Preparation and application of Shenxianling granules, a traditional Chinese medicine preparation for treating digestive tract tumors

Abstract

The present invention discloses a preparation method of Shenxianling granules, a traditional Chinese medicine preparation for treating digestive tract tumors, and its application, which relates to the technical field of traditional Chinese medicines, and comprises the following steps: (1) preparing materials; (2) dividing materials; (3) mixing materials; and (4) granulating. The active ingredients in the Shenxianling granules, a traditional Chinese medicine preparation prepared by the present invention, can directly or indirectly inhibit digestive tract tumor cells, and at the same time regulate the immune function of the body, inhibit the growth of digestive tract tumor cells, and have a good inhibitory effect.

Description

Technical Field

[0001] The invention belongs to the technical field of traditional Chinese medicines, and in particular to the preparation and application of Shenxianling granules, a traditional Chinese medicine preparation for treating digestive tract tumors. Background Art

[0002] Digestive tract tumors are a broad concept that covers both benign and malignant tumors in the digestive tract. They include tumor lesions that may occur in the entire digestive tract from the mouth to the anus. These tumors may occur in any part of the digestive tract, such as the esophagus, stomach, small intestine, and large intestine. There are many types of digestive tract tumors, among which the most common malignant tumors include gastric cancer, esophageal cancer, rectal cancer, and colon cancer, while benign tumors include neurofibromas, hemangiomas, stromal tumors, and adenomas.

[0003] The symptoms of digestive tract tumors vary depending on the location, size, and degree of benign or malignant tumors. In the early stages of a tumor, the patient may have no obvious symptoms or only some mild discomfort because the tumor is small and has not invaded surrounding tissues. However, as the tumor grows and spreads, the symptoms will gradually appear and worsen. For example, in the late stages of a malignant tumor of the stomach, symptoms of nausea, vomiting, and vomiting blood may occur, and intestinal tumors may cause bloody stools or black tarry stools.

[0004] In the treatment of digestive tract tumors, Chinese medicine, as a traditional treatment method, is gradually gaining more and more attention and recognition. With its unique pharmacological effects and comprehensive conditioning characteristics, Chinese medicine provides a new treatment option for patients with digestive tract tumors.

[0005] Based on this, a preparation method and application of Shenxianling granules, a traditional Chinese medicine preparation for treating digestive tract tumors, is provided to solve existing technical problems. Summary of the invention

[0006] The purpose of the present invention is to provide a preparation method and application of Shenxianling granules, a traditional Chinese medicine preparation for treating digestive tract tumors, so as to solve the deficiencies in the prior art.

[0007] The technical solution adopted by the present invention is as follows:

[0008] A preparation method of Shenxianling granules, a traditional Chinese medicine preparation for treating digestive tract tumors, comprises the following steps:

[0009] (1) Preparation of materials: the following components are included by weight: 30-35 parts of Astragalus, 12-16 parts of Ceropegia ciliata, 3-5 parts of Pinellia ternata, 1-3 parts of Hedyotis diffusa, 0.2-0.5 parts of Rehmannia glutinosa, and 0.1-0.3 parts of Angelica sinensis;

[0010] (2) Material separation: Astragalus, Cyperus rotundus, Pinellia ternata, and Hedyotis diffusa are processed to obtain material 1;

[0011] Processing Rehmannia glutinosa and Chinese Angelica sinensis to obtain material 2;

[0012] (3) Mixing: Add material 1 and material 2, dextrin, starch and water into a blender in sequence, stir and mix to obtain a slurry;

[0013] (4) Granulation: Add the slurry into the side spray pot of the fluidized bed, control the bed temperature at 40-45°C, make spherical particles, and dry them to obtain the product.

[0014] As a further technical solution: the preparation method of material 1 is:

[0015] (1) First, astragalus, cyperus rotundus, pinellia, and oldenlandia diffusa were mixed together, and then 10 times the total mass of water was added, and the mixture was heated to 100°C at a heating rate of 10°C / min, and the mixture was stirred for 10 minutes, and then the temperature was adjusted to 80°C and kept in a water bath for 2 hours to obtain a mixed decoction;

[0016] Among them, the stirring speed is 100r / min;

[0017] (2) adding 50% of the total weight of anhydrous ethanol to the mixed decoction, performing reflux extraction for 4 hours, and then performing high-speed centrifugation for 15 minutes, and then filtering to remove the filter residue to obtain a filtrate;

[0018] (3) The filtrate is decompressed to recover ethanol, and then spray-dried to obtain material 1.

[0019] As a further technical solution: the centrifugal speed of the high-speed centrifugal treatment is 5000r / min.

[0020] As a further technical solution: the preparation method of material 2 is:

[0021] First, soak the Radix Rehmanniae and Radix Angelicae Sinensis in sodium bicarbonate solution for 10 minutes, then take them out, wash them with clean water until they are neutral, and then dry them;

[0022] The soaked Rehmannia glutinosa is subjected to steam fumigation for 20 minutes, cooled to room temperature, and then vacuum dried and crushed to obtain Rehmannia glutinosa powder;

[0023] The angelica sinensis was subjected to microwave treatment for 10 minutes, and then crushed to obtain angelica sinensis powder;

[0024] Add the Rehmannia glutinosa powder and Angelica sinensis powder into a blender, and stir to mix evenly to obtain material 2;

[0025] Among them, the stirring speed is 150r / min.

[0026] As a further technical solution: the mass fraction of the sodium bicarbonate solution is 10%;

[0027] The total mass ratio of the sodium bicarbonate solution to the Radix Rehmanniae Preparata and the Radix Angelicae Sinensis is 200 mL: 10-14 g;

[0028] Among them, the soaking temperature is 35℃.

[0029] As a further technical solution: the water vapor fumigation temperature is 102°C.

[0030] As a further technical solution: the angelica powder has a particle size of 100 mesh;

[0031] The particle size of the Rehmannia glutinosa powder is 150 meshes.

[0032] As a further technical solution: in step (3), the stirring speed of the stirrer is 2000 r / min and the stirring time is 2 hours.

[0033] As a further technical solution: the spherical particles in step (4) have a bulk density of 0.802-0.850 g / ml.

[0034] A traditional Chinese medicine preparation for treating digestive tract tumors, Shenxianling Granules, is used to treat rectal tumors.

[0035] Beneficial effects:

[0036] The active ingredients in the traditional Chinese medicine preparation Shenxianling granules prepared by the invention can directly or indirectly inhibit digestive tract tumor cells, and at the same time regulate the body's immune function to inhibit the growth of digestive tract tumor cells, with good inhibitory effect.

[0037] The ingredients in the traditional Chinese medicine preparation Shenxianling granules prepared by the present invention have antibacterial and anti-inflammatory effects, can reduce the inflammatory response caused by digestive tract tumors, thereby quickly alleviating the patient's inflammatory symptoms and improving the anti-inflammatory process. At the same time, it can improve blood circulation, promote the metabolism and repair of tissues around tumors, and help reduce the pressure and damage of tumors on surrounding tissues. Through the coordinated action of various ingredients, the body's immunity can be further enhanced.

[0038] The present invention uses material 1 and material 2 to cooperate with each other to inhibit the proliferation of digestive tract tumor cells, induce cell apoptosis and other ways to play a role in inhibiting tumors.

[0039] The active ingredients extracted from Pinellia ternata and Oldenlandia diffusa can directly attack tumor cells and inhibit their growth and spread.

[0040] Astragalus can warm the spleen and stomach, making the circulation of qi and blood smoother, which is beneficial to eliminating the phenomenon of qi stagnation and blood stasis, thereby reducing the possibility of tumor formation. Therefore, the present invention can help patients alleviate edema symptoms and improve physical condition by introducing astragalus in combination with the effects of other multiple components through diuresis and dehumidification. At the same time, it can also promote the peristalsis of the digestive tract, relieve patients' symptoms such as indigestion, nausea and vomiting, and help improve patients' appetite and quality of life.

[0041] The Chinese medicine preparation Shenxianling granules prepared by the present invention contain more trace elements, can play an anti-oxidation and anti-tumor role, and can also adjust the content of CAMP and CGMP in human cells, and enhance the phagocytic function of the reticuloendothelial system, all of which are beneficial to inhibiting the growth and spread of tumor cells. BRIEF DESCRIPTION OF THE DRAWINGS

[0042] Figure 1 It is a bar graph of the tumor inhibition rate of each sample in the experiment. DETAILED DESCRIPTION

[0043] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0044] The following are specific embodiments:

[0045] Example 1

[0046] A preparation method of Shenxianling granules, a traditional Chinese medicine preparation for treating digestive tract tumors, comprises the following steps:

[0047] (1) Preparation: The following components are included by weight: 30 parts of Astragalus, 12 parts of Ceropegia ciliata, 3 parts of Pinellia ternata, 1 part of Hedyotis diffusa, 0.2 parts of Rehmannia glutinosa, and 0.1 parts of Angelica sinensis;

[0048] (2) Material separation: Astragalus, Cyperus rotundus, Pinellia ternata, and Hedyotis diffusa are processed to obtain material 1;

[0049] Processing Rehmannia glutinosa and Chinese Angelica sinensis to obtain material 2;

[0050] (3) Mixing: Add material 1 and material 2, dextrin, starch and water to a blender in sequence, and stir to obtain a slurry; the stirring speed of the blender is 2000 r / min, and the stirring time is 2 hours.

[0051] (4) Granulation: Add the slurry into the side spray pot of the fluidized bed, control the bed temperature at 40°C, make spherical particles, and dry them to obtain the product.

[0052] The preparation method of material 1 is:

[0053] (1) First, astragalus, cyperus rotundus, pinellia, and oldenlandia diffusa were mixed together, and then 10 times the total mass of water was added, and the mixture was heated to 100°C at a heating rate of 10°C / min, and the mixture was stirred for 10 minutes, and then the temperature was adjusted to 80°C and kept in a water bath for 2 hours to obtain a mixed decoction;

[0054] Among them, the stirring speed is 100r / min;

[0055] (2) adding 50% of the total weight of anhydrous ethanol to the mixed decoction, performing reflux extraction for 4 hours, and then performing high-speed centrifugation for 15 minutes, and then filtering to remove the filter residue to obtain a filtrate;

[0056] (3) The filtrate is decompressed to recover ethanol, and then spray-dried to obtain material 1.

[0057] The centrifugal speed of the high-speed centrifugation treatment is 5000r / min.

[0058] The preparation method of material 2 is:

[0059] First, soak the Radix Rehmanniae and Radix Angelicae Sinensis in sodium bicarbonate solution for 10 minutes respectively, then take them out, wash them with clean water until they are neutral, and then dry them; the mass fraction of the sodium bicarbonate solution is 10%;

[0060] The total mass ratio of the sodium bicarbonate solution to the prepared rehmannia root and the angelica root is 200 mL:10 g; wherein the soaking temperature is 35°C.

[0061] The soaked Rehmannia glutinosa was subjected to steam fumigation for 20 minutes, cooled to room temperature, vacuum dried, and crushed to obtain Rehmannia glutinosa powder; the steam fumigation temperature was 102°C.

[0062] The angelica sinensis was subjected to microwave treatment for 10 minutes, and then crushed to obtain angelica sinensis powder;

[0063] Add the Rehmannia glutinosa powder and Angelica sinensis powder into a blender, and stir to mix evenly to obtain material 2;

[0064] The stirring speed is 150r / min. The particle size of angelica powder is 100 mesh; the particle size of prepared rehmannia powder is 150 mesh.

[0065] Example 2

[0066] A preparation method of Shenxianling granules, a traditional Chinese medicine preparation for treating digestive tract tumors, comprises the following steps:

[0067] (1) Preparation: The following components are included by weight: 32 parts of Astragalus, 13 parts of Ceropegia ciliata, 4 parts of Pinellia ternata, 2 parts of Hedyotis diffusa, 0.3 parts of Rehmannia glutinosa, and 0.2 parts of Angelica sinensis;

[0068] (2) Material separation: Astragalus, Cyperus rotundus, Pinellia ternata, and Hedyotis diffusa are processed to obtain material 1;

[0069] Processing Rehmannia glutinosa and Chinese Angelica sinensis to obtain material 2;

[0070] (3) Mixing: Add material 1 and material 2, dextrin, starch and water to a blender in sequence, and stir to obtain a slurry; the stirring speed of the blender is 2000 r / min, and the stirring time is 2 hours.

[0071] (4) Granulation: Add the slurry into the side spray pot of the fluidized bed, control the bed temperature at 42°C, make spherical particles, and obtain the product after drying.

[0072] The preparation method of material 1 is:

[0073] (1) First, astragalus, cyperus rotundus, pinellia, and oldenlandia diffusa were mixed together, and then 10 times the total mass of water was added, and the mixture was heated to 100°C at a heating rate of 10°C / min, and the mixture was stirred for 10 minutes. Then, the temperature was adjusted to 80°C and kept in a water bath for 2 hours to obtain a mixed decoction;

[0074] Among them, the stirring speed is 100r / min;

[0075] (2) adding 50% of the total weight of anhydrous ethanol to the mixed decoction, performing reflux extraction for 4 hours, and then performing high-speed centrifugation for 15 minutes, and then filtering to remove the filter residue to obtain a filtrate;

[0076] (3) The filtrate is decompressed to recover ethanol, and then spray-dried to obtain material 1.

[0077] The centrifugal speed of the high-speed centrifugal treatment is 5000r / min.

[0078] The preparation method of material 2 is:

[0079] First, soak the Radix Rehmanniae and Radix Angelicae Sinensis in sodium bicarbonate solution for 10 minutes respectively, then take them out, wash them with clean water until they are neutral, and then dry them; the mass fraction of the sodium bicarbonate solution is 10%;

[0080] The total mass ratio of the sodium bicarbonate solution to the prepared rehmannia root and the angelica root is 200 mL:12 g; wherein the soaking temperature is 35°C.

[0081] The soaked Rehmannia glutinosa was subjected to steam fumigation for 20 minutes, cooled to room temperature, vacuum dried, and crushed to obtain Rehmannia glutinosa powder; the steam fumigation temperature was 102°C.

[0082] The angelica sinensis was subjected to microwave treatment for 10 minutes, and then crushed to obtain angelica sinensis powder;

[0083] Add the Rehmannia glutinosa powder and Angelica sinensis powder into a blender, and stir to mix evenly to obtain material 2;

[0084] The stirring speed is 150r / min. The particle size of angelica powder is 100 mesh; the particle size of prepared rehmannia powder is 150 mesh.

[0085] Example 3

[0086] A preparation method of Shenxianling granules, a traditional Chinese medicine preparation for treating digestive tract tumors, comprises the following steps:

[0087] (1) Preparation: The following components are included by weight: 33 parts of Astragalus, 15 parts of Ceropegia ciliata, 3 parts of Pinellia ternata, 2 parts of Hedyotis diffusa, 0.3 parts of Rehmannia glutinosa, and 0.2 parts of Angelica sinensis;

[0088] (2) Material separation: Astragalus, Cyperus rotundus, Pinellia ternata, and Hedyotis diffusa are processed to obtain material 1;

[0089] Processing Rehmannia glutinosa and Chinese Angelica sinensis to obtain material 2;

[0090] (3) Mixing: Add material 1 and material 2, dextrin, starch and water to a blender in sequence, and stir to obtain a slurry; the stirring speed of the blender is 2000 r / min, and the stirring time is 2 hours.

[0091] (4) Granulation: Add the slurry into the side spray pot of the fluidized bed, control the bed temperature at 44°C, make spherical particles, and dry them to obtain the product.

[0092] The preparation method of material 1 is:

[0093] (1) First, astragalus, cyperus rotundus, pinellia, and oldenlandia diffusa were mixed together, and then 10 times the total mass of water was added, and the mixture was heated to 100°C at a heating rate of 10°C / min, and the mixture was stirred for 10 minutes. Then, the temperature was adjusted to 80°C and kept in a water bath for 2 hours to obtain a mixed decoction;

[0094] Among them, the stirring speed is 100r / min;

[0095] (2) adding 50% of the total weight of anhydrous ethanol to the mixed decoction, performing reflux extraction for 4 hours, and then performing high-speed centrifugation for 15 minutes, and then filtering to remove the filter residue to obtain a filtrate;

[0096] (3) The filtrate is decompressed to recover ethanol, and then spray-dried to obtain material 1.

[0097] The centrifugal speed of the high-speed centrifugal treatment is 5000r / min.

[0098] The preparation method of material 2 is:

[0099] First, soak the Radix Rehmanniae and Radix Angelicae Sinensis in sodium bicarbonate solution for 10 minutes respectively, then take them out, wash them with clean water until they are neutral, and then dry them; the mass fraction of the sodium bicarbonate solution is 10%;

[0100] The total mass ratio of the sodium bicarbonate solution to the Rehmannia glutinosa and Angelica sinensis mixed is 200mL:13g; wherein, the soaking temperature is 35°C.

[0101] The soaked Rehmannia glutinosa was subjected to steam fumigation for 20 minutes, cooled to room temperature, vacuum dried, and crushed to obtain Rehmannia glutinosa powder; the steam fumigation temperature was 102°C.

[0102] The angelica sinensis was subjected to microwave treatment for 10 minutes, and then crushed to obtain angelica sinensis powder;

[0103] Add the Rehmannia glutinosa powder and Angelica sinensis powder into a blender, and stir to mix evenly to obtain material 2;

[0104] The stirring speed is 150r / min. The particle size of angelica powder is 100 mesh; the particle size of prepared rehmannia powder is 150 mesh.

[0105] Example 4

[0106] A preparation method of Shenxianling granules, a traditional Chinese medicine preparation for treating digestive tract tumors, comprises the following steps:

[0107] (1) Preparation: The following components are included by weight: 34 parts of Astragalus, 12 parts of Ceropegia ciliata, 3 parts of Pinellia ternata, 3 parts of Hedyotis diffusa, 0.2 parts of Rehmannia glutinosa, and 0.1 parts of Angelica sinensis;

[0108] (2) Material separation: Astragalus, Cyperus rotundus, Pinellia ternata, and Hedyotis diffusa are processed to obtain material 1;

[0109] Processing Rehmannia glutinosa and Chinese Angelica sinensis to obtain material 2;

[0110] (3) Mixing: Add material 1 and material 2, dextrin, starch and water to a blender in sequence, and stir to obtain a slurry; the stirring speed of the blender is 2000 r / min, and the stirring time is 2 hours.

[0111] (4) Granulation: Add the slurry into the side spray pot of the fluidized bed, control the bed temperature at 41°C, make spherical particles, and obtain the product after drying.

[0112] The preparation method of material 1 is:

[0113] (1) First, astragalus, cyperus rotundus, pinellia, and oldenlandia diffusa were mixed together, and then 10 times the total mass of water was added, and the mixture was heated to 100°C at a heating rate of 10°C / min, and the mixture was stirred for 10 minutes. Then, the temperature was adjusted to 80°C and kept in a water bath for 2 hours to obtain a mixed decoction;

[0114] Among them, the stirring speed is 100r / min;

[0115] (2) adding 50% of the total weight of anhydrous ethanol to the mixed decoction, performing reflux extraction for 4 hours, and then performing high-speed centrifugation for 15 minutes, and then filtering to remove the filter residue to obtain a filtrate;

[0116] (3) The filtrate is decompressed to recover ethanol, and then spray-dried to obtain material 1.

[0117] The centrifugal speed of the high-speed centrifugal treatment is 5000r / min.

[0118] The preparation method of material 2 is:

[0119] First, soak the Radix Rehmanniae and Radix Angelicae Sinensis in sodium bicarbonate solution for 10 minutes respectively, then take them out, wash them with clean water until they are neutral, and then dry them; the mass fraction of the sodium bicarbonate solution is 10%;

[0120] The total mass ratio of the sodium bicarbonate solution to the prepared rehmannia root and the angelica root is 200 mL:10 g; wherein the soaking temperature is 35°C.

[0121] The soaked Rehmannia glutinosa was subjected to steam fumigation for 20 minutes, cooled to room temperature, vacuum dried, and crushed to obtain Rehmannia glutinosa powder; the steam fumigation temperature was 102°C.

[0122] The angelica sinensis was subjected to microwave treatment for 10 minutes, and then crushed to obtain angelica sinensis powder;

[0123] Add the Rehmannia glutinosa powder and Angelica sinensis powder into a blender, and stir to mix evenly to obtain material 2;

[0124] The stirring speed is 150r / min. The particle size of angelica powder is 100 mesh; the particle size of prepared rehmannia powder is 150 mesh.

[0125] Example 5

[0126] A preparation method of Shenxianling granules, a traditional Chinese medicine preparation for treating digestive tract tumors, comprises the following steps:

[0127] (1) Preparation: The following components are included by weight: 35 parts of Astragalus, 16 parts of Cephalotaxus glabra, 5 parts of Pinellia, 3 parts of Hedyotis diffusa, 0.5 parts of Rehmannia glutinosa, and 0.3 parts of Angelica sinensis;

[0128] (2) Material separation: Astragalus, Cyperus rotundus, Pinellia ternata, and Hedyotis diffusa are processed to obtain material 1;

[0129] Processing Rehmannia glutinosa and Chinese Angelica sinensis to obtain material 2;

[0130] (3) Mixing: Add material 1 and material 2, dextrin, starch and water to a blender in sequence, and stir to obtain a slurry; the stirring speed of the blender is 2000 r / min, and the stirring time is 2 hours.

[0131] (4) Granulation: Add the slurry into the side spray pot of the fluidized bed, control the bed temperature at 45°C, make spherical particles, and dry them to obtain the product.

[0132] The preparation method of material 1 is:

[0133] (1) First, astragalus, cyperus rotundus, pinellia, and oldenlandia diffusa were mixed together, and then 10 times the total mass of water was added, and the mixture was heated to 100°C at a heating rate of 10°C / min, and the mixture was stirred for 10 minutes, and then the temperature was adjusted to 80°C and kept in a water bath for 2 hours to obtain a mixed decoction;

[0134] Among them, the stirring speed is 100r / min;

[0135] (2) adding 50% of the total weight of anhydrous ethanol to the mixed decoction, performing reflux extraction for 4 hours, and then performing high-speed centrifugation for 15 minutes, and then filtering to remove the filter residue to obtain a filtrate;

[0136] (3) The filtrate is decompressed to recover ethanol, and then spray-dried to obtain material 1.

[0137] The centrifugal speed of the high-speed centrifugation treatment is 5000r / min.

[0138] The preparation method of material 2 is:

[0139] First, soak the Radix Rehmanniae and Radix Angelicae Sinensis in sodium bicarbonate solution for 10 minutes respectively, then take them out, wash them with clean water until they are neutral, and then dry them; the mass fraction of the sodium bicarbonate solution is 10%;

[0140] The total mass ratio of the sodium bicarbonate solution to the prepared rehmannia root and the angelica root is 200 mL:14 g; wherein the soaking temperature is 35°C.

[0141] The soaked Rehmannia glutinosa was subjected to steam fumigation for 20 minutes, cooled to room temperature, vacuum dried, and crushed to obtain Rehmannia glutinosa powder; the steam fumigation temperature was 102°C.

[0142] The angelica sinensis was subjected to microwave treatment for 10 minutes, and then crushed to obtain angelica sinensis powder;

[0143] Add the Rehmannia glutinosa powder and Angelica sinensis powder into a blender, and stir to mix evenly to obtain material 2;

[0144] The stirring speed is 150r / min. The particle size of angelica powder is 100 mesh; the particle size of prepared rehmannia powder is 150 mesh.

[0145] Comparative Example 1:

[0146] The difference from Example 1 is that Rehmannia glutinosa and Angelica sinensis are processed together with the raw materials in Material 1 to obtain Material 2, and Material 2 is used as the raw material to prepare the Chinese medicine preparation Shenxianling Granules, and the rest of the technical scheme remains unchanged.

[0147] Comparative Example 2

[0148] The difference from Example 1 is that cooked rehmannia is not added to the raw material components, and the rest of the technical solutions remain unchanged.

[0149] Comparative Example 3

[0150] The difference from Example 1 is that Angelica sinensis is not added to the raw material components, and the rest of the technical solutions remain unchanged.

[0151] test:

[0152] After one week of adaptive feeding, the experimental mice (half male and half female, 20 g) were observed to be in good vitality and then randomly divided into 9 groups. Tumor cells were subcutaneously inoculated to form tumors, and a subcutaneous colorectal tumor model of experimental mice was established. The weight of the mice was recorded once a day.

[0153] Administration began 24 hours after inoculation. The Example and the Comparative Example were diluted with water at a ratio of 1:5, and 8 groups were gavaged once a day for 34 consecutive days (dosage was 20 mL / kg) as the test group, and one group was given an equal amount of normal saline as the control group:

[0154] Tumor inhibition rate (%) = (1-average tumor weight of the experimental group / average tumor weight of the control group) × 100%;

[0155] Table 1

[0156] Tumor inhibition rate% Example 1 65.3 Example 2 66.0 Example 3 65.8 Example 4 64.2 Example 5 64.6 Comparative Example 1 49.7 Comparative Example 2 58.0 Comparative Example 3 55.3

[0157] It can be seen from Table 1 that the Chinese medicine preparation Shenxianling Granules prepared by the present invention has a strong tumor inhibitory effect.

[0158] The tumors of the mice in the above groups were taken and weighed for comparison:

[0159] Table 2

[0160]

[0161]

[0162] It can be seen from Table 2 that the Chinese medicine preparation Shenxianling Granules prepared by the present invention can inhibit the continued growth of tumors.

[0163] The above description is only a preferred embodiment of the present invention, but the present invention is not limited to the scope of implementation shown. Any changes made in accordance with the concept of the present invention, or modifications to equivalent embodiments with equivalent changes, which still do not exceed the spirit covered by the specification, should be within the protection scope of the present invention.

Claims

1. A method for preparing Shenxianling granules, a traditional Chinese medicine preparation for treating digestive tract tumors, characterized in that: The following steps are involved: (1) Preparation: The following components are included by weight: 30-35 parts of Astragalus, 12-16 parts of Cyperus rotundus, 3-5 parts of Pinellia, 1-3 parts of Hedyotis diffusa, 0.2-0.5 parts of Rehmannia glutinosa, and 0.1-0.3 parts of Angelica sinensis; (2) Material separation: Astragalus, Cyperus rotundus, Pinellia ternata, and Hedyotis diffusa are processed to obtain material 1; Processing Rehmannia glutinosa and Chinese Angelica sinensis to obtain material 2; (3) Mixing: Add material 1 and material 2, dextrin, starch and water into a mixer in sequence, stir and mix to obtain a slurry; (4) Granulation: Add the slurry into the side spray pot of the fluidized bed, control the bed temperature at 40-45°C, make spherical particles, and dry them to obtain; The preparation method of material 1 is: (1) First, mix Astragalus, Cyperus rotundus, Pinellia ternata, and Hedyotis diffusa together, then add 10 times the total mass of water after the mixture, heat to 100°C at a heating rate of 10°C / min, keep warm and stir for 10 min, then adjust the temperature to 80°C, keep warm in a water bath for 2 hours, and obtain a mixed decoction; Among them, the stirring speed is 100r / min; (2) adding 50% of the total weight of anhydrous ethanol to the mixed decoction, extracting by reflux for 4 hours, and then subjecting to high-speed centrifugation for 15 minutes, filtering and removing the filter residue to obtain a filtrate; (3) Recovering ethanol from the filtrate under reduced pressure, and then spray drying to obtain material 1; The centrifugal speed of the high-speed centrifugation is 5000 r / min; The preparation method of material 2 is: First, soak the Radix Rehmanniae and Radix Angelicae Sinensis in sodium bicarbonate solution for 10 minutes, then take them out, wash them with clean water until they are neutral, and then dry them; The soaked Rehmannia glutinosa is subjected to steam fumigation for 20 minutes, cooled to room temperature, and then vacuum dried and crushed to obtain Rehmannia glutinosa powder; The angelica sinensis was subjected to microwave treatment for 10 minutes, and then crushed to obtain angelica sinensis powder; Add the Rehmannia glutinosa powder and Angelica sinensis powder into a blender, and stir to mix evenly to obtain material 2; Among them, the stirring speed is 150r / min.

2. The method for preparing the traditional Chinese medicine preparation Shenxianling granules for treating digestive tract tumors according to claim 1, characterized in that: The mass fraction of the sodium bicarbonate solution is 10%; The total mass ratio of the sodium bicarbonate solution to the Radix Rehmanniae Preparata and the Radix Angelicae Sinensis is 200mL:10-14g; Among them, the soaking temperature is 35°C.

3. The method for preparing the traditional Chinese medicine preparation Shenxianling granules for treating digestive tract tumors according to claim 1, characterized in that: The water vapor fumigation temperature is 102°C.

4. The method for preparing the traditional Chinese medicine preparation Shenxianling granules for treating digestive tract tumors according to claim 1, characterized in that: The angelica powder has a particle size of 100 mesh; The particle size of the Rehmannia glutinosa powder is 150 meshes.

5. The method for preparing the traditional Chinese medicine preparation Shenxianling granules for treating digestive tract tumors according to claim 1, characterized in that: In step (3), the stirring speed of the stirrer is 2000 r / min and the stirring time is 2 hours.

6. The method for preparing the traditional Chinese medicine preparation Shenxianling granules for treating digestive tract tumors according to claim 1, characterized in that: The spherical particles in step (4) have a bulk density of 0.802-0.850 g / ml.

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