A self-driving micro-hybrid chip

Abstract

The present invention discloses a self-driven micro-mixing chip, including a main passage, the side of which is connected with storage unit A, storage unit B and storage unit C in sequence from left to right; the tail end of the main passage is connected with arc cavity A, arc cavity B, arc cavity C and circular cavity in sequence from one side of the input port to the side of the outlet. The present invention provides multiple storage units, main channels and capillary pumps; multiple reagents can be discharged in sequence, mixed with blood samples in the main passage, and transported to the mixing channel after concentration. The mixing channel mainly forms a multi-dimensional secondary flow effect through the circular arc and semicircle formed inside it, thereby increasing the contact area between multiple reagents and blood samples, while increasing the overall mixing performance of the structure, further improving its practicality and the scope of application of the structure, and at the same time, improving the utilization rate of the microfluidic chip.

Description

Technical Field

[0001] The present invention relates to the technical field of solution mixing, and more specifically, to a self-driven micro-mixing chip. Background Art

[0002] Cortisol testing procedures: 1. Blood test: The most common method for cortisol testing is a simple blood test. A healthcare professional will draw a small amount of blood from a vein, usually in the arm, and the blood sample is then sent to a laboratory for analysis. It is important to follow any specific instructions given by the healthcare provider, such as fasting before the test or testing in the morning when cortisol levels are typically higher; 2. Saliva test: Another method for cortisol testing is a saliva test. This non-invasive procedure involves collecting a saliva sample at a specific time of the day. The saliva cortisol test is convenient and can be done at home, making it an attractive option for long-term monitoring; 3. Urine test: In some cases, a 24-hour urine test may be recommended to measure cortisol levels. The test involves collecting all urine produced within 24 hours and submitting it for analysis. It provides a comprehensive view of cortisol production throughout the day. Among them, blood testing is the most common and accurate testing procedure. When testing a blood sample, it is usually necessary to automatically mix the reagent (solution) with the blood in a micromixer through a capillary tube to achieve a finger-prick blood-based assay.

[0003] However, there are still some shortcomings in its actual use. For example, the flow of the microfluidic mixing devices currently used in the cortisol detection program usually has a small Reynolds number, which means that most of the flow is laminar and turbulent, and two or more liquids cannot be mixed by convection, resulting in the inability to quickly and thoroughly mix the solutions. In addition, most of the mixers currently used require continuous liquid supply and a stable driving source, which has certain restrictions on POCT devices that require mixing, resulting in a long measurement process, high operating costs, and is limited to laboratory testing and cannot be expanded on a large scale. Summary of the invention

[0004] In order to overcome the above-mentioned defects of the prior art, an embodiment of the present invention provides a self-driven micro-mixing chip, which is equipped with multiple storage units, a main channel and a capillary pump; multiple reagents can be discharged in sequence, mixed with the blood sample in the main channel, and transported to the mixing channel after concentration. The mixing channel mainly forms a multi-dimensional secondary flow effect through the arc shape and semicircle formed inside it, thereby increasing the contact area between multiple reagents and the blood sample, and the suddenly increased channel cross-sectional area can further shorten the length between different reagents to avoid stratification between reagents. While increasing the overall mixing performance of the structure, the utilization rate of the microfluidic chip can be improved to solve the problems raised in the above-mentioned background technology.

[0005] To achieve the above object, the present invention provides the following technical solution: a self-driven micro-hybrid chip, comprising a main passage, wherein both ends of the main passage are respectively provided with an input port and an outlet, one end of the outlet is connected to a capillary pump, and the side of the main passage is connected to storage unit A, storage unit B and storage unit C in sequence from left to right;

[0006] The tail end of the main passage is connected with arc cavity A, arc cavity B, arc cavity C and circular cavity in sequence from one side of the input port to the other side of the outlet port, the axial angles between the arc cavity A, arc cavity B, arc cavity C and circular cavity are vertically distributed, and the arc cavity A and the circular cavity are located on the same plane, the surfaces of the main passage between the arc cavity B and the arc cavity C are oppositely distributed, the arc cavity A and the arc cavity B have overlapping parts in the horizontal position direction, the arc cavity B and the arc cavity C are close to each other on the same horizontal plane, and the arc cavity C and the circular cavity have overlapping parts in the horizontal position direction;

[0007] The storage unit A comprises a liquid storage cavity, an inlet is provided at one side of the top of the liquid storage cavity, a trigger valve is provided at the bottom of the liquid storage cavity, a cavity is provided on the side of the liquid storage cavity, a burst valve is provided between the cavity and the liquid storage cavity, and a stop valve is provided at the bottom of the cavity;

[0008] The liquid storage cavity is T-shaped as a whole, and the outlet of the liquid storage cavity close to the trigger valve is connected to the side of the main passage. The width of the liquid storage cavity is a, the length of the liquid storage cavity outlet is d, the width is b, and the depth is c, and follows the following rules: a=0.65mm±0.1mm, b=0.2mm±0.1mm, c=0.1mm±0.1mm, d=0.5mm±0.1mm;

[0009] The length of the liquid storage cavity is x, the distance between storage unit A, storage unit B and storage unit C is y, the mixing length of the solutions after contact inside the main passage is z, and follows the following rules: x=0.8mm±1mm, y=3mm±0.5mm, z=3mm±0.5mm. By using the above-mentioned size setting, the mixing efficiency between the storage unit and the main passage can be maximized. By changing the distance between the reagents, there is no obvious stratification problem between the reagents.

[0010] In a preferred embodiment, the bottom end of the cavity is connected to the main passage through the stop valve.

[0011] In a preferred embodiment, the arc-shaped cavity A, the arc-shaped cavity B, the arc-shaped cavity C and the circular cavity together form an integral mixing channel.

[0012] In a preferred embodiment, the volume values ​​of the arc-shaped cavity A, arc-shaped cavity B, arc-shaped cavity C and circular cavity are A, B, C, and D respectively, and follow the following rules: AC>D, A>C.

[0013] In a preferred embodiment, the overall structures of the storage unit A, storage unit B and storage unit C are the same.

[0014] In a preferred embodiment, the overlapping part of the arc cavity A and the arc cavity B is ≤ half of its own volume, and the overlapping part of the arc cavity C and the circular cavity is ≥ half of its own volume. By utilizing the volume and overlapping part of the arc cavity A8, the arc cavity B9, the arc cavity C10 and the circular cavity 11, when multiple solutions pass through the mixing channel composed of the arc cavity A8, the arc cavity B9, the arc cavity C10 and the circular cavity 11 after mixing, the volume of one side of the channel suddenly expands, thereby forming a secondary flow, thereby increasing the contact area between the solutions, and the suddenly increased channel cross-sectional area can further shorten the length between different solutions, reduce the solution stratification phenomenon and the problem of uneven mixing, and at the same time, the volume of the tail end of the mixing channel is suddenly reduced compared to the head end, so that after the rapid mixing, the solutions are collapsed, mixed and compressed together, thereby further improving the solution mixing effect, irregularly disrupting the flow direction between the solutions, and further ensuring the uniformity of the solution output.

[0015] Technical effects and advantages of the present invention:

[0016] 1. The present invention sets a plurality of storage units, a main channel and a capillary pump; by utilizing the mutual cooperation of the structures thereof, a plurality of reagents can be discharged in sequence, mixed with the blood sample in the main channel, and transported to the mixing channel after being concentrated (the mixing channel is composed of an arc cavity, an arc cavity, an arc cavity and a circular cavity as a whole). The mixing channel mainly forms a multi-dimensional secondary flow effect by forming an arc shape and a semicircle shape inside the mixing channel, thereby increasing the contact area between the plurality of reagents and the blood sample, and the suddenly increased channel cross-sectional area can further shorten the length between different reagents to avoid stratification between the reagents. While increasing the overall mixing performance of the structure, the utilization rate of the microfluidic chip can be improved, further improving its practicality and the promotion scope of the application of the structure. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0018] Figure 2 It is a schematic diagram of the partial cross-sectional structure of multiple liquid storage chambers and the main passage of the present invention;

[0019] Figure 3It is a schematic diagram of a partial three-dimensional structure of the present invention;

[0020] Figure 4 It is a schematic diagram of the partial structure of the main passage of the present invention;

[0021] Figure 5 It is a schematic diagram of the overall size structure of the liquid storage chamber of the present invention;

[0022] Figure 6 Schematic diagram of the dimensions of the liquid storage cavity of the present invention, the length x, the distance y between the storage unit A, the storage unit B and the storage unit C, and the mixing length z after the solutions in the main passage come into contact;

[0023] Figure 7 It is a finite element analysis cloud diagram of local reagent flow when a single storage unit reagent of the present invention contacts the main passage;

[0024] Figure 8 It is a schematic diagram of the use of the width a of the liquid storage cavity of the present invention under different sizes;

[0025] Fig. 9 Schematic diagram of the use of the width b of the liquid storage cavity outlet of the present invention under different sizes;

[0026] Fig.10 It is a schematic diagram of the use of the depth c of the liquid storage cavity of the present invention under different sizes;

[0027] Fig.11 It is a schematic diagram of the use of the liquid storage chamber of the present invention with a length d under different sizes;

[0028] Fig.12 It is a schematic diagram of the overall structure of Example 2 of the present invention.

[0029] The figures are marked as follows: 1. main passage; 2. input port; 3. outlet; 4. capillary pump; 5. storage unit A; 6. storage unit B; 7. storage unit C; 51. liquid storage chamber; 52. inlet; 53. trigger valve; 54. cavity; 55. burst valve; 56. stop valve; 8. arc cavity A; 9. arc cavity B; 10. arc cavity C; 11. circular cavity; 12. concentrated channel. DETAILED DESCRIPTION

[0030] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0031] Example 1

[0032] As attached Figure 1 To Attachment Fig.11 A self-driven micro-hybrid chip is shown, comprising a main passage 1, wherein an input port 2 and an outlet port 3 are respectively provided at two ends of the main passage 1, one end of the outlet port 3 is connected to a capillary pump 4, and the side of the main passage 1 is connected to storage units A5, B6 and C7 in sequence from left to right;

[0033] The tail end of the main passage 1 is connected with an arc cavity A8, an arc cavity B9, an arc cavity C10 and a circular cavity 11 in sequence from one side of the input port 2 to the side of the outlet 3. The axial angles between the arc cavity A8, the arc cavity B9, the arc cavity C10 and the circular cavity 11 are vertically distributed, and the arc cavity A8 and the circular cavity 11 are located on the same plane. The surface of the main passage 1 between the arc cavity B9 and the arc cavity C10 is oppositely distributed. There is an overlapping part in the horizontal position direction of the arc cavity A8 and the arc cavity B9. The side close to the arc cavity B9 and the arc cavity C10 is located on the same horizontal plane. There is an overlapping part in the horizontal position direction of the arc cavity C10 and the circular cavity 11.

[0034] The storage unit A5 comprises a liquid storage cavity 51, an inlet 52 is provided at one side of the top of the liquid storage cavity 51, a trigger valve 53 is provided at the bottom of the liquid storage cavity 51, a cavity 54 is provided on the side of the liquid storage cavity 51, a burst valve 55 is provided between the cavity 54 and the liquid storage cavity 51, and a stop valve 56 is provided at the bottom of the cavity 54;

[0035] The liquid storage chamber 51 is T-shaped as a whole, and the outlet of the liquid storage chamber 51 close to the trigger valve 53 is connected to the side of the main passage 1. The width of the liquid storage chamber 51 is a, the length of the outlet of the liquid storage chamber 51 is d, the width is b, and the depth is c, and follows the following rules: a=0.65mm±0.1mm, b=0.2mm±0.1mm, c=0.1mm±0.1mm, d=0.5mm±0.1mm;

[0036] Among the various parameters of the liquid storage chamber 51 structure, the width a of the liquid storage chamber 51 is the most important factor affecting the outflow of the solution in the liquid storage chamber 51. On the basis that the depth of the liquid storage chamber 51 is the same as the depth of the main passage 1, the size of the parameter a must be greater than the width of the main passage 1, so that the capillary force on the main passage 1 is much smaller than the capillary force on the liquid storage chamber 51, so as to ensure the normal outflow of the solution in the liquid storage chamber 51. The power of the entire microfluidic chip mainly comes from the capillary pump 4, which is completely affected by the capillary force. Due to various errors in the preparation of the microfluidic chip and the differences in operation, each operation process of the chip has certain differences, and the most important difference is caused by the difference in the driving force generated by the capillary pump 4. Different driving forces generated by the fine pumps result in different speeds of liquid flow in the microfluidic chip. In order to determine the influence of speed on the unit structure and determine the optimal parameters, the outlet speed is changed while changing the width a of the liquid storage chamber 51. The performance of the structure is determined by the inlet speed of the burst valve 55. As the width a increases, the normal outflow state of the liquid storage chamber 51 is less affected by the speed. When a is greater than or equal to 0.65, the outflow of the solution in the liquid storage chamber 51 is basically not affected by the speed. The smaller the length of the width a, the more difficult it is for the solution in the liquid storage chamber 51 to flow out regardless of whether the speed is fast or slow. Taking into account the optimal width of the liquid storage chamber 51 when the width a=0.65 is mixed with multiple solutions, On the basis of ensuring that the solution in the liquid storage chamber 51 can flow out normally, COMSOL simulation is used to demonstrate the influence of the outlet width b, depth c and length d of the liquid storage chamber 51 on the flow state of the solution in the liquid storage chamber 51. Since the outlet channel of the liquid storage chamber 51 also has the function of triggering the valve 53, the parameters b, c and d are reasonably optimized and selected under comprehensive consideration. The outlet width b of the liquid storage chamber 51 is changed, and the inlet speed of the bursting valve 55 and the flow speed of the solution in the liquid storage chamber 51 are compared to demonstrate the influence of the width b on the flow of the solution in the liquid storage chamber 51. It can be concluded that with the increase of the width b, within the same time, the outflow speed of the solution in the liquid storage chamber 51 first increases, then decreases, and then increases. In the selected width Within the range of b, when b=0.2mm, the outflow speed of the solution in the liquid storage chamber 51 reaches the fastest. Excessive width b will increase the possibility of premature outflow of the solution in the liquid storage chamber 51, and will cause a large error in the volume of the solution in the liquid storage chamber 51 in the subsequent process, thereby indirectly affecting the mixing performance. In the experiment, it was found that b=0.2mm has better performance while ensuring the outflow speed of the solution, which can well meet the needs of the device. By changing the outlet depth c of the liquid storage chamber 51 and comparing the inlet speed of the burst valve 55, the influence of the width b on the flow of the solution in the liquid storage chamber 51 is demonstrated. In the process of gradually increasing the depth c, the flow speed of the solution in the liquid storage chamber 51 first increases and then decreases, and at the depth c=0.When the length d of the liquid storage chamber 51 is 1 mm, the flow velocity of the solution in the liquid storage chamber 51 reaches the maximum. The change of the length d of the liquid storage chamber 51 affects the flow state of the solution in the liquid storage chamber 51. As the length d increases, the flow velocity of the solution in the liquid storage chamber 51 decreases. The increase in the outlet length d of the liquid storage chamber 51 will also greatly increase the size of the microfluidic chip, thereby reducing the utilization rate of the microfluidic chip. When d=0.5 mm, the structure achieves the best effect.

[0037] In summary, a=0.65mm, b=0.2mm, c=0.1mm, d=0.5mm are selected as the best parameters. At this time, the automation and sequential outflow of the solution are the best. The experimental data of multiple groups refer to Figures 8 to 11 shown.

[0038] The length of the liquid storage chamber 51 is x, the distance between the storage unit A5, the storage unit B6 and the storage unit C7 is y, and the mixing length of the solutions in the main passage 1 after contact is z, and follows the following rules: x=0.8mm±1mm, y=3mm±0.5mm, z=3mm±0.5mm;

[0039] In order to demonstrate the influence of the x, y and z parameters on the mixing effect, in the pigment experiment and the sodium hydroxide and phenolphthalein experiments, by changing the length x of the liquid storage chamber 51, the distance y between each storage unit and the length z of the mixture, as the length x of the liquid storage chamber 51 increases, the amount of solution stored in the liquid storage chamber 51 increases, which will greatly affect the mixing effect between the solutions. In the process of increasing x, the length of the mixed color increases, and the color produces an obvious light-dark-light distribution, indicating that as the amount of reagent increases, the amount of the mixed solution increases, but the mixing efficiency decreases. In the subsequent mixing of multiple solutions, the amount of solution will increase greatly, as shown in the attached figure. Figure 7 As shown, in order to maximize the mixing efficiency, the smallest x should be selected. By changing the distance between each solution, there is no obvious color stratification phenomenon, but the length of the mixed color area has a certain difference. As the distance between the liquid storage chambers 51 and the liquid storage chambers 51 increases, the mixed color area first increases and then decreases. Figure 8 As shown in the figure, it reaches the maximum when y=3mm. By changing the mixing distance z, as the mixing distance z increases, the length of the mixed color area and the depth of the mixed color first increase and then decrease, as shown in the figure below. Fig. 9 As shown, it reaches the maximum when z=3mm. In summary, the distance y between the liquid storage chambers 51 and the mixing distance z are both 3mm as the best. The length of the liquid storage chamber 51 needs to be reasonably selected according to actual conditions, and is generally set to x=0.8mm.

[0040] The bottom end of the cavity 54 is connected to the main passage 1 through the stop valve 56. The arc cavity A8, arc cavity B9, arc cavity C10 and circular cavity 11 together form an integral mixing passage. The overall structures of the storage unit A5, storage unit B6 and storage unit C7 are the same.

[0041] By setting up multiple storage units, it is possible to mix three or more types of solutions and ensure the mixing uniformity of each solution, thereby further improving the practicality and working effect of the device.

[0042] For details, please refer to the attached manual Figure 3-4 The volume values ​​of the arc cavity A8, arc cavity B9, arc cavity C10 and circular cavity 11 are A, B, C, and D respectively, and follow the following rules: AC>D, A>C, the overlapping part of the arc cavity A8 and the arc cavity B9 is ≤ half of its own volume, and the overlapping part of the arc cavity C10 and the circular cavity 11 is ≥ half of its own volume.

[0043] The specific implementation method is as follows: by utilizing the volume and overlapping parts of the arc cavity A8, the arc cavity B9, the arc cavity C10 and the circular cavity 11, when multiple solutions pass through the mixing channel composed of the arc cavity A8, the arc cavity B9, the arc cavity C10 and the circular cavity 11 after mixing, the volume of one side of the channel suddenly expands, thereby forming a secondary flow, and thereby increasing the contact area between the solutions. The suddenly increased channel cross-sectional area can further shorten the length between different solutions, reduce the solution stratification phenomenon and the problem of uneven mixing. At the same time, the volume of the tail end of the mixing channel is suddenly reduced compared to the head end, so that after the rapid mixing, the solutions are collapsed, mixed and compressed together, thereby further improving the solution mixing effect, irregularly disrupting the flow direction between the solutions, and further ensuring the uniformity of the solution output.

[0044] Among them, the size design of the trigger valve 53, the burst valve 55 and the stop valve 56 are all capillary channels. When there is liquid in the narrow channels of the trigger valve 53 and the burst valve 55, a certain degree of capillary force will be formed to prevent the formation of a closed state, so that the solution in the middle liquid storage chamber 51 remains still and no longer flows, thereby achieving the effect of temporarily storing the solution.

[0045] Working principle of the present invention:

[0046] Step 1: First, the operator assembles the various components of the device normally, and then uses the device normally.

[0047] Step 2: Multiple reagents (solutions) are sequentially added to multiple storage units, i.e., storage unit A, storage unit B, and storage unit, through the inlet position, and then the reagents flow into the narrow channel of the trigger valve 53. Since the size design of the trigger valve 53 and the burst valve 55 are both capillary channels, when there is liquid in the narrow channels of the trigger valve 53 and the burst valve 55, a certain degree of capillary force is formed to prevent the formation of a closed state, so that the reagent (solution) in the middle liquid storage chamber 51 remains stationary, thereby achieving the effect of temporarily storing the reagent. Subsequently, the collected blood sample is added to the inside of the main passage 1 through the input port 2. At this time, the blood sample flows to the right along the main passage 1, and gradually enters the deeper part of the main passage 1 and the rear end by the arc-shaped cavity A8, driven by the capillary pump 4. The arc cavity B9, the arc cavity C10 and the circular cavity 11 are together formed into the overall mixing channel. Meanwhile, as the blood sample gradually goes deeper, the blood sample can contact the trigger valve 53 at the outlet of the liquid storage cavity 51 below the storage unit A5, so that the liquid in the liquid storage cavity 51 and the blood sample inside the main passage 1 are interconnected, and a viscous connection is formed between them, so that the trigger valve 53 is kept in a ready-to-be-activated state (but at this time, the reagent in the liquid storage cavity 51 cannot be compensated by air from the outside and is in a nearly closed state, and the reagent still cannot flow out normally). When the blood sample in the main passage 1 is continuously driven by the capillary pump 4, when the tail end of the blood sample flows through the lower inlet of the cavity 54 (i.e., the position of the stop valve 56), the port of the stop valve 56 fails to be activated. The liquid barrier is removed and the liquid is connected to the input port 2 at the left end of the main passage 1. Since the input port 2 is directly connected to the outside atmosphere, a gas channel of the input port 2-main passage 1-stop valve 56-cavity 54-explosion valve 55-liquid storage chamber 51 is formed. At this time, the outside air is in direct contact with the explosion valve 55, so that the inside of the liquid storage chamber 51 can interact with the outside air, so that the reagent in the liquid storage chamber 51 has the basic conditions for flowing out. Under the continuous driving of the capillary pump 4, the capillary force generated by the liquid storage chamber 51 is much smaller than the capillary force generated in the main passage 1, and the reagent in the liquid storage chamber 51 flows first before the liquid in the main passage 1, that is, the reagent in the liquid storage chamber 51 is released to flow out and mixed with the blood sample inside the main passage 1, and the above principle is adopted to stimulate the subsequent The subsequent storage units B6 and C7 allow the various reagents to be gradually mixed into the blood sample and undergo a subsequent centralized mixing process. At this point, the reagents are self-driven and the process of flowing out in sequence is completed, thereby achieving the basic process of self-driven mixing of small doses and multiple reagents. Subsequently, after the multiple reagents are sequentially excited and mixed into the blood sample, they are concentrated together and then transported to the mixing channel (the mixing channel is composed of the arc cavity A8, the arc cavity B9, the arc cavity C10 and the circular cavity 11 as a whole). The mixing channel is mainly composed of the arc and semicircle formed by the arc cavity A8, the arc cavity B9, the arc cavity C10 and the circular cavity 11 to form a multi-dimensional secondary flow effect, thereby increasing the contact area between the reagent and the blood sample.The suddenly increased channel cross-sectional area can further shorten the length between different reagents to reduce the stratification of reagents, while increasing the overall mixing performance of the structure and improving the utilization rate of the microfluidic chip. Finally, the device completes the self-driven mixing process of small doses and multiple reagents.

[0048] Step 3: First, the operator shuts down the device normally, then checks whether the fixation between the various components of the device is normal, and then replaces and repairs the aging and severely worn parts inside the device.

[0049] Example 2

[0050] As attached Fig.12 As shown, first, multiple storage units A5 are arranged in sequence and in opposite distribution, and multiple main channels 1 are arranged in a forked shape and connected to the left and right ends of the concentrated channel 12. At the same time, the capillary pump 4 is set at the end of the concentrated channel 12 to perform mixing work in sequence. The performance of the self-driven microfluidic chip is further demonstrated by mixing more reagents. Since the concentrated channel 12 provides a longer mixing distance for the reagents and blood samples to be mixed, the utilization rate of the microfluidic chip can be improved while increasing the mixing performance. In the mixing experiment of multiple different reagents, the states of different reagents flow out and mix normally, and no obvious stratification phenomenon occurs. This establishes a better foundation for the subsequent microfluidic platform of the self-driven micro-mixing network, which can further expand the working effect under multi-reagent mixing and meet the mixing needs of multiple types of reagents in practical applications.

[0051] Finally, a few points should be explained: First, in the description of this application, it should be noted that, unless otherwise specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, which may refer to mechanical connection or electrical connection, or internal communication between two components, or direct connection. "upper", "lower", "left", "right", etc. are only used to indicate relative positional relationships. When the absolute position of the object being described changes, the relative positional relationship may change;

[0052] Secondly: In the drawings of the embodiments disclosed in the present invention, only the structures related to the embodiments disclosed in the present invention are involved, and other structures can refer to the general design. In the absence of conflict, the same embodiment and different embodiments of the present invention can be combined with each other;

[0053] Finally: The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A self-driven micro-hybrid chip, comprising a main passage, wherein both ends of the main passage are provided with an input port and an outlet, respectively, and one end of the outlet is connected to a capillary pump, characterized in that: The side of the main passage is connected with storage unit A, storage unit B and storage unit C in sequence from left to right; The tail end of the main passage is connected with arc cavity A, arc cavity B, arc cavity C and circular cavity in sequence from one side of the input port to the other side of the outlet port, the axial angles between the arc cavity A, arc cavity B, arc cavity C and circular cavity are vertically distributed, and the arc cavity A and the circular cavity are located on the same plane, the surfaces of the main passage between the arc cavity B and the arc cavity C are oppositely distributed, the arc cavity A and the arc cavity B have overlapping parts in the horizontal position direction, the arc cavity B and the arc cavity C are close to each other on the same horizontal plane, and the arc cavity C and the circular cavity have overlapping parts in the horizontal position direction; The storage unit A comprises a liquid storage cavity, an inlet is provided at one side of the top of the liquid storage cavity, a trigger valve is provided at the bottom of the liquid storage cavity, a cavity is provided on the side of the liquid storage cavity, a burst valve is provided between the cavity and the liquid storage cavity, and a stop valve is provided at the bottom of the cavity; The liquid storage cavity is T-shaped as a whole, and the outlet of the liquid storage cavity close to the trigger valve is connected to the side of the main passage. The width of the liquid storage cavity is a, the length of the liquid storage cavity outlet is d, the width is b, and the depth is c, and follows the following rules: a=0.65mm±0.1mm, b=0.2mm±0.1mm, c=0.1mm±0.1mm, d=0.5mm±0.1mm; The length of the liquid storage cavity is x, the distance between storage unit A, storage unit B and storage unit C is y, the mixing length of the solutions in the main passage after contact is z, and follows the following rules: x=0.8mm±1mm, y=3mm±0.5mm, z=3mm±0.5mm; The volume values ​​of the arc cavity A, arc cavity B, arc cavity C and circular cavity are A, B, C, and D respectively, and follow the following rules: AC>D, A>C, the overlapping part of the arc cavity A and the arc cavity B is ≤ half of their own volume, and the overlapping part of the arc cavity C and the circular cavity is ≥ half of their own volume.

2. The self-driving micro-hybrid chip according to claim 1, characterized in that: The bottom end of the cavity is connected to the main passage through the stop valve.

3. The self-driving micro-hybrid chip according to claim 1, characterized in that: The arc-shaped cavity A, arc-shaped cavity B, arc-shaped cavity C and circular cavity together form an integral mixing channel.

4. The self-driving micro-hybrid chip according to claim 1, characterized in that: The overall structures of the storage unit A, storage unit B and storage unit C are the same.

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