

[0093] 6% group (6% peptide magnesium hair growth liquid group): 2×2 cm area of ​​back hair removal, the hair removal area was smeared with the hair growth composition (400 μl) prepared in Example 2, once a day.

[0094] Before each application and administration in each group, the back of the mice was scrubbed with normal saline and wiped dry with a cotton swab; the administration was repeated for 28 days.

[0095] 2. Detection of indicators

[0096] After the hair removal is completed, the mice are raised and the following indicators are observed and recorded:

[0097] Weight changes of mice: The mice were weighed every three days, and the weight changes and differences in mouse status of each group of mice were recorded.

[0098] Comparison of hair growth rate in mice: The hair growth length and growth area of ​​mice were observed regularly, and statistical analysis was performed on the hair growth of mice on the 6th, 9th, 12th and 15th days.

[0099] 3. Results Analysis

[0100] In this application example, the hair length of each group of mice in the second, third and fourth weeks after the application is as follows Figure 1 shown.

[0101] The macroscopic photos of the hair growth of each group of mice after application and administration in this application example are as follows: Figure 2 shown.

[0102] In this application example, the hair growth scores of each group of mice 14 days after application are as follows: Figure 3 shown.

[0103] In this application example, the weight changes of mice in each group after smearing and administration are as follows Figure 4 shown.

[0104] In this application example, the organ indexes of each group of mice 7 days after application are as follows: Figure 5 shown.

[0105] In this application example, 7 days after the application, the ELISA method was used to detect the expression of inflammatory factors (IL-1β, IL-12, TNF-α) in the skin of each group of mice. Figure 6 shown.

[0106] In this application example, HE staining was used to detect the changes in the skin hair follicles and skin pathology of each group of mice 7 days after the application. Figure 7 shown.

[0107] Depend on Figures 1 to 5 It can be seen that after hair removal and drug administration, there was no significant difference in the body weight of mice in each group, indicating that the hair removal agent and the hair growth agent had no significant effect on the body weight of mice (see Figure 4 ), there was no significant difference in organ indexes between mice (see Figure 5 ), the mice were in good condition, in good spirits, slightly increased in weight, and had normal food and water intake, indicating that the hair growth composition prepared by the present invention has good safety. The hair lengths of the positive group, solvent group, 3% group and 6% group were better than those of the model group, and the 3% group and 6% group were close to those of the positive drug group, indicating that the hair growth compositions prepared by Examples 1 and 2 of the present invention have good hair growth effects.

[0108] After 7 days of administration, the skin tissues of mice in each group were taken, and the expression levels of inflammatory factors IL-1β, IL-12 and TNF-α in the skin tissues of mice in each group were detected by ELISA. Figure 6As shown, the expression levels of inflammatory factors in the 3% group and the 6% group were lower than that in the model group. It can be seen that the hair growth composition has the effect of inhibiting the expression of inflammatory factors in skin tissue.

[0109] Seven days after administration, the skin tissues of mice in each group were fixed with paraformaldehyde, dehydrated, transparentized, stained, dehydrated and mounted, observed under a microscope, and images were collected and analyzed. The results are shown in Figure 7 As shown, in the model group, inflammatory cells aggregated, hair follicles were sparsely distributed, cavitation of hair follicle cells occurred, and there was bleeding around them. In the hair growth composition group, there was no inflammatory cell infiltration, and the hair follicles were clear and complete, indicating that the hair growth composition can promote the growth of hair follicles in mice.

[0110] Application Example 2

[0111] The hair growth composition prepared in Example 2 is quantitatively (1 cm) added to the hair growth composition at a fixed time every day (it can be morning or evening, depending on personal preference). 2 Apply about 100μL) to the fixed area with less hair around the top of the head, and take pictures every week to observe the specific hair growth. The photo of hair growth effect after one month of application is as follows Figure 8 As shown. Figure 8 It can be seen that the hair growth composition prepared in Example 2 of the present invention has a good hair growth effect.

[0112] On the basis of Example 2, the present invention also tried to replace the croaker scale peptide magnesium chelate with croaker scale polypeptide and magnesium salt in equal proportions, omit the addition of croaker scale polypeptide, and replace the croaker scale peptide magnesium chelate with other peptide chelates (that is, replacing croaker scale polypeptide with cod peptide and tilapia polypeptide purchased on the market to prepare peptide magnesium chelate) while keeping the other steps and parameters unchanged. The results showed that the hair growth effects of the hair growth compositions prepared above were not as good as the hair growth composition prepared in Example 2.

[0113] The embodiments described above are only descriptions of the preferred modes of the present invention, and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by ordinary technicians in this field should all fall within the protection scope determined by the claims of the present invention.

Claims

1. An application of a chelate of ichthys scale peptide magnesium in the preparation of a hair growth agent, characterized in that: The preparation method of the ichthys scale peptide magnesium chelate comprises the following steps: The golden croaker scale is enzymatically hydrolyzed and then freeze-dried to obtain the golden croaker scale polypeptide; Dissolving the croaker scale polypeptide in water, and then adding magnesium salt to carry out a chelation reaction to obtain the croaker scale peptide magnesium chelate; The enzymatic hydrolysis uses papain; the conditions of the enzymatic hydrolysis are: enzyme amount 1000-2000 U / g, solid-liquid ratio 1: (10-15) g / mL, temperature 50-70°C, pH 4.0-8.0, time 4-8h; The temperature of the chelating reaction is 60-75° C., and the time is 20-40 min. The mass ratio of the croaker scale polypeptide to the magnesium salt is (1.5-2):

1. The magnesium salt is MgCl2.

2. A composition for external use for promoting hair growth, characterized in that: The external composition for promoting hair growth is composed of the croaker scale polypeptide according to claim 1, the croaker scale peptide magnesium chelate according to claim 1, carboxymethyl chitosan, sodium β-glycerophosphate, hyaluronic acid, β-cyclodextrin, menthol and sweet orange essential oil.

3. A method for preparing the external composition for promoting hair growth according to claim 2, characterized in that: The following steps are involved: Dissolve the croaker scale polypeptide, croaker scale peptide magnesium chelate and sodium β-glycerophosphate in water according to the mass ratio to obtain S1 solution; Dissolve hyaluronic acid in water, swell it, and mix it to obtain S2 solution; Dissolving menthol in anhydrous ethanol to obtain S3 solution; The S1 solution, the S2 solution and the S3 solution are uniformly mixed, and then β-cyclodextrin and sweet orange essential oil are added and mixed to obtain a S4 solution; Add carboxymethyl chitosan into water and dissolve it to obtain S5 solution; The S5 solution is added to the S4 solution to form a gel, thereby obtaining the external composition for promoting hair growth.

4. The method for preparing the external composition for promoting hair growth according to claim 3, characterized in that: The gelling temperature is 30-45°C.

5. A hair growth agent, characterized in that: The raw materials include the external composition for promoting hair growth according to claim 2.

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