A cooling device for taking and placing biological samples in a vacuum

Abstract

The present application provides a cold preservation device for taking and placing biological samples in a vacuum, comprising: a cold preservation seat, with a cold source component attached to the outer wall; a supporting steel cylinder; a heat-insulating connector, comprising a front connecting section and a rear connecting section connected to each other, and having a channel running through it, with one end opening of the cold preservation seat connected to the front connecting section, and the opening of the supporting steel cylinder connected to the rear connecting section; a clamping mechanism, located in the hollow interior of the cold preservation seat, comprising a clamping part and a transmission part, with one end of the clamping part extending out of the other end opening of the cold preservation seat, the clamping part being connected to the cold preservation seat through a rotating pair, and being connected to the transmission part through a moving pair; a power mechanism, comprising a push-pull rod and a motor, the push-pull rod extending from the transmission part through the channel of the heat-insulating connector into the hollow interior of the supporting steel cylinder, and being connected to the output end of the motor. The cold preservation device provided by the present application solves the problems of low transfer efficiency, easy contamination, and easy temperature rise and thawing of biological samples in cryo-electron microscopy.

Description

Technical Field

[0001] The present application relates to the field of transmission equipment for electron microscopes, and in particular to a cooling device for taking and placing biological samples in a vacuum. Background Art

[0002] Cryo-electron microscopy (CEM) is a scanning or transmission electron microscope (TEM) based on ultra-low temperature freezing and transfer technology. It enables direct observation of liquid, semi-liquid, and electron-beam-sensitive biological samples. To facilitate handling and transfer, frozen biological samples obtained using CEM are typically stored in a sample shuttle, which is then transferred to a vacuum chamber. The biological sample is then removed from the shuttle and transferred to the TEM chamber for observation. The CEM process is difficult and inefficient. Transferring biological samples currently relies primarily on manual labor, resulting in low transfer throughput, sample damage and contamination, and difficulty maintaining the frozen state of the biological sample. Summary of the Invention

[0003] In response to the above problems, the present application provides a cold preservation device for taking and placing biological samples in a vacuum, so as to solve the problems of low transfer efficiency, easy contamination and easy thawing of biological samples in cryo-electron microscopy.

[0004] The technical solution of this application is:

[0005] A cold-keeping device for taking and placing biological samples in a vacuum, comprising:

[0006] The cold-keeping seat body is open at both ends and hollow inside, and a cold source component is attached to the outer wall;

[0007] A supporting steel cylinder, open at one end and hollow inside;

[0008] The heat-insulating connector includes a front connecting section and a rear connecting section connected to each other and has a channel running through it. One end opening of the cold-insulating seat is connected to the front connecting section, and the opening of the supporting steel cylinder is connected to the rear connecting section.

[0009] The clamping mechanism is located in the hollow interior of the cold-keeping seat body and includes a clamping portion and a transmission portion. One end of the clamping portion extends out of the opening at the other end of the cold-keeping seat body. The clamping portion is connected to the cold-keeping seat body through a rotating pair and is connected to the transmission portion through a moving pair.

[0010] A power mechanism, comprising a push-pull rod and a motor, wherein the push-pull rod extends from the transmission portion through the hole of the thermal insulation connector into the hollow interior of the supporting steel cylinder and is connected to the output end of the motor;

[0011] Among them, the cold source component transfers the cold energy to the cold preservation seat body, the motor drives the push-pull rod to pull the transmission part to move axially, and the transmission part drives the clamping part to swing in a circle with the rotating pair as the center through the moving pair. The biological sample circle is taken and placed through the movement of the clamping part, and the cold energy stored in the cold preservation seat body is transferred to the biological sample circle.

[0012] Optionally, the outer wall of the cold-insulating seat corresponding to the other end opening is provided with two symmetrical mounting grooves, and a heat conducting sheet is provided in each mounting groove;

[0013] One end of the heat conducting sheet is connected to the wall of the mounting slot, and the other end extends out of the mounting slot and bends toward the clamping portion, and then extends in a direction parallel to the clamping portion.

[0014] Wherein, in the swinging direction of the clamping portion, the extended portion of the heat conducting plate is staggered with the protruding portion of the clamping portion, and the length of the extended portion is smaller than the length of the protruding portion.

[0015] Optionally, the clamping portion includes an upper clamping jaw and a lower clamping jaw, and the upper clamping jaw and the lower clamping jaw each include a clamping head, a rotating middle portion and a moving tail portion connected axially in sequence; wherein,

[0016] The two clamping heads extend out of the other end opening, the two rotating middle parts are connected to the cold-keeping seat body through the rotating pair, and the two moving tail parts are connected to the transmission part through the moving pair;

[0017] The two clamping heads are arranged opposite to each other up and down, the two rotating middle parts are arranged symmetrically front to back, and the two moving tails are arranged opposite to each other front to back and present a symmetrical structure up and down;

[0018] Wherein, the length of the clamping head of the upper clamping jaw is smaller than the length of the clamping head of the lower clamping jaw.

[0019] Optionally, the lower surface of the clamping head of the upper clamping jaw is a plane, and the upper surface of the clamping head of the lower clamping jaw is provided with a clamping hole, and the clamping hole is used to accommodate the biological sample ring.

[0020] Optionally, the two rotating middle parts are respectively provided with a first fixing hole, and the cold-keeping seat body is provided with a second fixing hole;

[0021] The positions of the two first fixing holes are opposite to the position of the second fixing hole;

[0022] A fixing pin passes through the second fixing hole and the two first fixing holes to rotatably connect the cold-keeping seat and the clamping part together;

[0023] The fixing pin, the second fixing hole and the two first fixing holes together constitute the revolving pair.

[0024] Optionally, the two movable tails are respectively provided with a strip-shaped movable hole, and the transmission part is provided with a third fixing hole;

[0025] The positions of the two strip-shaped movable holes are opposite to the position of the third fixed hole;

[0026] A movable pin passes through the third fixing hole and the two strip-shaped movable holes to movably connect the transmission part and the clamping part together;

[0027] The movable pin, the third fixing hole and the two strip-shaped movable holes together constitute the moving pair.

[0028] Optionally, the transmission portion includes a transmission housing, a transmission cavity is defined in the transmission housing, the transmission cavity is used to accommodate the two movable tails, and a through hole is defined at an end of the transmission housing, and one end of the push-pull rod extends into the transmission cavity through the through hole;

[0029] Wherein, an elastic component is provided between the push-pull rod and the transmission housing.

[0030] Optionally, the push-pull rod is connected to the motor via a rotation-stop mechanism;

[0031] The anti-rotation mechanism includes a nut slider, a anti-rotation pin and a nut fixing block, the nut slider is connected to the other end of the push-pull rod, and the nut fixing block is located in the nut slider and connected to the output shaft of the motor;

[0032] The anti-rotation pin passes through the interior of the nut slider and is connected to the supporting steel cylinder.

[0033] Optionally, the cold-keeping seat is made of a heat-conductive material, and the heat-insulating connector is made of a heat-insulating material.

[0034] Optionally, the support steel cylinder is used to connect to an external drive screw through a connecting piece and is installed in a linear bearing; wherein,

[0035] The external driving screw converts its own rotational motion into linear motion of the cold preservation device to drive the cold preservation device to move between the clamping position and the required position, and prevents the movement deviation of the cold preservation device through the linear bearing.

[0036] Compared with the prior art, this application has the following advantages:

[0037] The embodiment of the present application proposes a cold preservation device for taking and placing biological samples in a vacuum, comprising: a cold preservation seat body, which is open at both ends and hollow inside, and a cold source component is attached to the outer wall; a supporting steel cylinder, which is open at one end and hollow inside; a heat-insulating connecting body, comprising a front connecting section and a rear connecting section connected to each other, and having a channel running through it, wherein the opening at one end of the cold preservation seat body is connected to the front connecting section, and the opening of the supporting steel cylinder is connected to the rear connecting section; a clamping mechanism is located in the hollow interior of the cold preservation seat body, and comprises a clamping part and a transmission part, one end of the clamping part extends out of the other end opening of the cold preservation seat body, the clamping part is connected to the cold preservation seat body through a rotating pair, and is connected to the transmission part through a moving pair; a power mechanism, comprising a push-pull rod and a motor, the push-pull rod extending from the transmission part through the channel of the heat-insulating connecting body into the hollow interior of the supporting steel cylinder, and connected to the output end of the motor.

[0038] By adopting the technical solution of the present application, the power mechanism provides a power source, and through the push-pull rod, transmission part, moving pair, rotating pair and clamping part, the rotational motion of the output shaft of the motor is converted into the opening and closing motion of the clamping part, so that the clamping mechanism can grab or put down the biological sample ring according to demand; when the device is in a waiting state, the cold source component is close to the cold-keeping seat to provide cooling for it, ensuring that the cold-keeping seat stores sufficient cold energy, and transfers the cold energy when the clamping mechanism clamps the biological sample ring, ensuring that the biological sample in the biological sample ring is in a frozen state; the thermal insulation connector completely disconnects the cold-keeping seat from direct contact with other metal parts such as the supporting steel cylinder, avoiding the loss of cold energy stored in the cold-keeping seat. Therefore, the biological sample can maintain the required low temperature environment throughout the transfer process, and the integrity, stability and frozen state of the biological sample ring are maintained during the picking and placing process. BRIEF DESCRIPTION OF THE DRAWINGS

[0039] In order to more clearly illustrate the technical solution of the present application, the following is a brief introduction to the drawings required for the description of the present application. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without any creative work.

[0040] Figure 1 This is a schematic diagram of the overall structure of a cold preservation device for taking and placing biological samples in a vacuum according to an embodiment of the present application;

[0041] Figure 2 This is a schematic diagram of the front portion of the heat-insulating connector of the cold-keeping device according to one embodiment of the present application, including the internal structure of the heat-insulating connector;

[0042] Figure 3 This is a schematic diagram of the internal structure of the rear portion of the heat-insulating connector of the cold-keeping device according to one embodiment of the present application;

[0043] Figure 4 This is a three-dimensional structural diagram of a cooling seat equipped with a heat conducting sheet according to an embodiment of the present application;

[0044] Figure 5 is a three-dimensional structural diagram of a clamping mechanism according to an embodiment of the present application;

[0045] Figure 6 This is an overall structural diagram of the clamping mechanism according to one embodiment of the present application when assembled in a cold-keeping seat;

[0046] Figure 7 This is an assembly diagram of components installed on a cold-insulating seat according to an embodiment of the present application;

[0047] Figure 8 This is a schematic diagram of the structure of the cold-insulating seat, the heat-insulating connector, and part of the supporting steel cylinder according to one embodiment of the present application;

[0048] Figure 9 This is a schematic diagram of the assembly of the cold preservation device, linear bearing and external drive screw described in one embodiment of the present application.

[0049] Description of reference numerals:

[0050] 1. Cold-insulating seat; 101. Guide hole; 102. Mounting groove; 2. Heat-insulating connector; 21. Front connecting section; 22. Rear connecting section; 3. Support steel cylinder; 41. Upper clamping jaw; 42. Lower clamping jaw; 421. Clamping hole; 401. Clamping head; 402. Rotating middle part; 403. Moving tail; 5. Transmission part; 51. Transmission housing; 52. Through hole; 6. Heat conducting plate; 7. Fixing pin; 8. Movable pin; 9. Circlip; 10. Push-pull rod; 11. Motor; 111. Output shaft; 12. Nut slider; 13. Stop pin; 14. Nut fixing block; 15. Linear bearing; 16. Screw nut; 17. Screw. DETAILED DESCRIPTION

[0051] The following will be combined with the drawings in the embodiments of this application to clearly and completely describe the technical solutions in the embodiments of this application. Obviously, the embodiments described are part of the embodiments of this application, not all of them. Based on the embodiments in this application, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of this application.

[0052] It should be noted that, compared with traditional electron microscopes, cryo-electron microscopes avoid damaging biological samples by freezing them to extremely low temperatures (usually below liquid nitrogen temperature), so that the structure of the sample can be closer to its natural form. Biological samples can include proteins, protein complexes, cells or organelles, etc., and biological samples are usually fixed in biological sample circles. Several biological sample circles are usually stored in special sample containers, such as sample shuttles. In the electron microscope chamber, the biological sample circle is loaded onto the low-temperature sample stage and then observed by transmission electron microscopy. The image data obtained by the electron microscope is processed and analyzed by special software to obtain the three-dimensional structural information of the biological sample.

[0053] In the related art, in the process of placing the biological sample circle in the sample shuttle and taking it out from the sample shuttle and placing it on the sample stage, special tools such as tweezers are usually used, which requires manual operation. This is not only inefficient, but also easily causes damage or contamination of the sample due to improper operation, and it is difficult to keep the biological sample circle in a frozen state.

[0054] In view of this, please see Figure 1-Figure 3 As shown, Figure 1 The overall structure diagram of the cold preservation device for taking and placing biological samples in a vacuum is shown in the present application; in order to understand its internal structure more clearly, Figure 1 The cooling device shown is divided into two parts. Figure 2 A schematic diagram showing the front portion of the heat-insulating connector of the cold-keeping device and the internal structure thereof; Figure 3 The diagram shows the internal structure of the cold-keeping device after the heat-insulating connector. Figure 1 、 Figure 2 and Figure 3 As shown, the embodiment of the present application provides a cold preservation device for taking and placing biological samples in a vacuum, comprising: a cold preservation seat body 1, which is open at both ends and hollow inside, and a cold source component is attached to the outer wall; a support steel cylinder 3, which is open at one end and hollow inside; a heat-insulating connector 2, comprising a front connecting section 21 and a rear connecting section 22 connected to each other, and having a channel running through it, wherein the opening at one end of the cold preservation seat body 1 is connected to the front connecting section 21, and the opening of the support steel cylinder 3 is connected to the rear connecting section 22; a clamping mechanism, located in the hollow interior of the cold preservation seat body 1, comprising a clamping part and a transmission part 5, one end of the clamping part extends out of the other end opening of the cold preservation seat body 1, the clamping part is connected to the cold preservation seat body 1 through a rotating pair, and is connected to the transmission part 5 through a moving pair; a power mechanism, comprising a push-pull rod 10 and a motor 11, the push-pull rod 10 extends from the transmission part 5 through the channel of the heat-insulating connector 2 into the hollow interior of the support steel cylinder 3, and is connected to the output end of the motor 11;

[0055] Among them, the cold source component transfers the cold energy to the cold-keeping seat body 1, the motor 11 drives the push-pull rod 10 to pull the transmission part 5 to move axially, and the transmission part 5 drives the clamping part to swing in a circle with the rotating pair as the center through the moving pair. The movement of the clamping part realizes the taking and placing of the biological sample circle in the sample shuttle, and transfers the cold energy stored in the cold-keeping seat body 1 to the biological sample circle.

[0056] Specifically, the process of transferring the biological sample ring mainly includes a transmission stage and a pick-and-place stage. The transmission stage is used to transfer the biological sample ring from a clamping position (such as a storage position) to a required position (such as a sample table). In the process of moving the biological sample ring from the clamping position to the required position along a predetermined path, it is usually carried out in a vacuum environment to ensure that the biological sample is not affected by the external environment. The pick-and-place stage involves two steps: sampling and placing. Sampling is the process of grabbing the biological sample ring at the clamping position, and placing is the process of releasing the biological sample ring at the required position.

[0057] This embodiment mainly describes a specific implementation method of automatically taking out a biological sample circle from a sample shuttle, placing the biological sample circle on a sample stage, and keeping the biological sample circle in a frozen state during the taking and placing stage.

[0058] In this embodiment, the whole text is Figure 1 Taking the placement orientation shown as an example, the cold-insulating seat 1, the heat-insulating connector 2, and the supporting steel cylinder 3 are arranged axially from left to right. The three elements share a central axis and have the same outer diameter, and are connected as a whole to form a hollow long rod-like structure. The length of the supporting steel cylinder 3 is greater than that of the cold-insulating seat 1.

[0059] Among them, see Figure 4 , Figure 4 The overall structure of the cold-keeping seat is shown. The cold-keeping seat 1 is a cylindrical structure with two open ends and a hollow interior. The left end opening of the cold-keeping seat 1 serves as a guide hole 101, which is used to support the opening and closing of the extended portion (such as a clamping claw) of the clamping mechanism. The right end opening of the cold-keeping seat 1 serves as a connecting hole, through which the cold-keeping seat 1 is sleeved and connected to the front connecting section 21 of the thermal insulation connector 2. The cylindrical outer wall of the cold-keeping seat 1 serves as a cold source veneer for attaching a cold source component. The cold source component is responsible for providing cooling to the cold-keeping seat 1, thereby maintaining the frozen state of the biological sample ring clamped by the clamping portion within the cold-keeping seat 1.

[0060] The cold retaining seat 1 can be made of a heat-conducting material, preferably a high-conducting material, such as high-purity oxygen-free copper or aluminum, which has a good cooling effect. When the device is in the waiting position, the cold source component is tightly attached to the cold source surface, and the clamping cold seat will store sufficient cold energy. The guide hole 101 cooperates with the clamping mechanism to support the swinging movement of the clamping part and transfer the cold energy to the clamping part.

[0061] In some embodiments, the cold source component is a tubular braided belt cooled by liquid nitrogen and is sleeved and attached to the outer circumference of the cold-keeping seat 1 .

[0062] The support steel cylinder 3 is a hollow cylindrical structure with one end open. It provides support and protection for the entire device. The left end of the support steel cylinder 3 serves as a connecting hole, which connects to the hollow interior. The support steel cylinder 3 is connected to the rear connecting section 22 of the thermal insulation connector 2 through this connecting hole.

[0063] In some embodiments, the support steel cylinder 3 serves as a mounting bracket, the power mechanism and the push-pull rod 10 are installed in the support steel cylinder 3, and the screw nut 16 of the external drive screw is installed on the support steel cylinder 3 through a connecting piece, and the support steel cylinder 3 serves as a moving medium during the transmission process; in some embodiments, the support steel cylinder 3 serves as a guide cylinder. When it is installed in the linear bearing 15, the outer wall of the support steel cylinder 3 and the linear bearing 15 are tolerance matched to ensure that the device is accurately positioned when moving in the linear bearing 15.

[0064] Among them, since the length of the supporting steel cylinder 3 is relatively long, in order to clearly illustrate, Figure 8 The diagram shows the structure of the cold-insulating seat, the heat-insulating connector and part of the supporting steel cylinder. Figure 2 and Figure 8 As shown, the heat-insulating connector 2 is a cylindrical structure connecting the cold-keeping seat 1 and the supporting steel cylinder 3, and a channel passes through the middle area from left to right to form a sleeve with left and right ends open and a channel inside. In this embodiment, the middle part of the cylindrical structure is raised. The middle flange separates the heat-insulating connector 2 into two front connecting sections 21 and rear connecting sections 22 with the same length and the same inner and outer diameters. The outer diameter of the middle flange is the same as the outer diameter of the cold-keeping seat 1 and the supporting steel cylinder 3, and the outer diameters of the front connecting section 21 and the rear connecting section 22 are smaller than the outer diameter of the middle flange. As shown Figure 2 As shown, before the flange of the heat-insulating connector 2 is a front connecting section 21 inscribed in the cold-insulating seat 1, and after the flange is a rear connecting section 22 inscribed in the supporting steel cylinder 3, and the connecting sections are completely covered and shielded by the cold-insulating seat 1 and the supporting steel cylinder 3 respectively.

[0065] like Figure 2As shown, the cold-keeping seat body 1 and the clamping mechanism in the cold-keeping seat body 1, the heat-insulating connecting body 2 and the push-pull rod 10 passing through the heat-insulating connecting body 2 constitute the front part of the device. Figure 3 As shown, the support steel cylinder 3 behind the thermal insulation connector 2 and the anti-rotation mechanism, power mechanism and part of the push-pull rod 10 extending out of the rear connecting section 22 in the support steel cylinder 3 constitute the rear part of the device.

[0066] Specifically, the inner diameter of the connection hole of the cold-insulating seat 1 is adapted to the outer diameter of the front connecting section 21, and the inner diameter of the connection hole of the support steel cylinder 3 is adapted to the outer diameter of the rear connecting section 22. Both are respectively sleeved on the outer circumference of the heat-insulating connector 2 and fastened by two bolts. Specifically, the two bolts sequentially pass through the screw holes of the cold-insulating seat 1 and enter the screw holes of the front connecting section 21, and the two bolts sequentially pass through the screw holes of the support steel cylinder 3 and enter the screw holes of the rear connecting section 22.

[0067] Specifically, the cold-keeping seat 1 and the supporting steel cylinder 3 are isolated by the insulating connector 2, and the insulating connector 2 is made of insulating material, preferably non-metallic plastic, more preferably polyetheretherketone. In a vacuum environment, the direct contact between the cold-keeping seat 1 and other metal parts such as the supporting steel cylinder 3 is completely cut off by the plastic part of the insulating connector 2, thereby avoiding the loss of the cold stored in the cold-keeping seat 1.

[0068] The clamping mechanism, located within the hollow interior of the cold-keeping base 1, consists of a clamping portion and a transmission portion 5. The clamping portion directly contacts the biological sample coil and performs the pick-and-place operation, while the transmission portion 5 transmits the motion of the power mechanism to the clamping portion. One end of the clamping portion extends through the connection hole of the cold-keeping base 1. The clamping portion, located within the cold-keeping base 1, is connected to the cold-keeping base 1 via a revolving pair and to the transmission portion 5 via a moving pair.

[0069] In this embodiment, the revolving pair can be understood as a kinematic pair that allows the clamping portion to rotate relative to the cold-retention base 1. This pair allows the clamping portion to rotate relative to the cold-retention base 1 about a fixed pin 7, but does not allow movement in other directions (such as linear motion). In specific operation, when the transmission portion 5 applies a force via the push-pull rod 10, the clamping portion, under the action of the revolving pair, swings in a circle around the fixed pin 7, thereby grasping or releasing the biological sample ring.

[0070] The moving pair can be understood as a kinematic pair that allows the transmission part 5 to move linearly relative to the clamping part, which allows the transmission part 5 to move linearly relative to the clamping part, but does not allow movement in other directions (such as rotation). In specific operation, since the clamping part is rotationally fixed to the cold-insulating seat body 1 through the rotating pair, when the motor 11 applies a force to the transmission part 5 through the push-pull rod 10, the transmission part 5 moves in a linear direction under the action of the moving pair, pulling the clamping part to have a tendency to move forward or backward. The clamping part is subjected to the force and swings in a circle under the action of the rotating pair, thereby realizing the opening or closing of the clamping part.

[0071] The motor 11 in the power mechanism provides power, and the motor 11 drives the push-pull rod 10, which transmits the power to the transmission part 5 through the push-pull rod 10, and then transmits the power to the clamping part through the transmission part 5, thereby realizing the movement of the clamping mechanism. Figure 2 and Figure 3 As shown, the push-pull rod 10 is simultaneously located in the transmission part 5, the hollow interior of the cold-keeping seat body 1, the hollow interior of the thermal insulation connector 2 and the hollow interior of the support steel cylinder 3. Specifically, the push-pull rod 10 passes through the through hole 52 at the right end of the transmission part 5 from left to right, enters the cold-keeping seat body 1, enters the channel of the thermal insulation connector 2, and passes through the channel into the hollow interior of the support steel cylinder 3, and is connected to the output end of the motor 11 located in the support steel cylinder 3.

[0072] In this embodiment, the inner diameter of the hole should be adapted to the outer diameter of the push-pull rod 10 , so as to allow the push-pull rod 10 to pass through and support the axial movement of the push-pull rod 10 .

[0073] In this embodiment, the distance between the transmission part 5 located in the cold-keeping seat body 1 and the front connecting section 21 connected to the cold-keeping seat body 1 should be greater than the distance that the push-pull rod 10 drives the transmission part 5 to move axially, so as to provide the transmission part 5 with sufficient moving space.

[0074] Through the device provided in the embodiment of the present application, the power mechanism provides a power source, and the rotational motion of the output shaft 111 of the motor 11 is converted into the opening and closing motion of the clamping part through the push-pull rod 10, the transmission part 5, the moving pair, the rotating pair and the clamping part. When the device is in the clamping position or the required position, the clamping mechanism can grab or put down the biological sample ring according to the demand; when the device is in the waiting state, the cold source component is close to the cold preservation seat 1 to provide it with cold, ensuring that the cold preservation seat 1 stores enough cold energy, and transfers the cold energy when the clamping mechanism clamps the biological sample ring, ensuring that the biological sample in the biological sample ring is in a frozen state; the plastic part of the heat-insulating connector 2 completely disconnects the cold preservation seat 1 from direct contact with other metal parts such as the supporting steel cylinder 3, thereby preventing the cold energy stored in the cold preservation seat 1 from being lost.

[0075] Thus, compared to manual operation to remove and place biological sample circles, which has low transfer throughput, complex operation steps, and is prone to sample damage and contamination during the transfer process, the present device enables the removal and placement of biological sample circles from the sample shuttle while maintaining the frozen state of the biological sample circles. This enables automatic sampling and placement operations during the removal and placement phases, improving operational efficiency, repeatability, and reliability. This device allows biological samples to maintain the required low-temperature environment throughout the transfer process, maintaining the integrity, stability, and frozen state of the biological sample circles during the removal and placement process, greatly improving the efficiency and success rate of sample transfer, reducing errors and contamination that may be introduced by manual operation, and facilitating the acquisition of high-quality imaging data. Furthermore, the device has a simple overall structure, is easy to install, and is highly stable, capable of operating in a vacuum environment.

[0076] In summary, the application of the device of the embodiment of the present application in cryo-electron microscopy sample transfer provides strong technical support for the precise processing of samples and maintaining their original state, especially in scenarios requiring highly precise and repeatable sample transfer observation operations, such as structural biology, drug design and materials science, and has important application value.

[0077] To further maintain the frozen state of the biospecimen circle during the transfer process, please continue to refer to Figure 4 Another embodiment of the present application provides that two symmetrical mounting grooves 102 are opened on the outer wall of the cold-keeping seat 1 corresponding to the other end opening, and a heat-conducting plate 6 is arranged in each of the mounting grooves 102; wherein, one end of the heat-conducting plate 6 is connected to the groove wall surface of the mounting groove 102, and the other end extends out of the mounting groove 102 and is bent toward the clamping portion, and then extends in a direction parallel to the clamping portion, and the extension distance is less than the length of the clamping portion extending out of the other end opening; wherein, in the swinging direction of the clamping portion, the extension portion of the heat-conducting plate 6 is staggered with the protruding portion of the clamping portion, and the length of the extension portion is less than the length of the protruding portion.

[0078] Specifically, in conjunction with the above embodiment, the left end opening of the cold-insulating seat 1 is a guide hole 101, and one end of the clamping portion extends out of the guide hole 101. Two mounting slots 102 are defined in the area where the guide hole 101 is located, i.e., the left end portion of the cold-insulating seat 1. Specifically, the left end portion of the cylindrical cold-insulating seat 1 is provided with a U-shaped mounting slot 102 on each of its front and rear sides, each opening toward the left end. The notch of the mounting slot 102 is flush with the left end surface of the cold-insulating seat 1, allowing the U-shaped opening to communicate with the outside world. A rectangular guide hole 101 is defined between the two mounting slots 102.

[0079] A heat conducting plate 6 is installed in the mounting groove 102 by means of bolts, and one end of the clamping portion is at the guide hole 101. Specifically, the bolt passes through the screw hole of the heat conducting plate 6 and enters the screw hole provided on the mounting groove 102. The heat conducting plate 6 includes a connecting portion, a bending portion and an extending portion that are integrally formed. The three are connected as a whole to form a stepped structure. Specifically, the connecting portion of the two heat conducting plates 6 is U-shaped and tightly attached to the bottom wall of the mounting groove 102, and has the same shape and contour as the bottom wall of the groove, and is pressed against the bottom wall of the groove with bolts. The bending portion of the heat conducting plate 6 in the mounting groove 102 on the front side extends in a straight line from front to back, and the bending portion of the heat conducting plate 6 in the mounting groove 102 on the rear side extends in a straight line from back to front, respectively close to the clamping portion in the guide hole 101, and tightly attached to the front end face of the cold preservation seat 1 with the guide hole 101. The starting ends of the bent portions of the two heat conducting plates 6 are connected to the edge of the connecting portion, and the ends are connected to the edge of the extending portion. The extending portion extends straight from the end of the bent portion from right to left, parallel to the extending portion of the clamping portion extending out of the guide hole 101.

[0080] The connection portion secures the heat conducting plate 6 within the mounting groove 102, providing excellent heat transfer and a stable structure that will not loosen or fall off due to operational vibrations. The design of the bent portion ensures that the heat conducting plate 6 can transfer cold energy from the cold-retaining seat 1 to the vicinity of the clamping portion, ensuring that the clamping portion can transfer the biological sample circle in an ultra-low temperature environment, maintaining the low temperature of the biological sample and preventing the biological sample within the biological sample circle from heating up and being damaged. The bent portion brings the extension portion closer to the clamping portion, and the design of the extension portion maximizes heat conduction efficiency, transferring the maximum amount of cold energy to the clamping portion and enhancing the cooling effect.

[0081] In a preferred embodiment, the extension of the thermally conductive sheet 6 is staggered with the protruding portion of the clamping portion in the direction of the clamping portion's swing. This ensures that the thermally conductive sheet 6 does not interfere with the clamping portion's operation while the biological sample remains cooled in the ultra-low temperature environment. For example, the clamping portion swings up and down within the guide hole 101 relative to the cold-retention base 1 to open and close, while the thermally conductive sheet 6 is located on the front and rear sides of the cold-retention base 1. This staggered design ensures that the extension of the thermally conductive sheet 6 does not interfere with the swinging of the clamping portion, allowing the clamping portion to move freely, improving operational precision and reliability.

[0082] In a preferred embodiment, the extension distance of the extension portion is less than the length of the clamping portion extending out of the opening at the other end, that is, the length of the extension portion is less than the length of the protruding portion of the clamping portion, thereby ensuring that the clamping portion has sufficient range of motion when clamping or releasing the biological sample ring in the sample shuttle, thereby preventing the heat conductive sheet 6 from colliding with or interfering with the biological sample ring or the sample shuttle.

[0083] As shown above, the clamping portion can swing up and down in the guide hole 101. The embodiment of this application is used to illustrate the structure of the clamping portion. Figure 5 and Figure 6 , Figure 5 is a schematic diagram of the three-dimensional structure of the clamping mechanism, which shows the overall structure of the clamping part and the transmission part 5. Figure 6 This is a structural diagram of the clamping mechanism when assembled into the cold-keeping seat, wherein the clamping jaws of the clamping portion extend out of the cold-keeping seat 1. The clamping portion includes an upper clamping jaw 41 and a lower clamping jaw 42, each of which includes a clamping head 401, a rotating middle portion 402, and a movable tail portion 403 connected axially in sequence; wherein the two clamping heads 401 extend out of the other end opening, the two rotating middle portions 402 are connected to the cold-keeping seat 1 via the rotating pair, and the two movable tail portions 403 are connected to the transmission portion 5 via the movable pair; wherein the two clamping heads 401 are arranged opposite to each other in upper and lower positions, the two rotating middle portions 402 are arranged symmetrically front to back, and the two movable tail portions 403 are arranged opposite to each other in upper and lower positions and form a symmetrical structure; wherein the length of the clamping head 401 of the upper clamping jaw 41 is less than the length of the clamping head 401 of the lower clamping jaw 42.

[0084] Specifically, the clamping portion includes an upper clamping jaw 41 and a lower clamping jaw 42. Each clamping jaw is composed of a clamping head 401, a rotating middle portion 402, and a moving tail portion 403 arranged from left to right. The clamping head 401 extends out of the guide hole 101 for directly grabbing and placing the biological sample ring. In combination with the above embodiment, the protruding portion of the clamping portion can be understood as the clamping head 401. Figure 5 As shown, the clamping heads 401 of the upper and lower jaws differ. The upper surface of the clamping head 401 of the lower jaw 42 features a clamping hole 421 to accommodate the biological sample coil. The clamping hole 421 can adapt to the shape and contour of the biological sample coil, improving stability and safety during transfer. The lower surface of the clamping head 401 of the upper jaw 41 is flat and slightly shorter than that of the lower jaw 42, making it easier to grip the biological sample coil. The upper and lower jaws 41 and 42 share the same rotating middle portion 402, while the movable tail portions 403 have the same structure but face opposite directions.

[0085] Specifically, the clamping jaws are an up / down flip-jointed structure connected by a rotating pair and a movable pair, allowing the clamping head 401 to mate with the clamping position inside the sample shuttle. Taking the upper clamping jaw 41 as an example, it is a downward flip-jointed structure, with the main surface of the clamping head 401 facing downward. The rotating middle portion 402 flips downward from the end of the clamping head 401, with its main surface facing forward. The movable tail portion 403 is connected to the rotating middle portion 402, with its main surface also facing forward and tilted upward. Taking the lower clamping jaw 42 as an example, it is an upward flip-jointed structure, with the main surface of the clamping head 401 facing upward. The rotating middle portion 402 flips upward from the end of the clamping head 401, with its main surface facing backward. The movable tail portion 403 is connected to the rotating middle portion 402, with its main surface also facing backward and tilted downward.

[0086] The main surface is the side of the corresponding component with the largest size.

[0087] In this way, the up / down flipping and splicing structure makes the entire device more compact in structure, saves space, and is conducive to the sampling and setting out operations in a limited operating space.

[0088] In some embodiments, the front and rear positions of the two jaws can be interchanged.

[0089] More specifically, the distance between the rotating middle portion 402 of the upper clamping jaw 41 and the front edge of its clamping head 401 is the same as the distance between the rotating middle portion 402 of the lower clamping jaw 42 and the rear edge of its clamping head 401. The distance between the rear edge of the rotating middle portion 402 of the upper clamping jaw 41 and the front edge of the rotating middle portion 402 of the lower clamping jaw 42 is the same. This ensures that the clamping heads 401 of the upper and lower clamping jaws 41 and 42 face each other vertically, and their rotating middle portions 402 are symmetrical front-to-back within the cold-keeping seat 1. Furthermore, the movable tail portions 403 are also opposed to each other in the front-to-back direction, but with different tilt directions.

[0090] The rotating middle parts 402 of the two are symmetrical front to back in the cold preservation seat body 1. Therefore, in the symmetrical area, a first fixing hole is respectively opened in the rotating middle parts 402 of the upper clamping jaw 41 and the lower clamping jaw 42, and a second fixing hole is opened in the cold preservation seat body 1. The positions of the two first fixing holes are respectively opposite to the positions of the second fixing holes. A fixing pin 7 is passed through the second fixing hole on the front side of the cold preservation seat body 1, the first fixing hole on the rotating middle part 402 of the upper clamping jaw 41, the first fixing hole on the rotating middle part 402 of the lower clamping jaw 42, and the second fixing hole on the rear side of the cold preservation seat body 1 in sequence, thereby forming a rotating pair. The cold preservation seat body 1 and the upper clamping jaw 41 and the lower clamping jaw 42 are rotatably connected through the rotating pair, so that the upper clamping jaw 41 and the lower clamping jaw 42 rotate relative to the cold preservation seat body 1 around the fixing pin 7 to realize the opening and closing of the clamping part.

[0091] In combination with the above embodiment, the second fixing hole is provided in the installation slot 102 and is adjacent to the screw hole on the installation slot 102 .

[0092] More specifically, the two movable tails 403 are respectively provided with a strip-shaped movable hole, and the transmission part 5 is provided with a third fixed hole; the positions of the two strip-shaped movable holes are opposite to the position of the third fixed hole; the movable pin 8 passes through the third fixed hole and the two strip-shaped movable holes to movably connect the transmission part 5 and the clamping part together; wherein, the movable pin 8, the third fixed hole and the two strip-shaped movable holes together constitute the movable pair.

[0093] The two movable tails 403 of the two clamping jaws are each provided with a strip-shaped movable hole, which allows the movable pin 8 to move freely along the long axis of the hole. The shape of the strip-shaped movable hole corresponds to the shape of the movable tail 403. That is, the strip-shaped movable hole of the movable tail 403 of the upper clamping jaw 41 is inclined upward, while the strip-shaped movable hole of the movable tail 403 of the lower clamping jaw 42 is inclined downward.

[0094] The transmission portion 5 is provided with a third fixing hole, which is positioned corresponding to the two strip-shaped movable holes in the clamping portion. The third fixing hole is used to fix the movable pin 8, but allows the movable pin 8 to move within the strip-shaped movable hole. The movable pin 8 passes through the third fixing hole on the front side of the transmission portion 5, the strip-shaped movable hole in the movable tail 403 of the upper clamping jaw 41, the strip-shaped movable hole in the movable tail 403 of the lower clamping jaw 42, and the third fixing hole on the rear side of the transmission portion 5, thereby forming a revolving pair, connecting the transmission portion 5 and the clamping portion together, and allowing the transmission portion 5 to move in a linear direction under the drive of the push-pull rod 10.

[0095] In conjunction with the above embodiments, during actual operation, the two clamping heads 401 can cooperate with the clamping positions within the sample shuttle. The first and second fixing holes allow the fixed pin 7 to pass through. The fixed pin 7 rotates but cannot move within the fixed holes. The movable pin 8 passes through the strip movable hole and the third fixing hole. The movable pin 8 is connected to the transmission part 5 and can slide and translate with the transmission part 5 within the strip movable hole. The motor 11 drives the transmission part 5 to move the movable pin 8 back and forth. As the movable pin 8 moves within the two strip movable holes, it uses the principle of leverage to drive the two clamping jaws to swing in a circle around the fixed pin 7. The two clamping heads 401 cooperate with the clamping positions of the sample ring within the sample shuttle to facilitate the placement and retrieval of biological samples.

[0096] Specifically, when the output shaft 111 of the motor 11 drives the push-pull rod 10 to move to the right, the push-pull rod 10 drives the transmission part 5 and the movable pin 8 on the transmission part 5 to move to the right in the bar-shaped movable hole. Since the bar-shaped movable hole of the upper clamping jaw 41 is tilted upward and the bar-shaped movable hole of the lower clamping jaw 42 is tilted downward, when the movable pin 8 moves to the right in the bar-shaped movable hole, pressure is applied to the hole walls of the two bar-shaped movable holes through the movable pin 8. Due to the lever principle, the upper clamping jaw 41 swings upward clockwise with the fixed pin 7 as the center relative to the cold preservation seat 1, and the lower clamping jaw 42 swings downward counterclockwise with the fixed pin 7 as the center relative to the cold preservation seat 1. The clamping part opens and puts the grabbed sample ring at the required position to achieve sample placement. Similarly, when sampling at the clamping position, based on the opposite steps, the two clamping jaws are closed to grab the biological sample ring. When sampling or placement is completed, the clamping mechanism returns to the waiting state and prepares for the next operation.

[0097] For further explanation of this example, please refer to Figure 5, and refer to Figure 7 , Figure 7 This is the assembly drawing of the parts installed on the cold insulation seat. Figure 7 The cold-insulation seat 1 is removed, revealing the clamping mechanism within the cold-insulation seat 1, the front connecting section 21 of the heat-insulating connector 2, the portion of the push-pull rod 10 that passes through the front connecting section 21, and the heat-conducting plate 6 located on the mounting slot 102 of the cold-insulation seat 1. The transmission portion 5 includes a transmission housing 51, which defines a transmission cavity for accommodating the two movable tails 403. The transmission housing 51 also defines a through hole 52 at its end, through which one end of the push-pull rod 10 extends into the transmission cavity. An elastic member is disposed between the push-pull rod 10 and the transmission housing 51.

[0098] In this embodiment, the transmission portion 5 has a structure with a transmission cavity. Its left end is open, through which the movable tails 403 of the two clamping jaws enter the transmission cavity. The right end is provided with a through hole 52, through which the left end of the push-pull rod 10 extends into the transmission cavity. The opening at the left end extends through both the upper and lower sides, and the transmission cavity also extends through both the upper and lower sides. This embodiment simplifies the overall structure of the transmission portion 5, facilitates the axial movement of the movable pin 8 under the force of the push-pull rod 10, and adapts to different loads and operating conditions, thereby improving its adaptability and flexibility.

[0099] Preferably, an elastic component is disposed between the push-pull rod 10 and the transmission housing 51. The elastic component may include a retaining spring 9. The left end of the push-pull rod 10 extends into the transmission cavity through the through hole 52. Preferably, retaining springs 9 are disposed on the left and right sides of the through hole 52, i.e., on the inner and outer walls of the right end of the transmission housing 51, respectively, to secure the position between the push-pull rod 10 and the transmission housing 51 and ensure stability between the push-pull rod 10 and the transmission housing 51 during movement.

[0100] In combination with the above embodiments, in another technical solution, the push-pull rod 10 is connected to the motor 11 through a stop mechanism; the stop mechanism includes a nut slider 12, a stop pin 13 and a nut fixing block 14, the nut slider 12 is connected to the other end of the push-pull rod 10, the nut fixing block 14 is located in the nut slider 12, and is connected to the output shaft 111 of the motor 11; the stop pin 13 passes through the interior of the nut slider 12 and is connected to the supporting steel cylinder 3.

[0101] In this embodiment, the right end of the push-pull rod 10 is connected to the left end of the nut slider 12. A nut fixing block 14 is provided inside the right end of the nut slider 12. The nut fixing block 14 is fixed to the output shaft 111 of the motor 11. The power of the motor 11 is transmitted to the nut slider 12 through the nut fixing block 14, driving the push-pull rod 10 to move. The anti-rotation pin 13 passes through the interior of the nut slider 12 and is connected to the supporting steel cylinder 3, ensuring that the nut fixing block 14 and the nut slider 12 do not rotate circumferentially and only move linearly. The forward and backward movement of the nut fixing block 14 and the nut slider 12 is achieved through the output shaft 111 of the motor 11, thereby achieving axial linear motion of the push-pull rod 10, and then driving the transmission part 5 to make the movable shaft move axially within the bar-shaped movable hole.

[0102] In combination with the above embodiments, in a preferred embodiment, the supporting steel cylinder 3 is used to be connected to an external driving screw through a connecting piece and is installed in a linear bearing 15; wherein, the external driving screw converts its own rotational motion into the linear motion of the cold preservation device to drive the cold preservation device to move between the clamping position and the required position, and prevents the movement of the cold preservation device from deviating through the linear bearing.

[0103] Specifically, in the process of transferring the biological sample circle, the embodiment of the present application can automatically transfer during the transfer phase. Figure 9 As shown, Figure 9 The structure of the cold preservation device is shown, mounted on an external drive screw and positioned within a linear bearing 15. The support cylinder 3 can be connected to the external drive screw via a connector and mounted within the linear bearing 15. Multiple linear bearings 15 can be provided along the length of the support cylinder 3. Driven by the external drive screw, the support cylinder 3 can reciprocate within the linear bearings 15, ensuring that the external drive screw can automatically transport the biological sample coil to the desired location.

[0104] The external drive screw includes an external power source, a screw 17, and a screw nut 16. The external power source can be a drive motor. The screw nut 16 is connected to the support steel cylinder 3 via a connector. The screw nut 16 is connected to the screw 17 and is connected to the output end of the drive motor. When the drive motor rotates, it drives the screw 17 to rotate, and the screw 17 transmits force to the screw nut 16, which moves along the axial direction of the screw 17. The screw nut 16 is connected to the support steel cylinder 3, driving the support steel cylinder 3 to transmit linearly, thereby converting the rotational motion of the external drive screw into axial reciprocating motion of the cold preservation device, allowing the cold preservation device to move quickly and accurately between different positions, improving operational efficiency. Because the support steel cylinder 3 is limited within multiple linear bearings 15, the cold preservation device is prevented from deviating during movement, ensuring that it always moves along the predetermined linear track. In summary, the combination of the transfer stage and the placement stage ensures that the biological sample ring is always in an automatic state throughout the transfer process, helping to protect the sample and improve operational efficiency.

[0105] The steps for using a cooling device for taking and placing biological samples in a vacuum provided in an embodiment of the present application are as follows:

[0106] 1. Pass the support steel cylinder 3 in the cold preservation device through the inner holes of multiple linear bearings 15. The support steel cylinder 3 can be fixed together with the screw nut 16 in the external drive screw using a connector;

[0107] 2. The cold-keeping device is in the waiting position, and the cold source component is tightly attached to the cold source surface of the cold-keeping seat 1, supplying cold to the cold-keeping seat 1, so that the cold-keeping seat 1 has sufficient storage cold capacity;

[0108] 3. The external driving screw drives the cold preservation device to move forward linearly under the limit of the linear bearing 15, so that the clamping part in the cold preservation device moves to the clamping position;

[0109] 4. The power mechanism drives the clamping mechanism to clamp the biological sample circle tightly;

[0110] 5. The external driving screw drives the cooling device to move linearly backward under the limit of the linear bearing 15, and transfers the biological sample circle to the required position;

[0111] 6. The power mechanism drives the clamping mechanism to place the biological sample circle at the required position;

[0112] 7. The cold keeping device returns to the waiting position.

[0113] It should be noted that the various embodiments in this specification are described in a progressive manner, and each embodiment focuses on the differences from other embodiments. The same and similar parts between the various embodiments can be referenced to each other.

[0114] It should also be noted that, in this article, the directions or positional relationships indicated by the terms "upper", "lower", "left", "right", "inside", "outside", etc. are based on the directions or positional relationships shown in the accompanying drawings, and are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific direction, be constructed and operated in a specific direction, and therefore cannot be understood as a limitation on this application. In addition, relational terms such as "first" and "second" are merely used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply that there is any such actual relationship or order between these entities or operations, nor can they be understood as indicating or implying relative importance. Moreover, the term "include" or any other variant thereof is intended to cover non-exclusive inclusion, so that a process, method, article or terminal device that includes a series of elements includes not only those elements, but also includes other elements that are not explicitly listed, or also includes elements that are inherent to such process, method, article or terminal device.

[0115] The above describes in detail the cooling device for placing and retrieving biological samples in a vacuum environment provided by this application. Specific examples are used herein to illustrate the principles and implementation methods of this application. The description of the above examples is intended only to facilitate understanding of this application, and the contents of this specification should not be construed as limiting this application. Furthermore, those skilled in the art will appreciate that various modifications may be made to the specific implementation methods and scope of application based on this application. While it is not necessary and impossible to exhaustively enumerate all implementation methods here, any obvious variations or modifications arising therefrom remain within the scope of protection of this application.

Claims

1. A cooling device for taking and placing biological samples in a vacuum, characterized in that: include: The cold-keeping seat body is open at both ends and hollow inside, and a cold source component is attached to the outer wall; A supporting steel cylinder, open at one end and hollow inside; The heat-insulating connector includes a front connecting section and a rear connecting section connected to each other and has a channel running through it. One end opening of the cold-insulating seat is connected to the front connecting section, and the opening of the supporting steel cylinder is connected to the rear connecting section. The clamping mechanism is located in the hollow interior of the cold-keeping seat body and includes a clamping portion and a transmission portion. One end of the clamping portion extends out of the opening at the other end of the cold-keeping seat body. The clamping portion is connected to the cold-keeping seat body through a rotating pair and is connected to the transmission portion through a moving pair. A power mechanism, comprising a push-pull rod and a motor, wherein the push-pull rod extends from the transmission portion through the hole of the thermal insulation connector into the hollow interior of the supporting steel cylinder and is connected to the output end of the motor; Among them, the cold source component transfers the cold energy to the cold preservation seat body, the motor drives the push-pull rod to pull the transmission part to move axially, and the transmission part drives the clamping part to swing in a circle with the rotating pair as the center through the moving pair. The biological sample circle is taken and placed through the movement of the clamping part, and the cold energy stored in the cold preservation seat body is transferred to the biological sample circle.

2. A cooling device for taking and placing biological samples in a vacuum according to claim 1, characterized in that: The outer wall of the cold-keeping seat corresponding to the other end opening is provided with two symmetrical mounting grooves, each of which is provided with a heat conducting sheet; One end of the heat conducting sheet is connected to the wall of the mounting slot, and the other end extends out of the mounting slot and bends toward the clamping portion, and then extends in a direction parallel to the clamping portion. Wherein, in the swinging direction of the clamping portion, the extended portion of the heat conducting plate is staggered with the protruding portion of the clamping portion, and the length of the extended portion is smaller than the length of the protruding portion.

3. The cold preservation device for taking and placing biological samples in a vacuum according to claim 1, characterized in that: The clamping portion includes an upper clamping jaw and a lower clamping jaw, and each of the upper clamping jaw and the lower clamping jaw includes a clamping head, a rotating middle part and a moving tail part connected in axial sequence; wherein, The two clamping heads extend out of the other end opening, the two rotating middle parts are connected to the cold-keeping seat body through the rotating pair, and the two moving tail parts are connected to the transmission part through the moving pair; The two clamping heads are arranged opposite to each other up and down, the two rotating middle parts are arranged symmetrically front to back, and the two moving tails are arranged opposite to each other front to back and present a symmetrical structure up and down; Wherein, the length of the clamping head of the upper clamping jaw is smaller than the length of the clamping head of the lower clamping jaw.

4. The cold preservation device for taking and placing biological samples in a vacuum according to claim 3, characterized in that: The lower surface of the clamping head of the upper clamping jaw is a plane, and the upper surface of the clamping head of the lower clamping jaw is provided with a clamping hole, and the clamping hole is used to accommodate the biological sample ring.

5. The cold preservation device for taking and placing biological samples in a vacuum according to claim 3, characterized in that: The two rotating middle parts are respectively provided with a first fixing hole, and the cold-keeping seat body is provided with a second fixing hole; The positions of the two first fixing holes are opposite to the position of the second fixing hole; A fixing pin passes through the second fixing hole and the two first fixing holes to rotatably connect the cold-keeping seat and the clamping part together; The fixing pin, the second fixing hole and the two first fixing holes together constitute the revolving pair.

6. The cold preservation device for taking and placing biological samples in a vacuum according to claim 3, characterized in that: The two movable tail parts are respectively provided with strip-shaped movable holes, and the transmission part is provided with a third fixing hole; The positions of the two strip-shaped movable holes are opposite to the position of the third fixed hole; A movable pin passes through the third fixing hole and the two strip-shaped movable holes to movably connect the transmission part and the clamping part together; The movable pin, the third fixing hole and the two strip-shaped movable holes together constitute the moving pair.

7. The cold preservation device for taking and placing biological samples in a vacuum according to claim 6, characterized in that: The transmission part includes a transmission housing, a transmission cavity is defined in the transmission housing, the transmission cavity is used to accommodate the two moving tails, and a through hole is defined at the end of the transmission housing, and one end of the push-pull rod extends into the transmission cavity through the through hole; Wherein, an elastic component is provided between the push-pull rod and the transmission housing.

8. The cold preservation device for taking and placing biological samples in a vacuum according to claim 1, characterized in that: The push-pull rod is connected to the motor via a rotation-stop mechanism; The anti-rotation mechanism includes a nut slider, a anti-rotation pin and a nut fixing block, the nut slider is connected to the other end of the push-pull rod, and the nut fixing block is located in the nut slider and connected to the output shaft of the motor; The anti-rotation pin passes through the interior of the nut slider and is connected to the supporting steel cylinder.

9. The cold preservation device for taking and placing biological samples in a vacuum according to claim 1, characterized in that: The cold-keeping seat is made of heat-conducting material, and the heat-insulating connector is made of heat-insulating material.

10. The cold preservation device for taking and placing biological samples in a vacuum according to claim 1, characterized in that: The support steel cylinder is used to connect with the external driving screw through a connecting piece and is installed in a linear bearing; wherein, The external driving screw converts its own rotational motion into linear motion of the cold preservation device to drive the cold preservation device to move between the clamping position and the required position, and prevents the movement deviation of the cold preservation device through the linear bearing.

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