A reaction device and method for biological enzyme culture

Abstract

The present invention relates to the field of biological enzyme culture technology, and specifically, to a reaction device and method for biological enzyme culture. The device comprises a culture tank and a support frame, wherein a feed port is provided on the top of the culture tank, a motor is provided on the top of the culture tank, a connecting rod is provided at the output end of the motor, a stirring blade is provided near the bottom of the connecting rod, grooves are provided at the surface quadrants of the connecting rod, a defoaming component is provided inside the groove, an inflatable component is provided outside the culture tank, the inflatable component is arranged in a ring array on the side wall of the culture tank, and the inflatable component is used to squeeze air into the bottom of the culture tank to provide oxygen for biological enzyme culture. The present invention squeezes the wedge block through a pressing block, and the wedge block drives the sliding rod to slide on the inner wall of the first pipeline, squeezes the gas inside the first pipeline, and discharges the gas from the end of the second pipeline through a one-way valve, and blows and mixes the inside of the nutrient solution, thereby reducing the impact force on the biological enzyme in the nutrient solution, thereby improving the survival rate of the biological enzyme.

Description

Technical Field

[0001] The present invention relates to the technical field of biological enzyme cultivation, in particular to a reaction device and method for biological enzyme cultivation. Background Art

[0002] A biofermentation tank is a bioreactor that completes the fermentation process through solid culture and deep immersion culture. It can provide a suitable reaction environment for living cells or enzymes, thereby increasing the amount of bio-enzyme generated during the bio-enzyme fermentation reaction.

[0003] There are many existing technologies for fermentation tanks, such as:

[0004] Chinese patent publication number CN208577701U discloses a culture reactor for microbial fertilizer production equipment, including a reactor barrel and a barrel, the lower part of the reactor barrel is provided with legs, the lower middle part of the reactor barrel is provided with a discharge hole, the lower left and right sides of the reactor barrel are fixedly installed with blocks, the inner end of the block is provided with a slot, the inner sliding plug cover plate of the slot, the lower end of the cover plate is fixedly connected with a pull rod, and the upper left side of the reactor barrel is provided with a feed hole. The rotation of the stirring plate forces the bacterial agent to rub against the internal pores of the mesh circular plate, and various bacterial agents can be fully mixed during the friction, so that the bacterial agent is granulated and falls from the internal pores of the mesh circular plate to the bottom of the reactor barrel, so that the bacterial agents are mixed more evenly, which is beneficial to increase the production of aerobic bacteria. When air passes through the carbon gaps of the activated carbon layer, toxic impurities and various bacteria in the air are adsorbed inside it, protecting the growth environment of aerobic bacteria.

[0005] At present, in the process of using traditional fermentation tanks, the biological enzymes are cultured by mixing the biological enzymes and the culture solution and injecting them into the fermentation device. However, during the culture process, the nutrients in the culture solution are precipitated to the bottom of the fermentation tank due to gravity. When the nutrient solution is stirred by the stirring fan blades, the strong stirring may cause direct mechanical impact and shear force on the biological enzymes, causing the biological enzymes to die. In addition, bubbles are generated during the stirring process, which affects the contact area between oxygen and the biological enzymes in the nutrient solution, and easily leads to the activity of the biological enzymes.

[0006] Therefore, a reaction device and method for culturing biological enzymes are proposed. Summary of the invention

[0007] The object of the present invention is to provide a reaction device and method for culturing biological enzymes to solve the problems raised in the above-mentioned background technology.

[0008] In order to solve the above technical problems, one of the purposes of the present invention is to provide a reaction device for biological enzyme culture, including a culture tank and a support frame, the top of the culture tank is provided with a feed port, the surface of the culture tank is provided with an observation column, the top of the culture tank is provided with a motor, the output end of the motor is provided with a connecting rod, the connecting rod is provided with a stirring blade near the bottom, the surface quadrants of the connecting rod are provided with grooves, the grooves are provided with a defoaming component, the outside of the culture tank is provided with an inflation component, the inflation component is arranged in a circular array on the side wall of the culture tank, the inflation component is used to squeeze air into the bottom of the culture tank to provide oxygen for biological enzyme culture, at the same time, the inflation component impacts the nutrients precipitated at the bottom of the culture tank to make them float, so that the nutrients are evenly mixed in the biological enzyme, the side wall of the inflation component is provided with a filter component, the filter component is used to filter the air, a circular track is provided between the support frames, a moving body is slidably provided in the circular track, a pressing block is provided at the bottom of the moving body, and the moving body squeezes the inflation component when the pressing block moves.

[0009] As a further improvement of the present technical solution, the inflation component includes a first pipe arranged outside the culture tank, a second pipe is provided at the end of the first pipe, the second pipe passes through the culture tank to the interior thereof, and a one-way valve is provided between the first pipe and the second pipe.

[0010] As a further improvement of the technical solution, a sliding rod is provided inside the first pipe for sliding movement, a return spring is provided between the bottom of the sliding rod and the first pipe, a sealing rubber is provided on the outer wall of the sliding rod, and the sealing rubber is used to seal the gap between the sliding rod and the first pipe, and a wedge block is provided on the top of the sliding rod, and the height of the wedge block is higher than the pressure block.

[0011] As a further improvement of the technical solution, a nozzle is provided at the end of the second pipe, and the nozzle forms an angle of 60° with the horizontal plane. The nozzle impacts the nutrients precipitated at the bottom of the culture tank so that the nutrients are evenly mixed in the nutrient solution in the culture tank.

[0012] As a further improvement of the present technical solution, a through groove is formed on the side wall of the first pipe, a filter plate is formed at the end of the through groove, limit rods are formed at the top and bottom of the through groove, a filter assembly is formed outside the through groove, and the filter assembly includes a support plate arranged outside the through groove, a column tube is formed at the end of the support plate, a sealing plate is slidably provided on the inner wall of the column tube, the sealing plate is used for sealing at the through groove, and an elastic member is provided between the column tube and the sealing plate.

[0013] As a further improvement of the technical solution, a limiting groove is provided on the surface of the sealing plate, the limiting groove is matched with the limiting rod, and the limiting rod slides on the inner wall of the limiting groove when the sealing plate moves horizontally.

[0014] As a further improvement of the technical solution, the defoaming assembly includes a tooth plate arranged on the surface of the connecting rod, the tooth plate slides on the inner wall of the groove, and a telescopic spring is arranged between the tooth plate and the groove.

[0015] As a further improvement of the technical solution, a base plate is provided on the top of the tooth plate, an arc-shaped groove is opened on the surface of the base plate, a gas rod is provided above the base plate, and a circular pressure head is provided on the piston rod at the end of the gas rod, and the circular pressure head is fitted to the inner wall of the arc-shaped groove.

[0016] The second object of the present invention is to provide a method for using a reaction device for culturing a biological enzyme, comprising the reaction device for culturing a biological enzyme described in any one of the above, comprising the following steps:

[0017] S1, injecting the biological enzyme and culture solution into the culture tank from the feed port, driving the connecting rod to rotate by the motor, so that the stirring blades on the surface of the connecting rod stir and mix them;

[0018] S2. When the moving body drives the pressing block to move inside the circular track, when the pressing block moves to the inflation component, the pressing block squeezes the inflation component, so that air is discharged into the nutrient solution in the culture tank through the end of the inflation component, and the inflation component blows air into the nutrient solution to drive the nutrient solution to mix, and the dust and impurities in the external air are filtered through the filter component to prevent the culture solution in the culture tank from being contaminated by dust;

[0019] S3. Observe the position of the nutrient solution through the observation column. The defoaming component defoams the bubbles generated by the inflation component in the nutrient solution to prevent the bubbles from occupying the internal space of the culture tank and increase the contact area between the oxygen in the air and the biological enzyme.

[0020] Compared with the prior art, the present invention has the following beneficial effects:

[0021] 1. In the reaction equipment for biological enzyme culture, the wedge block is squeezed by the pressing block, and the wedge block drives the sliding rod to slide on the inner wall of the first pipe, squeezing the gas inside the first pipe, so that the gas is discharged from the end of the second pipe through the one-way valve, blowing air into the nutrient solution, and then mixing the nutrient solution, reducing the impact force on the biological enzyme in the nutrient solution, thereby improving the survival rate of the biological enzyme.

[0022] 2. In the reaction equipment for biological enzyme cultivation, when the extrusion of the internal gas of the first pipeline is completed, the sliding rod is reset by the force of the reset spring. At this time, the first pipeline is in a negative pressure state near the bottom. Under the extrusion of the external atmospheric pressure, the sealing plate slides horizontally on the inner wall of the through groove. At this time, the limiting rod at the through groove moves to the inner wall of the limiting groove to limit it, so that the limiting groove and the through groove are in an open state, allowing external air to enter the first pipeline, and the squeezed wedge block can be quickly reset, thereby quickly increasing the oxygen content inside the nutrient solution.

[0023] 3. In the reaction equipment for biological enzyme culture, the position of the nutrient solution is observed through the observation column, and the piston rod at the end of the gas rod drives the circular pressure head to squeeze the inner wall of the arc groove. The arc groove is squeezed and drives the base plate to move downward, so that the base plate drives the tooth plate to slide on the surface of the connecting rod. When the connecting rod is driven to rotate by the motor, the tooth plate degasses the bubbles floating on the surface of the nutrient solution, thereby preventing the bubbles from occupying the internal space of the culture tank, increasing the contact area between oxygen in the air and the biological enzyme, and improving the activity of the biological enzyme during the culture process. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0025] Figure 2 It is a cross-sectional view of the overall structure of the present invention;

[0026] Figure 3 is a cross-sectional view of an inflatable component of the present invention;

[0027] Figure 4 It is a schematic diagram of the structure of the mobile body of the present invention;

[0028] Figure 5 For the present invention Figure 3 Schematic diagram of point A;

[0029] Figure 6 It is a schematic diagram of the structure of the defoaming group of the present invention;

[0030] Figure 7 For the present invention Figure 6 Schematic diagram of point B;

[0031] Figure 8 It is a schematic diagram of the briquetting structure of the present invention;

[0032] Fig. 9 It is the overall structural front view of the present invention.

[0033] The meaning of each number in the figure is:

[0034] 100, culture tank; 101, motor; 102, connecting rod; 103, stirring blade; 104, groove; 105, observation column;

[0035] 200, support frame; 201, circular track; 202, moving body; 203, pressing block;

[0036] 300, inflation assembly; 301, first pipeline; 302, second pipeline; 303, one-way valve; 304, sealing rubber; 305, return spring; 306, sliding rod; 307, wedge block.

[0037] 400, filter assembly; 401, support plate; 402, column; 403, sealing plate; 404, elastic member; 405, limiting groove; 406, filter plate;

[0038] 500, defoaming assembly; 501, tooth plate; 502, base plate; 503, arc groove; 504, telescopic spring; 505, gas rod; 506, circular pressure head. DETAILED DESCRIPTION

[0039] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0040] like Figure 1 As shown, one of the purposes of the present invention is to provide a reaction device for biological enzyme culture, including a culture tank 100 and a support frame 200, a feed port is provided on the top of the culture tank 100, an observation column 105 is provided on the surface of the culture tank 100, a motor 101 is provided on the top of the culture tank 100, a connecting rod 102 is provided at the output end of the motor 101, a stirring blade 103 is provided near the bottom of the connecting rod 102, grooves 104 are provided at the surface quadrants of the connecting rod 102, a defoaming component 500 is provided inside the groove 104, an inflatable component 300 is provided outside the culture tank 100, and the inflatable component 300 is arranged in a ring array on the culture tank 100 The side wall of the inflatable component 300 is used to squeeze air into the bottom of the culture tank 100 to provide oxygen for the biological enzyme culture. At the same time, the inflatable component 300 impacts the nutrients precipitated at the bottom of the culture tank 100 to make them float, so that the nutrients are evenly mixed in the biological enzyme. The side wall of the inflatable component 300 is provided with a filter component 400, and the filter component 400 is used to filter the air. A circular track 201 is provided between the support frames 200, and a moving body 202 is slidably provided in the circular track 201. A pressure block 203 is provided at the bottom of the moving body 202. When the moving body 202 drives the pressure block 203 to move, it squeezes the inflatable component 300.

[0041] Considering that during the cultivation of biological enzymes, nutrients need to be added therein, including but not limited to carbon sources: such as glucose, sucrose or starch, to provide energy and carbon required for growth; nitrogen sources: such as ammonium salts, urea or protein sources, to provide nitrogen to synthesize enzymes and other cell components; minerals: such as potassium, calcium, magnesium, iron, etc., which are very important for the stability and activity of enzymes; vitamins and trace elements: such as biotin, thiamine, etc., which are very important for the metabolism of microorganisms and the synthesis of enzymes. When there are more nutrients, they are easily precipitated to the bottom of the culture tank 100 due to gravity, resulting in the influence of the reproduction speed during the cultivation of biological enzymes. Moreover, during the cultivation of biological enzymes, sufficient oxygen needs to be added therein to avoid the death of biological enzymes due to lack of oxygen. Therefore, the inflation component 300 includes a first pipe 301 arranged outside the culture tank 100, and a second pipe 302 is arranged at the end of the first pipe 301, and the second pipe 302 passes through the culture tank 100 to the inside thereof. A one-way valve 303 is provided between the first pipe 301 and the second pipe 302, a sliding rod 306 is slidably provided inside the first pipe 301, a return spring 305 is provided between the bottom of the sliding rod 306 and the first pipe 301, a sealing rubber 304 is provided on the outer wall of the sliding rod 306, and the sealing rubber 304 is used to seal the gap between the sliding rod 306 and the first pipe 301, and a wedge block 307 is provided on the top of the sliding rod 306, and the height of the wedge block 307 is higher than the pressing block 203. By inflating the first pipe 301, air passes through the one-way valve 303 and enters the second pipe 302, and the culture solution inside the culture tank 100 is blown through the second pipe 302 to increase the oxygen content inside the culture solution. At the same time, the air blows the nutrient solution at the bottom of the culture tank 100 through the second pipe 302, so that the nutrients precipitated to the bottom of the culture tank 100 are evenly mixed, so that the biological enzymes cultured in the nutrient solution can all absorb the nutrients, thereby improving the efficiency of biological enzyme reproduction.

[0042] During the cultivation of biological enzymes, when the number of biological enzymes is large, when the nutrient solution in the culture tank 100 is stirred and mixed by the stirring blade 103, the strong stirring may cause direct mechanical impact and shear force on the biological enzymes, causing the biological enzymes to die. In order to improve the survival rate of the biological enzymes, a sliding rod 306 is provided inside the first pipe 301 for sliding, a return spring 305 is provided between the bottom of the sliding rod 306 and the first pipe 301, and a sealing rubber 304 is provided on the outer wall of the sliding rod 306. The sealing rubber 304 is used to seal the gap between the sliding rod 306 and the first pipe 301. The top of the sliding rod 306 is provided with a wedge-shaped Block 307, the height of the wedge block 307 is higher than the pressing block 203. Therefore, when the moving body 202 drives the pressing block 203 to move inside the circular track 201, when the pressing block 203 moves to the wedge block 307, the wedge block 307 is squeezed by the pressing block 203, and the wedge block 307 drives the sliding rod 306 to slide on the inner wall of the first pipe 301, squeezing the gas inside the first pipe 301, so that the gas passes through the one-way valve 303 and is discharged from the end of the second pipe 302, blowing air into the nutrient solution, while mixing the nutrient solution, reducing the impact force on the biological enzyme in the nutrient solution, thereby improving the survival rate of the biological enzyme.

[0043] When the output end of the motor 101 drives the connecting rod 102 to rotate coaxially, the connecting rod 102 drives the stirring blade 103 to stir the nutrient solution. When the nutrient solution is stirred, centrifugal force is generated. The centrifugal force causes the heavier material particles to be subjected to an outward force. However, due to the obstruction of the tank body, they cannot move outward infinitely, and are subjected to a reaction force at the bottom of the tank body, which eventually causes the nutrients to gather toward the center of the bottom of the culture tank 100, resulting in the biological enzymes far away from the bottom of the culture tank 100 not being able to absorb enough nutrients for normal production and reproduction, affecting the overall biological enzyme culture efficiency. Therefore, a nozzle is provided at the end of the second pipe 302 The nozzle forms an angle of 60° with the horizontal plane, and the nozzle impacts the nutrients precipitated at the bottom of the culture tank 100, so that the nutrients are evenly mixed in the nutrient solution in the culture tank 100. When air enters the second pipe 302 and blows into the nutrient solution, since the nozzle arranged at the end of the second pipe 302 forms an angle of 60° with the horizontal plane, when the nutrient solution in the culture tank 100 is blown, the nutrients gathered at the bottom center of the culture tank 100 are blown away, so that they can be mixed in the nutrient solution, thereby ensuring the normal growth and reproduction of the biological enzymes away from the nutrient solution at the bottom of the culture tank 100, and improving the biological enzyme reproduction efficiency.

[0044] Taking into account that when the gas inside the first pipe 301 is pressed, in order to enable the nutrient solution in the culture tank 100 to be filled with oxygen and the nutrient solution to be stirred multiple times, a through groove is provided on the side wall of the first pipe 301, and limiting rods are provided at the top and bottom of the through groove. A filter assembly 400 is provided outside the through groove, and the filter assembly 400 includes a support plate 401 arranged outside the through groove, a column 402 is provided at the end of the support plate 401, and a sealing plate 403 is slidably provided on the inner wall of the column 402, and the sealing plate 403 is used for sealing at the through groove, and an elastic member 404 is provided between the column 402 and the sealing plate 403, and a limiting groove 405 is provided on the surface of the sealing plate 403, and the limiting groove 405 is adapted to the limiting rod. When the sealing plate 403 moves horizontally, the limiting rod slides on the inner wall of the limiting groove 405, and when the wedge block 307 is driven by the pressure of the pressing block 203 to drive the sliding rod 306 to slide on the inner wall of the first pipe 301, the sliding rod 306 The air inside the first pipe 301 is squeezed and discharged from the nozzle at the end of the second pipe 302 through the one-way valve 303. When the squeezing of the gas inside the first pipe 301 is completed, the slide bar 306 is reset by the force of the reset spring 305. At this time, the first pipe 301 is in a negative pressure state near the bottom. Under the squeeze of the external atmospheric pressure, the sealing plate 403 slides horizontally on the inner wall of the through groove. At this time, the limiting rod at the through groove moves to the inner wall of the limiting groove 405 to limit it, so that the limiting groove 405 and the through groove are in an open state, allowing external air to enter the first pipe 301. At the same time, dust and impurities in the external air are filtered through the filter plate 406 to prevent the culture solution inside the culture tank 100 from being contaminated by dust. When the moving body 202 drives the pressing block 203 to rotate on the surface of the circular track 201, the squeezed wedge block 307 can be quickly reset, thereby quickly increasing the oxygen content inside the nutrient solution.

[0045] Considering that when air is blown into the nutrient solution through the second pipe 302, bubbles and foam are easily generated inside the nutrient solution, and the foam will occupy a certain space, reduce the effective gas-liquid contact area in the culture tank 100, and affect the activity of the biological enzyme, therefore, the defoaming component 500 includes a tooth plate 501 arranged on the surface of the connecting rod 102, the tooth plate 501 slides on the inner wall of the groove 104, a telescopic spring 504 is arranged between the tooth plate 501 and the groove 104, a base plate 502 is arranged on the top of the tooth plate 501, an arc groove 503 is opened on the surface of the base plate 502, an air rod 505 is arranged above the base plate 502, and a piston rod at the end of the air rod 505 is provided with a circular pressure head 506, and the circular pressure head 506 is arranged on the piston rod 506. The circular pressure head 506 is attached to the inner wall of the arc groove 503, and the piston rod at the end of the control gas rod 505 drives the circular pressure head 506 to move downward. The position of the nutrient solution is observed through the observation column 105. The circular pressure head 506 squeezes the inner wall of the arc groove 503. The arc groove 503 is squeezed and drives the base plate 502 to move downward, so that the base plate 502 drives the tooth plate 501 to slide on the surface of the connecting rod 102. When the connecting rod 102 is driven to rotate by the motor 101, the tooth plate 501 degasses the bubbles floating on the surface of the nutrient solution, thereby preventing the bubbles from occupying the internal space of the culture tank 100, increasing the contact area between air and the biological enzyme, and improving the activity of the biological enzyme during the culture process.

[0046] When used specifically, the biological enzyme and the culture solution are injected into the culture tank 100 from the feed port, and the motor 101 drives the connecting rod 102 to rotate, so that the stirring blade 103 on the surface of the connecting rod 102 stirs and mixes them. When the moving body 202 drives the pressing block 203 to move inside the circular track 201, when the pressing block 203 moves to the wedge block 307, the pressing block 203 squeezes the wedge block 307, and the wedge block 307 drives the sliding rod 306 to slide on the inner wall of the first pipe 301, squeezes the gas inside the first pipe 301, and makes the gas pass through the one-way valve 303 and be discharged from the end of the second pipe 302, and blows air into the nutrient solution, and when the nutrient solution is mixed, the impact force on the biological enzyme in the nutrient solution is reduced, thereby improving the survival rate of the biological enzyme;

[0047] When the extrusion of the gas inside the first pipe 301 is completed, the sliding rod 306 is reset by the force of the reset spring 305. At this time, the first pipe 301 is in a negative pressure state near the bottom. Under the extrusion of the external atmospheric pressure, the sealing plate 403 slides horizontally on the inner wall of the through groove. At this time, the limiting rod at the through groove moves to the inner wall of the limiting groove 405 to limit it, so that the limiting groove 405 and the through groove are in an open state, allowing external air to enter the first pipe 301. At the same time, the dust and impurities in the external air are filtered by the filter plate 406 to prevent the culture solution inside the culture tank 100 from being contaminated by dust. When the moving body 202 drives the pressing block 203 to rotate on the surface of the circular track 201, the squeezed wedge block 307 can be quickly reset, thereby quickly increasing the oxygen content inside the nutrient solution.

[0048] The position of the nutrient solution is observed through the observation column 105, and the piston rod at the end of the gas rod 505 drives the circular pressure head 506 to squeeze the inner wall of the arc groove 503. The arc groove 503 is squeezed and drives the base plate 502 to move downward, so that the base plate 502 drives the tooth plate 501 to slide on the surface of the connecting rod 102. When the connecting rod 102 is driven to rotate by the motor 101, the tooth plate 501 degasses the bubbles floating on the surface of the nutrient solution, thereby preventing the bubbles from occupying the internal space of the culture tank 100, increasing the contact area between oxygen in the air and the biological enzyme, and improving the activity of the biological enzyme during the culture process.

[0049] The second object of the present invention is to provide a method for using a reaction device for biological enzyme cultivation, comprising any one of the above-mentioned reaction devices for biological enzyme cultivation, comprising the following steps:

[0050] S1, injecting the biological enzyme and the culture solution into the culture tank 100 from the feed port, driving the connecting rod 102 to rotate by the motor 101, so that the stirring blades 103 on the surface of the connecting rod 102 stir and mix them;

[0051] S2, when the moving body 202 drives the pressing block 203 to move inside the circular track 201, when the pressing block 203 moves to the inflatable component 300, the pressing block 203 squeezes the inflatable component 300, so that the air is discharged into the nutrient solution in the culture tank 100 through the end of the inflatable component 300, and the inflatable component 300 blows air into the nutrient solution to drive the nutrient solution to mix, thereby reducing the impact force on the biological enzyme in the nutrient solution, thereby improving the survival rate of the biological enzyme, and filtering the dust and impurities in the external air through the filter component 400 to prevent the culture solution in the culture tank 100 from being contaminated by dust;

[0052] S3. Observe the position of the nutrient solution through the observation column 105. The defoaming component 500 defoams the bubbles generated by the inflation component 300 in the nutrient solution to prevent the bubbles from occupying the internal space of the culture tank 100, increase the contact area between oxygen in the air and the biological enzyme, and improve the activity of the biological enzyme during the culture process.

[0053] The above shows and describes the basic principles, main features and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited by the above embodiments. The above embodiments and descriptions are only preferred examples of the present invention and are not intended to limit the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, which fall within the scope of the present invention. The scope of protection of the present invention is defined by the attached claims and their equivalents.

Claims

1. A reaction device for biological enzyme cultivation, characterized in that: The invention comprises a culture tank (100) and a support frame (200), wherein a feed port is provided on the top of the culture tank (100), an observation column (105) is provided on the surface of the culture tank (100), a motor (101) is provided on the top of the culture tank (100), a connecting rod (102) is provided at the output end of the motor (101), a stirring blade (103) is provided near the bottom of the connecting rod (102), grooves (104) are provided at the quadrants of the surface of the connecting rod (102), a defoaming component (500) is provided inside the groove (104), an inflation component (300) is provided outside the culture tank (100), the inflation components (300) are arranged in a ring array on the side wall of the culture tank (100), the inflation components (300) are used to squeeze air into the bottom of the culture tank (100) to provide oxygen for biological enzyme culture, and at the same time, the inflation component (300) impacts the nutrients precipitated at the bottom of the culture tank (100). The inflatable component (300) comprises a first pipe (301) arranged outside the culture tank (100), a second pipe (302) is arranged at the end of the first pipe (301), the second pipe (302) passes through the culture tank (100) to the inside thereof, a one-way valve (303) is arranged between the first pipe (301) and the second pipe (302), a sliding rod (306) is arranged inside the first pipe (301) for sliding, a return spring (305) is arranged between the bottom of the sliding rod (306) and the first pipe (301), a sealing rubber (304) is arranged on the outer wall of the sliding rod (306), the sealing rubber (304) is used to seal the gap between the sliding rod (306) and the first pipe (301), a wedge block (307) is arranged at the top of the sliding rod (306), and the height of the wedge block (307) is higher than the pressing block (203); A filter assembly (400) is provided on the side wall of the inflatable assembly (300), and the filter assembly (400) is used to filter air. A circular track (201) is provided between the support frames (200), and a moving body (202) is slidably provided in the circular track (201). A pressing block (203) is provided at the bottom of the moving body (202). When the moving body (202) drives the pressing block (203) to move, the inflatable assembly (300) is squeezed, and the pressing block (203) squeezes the wedge block (307). The wedge block (307) drives the sliding rod (306) to slide on the inner wall of the first pipe (301), squeezes the gas inside the first pipe (301), and discharges the gas from the end of the second pipe (302) through the one-way valve (303).

2. The bioenzyme culture reaction device according to claim 1, characterized in that: A nozzle is provided at the end of the second pipe (302), and the nozzle forms an angle of 60° with the horizontal plane. The nozzle impacts the nutrients precipitated at the bottom of the culture tank (100), so that the nutrients are evenly mixed in the nutrient solution in the culture tank (100).

3. The bioenzyme culture reaction device according to claim 1, characterized in that: A through slot is provided on the side wall of the first pipe (301), a filter plate (406) is provided at the end of the through slot, limiting rods are provided at the top and bottom of the through slot, a filter assembly (400) is provided outside the through slot, and the filter assembly (400) comprises a support plate (401) provided outside the through slot, a column (402) is provided at the end of the support plate (401), a sealing plate (403) is slidably provided on the inner wall of the column (402), the sealing plate (403) is used for sealing at the through slot, and an elastic member (404) is provided between the column (402) and the sealing plate (403).

4. The reaction equipment for biological enzyme cultivation according to claim 3, characterized in that: A limiting groove (405) is provided on the surface of the sealing plate (403), the limiting groove (405) is matched with a limiting rod, and when the sealing plate (403) moves horizontally, the limiting rod slides on the inner wall of the limiting groove (405).

5. The bioenzyme culture reaction device according to claim 1, characterized in that: The defoaming component (500) comprises a tooth plate (501) arranged on the surface of the connecting rod (102), the tooth plate (501) slides on the inner wall of the groove (104), and a telescopic spring (504) is arranged between the tooth plate (501) and the groove (104).

6. The reaction equipment for biological enzyme cultivation according to claim 5, characterized in that: A base plate (502) is provided on the top of the tooth plate (501), an arc-shaped groove (503) is provided on the surface of the base plate (502), a gas rod (505) is provided above the base plate (502), a piston rod at the end of the gas rod (505) is provided with a circular pressure head (506), and the circular pressure head (506) is attached to the inner wall of the arc-shaped groove (503).

7. A method for using a reaction device for biological enzyme cultivation, comprising the reaction device for biological enzyme cultivation according to any one of claims 1 to 6, characterized in that: The following steps are involved: S1, injecting the biological enzyme and the culture solution into the culture tank (100) from the feed port, driving the connecting rod (102) to rotate by the motor (101), so that the stirring blades (103) on the surface of the connecting rod (102) stir and mix them; S2, when the moving body (202) drives the pressing block (203) to move inside the circular track (201), when the pressing block (203) moves to the inflation component (300), the pressing block (203) squeezes the inflation component (300) so that air is discharged into the nutrient solution in the culture tank (100) through the end of the inflation component (300), and the inflation component (300) blows air into the nutrient solution to drive the nutrient solution to be mixed, and the dust impurities in the external air are filtered through the filter component (400) to prevent the culture solution in the culture tank (100) from being contaminated by dust; S3. The position of the nutrient solution is observed through the observation column (105), and the defoaming component (500) defoams the bubbles generated by the aeration component (300) in the nutrient solution to prevent the bubbles from occupying the internal space of the culture tank (100), thereby increasing the contact area between the oxygen in the air and the biological enzyme.

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