A compound camellia pill for soothing the liver and strengthening the spleen and its preparation method and application
By preparing Shugan Jianpi Compound Chacha Pills, using the combination of Chinese medicinal materials such as camellia, the problems of poor efficacy of existing products and the risk of hormone addition are solved, effective relief and prevention of liver and spleen damage are achieved, and a safety is provided. New effective ideas.
Patent Information
- Application Number
- CN202410893146.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2024-07-04
- Publication Date
- 2024-12-27
- Estimated Expiration
- 2044-07-04
AI Technical Summary
The existing liver-relieving and spleen-enhancing health care products are relatively single, with poor efficacy, and most of the added hormones may damage the body, lacking effective products that have both effects.
Prepare a compound camellia pill with a combination of supplemental liver and healthy spleen, including camellia, gardenia leaves, beef tongue, polygonatum leaves, orange peel, pig gallbladder powder and licorice. The pill is made by pulverizing, condensing and reflux extraction, filtration and mixing auxiliary materials. To relieve liver and spleen damage.
This compound Shancha Pill significantly improves liver and spleen damage in mice, has no toxic side effects, and provides a novel method to prevent and treat liver and spleen damage.
Smart Images
Figure CN118634285B_ABST
Abstract
Description
Technical Field
[0001] The invention relates to the technical field of traditional Chinese medicines, and in particular to a liver-soothing and spleen-strengthening compound camellia pill and a preparation method and application thereof. Background Art
[0002] The liver is an important organ that undertakes various functions such as metabolic regulation and storage of sugar, protein and lipid as the three major nutrients, as well as decomposition and detoxification of substances not needed by the body. Excessive alcohol intake, viral infection, unbalanced eating habits, stress and smoking can cause acute or chronic damage to these functions, and when the damage develops, diseases such as acute hepatitis, chronic hepatitis, cirrhosis, alcoholic fatty liver, hepatitis B virus, liver cancer, etc. will occur. Liver diseases will affect the spleen, leading to abnormal spleen function. "Golden Chamber" says, "When you see a liver disease, you know that the liver will pass it on to the spleen, so you should first strengthen the spleen." The liver belongs to wood, and the spleen belongs to earth. The liver stores blood and is responsible for dredging, while the spleen regulates blood and is responsible for transportation and transformation. It is the source of qi and blood biochemical transformation and the pivot of qi movement. The two coordinate and promote each other physiologically, according to the law of the five elements, that is, earth needs wood to dredge, and wood depends on earth to flourish. The liver and wood disperse the earth, and the earth is able to reach it with the help of wood. If the liver's function of dispersing and dispersing is normal and the qi is smooth, it can promote the spleen and stomach's reception and transportation, so that the spleen's rise and stomach's descent are coordinated and balanced, so that the spleen can move vigorously, biochemically active, and control with power. "Blood Symptoms" says: "The nature of wood is to disperse and disperse. When food qi enters the stomach, it depends entirely on the liver and wood's qi to disperse and disperse it, and then the water and grain are transformed." The earth is the mother of all things and the foundation of the granary. The liver and wood must be nourished by the water and grain essence produced by the spleen and earth, so that it can be smooth and unobstructed, and it will not be too strong, so that the liver and wood can function normally. It can be seen that the liver and spleen affect each other in physiology and pathology. When one side is too much or too little, it breaks this balance, that is, pathological changes occur, and they affect each other and show a series of diseases and symptoms of the liver and spleen.
[0003] At present, there are a variety of health care products on the market that can soothe the liver and strengthen the spleen, but there are few products that have both effects. In addition, even if there are, the effect of the product is single, the curative effect is poor, and even hormones and other ingredients that may damage the body are added. For this reason, the present invention specifically proposes a compound tea pill for soothing the liver and strengthening the spleen to protect the liver and spleen. Summary of the invention
[0004] The purpose of the present invention is to provide a compound camellia pill for soothing the liver and strengthening the spleen and a preparation method and application thereof to solve the problems existing in the above-mentioned prior art. The compound camellia pill for soothing the liver and strengthening the spleen prepared by the present invention can effectively alleviate liver and spleen damage in mice, has no toxic side effects, and can provide a new idea for preventing and treating liver and spleen damage.
[0005] To achieve the above object, the present invention provides the following solutions:
[0006] The invention provides a compound camellia pill for soothing the liver and strengthening the spleen. The compound camellia pill comprises the following raw materials in parts by weight: 10-15 parts of camellia, 6-10 parts of gardenia leaves, 6-10 parts of oxalis, 3-6 parts of polygonatum leaves, 3-6 parts of tangerine peel, 1-3 parts of pig gall powder and 1-3 parts of liquorice.
[0007] The present invention also provides a method for preparing the above-mentioned compound camellia pills for soothing the liver and strengthening the spleen, comprising the following steps:
[0008] Mix camellia, gardenia leaves, oxalis, polygonatum leaves, orange peel and liquorice, and grind them into ultrafine powder; condense and reflux to obtain a filtrate, filter, and take the supernatant and concentrate it into a paste to obtain an extract;
[0009] Add pig bile powder to the extract and stir evenly to obtain a mixed extract;
[0010] The mixed extract is mixed with auxiliary materials to prepare pills.
[0011] Furthermore, the auxiliary materials include one or more of diluents, fillers, excipients, binders, wetting agents, disintegrants, absorption promoters, surfactants, adsorption carriers, lubricants and flavoring agents.
[0012] Furthermore, the auxiliary materials include 0.8 parts of soluble starch, 0.1 parts of camellia oil and 0.04 parts of sucrose.
[0013] Furthermore, the condensation reflux time is 60 minutes.
[0014] Furthermore, the filtration is vacuum negative pressure filtration, and the filtration times are 2 times.
[0015] The present invention also provides an application of the above-mentioned compound camellia pills for soothing the liver and strengthening the spleen in preparing medicines for preventing and treating liver and spleen injuries.
[0016] The present invention also provides a medicine for preventing and treating liver and spleen damage, comprising the above-mentioned compound camellia pills for soothing the liver and strengthening the spleen, and auxiliary materials.
[0017] Furthermore, the dosage form of the drug includes pills.
[0018] Furthermore, the method of taking the drug includes oral and parenteral administration.
[0019] In the compound camellia pill for soothing the liver and strengthening the spleen of the present invention, camellia, gardenia leaves and herba cyperi are used as the main medicines for clearing away the evil of liver and spleen stagnation, polygonatum sibiricum leaves and tangerine peel are used as the assistant medicines for nourishing yin and qi and strengthening the yin of liver and spleen, pig bile powder is used as the adjuvant medicine for clearing away heat and detoxifying, and liquorice is used as the guiding medicine for harmonizing various medicines.
[0020] The present invention discloses the following technical effects:
[0021] The compound Shancha Pill for soothing the liver and strengthening the spleen provided by the present invention comprises camellia, gardenia leaf, ox tongue grass, polygonatum leaf, tangerine peel, pig gall powder and licorice. Through reasonable compatibility, the whole prescription uses camellia, gardenia leaf and ox tongue grass as monarch medicine, polygonatum leaf and tangerine peel as minister medicine, pig gall powder and licorice as assistant medicine, which conforms to the compatibility treatment principle of "monarch, minister, assistant and assistant", and exerts the effect of soothing the liver and strengthening the spleen from the two aspects of "strengthening the body and eliminating evil". It has been verified by experiments that the compound Shancha Pill for soothing the liver and strengthening the spleen prepared by the present invention can significantly improve the liver and spleen damage of mice, has no toxic side effects, and can provide a new idea for preventing and treating liver and spleen damage. BRIEF DESCRIPTION OF THE DRAWINGS
[0022] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.
[0023] Figure 1 Statistical graph of serum AST levels of mice in each group; * indicates P < 0.05, the difference is statistically significant;
[0024] Figure 2 Statistical graph of serum ALT levels of mice in each group; * indicates P < 0.05, the difference is statistically significant;
[0025] Figure 3 The results of HE staining of the liver of mice in each group; A: blank group, B: liver injury group, C: Example 1 group, D: Example 2 group, E: Example 3 group;
[0026] Figure 4 The HE staining results of spleen of mice in each group are shown; A: blank group, B: spleen injury group, C: Example 1 group, D: Example 2 group, E: Example 3 group;
[0027] Figure 5 The statistical graphs of IgG (A) and IgM (B) levels in the serum of mice in each group; * indicates P < 0.05, and the difference is statistically significant. DETAILED DESCRIPTION
[0028] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as limiting the present invention, but should be understood as a more detailed description of certain aspects, features, and embodiments of the present invention.
[0029] It should be understood that the terms described in the present invention are only for describing a particular embodiment and are not intended to limit the present invention. In addition, for the numerical range in the present invention, it should be understood that each intermediate value between the upper and lower limits of the scope is also specifically disclosed. The intermediate value in any stated value or stated range, and each smaller range between any other stated value or intermediate value in the described range is also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded in the scope.
[0030] Unless otherwise indicated, all technical and scientific terms used herein have the same meanings as those generally understood by those skilled in the art. Although the present invention describes only preferred methods and materials, any methods and materials similar or equivalent to those described herein may also be used in the implementation or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials associated with the documents. In the event of a conflict with any incorporated document, the content of this specification shall prevail.
[0031] It will be apparent to those skilled in the art that various modifications and variations may be made to the specific embodiments of the present invention description without departing from the scope or spirit of the present invention. Other embodiments derived from the present invention description will be apparent to those skilled in the art. The present invention description and examples are exemplary only.
[0032] The words “include,” “including,” “have,” “contain,” etc. used in this document are open-ended terms, meaning including but not limited to.
[0033] The experimental methods in the following examples, unless otherwise specified, are all conventional methods, and are performed according to the techniques or conditions described in the literature in the field or according to the product instructions. The materials, reagents, etc. used in the following examples, unless otherwise specified, can all be obtained from commercial channels.
[0034] Female ICR mice were purchased from Henan Sikebes Biotechnology Co., Ltd., weighing 18±2g. The temperature of all experimental animals was maintained between 22-26°C, and the relative humidity was between 40%-70%. All mice were kept in SPF cages with a day and night cycle of 12h light and 12h dark (8:00 to 20:00 every day). Each experimental animal was allowed to feed standard mouse food and water at will. The experiment was carried out again after one week of adaptive feeding. The specific research of the present invention is as follows:
[0035] Example 1
[0036] A compound camellia pill for soothing the liver and strengthening the spleen comprises the following raw materials in parts by weight: 12 parts of camellia, 8 parts of gardenia leaves, 9 parts of oxalis, 4 parts of polygonatum leaves, 3 parts of tangerine peel, 1 part of pig gall powder and 1 part of liquorice.
[0037] Preparation method: Mix camellia, gardenia leaves, oxalis, polygonatum leaves, orange peel and licorice, and crush them into ultrafine powder by a wall-breaking machine; add 15 times the volume of water to the ultrafine powder, condense and reflux for 60 minutes to obtain an extract, vacuum negative pressure filter, collect the filtrate, filter again to obtain the supernatant, concentrate into a paste, and obtain an extract; add pig gall powder to the extract and stir evenly to obtain a mixed extract, take 1 part of the mixed extract and auxiliary materials (0.8 parts of soluble starch, 0.1 parts of camellia oil and 0.04 parts of sucrose) by weight, mix evenly, make pills, and seal and store. So as to obtain the compound camellia pills for soothing the liver and strengthening the spleen.
[0038] Example 2
[0039] A compound camellia pill for soothing the liver and strengthening the spleen comprises the following raw materials in parts by weight: 10 parts of camellia, 9 parts of gardenia leaves, 6 parts of oxalis, 6 parts of polygonatum leaves, 4 parts of tangerine peel, 2 parts of pig gall powder and 3 parts of liquorice.
[0040] The preparation method is the same as that of Example 1.
[0041] Example 3
[0042] A compound camellia pill for soothing the liver and strengthening the spleen comprises the following raw materials in parts by weight: 15 parts of camellia, 6 parts of gardenia leaves, 8 parts of ragwort, 3 parts of polygonatum leaves, 6 parts of tangerine peel, 3 parts of pig gall powder and 2 parts of liquorice.
[0043] The preparation method is the same as that of Example 1.
[0044] Effect example
[0045] 1. Therapeutic effect of Soothing Liver and Strengthening Spleen Compound Shancha Pill on mice with liver injury
[0046] 25 healthy female ICR mice were randomly divided into five groups: blank group, liver injury group, Example 1 group, Example 2 group, and Example 3 group. The liver injury group, Example 1 group, Example 2 group, and Example 3 group were intraperitoneally injected with carbon tetrachloride (2%) at a dose of 0.01 mL / g every day for 3 consecutive days to establish a liver injury model. After the model was established, Example 1 group, Example 2 group, and Example 3 group were treated with oral gavage (0.5 g / kg body weight dose) of the Soothing Liver and Strengthening Spleen Compound Camellia Pills prepared in Example 1, Example 2, and Example 3, respectively, for 3 consecutive days. After the treatment, the mice were killed, blood was collected for serum separation, and the liver was collected and stored in 4% paraformaldehyde. The following experiments were performed.
[0047] 1.1 Mouse liver function test
[0048] The liver function of mice was detected according to the instructions of the aspartate aminotransferase (AST) and alanine aminotransferase (ALT) kits provided by Nanjing Jiancheng Biological Research Institute.
[0049] The test results are as follows Figure 1-2 As shown, compared with the blank group, the serum AST and ALT levels of mice in the liver injury group were significantly increased (P<0.05), and compared with the liver injury group, the serum AST and ALT levels of mice in Example 1, Example 2, and Example 3 were significantly reduced (P<0.05). This shows that the liver injury model was successfully established by the present invention, and the compound Shancha pills for soothing the liver and strengthening the spleen prepared in Examples 1-3 of the present invention can effectively improve the liver injury of mice.
[0050] 1.2 Liver pathological tissue sections
[0051] The liver tissue preserved in 4% paraformaldehyde solution was removed and cut into 0.5 mm 3 The tissue blocks were embedded in paraffin using conventional tissue embedding techniques, sliced and dewaxed using a tissue slicer, and then prepared according to the HE staining method. They were photographed under a digital camera microscope to observe the pathological histological changes of the liver.
[0052] HE staining results Figure 3 As shown, compared with the blank group, a large number of necrosis occurred in the liver cells of the mice in the liver injury group, and the liver cells were arranged in disorder. Compared with the liver injury group, the liver necrotic cells in the Example 1 group, the Example 2 group, and the Example 3 group were significantly reduced, and the liver cells were arranged normally. The test results also show that the compound Shancha pills for soothing the liver and strengthening the spleen prepared in Examples 1-3 of the present invention can effectively improve the liver injury of mice.
[0053] 2. The therapeutic effect of Shancha Pills for soothing the liver and strengthening the spleen on mice with spleen injury
[0054] Take 25 healthy female ICR mice and randomly divide them into five groups: blank group, spleen injury group, Example 1 group, Example 2 group, and Example 3 group. Example 1 group, Example 2 group, and Example 3 group were gavaged with the compound tea pills for soothing the liver and strengthening the spleen prepared in Example 1, Example 2, and Example 3 (0.1 g / kg body weight dose) for 7 consecutive days. On the 5th to 7th day of gavage, the spleen injury group, Example 1 group, Example 2 group, and Example 3 group were intraperitoneally injected with cyclophosphamide at a dose of 5 mg / kg body weight every day for 3 consecutive days to establish a spleen injury model. After the treatment, the mice were killed, blood was collected for serum separation, and the spleen was collected and stored in 4% paraformaldehyde. The following experiments were performed.
[0055] 2.1 Spleen pathological tissue sections
[0056] The spleen tissue preserved in 4% paraformaldehyde solution was removed and cut into 0.5 mm 3 The tissue blocks were embedded in paraffin using conventional tissue embedding techniques, sliced and dewaxed using a tissue slicer, and then prepared according to the HE staining method. They were photographed under a digital camera microscope to observe the pathological histological changes of the spleen.
[0057] HE staining results Figure 4 As shown, the results show that the red pulp and white pulp of the blank group have clear structures, obvious boundaries, moderate thickness, and no obvious lesions are found; the red pulp and white pulp boundaries of the spleen injury group are blurred, the lymphocytes are sparsely arranged and edematous, and local lesions are obvious; the boundaries of the white pulp and red pulp of Example 1, Example 2, and Example 3 groups are clear, the splenic cords and splenic sinuses have regular shapes, the lymphocyte edema is reduced, and the local lesions are significantly improved. This shows that the Soothing Liver and Strengthening Spleen Compound Shancha Pills prepared in Examples 1-3 of the present invention can effectively improve the spleen injury of mice.
[0058] 2.2 Determination of serum immune factor levels
[0059] The IgG and IgM levels in mouse serum were detected according to the instructions of Wuhan Elabscience Mouse IgG and IgM ELISA.
[0060] Test results such as Figure 5 As shown, compared with the blank group, the IgG and IgM levels in the serum of mice in the spleen injury group were significantly reduced (P<0.05), and compared with the spleen injury group, the IgG and IgM levels in the serum of Example 1, Example 2, and Example 3 groups were significantly increased (P<0.05) and had no significant difference compared with the blank group. This shows that the immune ability of mice in the spleen injury group decreased, and the liver-soothing and spleen-strengthening compound Shancha pills prepared in Examples 1-3 of the present invention can significantly enhance the immune ability of mice and play a role in protecting spleen function.
[0061] In summary, the compound Shancha pills for soothing the liver and strengthening the spleen prepared by the present invention can significantly improve the liver and spleen damage in mice, have no toxic side effects, and can provide a new idea for preventing and treating liver and spleen damage.
[0062] The embodiments described above are only descriptions of the preferred modes of the present invention, and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by ordinary technicians in this field should all fall within the protection scope determined by the claims of the present invention.
Claims
1. A compound camellia pill for soothing the liver and strengthening the spleen, characterized in that: The invention comprises the following raw materials in parts by weight: 10-15 parts of camellia, 6-10 parts of gardenia leaves, 6-10 parts of oxalis, 3-6 parts of polygonatum leaves, 3-6 parts of orange peel, 1-3 parts of pig gall powder and 1-3 parts of liquorice.
2. A method for preparing the compound camellia pills for soothing the liver and strengthening the spleen as claimed in claim 1, characterized in that: The following steps are involved: Mix camellia, gardenia leaves, oxalis, polygonatum leaves, orange peel and liquorice, and grind them into ultrafine powder; condense and reflux to obtain an extract, filter, and take the supernatant and concentrate it into a paste to obtain an extract; Add pig bile powder to the extract and stir evenly to obtain a mixed extract; The mixed extract is mixed with auxiliary materials to prepare pills.
3. The preparation method of compound Shancha pills for soothing the liver and strengthening the spleen according to claim 2, characterized in that: The auxiliary materials include one or more of diluents, fillers, excipients, binders, wetting agents, disintegrants, absorption promoters, surfactants, adsorption carriers, lubricants and flavoring agents.
4. The preparation method of compound Shancha pills for soothing the liver and strengthening the spleen according to claim 2, characterized in that: In parts by weight, the auxiliary materials include 0.8 parts of soluble starch, 0.1 parts of camellia oil and 0.04 parts of sucrose.
5. The preparation method of compound Shancha pills for soothing the liver and strengthening the spleen according to claim 2, characterized in that: The condensation reflux time is 60 min.
6. The method for preparing the compound Shancha pills for soothing the liver and strengthening the spleen according to claim 2, characterized in that: The filtration was vacuum negative pressure filtration, and the filtration times were 2 times.
7. Use of the compound Shancha pills for soothing the liver and strengthening the spleen according to claim 1 in preparing medicines for preventing and treating liver and spleen damage.
8. A drug for preventing and treating liver and spleen damage, characterized in that: It comprises the compound camellia pill for soothing the liver and strengthening the spleen as described in claim 1, and auxiliary materials.
9. The drug according to claim 8, characterized in that The dosage form of the drug includes pills.
10. The drug according to claim 8, characterized in that The method of taking the drug includes oral administration.