A Chinese medicine composition and its preparation method and its application in preparing medicine for treating thalassemia

Abstract

The present invention relates to the technical field of traditional Chinese medicine, and specifically discloses a traditional Chinese medicine composition and a preparation method thereof and an application thereof in preparing a medicine for treating thalassemia. The traditional Chinese medicine composition is made of the following raw material components: astragalus, wolfberry, epimedium and millettia reticulata. Studies have shown that the traditional Chinese medicine composition of the present invention can effectively increase the number of red blood cells and / or the hemoglobin content in the blood; thus, it can be seen that the traditional Chinese medicine composition of the present invention can play a role in treating anemia by increasing the number of red blood cells and / or the hemoglobin content in the blood, especially playing a role in treating thalassemia.

Description

Technical Field

[0001] The present invention relates to the technical field of traditional Chinese medicines, and in particular to a traditional Chinese medicine composition and a preparation method thereof, and application of the traditional Chinese medicine composition in preparing a medicine for treating thalassemia. Background Art

[0002] Anemia is a common blood disease, which is mainly caused by a decrease in the number of red blood cells in the peripheral blood, resulting in insufficient oxygen supply to tissues and organs; which in turn causes symptoms including headaches, tinnitus, syncope, muscle weakness, shortness of breath, palpitations, digestive discomfort, menstrual disorders, sexual dysfunction, etc. Among them, poor eating habits, inflammation, chronic diseases or malignant tumors can all lead to the occurrence of anemia. Thalassemia, also known as thalassemia, is a genetic disease with anemia as the main manifestation; patients with thalassemia need long-term hemoglobin maintenance treatment; in severe cases, blood transfusion therapy is required.

[0003] Therefore, developing a traditional Chinese medicine that can increase the number of red blood cells and the hemoglobin content in the blood has important application value for anemia patients, especially patients with thalassemia. Summary of the invention

[0004] In order to overcome at least one of the technical problems existing in the prior art, the present invention provides a traditional Chinese medicine composition.

[0005] The above technical problems to be solved by the present invention are achieved through the following technical solutions:

[0006] The present invention firstly provides a traditional Chinese medicine composition, which is prepared from the following raw material components: astragalus, wolfberry, epimedium and millettia reticulata.

[0007] The inventors found in their research that the Chinese medicine composition prepared with the Chinese medicines Astragalus, Lycium barbarum, Epimedium and Millettia reticulata as raw materials can effectively increase the number of red blood cells and / or the hemoglobin content in the blood; it can be seen that the Chinese medicine composition of the present invention can play a role in treating anemia by increasing the number of red blood cells and / or the hemoglobin content in the blood, especially playing a role in treating thalassemia.

[0008] Preferably, the weight proportions of astragalus, wolfberry, epimedium and millettia reticulata in the traditional Chinese medicine composition are respectively:

[0009] 25-40 parts of Astragalus; 20-30 parts of Lycium barbarum; 10-20 parts of Millettia reticulata; 8-15 parts of Epimedium.

[0010] Preferably, the weight proportions of astragalus, wolfberry, epimedium and millettia reticulata in the traditional Chinese medicine composition are respectively:

[0011] 30 parts of Astragalus; 25 parts of Lycium barbarum; 15 parts of Millettia spatholobi; 10 parts of Epimedium.

[0012] Preferably, saffron and burdock root are also added to the raw material components of the traditional Chinese medicine composition.

[0013] The traditional Chinese medicine composition is prepared from the following raw material components: astragalus, wolfberry, epimedium, millettia reticulata, saffron and burdock root.

[0014] Preferably, the weight proportions of astragalus, wolfberry, epimedium, millettia reticulata, saffron and burdock root in the traditional Chinese medicine composition are respectively:

[0015] 25-40 parts of astragalus; 20-30 parts of wolfberry; 10-20 parts of Millettia reticulata; 8-15 parts of epimedium; 20-30 parts of saffron; 10-20 parts of burdock root.

[0016] Preferably, the weight proportions of astragalus, wolfberry, epimedium, millettia reticulata, saffron and burdock root in the traditional Chinese medicine composition are respectively:

[0017] 30 parts of Astragalus; 25 parts of Lycium barbarum; 15 parts of Millettia spatholobi; 10 parts of Epimedium; 25 parts of Saffron; 15 parts of Burdock Root.

[0018] The inventors surprisingly found in further research that saffron and burdock root were further added to the four Chinese medicinal herbs of astragalus, wolfberry, epimedium and Millettia spatholobi; the Chinese medicinal composition prepared with the six Chinese medicinal herbs of astragalus, wolfberry, epimedium, Millettia spatholobi, saffron and burdock root as raw materials has a significantly higher effect on increasing the number of red blood cells and / or hemoglobin content in the blood than the Chinese medicinal composition prepared with the four Chinese medicinal herbs of astragalus, wolfberry, epimedium and Millettia spatholobi as raw materials; the Chinese medicinal composition can have a better effect on treating anemia or thalassemia.

[0019] However, the inventors need to explain that saffron and burdock root must be further added to the four Chinese medicinal herbs of astragalus, wolfberry, epimedium and Millettia spatholobi at the same time; the Chinese medicine composition prepared with six Chinese medicinal herbs of astragalus, wolfberry, epimedium, Millettia spatholobi, saffron and burdock root as raw materials can significantly improve the effect of the Chinese medicine composition prepared with four Chinese medicinal herbs of astragalus, wolfberry, epimedium and Millettia spatholobi as raw materials on increasing the number of red blood cells and / or hemoglobin content in the blood; however, the Chinese medicine composition prepared by only adding saffron or burdock root to the four Chinese medicinal herbs of astragalus, wolfberry, epimedium and Millettia spatholobi cannot significantly improve the effect of the Chinese medicine composition prepared with four Chinese medicinal herbs of astragalus, wolfberry, epimedium and Millettia spatholobi as raw materials on increasing the number of red blood cells and / or hemoglobin content in the blood.

[0020] The present invention also provides a method for preparing a Chinese medicine composition, characterized in that it comprises the following steps:

[0021] The raw material components are mixed and heated to boiling state with a solvent, and then reflux extraction is performed for 30 to 120 minutes. After the extraction is completed, the extract is concentrated and dried to obtain the traditional Chinese medicine composition.

[0022] Preferably, the weight amount of the solvent is 10 to 20 times the total weight of the raw materials.

[0023] Preferably, the solvent is an ethanol aqueous solution.

[0024] Preferably, the ethanol aqueous solution refers to an ethanol aqueous solution with an ethanol volume fraction of 50 to 80%.

[0025] More preferably, the solvent used is a mixed solvent consisting of ethanol aqueous solution, acetone and ethyl acetate.

[0026] Preferably, the volume ratio of the ethanol aqueous solution to acetone and ethyl acetate in the mixed solvent is 3-6:1-3:1-3.

[0027] Most preferably, the volume ratio of ethanol aqueous solution to acetone and ethyl acetate in the mixed solvent is 5:2:2.

[0028] Preferably, the ethanol aqueous solution refers to an ethanol aqueous solution with an ethanol volume fraction of 50 to 80%.

[0029] The inventors found in the study that the Chinese medicine composition prepared by using different extraction solvents in the preparation process of the Chinese medicine composition of the present invention has different effects on increasing the number of red blood cells and / or hemoglobin content in the blood. The inventors surprisingly found in the study that the Chinese medicine composition prepared by using six Chinese medicines such as astragalus, wolfberry, epimedium, Millettia reticulata, saffron and burdock root as raw materials and using a mixed solvent consisting of ethanol aqueous solution, acetone and ethyl acetate has a much higher effect on increasing the number of red blood cells and / or hemoglobin content in the blood than the Chinese medicine composition prepared by using conventional ethanol aqueous solution.

[0030] In addition, the inventors also found that the effect of increasing the number of red blood cells and / or hemoglobin content in the blood by using a mixed solvent consisting of an ethanol aqueous solution, acetone and ethyl acetate as raw materials for the Chinese medicine composition prepared is significantly higher than that of the Chinese medicine composition prepared by using a conventional ethanol aqueous solution; however, the effect of increasing the number of red blood cells and / or hemoglobin content in the blood by using a combination of other solvents is not significantly higher than that of the Chinese medicine composition prepared by using a conventional ethanol aqueous solution.

[0031] The present invention also provides an application of the above-mentioned traditional Chinese medicine composition in preparing a medicine having the function of increasing the number of red blood cells and / or the content of hemoglobin in blood.

[0032] The present invention also provides an application of the traditional Chinese medicine composition in preparing a medicine for treating anemia.

[0033] The present invention also provides an application of the traditional Chinese medicine composition in preparing a medicine for treating thalassemia.

[0034] Beneficial effects: The present invention provides a Chinese medicine composition with a new composition, which is prepared with Chinese medicines astragalus, wolfberry, epimedium and Millettia reticulata as raw materials; studies have shown that it can effectively increase the number of red blood cells and / or the hemoglobin content in the blood; thus, it can be seen that the Chinese medicine composition of the present invention can play a role in treating anemia by increasing the number of red blood cells and / or the hemoglobin content in the blood, especially playing a role in treating thalassemia. DETAILED DESCRIPTION

[0035] The present invention is further explained below with reference to the examples, but the examples do not limit the present invention in any form.

[0036] Example 1 Preparation of Chinese medicine composition

[0037] The Chinese medicinal raw materials are composed by weight: 30 parts of Astragalus; 25 parts of Lycium barbarum; 15 parts of Millettia reticulata; 10 parts of Epimedium;

[0038] The preparation method of the Chinese medicine composition comprises: mixing astragalus, wolfberry, epimedium and millettia reticulata, crushing the mixture and passing through a 100-mesh sieve, then heating the mixture to boiling state with a solvent of 12 times the total weight of the Chinese medicine raw materials, and then reflux extracting the mixture for 90 minutes. After the extraction, the extract is concentrated and dried to obtain the Chinese medicine composition.

[0039] The solvent used is an ethanol aqueous solution with an ethanol volume fraction of 75%.

[0040] Example 2 Preparation of Chinese medicine composition

[0041] The Chinese medicinal raw materials are composed by weight: 30 parts of astragalus; 25 parts of wolfberry; 15 parts of Millettia reticulata; 10 parts of epimedium; 25 parts of saffron; 15 parts of burdock root;

[0042] The preparation method of the Chinese medicine composition comprises: mixing astragalus, wolfberry, epimedium, millettia reticulata, saffron and burdock root, crushing the mixture and passing through a 100-mesh sieve, then heating the mixture to boiling state with a solvent of 12 times the total weight of the Chinese medicine raw materials, and then reflux extracting the mixture for 90 minutes. After the extraction, the extract is concentrated and dried to obtain the Chinese medicine composition.

[0043] The solvent used is an ethanol aqueous solution with an ethanol volume fraction of 75%.

[0044] Example 3 Preparation of Chinese medicine composition

[0045] The Chinese medicinal raw materials are composed by weight: 30 parts of astragalus; 25 parts of wolfberry; 15 parts of Millettia reticulata; 10 parts of epimedium; 25 parts of saffron; 15 parts of burdock root;

[0046] The preparation method of the Chinese medicine composition comprises: mixing astragalus, wolfberry, epimedium, millettia reticulata, saffron and burdock root, crushing the mixture and passing through a 100-mesh sieve, then heating the mixture to boiling state with a solvent of 12 times the total weight of the Chinese medicine raw materials, and then reflux extracting the mixture for 90 minutes. After the extraction, the extract is concentrated and dried to obtain the Chinese medicine composition.

[0047] The solvent is a mixed solvent consisting of an ethanol aqueous solution, acetone and ethyl acetate in a volume ratio of 5:2:2; the ethanol aqueous solution is an ethanol aqueous solution with an ethanol volume fraction of 75%.

[0048] Comparative Example 1 Preparation of Chinese medicine composition

[0049] The Chinese medicinal raw materials are composed by weight: 30 parts of astragalus; 25 parts of wolfberry; 15 parts of Millettia reticulata; 10 parts of epimedium; 25 parts of saffron;

[0050] The preparation method of the Chinese medicine composition comprises: mixing astragalus, wolfberry, epimedium, millettia reticulata and saffron, crushing the mixture and passing through a 100-mesh sieve, then heating the mixture to boiling state with a solvent of 12 times the total weight of the Chinese medicine raw materials, and then reflux extraction for 90 minutes. After the extraction, the extract is concentrated and dried to obtain the Chinese medicine composition;

[0051] The solvent used is an ethanol aqueous solution with an ethanol volume fraction of 75%.

[0052] The difference between Comparative Example 1 and Example 2 is that in Comparative Example 1, saffron is only added to four Chinese medicinal herbs including astragalus, wolfberry, epimedium and Millettia reticulata; while in Example 2, saffron and burdock root are added at the same time.

[0053] Comparative Example 2 Preparation of Chinese medicine composition

[0054] The Chinese medicinal raw materials are composed by weight: 30 parts of astragalus; 25 parts of wolfberry; 15 parts of Millettia reticulata; 10 parts of epimedium; 15 parts of burdock root;

[0055] The preparation method of the Chinese medicine composition comprises: mixing astragalus, wolfberry, epimedium, millettia spatholobi and burdock root, crushing and passing through a 100-mesh sieve, then heating to boiling state with a solvent of 12 times the total weight of the Chinese medicine raw materials, and then reflux extraction for 90 minutes. After the extraction is completed, the extract is concentrated and dried to obtain the Chinese medicine composition;

[0056] The solvent used is an ethanol aqueous solution with an ethanol volume fraction of 75%.

[0057] The difference between Comparative Example 2 and Example 2 is that in Comparative Example 1, only burdock root is added to four Chinese medicinal herbs including astragalus, wolfberry, epimedium and Millettia reticulata; while in Example 2, saffron and burdock root are added at the same time.

[0058] Comparative Example 3 Preparation of Chinese medicine composition

[0059] The Chinese medicinal raw materials are composed by weight: 30 parts of astragalus; 25 parts of wolfberry; 15 parts of Millettia reticulata; 10 parts of epimedium; 25 parts of saffron; 15 parts of burdock root;

[0060] The preparation method of the Chinese medicine composition comprises: mixing astragalus, wolfberry, epimedium, millettia reticulata, saffron and burdock root, crushing the mixture and passing through a 100-mesh sieve, then heating the mixture to boiling state with a solvent of 12 times the total weight of the Chinese medicine raw materials, and then reflux extracting the mixture for 90 minutes. After the extraction, the extract is concentrated and dried to obtain the Chinese medicine composition.

[0061] The solvent is a mixed solvent consisting of an ethanol aqueous solution and acetone in a volume ratio of 5:2; the ethanol aqueous solution is an ethanol aqueous solution with an ethanol volume fraction of 75%.

[0062] The difference between Comparative Example 3 and Example 3 is that the composition of the organic solvent is different during the preparation of the traditional Chinese medicine composition. Comparative Example 3 uses a mixed solvent consisting of an ethanol aqueous solution and acetone; while Example 3 uses a mixed solvent consisting of an ethanol aqueous solution, acetone and ethyl acetate.

[0063] Comparative Example 4 Preparation of Chinese medicine composition

[0064] The Chinese medicinal raw materials are composed by weight: 30 parts of astragalus; 25 parts of wolfberry; 15 parts of Millettia reticulata; 10 parts of epimedium; 25 parts of saffron; 15 parts of burdock root;

[0065] The preparation method of the Chinese medicine composition comprises: mixing astragalus, wolfberry, epimedium, millettia reticulata, saffron and burdock root, crushing the mixture and passing through a 100-mesh sieve, then heating the mixture to boiling state with a solvent of 12 times the total weight of the Chinese medicine raw materials, and then reflux extracting the mixture for 90 minutes. After the extraction, the extract is concentrated and dried to obtain the Chinese medicine composition.

[0066] The solvent is a mixed solvent consisting of an ethanol aqueous solution and ethyl acetate in a volume ratio of 5:2; the ethanol aqueous solution is an ethanol aqueous solution with an ethanol volume fraction of 75%.

[0067] The difference between Comparative Example 4 and Example 3 is that the composition of the organic solvent is different during the preparation of the traditional Chinese medicine composition. Comparative Example 3 uses a mixed solvent consisting of an ethanol aqueous solution and ethyl acetate; while Example 3 uses a mixed solvent consisting of an ethanol aqueous solution, acetone and ethyl acetate.

[0068] Comparative Example 5 Preparation of Chinese medicine composition

[0069] The Chinese medicinal raw materials are composed by weight: 30 parts of astragalus; 25 parts of wolfberry; 15 parts of Millettia reticulata; 10 parts of epimedium; 25 parts of saffron; 15 parts of burdock root;

[0070] The preparation method of the Chinese medicine composition comprises: mixing astragalus, wolfberry, epimedium, millettia reticulata, saffron and burdock root, crushing the mixture and passing through a 100-mesh sieve, then heating the mixture to boiling state with a solvent of 12 times the total weight of the Chinese medicine raw materials, and then reflux extracting the mixture for 90 minutes. After the extraction, the extract is concentrated and dried to obtain the Chinese medicine composition.

[0071] The solvent is a mixed solvent consisting of an ethanol aqueous solution, acetonitrile and n-butanol in a volume ratio of 5:2:2; the ethanol aqueous solution is an ethanol aqueous solution with an ethanol volume fraction of 75%.

[0072] The difference between Comparative Example 5 and Example 3 is that the composition of the organic solvent is different during the preparation of the traditional Chinese medicine composition. Comparative Example 5 uses a mixed solvent consisting of an ethanol aqueous solution, acetonitrile and n-butanol; while Example 3 uses a mixed solvent consisting of an ethanol aqueous solution, acetone and ethyl acetate.

[0073] Experimental Example 1

[0074] The NCG-X mice in the following examples were obtained from Jicui Pharmaceutical Co., Ltd. and were used for screening of drugs for treating Mediterranean diseases.

[0075] After feeding for 4 weeks, NCG-X mice were randomly divided into a control group and experimental groups 1 to 8, with 12 mice in each group; the experimental group was gavaged with a Chinese medicine composition suspended in normal saline every day (the gavage dose of the Chinese medicine composition was 50 mg / kg / d), and the control group was gavaged with an equal amount of normal saline every day; after continuous gavage and feeding for 4 weeks, blood was collected from each group of mice, and the number of red blood cells and hemoglobin content in the blood of each group of mice were tested using a blood cell analyzer; the test results are shown in Table 1.

[0076] The experimental groups 1 to 8 were intragastrically administered with the Chinese medicine compositions prepared in Examples 1 to 3 and Comparative Examples 1 to 5 every day.

[0077] Table 1. Experimental results of the Chinese medicine composition of the present invention in treating anemia

[0078]

[0079] It can be seen from the experimental results in Table 1 that the number of red blood cells and the hemoglobin content in the blood of mice in the experimental group 1 are significantly higher than those in the control group; this shows that the Chinese medicine composition prepared by using the Chinese medicines Astragalus, Lycium barbarum, Epimedium and Millettia reticulata as raw materials can effectively increase the number of red blood cells and / or the hemoglobin content in the blood of thalassemia model mice; it can be seen that the Chinese medicine composition of the present invention can play a role in treating anemia by increasing the number of red blood cells and / or the hemoglobin content in the blood, especially playing a role in treating thalassemia.

[0080] It can be seen from the experimental results in Table 1 that the number of red blood cells and the hemoglobin content in the blood of mice in the experimental group 2 are further significantly higher than those in the experimental group 1; this indicates that: in addition to the four Chinese medicinal materials of astragalus, wolfberry, epimedium and Millettia reticulata, saffron and burdock root are further added; the Chinese medicine composition prepared with the six Chinese medicinal materials of astragalus, wolfberry, epimedium, Millettia reticulata, saffron and burdock root as raw materials has an effect of increasing the number of red blood cells and / or hemoglobin content in the blood that is significantly higher than the Chinese medicine composition prepared with the four Chinese medicinal materials of astragalus, wolfberry, epimedium and Millettia reticulata as raw materials; the Chinese medicine composition can have a better effect in treating anemia or thalassemia.

[0081] It can also be seen from the experimental results in Table 1 that the number of red blood cells and the hemoglobin content in the blood of mice in experimental groups 4 and 5 are compared with those in experimental group 1, but the increase is not significant; this shows that: in the four Chinese medicinal materials of astragalus, wolfberry, epimedium and Millettia reticulata, saffron and burdock root must be further added at the same time; the Chinese medicinal composition prepared with six Chinese medicinal materials such as astragalus, wolfberry, epimedium, Millettia reticulata, saffron and burdock root as raw materials must be used at the same time to significantly improve the effect of astragalus, wolfberry, epimedium, Millettia reticulata, saffron and burdock root. The Chinese medicine composition prepared by taking four Chinese medicinal herbs, namely, astragalus, wolfberry, epimedium and Millettia spatholobi as raw materials, has the effect of increasing the number of red blood cells and / or the hemoglobin content in the blood; however, in the Chinese medicine composition prepared by only adding saffron or burdock root to the four Chinese medicinal herbs, namely, astragalus, wolfberry, epimedium and Millettia spatholobi, it cannot significantly improve the effect of the Chinese medicine composition prepared by taking the four Chinese medicinal herbs, namely, astragalus, wolfberry, epimedium and Millettia spatholobi as raw materials, on increasing the number of red blood cells and / or the hemoglobin content in the blood.

[0082] It can be seen from the experimental results in Table 1 that the number of red blood cells and the hemoglobin content in the blood of mice in the experimental group 3 are further significantly higher than those in the experimental group 2; the number of red blood cells and the hemoglobin content in the blood of mice in the experimental groups 6 to 8 are improved compared with those in the experimental group 2, but the increase is not large, and the increase is much smaller than that in the experimental group 3; this shows that the Chinese medicine composition prepared by using different extraction solvents in the preparation process of the Chinese medicine composition of the present invention has different effects on increasing the number of red blood cells and / or hemoglobin content in the blood. The above experimental results show that the Chinese medicine composition prepared by using six Chinese medicines such as astragalus, wolfberry, epimedium, Millettia reticulata, saffron and burdock root as raw materials and using a mixed solvent composed of ethanol aqueous solution, acetone and ethyl acetate has a significantly higher effect on increasing the number of red blood cells and / or hemoglobin content in the blood than the Chinese medicine composition prepared by using conventional ethanol aqueous solution. Moreover, only the Chinese medicine composition prepared by using a mixed solvent consisting of an ethanol aqueous solution, acetone and ethyl acetate has a significantly higher effect on increasing the number of red blood cells and / or the hemoglobin content in the blood than the Chinese medicine composition prepared by using a conventional ethanol aqueous solution; however, the Chinese medicine composition prepared by using a combination of other solvents cannot have a significantly higher effect on increasing the number of red blood cells and / or the hemoglobin content in the blood than the Chinese medicine composition prepared by using a conventional ethanol aqueous solution.

Claims

1. A Chinese medicine composition, characterized in that: The Chinese medicine composition is made from the following raw material components: Astragalus, wolfberry, epimedium, Millettia reticulata, saffron, and burdock root; The weight proportions of astragalus, wolfberry, epimedium, millettia reticulata, saffron and burdock root in the Chinese medicine composition are respectively: 25-40 parts of Astragalus; 20-30 parts of Lycium barbarum; 10-20 parts of Millettia spatholobi; 8-15 parts of Epimedium; 20-30 parts of Saffron; 10-20 parts of Burdock Root.

2. The Chinese medicine composition according to claim 1, characterized in that: The weight proportions of astragalus, wolfberry, epimedium, millettia reticulata, saffron and burdock root in the Chinese medicine composition are respectively: 30 parts of Astragalus; 25 parts of Lycium barbarum; 15 parts of Millettia spatholobi; 10 parts of Epimedium; 25 parts of Saffron; 15 parts of Burdock Root.

3. The method for preparing the Chinese medicine composition according to claim 1 or 2, characterized in that: The following steps are included: The raw material components are mixed and heated to boiling state with a solvent, and then reflux extraction is performed for 30 to 120 minutes. After the extraction is completed, the extract is concentrated and dried to obtain the traditional Chinese medicine composition; The solvent used is a mixed solvent consisting of ethanol aqueous solution, acetone and ethyl acetate; the volume ratio of the ethanol aqueous solution, acetone and ethyl acetate in the mixed solvent is 3-6:1-3:1-3.

4. Use of the Chinese medicine composition according to claim 1 or 2 in the preparation of a medicine for treating thalassemia.

Images