A kind of production process of Huoxiang Zhengqi water and its product

Abstract

The present invention relates to the technical field of Chinese patent medicines, and in particular to a production process of Huoxiang Zhengqi water and its products. The present invention decocts Poria cocos, dried tangerine peel, and raw pinellia tuber, filters to obtain a filtrate and a filter residue, and concentrates the filtrate to obtain a concentrate; Atractylodes lancea, dried tangerine peel, Magnolia officinalis, Angelica dahurica and the aforementioned filter residue are simultaneously extracted with an ethanol aqueous solution, concentrated, and then an extractant composed of an aqueous phase and an oil phase is efficiently extracted, and the extraction solvent is concentrated to obtain a concentrate; and then different concentrates are prepared together with liquorice extract, patchouli oil, perilla leaf oil, polyglycerol-10 stearate, sucrose ester SE-15, water and ethanol to prepare Huoxiang Zhengqi water, wherein the ethanol content in the Huoxiang Zhengqi water is as low as 35-45% and still has excellent stability, and has excellent antidiarrheal effect.

Description

Technical Field

[0001] The invention relates to the technical field of Chinese patent medicines, and in particular to a production process of Huoxiang Zhengqi water and a product thereof. Background Art

[0002] Huoxiang Zhengqi Shui, a Chinese patent medicine, is a summer heat-removing agent with the effects of dispelling dampness, regulating qi and harmonizing the middle. It is used for colds caused by exogenous wind-cold, internal injury and dampness, or summer heat and dampness, with symptoms of headache, heaviness, chest tightness, abdominal distension and pain, vomiting and diarrhea; gastrointestinal colds with the above symptoms.

[0003] Existing Huoxiang Zhengqi water products often have the defect of unstable storage, and the antidiarrhea effect needs to be further improved. Therefore, providing a Huoxiang Zhengqi water with improved antidiarrhea effect and stable storage is one of the technical problems that need to be solved. Summary of the invention

[0004] The present invention solves the above technical problems, and the scheme of the present invention includes a production process of Huoxiang Zhengqi Water and its product.

[0005] The technical solution of the present invention is specifically as follows:

[0006] As a scheme, the present invention provides a production process of Huoxiang Zhengqi water, wherein the process uses an ethanol aqueous solution to soak atractylodes, dried tangerine peel, magnolia bark, and angelica dahurica, and heats and refluxes to obtain a reflux liquid and a medicinal residue I, and then the reflux liquid is allowed to stand for stratification to obtain an oil layer material and a water layer material; water is added to Poria cocos, dried tangerine peel, and raw pinellia tuber, as well as the medicinal residue I and the water layer material, and the decocted, filtered, and the filtrate is concentrated to obtain a concentrate 1; then the concentrate 1 is added with an extractant composed of an aqueous phase and an oil phase (obtained by compounding cyclohexane and ethyl acetate) for extraction, and the extracted aqueous phase is taken and concentrated to obtain a concentrate 2; finally, the oil layer material, the concentrate 2, licorice extract, patchouli oil, perilla leaf oil, polyglycerol-10 stearate, sucrose ester SE-15, water and ethanol are stirred, and microporous filtration is performed to obtain the Huoxiang Zhengqi water.

[0007] Preferably, the production process of Huoxiang Zhengqi Water comprises the following steps:

[0008] Step S1: prepare raw materials: 145-165 g of Atractylodes lancea, 140-170 g of dried orange peel, 150-170 g of Magnolia officinalis, 230-260 g of Angelica dahurica, 230-260 g of Poria cocos, 230-260 g of Rhizoma Cibotii, 150-180 g of Raw Pinellia, 17-21 g of Licorice extract, 1.4-1.7 mL of Patchouli oil, 0.7-1 mL of Perilla leaf oil, polyglycerol-10 stearate, sucrose ester SE-15, ethanol, and water;

[0009] Step S2: grind Poria cocos, dried tangerine peel, and raw Pinellia tuber to obtain powder with a particle size of 0.5-100 mm, mix Atractylodes lancea, dried orange peel, Magnolia officinalis, and Angelica dahurica according to the above mass parts to obtain mixed powder 1, add water to boil, filter, and obtain a filtrate and a filter residue; concentrate the filtrate to obtain a concentrate 1;

[0010] Step S3: Grind Atractylodes lancea, dried orange peel, Magnolia officinalis, and Angelica dahurica respectively to obtain powder with a particle size of 0.5-100 mm, mix Poria cocos, dried orange peel, and raw Pinellia ternata according to the above mass parts to obtain mixed powder 2, then add ethanol aqueous solution and the filter residue of step S2, stir under heating, filter, and concentrate the filtrate to obtain concentrate 2;

[0011] Step S4: adding the concentrate 2 to an extractant consisting of an aqueous phase and an oil phase for extraction, taking the extracted oil phase, and concentrating under reduced pressure to remove cyclohexane and ethyl acetate to obtain a concentrate 3; wherein the aqueous phase in the extractant is water, and the oil phase is composed of cyclohexane and ethyl acetate in a mass ratio of 1:2.5-4;

[0012] Step S5: combining the concentrate 1 of step S2, the concentrate 3 of step S4, licorice extract, patchouli oil, perilla leaf oil, polyglycerol-10 stearate, sucrose ester SE-15, water and ethanol to obtain a combined product, adjusting the total volume of the combined product to 1800-2500 mL, then stirring evenly, and microfiltration to obtain Huoxiang Zhengqi water;

[0013] Among them, the total mass of polyglycerol-10 stearate and sucrose ester SE-15 accounts for 0.01-0.1% in Huoxiang Zhengqi water; the volume content of ethanol in Huoxiang Zhengqi water is 35-45%.

[0014] Preferably, in step S2, the mass ratio of the mixed powder 1 to water is 1:8-50, 1:10-40 or 1:12-30.

[0015] Preferably, in step S2, the boiling temperature is 85-95°C, 87-94°C or 88-93°C.

[0016] Preferably, in step S2, the decoction time is 4-12 hours, 5-11 hours or 5-10 hours.

[0017] Preferably, in step S2, the decoction process is performed by adding water to the initial decoction volume every half an hour.

[0018] Preferably, in step S2, the concentrate 1 is 0.5-1.5 times, 0.55-1.2 times or 0.6-1 times the mass of the mixed powder 1.

[0019] Preferably, in step S3, the volume concentration of the ethanol aqueous solution is 40-70%, 45-65%, 45-62% or 50-62%.

[0020] Preferably, in step S3, the mass ratio of the mixed powder 2 to the ethanol aqueous solution is 1:8-50, 1:10-40 or 1:12-30.

[0021] Preferably, in step S3, the heating temperature is 50-65°C, 52-63°C or 55-60°C.

[0022] Preferably, in step S3, the stirring speed is 50-500 rpm, 55-400 rpm or 60-300 rpm.

[0023] Preferably, in step S3, the stirring time is 5-20 hours, 6-18 hours or 7-15 hours.

[0024] Preferably, in step S3 , the concentrate 2 is 0.5-1.5 times, 0.55-1.2 times or 0.6-1 times the mass of the mixed powder 2 .

[0025] Preferably, in step S4, the mass ratio of the concentrate 2 to the extractant is 1:5-50, 1:6-30 or 1:10-20.

[0026] Preferably, in step S4, the mass ratio of the water phase to the oil phase is 1:0.5-1.5, 1:0.6-1.3 or 1:0.7-1.

[0027] Preferably, in step S4, the mass ratio of cyclohexane to ethyl acetate in the oil phase may also be 1:3-4, 1:3.2-4 or 1:3.2-3.8.

[0028] Preferably, in step S4, the extraction temperature is 40-55°C, 42-55°C or 45-50°C.

[0029] Preferably, in step S4, the extraction time is 2-7 hours, 3-6.5 hours or 4-6 hours.

[0030] Preferably, in step S5, the total mass of polyglycerol-10 stearate and sucrose ester SE-15 in Huoxiang Zhengqi water may account for 0.02-0.09%, 0.04-0.08% or 0.05-0.075%.

[0031] Preferably, in step S5, the mass ratio of polyglycerol-10 stearate to sucrose ester SE-15 is 1:2-4, 1:2.5-3.5 or 1:2.6-3.2.

[0032] Preferably, in step S5, the stirring speed is 100-5000 rpm, 200-3000 rpm or 300-1000 rpm, and the stirring time is 2-5 hours, 2.5-4 hours or 2.5-3.5 hours.

[0033] Preferably, in step S5, the volume content of ethanol in Huoxiang Zhengqi water is 35-44%, 35-42% or 36-39%.

[0034] On the other hand, the present invention also provides a product Huoxiang Zhengqi Water obtained by the preparation method.

[0035] Beneficial effects:

[0036] The invention boils Poria cocos, dried orange peel and raw Pinellia tuber, obtains filtrate and filter residue by filtering, and concentrates the filtrate to obtain a concentrate; extracts Atractylodes lancea, dried orange peel, Magnolia officinalis, Angelica dahurica and the filter residue simultaneously with an ethanol aqueous solution, and concentrates, and then extracts efficiently with an extractant composed of an aqueous phase and an oil phase, and removes the extraction solvent by concentration to obtain a concentrate; and then prepares Huoxiang Zhengqi water by combining different concentrates with liquorice extract, patchouli oil, perilla leaf oil, polyglycerol-10 stearate, sucrose ester SE-15, water and ethanol, wherein the ethanol content in the Huoxiang Zhengqi water is as low as 35-45%, the water still has excellent stability, and the antidiarrheal effect is excellent.

[0037] When the Huoxiang Zhengqi water obtained by the present invention is tested for antidiarrhea in rats with senna diarrhea model, the diarrhea index value is shown to reach 0.79±0.09. The Huoxiang Zhengqi water obtained by the present invention can effectively inhibit diarrhea.

[0038] The present invention uses a composite extractant extraction process to more efficiently extract the active ingredients in the filter residues of Atractylodes lancea, dried orange peel, Magnolia officinalis, Angelica dahurica, Poria cocos, Cortex tangerineae, and raw Pinellia tuber, so that the types and contents of the effective ingredients in Huoxiang Zhengqi water are more abundant, and the antidiarrheal effect of Huoxiang Zhengqi water is increased. The combination of cyclohexane and ethyl acetate in the composite extractant of the present invention can produce a mutually reinforcing relationship, and the two promote synergistically to increase the antidiarrheal effect of Huoxiang Zhengqi water. And when the mass ratio of cyclohexane to ethyl acetate in the extractant is 1:2.5-4, Huoxiang Zhengqi water shows a better antidiarrheal effect.

[0039] The Huoxiang Zhengqi water obtained by the present invention is diluted with a dilution solvent and the conductivity is adjusted to 50S / cm, and the conductivity is -38.47mV. The Huoxiang Zhengqi water of the present invention has good stability. After being stored at a high temperature of 40°C for 60 days, the conductivity is -30.52mV, which still has good stability. The Huoxiang Zhengqi water prepared by the present invention still maintains a good stability effect after being affected by an adverse environment.

[0040] The addition of polyglycerol-10 stearate and sucrose ester SE-15 to the Huoxiang Zhengqi water of the present invention can give the product better stability. The combination of the two makes the Huoxiang Zhengqi water have a higher absolute value of Zeta potential after storage at room temperature and high temperature, giving the product excellent stability. In the preparation system of the present invention, especially when the mass ratio of polyglycerol-10 stearate and sucrose ester SE-15 is 1:2-4, the stability of Huoxiang Zhengqi water shows a more favorable change trend. In the preparation system of the present invention, after using other materials, such as Tween-60, to replace the combination of polyglycerol-10 stearate and sucrose ester SE-15, the stability of the obtained Huoxiang Zhengqi water shows an unfavorable decrease, which cannot meet the requirements of container storage, etc., so that its use effect is reduced.

[0041] The present invention uses a composite extraction process, which has a certain influence on the stability of the Huoxiang Zhengqi water finally prepared.

[0042] The Huoxiang Zhengqi water prepared by the invention has excellent performance and good application prospect. DETAILED DESCRIPTION

[0043] The present invention will be described below in conjunction with specific embodiments, and various effects of the present invention will be more clearly presented. It should be understood by those skilled in the art that these specific embodiments are used to illustrate the present invention, rather than to limit the present invention.

[0044] Unless otherwise specified, various raw materials, reagents, instruments and equipment used in the present invention can be purchased from the market or prepared by existing methods.

[0045] 1. Production of Huoxiang Zhengqi Water

[0046] Huoxiang Zhengqi Water 1: The production process is as follows:

[0047] Step S1: prepare raw materials: 150 g of Atractylodes lancea, 160 g of dried orange peel, 160 g of Magnolia officinalis, 240 g of Angelica dahurica, 245 g of Poria, 250 g of Rhizoma Citri Reticulatae, 170 g of Raw Pinellia, 19 g of Licorice extract, 1.5 mL of Patchouli oil, 0.8 mL of Perilla leaf oil, polyglycerol-10 stearate, sucrose ester SE-15, ethanol, and water;

[0048] Step S2: grind Poria cocos, dried orange peel, and raw Pinellia tuber respectively to obtain a powder with a particle size of 0.5-100 mm, mix Atractylodes lancea, dried orange peel, Magnolia officinalis, and Angelica dahurica according to the above mass parts to obtain a mixed powder 1, add water 20 times the mass of the mixed powder 1, and boil at a temperature of 90° C. for 6.5 hours. During the boiling process, water is added to the initial boiling volume every half an hour; filter the decoction with a filter membrane with a pore size of 10 microns to obtain a filtrate and a filter residue; and concentrate the filtrate under reduced pressure at 62° C. to 0.7 times the mass of the mixed powder 1 to obtain a concentrate 1;

[0049] Step S3: Grind Atractylodes lancea, dried orange peel, Magnolia officinalis, and Angelica dahurica respectively to obtain a powder with a particle size of 0.5-100 mm, mix Poria cocos, dried orange peel, and raw Pinellia ternata according to the above mass parts to obtain mixed powder 2, then add a 55% ethanol aqueous solution with a volume concentration of 15 times the mass of the mixed powder 2 and the filter residue of step S2, stir at 80 rpm for 10 hours under the condition of heating to 58° C., filter through a filter membrane with a pore size of 10 microns, and concentrate the filtrate at 60° C. under reduced pressure to 0.8 times the mass of the mixed powder 2 to obtain concentrate 2;

[0050] Step S4: adding the concentrate 2 to an extractant consisting of an aqueous phase and an oil phase, extracting at a temperature of 48° C. for 4 hours, taking the extracted oil phase, and concentrating under reduced pressure at a temperature of 53° C. and a pressure of 0.025 MPa to remove cyclohexane and ethyl acetate, and stopping the reduced pressure concentration when the concentrate does not contain cyclohexane and ethyl acetate, to obtain a concentrate 3; wherein the mass ratio of the concentrate 2 to the extractant is 1:10; in the extractant, the aqueous phase is water, the oil phase is composed of cyclohexane and ethyl acetate in a mass ratio of 1:3, and the mass ratio of the aqueous phase to the oil phase is 1:0.8;

[0051] Step S5: combining the concentrate 1 of step S2, the concentrate 3 of step S4, licorice extract, patchouli oil, perilla leaf oil, polyglycerol-10 stearate, sucrose ester SE-15, water and ethanol to obtain a combined product, adjusting the total volume of the combined product to 2200 mL, stirring the combined product at a speed of 400 rpm for 2.5 hours to ensure uniform stirring, and filtering the combined product through a microporous filter with a pore size of 0.5 μm to obtain Huoxiang Zhengqi Water;

[0052] Among them, the total mass of polyglycerol-10 stearate and sucrose ester SE-15 accounts for 0.08% in Huoxiang Zhengqi water, the mass ratio of polyglycerol-10 stearate and sucrose ester SE-15 is 1:3, and the volume content of ethanol in Huoxiang Zhengqi water is 38%.

[0053] Huoxiang Zhengqi Water 2: The production process is as follows:

[0054] Step S1: prepare raw materials: 145 g of Atractylodes lancea, 165 g of dried orange peel, 165 g of Magnolia officinalis, 240 g of Angelica dahurica, 240 g of Poria cocos, 245 g of dried tangerine peel, 175 g of raw Pinellia tuber, 18.8 g of Licorice extract, 1.5 mL of Patchouli oil, 0.8 mL of Perilla leaf oil, polyglycerol-10 stearate, sucrose ester SE-15, ethanol, and water;

[0055] Step S2: grind Poria cocos, dried orange peel, and raw Pinellia tuber respectively to obtain a powder with a particle size of 0.5-100 mm, mix Atractylodes lancea, dried orange peel, Magnolia officinalis, and Angelica dahurica according to the above mass parts to obtain a mixed powder 1, add water 22 times the mass of the mixed powder 1, and boil at a temperature of 88° C. for 7 hours. During the boiling process, water is added to the initial boiling volume every half an hour; filter the decoction with a filter membrane with a pore size of 10 microns to obtain a filtrate and a filter residue; and concentrate the filtrate under reduced pressure at 62° C. to 0.72 times the mass of the mixed powder 1 to obtain a concentrate 1;

[0056] Step S3: Grind Atractylodes lancea, dried orange peel, Magnolia officinalis, and Angelica dahurica respectively to obtain a powder with a particle size of 0.5-100 mm, mix Poria cocos, dried orange peel, and raw Pinellia ternata according to the above mass parts to obtain mixed powder 2, then add a 55% ethanol aqueous solution with a volume concentration of 14 times the mass of the mixed powder 2 and the filter residue of step S2, stir at 75 rpm for 10 hours under the condition of heating to 56° C., filter through a filter membrane with a pore size of 10 microns, and concentrate the filtrate at 60° C. under reduced pressure to 0.78 times the mass of the mixed powder 2 to obtain concentrate 2;

[0057] Step S4: adding the concentrate 2 to an extractant consisting of an aqueous phase and an oil phase, extracting at a temperature of 48° C. for 4.5 hours, taking the extracted oil phase, and concentrating under reduced pressure at a temperature of 53° C. and a pressure of 0.025 MPa to remove cyclohexane and ethyl acetate, and stopping the reduced pressure concentration when the concentrate does not contain cyclohexane and ethyl acetate, to obtain a concentrate 3; wherein the mass ratio of the concentrate 2 to the extractant is 1:15; in the extractant, the aqueous phase is water, the oil phase is composed of cyclohexane and ethyl acetate in a mass ratio of 1:3.5, and the mass ratio of the aqueous phase to the oil phase is 1:1;

[0058] Step S5: combining the concentrate 1 of step S2, the concentrate 3 of step S4, licorice extract, patchouli oil, perilla leaf oil, polyglycerol-10 stearate, sucrose ester SE-15, water and ethanol to obtain a combined product, adjusting the total volume of the combined product to 2180 mL, stirring the combined product at a speed of 400 rpm for 3 hours to ensure uniform stirring, and filtering the combined product through a 0.5 μm pore size microporous filter to obtain Huoxiang Zhengqi water;

[0059] Among them, the total mass of polyglycerol-10 stearate and sucrose ester SE-15 in Huoxiang Zhengqi water accounts for 0.082%, the mass ratio of polyglycerol-10 stearate and sucrose ester SE-15 is 1:4, and the volume content of ethanol in Huoxiang Zhengqi water is 37%.

[0060] Huoxiang Zhengqi Water 3: The production process is as follows:

[0061] Step S1: prepare raw materials: 150 g of Atractylodes lancea, 160 g of dried orange peel, 160 g of Magnolia officinalis, 240 g of Angelica dahurica, 245 g of Poria, 250 g of Rhizoma Citri Reticulatae, 170 g of Raw Pinellia, 19 g of Licorice extract, 1.5 mL of Patchouli oil, 0.8 mL of Perilla leaf oil, polyglycerol-10 stearate, sucrose ester SE-15, ethanol, and water;

[0062] Step S2: grind Poria cocos, dried orange peel, and raw Pinellia tuber respectively to obtain a powder with a particle size of 0.5-100 mm, mix Atractylodes lancea, dried orange peel, Magnolia officinalis, and Angelica dahurica according to the above mass parts to obtain a mixed powder 1, add water 20 times the mass of the mixed powder 1, and boil at a temperature of 90° C. for 6.5 hours. During the boiling process, water is added to the initial boiling volume every half an hour; filter the decoction with a filter membrane with a pore size of 10 microns to obtain a filtrate and a filter residue; and concentrate the filtrate under reduced pressure at 62° C. to 0.7 times the mass of the mixed powder 1 to obtain a concentrate 1;

[0063] Step S3: Grind Atractylodes lancea, dried orange peel, Magnolia officinalis, and Angelica dahurica respectively to obtain a powder with a particle size of 0.5-100 mm, mix Poria cocos, dried orange peel, and raw Pinellia ternata according to the above mass parts to obtain mixed powder 2, then add a 55% ethanol aqueous solution with a volume concentration of 15 times the mass of the mixed powder 2 and the filter residue of step S2, stir at 80 rpm for 10 hours under the condition of heating to 58° C., filter through a filter membrane with a pore size of 10 microns, and concentrate the filtrate at 60° C. under reduced pressure to 0.8 times the mass of the mixed powder 2 to obtain concentrate 2;

[0064] Step S4: adding the concentrate 2 to an extractant consisting of an aqueous phase and an oil phase, extracting at a temperature of 48° C. for 4 hours, taking the extracted oil phase, and concentrating under reduced pressure at a temperature of 53° C. and a pressure of 0.025 MPa to remove cyclohexane and ethyl acetate, and stopping the reduced pressure concentration when the concentrate does not contain cyclohexane to obtain a concentrate 3; wherein the mass ratio of the concentrate 2 to the extractant is 1:10; in the extractant, the aqueous phase is water, the oil phase is cyclohexane, and the mass ratio of the aqueous phase to the oil phase is 1:0.8;

[0065] Step S5: combining the concentrate 1 of step S2, the concentrate 3 of step S4, licorice extract, patchouli oil, perilla leaf oil, polyglycerol-10 stearate, sucrose ester SE-15, water and ethanol to obtain a combined product, adjusting the total volume of the combined product to 2200 mL, stirring the combined product at a speed of 400 rpm for 2.5 hours to ensure uniform stirring, and filtering the combined product through a microporous filter with a pore size of 0.5 μm to obtain Huoxiang Zhengqi Water;

[0066] Among them, the total mass of polyglycerol-10 stearate and sucrose ester SE-15 accounts for 0.08% in Huoxiang Zhengqi water, the mass ratio of polyglycerol-10 stearate and sucrose ester SE-15 is 1:3, and the volume content of ethanol in Huoxiang Zhengqi water is 38%.

[0067] Huoxiang Zhengqi Water 4: The production process is as follows:

[0068] Step S1: prepare raw materials: 150 g of Atractylodes lancea, 160 g of dried orange peel, 160 g of Magnolia officinalis, 240 g of Angelica dahurica, 245 g of Poria, 250 g of Rhizoma Citri Reticulatae, 170 g of Raw Pinellia, 19 g of Licorice extract, 1.5 mL of Patchouli oil, 0.8 mL of Perilla leaf oil, polyglycerol-10 stearate, sucrose ester SE-15, ethanol, and water;

[0069] Step S2: grind Poria cocos, dried orange peel, and raw Pinellia tuber respectively to obtain a powder with a particle size of 0.5-100 mm, mix Atractylodes lancea, dried orange peel, Magnolia officinalis, and Angelica dahurica according to the above mass parts to obtain a mixed powder 1, add water 20 times the mass of the mixed powder 1, and boil at a temperature of 90° C. for 6.5 hours. During the boiling process, water is added to the initial boiling volume every half an hour; filter the decoction with a filter membrane with a pore size of 10 microns to obtain a filtrate and a filter residue; and concentrate the filtrate under reduced pressure at 62° C. to 0.7 times the mass of the mixed powder 1 to obtain a concentrate 1;

[0070] Step S3: Grind Atractylodes lancea, dried orange peel, Magnolia officinalis, and Angelica dahurica respectively to obtain a powder with a particle size of 0.5-100 mm, mix Poria cocos, dried orange peel, and raw Pinellia ternata according to the above mass parts to obtain mixed powder 2, then add a 55% ethanol aqueous solution with a volume concentration of 15 times the mass of the mixed powder 2 and the filter residue of step S2, stir at 80 rpm for 10 hours under the condition of heating to 58° C., filter through a filter membrane with a pore size of 10 microns, and concentrate the filtrate at 60° C. under reduced pressure to 0.8 times the mass of the mixed powder 2 to obtain concentrate 2;

[0071] Step S4: adding the concentrate 2 to an extractant consisting of an aqueous phase and an oil phase, extracting at a temperature of 48° C. for 4 hours, taking the extracted oil phase, and concentrating under reduced pressure at a temperature of 53° C. and a pressure of 0.025 MPa to remove cyclohexane and ethyl acetate, and stopping the reduced pressure concentration when the concentrate does not contain ethyl acetate to obtain a concentrate 3; wherein the mass ratio of the concentrate 2 to the extractant is 1:10; in the extractant, the aqueous phase is water, the oil phase is ethyl acetate, and the mass ratio of the aqueous phase to the oil phase is 1:0.8;

[0072] Step S5: combining the concentrate 1 of step S2, the concentrate 3 of step S4, licorice extract, patchouli oil, perilla leaf oil, polyglycerol-10 stearate, sucrose ester SE-15, water and ethanol to obtain a combined product, adjusting the total volume of the combined product to 2200 mL, stirring the combined product at a speed of 400 rpm for 2.5 hours to ensure uniform stirring, and filtering the combined product through a microporous filter with a pore size of 0.5 μm to obtain Huoxiang Zhengqi Water;

[0073] Among them, the total mass of polyglycerol-10 stearate and sucrose ester SE-15 accounts for 0.08% in Huoxiang Zhengqi water, the mass ratio of polyglycerol-10 stearate and sucrose ester SE-15 is 1:3, and the volume content of ethanol in Huoxiang Zhengqi water is 38%.

[0074] Huoxiang Zhengqi Water 5: The production process is as follows:

[0075] Step S1: prepare raw materials: 150 g of Atractylodes lancea, 160 g of dried orange peel, 160 g of Magnolia officinalis, 240 g of Angelica dahurica, 245 g of Poria, 250 g of Rhizoma Citri Reticulatae, 170 g of Raw Pinellia, 19 g of Licorice extract, 1.5 mL of Patchouli oil, 0.8 mL of Perilla leaf oil, polyglycerol-10 stearate, sucrose ester SE-15, ethanol, and water;

[0076] Step S2: grind Poria cocos, dried orange peel, and raw Pinellia tuber respectively to obtain a powder with a particle size of 0.5-100 mm, mix Atractylodes lancea, dried orange peel, Magnolia officinalis, and Angelica dahurica according to the above mass parts to obtain a mixed powder 1, add water 20 times the mass of the mixed powder 1, and boil at a temperature of 90° C. for 6.5 hours. During the boiling process, water is added to the initial boiling volume every half an hour; filter the decoction with a filter membrane with a pore size of 10 microns to obtain a filtrate and a filter residue; and concentrate the filtrate under reduced pressure at 62° C. to 0.7 times the mass of the mixed powder 1 to obtain a concentrate 1;

[0077] Step S3: Grind Atractylodes lancea, dried orange peel, Magnolia officinalis, and Angelica dahurica respectively to obtain a powder with a particle size of 0.5-100 mm, mix Poria cocos, dried orange peel, and raw Pinellia ternata according to the above mass parts to obtain mixed powder 2, then add a 55% ethanol aqueous solution with a volume concentration of 15 times the mass of the mixed powder 2 and the filter residue of step S2, stir at 80 rpm for 10 hours under the condition of heating to 58° C., filter through a filter membrane with a pore size of 10 microns, and concentrate the filtrate at 60° C. under reduced pressure to 0.8 times the mass of the mixed powder 2 to obtain concentrate 2;

[0078] Step S4: adding the concentrate 2 to an extractant consisting of an aqueous phase and an oil phase, extracting at a temperature of 48° C. for 4 hours, taking the extracted oil phase, and concentrating under reduced pressure at a temperature of 53° C. and a pressure of 0.025 MPa to remove cyclohexane and ethyl acetate, and stopping the reduced pressure concentration when the concentrate does not contain cyclohexane and ethyl acetate, to obtain a concentrate 3; wherein the mass ratio of the concentrate 2 to the extractant is 1:10; in the extractant, the aqueous phase is water, the oil phase is composed of cyclohexane and ethyl acetate in a mass ratio of 1:1, and the mass ratio of the aqueous phase to the oil phase is 1:0.8;

[0079] Step S5: combining the concentrate 1 of step S2, the concentrate 3 of step S4, licorice extract, patchouli oil, perilla leaf oil, polyglycerol-10 stearate, sucrose ester SE-15, water and ethanol to obtain a combined product, adjusting the total volume of the combined product to 2200 mL, stirring the combined product at a speed of 400 rpm for 2.5 hours to ensure uniform stirring, and filtering the combined product through a microporous filter with a pore size of 0.5 μm to obtain Huoxiang Zhengqi Water;

[0080] Among them, the total mass of polyglycerol-10 stearate and sucrose ester SE-15 accounts for 0.08% in Huoxiang Zhengqi water, the mass ratio of polyglycerol-10 stearate and sucrose ester SE-15 is 1:3, and the volume content of ethanol in Huoxiang Zhengqi water is 38%.

[0081] Huoxiang Zhengqi Water 6: The production process is as follows:

[0082] Step S1: prepare raw materials: 150 g of Atractylodes lancea, 160 g of dried orange peel, 160 g of Magnolia officinalis, 240 g of Angelica dahurica, 245 g of Poria, 250 g of Rhizoma Citri Reticulatae, 170 g of Raw Pinellia, 19 g of Licorice extract, 1.5 mL of Patchouli oil, 0.8 mL of Perilla leaf oil, polyglycerol-10 stearate, sucrose ester SE-15, ethanol, and water;

[0083] Step S2: grind Poria cocos, dried orange peel, and raw Pinellia tuber respectively to obtain a powder with a particle size of 0.5-100 mm, mix Atractylodes lancea, dried orange peel, Magnolia officinalis, and Angelica dahurica according to the above mass parts to obtain a mixed powder 1, add water 20 times the mass of the mixed powder 1, and boil at a temperature of 90° C. for 6.5 hours. During the boiling process, water is added to the initial boiling volume every half an hour; filter the decoction with a filter membrane with a pore size of 10 microns to obtain a filtrate and a filter residue; and concentrate the filtrate under reduced pressure at 62° C. to 0.7 times the mass of the mixed powder 1 to obtain a concentrate 1;

[0084] Step S3: Grind Atractylodes lancea, dried orange peel, Magnolia officinalis, and Angelica dahurica respectively to obtain a powder with a particle size of 0.5-100 mm, mix Poria cocos, dried orange peel, and raw Pinellia ternata according to the above mass parts to obtain mixed powder 2, then add a 55% ethanol aqueous solution with a volume concentration of 15 times the mass of the mixed powder 2 and the filter residue of step S2, stir at 80 rpm for 10 hours under the condition of heating to 58° C., filter through a filter membrane with a pore size of 10 microns, and concentrate the filtrate at 60° C. under reduced pressure to 0.8 times the mass of the mixed powder 2 to obtain concentrate 2;

[0085] Step S4: adding the concentrate 2 to an extractant consisting of an aqueous phase and an oil phase, extracting at a temperature of 48° C. for 4 hours, taking the extracted oil phase, and concentrating under reduced pressure at a temperature of 53° C. and a pressure of 0.025 MPa to remove cyclohexane and ethyl acetate, and stopping the reduced pressure concentration when the concentrate does not contain cyclohexane and ethyl acetate, to obtain a concentrate 3; wherein the mass ratio of the concentrate 2 to the extractant is 1:10; in the extractant, the aqueous phase is water, the oil phase is composed of cyclohexane and ethyl acetate in a mass ratio of 1:6, and the mass ratio of the aqueous phase to the oil phase is 1:0.8;

[0086] Step S5: combining the concentrate 1 of step S2, the concentrate 3 of step S4, licorice extract, patchouli oil, perilla leaf oil, polyglycerol-10 stearate, sucrose ester SE-15, water and ethanol to obtain a combined product, adjusting the total volume of the combined product to 2200 mL, stirring the combined product at a speed of 400 rpm for 2.5 hours to ensure uniform stirring, and filtering the combined product through a microporous filter with a pore size of 0.5 μm to obtain Huoxiang Zhengqi Water;

[0087] Among them, the total mass of polyglycerol-10 stearate and sucrose ester SE-15 accounts for 0.08% in Huoxiang Zhengqi water, the mass ratio of polyglycerol-10 stearate and sucrose ester SE-15 is 1:3, and the volume content of ethanol in Huoxiang Zhengqi water is 38%.

[0088] Huoxiang Zhengqi Water 7: The production process is as follows:

[0089] Step S1: prepare raw materials: 150 g of Atractylodes lancea, 160 g of dried orange peel, 160 g of Magnolia officinalis, 240 g of Angelica dahurica, 245 g of Poria, 250 g of Rhizoma Citri Reticulatae, 170 g of Raw Pinellia, 19 g of Licorice extract, 1.5 mL of Patchouli oil, 0.8 mL of Perilla leaf oil, polyglycerol-10 stearate, sucrose ester SE-15, ethanol, and water;

[0090] Step S2: grind Poria cocos, dried orange peel, and raw Pinellia tuber respectively to obtain a powder with a particle size of 0.5-100 mm, mix Atractylodes lancea, dried orange peel, Magnolia officinalis, and Angelica dahurica according to the above mass parts to obtain a mixed powder 1, add water 20 times the mass of the mixed powder 1, and boil at a temperature of 90° C. for 6.5 hours. During the boiling process, water is added to the initial boiling volume every half an hour; filter the decoction with a filter membrane with a pore size of 10 microns to obtain a filtrate and a filter residue; and concentrate the filtrate under reduced pressure at 62° C. to 0.7 times the mass of the mixed powder 1 to obtain a concentrate 1;

[0091] Step S3: Grind Atractylodes lancea, dried orange peel, Magnolia officinalis, and Angelica dahurica respectively to obtain a powder with a particle size of 0.5-100 mm, mix Poria cocos, dried orange peel, and raw Pinellia ternata according to the above mass parts to obtain mixed powder 2, then add a 55% ethanol aqueous solution with a volume concentration of 15 times the mass of the mixed powder 2 and the filter residue of step S2, stir at 80 rpm for 10 hours under the condition of heating to 58° C., filter through a filter membrane with a pore size of 10 microns, and concentrate the filtrate at 60° C. under reduced pressure to 0.8 times the mass of the mixed powder 2 to obtain concentrate 2;

[0092] Step S4: adding the concentrate 2 to an extractant consisting of an aqueous phase and an oil phase, extracting at a temperature of 48° C. for 4 hours, taking the extracted oil phase, and concentrating under reduced pressure at a temperature of 53° C. and a pressure of 0.025 MPa to remove cyclohexane and ethyl acetate, and stopping the reduced pressure concentration when the concentrate does not contain cyclohexane and ethyl acetate, to obtain a concentrate 3; wherein the mass ratio of the concentrate 2 to the extractant is 1:10; in the extractant, the aqueous phase is water, the oil phase is composed of cyclohexane and ethyl acetate in a mass ratio of 1:3, and the mass ratio of the aqueous phase to the oil phase is 1:0.8;

[0093] Step S5: combining the concentrate 1 of step S2, the concentrate 3 of step S4, licorice extract, patchouli oil, perilla leaf oil, polyglycerol-10 stearate, sucrose ester SE-15, water and ethanol to obtain a combined product, adjusting the total volume of the combined product to 2200 mL, stirring the combined product at a speed of 400 rpm for 2.5 hours to ensure uniform stirring, and filtering the combined product through a microporous filter with a pore size of 0.5 μm to obtain Huoxiang Zhengqi Water;

[0094] Among them, the total mass of polyglycerol-10 stearate and sucrose ester SE-15 in Huoxiang Zhengqi water accounts for 0.08%, the mass ratio of polyglycerol-10 stearate and sucrose ester SE-15 is 1:0.5, and the volume content of ethanol in Huoxiang Zhengqi water is 38%.

[0095] Huoxiang Zhengqi Water 8: The production process is as follows:

[0096] Step S1: prepare raw materials: 150 g of Atractylodes lancea, 160 g of dried orange peel, 160 g of Magnolia officinalis, 240 g of Angelica dahurica, 245 g of Poria, 250 g of Rhizoma Citri Reticulatae, 170 g of Raw Pinellia, 19 g of Licorice extract, 1.5 mL of Patchouli oil, 0.8 mL of Perilla leaf oil, polyglycerol-10 stearate, sucrose ester SE-15, ethanol, and water;

[0097] Step S2: grind Poria cocos, dried orange peel, and raw Pinellia tuber respectively to obtain a powder with a particle size of 0.5-100 mm, mix Atractylodes lancea, dried orange peel, Magnolia officinalis, and Angelica dahurica according to the above mass parts to obtain a mixed powder 1, add water 20 times the mass of the mixed powder 1, and boil at a temperature of 90° C. for 6.5 hours. During the boiling process, water is added to the initial boiling volume every half an hour; filter the decoction with a filter membrane with a pore size of 10 microns to obtain a filtrate and a filter residue; and concentrate the filtrate under reduced pressure at 62° C. to 0.7 times the mass of the mixed powder 1 to obtain a concentrate 1;

[0098] Step S3: Grind Atractylodes lancea, dried orange peel, Magnolia officinalis, and Angelica dahurica respectively to obtain a powder with a particle size of 0.5-100 mm, mix Poria cocos, dried orange peel, and raw Pinellia ternata according to the above mass parts to obtain mixed powder 2, then add a 55% ethanol aqueous solution with a volume concentration of 15 times the mass of the mixed powder 2 and the filter residue of step S2, stir at 80 rpm for 10 hours under the condition of heating to 58° C., filter through a filter membrane with a pore size of 10 microns, and concentrate the filtrate at 60° C. under reduced pressure to 0.8 times the mass of the mixed powder 2 to obtain concentrate 2;

[0099] Step S4: adding the concentrate 2 to an extractant consisting of an aqueous phase and an oil phase, extracting at a temperature of 48° C. for 4 hours, taking the extracted oil phase, and concentrating under reduced pressure at a temperature of 53° C. and a pressure of 0.025 MPa to remove cyclohexane and ethyl acetate, and stopping the reduced pressure concentration when the concentrate does not contain cyclohexane and ethyl acetate, to obtain a concentrate 3; wherein the mass ratio of the concentrate 2 to the extractant is 1:10; in the extractant, the aqueous phase is water, the oil phase is composed of cyclohexane and ethyl acetate in a mass ratio of 1:3, and the mass ratio of the aqueous phase to the oil phase is 1:0.8;

[0100] Step S5: combining the concentrate 1 of step S2, the concentrate 3 of step S4, licorice extract, patchouli oil, perilla leaf oil, polyglycerol-10 stearate, sucrose ester SE-15, water and ethanol to obtain a combined product, adjusting the total volume of the combined product to 2200 mL, stirring the combined product at a speed of 400 rpm for 2.5 hours to ensure uniform stirring, and filtering the combined product through a microporous filter with a pore size of 0.5 μm to obtain Huoxiang Zhengqi Water;

[0101] Among them, the total mass of polyglycerol-10 stearate and sucrose ester SE-15 in Huoxiang Zhengqi water accounts for 0.08%, the mass ratio of polyglycerol-10 stearate and sucrose ester SE-15 is 1:1, and the volume content of ethanol in Huoxiang Zhengqi water is 38%.

[0102] Huoxiang Zhengqi Water 9: The production process is as follows:

[0103] Step S1: prepare raw materials: 150 g of Atractylodes lancea, 160 g of dried orange peel, 160 g of Magnolia officinalis, 240 g of Angelica dahurica, 245 g of Poria, 250 g of Rhizoma Citri Reticulatae, 170 g of Raw Pinellia, 19 g of Licorice extract, 1.5 mL of Patchouli oil, 0.8 mL of Perilla leaf oil, polyglycerol-10 stearate, sucrose ester SE-15, ethanol, and water;

[0104] Step S2: grind Poria cocos, dried orange peel, and raw Pinellia tuber respectively to obtain a powder with a particle size of 0.5-100 mm, mix Atractylodes lancea, dried orange peel, Magnolia officinalis, and Angelica dahurica according to the above mass parts to obtain a mixed powder 1, add water 20 times the mass of the mixed powder 1, and boil at a temperature of 90° C. for 6.5 hours. During the boiling process, water is added to the initial boiling volume every half an hour; filter the decoction with a filter membrane with a pore size of 10 microns to obtain a filtrate and a filter residue; and concentrate the filtrate under reduced pressure at 62° C. to 0.7 times the mass of the mixed powder 1 to obtain a concentrate 1;

[0105] Step S3: Grind Atractylodes lancea, dried orange peel, Magnolia officinalis, and Angelica dahurica respectively to obtain a powder with a particle size of 0.5-100 mm, mix Poria cocos, dried orange peel, and raw Pinellia ternata according to the above mass parts to obtain mixed powder 2, then add a 55% ethanol aqueous solution with a volume concentration of 15 times the mass of the mixed powder 2 and the filter residue of step S2, stir at 80 rpm for 10 hours under the condition of heating to 58° C., filter through a filter membrane with a pore size of 10 microns, and concentrate the filtrate at 60° C. under reduced pressure to 0.8 times the mass of the mixed powder 2 to obtain concentrate 2;

[0106] Step S4: adding the concentrate 2 to an extractant consisting of an aqueous phase and an oil phase, extracting at a temperature of 48° C. for 4 hours, taking the extracted oil phase, and concentrating under reduced pressure at a temperature of 53° C. and a pressure of 0.025 MPa to remove cyclohexane and ethyl acetate, and stopping the reduced pressure concentration when the concentrate does not contain cyclohexane and ethyl acetate, to obtain a concentrate 3; wherein the mass ratio of the concentrate 2 to the extractant is 1:10; in the extractant, the aqueous phase is water, the oil phase is composed of cyclohexane and ethyl acetate in a mass ratio of 1:3, and the mass ratio of the aqueous phase to the oil phase is 1:0.8;

[0107] Step S5: combining the concentrate 1 of step S2, the concentrate 3 of step S4, licorice extract, patchouli oil, perilla leaf oil, polyglycerol-10 stearate, sucrose ester SE-15, water and ethanol to obtain a combined product, adjusting the total volume of the combined product to 2200 mL, stirring the combined product at a speed of 400 rpm for 2.5 hours to ensure uniform stirring, and filtering the combined product through a microporous filter with a pore size of 0.5 μm to obtain Huoxiang Zhengqi Water;

[0108] Among them, the total mass of polyglycerol-10 stearate and sucrose ester SE-15 in Huoxiang Zhengqi water accounts for 0.08%, the mass ratio of polyglycerol-10 stearate and sucrose ester SE-15 is 1:5.5, and the volume content of ethanol in Huoxiang Zhengqi water is 38%.

[0109] Huoxiang Zhengqi Water 10: The production process is as follows:

[0110] Step S1: prepare raw materials: 150 g of Atractylodes lancea, 160 g of dried orange peel, 160 g of Magnolia officinalis, 240 g of Angelica dahurica, 245 g of Poria, 250 g of Rhizoma Citri Reticulatae, 170 g of Raw Pinellia, 19 g of Licorice extract, 1.5 mL of Patchouli oil, 0.8 mL of Perilla leaf oil, polyglycerol-10 stearate, sucrose ester SE-15, ethanol, and water;

[0111] Step S2: grind Poria cocos, dried orange peel, and raw Pinellia tuber respectively to obtain a powder with a particle size of 0.5-100 mm, mix Atractylodes lancea, dried orange peel, Magnolia officinalis, and Angelica dahurica according to the above mass parts to obtain a mixed powder 1, add water 20 times the mass of the mixed powder 1, and boil at a temperature of 90° C. for 6.5 hours. During the boiling process, water is added to the initial boiling volume every half an hour; filter the decoction with a filter membrane with a pore size of 10 microns to obtain a filtrate and a filter residue; and concentrate the filtrate under reduced pressure at 62° C. to 0.7 times the mass of the mixed powder 1 to obtain a concentrate 1;

[0112] Step S3: Grind Atractylodes lancea, dried orange peel, Magnolia officinalis, and Angelica dahurica respectively to obtain a powder with a particle size of 0.5-100 mm, mix Poria cocos, dried orange peel, and raw Pinellia ternata according to the above mass parts to obtain mixed powder 2, then add a 55% ethanol aqueous solution with a volume concentration of 15 times the mass of the mixed powder 2 and the filter residue of step S2, stir at 80 rpm for 10 hours under the condition of heating to 58° C., filter through a filter membrane with a pore size of 10 microns, and concentrate the filtrate at 60° C. under reduced pressure to 0.8 times the mass of the mixed powder 2 to obtain concentrate 2;

[0113] Step S4: adding the concentrate 2 to an extractant consisting of an aqueous phase and an oil phase, extracting at a temperature of 48° C. for 4 hours, taking the extracted oil phase, and concentrating under reduced pressure at a temperature of 53° C. and a pressure of 0.025 MPa to remove cyclohexane and ethyl acetate, and stopping the reduced pressure concentration when the concentrate does not contain cyclohexane and ethyl acetate, to obtain a concentrate 3; wherein the mass ratio of the concentrate 2 to the extractant is 1:10; in the extractant, the aqueous phase is water, the oil phase is composed of cyclohexane and ethyl acetate in a mass ratio of 1:3, and the mass ratio of the aqueous phase to the oil phase is 1:0.8;

[0114] Step S5: combining the concentrate 1 of step S2, the concentrate 3 of step S4, licorice extract, patchouli oil, perilla leaf oil, polyglycerol-10 stearate, sucrose ester SE-15, water and ethanol to obtain a combined product, adjusting the total volume of the combined product to 2200 mL, stirring the combined product at a speed of 400 rpm for 2.5 hours to ensure uniform stirring, and filtering the combined product through a microporous filter with a pore size of 0.5 μm to obtain Huoxiang Zhengqi Water;

[0115] Among them, the total mass of polyglycerol-10 stearate and sucrose ester SE-15 accounts for 0.08% in Huoxiang Zhengqi water, the mass ratio of polyglycerol-10 stearate and sucrose ester SE-15 is 1:7, and the volume content of ethanol in Huoxiang Zhengqi water is 38%.

[0116] Huoxiang Zhengqi Water 11: The production process is as follows:

[0117] Step S1: prepare raw materials: 150 g of Atractylodes lancea, 160 g of dried orange peel, 160 g of Magnolia officinalis, 240 g of Angelica dahurica, 245 g of Poria, 250 g of Rhizoma Citri Reticulatae, 170 g of Raw Pinellia, 19 g of Licorice extract, 1.5 mL of Patchouli oil, 0.8 mL of Perilla leaf oil, Tween-60, ethanol, and water;

[0118] Step S2: grind Poria cocos, dried orange peel, and raw Pinellia tuber respectively to obtain a powder with a particle size of 0.5-100 mm, mix Atractylodes lancea, dried orange peel, Magnolia officinalis, and Angelica dahurica according to the above mass parts to obtain a mixed powder 1, add water 20 times the mass of the mixed powder 1, and boil at a temperature of 90° C. for 6.5 hours. During the boiling process, water is added to the initial boiling volume every half an hour; filter the decoction with a filter membrane with a pore size of 10 microns to obtain a filtrate and a filter residue; and concentrate the filtrate under reduced pressure at 62° C. to 0.7 times the mass of the mixed powder 1 to obtain a concentrate 1;

[0119] Step S3: Grind Atractylodes lancea, dried orange peel, Magnolia officinalis, and Angelica dahurica respectively to obtain a powder with a particle size of 0.5-100 mm, mix Poria cocos, dried orange peel, and raw Pinellia ternata according to the above mass parts to obtain mixed powder 2, then add a 55% ethanol aqueous solution with a volume concentration of 15 times the mass of the mixed powder 2 and the filter residue of step S2, stir at 80 rpm for 10 hours under the condition of heating to 58° C., filter through a filter membrane with a pore size of 10 microns, and concentrate the filtrate at 60° C. under reduced pressure to 0.8 times the mass of the mixed powder 2 to obtain concentrate 2;

[0120] Step S4: adding the concentrate 2 to an extractant consisting of an aqueous phase and an oil phase, extracting at a temperature of 48° C. for 4 hours, taking the extracted oil phase, and concentrating under reduced pressure at a temperature of 53° C. and a pressure of 0.025 MPa to remove cyclohexane and ethyl acetate, and stopping the reduced pressure concentration when the concentrate does not contain cyclohexane and ethyl acetate, to obtain a concentrate 3; wherein the mass ratio of the concentrate 2 to the extractant is 1:10; in the extractant, the aqueous phase is water, the oil phase is composed of cyclohexane and ethyl acetate in a mass ratio of 1:3, and the mass ratio of the aqueous phase to the oil phase is 1:0.8;

[0121] Step S5: combining the concentrate 1 of step S2, the concentrate 3 of step S4, licorice extract, patchouli oil, perilla leaf oil, Tween-60, water and ethanol to obtain a combined product, adjusting the total volume of the combined product to 2200 mL, stirring the combined product at a speed of 400 rpm for 2.5 hours to ensure uniform stirring, and filtering the combined product through a 0.5 μm pore size microporous filter to obtain Huoxiang Zhengqi Water;

[0122] Among them, the mass content of Tween-60 in Huoxiang Zhengqi water is 0.08%, and the volume content of ethanol in Huoxiang Zhengqi water is 38%.

[0123] 2. Performance Test

[0124] The above-mentioned Huoxiang Zhengqi water was subjected to the following performance tests.

[0125] 1. Antidiarrhea effect test

[0126] The antidiarrheal effect of the Huoxiang Zhengqi water of the present invention was verified by evaluating the antidiarrheal effect on senna leaf diarrhea model rats.

[0127] A number of healthy clean Wi star male rats weighing 150±10 g were taken and raised normally for 7 days; the rats were randomly divided into 9 groups according to their weight, namely, normal group, treatment group 1-6, control group and model group, with 10 rats in each group. Among them, treatment group 1-6 is a treatment group that uses the above-mentioned Huoxiang Zhengqi Water 1-6 in sequence, also known as Huoxiang Zhengqi Water 1-6 group.

[0128] The diarrhea model was induced by senna leaves in rats: except for the normal group, rats in other groups were gavaged with senna leaf concentrate; the gavage volume was 4ml / 150g / time / day, and the gavage was continued for 7 consecutive days, once a day, to create a diarrhea model. The normal group was gavaged with an equal amount of normal saline in the same way for 7 days.

[0129] Drug administration: After modeling, the treatment groups 1-6 were gavaged with the above-mentioned Huoxiang Zhengqi Water 1-6, with a dosage of 4 mL / 150 g / time / day, for 3 consecutive days, once a day. After modeling, the control group was gavaged with berberine hydrochloride solution (concentration of 0.2 g / ml), with a dosage of 3.5 ml / 150 g / time / day, for 3 consecutive days, once a day. The model group and the normal group were gavaged with an equal amount of normal saline in the same way, once a day for 3 consecutive days.

[0130] The number of loose stools, total number of stools, and loose stool grade of rats in each group were observed within 6 hours after the last administration.

[0131] Among them, the loose stool grade indicates the degree of loose stool. It is graded according to the size of the stain area formed by the loose stool contaminating the filter paper. It is divided into 4 levels and judged according to the standards in Table 1.

[0132] Table 1 Judgment criteria for loose stool grade

[0133] series 1 2 3 4 Diameter / cm ﹤1 1-1.9 2-3 ﹥3

[0134] The process of counting and calculating the diarrhea index is as follows: Calculate the loose stool rate, which represents the ratio of the number of loose stools discharged by each animal to the total number of stools. When counting, first count the level of each pile of loose stools one by one according to Table 1, then add up all the loose stool levels of the mouse and divide by the number of loose stools to get the average level of loose stools, referred to as the loose stool level. Diarrhea index: the product of loose stool rate and loose stool level. The specific diarrhea index average value (diarrhea index value) of each group is shown in Table 2.

[0135] Table 2 Diarrhea index of each test group

[0136]

[0137]

[0138] According to the test results in Table 2, the Huoxiang Zhengqi water obtained by the present invention showed a diarrhea index value of 0.79±0.09 when the antidiarrhea test was carried out on rats with senna diarrhea model. The Huoxiang Zhengqi water obtained by the present invention can effectively inhibit diarrhea.

[0139] By comparing the 1st group of Huoxiang Zhengqi water with the 3-6 groups, it can be known that the present invention uses a composite extractant extraction process to more efficiently extract the active ingredients in the filter residues of Atractylodes lancea, Tangerine peel, Magnolia bark, Angelica dahurica, Poria cocos, Cortex tangerines, and Raw Pinellia tuber, so that the types and contents of effective ingredients in Huoxiang Zhengqi water are more abundant, and the antidiarrheal effect of Huoxiang Zhengqi water is increased. The use of cyclohexane and ethyl acetate in the composite extractant of the present invention can produce a mutually reinforcing relationship, and the two promote synergistically to increase the antidiarrheal effect of Huoxiang Zhengqi water, which can be obtained by comparing the 1st group of Huoxiang Zhengqi water with the 3-4 groups. And when the mass ratio of cyclohexane to ethyl acetate in the extractant is 1:2.5-4, Huoxiang Zhengqi water shows a better antidiarrheal effect, which can be specifically obtained by the test results of the 1-2 group and the 5-6 group.

[0140] 2. Stability test

[0141] Zeta potential is used to characterize the stability of the Huoxiang Zhengqi water obtained by the present invention. The conductivity of the Huoxiang Zhengqi water prepared above is adjusted to 50S / cm using a diluting solvent, wherein the diluting solvent consists of ethanol, sodium chloride and deionized water, the mass concentration of ethanol is 35%, and the mass concentration of sodium chloride is 0.9%; then stirred at a speed of 600 rpm for 1.5 hours to obtain a test solution, and the Zeta potential is tested to obtain the Zeta potential of the Huoxiang Zhengqi water. The test results are shown in Table 3.

[0142] Table 3 Zeta potential of Huoxiang Zhengqi water

[0143] test Zeta potential / mV Huoxiang Zhengqi Water 1 set -38.47 Huoxiang Zhengqi Water 3 Groups -38.12 Huoxiang Zhengqi Water 4 Groups -37.97 Huoxiang Zhengqi Water 5 Groups -38.01 Huoxiang Zhengqi Water 6 Groups -37.78 Huoxiang Zhengqi Water 7 Groups -32.16 Huoxiang Zhengqi Water 8 Groups -34.62 Huoxiang Zhengqi Water 9 Groups -35.51 Huoxiang Zhengqi Water 10 sets -33.03 Huoxiang Zhengqi Water 11 Group -29.45

[0144] After the test solution was placed at 40°C for 60 days, the Zeta potential was tested again to obtain the Zeta potential of the Huoxiang Zhengqi water after storage. The results are shown in Table 4.

[0145] Table 4 Zeta potential of Huoxiang Zhengqi water after storage

[0146] test Zeta potential after storage Huoxiang Zhengqi Water 1 set -30.52 Huoxiang Zhengqi Water 3 Groups -30.12 Huoxiang Zhengqi Water 4 Groups -29.89 Huoxiang Zhengqi Water 5 Groups -30.03 Huoxiang Zhengqi Water 6 Groups -29.80 Huoxiang Zhengqi Water 7 Groups -23.62 Huoxiang Zhengqi Water 8 Groups -26.31 Huoxiang Zhengqi Water 9 Groups -27.29 Huoxiang Zhengqi Water 10 sets -24.57 Huoxiang Zhengqi Water 11 Group -20.64

[0147] From the test results in Table 3-4, it can be seen that the Huoxiang Zhengqi water obtained by the present invention is diluted with a dilution solvent and the conductivity is adjusted to 50S / cm, and the conductivity is -38.47mV. The Huoxiang Zhengqi water of the present invention has good stability. And after it is stored at a high temperature of 40°C for 60 days, its conductivity is -30.52mV, which still has good stability, indicating that the Huoxiang Zhengqi water prepared by the present invention still maintains a good stability effect after being affected by an adverse environment.

[0148] From the comparison of Huoxiang Zhengqi Water Group 1-2 and Group 8-11, it can be obtained that the addition of polyglycerol-10 stearate and sucrose ester SE-15 in the Huoxiang Zhengqi Water of the present invention can give the product better stability. The combination of the two makes Huoxiang Zhengqi Water have a higher absolute value of Zeta potential after storage at room temperature and high temperature, giving the product excellent stability. In the preparation system of the present invention, especially when the mass ratio of polyglycerol-10 stearate and sucrose ester SE-15 is 1:2-4, the stability of Huoxiang Zhengqi Water shows a more favorable change trend. In the preparation system of the present invention, after using other materials, such as Tween-60, to replace the combination of polyglycerol-10 stearate and sucrose ester SE-15, the stability of the obtained Huoxiang Zhengqi Water shows an unfavorable decrease, which cannot meet the requirements of container storage, etc., so that its use effect is reduced.

[0149] It can be seen from the contents of Table 3 that the composite extraction process used in the present invention has a certain influence on the stability of the Huoxiang Zhengqi water finally prepared.

[0150] The invention boils Poria cocos, dried orange peel and raw Pinellia tuber, obtains filtrate and filter residue by filtering, and concentrates the filtrate to obtain a concentrate; extracts Atractylodes lancea, dried orange peel, Magnolia officinalis, Angelica dahurica and the filter residue simultaneously with an ethanol aqueous solution, and concentrates, and then extracts efficiently with an extractant composed of an aqueous phase and an oil phase, and removes the extraction solvent by concentration to obtain a concentrate; and then prepares Huoxiang Zhengqi water by combining different concentrates with liquorice extract, patchouli oil, perilla leaf oil, polyglycerol-10 stearate, sucrose ester SE-15, water and ethanol, wherein the ethanol content in the Huoxiang Zhengqi water is as low as 35-45%, the water still has excellent stability, and the antidiarrheal effect is excellent.

[0151] Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include these modifications and variations.

Claims

1. A method for preparing Huoxiang Zhengqi water, It is characterized in that The steps include: Step S1: prepare raw materials: 145-165 g of Atractylodes lancea, 140-170 g of dried orange peel, 150-170 g of Magnolia officinalis, 230-260 g of Angelica dahurica, 230-260 g of Poria cocos, 230-260 g of Rhizoma Cibotii, 150-180 g of Raw Pinellia, 17-21 g of Licorice extract, 1.4-1.7 mL of Patchouli oil, 0.7-1 mL of Perilla leaf oil, polyglycerol-10 stearate, sucrose ester SE-15, ethanol, and water; Step S2: grind Poria cocos, dried tangerine peel, and raw Pinellia tuber to obtain powder with a particle size of 0.5-100 mm, mix Atractylodes lancea, dried orange peel, Magnolia officinalis, and Angelica dahurica according to the above mass parts to obtain mixed powder 1, add water to boil, filter, and obtain a filtrate and a filter residue; concentrate the filtrate to obtain a concentrate 1; Step S3: Grind Atractylodes lancea, dried orange peel, Magnolia officinalis, and Angelica dahurica respectively to obtain a powder with a particle size of 0.5-100 mm, mix Poria cocos, dried orange peel, and raw Pinellia ternata according to the above mass parts to obtain a mixed powder 2, then add ethanol aqueous solution and the filter residue of step S2, stir under heating, filter, and concentrate the filtrate to obtain a concentrate 2; wherein, in the extractant, the aqueous phase is water, and the oil phase is composed of cyclohexane and ethyl acetate in a mass ratio of 1:2.5-4; Step S4: adding the concentrate 2 to an extractant consisting of an aqueous phase and an oil phase for extraction, taking the extracted oil phase, and concentrating under reduced pressure to remove cyclohexane and ethyl acetate to obtain a concentrate 3; Step S5: combining the concentrate 1 of step S2, the concentrate 3 of step S4, licorice extract, patchouli oil, perilla leaf oil, polyglycerol-10 stearate, sucrose ester SE-15, water and ethanol to obtain a combined product, adjusting the total volume of the combined product to 1800-2500 mL, then stirring evenly, and microfiltration to obtain Huoxiang Zhengqi water; Among them, the volume content of ethanol in Huoxiang Zhengqi water is 35-45%; The mass ratio of polyglycerol-10 stearate to sucrose ester SE-15 is 1:2-4; The total mass of polyglycerol-10 stearate and sucrose ester SE-15 accounts for 0.04-0.08% in Huoxiang Zhengqi water; In step S4, the mass ratio of the concentrate 2 to the extractant is 1:5-50, and the mass ratio of the water phase to the oil phase is 1:0.5-1.5; In step S4, the extraction temperature is 40-55° C. and the extraction time is 2-7 hours; In step S3, the volume concentration of the ethanol aqueous solution is 55%; In step S3 , the concentrate 2 is 0.5-1.5 times the mass of the mixed powder 2 .

2. The preparation method according to claim 1, It is characterized in that The mass ratio of polyglycerol-10 stearate to sucrose ester SE-15 is 1:2.5-3.

5.

3. The preparation method according to claim 1, It is characterized in that The oil phase is composed of cyclohexane and ethyl acetate in a mass ratio of 1:3-4.

4. The preparation method according to claim 1, It is characterized in that In step S3, the mass ratio of the mixed powder 2 to the ethanol aqueous solution is 1:8-50.

5. Huoxiang Zhengqi water obtained by the preparation method according to any one of claims 1 to 4.

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