Spectral signal detection system, method and related products

Abstract

The present application provides a spectral signal detection system, method and related products. The spectral signal detection system includes: a light source, an optical fiber path, and a spectral detector; the optical fiber path includes a light input section, a connecting part, a biological detection sensor and a light output section, the biological detection sensor includes a light input section, a first sensing part, a second sensing part, a light output section and a light transmission part, one end of the light input section is connected to one end of the light input section through the connecting part, the light source is located at the other end of the light input section, one end of the light output section is connected to one end of the light output section through the connecting part, and the spectral detector is connected to the other end of the light output section; the first sensing part is connected to the other end of the light input section and to one end of the light transmission section, the second sensing part is connected to the other end of the light output section and to the other end of the light transmission section. By setting the shape of the biological detection sensor to be "U"-shaped and symmetrically setting two sensing parts, the interference of background liquid can be eliminated and the accuracy of detection can be improved.

Description

Technical Field

[0001] The present application relates to the field of biological detection technology, and in particular to a spectral signal detection system, method and related products. Background Art

[0002] Optical surface plasmon resonance (SPR) detection technology is a new biochemical analysis method that is extremely sensitive to tiny changes in refractive index. Any biochemical reaction will inevitably cause tiny changes in the refractive index of the detection environment. Therefore, SPR technology can be widely used in various biochemical detection fields.

[0003] At present, when a single SPR sensor is performing detection, it is often interfered by the temperature of the background liquid, the molecules in the background liquid, the concentration of the background liquid, etc., resulting in inaccurate detection results. Summary of the invention

[0004] In view of this, the present application provides a spectral signal detection system and related products to improve the accuracy of detection.

[0005] In a first aspect, the present application provides a spectral signal detection system, the spectral signal detection system comprising:

[0006] light source;

[0007] An optical fiber pathway, the optical fiber pathway comprising a light input section, a connecting portion, a biological detection sensor and a light output section, the biological detection sensor comprising a light input section, a first sensing portion, a second sensing portion, a light output portion and a light transmission portion; one end of the light input section is connected to one end of the light input section through the connecting portion, the light source is located at the other end of the light input section, one end of the light output section is connected to one end of the light output section through the connecting portion, and the spectrum detector is connected to the other end of the light output section; two ends of the first sensing portion are respectively connected to the other end of the light input section and one end of the light transmission portion, and two ends of the second sensing portion are respectively connected to the other end of the light output section and the other end of the light transmission portion;

[0008] A spectral detector, the spectral detector is used to collect a target spectral signal of the output light from the biological detection sensor, determine a first resonance peak wavelength and a second resonance peak wavelength of the target spectral signal, determine a target change quantity characterization value according to the first resonance peak wavelength and the second resonance peak wavelength, and determine a target concentration of the target molecule according to the target change quantity characterization value and a set of change quantity characterization value mapping relationships; the target spectral signal is a detection signal of the target molecule in the detection solution adsorbed on the second metal film surface of the second sensing part in a steady state and under other condition constraints, and the target change quantity characterization value is used to characterize the change in the absorption capacity of the biological detection sensor for the incident light of the light source under the constraint of steady state when the target molecule is adsorbed on the second metal film surface.

[0009] In a possible example, the light transmission portion is bent, the first sensing portion and the second sensing portion are symmetrically arranged, and the light incident portion and the light emitting portion are symmetrically arranged.

[0010] In a possible example, the incident light is emitted after passing through the light incident part, the first sensing part, the light transmission part, the second sensing part and the light output part of the biological detection sensor in sequence, and the incident light has the same first transmission direction in the light incident part and the light transmission part, and the incident light has the same second transmission direction in the light output part and the light transmission part, and the first transmission direction is opposite to the second transmission direction.

[0011] In a possible example, the light incident portion includes a first optical fiber, and the first optical fiber includes a first cladding and a first core;

[0012] The first sensing part includes a second optical fiber and a first metal film wrapped on the surface of the second optical fiber, the second optical fiber is connected to the first fiber core, the incident light enters the second optical fiber from the first fiber core, and generates a plasma resonance reaction on the first metal film;

[0013] The light transmission part includes a third optical fiber, the third optical fiber includes a second cladding and a second core, the second core is connected to an end of the second optical fiber away from the first core, and the incident light enters the second core through the second optical fiber;

[0014] The second sensing part includes a fourth optical fiber and the second metal film wrapped on the surface of the fourth optical fiber, the fourth optical fiber is connected to the end of the second fiber core away from the second optical fiber, the incident light enters the fourth optical fiber from the second fiber core, and generates a plasma resonance reaction on the second metal film;

[0015] The light output portion includes a fifth optical fiber, the fifth optical fiber includes a third cladding and a third core, the third core is connected to an end of the fourth optical fiber away from the second core, the incident light enters the third core through the fourth optical fiber, and is emitted through the third core and enters the spectrum detector.

[0016] In one possible example, the first metal film is modified with a sealing agent layer, the sealing agent layer includes a sealing agent, and the second metal film is modified with a capture functional layer, the capture functional layer includes a target target for capturing the target molecule and the sealing agent, and the sealing agent is used to seal the area on the second metal film that is not modified with the target target.

[0017] In a possible example, the first optical fiber, the third optical fiber, and the fifth optical fiber are multimode optical fibers or single-mode optical fibers;

[0018] The second optical fiber and the fourth optical fiber are multimode optical fibers or single-mode optical fibers or coreless optical fibers or other optical fiber structures that excite the evanescent field on the optical fiber surface.

[0019] In a possible example, the light incident portion includes a first connection area, the first connection area is arranged on a side of the first cladding adjacent to the first sensing portion, and two ends of the first connection area are respectively connected to the second optical fiber and the first cladding;

[0020] The light transmission portion includes a second connection area and a third connection area, the second connection area is arranged on a side of the second cladding adjacent to the first sensing portion, and two ends of the second connection area are respectively connected to the second optical fiber and the second cladding, the third connection area is arranged on a side of the second cladding adjacent to the second sensing portion, and two ends of the third connection area are respectively connected to the fourth optical fiber and the second cladding;

[0021] The light emitting portion includes a fourth connecting region, the fourth connecting region is arranged on a side of the third cladding layer adjacent to the second sensing portion, and two ends of the fourth connecting region are respectively connected to the fourth optical fiber and the third cladding layer.

[0022] In a possible example, the target variation characterization value is a relative difference between the first resonance peak wavelength and the second resonance peak wavelength or a division result value;

[0023] The variation characterization value mapping relationship set includes a corresponding relationship between the variation characterization value and the concentration of the target molecule, and the variation characterization value and the concentration of the target molecule are in a positive correlation relationship.

[0024] In a second aspect, the present application further provides a spectral signal detection method, which is applied to a spectral detector in a spectral signal detection system, wherein the spectral signal detection system includes a light source, an optical fiber path and the spectral detector, wherein the optical fiber path includes a light input section, a connecting portion, a biological detection sensor and a light output section, wherein the biological detection sensor includes a light input section, a light output section, a first sensing portion, a second sensing portion and a light transmission portion, wherein the connecting portion respectively connects the light input section and the light input section, the connecting portion respectively connects the light output section and the light output section, the first sensing portion respectively connects the light input section and the light transmission portion, and the second sensing portion respectively connects the light transmission portion and the light output portion; the method includes:

[0025] Acquire a target spectral signal of the outgoing light from the biological detection sensor, wherein the target spectral signal is a detection signal of the target molecule in the detection solution adsorbed on the surface of the second metal film of the second sensing part in a steady state and under other conditions;

[0026] Determine a first resonance peak wavelength and a second resonance peak wavelength in the target spectral signal;

[0027] Determine a target variation characterization value according to the first resonance peak wavelength and the second resonance peak wavelength, wherein the target variation characterization value is used to characterize a variation in the absorption capacity of the biosensor to the incident light of the light source under steady-state constraints when the target molecule is adsorbed on the surface of the second metal film;

[0028] The target concentration of the target molecule is determined according to the target variation characterization value and the variation characterization value mapping relationship set.

[0029] In a third aspect, the present application also provides a molecular interaction instrument, which is connected to a spectral detector in a spectral signal detection system, and is used to obtain a target change quantity characterization value from the spectral detector, and analyze the strength of the interaction affinity between the target molecule and the target targeting object of the target molecule based on the target change quantity characterization value.

[0030] In a fourth aspect, the present application also provides a portable fiber optic biological detection device, which includes a shell, a display screen and the spectral signal detection system. The spectral signal detection system is accommodated in the shell, and the display screen is arranged outside the shell and is used to display detection information.

[0031] The spectral signal detection system provided in this embodiment changes the direction of light transmission by bending the light transmission part of the biological detection sensor, without setting additional optical components such as reflectors, thereby reducing the manufacturing cost of the biological detection sensor; and, the first sensing part and the second sensing part are symmetrically set on the biological detection sensor, the first sensing part does not modify the target target of the target molecule, and is sealed with a sealing agent, and the second sensing part modifies the target target. Due to the bending setting of the light transmission part, the incident light of the light source can be modulated by the first sensing part and the second sensing part in sequence. The spectral detector collects the target spectral signal of the modulated output light, and determines the first resonance peak wavelength and the second resonance peak wavelength of the target spectral signal. The concentration of the target molecule is analyzed based on the first resonance peak wavelength and the second resonance peak wavelength, eliminating the interference of the background liquid, which is conducive to improving the accuracy of the detection. And, when the spectral signal detection system detects the target solution, the biological detection sensor can be directly immersed in the detection solution without the need for complex sample transfer steps, which is conducive to improving the detection efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0033] Figure 1 It is a structural schematic diagram of a spectral signal detection system provided in an embodiment of the present application;

[0034] Figure 2 is a schematic structural diagram of a biological detection sensor provided in an embodiment of the present application;

[0035] Figure 3 is a structural schematic diagram of another spectral signal detection system provided in an embodiment of the present application;

[0036] Figure 4 is a schematic diagram of a light transmission path provided in an embodiment of the present application;

[0037] Figure 5 is a schematic diagram of another biological detection sensor provided in an embodiment of the present application;

[0038] Figure 6 is a schematic diagram of a spectral signal provided in an embodiment of the present application;

[0039] Figure 7 It is a structural schematic diagram of another spectral signal detection system provided in an embodiment of the present application;

[0040] Figure 8 is a structural schematic diagram of a spectral signal detection system including an interactometer provided in an embodiment of the present application;

[0041] Fig. 9 is a flow chart of a spectral signal detection method provided in an embodiment of the present application;

[0042] Fig.10 It is a schematic diagram of the structure of a portable optical fiber biological detection device according to an embodiment of the present application.

[0043] Description of reference numerals:

[0044] Spectral signal detection system 1, portable optical fiber biological detection device 2, light source 10, optical fiber channel 20, spectral detector 30, light input section 201, connecting part 202, biological detection sensor 203, light output section 204, light input part 2031, first sensing part 2032, second sensing part 2034, light output part 2035, light transmission part 2033, first port 2021, second port 2022, third port 2023, fourth port 2024, first optical fiber 41 , first cladding 401, first fiber core 402, second optical fiber 51, first metal film 52, third optical fiber 61, second cladding 601, second fiber core 602, fourth optical fiber 71, second metal film 72, fifth optical fiber 81, third cladding 801, third fiber core 802, first connection area 91, second connection area 92, third connection area 93, fourth connection area 94, detection channel 100, optical path switch 110, interaction instrument 120, shell 21, display screen 22. DETAILED DESCRIPTION

[0045] In order to enable those skilled in the art to better understand the solution of the present application, the technical solution in the embodiments of the present application will be clearly and completely described below in conjunction with the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of this application.

[0046] The terms "first", "second", etc. in the specification and claims of this application and the above-mentioned drawings are used to distinguish different objects, rather than to describe a specific order. In addition, the terms "including" and "having" and any variations thereof are intended to cover non-exclusive inclusions. For example, a process, method, system, product or device that includes a series of steps or units is not limited to the listed steps or units, but optionally includes steps or units that are not listed, or optionally includes other steps or units inherent to these processes, methods, products or devices.

[0047] Reference to "embodiments" herein means that a particular feature, structure, or characteristic described in conjunction with the embodiments may be included in at least one embodiment of the present application. The appearance of the phrase in various locations in the specification does not necessarily refer to the same embodiment, nor is it an independent or alternative embodiment that is mutually exclusive with other embodiments. It is explicitly and implicitly understood by those skilled in the art that the embodiments described herein may be combined with other embodiments.

[0048] In the embodiments of the present application, "and / or" describes the association relationship of the associated objects, indicating that three relationships may exist. For example, A and / or B can represent the following three situations: A exists alone; A and B exist at the same time; B exists alone. Among them, A and B can be singular or plural.

[0049] In the embodiment of the present application, the symbol " / " can indicate that the objects associated with each other are in an "or" relationship. In addition, the symbol " / " can also indicate a division sign, that is, performing a division operation. For example, A / B can indicate A divided by B.

[0050] In the embodiments of the present application, "at least one item" or similar expressions refer to any combination of these items, including any combination of single items or plural items, and refer to one or more, and multiple refers to two or more. For example, at least one item of a, b, or c can represent the following seven situations: a, b, c, a and b, a and c, b and c, a, b, and c. Among them, each of a, b, and c can be an element or a set containing one or more elements.

[0051] In the embodiments of the present application, "equal to" can be used in conjunction with greater than, and is applicable to the technical solution adopted when greater than, and can also be used in conjunction with less than, and is applicable to the technical solution adopted when less than. When equal to is used in conjunction with greater than, it is not used in conjunction with less than; when equal to is used in conjunction with less than, it is not used in conjunction with greater than.

[0052] In order to better understand the solutions of the embodiments of the present application, the electronic devices, related concepts and backgrounds that may be involved in the embodiments of the present application are first introduced below.

[0053] The electronic devices involved in the embodiments of the present application may include various handheld devices with wireless communication functions, vehicle-mounted devices, wearable devices, computing devices or other processing devices connected to a wireless modem, as well as various forms of user equipment (UE), mobile stations (MS), electronic devices (terminal devices), etc. For the convenience of description, the above-mentioned devices are collectively referred to as electronic devices. The electronic device can also be a spectral detector in an optical fiber biological detection system.

[0054] See also Figure 1 , Figure 2 and Figure 3 , Figure 1 is a structural schematic diagram of a spectral signal detection system provided in an embodiment of the present application, Figure 2 is a schematic diagram of the structure of a biological detection sensor provided in an embodiment of the present application, Figure 3 1 is a schematic diagram of the structure of another spectral signal detection system provided in an embodiment of the present application. The spectral signal detection system 1 of this embodiment includes a light source 10, an optical fiber path 20 and a spectral detector 30, the optical fiber path 20 includes a light input section 201, a connecting part 202, a biological detection sensor 203 and a light output section 204, and the biological detection sensor 203 includes a light input section 2031, a first sensing section 2032, a second sensing section 2034, a light output section 2035 and a light transmission section 2033.

[0055] Among them, the light source 10 is used to emit light, and the emitted light is broadband white light; one end of the light input section 201 is connected to one end of the light input section 2031 through the connecting part 202, the light source 10 is located at the other end of the light input section 201, one end of the light output section 204 is connected to one end of the light output section 2035 through the connecting part 202, and the spectrum detector 30 is connected to the other end of the light output section 204; the first sensing part 2032 is connected to the other end of the light input section 2031, and is connected to one end of the light transmission part 2033, and the second sensing part 2034 is connected to the other end of the light output section 2035, and is connected to the other end of the light transmission part 2033.

[0056] Among them, broadband white light can be understood as a composite light mixed from multiple monochromatic lights, and its spectral range covers most or all of the visible light bands.

[0057] Among them, the connecting part 202 can be a fiber optic connector, and the connecting part 202 includes a first port 2021, a second port 2022, a third port 2023 and a fourth port 2024. The light input section 201 is connected to the first port 2021, the light input section 2031 is connected to the second port 2022, the light output section 2035 is connected to the third port 2023, and the light output section 204 is connected to the fourth port 2024; the first port 2021 is connected to the second port 2022, and the third port 2023 is connected to the fourth port 2024.

[0058] The spectral detector 30 is used to collect the target spectral signal of the outgoing light from the biological detection sensor 203, determine the first resonance peak wavelength and the second resonance peak wavelength of the target spectral signal, determine the target variation characterization value according to the first resonance peak wavelength and the second resonance peak wavelength, and determine the target concentration of the target molecule according to the target variation characterization value and the variation characterization value mapping relationship set; the target spectral signal is the detection signal of the target molecule in the detection solution adsorbed on the surface of the second metal film 72 of the second sensing part 2034 under the constraints of steady state and other conditions, and the target variation characterization value is used to characterize the change in the absorption capacity of the biological detection sensor 203 to the incident light of the light source 10 under the constraints of steady state when the target molecule is adsorbed on the surface of the second metal film 72. Other conditions include temperature conditions, background liquid component conditions, background liquid concentration conditions, and pH conditions. The background liquid component conditions include the components of the background liquid and the interactions between the components. Other conditions will affect the refractive index of the metal film surface, thereby affecting the accuracy of the detection.

[0059] The target molecule may be an antigen, and the target targeting substance may be an antibody, which is not limited here. The target targeting substance may react with the target molecule to achieve stable adsorption of the target molecule by the second sensing portion 2034 .

[0060] It can be understood that in other embodiments of the present application, the antibody can be fixed to the surface of the metal film through a biological cross-linking agent, for example, the biological cross-linking agent is dopamine, carbodiimide, etc.

[0061] Optionally, there is no limitation on the type of antibodies, and by modifying different types of antibodies, the biosensor 203 can become a tool for different specific detections, such as specific detection of proteins, nucleic acids, cells, etc.

[0062] Preferably, the shape of the biometric detection sensor 203 is "U"-shaped or approximate to "U". Specifically, the light transmission portion 2033 is bent, and the bent shape is "U"-shaped or approximate to "U", and the first sensing portion 2032, the second sensing portion 2034, the light input portion 2031 and the light output portion 2035 are all straight.

[0063] Optionally, the first sensing portion 2032 and the second sensing portion 2034 are symmetrically arranged, and are parallel to or approximately parallel to each other; the light incident portion 2031 and the light emitting portion 2035 are symmetrically arranged, and are parallel to or approximately parallel to each other.

[0064] In this embodiment, the propagation paths of the incident light and the outgoing light inside the biological detection sensor 203 do not overlap each other, so there is no need to use a spectroscopic coupler to split the incident light and the outgoing light, making the detection result of the biological detection sensor 203 more accurate and reliable.

[0065] Among them, see Figure 4 , Figure 4 1 is a schematic diagram of a light transmission path provided by an embodiment of the present application. The transmission path of the incident light emitted by the light source 10 is as follows: the incident light enters the light input section 201 in the optical fiber path 20, and enters the light input section 2031 after passing through the connecting section 202, enters the first sensing section 2032 after passing through the light input section 2031, enters the light transmission section 2033 after being modulated by the first sensing section 2032, enters the second sensing section 2034 after passing through the light transmission section 2033, enters the light output section 2035 after being modulated by the second sensing section 2034, enters the light output section 2035 after passing through the light output section 2035, enters the light output section 204 through the connecting section 202, and is emitted through the light output section 204. The incident light has the same first transmission direction in the light input section 2031 and the light transmission section 2033, and has the same second transmission direction in the light output section 2035 and the light transmission section 2033, and the first transmission direction is opposite to the second transmission direction.

[0066] See also Figure 5 , Figure 5 is a schematic diagram of another biological detection sensor provided in an embodiment of the present application, wherein the light input portion 2031 includes a first optical fiber 41, wherein the first optical fiber 41 includes a first cladding 401 and a first core 402, wherein the first sensing portion 2032 includes a second optical fiber 51 and a first metal film 52 wrapped around the surface of the second optical fiber 51, wherein the second optical fiber 51 is connected to the first core 402, wherein the incident light enters the second optical fiber 51 from the first core 402 and generates a plasma resonance reaction on the first metal film 52; wherein the light transmission portion 2033 includes a third optical fiber 61, wherein the third optical fiber 61 includes a second cladding 601 and a second core 602, wherein the second core 602 is connected to an end of the second optical fiber 51 away from the first core 402, wherein the incident light passes through the second core 402 and enters the second optical fiber 51. The optical fiber 51 enters the second fiber core 602; the second sensing part 2034 includes a fourth optical fiber 71 and the second metal film 72 wrapped on the surface of the fourth optical fiber 71, the fourth optical fiber 71 is connected to the end of the second fiber core 602 away from the second optical fiber 51, the incident light enters the fourth optical fiber 71 from the second fiber core 602, and generates a plasma resonance reaction on the second metal film 72; the light output part 2035 includes a fifth optical fiber 81, the fifth optical fiber 81 includes a third cladding 801 and a third fiber core 802, the third fiber core 802 is connected to the end of the fourth optical fiber 71 away from the second fiber core 602, the incident light enters the third fiber core 802 through the fourth optical fiber 71, and is emitted through the third fiber core 802 to enter the spectrum detector 30.

[0067] The light input section 201 includes a sixth optical fiber connected to the first port 2021 of the connecting portion 202 , and the light output section 204 includes a seventh optical fiber connected to the fourth port 2024 of the connecting portion 202 .

[0068] Among them, the first optical fiber 41, the third optical fiber 61, the fifth optical fiber 81, the sixth optical fiber and the seventh optical fiber are multimode optical fibers or single-mode optical fibers; the second optical fiber 51 and the fourth optical fiber 71 are multimode optical fibers or single-mode optical fibers or coreless optical fibers or other optical fiber structures that excite the evanescent field on the optical fiber surface.

[0069] Optionally, the light input portion 2031 includes a first connection area 91, which is arranged on a side of the first cladding 401 adjacent to the first sensing portion 2032, and two ends of the first connection area 91 are respectively connected to the second optical fiber 51 and the first cladding 401; the light transmission portion 2033 includes a second connection area 92 and a third connection area 93, the second connection area 92 is arranged on a side of the second cladding 601 adjacent to the first sensing portion 2032, and two ends of the second connection area 92 are respectively connected to the second optical fiber 51 and the second cladding 601, the third connection area 93 is arranged on a side of the second cladding 601 adjacent to the second sensing portion 2034, and two ends of the third connection area 93 are respectively connected to the fourth optical fiber 71 and the second cladding 601; the light output portion 2035 includes a fourth connection area 94, which is arranged on a side of the third cladding 801 adjacent to the second sensing portion 2034, and two ends of the fourth connection area 94 are respectively connected to the fourth optical fiber 71 and the third cladding 801.

[0070] Optionally, the first metal film 52 and the second metal film 72 are composed of one or more of a single metal, a mixed alloy, a multi-layer metal composite layer or a semiconductor layer, and have abundant free electrons. The preparation method of the metal film includes but is not limited to depositing it on the surface of the optical fiber by thermal evaporation vacuum coating.

[0071] In one possible example, the surface of the first metal film 52 is modified with a sealing agent layer, the sealing agent layer includes a sealing agent, and the sealing agent layer blocks the first metal film 52 from adsorbing target molecules, so that the refractive index of the surface of the first metal film 52 will not be affected by the adsorption state of the target molecules, but only by other conditions. The surface of the second metal film 72 is modified with a capture functional layer, the capture functional layer includes a target targeting substance for capturing target molecules and a sealing agent, and the sealing agent is used to seal the area of ​​the second metal film 72 where the target targeting substance is not modified, and the refractive index of the surface of the second metal film 72 is affected by the adsorption state of the target molecules and other conditions. Preferably, the target targeting substance of the target molecule is first modified on the second metal film 72, and then sealed with a sealing agent to form a capture functional layer. The modification order of the sealing agent and the target targeting substance on the surface of the second metal film 72 is to modify the target targeting substance first and then seal with a sealing agent.

[0072] Among them, the sealing agent layer can prevent the target molecules from being fixed to the unmodified target target area on the second metal film 72, thereby avoiding the uneven distribution of target molecules adsorbed on the surface of the second metal film 72 and causing detection deviations. The setting of the sealing agent layer can also prevent other molecules in the analyte from being adsorbed to the surface of the second metal film 72 and affecting the accuracy of the detection.

[0073] The components of the blocking agent include but are not limited to bovine serum albumin.

[0074] See also Figure 6 , Figure 6 It is a schematic diagram of a spectral signal provided in an embodiment of the present application, and the target spectral curve corresponding to the target spectral signal is shown in the figure. The ordinate represents the intensity of the emitted light, and the unit is cd candela. The abscissa represents the wavelength of light, and the unit is nm. The target spectral curve includes two trough areas, and the trough area is a bending transition area of ​​the target spectral curve, and the trough area is open along the increasing trend direction of the numerical value of the ordinate; wherein, when the incident light passes through the first sensing portion 2032, a plasma resonance is generated on the surface of the first metal film 52 of the first sensing portion 2032, and the energy of the photons in the first wavelength segment of the incident light is absorbed to form a first trough area of ​​the spectrum of the emitted light. Since the target molecules are adsorbed on the surface of the second metal film 72 of the second sensing portion 2034, the refractive index of the surface of the second metal film 72 increases. When the incident light passes through the second sensing portion 2034, a plasma resonance is generated on the surface of the second metal film 72, and the energy of the photons in the second wavelength segment of the incident light is absorbed to form a second trough area of ​​the spectrum of the emitted light; any wavelength value in the second wavelength segment is greater than the wavelength value in the first wavelength segment.

[0075] As the number of target molecules adsorbed on the surface of the second metal film 72 increases, the refractive index of the surface of the second metal film 72 increases, the second wave valley region moves rightward, and the wavelength of the peak of the second wave valley region increases.

[0076] Among them, the wavelength value corresponding to the peak of the first trough area is the first resonance peak wavelength, and the wavelength value corresponding to the peak of the second trough area is the second resonance peak wavelength. The target variation characterization value is the relative difference between the first resonance peak wavelength and the second resonance peak wavelength or the numerical value of the division operation result; the variation characterization value mapping relationship set includes the corresponding relationship between the variation characterization value and the concentration of the target molecule, and the variation characterization value is positively correlated with the concentration of the target molecule. By determining the target variation characterization value based on the first resonance peak wavelength and the second resonance peak wavelength, the interference of the background liquid is eliminated, which is conducive to improving the accuracy of detection.

[0077] In a possible example, the spectral signal detection system 1 is a single-channel system, that is, the spectral signal detection system 1 is only provided with a single biological detection sensor 203 .

[0078] In a possible example, the spectral signal detection system 1 is a multi-channel system, that is, the spectral signal detection system 1 is provided with a plurality of biological detection sensors 203 for simultaneous detection.

[0079] See also Figure 7 , Figure 7 1 is a schematic diagram of the structure of another spectral signal detection system provided in an embodiment of the present application. The spectral signal detection system 1 comprises a light source 10 , a detection channel 100 , an optical path switch 110 and a spectral detector 30 .

[0080] Optionally, the number of detection channels 100 may be, but is not limited to, two, three, four, five, or other greater numbers.

[0081] Among them, a single detection channel 100 includes a light input section 201, a connecting part 202, a biological detection sensor 203 and a light output section 204. The light output section 204 in each detection channel 100 is connected to the optical path switch 110, the optical path switch 110 is connected to the spectral detector 30, the light input section 201 is connected to the biological detection sensor 203 through the connecting part 202, and the light output section 204 is connected to the biological detection sensor 203 through the connecting part 202.

[0082] The optical path switch 110 is used to switch the detection channel 100 in time-sharing mode, so as to ensure that the detection signal of only one detection channel 100 is transmitted to the spectrum detector 30 at the same time.

[0083] It can be understood that in other embodiments of the present application, multiple connection parts 202 can also be set in an integrated manner, that is, multiple connection parts 202 are set in an integrated optical fiber connection instrument.

[0084] Optionally, a single or multiple light sources 10 are provided in the spectral signal detection system 1 , and a single light source 10 can transmit light to one or more light incident segments 201 , which is not limited in the present application.

[0085] It can be understood that, compared with the comparative embodiment in which a spectral detector 30 is separately configured for each biological detection sensor 203, in this embodiment, the spectral signal detection system 1 collects the detection signals of the output light of different biological detection sensors 203 in a time-sharing manner through the optical path switch 110, and transmits the detection signals of different biological detection sensors 203 to the spectral detector 30 in a time-sharing manner. By controlling the switching speed of the optical path switch 110, it can ensure that the detection signals of each spectral detector 30 can be processed in a timely and effective manner. While ensuring the detection efficiency, the spectral signal detection system 1 can also save costs and reduce the complexity and maintenance difficulty of the system. In addition, since there is no need to set up multiple spectral detectors 30, the spectral signal detection system 1 provided in this embodiment is easy to design in a miniaturized manner, reducing the occupied space of the spectral signal detection system 1, thereby making the application scenarios of the spectral signal detection system 1 more flexible and extensive.

[0086] See also Figure 8 , Figure 8 It is a structural schematic diagram of a spectral signal detection system including an interactometer provided in an embodiment of the present application. The interactometer 120 is connected to the spectral detector 30 in the spectral signal detection system 1. The spectral detector 30 is used to send a target change quantity characterization value to the interactometer 120. The interactometer 120 is used to obtain the target change quantity characterization value from the spectral detector 30, and analyze the strength of the interaction affinity between the target molecule and the target target of the target molecule according to the target change quantity characterization value.

[0087] Among them, the interaction instrument 120 performs biosensor analysis based on the SPR principle, without the need for labeling or purification of various biological components, reducing errors that may be introduced during labeling or purification. The interaction instrument 120 can measure the interaction process between various biomolecules such as peptides, proteins, oligonucleotides, oligosaccharides, as well as viruses, bacteria, cells, and small molecule compounds in real time, in situ, and dynamically under natural conditions through the spectral signal detection system 1, thereby obtaining more realistic and accurate detection results.

[0088] See also Fig. 9 , Fig. 9The present invention is a flow chart of a spectral signal detection method provided by an embodiment of the present application, which is applied to a spectral detector in a spectral signal detection system. The spectral signal detection system includes a light source, an optical fiber path and the spectral detector. The optical fiber path includes a light input section, a connecting part, a biological detection sensor and a light output section. The biological detection sensor includes a light input section, a light output section, a first sensing part, a second sensing part and a light transmission part. The connecting part connects the light input section and the light input section respectively, the connecting part connects the light output section and the light output section respectively, the first sensing part connects the light input section and the light transmission part respectively, and the second sensing part connects the light transmission part and the light output part respectively. The method includes:

[0089] S901, obtaining a target spectral signal of the outgoing light from the biological detection sensor, wherein the target spectral signal is a detection signal of the target molecule in the detection solution adsorbed on the second metal film surface of the second sensing part in a steady state and under other conditions.

[0090] S902, determining a first resonance peak wavelength and a second resonance peak wavelength in the target spectrum signal;

[0091] S903, determining a target variation characterization value according to the first resonance peak wavelength and the second resonance peak wavelength, wherein the target variation characterization value is used to characterize a variation in the absorption capacity of the biosensor to the incident light of the light source under a steady-state constraint when the target molecule is adsorbed on the surface of the second metal film;

[0092] S904: Determine the target concentration of the target molecule according to the target variation characterization value and the variation characterization value mapping relationship set.

[0093] It can be seen that in this example, the signal processing device first collects the target spectral signal of the output light of the biological detection sensor, and determines the first resonance peak wavelength and the second resonance peak wavelength in the target spectral signal, and analyzes the concentration of the target molecule based on the first resonance peak wavelength and the second resonance peak wavelength, thereby eliminating the interference of the background liquid, which is beneficial to improve the accuracy of the detection.

[0094] In one possible example, the interaction instrument is connected to the spectral detector, and the interaction instrument is used to obtain a target change quantity characterization value from the spectral detector, and analyze the strength of the interaction affinity between the target molecule and the target targeting object of the target molecule according to the target change quantity characterization value.

[0095] In a possible example, the light transmission portion is bent, the first sensing portion and the second sensing portion are symmetrically arranged, and the light incident portion and the light emitting portion are symmetrically arranged.

[0096] In a possible example, the incident light is emitted after passing through the light incident part, the first sensing part, the light transmission part, the second sensing part and the light output part of the biological detection sensor in sequence, and the incident light has the same first transmission direction in the light incident part and the light transmission part, and the incident light has the same second transmission direction in the light output part and the light transmission part, and the first transmission direction is opposite to the second transmission direction.

[0097] In a possible example, the light input part includes a first optical fiber, which includes a first cladding and a first core; the first sensing part includes a second optical fiber and a first metal film wrapped on the surface of the second optical fiber, the second optical fiber is connected to the first core, the incident light enters the second optical fiber from the first core, and generates a plasma resonance reaction on the first metal film; the light transmission part includes a third optical fiber, the third optical fiber includes a second cladding and a second core, the second core is connected to an end of the second optical fiber away from the first core, and the incident light enters the second core through the second optical fiber; the second sensing part includes a fourth optical fiber and the second metal film wrapped on the surface of the fourth optical fiber, the fourth optical fiber is connected to an end of the second core away from the second optical fiber, the incident light enters the fourth optical fiber from the second core, and generates a plasma resonance reaction on the second metal film; the light output part includes a fifth optical fiber, the fifth optical fiber includes a third cladding and a third core, the third core is connected to an end of the fourth optical fiber away from the second core, the incident light enters the third core through the fourth optical fiber, and is emitted through the third core to enter the spectrum detector.

[0098] In one possible example, the first metal film is modified with a sealing agent layer, the sealing agent layer includes a sealing agent, and the second metal film is modified with a capture functional layer, the capture functional layer includes a target target for capturing the target molecule and the sealing agent, and the sealing agent is used to seal the area on the second metal film that is not modified with the target target.

[0099] In one possible example, the first optical fiber, the third optical fiber and the fifth optical fiber are multimode optical fibers or single-mode optical fibers; the second optical fiber and the fourth optical fiber are multimode optical fibers or single-mode optical fibers or coreless optical fibers or other optical fiber structures that excite the evanescent field on the optical fiber surface.

[0100] In a possible example, the light incident portion includes a first connection area, the first connection area is arranged on a side of the first cladding adjacent to the first sensing portion, and two ends of the first connection area are respectively connected to the second optical fiber and the first cladding; the light transmission portion includes a second connection area and a third connection area, the second connection area is arranged on a side of the second cladding adjacent to the first sensing portion, and two ends of the second connection area are respectively connected to the second optical fiber and the second cladding, the third connection area is arranged on a side of the second cladding adjacent to the second sensing portion, and two ends of the third connection area are respectively connected to the fourth optical fiber and the second cladding; the light exit portion includes a fourth connection area, the fourth connection area is arranged on a side of the third cladding adjacent to the second sensing portion, and two ends of the fourth connection area are respectively connected to the fourth optical fiber and the third cladding.

[0101] In a possible example, the target variation characterization value is a relative difference between the first resonance peak wavelength and the second resonance peak wavelength or a numerical value of a division operation; the variation characterization value mapping relationship set includes a corresponding relationship between the variation characterization value and the concentration of the target molecule, and the variation characterization value is positively correlated with the concentration of the target molecule.

[0102] See also Fig.10 , Fig.10 Schematic diagram of the structure of a portable optical fiber biological detection device of an embodiment of the present application. The present application also provides a portable optical fiber biological detection device 2, which includes a housing 21, a display screen 22 and a spectral signal detection system 1, wherein the spectral signal detection system 1 is accommodated in the housing 21, and the display screen 22 is disposed outside the housing 21 and is used to display detection information.

[0103] Optionally, the portable optical fiber biological detection device 2 is a handheld device.

[0104] The display screen 22 may be, but is not limited to, the concentration of the target molecule in the detection solution, or the affinity information between the target molecule and the target target of the target molecule, or other characteristic information.

[0105] Understandably, traditional sensors are not conducive to the promotion of handheld devices due to factors such as large size and complex testing process. The portable optical fiber biological detection device 2 provided in this embodiment has a simple structure and a small size, so that the biological detection sensor 203 can be applied to the portable optical fiber biological detection device 2, so that users do not need to carry a bulky detection device, and can perform biological sample detection anytime and anywhere, thereby improving the convenience and flexibility of the detection process.

[0106] The spectral detector provided in this embodiment is used to execute the above-mentioned spectral signal detection method, and thus can achieve the same effect as the above-mentioned implementation method.

[0107] In the case of an integrated unit, the spectrum detector may include a processing module, a storage module and a communication module. The processing module may be used to control and manage the actions of the spectrum detector, for example, it may be used to support the spectrum detector to execute the steps performed by the above functional unit. The storage module may be used to support the spectrum detector to execute stored program codes and data, etc. The communication module may be used to support the communication between the spectrum detector and other devices.

[0108] Among them, the processing module can be a processor or a controller. It can implement or execute various exemplary logic boxes, modules and circuits described in conjunction with the disclosure of this application. The processor can also be a combination that implements computing functions, such as a combination of one or more microprocessors, a combination of digital signal processing (DSP) and a microprocessor, etc. The storage module can be a memory. The communication module can specifically be a device that interacts with other electronic devices, such as a radio frequency circuit, a Bluetooth chip, a Wi-Fi chip, etc.

[0109] An embodiment of the present application also provides a computer storage medium, wherein the computer storage medium stores a computer program for electronic data exchange, wherein the computer program enables a computer to execute part or all of the steps of any method recorded in the above method embodiments, and the above computer includes a spectral detector.

[0110] The present application also provides a computer program product, which includes a non-transitory computer-readable storage medium storing a computer program, and the computer program is operable to cause a computer to execute some or all of the steps of any method described in the method embodiment. The computer program product may be a software installation package, and the computer includes a control platform.

[0111] It should be noted that, for the aforementioned method embodiments, for the sake of simplicity, they are all expressed as a series of action combinations, but those skilled in the art should be aware that the present application is not limited by the described order of actions, because according to the present application, certain steps can be performed in other orders or simultaneously. Secondly, those skilled in the art should also be aware that the embodiments described in the specification are all preferred embodiments, and the actions and modules involved are not necessarily required by the present application.

[0112] In the above embodiments, the description of each embodiment has its own emphasis. For parts that are not described in detail in a certain embodiment, reference can be made to the relevant descriptions of other embodiments.

[0113] In the several embodiments provided in the present application, it should be understood that the disclosed device can be implemented in other ways. For example, the device embodiments described above are only schematic, such as the division of the above-mentioned units, which is only a logical function division. There may be other division methods in actual implementation, such as multiple units or components can be combined or integrated into another system, or some features can be ignored or not executed. Another point is that the mutual coupling or direct coupling or communication connection shown or discussed can be through some interfaces, and the indirect coupling or communication connection of devices or units can be electrical or other forms.

[0114] The units described above as separate components may or may not be physically separated, and the components shown as units may or may not be physical units, that is, they may be located in one place or distributed on multiple network units. Some or all of the units may be selected according to actual needs to achieve the purpose of the solution of this embodiment.

[0115] In addition, each functional unit in each embodiment of the present application may be integrated into one processing unit, or each unit may exist physically separately, or two or more units may be integrated into one unit. The above-mentioned integrated unit may be implemented in the form of hardware or in the form of software functional units.

[0116] If the above-mentioned integrated unit is implemented in the form of a software functional unit and sold or used as an independent product, it can be stored in a computer-readable memory. Based on this understanding, the technical solution of the present application, or the part that contributes to the prior art, or all or part of the technical solution can be embodied in the form of a software product. The computer software product is stored in a memory, including a number of instructions to enable a computer device (which can be a personal computer, server or network device, etc.) to execute all or part of the steps of the above-mentioned methods in each embodiment of the present application. The aforementioned memory includes: U disk, read-only memory (ROM, Read-Only Memory), random access memory (RAM, Random Access Memory), mobile hard disk, disk or optical disk and other media that can store program codes.

[0117] A person of ordinary skill in the art can understand that all or part of the steps in the various methods of the above embodiments can be completed by instructing related hardware through a program, and the program can be stored in a computer-readable memory, which can include: a flash drive, a read-only memory, a random access memory, a magnetic disk or an optical disk, etc.

[0118] The embodiments of the present application are introduced in detail above. Specific examples are used in this article to illustrate the principles and implementation methods of the present application. The description of the above embodiments is only used to help understand the method and core idea of ​​the present application. At the same time, for general technical personnel in this field, according to the idea of ​​the present application, there will be changes in the specific implementation method and application scope. In summary, the content of this specification should not be understood as a limitation on the present application.

Claims

1. A spectral signal detection system, characterized in that: The spectral signal detection system comprises: light source; An optical fiber pathway, the optical fiber pathway comprising a light input section, a connecting portion, a biological detection sensor and a light output section, the biological detection sensor comprising a light input section, a first sensing portion, a second sensing portion, a light output portion and a light transmission portion; one end of the light input section is connected to one end of the light input section through the connecting portion, the light source is located at the other end of the light input section, one end of the light output section is connected to one end of the light output section through the connecting portion, and a spectrum detector is connected to the other end of the light output section; two ends of the first sensing portion are respectively connected to the other end of the light input section and one end of the light transmission portion, and two ends of the second sensing portion are respectively connected to the other end of the light output section and the other end of the light transmission portion; The spectral detector is used to collect a target spectral signal of the outgoing light from the biological detection sensor, determine a first resonance peak wavelength and a second resonance peak wavelength of the target spectral signal, determine a target variation characterization value according to the first resonance peak wavelength and the second resonance peak wavelength, and determine a target concentration of the target molecule according to the target variation characterization value and a set of variation characterization value mapping relationships; the target spectral signal is a detection signal of the target molecule in the detection solution adsorbed on the second metal film surface of the second sensing part under steady state and other condition constraints, and the target variation characterization value is used to characterize the change in the absorption capacity of the biological detection sensor for the incident light of the light source under the steady state constraint when the target molecule is adsorbed on the second metal film surface; The light transmission part is bent, the first sensing part and the second sensing part are symmetrically arranged, and the light input part and the light output part are symmetrically arranged; The light input portion includes a first optical fiber, and the first optical fiber includes a first cladding and a first core; The first sensing part includes a second optical fiber and a first metal film wrapped on the surface of the second optical fiber, the second optical fiber is connected to the first fiber core, the incident light enters the second optical fiber from the first fiber core, and generates a plasma resonance reaction on the first metal film; The light transmission part includes a third optical fiber, the third optical fiber includes a second cladding and a second core, the second core is connected to an end of the second optical fiber away from the first core, and the incident light enters the second core through the second optical fiber; The second sensing part includes a fourth optical fiber and the second metal film wrapped on the surface of the fourth optical fiber, the fourth optical fiber is connected to the end of the second fiber core away from the second optical fiber, the incident light enters the fourth optical fiber from the second fiber core, and generates a plasma resonance reaction on the second metal film; The light output portion includes a fifth optical fiber, the fifth optical fiber includes a third cladding and a third core, the third core is connected to an end of the fourth optical fiber away from the second core, the incident light enters the third core through the fourth optical fiber, and is emitted through the third core and enters the spectrum detector.

2. The system according to claim 1, characterized in that The incident light is emitted after passing through the light incident part, the first sensing part, the light transmission part, the second sensing part and the light output part of the biological detection sensor in sequence, and the incident light has the same first transmission direction in the light incident part and the light transmission part, and the incident light has the same second transmission direction in the light output part and the light transmission part, and the first transmission direction is opposite to the second transmission direction.

3. The system according to claim 1, characterized in that The first metal film is modified with a sealing agent layer, which includes a sealing agent. The second metal film is modified with a capture function layer, which includes a target target that captures the target molecule and the sealing agent. The sealing agent is used to seal the area on the second metal film that is not modified with the target target.

4. The system according to claim 1, characterized in that The first optical fiber, the third optical fiber and the fifth optical fiber are multimode optical fibers or single-mode optical fibers; The second optical fiber and the fourth optical fiber are multimode optical fibers or single-mode optical fibers or coreless optical fibers or other optical fiber structures that excite the evanescent field on the optical fiber surface.

5. The system according to claim 1, characterized in that The light incident portion includes a first connection area, the first connection area is arranged on a side of the first cladding layer adjacent to the first sensing portion, and two ends of the first connection area are respectively connected to the second optical fiber and the first cladding layer; The light transmission portion includes a second connection area and a third connection area, the second connection area is arranged on a side of the second cladding adjacent to the first sensing portion, and two ends of the second connection area are respectively connected to the second optical fiber and the second cladding, the third connection area is arranged on a side of the second cladding adjacent to the second sensing portion, and two ends of the third connection area are respectively connected to the fourth optical fiber and the second cladding; The light emitting portion includes a fourth connecting region, the fourth connecting region is arranged on a side of the third cladding layer adjacent to the second sensing portion, and two ends of the fourth connecting region are respectively connected to the fourth optical fiber and the third cladding layer.

6. The system according to claim 1, characterized in that The target variation characterization value is a relative difference between the first resonance peak wavelength and the second resonance peak wavelength or a division result value; The variation characterization value mapping relationship set includes a corresponding relationship between the variation characterization value and the concentration of the target molecule, and the variation characterization value and the concentration of the target molecule are in a positive correlation relationship.

7. The system according to claim 1, characterized in that The first metal film and the second metal film are composed of one or more of a single metal, a mixed alloy, a multi-layer metal composite layer or a semiconductor layer.

8. A spectral signal detection method, characterized in that: A spectral detector used in a spectral signal detection system as claimed in claim 1, wherein the spectral signal detection system comprises a light source, an optical fiber path and the spectral detector, wherein the optical fiber path comprises a light input section, a connecting part, a biological detection sensor and a light output section, wherein the biological detection sensor comprises a light input section, a light output section, a first sensing part, a second sensing part and a light transmission part, wherein the connecting part respectively connects the light input section and the light input section, wherein the connecting part respectively connects the light output section and the light output section, wherein the first sensing part respectively connects the light input section and the light transmission part, wherein the second sensing part respectively connects the light transmission part and the light output part, wherein the light input section comprises a first optical fiber, wherein the first optical fiber comprises a first cladding and a first core; wherein the first sensing part comprises a second optical fiber and a first metal film wrapped on the surface of the second optical fiber, wherein the second optical fiber is connected to the first core, and wherein the incident light enters the second optical fiber from the first core. The optical fiber comprises a third optical fiber, the third optical fiber comprises a second cladding and a second core, the second core is connected to the end of the second optical fiber away from the first core, and the incident light enters the second core through the second optical fiber; the second sensing part comprises a fourth optical fiber and the second metal film wrapped on the surface of the fourth optical fiber, the fourth optical fiber is connected to the end of the second core away from the second optical fiber, the incident light enters the fourth optical fiber from the second core, and a plasma resonance reaction is generated on the second metal film; the light emitting part comprises a fifth optical fiber, the fifth optical fiber comprises a third cladding and a third core, the third core is connected to the end of the fourth optical fiber away from the second core, the incident light enters the third core through the fourth optical fiber, and is emitted through the third core and enters the spectrum detector; the method comprises: Acquire a target spectral signal of the outgoing light from the biological detection sensor, wherein the target spectral signal is a detection signal of the target molecule in the detection solution adsorbed on the surface of the second metal film of the second sensing part in a steady state and under other conditions; Determine a first resonance peak wavelength and a second resonance peak wavelength in the target spectral signal; Determine a target variation characterization value according to the first resonance peak wavelength and the second resonance peak wavelength, wherein the target variation characterization value is used to characterize a variation in the absorption capacity of the biosensor to the incident light of the light source under steady-state constraints when the target molecule is adsorbed on the surface of the second metal film; The target concentration of the target molecule is determined according to the target variation characterization value and the variation characterization value mapping relationship set.

9. An interaction instrument, characterized in that: The interaction instrument comprises the spectral signal detection system according to any one of claims 1 to 7, and the interaction instrument is used to analyze the strength of the interaction affinity between the target molecule and the target targeting object of the target molecule.

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