A testing device for extracting plant polysaccharides by enzymatic method

Abstract

The present invention belongs to the technical field of plant polysaccharide testing devices, and in particular, relates to a plant polysaccharide extraction testing device using an enzymatic method, comprising a spectrophotometer body, a detection cavity is provided on the top of the spectrophotometer body, a detection cover is hinged at the top opening of the detection cavity, a U-shaped partition is fixedly connected to the bottom inner wall of the detection cavity, and the opening of the U-shaped partition faces forward, a movable block is provided on the inner side of the U-shaped partition, and a pull rod is fixedly connected to the front end of the movable block horizontally, and the pull rod slides vertically through and is plugged into the front side of the spectrophotometer body. The present invention is provided with a turntable, so that multiple samples to be tested can be placed at one time, and multiple groups of parallel experiments can be completed by switching the positions of the samples to be tested. Compared with the existing detection method, the subsequent steps of opening the detection cover to replace the samples to be tested and repeatedly calibrating the detection probe are omitted, thereby greatly improving the detection efficiency.

Description

Technical Field

[0001] The invention belongs to the technical field of plant polysaccharide testing devices, and in particular relates to a testing device for extracting plant polysaccharides using an enzymatic method. Background Art

[0002] The principle of enzymatic extraction of polysaccharides lies in the specific catalytic effect of enzymes. Enzymes can selectively and specifically degrade the cell walls and cell membranes (such as starch, cellulose, pectin and other major cell components) of plants, algae and microorganisms, reduce the resistance from the cell walls, cell membranes and intercellular matrix during solvent extraction, and release the effective ingredients. At the same time, the use of enzymes can also degrade some polysaccharides into smaller molecular weight fragments, which is more conducive to the separation of polysaccharides from cells. The efficiency of enzymatic extraction of polysaccharides is generally related to factors such as temperature, pH, substrate concentration, enzyme concentration, extraction time, inhibitors and activators. In order to ensure the best effect of enzymatic extraction of polysaccharides, the enzymatic products are generally tested during the extraction of polysaccharides.

[0003] The existing method of polysaccharide detection is mainly to put the sample solution into a cuvette, and then use a spectrophotometer to detect the polysaccharide content in the sample. During the detection, in order to ensure the accuracy of the results, it is generally necessary to set up a control group and two test groups, and in the general detection process, it is necessary to set up three groups of parallel experiments, so it is necessary to perform multiple tests on one of the test group solutions, and finally take the average value of the three groups of test results to accurately obtain the polysaccharide content in the sample.

[0004] However, in the above detection process, since only three cuvettes can be placed in the existing spectrophotometer at a time, after completing a set of tests, it is necessary to open the spectrophotometer, take out one of the samples to be tested, replace it with the prepared sample to be tested, and re-test. Moreover, after opening the spectrophotometer and replacing the sample to be tested, since the detection probe in the spectrophotometer is relatively sensitive, in order to ensure the accuracy of the test results, it is necessary to recalibrate the spectrophotometer, which is very troublesome and time-consuming.

[0005] Therefore, it is necessary to invent an enzymatic extraction test device for plant polysaccharides to solve the above problems. Summary of the invention

[0006] In view of the above problems, the present invention provides a testing device for extracting plant polysaccharides by enzymatic method to solve the problems raised in the above background technology.

[0007] To achieve the above object, the present invention provides the following technical solutions:

[0008] A testing device for extracting plant polysaccharides by enzymatic method, comprising a spectrophotometer body, a detection cavity is opened on the top of the spectrophotometer body, a detection cover is hinged at the top opening of the detection cavity, a U-shaped partition is fixedly connected to the bottom inner wall of the detection cavity, and the opening of the U-shaped partition faces forward, a movable block is arranged on the inner side of the U-shaped partition, the front end of the movable block is horizontally fixedly connected to a pull rod, the pull rod vertically slides through and is inserted in the front side of the spectrophotometer body, the rear side of the movable block is horizontally fixedly connected to a support plate, and the bottom of the support plate is flush with the bottom of the movable block, two slide rails are arranged in parallel on the bottom of the movable block and the support plate, the slide rails are fixedly connected to the bottom inner wall of the detection cavity, and the slide rails are parallel to the side of the spectrophotometer body, two sliders are fixedly connected to the bottom of the movable block and the support plate, and the two sliders are slidably installed on the two slide rails respectively, a through hole is opened through the top of the support plate, and the top of the support plate is horizontally arranged There is a circular turntable, and the top of the turntable is flush with the top of the movable block. The bottom of the turntable is fixedly connected with a concentric support ring, and the support ring is rotatably installed on the top of the support plate. A fixed shaft is vertically fixedly connected at the center of the bottom circle of the support plate, and a one-way gear is fixedly sleeved on the fixed shaft near the bottom end. A rack is provided on one side of the one-way gear, and the rack is fixedly connected to the inner wall of the bottom of the detection chamber, and the teeth on the rack can mesh with the one-way gear. Three first detection grooves are evenly opened on the top of the turntable, and the three first detection grooves are distributed in a ring shape. Two second detection grooves are opened on the top of the movable block, and the two second detection grooves are distributed front and back. Colorimetric vessels are inserted in the first detection groove and the second detection groove, and a detection probe is installed on the side of the U-shaped partition. A correction component for correcting the turntable after rotation is provided on the rear side of the turntable, and a positioning component for positioning the movable block after movement is provided at the bottom of the movable block.

[0009] Furthermore, the correction component includes an isosceles triangle correction block, and the symmetry center line of the correction block is on the same straight line as the axis of the turntable, the rear side of the correction block is horizontally and vertically fixedly connected with a positioning rod, the positioning rod vertically slides through and is inserted into the rear side of the U-shaped partition, and limiting rods are symmetrically arranged on both sides of the positioning rod, the limiting rods are fixedly connected to the correction block, the rear end of the limiting rod slides through and is inserted into the rear side of the U-shaped partition, and the rear ends of the limiting rod and the positioning rod are fixed with limiting blocks, the positioning rod is sleeved with a clamping spring near the front end, and the two ends of the clamping spring are respectively fixedly connected to the correction block and the U-shaped partition, and three correction openings matching the correction block are opened on the side of the turntable, and the three correction openings are alternately distributed with the three first detection slots.

[0010] Furthermore, the positioning assembly includes a telescopic rod, which is vertically fixedly connected to the bottom inner wall of the detection chamber, and a support spring is sleeved on the telescopic rod, and the two ends of the support spring are respectively fixedly connected to the surfaces of the telescopic rod close to the two ends, and the top of the telescopic rod is fixedly connected to a spherical clamping block, and the bottom of the movable block is evenly distributed with three spherical clamping grooves matching the spherical clamping block, and the three spherical clamping grooves are distributed front to back, and the spherical clamping grooves and the spherical clamping block are located on the same motion trajectory, and the distance between two adjacent spherical clamping grooves is the same as the distance between the two second detection grooves. When the spherical clamping block is inserted into the spherical clamping groove on the front side, the detection probe is facing the side of the cuvette in the second detection groove on the front side.

[0011] Furthermore, when the correction block is plugged into one of the correction slots on the side of the turntable, the first detection slot and the two second detection slots facing the correction slot are located on the same straight line, and the three are equidistantly distributed.

[0012] Furthermore, a deflection groove is provided on the groove wall of the first detection groove away from the turntable and on the front groove wall of the second detection groove, and a bow-shaped spring piece is fixedly connected to the top groove wall of the deflection groove, both ends of the bow-shaped spring piece are located in the deflection groove, and the middle part of the bow-shaped spring piece is deflected outside the deflection groove.

[0013] Furthermore, the rear side of the movable block is designed as an arc surface, and the rear side of the movable block is in contact with the side of the turntable. A first groove is provided on the arc surface on the rear side of the movable block, and a first magnetic block is installed in the first groove. Three second grooves are evenly provided on the side of the turntable, and the three second grooves are opposite to the three first detection grooves one by one. A second magnetic block is installed in the second groove, and the magnetic pole of the second magnetic block is opposite to the magnetic pole on the opposite side of the first magnetic block.

[0014] Furthermore, the idling direction of the one-way gear is the same as the forward direction of the movable block, and the one-way gear can only drive the turntable to rotate one-third of a circle at a time under the action of the rack.

[0015] Furthermore, two light-proof baffles are vertically fixedly connected to the top of the movable block, and the two baffles are respectively located at the rear sides of the two second detection slots, and the height and width of the baffles are greater than the height and width of the cuvette.

[0016] Furthermore, the maximum elastic force of the compression spring on the deviation-correcting block is smaller than the resistance between the spherical clamping block and the spherical clamping groove.

[0017] Technical effects and advantages of the present invention:

[0018] 1. The present invention is provided with a turntable, so that multiple samples to be tested can be placed at one time, and multiple groups of parallel experiments can be completed by switching the positions of the samples to be tested. Compared with the existing detection method, the steps of opening the detection cover to replace the samples to be tested and repeatedly calibrating the detection probe are omitted, thereby greatly improving the detection efficiency;

[0019] 2. The present invention is provided with a correction component. After the position of the sample to be detected on the turntable is exchanged, as the turntable contacts the correction block, the correction block can cooperate with the correction port facing it to drive the turntable to rotate finely, so that the cuvette to be detected on the turntable can be kept in the same plane as the two cuvettes on the movable block, thereby ensuring that the detection probe can keep facing the cuvette rotated to the front side in the subsequent detection process, thereby ensuring the accuracy of the detection probe detection;

[0020] 3. The present invention is provided with a first magnetic block and a second magnetic block. When the turntable rotates one-third of a turn under the action of the rack and the one-way gear, the first magnetic block opposite to the second magnetic block can be attracted to each other, thereby locking the state of the turntable after rotation, avoiding the turntable from deflecting due to the vibration generated during movement when the movable block drives the turntable to rotate through the support plate, thereby ensuring that the detection probe can more accurately detect the solution in the cuvette on the top of the turntable, thereby ensuring the accuracy of the detection. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0022] Figure 2 It is a three-dimensional schematic diagram of all structures of the present invention except the detection cover;

[0023] Figure 3 It is a three-dimensional schematic diagram of the U-shaped partition and all structures inside it in the present invention;

[0024] Figure 4 It is a three-dimensional schematic diagram of all structures inside the U-shaped partition in the present invention;

[0025] Figure 5 It is a three-dimensional schematic diagram of the rotating disk, the movable block, the baffle, the bow-shaped spring piece and the second magnetic block in the present invention;

[0026] Figure 6 It is a three-dimensional schematic diagram of the support plate, the movable block, the bow-shaped spring piece, the first magnetic block and the sliding block in the present invention;

[0027] Figure 7 It is a three-dimensional schematic diagram of the positioning component in the present invention;

[0028] Figure 8 It is a three-dimensional schematic diagram of the turntable, the support ring, the second magnetic block, the fixed shaft, the one-way gear and the rack in the present invention.

[0029] In the figure: 1. spectrophotometer body; 2. detection cover; 3. U-shaped partition; 4. movable block; 5. pull rod; 6. support plate; 7. slide rail; 8. slider; 9. turntable; 10. support ring; 11. fixed shaft; 12. one-way gear; 13. rack; 14. first detection slot; 15. second detection slot; 16. cuvette; 17. detection probe; 18. correction assembly; 181. correction block; 182. positioning rod; 183. limit rod; 184. limit block; 185. compression spring; 186. correction mouth; 19. positioning assembly; 191. telescopic rod; 192. support spring; 193. spherical clamp block; 194. spherical clamp slot; 20. bow-shaped spring; 21. first magnetic block; 22. second magnetic block; 23. baffle. DETAILED DESCRIPTION

[0030] In order to make the purpose, technical solution and advantages of the embodiments of the present invention more clear, the technical solution of the present invention will be clearly and completely described below in conjunction with the embodiments.

[0031] The present invention provides Figures 1 to 8The device for testing the extraction of plant polysaccharides by enzymatic method shown in the figure comprises a spectrophotometer body 1, a detection cavity is provided on the top of the spectrophotometer body 1, a detection cover 2 is hingedly connected to the top opening of the detection cavity, a U-shaped partition 3 is fixedly connected to the bottom inner wall of the detection cavity, and the opening of the U-shaped partition 3 faces forward, a movable block 4 is arranged on the inner side of the U-shaped partition 3, a pull rod 5 is horizontally fixedly connected to the front end of the movable block 4, and the pull rod 5 is vertically slidable and inserted into the front side of the spectrophotometer body 1, and a support plate 6 is horizontally fixedly connected to the rear side of the movable block 4, and the support plate 6 is horizontally fixedly connected to the rear side of the movable block 4. The bottom of the plate 6 is flush with the bottom of the movable block 4, and two slide rails 7 are arranged in parallel at the bottom of the movable block 4 and the supporting plate 6. The slide rails 7 are fixedly connected to the inner wall of the bottom of the detection chamber, and the slide rails 7 are parallel to the side of the spectrophotometer body 1. The bottom of the movable block 4 and the supporting plate 6 are fixedly connected with two sliders 8, and the two sliders 8 are slidably mounted on the two slide rails 7, respectively. A through hole is opened through the top of the supporting plate 6, and a circular turntable 9 is horizontally arranged on the top of the supporting plate 6, and the top of the turntable 9 is flush with the top of the movable block 4. The bottom of the turntable 9 is fixedly connected with a concentric The support ring 10 is rotatably mounted on the top of the support plate 6. A fixed shaft 11 is vertically fixedly connected to the bottom center of the support plate 6. A one-way gear 12 is fixedly sleeved near the bottom of the fixed shaft 11. A rack 13 is arranged on one side of the one-way gear 12. The rack 13 is fixedly connected to the inner wall of the bottom of the detection chamber, and the teeth on the rack 13 can mesh with the one-way gear 12. Three first detection grooves 14 are evenly arranged on the top of the turntable 9, and the three first detection grooves 14 are distributed in an annular shape. Two first detection grooves 14 are arranged on the top of the movable block 4. Two detection slots 15, and the two second detection slots 15 are distributed front and back, the first detection slot 14 and the second detection slot 15 are both plugged with a cuvette 16, a detection probe 17 is installed on the side of the U-shaped partition 3, a correction component 18 is provided on the rear side of the turntable 9 to correct the turntable 9 after rotation, and a positioning component 19 is provided at the bottom of the movable block 4 to position the movable block 4 after movement, the idling direction of the one-way gear 12 is the same as the forward direction of the movable block 4, and the one-way gear 12 can only drive the turntable 9 to rotate one-third of a circle at a time under the action of the rack 13;

[0032] When testing plant polysaccharides, the reference solution is loaded into a cuvette 16, and the solution to be tested is loaded into four cuvettes 16. When the reference solution and the solution to be tested are evenly loaded, the detection cover 2 is opened, and the cuvette 16 containing the reference solution is inserted into the second detection slot 15 at the front side of the top of the movable block 4, and the four cuvettes 16 containing the solution to be tested are respectively inserted into the remaining second detection slots 15 and the three first detection slots 14. Then, the detection cover 2 is covered and the movable block 4 is pushed by the pull rod 5 so that the cuvette 16 in the second detection slot 15 at the front side of its top is opposite to the detection probe 17. At this time, one of the cuvettes 16 containing the solution to be tested on the turntable 9 can be kept in the same plane with the two cuvettes 16 on the movable block 4. Then, the spectrophotometer body 1 is started. When the detection probe 17 is completed After the detection operation of the reference solution, pull the pull rod 5 to drive the movable block 4 and the turntable 9 to move forward. When the cuvette 16 containing the solution to be tested on the movable block 4 is opposite to the detection probe 17, the positioning assembly 19 can lock the movable block 4. At this time, the detection probe 17 can detect the cuvette 16 containing the solution to be tested on the movable block 4. When the detection is completed, continue to pull the pull rod 5. At this time, the cuvette 16 on the front side of the turntable 9 can move forward. When the cuvette 16 on the front side of the turntable 9 is opposite to the detection probe 17, the detection probe 17 can detect the cuvette 16 on the front side of the turntable 9. At this point, the first group of parallel experiments is completed. In the above process, when the one-way gear 12 contacts the rack 13, the one-way gear 12 can idle, so that the turntable 9 will not rotate.

[0033] When the first group of tests is completed, the pull rod 5 can be pushed to drive the movable block 4 and the turntable 9 to move backward. During this process, when the one-way gear 12 contacts the rack 13, the one-way gear 12 can drive the turntable 9 to rotate through the fixed shaft 11 under the action of the rack 13. After the turntable 9 rotates one-third of a circle, the one-way gear 12 is separated from the rack 13, and the cuvette 16 that has been tested on the top of the turntable 9 can be interchanged with the adjacent cuvette 16 that has not been tested. When the turntable 9 completes its rotation, as the movable block 4 and the turntable 9 continue to move backward, when the turntable 9 contacts the deviation correction component 18, the deviation correction component 18 can fine-tune the turntable 9, so that the next cuvette 16 to be tested can be kept in the same plane with the two cuvettes 16 on the movable block 4, thereby ensuring that the detection probe 17 can be kept facing the cuvette 16 rotated to the front side during the subsequent detection process, thereby ensuring the accuracy of the detection of the detection probe 17;

[0034] When the cuvette 16 on the turntable 9 completes the position exchange, as the pull rod 5 pulls the movable block 4 and the turntable 9 forward, the detection probe 17 can perform the second group of parallel experiments and the subsequent third group of parallel experiments in the above manner. Compared with the existing detection method, the present invention omits the subsequent steps of opening the detection cover 2 to replace the sample to be tested and repeating the calibration of the detection probe 17, thereby greatly improving the detection efficiency.

[0035] like Figures 3 to 5 As shown, the correction component 18 includes an isosceles triangle correction block 181, and the symmetry center line of the correction block 181 is on the same straight line as the axis of the turntable 9, and the rear side of the correction block 181 is horizontally and vertically fixedly connected with a positioning rod 182, and the positioning rod 182 vertically slides through and is inserted in the rear side of the U-shaped partition 3, and the two sides of the positioning rod 182 are symmetrically provided with limit rods 183, and the limit rods 183 are fixedly connected to the correction block 181, and the rear end of the limit rod 183 slides through and is inserted in the rear side of the U-shaped partition 3, and the rear ends of the limit rod 183 and the positioning rod 182 are fixed. A limit block 184, a compression spring 185 is sleeved near the front end of the positioning rod 182, and the two ends of the compression spring 185 are respectively fixedly connected to the correction block 181 and the U-shaped partition 3, and three correction openings 186 matching the correction block 181 are opened on the side of the turntable 9, and the three correction openings 186 are alternately distributed with the three first detection slots 14. When the correction block 181 is plugged into one of the correction slots on the side of the turntable 9, the first detection slot 14 and the two second detection slots 15 facing the correction opening 186 are located on the same straight line, and the three are equidistantly distributed;

[0036] When a set of detection operations is completed, as the movable block 4 and the turntable 9 are pushed backward by the pull rod 5, when the one-way gear 12 contacts the rack 13, as the movable block 4 and the turntable 9 continue to move, the rack 13 can cooperate with the one-way gear 12 to drive the fixed shaft 11 and the turntable 9 to rotate. After the turntable 9 rotates one-third of a circle, the cuvette 16 that has participated in the detection can be rotated to one side, and the undetected cuvette 16 can be rotated to a position opposite to the second detection slot 15. At the same time, the rack 13 is also separated from the one-way gear 12. At this time, as the pull rod 5 is continued to be pushed, the movable block 4 and the turntable 9 can continue to move in the direction close to the correction block 181. When the correction port 186 on the turntable 9 contacts the correction block 181, the correction block 181 can be inserted into the correction port 186, and as the turntable 9 As the turntable 9 continues to move backward, the correction block 181 is gradually squeezed by the turntable 9, and then the correction block 181 can gradually compress the clamping spring 185 and move backward. During this process, the correction block 181 can cooperate with the opposite correction port 186 to drive the turntable 9 to fine-tune and rotate. When the clamping spring 185 is fully compressed, the push rod 5 stops. At this time, the correction block 181 can be tightly inserted in the opposite correction port 186 under the action of the clamping spring 185, and the turntable 9 can also make the cuvette 16 to be detected on the turntable 9 and the two cuvettes 16 on the movable block 4 remain in the same plane under the correction action of the correction block 181 and the correction port 186, thereby ensuring that the detection probe 17 can remain opposite to the cuvette 16 rotated to the front side in the subsequent detection process, thereby ensuring the accuracy of the detection of the detection probe 17.

[0037] like Figure 4 and Figure 7 As shown, the positioning assembly 19 includes a telescopic rod 191, and the telescopic rod 191 is vertically fixedly connected to the bottom inner wall of the detection chamber. A support spring 192 is sleeved on the telescopic rod 191, and the two ends of the support spring 192 are respectively fixedly connected to the surfaces of the telescopic rod 191 near the two ends. The top of the telescopic rod 191 is fixedly connected to a spherical block 193, and three spherical slots 194 matching the spherical block 193 are evenly distributed on the bottom of the movable block 4. The three spherical slots 194 are 4 is distributed front and back, the spherical slot 194 and the spherical block 193 are located on the same motion trajectory, and the distance between two adjacent spherical slots 194 is the same as the distance between the two second detection slots 15. When the spherical block 193 is inserted into the spherical slot 194 at the front side, the detection probe 17 is directly opposite to the side of the cuvette 16 in the second detection slot 15 at the front side, and the maximum elastic force of the compression spring 185 on the correction block 181 is less than the resistance between the spherical block 193 and the spherical slot 194;

[0038] By providing a positioning assembly 19, when the movable block 4 is driven to move along the direction of the guide rail by the pull rod 5, the spherical clamping block 193 at the top of the telescopic rod 191 can slide against the bottom of the movable block 4 under the support of the support spring 192. When the spherical clamping block 193 moves to the position of the spherical clamping groove 194, as the support spring 192 is extended, the spherical clamping block 193 can be inserted into the spherical groove at the current position under the action of the support spring 192, thereby locking the current position of the movable block 4 and the turntable 9. At this time, the detection probe 17 can detect the solution in the cuvette 16 currently facing it;

[0039] And because the maximum elastic force of the compression spring 185 on the deviation-correcting block 181 is smaller than the resistance between the spherical clamping block 193 and the spherical clamping groove 194, when the spherical clamping block 193 is clamped together with the spherical clamping groove 194 at the frontmost side of the bottom of the movable block 4, the spherical clamping block 193 can also cooperate with the current spherical clamping groove 194 to overcome the thrust of the compression spring 185 on the turntable 9 to achieve locking of the position of the movable block 4 and the turntable 9, so that the pull rod 5 can be in the storage state;

[0040] When it is necessary to move the movable block 4 again by the pull rod 5, as the action of the pull rod 5 on the movable block 4 increases, the spherical slot 194 can squeeze the spherical block 193 downward through its smooth spherical surface, so that the telescopic rod 191 and the support spring 192 can retract again until the spherical block 193 is completely removed from the current spherical slot 194, and then the limiting effect of the spherical block 193 on the movable block 4 can be released.

[0041] like Figure 5 and Figure 6 As shown, a deflection slot is provided on the slot wall of the first detection slot 14 away from the rotating disk 9 and on the front slot wall of the second detection slot 15, and a bow-shaped spring piece 20 is fixedly connected to the top slot wall of the deflection slot, both ends of the bow-shaped spring piece 20 are located in the deflection slot, and the middle part of the bow-shaped spring piece 20 is deflected to the outside of the deflection slot;

[0042] By providing a bow-shaped spring piece 20, when the cuvette 16 filled with the detection solution is inserted into the first detection slot 14 or the second detection slot 15, the side of the cuvette 16 near the bottom end can squeeze the bow-shaped spring piece 20, so that the bow-shaped spring piece 20 is deflected into the deflection slot, and the reaction force of the bow-shaped spring piece 20 on the cuvette 16 can cooperate with the slot wall of the first detection slot 14 or the second detection slot to clamp and fix the bottom end of the cuvette 16, thereby ensuring the stability of the installation of the cuvette 16, avoiding the cuvette 16 from tilting due to vibration during the movement of the movable block 4 and the turntable 9, and further ensuring that the side of the cuvette 16 can remain perpendicular to the detection probe 17 during detection, thereby ensuring the accuracy of the detection.

[0043] like Figures 4 to 8 As shown, the rear side of the movable block 4 is designed as an arc surface, and the rear side of the movable block 4 is in contact with the side of the turntable 9. A first groove is provided on the arc surface of the rear side of the movable block 4, and a first magnetic block 21 is installed in the first groove. Three second grooves are evenly provided on the side of the turntable 9, and the three second grooves are opposite to the three first detection grooves 14 one by one. A second magnetic block 22 is installed in the second groove, and the magnetic pole of the second magnetic block 22 is opposite to the magnetic pole of the first magnetic block 21.

[0044] By providing the first magnetic block 21 and the second magnetic block 22, when the turntable 9 rotates one-third of a turn under the action of the rack 13 and the one-way gear 12, the first magnetic block 21 opposite to the second magnetic block 22 can be attracted to each other, thereby locking the state of the turntable 9 after rotation, avoiding the turntable 9 from deflecting due to the vibration generated during movement when the movable block 4 drives the turntable 9 to rotate through the support plate 6, thereby ensuring that the detection probe 17 can more accurately detect the solution in the cuvette 16 on the top of the turntable 9, thereby ensuring the accuracy of the detection.

[0045] like Figure 3 and Figure 5 As shown, two light-proof baffles 23 are vertically fixedly connected to the top of the movable block 4, and the two baffles 23 are respectively located at the rear sides of the two second detection slots 15, and the height and width of the baffles 23 are greater than the height and width of the cuvette 16;

[0046] By providing the baffle 23 , when the detection probe 17 detects the solution in the cuvette 16 directly facing it, the baffle 23 can shield the next cuvette 16 to be detected, thereby ensuring the accuracy of the current detection.

[0047] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit them.

Claims

1. A testing device for extracting plant polysaccharides by enzymatic method, comprising a spectrophotometer body (1), characterized in that: The top of the spectrophotometer body (1) is provided with a detection cavity, the top opening of the detection cavity is hingedly connected to a detection cover (2), the bottom inner wall of the detection cavity is fixedly connected to a U-shaped partition (3), and the opening of the U-shaped partition (3) faces forward, and a movable block (4) is arranged on the inner side of the U-shaped partition (3), the front end of the movable block (4) is horizontally fixedly connected to a pull rod (5), the pull rod (5) vertically slides through and is plugged into the front side of the spectrophotometer body (1), and the rear side of the movable block (4) is horizontally fixedly connected to a support plate (6), and the bottom of the support plate (6) is connected to the movable block (4). The bottom of the movable block (4) and the supporting plate (6) are flush with each other, two slide rails (7) are arranged in parallel at the bottom of the movable block (4) and the supporting plate (6), the slide rails (7) are fixedly connected to the inner wall of the bottom of the detection chamber, and the slide rails (7) are parallel to the side of the spectrophotometer body (1), the bottom of the movable block (4) and the supporting plate (6) are fixedly connected to two sliders (8), and the two sliders (8) are slidably mounted on the two slide rails (7), the top of the supporting plate (6) is penetrated by a through hole, the top of the supporting plate (6) is horizontally arranged with a circular rotating disk (9), and the top of the rotating disk (9) is flush with the top of the movable block (4), The bottom of the rotating disk (9) is fixedly connected to a concentric support ring (10), and the support ring (10) is rotatably mounted on the top of the support plate (6). A fixed shaft (11) is vertically fixedly connected to the center of the bottom circle of the support plate (6). A one-way gear (12) is fixedly sleeved at a position close to the bottom end of the fixed shaft (11). A rack (13) is provided on one side of the one-way gear (12). The rack (13) is fixedly connected to the inner wall of the bottom of the detection chamber, and the teeth on the rack (13) can mesh with the one-way gear (12). The top of the rotating disk (9) is evenly provided with three first detection A groove (14) is formed on the top of the movable block (4), and the three first detection grooves (14) are arranged in a ring shape. Two second detection grooves (15) are arranged on the top of the movable block (4), and the two second detection grooves (15) are arranged in a front-to-back manner. A cuvette (16) is inserted into each of the first detection groove (14) and the second detection groove (15). A detection probe (17) is installed on the side of the U-shaped partition (3). A correction component (18) is arranged on the rear side of the turntable (9) for correcting the turntable (9) after rotation. A positioning component (19) is arranged on the bottom of the movable block (4) for positioning the movable block (4) after movement. The deflection correction component (18) comprises an isosceles triangle deflection correction block (181), wherein the symmetry center line of the deflection correction block (181) and the axis center of the turntable (9) are located on the same straight line, a positioning rod (182) is fixedly connected to the rear side of the deflection correction block (181) in a horizontal and vertical manner, the positioning rod (182) is vertically slidably inserted through the rear side of the U-shaped partition (3), and limiting rods (183) are symmetrically arranged on both sides of the positioning rod (182), the limiting rods (183) are fixedly connected to the deflection correction block (181), and the rear end of the limiting rod (183) slides The rotating disk (9) is inserted through the rear side of the U-shaped partition (3), and the rear ends of the limiting rod (183) and the positioning rod (182) are fixed with a limiting block (184), the positioning rod (182) is sleeved with a compression spring (185) near the front end, and the two ends of the compression spring (185) are respectively fixedly connected to the correction block (181) and the U-shaped partition (3), and the side of the rotating disk (9) is provided with three correction openings (186) matching the correction block (181), and the three correction openings (186) are alternately distributed with the three first detection slots (14); When the deflection correction block (181) is plugged into one of the deflection correction slots on the side of the rotating disk (9), the first detection slot (14) and the two second detection slots (15) facing the deflection correction slot (186) are located on the same straight line, and the three are equidistantly distributed.

2. The enzymatic extraction plant polysaccharide testing device according to claim 1, characterized in that: The positioning assembly (19) comprises a telescopic rod (191), the telescopic rod (191) being vertically fixedly connected to the bottom inner wall of the detection chamber, a support spring (192) being sleeved on the telescopic rod (191), and two ends of the support spring (192) being respectively fixedly connected to surfaces of the telescopic rod (191) near the two ends, a spherical clamping block (193) being fixedly connected to the top end of the telescopic rod (191), and three spherical clamping blocks (193) being evenly distributed on the bottom of the movable block (4) The three spherical card slots (194) are distributed front to back, the spherical card slots (194) and the spherical card block (193) are located on the same motion trajectory, and the distance between two adjacent spherical card slots (194) is the same as the distance between two second detection slots (15). When the spherical card block (193) is inserted into the frontmost spherical card slot (194), the detection probe (17) faces the side of the cuvette (16) in the frontmost second detection slot (15).

3. The enzymatic extraction plant polysaccharide testing device according to claim 1, characterized in that: A deflection slot is provided on the slot wall of the first detection slot (14) on the side away from the rotating disk (9) and on the front slot wall of the second detection slot (15), a bow-shaped spring piece (20) is fixedly connected to the top slot wall of the deflection slot, both ends of the bow-shaped spring piece (20) are located in the deflection slot, and the middle part of the bow-shaped spring piece (20) is deflected to the outside of the deflection slot.

4. The enzymatic extraction test device for plant polysaccharides according to claim 3, characterized in that: The rear side of the movable block (4) is designed as an arc surface, and the rear side of the movable block (4) is in contact with the side of the turntable (9). A first groove is provided on the arc surface of the rear side of the movable block (4), and a first magnetic block (21) is installed in the first groove. Three second grooves are evenly provided on the side of the turntable (9), and the three second grooves are opposite to the three first detection grooves (14) one by one. A second magnetic block (22) is installed in the second groove, and the magnetic poles of the second magnetic block (22) and the first magnetic block (21) are opposite.

5. The enzymatic extraction test device for plant polysaccharides according to claim 4, characterized in that: The idling direction of the one-way gear (12) is the same as the forward direction of the movable block (4), and the one-way gear (12) can only drive the rotating disk (9) to rotate one-third of a circle at a time under the action of the rack (13).

6. The enzymatic extraction plant polysaccharide testing device according to claim 5, characterized in that: Two light-proof baffles (23) are vertically fixedly connected to the top of the movable block (4), the two baffles (23) are respectively located at the rear sides of the two second detection slots (15), and the height and width of the baffles (23) are greater than the height and width of the cuvette (16).

7. The enzymatic extraction plant polysaccharide testing device according to claim 2, characterized in that: The maximum elastic force of the compression spring (185) on the deviation-correcting block (181) is smaller than the resistance between the spherical clamping block (193) and the spherical clamping groove (194).

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