A plant composition with anti-aging and protective effects and its application

Abstract

The present invention relates to the field of cosmetics, and in particular to a plant composition with anti-aging and protective effects and its application. The plant composition with anti-aging and protective effects comprises the following components in parts by weight: 5-13 parts of kudzu vine extract, 15-35 parts of Shennong chrysanthemum extract, 1-10 parts of scutellaria extract, 5-11 parts of sophora japonica extract, 5-13 parts of milk thistle extract, and 1-10 parts of sunflower extract. The present invention starts from multiple approaches such as resisting ultraviolet rays and protecting during sun exposure, and compounding six kinds of plant extracts to obtain a plant composition. The multi-component synergistic effect has the characteristics of being natural and safe, and also has the effects of protecting during sun exposure, soothing after sun exposure, resisting ultraviolet rays, and anti-aging, and can be used to prepare cosmetics.

Description

Technical Field

[0001] The present invention relates to the field of cosmetics, and in particular to a plant composition with anti-aging and protective effects and application thereof. Background Art

[0002] Ultraviolet rays are the 200-400nm part of the solar spectrum, and are generally divided into three zones according to the length of the wavelength: Zone A (long-wave ultraviolet): 320nm-400nm; Zone B (medium-wave ultraviolet): 280nm-320nm; Zone C (short-wave ultraviolet): 200nm-280nm. The ultraviolet rays in Zones B and A have physiological effects on human skin. Most of the medium-wave ultraviolet radiation is absorbed by the epidermis, and a small amount reaches the dermis. UVB radiation has a short wavelength and high energy. It can pass through the entire epidermis and the epidermal basement membrane of human skin, inducing pigmentation and causing sunburn. At the same time, excessive UVB will cause inflammation in damaged cells, hyperdilation of capillaries, and non-specific acute inflammatory reactions, such as erythema and blisters. The skin barrier function is manifested by increased TEWL, decreased water content, and increased erythema index. Relevant studies have shown that when a single exposure to a large amount of UVB radiation or a long-term exposure to UVB radiation, it will significantly affect the skin's multilayer structure and interfere with the skin barrier. Most of the long-wave ultraviolet radiation in zone A penetrates the dermis, and a small amount penetrates the subcutaneous tissue. The radiation penetration ability is much greater than that of ultraviolet rays in zone B, and it can also penetrate most transparent glass and plastics. UVA will be refracted into the room, also known as (indoor ultraviolet rays), which can penetrate the surface of the skin and go deep into the tissue below the dermis. After the skin is irradiated by UVA, it induces the production of ROS, which leads to the oxidation of nucleic acids and proteins and extensive cell damage and death, indirectly causing DNA damage, and can destroy collagen, elastic fiber tissue and other micro-structures inside the skin, thereby causing skin sagging and wrinkles; UVA will also oxidize the reduced melanin in the epidermis and directly tan the skin, which will cause skin photoaging over time, causing skin sagging and fine lines.

[0003] Unprotected skin will experience a series of UV effects after being exposed to UV radiation, such as sunburn erythema, skin darkening, photoaging, etc. The occurrence of these photodamage mechanisms is closely related to the dose of UV radiation, free radical-induced inflammatory response, etc. At present, the main ways to reduce skin photodamage are: adding physical sunscreens or chemical sunscreens to photoprotective products. Physical sunscreens mainly work by refraction and reflection of UV rays, such as the more commonly used nano titanium dioxide and nano zinc oxide. These sunscreens themselves have photocatalytic activators and are irritating. They are used in large quantities and are more likely to cause false white spots and clog pores. Chemical sunscreens are organic compounds that mainly selectively absorb UV rays to play a role in sun protection, such as homosalate, benzophenone-3, ethylhexyl salicylate, etc. Most of them are also irritating and photosensitizing substances. If added in too high a concentration, there is a possibility of obvious irritation or allergies.

[0004] CN109303748A discloses a natural plant sunscreen composition, a preparation method thereof and an application of the composition in sunscreen cosmetics. The sunscreen composition is prepared from water, glycerin, butylene glycol, ethanol, nano hemp stalk core powder, betaine, trehalose, kudingcha extract, scutellaria baicalensis extract, lespedeza extract, safflower extract, and sophora japonica extract. The composition has a protective effect against both UVA and UVB, but does not demonstrate its effects of scavenging free radicals, repairing after sun exposure, and moisturizing.

[0005] CN113855614B discloses a sunscreen composition and its preparation method and application, the sunscreen composition comprising the following components: snow lotus extract, sea buckthorn fruit extract, scutellaria root extract, Tibetan calamus extract, milk thistle seed extract, water. It has a good sunscreen effect, can remove free radicals and inhibit tyrosinase activity, but does not have a soothing effect.

[0006] With the pollution of the environment and the destruction of the global atmosphere, more ultraviolet rays and high-energy blue light are irradiated on the ground surface. Various harmful lights cause certain harm to the human body, and people are paying more and more attention to sun protection. Traditional hard sunscreen is not convenient to use, and people's demand for sunscreen skin care products with sunscreen effect is increasing. Common sunscreen skin care products are generally physical or chemical sunscreen. Physical sunscreen is generally thicker in texture, prone to whitening and card lines, and there are problems such as irritation and easy clogging of pores. Traditional chemical sunscreens have some safety hazards, such as irritation to the human body and certain pollution to plants or the marine environment. Few raw materials can resist ultraviolet rays and have soothing and anti-aging effects. Therefore, it is urgent to develop a sunscreen skin care product with protection during sun exposure, soothing after sun exposure, synergistic protection and certain anti-aging effects. Summary of the invention

[0007] The purpose of the present invention is to overcome the shortcomings of the prior art and provide a plant composition with anti-aging and protective effects and its application in protective products.

[0008] To achieve the above object, the technical solution adopted by the present invention is:

[0009] In the first aspect, the present invention provides a plant composition with anti-aging and protective effects, comprising the following components in parts by weight: 5-12 parts of kudzu vine extract, 15-34 parts of Shennong chrysanthemum extract, 1-10 parts of scutellaria baicalensis extract, 5-11 parts of sophora japonica extract, 5-13 parts of milk thistle extract, and 1-10 parts of sunflower extract.

[0010] Gexianmi is a pseudo-globose genus of Nostoc, a wild freshwater algae belonging to the Nostoc family of the Cyanobacteria class. It is rich in protein and polysaccharides. In traditional Chinese medicine, Gexianmi is sweet, mild and cold in nature. The plant raw materials of Shennong Xiangju extract come from Shennong Xiangju in the Shennongjia area, which is a variant of the genus Chrysanthemum in the Asteraceae family. The main chemical components of Shennong Xiangju are flavonoids and ethyl acetate compounds, which have good free radical scavenging ability and good protective effect on tissues. Scutellaria baicalensis is a plant of the Lamiaceae family, bitter in taste and cold in nature, with the effects of clearing heat and dampness, purging fire and detoxifying. Baicalin is rich in flavonoid compounds and has certain antioxidant and anti-radiation effects. Sophora japonica is the dried flower bud of a deciduous tree of the Leguminosae family. Shennong's Herbal Classic also lists it as a top grade. It tastes bitter and slightly cold, and has the effects of clearing the liver and purging fire. In recent years, many daily chemical and medical workers have studied its effects and found that it contains active ingredients such as rutin, quercetin, and triterpenoid saponins, and has analgesic, antibacterial, anti-aging, and anti-free radical effects. Milk thistle is the dried mature fruit of the Asteraceae plant Milk thistle, which has the effects of clearing away heat and detoxifying, soothing the liver and promoting gallbladder. The chemical components of milk thistle mainly include silymarin, silymarin protein and other components, among which silymarin is one of the main functional substances of milk thistle, belonging to flavonoid compounds, and has the functions of anti-oxidation, regulating lipid and sugar metabolism and resisting light aging. The present invention compounded the extract of Pueraria lobata, the extract of Shennong Xiangju, the extract of Scutellaria baicalensis, the extract of Sophora japonica, the extract of Milk thistle and the extract of Sunflower to obtain a plant composition. In the plant composition, the extract of Puerariae lobatae has the effects of moisturizing, moisturizing and repairing; the extract of Shennong Xiangju can soothe after sun exposure; the extract of Scutellaria baicalensis can play a certain role in sun protection; at the same time, after compounding with the extract of Sophora japonicae, the extract of Milk thistle and the extract of Helianthus annuus, the plant extract can strengthen the role of the plant extract in photoaging and photoprotection, play a certain role in resisting ultraviolet rays, reduce the damage of ultraviolet rays to the skin, and can also remove free radicals generated by UVB irradiation, reduce DNA damage, and soothe the inflammatory symptoms caused by sunburn. The plant composition can be used to prepare sunscreen, soothing and anti-photoaging products.

[0011] Preferably, the plant composition with anti-aging and protective effects comprises the following components in parts by weight: 9-12 parts of kudzu vine extract, 15-34 parts of Shennong chrysanthemum extract, 5-10 parts of scutellaria baicalensis extract, 10-11 parts of sophora japonica extract, 9-13 parts of milk thistle extract, and 8-10 parts of sunflower extract.

[0012] More preferably, the plant composition with anti-aging and protective effects comprises the following components in parts by weight: 9 parts of kudzu vine extract, 34 parts of Shennong chrysanthemum extract, 5 parts of scutellaria baicalensis extract, 10 parts of sophora japonica extract, 9 parts of milk thistle extract, and 10 parts of sunflower extract.

[0013] Preferably, the plant composition with anti-aging and protective effects further comprises 1-15 parts of butanediol, 0.5-1 part of 1,2-hexanediol, 0.3-0.5 part of p-hydroxyacetophenone and the balance of deionized water based on 100 parts by total weight.

[0014] In a second aspect, the present invention provides the use of the plant composition with anti-aging and protective effects in the preparation of cosmetics with anti-aging and protective effects.

[0015] In a third aspect, the present invention provides a cosmetic having both anti-aging and protective effects, comprising the plant composition having both anti-aging and protective effects.

[0016] Preferably, the plant composition is added in an amount of 10% in the cosmetics having both anti-aging and protective effects.

[0017] Preferably, the cosmetics having both anti-aging and protective effects further include auxiliary materials acceptable in cosmetics.

[0018] Preferably, the dosage forms of the cosmetics with anti-aging and protective effects include water, lotion and cream.

[0019] The beneficial effects of the present invention are:

[0020] The present invention compounds kudzu vine extract, Shennong chrysanthemum extract, scutellaria baicalensis extract, sophora japonica extract, milk thistle extract and sunflower flower extract. The components cooperate with each other to solve the damage of photoaging to the skin from multiple ways such as resisting ultraviolet rays and protecting from sun exposure.

[0021] In the plant composition of the present invention, the plant extract used as the skin care raw material is a natural plant raw material with a fresh smell, safe to the human body, and a simple preparation process.

[0022] The plant composition of the present invention is used to prepare sunscreen and anti-photoaging products, has the characteristics of being natural and safe, and also has the effects of protecting during sun exposure, soothing after sun exposure, resisting ultraviolet rays, and anti-aging. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 The UV absorption spectra of the samples in Test Example 6 are shown in FIG. DETAILED DESCRIPTION

[0024] In order to better illustrate the purpose, technical solutions and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0025] The reagents and raw materials used in the present invention are all commercially available products.

[0026] In the following examples and comparative examples, some components are used from the following sources:

[0027] The kudzu vine extract, Shennong chrysanthemum extract, sophora japonica extract, scutellaria baicalensis extract, sunflower extract and milk thistle extract used in the present invention are all purchased from Natural (Guangzhou) New Material Research and Development Co., Ltd.

[0028] Embodiment 1:

[0029] An embodiment of the plant composition with anti-aging and protective effects of the present invention; the plant composition with anti-aging and protective effects comprises the following components in parts by weight: 12 parts of kudzu vine extract, 22 parts of Shennong chrysanthemum extract, 10 parts of scutellaria baicalensis extract, 11 parts of sophora japonica extract, 13 parts of milk thistle extract, 10 parts of sunflower extract, 15 parts of butylene glycol, 1 part of 1,2-hexanediol, 0.3 parts of p-hydroxyacetophenone and 5.7 parts of deionized water.

[0030] The preparation method of the plant composition with anti-aging and protective effects is: mixing the components evenly to obtain the plant composition with anti-aging and protective effects.

[0031] Embodiment 2:

[0032] An embodiment of the plant composition with anti-aging and protective effects of the present invention; the plant composition with anti-aging and protective effects comprises the following components in parts by weight: 11 parts of kudzu vine extract, 15 parts of Shennong chrysanthemum extract, 8 parts of scutellaria baicalensis extract, 11 parts of sophora japonica extract, 11 parts of milk thistle extract, 8 parts of sunflower extract, 10 parts of butylene glycol, 1 part of 1,2-hexanediol, 0.3 parts of p-hydroxyacetophenone and 24.7 parts of deionized water.

[0033] The preparation method of the plant composition with anti-aging and protective effects is the same as that in Example 1.

[0034] Embodiment 3:

[0035] An embodiment of the plant composition with anti-aging and protective effects of the present invention; the plant composition with anti-aging and protective effects comprises the following components in parts by weight: 9 parts of kudzu vine extract, 34 parts of Shennong chrysanthemum extract, 5 parts of scutellaria baicalensis extract, 10 parts of sophora japonica extract, 9 parts of milk thistle extract, 10 parts of sunflower extract, 10 parts of butylene glycol, 1 part of 1,2-hexanediol, 0.3 parts of p-hydroxyacetophenone and 11.7 parts of deionized water.

[0036] The preparation method of the plant composition with anti-aging and protective effects is the same as that in Example 1.

[0037] Embodiment 4:

[0038] An embodiment of the plant composition with anti-aging and protective effects of the present invention; the plant composition with anti-aging and protective effects comprises the following components in parts by weight: 5 parts of kudzu vine extract, 15 parts of Shennong chrysanthemum extract, 1 part of scutellaria baicalensis extract, 5 parts of sophora japonica extract, 5 parts of milk thistle extract, 1 part of sunflower extract, 1 part of butylene glycol, 0.5 parts of 1,2-hexanediol, 0.3 parts of p-hydroxyacetophenone and 66.2 parts of deionized water.

[0039] The preparation method of the plant composition with anti-aging and protective effects is the same as that in Example 1.

[0040] Comparative Examples 1-8:

[0041] Comparative Examples of the plant composition with anti-aging and protective effects of the present invention; the compositions of the plant composition with anti-aging and protective effects described in Comparative Examples 1-8 are shown in Table 1.

[0042] Table 1 Composition of plant compositions of Comparative Examples 1-8

[0043]

[0044] The preparation method of the plant composition with anti-aging and protective effects is the same as that in Example 1.

[0045] Application Example 1:

[0046] An application example of the cosmetics with anti-aging and protective effects of the present invention; this application example is specifically a protective product, including the plant composition prepared in Example 1; the addition amount of the plant composition in the protective product is 10%.

[0047] The formula of the protective product is shown in Table 2.

[0048] Table 2 Protection product formula

[0049]

[0050]

[0051] The preparation method of the protective product comprises the following steps:

[0052] (1) Mix the components of phase A uniformly, heat to 80-85° C., and homogenize for 1-3 minutes to obtain mixture A;

[0053] (2) Mix the components of phase B evenly, heat to 80° C., and completely dissolve them to obtain a mixture B;

[0054] (3) Add mixture B to mixture A, homogenize for 3 to 5 minutes, and keep warm for 20 minutes;

[0055] (4) Start cooling down. When the temperature drops to 40° C., add the components of phase C in sequence, stir evenly, and then cool down to 35-38° C. to obtain the protective product.

[0056] Application Comparative Example 1:

[0057] An application comparison example of the cosmetics with anti-aging and protective effects described in the present invention; the difference between this application comparison example and the protective product described in Application Example 1 is only that the plant composition prepared in Example 1 in the formula of Application Example 1 is replaced by the plant composition prepared in Comparative Example 2.

[0058] The preparation method of the protective product is the same as that of Application Example 1.

[0059] Application Comparative Example 2:

[0060] An application comparison example of the cosmetics with anti-aging and protective effects described in the present invention; the difference between this application comparison example and the protective product described in Application Example 1 is only that the plant composition prepared in Example 1 in the formula of Application Example 1 is replaced by the plant composition prepared in Comparative Example 8.

[0061] The preparation method of the protective product is the same as that of Application Example 1.

[0062] Test Example 1: Hyaluronidase inhibition test

[0063] The hyaluronidase activity inhibition test was used to verify the anti-allergic and soothing effect of the plant composition. When the allergen invades the body for the first time, the body produces the antibody IgE. When the allergen invades the body again, it combines with the IgE antibody on the surface of the sensitized cell to cause FceRI cross-linking. This starts the activation signal, triggers signal transduction, and activates multiple enzymes including intracellular hyaluronidase. This process is called an allergic reaction. Hyaluronic acid is a component in the tissue matrix that limits the diffusion of water and other extracellular substances. After being hydrolyzed by hyaluronidase, the cells become non-viscous, causing cell degranulation and exudation of new media synthesized, exerting biological effects, and leading to the occurrence of immediate allergic reactions. Hyaluronidase is a participant in allergic reactions. Studies have shown that hyaluronidase is closely related to inflammation and allergies. Inhibiting the activity of hyaluronidase can prevent hyaluronic acid from being decomposed, maintain normal physiological functions, and thus achieve anti-allergic effects. The higher the hyaluronidase activity inhibition rate, the stronger the anti-allergic effect of the substance, and vice versa. Therefore, the inhibition of hyaluronidase activity can be used as an indicator of the anti-inflammatory and anti-allergic response of the sample.

[0064] The test method is as follows:

[0065] Test samples: The plant compositions prepared in Examples 1-4 and Comparative Examples 1-8 were respectively prepared into aqueous solutions with a concentration of 10% (w / w).

[0066] Experimental steps: Take 0.5mL of the test sample and 0.5mL of hyaluronidase solution, and incubate at 37℃ for 20min; add 0.1mL of 2.5mol / L CaCl2, and incubate at 37℃ for 20min; add 0.5mL of sodium hyaluronate solution and keep it at 37℃ for 40min; place it at room temperature for 10min, add 0.5mL of distilled water, 0.1mL of 5mol / L NaOH solution, add 0.5mL of acetylacetone solution and place it in a boiling water bath for 15min, then immediately cool it with ice water for 10min, place it at room temperature for 10min, add 1.0mL of Lehrlich reagent, and dilute it with 4.3mL of anhydrous ethanol, place it at room temperature for 30min to develop color, measure the absorbance at 530nm, and calculate the hyaluronidase inhibition rate according to formula (1). The test results are shown in Table 3.

[0067] Hyaluronidase inhibition rate (%) = [(AB) - (CD)] / (AB) * 100% - Formula (1)

[0068] In the above formula: A is the absorbance of the control solution (using acetate buffer instead of sample solution);

[0069] B is the absorbance of the control blank solution (using acetate buffer instead of sample solution and enzyme solution);

[0070] C is the absorbance of the sample solution;

[0071] D is the absorbance of the blank solution of the sample (using acetate buffer instead of enzyme solution).

[0072] Table 3 Hyaluronidase inhibition effect

[0073]

[0074]

[0075] As can be seen from the above table, the results of hyaluronidase inhibition rate of Examples 1-4 and Control Groups 1-8 are compared. The plant composition obtained by compounding the extract of Pueraria lobata, Shennong Xiangju extract, Scutellaria baicalensis extract, Sophora japonica extract, Milk thistle extract and Sunflower extract in Examples 1-4 of the present invention has a good inhibitory effect on hyaluronidase, and the hyaluronidase inhibition rate significantly reaches more than 40%, which is conducive to the soothing of the skin after sun exposure. The hyaluronidase inhibition rates of the plant compositions prepared in Comparative Examples 3-8 are all lower than those in Examples 1-4, indicating that the lack of any one of the extract of Pueraria lobata, Shennong Xiangju extract, Scutellaria baicalensis extract, Sophora japonica extract, Milk thistle extract and Sunflower extract in the plant composition will affect the soothing effect of the composition. The plant compositions prepared in Comparative Examples 1 and 2, the number of each component exceeds the scope of the present invention, and the hyaluronidase inhibition rate thereof is also lower than that in Examples 1-4.

[0076] In summary, when the skin is exposed to excessive UVB radiation, skin cells will be damaged and inflammation will occur. The plant composition prepared by the present invention can effectively relieve the discomfort caused by inflammation and achieve a soothing effect during and after sun exposure.

[0077] Test Example 2: DPPH free radical scavenging test

[0078] When the skin is exposed to ultraviolet radiation, excessive reactive oxygen species will be generated in the cells, affecting the expression of related genes such as collagen, inducing cell damage and excessive melanin production, resulting in skin spots, sagging, etc. In order to explore the antioxidant effect of the plant composition of the present invention, this test example conducted a DPPH free radical scavenging experiment.

[0079] The test method is as follows:

[0080] Test sample: Same as test example 1.

[0081] Experimental steps: Add 2mL of DPPH free radical solution to a 5mL test tube, then add 600μL of the sample solution to be tested, and then dilute to the 3mL mark with anhydrous ethanol and shake well. After incubating at 25℃ in the dark for 30min, immediately wet the 1cm cuvette with the test solution and measure the absorbance of the reaction solution at 517nm. Repeat the experiment three times and take the average value as the final result. Calculate the DPPH free radical scavenging rate according to formula (2). The test results are shown in Table 4.

[0082] DPPH free radical scavenging rate (%) = Ac-(Ai-Aj) / Ac*100% - Formula (2)

[0083] In the above formula: Ai is the absorbance value of the sample solution + 2 mL DPPH solution; Aj is the absorbance value of the sample solution + 2 mL anhydrous ethanol; Ac is the absorbance value of anhydrous ethanol + 2 mL DPPH solution.

[0084] Table 4 Antioxidant effect

[0085] Test samples Test concentration DPPH free radical scavenging rate (%) Example 1 10％ 64.92 Example 2 10％ 65.15 Example 3 10％ 77.20 Example 4 10％ 60.92 Comparative Example 1 10％ 47.76 Comparative Example 2 10％ 42.76 Comparative Example 3 10％ 37.4 Comparative Example 4 10％ 31.48 Comparative Example 5 10％ 39.58 Comparative Example 6 10％ 45.35 Comparative Example 7 10％ 43.81 Comparative Example 8 10％ 42.53

[0086] As can be seen from the above table, the plant compositions obtained by compounding the extract of Puerariae lobata, the extract of Shennong Xiangju, the extract of Scutellaria baicalensis, the extract of Sophora japonica, the extract of Silybum marianum and the extract of Helianthus annuus in Examples 1-4 of the present invention have a good effect of scavenging DPPH free radicals. Compared with Examples 1-4, the addition amount of some extracts in Comparative Examples 1-2 exceeds or is lower than the range specified in the present invention, and the DPPH free radical scavenging effect is weaker than that of the Examples.

[0087] The DPPH radical scavenging rates of the plant compositions prepared in Comparative Examples 3-8 were all lower than those in Examples 1-4, indicating that the lack of any one of the kudzu vine extract, chrysanthemum extract, scutellaria baicalensis extract, sophora japonica extract, milk thistle extract, and sunflower extract in the plant compositions would affect the DPPH radical scavenging effect of the compositions.

[0088] Therefore, after the present invention compounded the kudzu vine extract, Shennong chrysanthemum extract, scutellaria baicalensis extract, sophora japonica extract, milk thistle extract and sunflower flower extract, the components synergize with each other and are more conducive to scavenging DPPH free radicals, thereby reducing the free radicals generated by the skin after sun exposure and achieving the effect of sun protection.

[0089] Test Example 3: ABTS free radical scavenging test

[0090] When the skin is exposed to ultraviolet radiation, excessive reactive oxygen species will be generated in the cells, affecting the expression of related genes such as collagen, inducing cell damage and excessive melanin production, resulting in skin spots, sagging and other phenomena. In order to explore the antioxidant effect of the plant composition of the present invention, this test example conducted an ABTS free radical scavenging experiment. ABTS is oxidized into green ABTS free radicals under the action of appropriate oxidants. In the presence of antioxidants, the generation of ABTS free radicals will be inhibited. The absorbance value of ABTS free radicals at 405nm can reflect the inhibitory ability of antioxidants.

[0091] The test method is as follows:

[0092] Test sample: Same as test example 1.

[0093] Experimental steps: Add 3.6mL ABTS solution to a 5mL test tube, then add 0.4mL of the test solution, shake well, incubate at room temperature in the dark for 5 minutes, immediately wet the 1cm cuvette with the test solution, and measure the absorbance of the reaction solution at 734nm. Repeat the test three times, and take the average value as the final result. Calculate the ABTS free radical scavenging rate according to formula (3). The test results are shown in Table 5.

[0094] ABTS free radical scavenging rate P (%) = (As-(Ab-A0)) / As*100% - Formula (3)

[0095] In the above formula: P is the clearance rate; As is the absorbance of the mixture of ABTS solution and sample solvent solution; Ab is the absorbance value of the mixture of the test solution and ABTS solution; A0 is the absorbance value of the sample solution and the sample solvent.

[0096] Table 5 ABTS free radical scavenging effect

[0097] Test samples Test concentration ABTS free radical scavenging rate (%) Example 1 10％ 42.87 Example 2 10％ 42.22 Example 3 10％ 43.5 Example 4 10％ 41.3 Comparative Example 1 10％ 19.08 Comparative Example 2 10％ 16.94 Comparative Example 3 10％ 15.62 Comparative Example 4 10％ 14.78 Comparative Example 5 10％ 15.91 Comparative Example 6 10％ 15.24 Comparative Example 7 10％ 14.62 Comparative Example 8 10％ 14.32

[0098] As can be seen from the above table, the ABTS free radical scavenging rate results of Examples 1-4 and Control Groups 1-8 are compared. The plant composition obtained by compounding Pueraria lobata extract, Shennong Xiangju extract, Scutellaria baicalensis extract, Sophora japonica extract, Milk thistle extract and Helianthus annuus extract in Examples 1-4 of the present invention has a good effect of scavenging ABTS free radicals, and the ABTS free radical scavenging rate can reach up to 43.5%.

[0099] Compared with Examples 1-4, the addition amount of some extracts in Comparative Example 1-2 exceeds or is lower than the specified range of the present invention, and the ABTS free radical scavenging effect is weaker than that in the Examples.

[0100] The ABTS free radical scavenging rates of the plant compositions prepared in Comparative Examples 3-8 are all lower than those of Examples 1-4, indicating that the lack of any one of the kudzu vine extract, Shennong chrysanthemum extract, scutellaria baicalensis extract, sophora japonica extract, milk thistle extract, and sunflower flower extract in the plant compositions will affect the ABTS free radical scavenging effect of the composition.

[0101] Test Example 4: Inhibition of lipid peroxidation

[0102] The body will produce oxygen free radicals through enzyme systems or non-enzymatic systems, which can attack polyunsaturated fatty acids in biological membranes, causing lipid peroxidation and forming lipid peroxidation acids. Lipid peroxidation converts reactive oxygen into active chemicals, which, through a series of reactions in the body, cause inflammatory reactions in the skin.

[0103] In order to explore the antioxidant effect of the plant composition of the present invention, this test example conducted an experiment to inhibit linoleic acid lipid peroxidation. Experimental principle: malondialdehyde (MDA), a product of lipid peroxidation metabolites, is used as an indicator to detect the content of lipid peroxides (LPO) and is measured using the thiobarbituric acid method (TBA method). Aldehydes, the oxidation products of unsaturated fatty acids, can react with thiobarbituric acid (TBA) to generate colored compounds, such as malondialdehyde (MDA) and TBA to generate colored substances with maximum absorption at 530nm.

[0104] The test method is as follows:

[0105] Test sample: Same as test example 1.

[0106] Experimental steps: Prepare a 0.5% linoleic acid solution as a substrate, add 1% of the substrate mass to the sample to be tested, and use the substrate without the sample to be tested as a blank control. Place the above samples in an incubator and culture at a constant temperature of (40℃±1℃), take samples after 5 minutes, and determine the amount of malondialdehyde generated by the TBA method. The smaller the absorbance A, the stronger the inhibitory effect of the sample to be tested on lipid peroxidation.

[0107] Table 6 Linoleic acid lipid peroxidation inhibition effect

[0108]

[0109]

[0110] The test results are shown in Table 6. Comparing the linoleic acid lipid peroxidation inhibition effect of Examples 1-4 with that of Control Groups 1-8, the average absorbance values ​​of Examples 1-4 were lower, about 0.42-0.57, indicating that they had a better inhibitory effect on lipid peroxidation.

[0111] Compared with Examples 1-4, the addition amount of some extracts in Comparative Example 1-2 exceeds or is lower than the specified range of the present invention, and the inhibitory effect on lipid peroxidation is weaker than that in the Examples.

[0112] The inhibitory effect of the plant composition prepared in Comparative Examples 3-8 on lipid peroxidation is weaker than that in the examples, indicating that the plant composition lacks any one of the radix puerariae extract, the radix chrysanthemi extract, the radix scutellariae extract, the radix sophorae flavescentis extract, the silybum marianum extract, and the radix sunflower extract, which will have a significant effect on lipid peroxidation, resulting in a weakened inhibitory effect.

[0113] Therefore, after the present invention compounded the kudzu vine extract, the Shennong chrysanthemum extract, the scutellaria baicalensis extract, the sophora japonica extract, the milk thistle extract and the sunflower flower extract, the components synergize with each other and are more conducive to inhibiting lipid peroxidation, thereby reducing the damage to the skin caused by lipid peroxidation and achieving the effect of sun protection.

[0114] Test Example 5: In vitro test of B16 cell melanin synthesis inhibition experiment

[0115] In order to explore the skin-lightening and whitening effects of the plant composition of the present invention, this test example conducted a B16 cell melanin synthesis inhibition experiment. Test principle: UVB mainly affects skin keratinocytes and melanocytes, which can thicken the stratum corneum cells, induce epidermal hyperplasia, aggravate skin inflammation after sun exposure, and also cause the skin to produce melanin and form melanin deposits. B16 mouse melanoma cells are a commonly used cell model for studying melanogenesis. By measuring the changes in the amount of melanin synthesis after the whitening raw materials act on melanocytes, the inhibitory effect of the raw materials on melanin production is evaluated. This test refers to T / SHRH 027-2019 in vitro test B16 cell melanogenesis inhibition test.

[0116] The test method is as follows:

[0117] Test samples: The plant compositions with anti-aging and protective effects prepared in Examples 1-4 and Comparative Examples 1-8 were respectively prepared into solutions with concentrations of 5%, 3%, 1%, 0.5%, and 0.3% (diluted with PBS).

[0118] Experimental steps:

[0119] ①Choice of safe cell concentration: 1×10 4 / well, the cells were inoculated in a 96-well plate for culture; after drug administration, 200 μl of the prepared test sample solution (the control group was PBS, with a concentration of 100%) was added to each well for co-culture, MTT was detected, and cell viability was calculated.

[0120] ② Determination of melanin production: 1×10 4 The cells were inoculated into 96-well plates and cultured; sample solutions of different concentrations below the safe dose were added, cultured together, and the melanin content of the cells was determined.

[0121] The selection results of cell safety concentration and melanin inhibition results are shown in Tables 7 and 8.

[0122] Table 7 Results of cytotoxicity test of each test group on melanocytes

[0123]

[0124] Table 8 Detection results of melanin content

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[0126]

[0127] The MTT test results are shown in Table 7. When the concentration of the test sample solution was less than 3%, the cell activity was greater than 80%. Therefore, the maximum safe concentration of the test sample solution was determined to be 3%.

[0128] The results of the melanin content test are shown in Table 8. By testing the melanin content of B16 cells, it was found that the aqueous solution of the plant composition prepared in Examples 1-4 inhibited the melanin production of B16 cells in a dose-dependent manner within the concentration range of 0.5%-1%, and the maximum inhibition rate could reach 55.4% at a concentration of 1%.

[0129] Compared with Examples 1-4, in Comparative Example 1-2, the addition amount of some extracts exceeded or was lower than the specified range of the present invention, and when the aqueous solution concentration of the plant composition was 0.5%, it had no obvious inhibitory effect on the melanin production of B16 cells.

[0130] The plant compositions prepared in Comparative Examples 3-8 lack any one of the extract of Puerariae lobatae, the extract of Shennong Xiangju, the extract of Scutellaria baicalensis, the extract of Sophora japonicae, the extract of Milk thistle, and the extract of Helianthus annuus, and their inhibitory effects on the melanin production of B16 cells are significantly poor.

[0131] In summary, when the skin is exposed to ultraviolet rays, the production of melanin in skin cells may be accelerated, and the plant composition prepared by the present invention can effectively inhibit the production of melanin, thereby achieving the effects of after-sun repair and whitening.

[0132] Test Example 6: UV absorption test

[0133] In order to explore the ultraviolet absorption effect of the plant composition of the present invention, this test example performed ultraviolet absorption spectrum scanning.

[0134] The test method is as follows:

[0135] Test samples: The plant compositions prepared in Examples 1-3, Comparative Examples 1-3 and Comparative Example 8 were selected and prepared into 1% aqueous solutions respectively.

[0136] Test steps: Use Shimadzu UV-1780 ultraviolet spectrophotometer, set the wavelength range between 280nm-400nm, scan the above test samples one by one, and the ultraviolet absorption results are as follows: Figure 1 shown.

[0137] The results show that when the concentration of the plant composition prepared in Examples 1-3 is 1%, the absorption effect between 280nm-320nm is the best, while the absorbance values ​​of the plant compositions prepared in Comparative Examples 1-3 and Comparative Example 8 are relatively low, indicating that the plant compositions prepared in the embodiments of the present invention have a better protection effect against UVB.

[0138] Test Example 7: Sunscreen Effect Test

[0139] In order to explore the sun protection effect of the plant composition of the present invention, this test example further applies the plant composition of the present invention to cosmetics, and uses a testing instrument to test its SPF value.

[0140] Test samples: protective products prepared using Example 1, Comparative Example 1, and Comparative Example 2, and the final SPF value test results are shown in Table 9.

[0141] Table 9 SPF value test results

[0142] Testing products SPF value test results Application Example 1 2.54 Application Comparative Example 1 1.03 Application Comparative Example 2 1.01

[0143] As shown in Table 9, the compositions prepared in Example 3, Comparative Example 2, and Comparative Example 8 are applied to protective products to obtain protective products prepared in Application Example 1, Application Comparative Example 1, and Application Comparative Example 2, respectively. The SPF value test results show that the SPF value of the protective product of Application Example 1 can reach 2.54, while the number of some components in the plant composition in Application Comparative Example 1 exceeds the scope of the present invention, and its SPF value is significantly reduced by half. The plant composition in Application Comparative Example 2 lacks the sunflower extract, and its SPF value is also significantly lower than that of Application Example 1, indicating that the lack of any component in the plant composition has an impact on the sunscreen value.

[0144] In summary, the plant composition prepared by the present invention has a certain sun protection value when added to cosmetics, and can play a certain role in protecting against sunburn. If it is combined with other sunscreens, the effect may be better.

[0145] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention can be modified or replaced by equivalents without departing from the essence and scope of the technical solution of the present invention.

Claims

1. A plant composition with anti-aging and protective effects, characterized in that: Based on a total weight of 100 parts, the invention comprises the following components in weight parts: 5-12 parts of kudzu vine extract, 15-34 parts of Shennong chrysanthemum extract, 1-10 parts of scutellaria baicalensis extract, 5-11 parts of sophora japonica extract, 5-13 parts of milk thistle extract, 1-10 parts of sunflower extract, 1-15 parts of butylene glycol, 0.5-1 parts of 1,2-hexanediol, 0.3-0.5 parts of p-hydroxyacetophenone and the balance of deionized water.

2. The plant composition with anti-aging and protective effects as claimed in claim 1, characterized in that: Based on 100 parts by total weight, the invention comprises the following components in parts by weight: 9-12 parts of kudzu vine extract, 15-34 parts of Shennong chrysanthemum extract, 5-10 parts of scutellaria baicalensis extract, 10-11 parts of sophora japonica extract, 9-13 parts of milk thistle extract, 8-10 parts of sunflower extract, 1-15 parts of butylene glycol, 0.5-1 parts of 1,2-hexanediol, 0.3-0.5 parts of p-hydroxyacetophenone and the balance of deionized water.

3. The plant composition with anti-aging and protective effects as claimed in claim 2, characterized in that: Based on a total weight of 100 parts, the invention comprises the following components in weight: 9 parts of kudzu vine extract, 34 parts of chrysanthemum extract, 5 parts of scutellaria baicalensis extract, 10 parts of sophora japonica seed extract, 9 parts of milk thistle extract, 10 parts of sunflower flower extract, 1-15 parts of butylene glycol, 0.5-1 parts of 1,2-hexanediol, 0.3-0.5 parts of p-hydroxyacetophenone and the balance of deionized water.

4. Use of the plant composition with anti-aging and protective effects as claimed in any one of claims 1 to 3 in the preparation of cosmetics with anti-aging and protective effects.

5. A cosmetic with anti-aging and protective effects, characterized in that: The invention comprises the plant composition with anti-aging and protective effects as described in any one of claims 1 to 3.

6. The cosmetic with anti-aging and protective effects according to claim 5, characterized in that: The added amount of the plant composition in the cosmetics with anti-aging and protective effects is 10%.

7. The cosmetic with anti-aging and protective effects according to claim 5, characterized in that: The cosmetics with anti-aging and protective effects also include auxiliary materials acceptable in cosmetics.

8. The cosmetic with anti-aging and protective effects according to claim 5, characterized in that: The dosage forms of the cosmetics with anti-aging and protective effects include water, milk and cream.

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