Embryonic stem cell bionic culture device

By designing a biomimetic culture device for automatic fixation, rotation and heating, the problems of cumbersome fixation and uneven heating in the prior art are solved, efficient cell culture and temperature control are achieved, and cell activity is ensured.

CN118995420BActive Publication Date: 2024-12-27SHENZHEN ZHIYIN CELL BIOTECHNOLOGY CO LTD
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Patent Information

Application Number
CN202411501597.8
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2024-10-25
Publication Date
2024-12-27
Estimated Expiration
2044-10-25

AI Technical Summary

Technical Problem

The existing embryonic stem cell culture devices are complicated during fixation and rotation, affecting efficiency, and are not uniform enough to maintain cell activity and appropriate temperature.

Method used

A bionic culture device for embryonic stem cells including a rotating mechanism, a heating mechanism and a sealing mechanism is designed to automatically fix and rotate the culture dish through a threaded rod and a motor, and fully heated with a heating plate and a moving heating plate, and pass through an elastic rod. and extrusion rod achieve sealing.

Benefits of technology

The fixation process of the culture dish is simplified, the rotation efficiency is improved, the stem cells are always active, and the appropriate temperature is maintained by uniform heating, which improves the efficiency and effectiveness of cell culture.

✦ Generated by Eureka AI based on patent content.

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Abstract

The present invention relates to the field of bionic culture technology, and discloses an embryonic stem cell bionic culture device, comprising a workbench, the surface of the workbench is fixedly connected with a slide rail, the end of the workbench is fixedly connected with a motor, the output end of the motor is fixedly connected with a threaded rod, and the bottom of the workbench is fixedly connected with a support frame. The present invention first puts a culture dish into the top of a threaded placement rack, then starts the motor to drive the threaded rod to rotate, when the threaded rod rotates, the moving ring moves toward the direction of the culture dish, when the moving ring moves, the sliding plate is driven to move toward the direction of the culture dish, when the sliding plate moves, the push rod is pushed to move in a direction close to each other, when the push rod moves, the passive rod is pushed to rotate, when the passive rod rotates, the threaded placement rack is moved downward, when the threaded placement rack moves downward, the culture dish is driven to move downward, when the sliding plate moves, the moving plate is driven to move in a direction close to each other.
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Description

Technical Field

[0001] The invention relates to the technical field of bionic culture equipment, in particular to a bionic culture device for embryonic stem cells. Background Art

[0002] Embryonic stem cells are highly pluripotent, which means they can differentiate into any type of cell in the body. This property makes embryonic stem cells have great potential in medical research and clinical applications.

[0003] In the prior art, when culturing embryonic stem cells, the stem cells are first placed in a culture dish, and then the culture dish is rotated by a rotating device to keep the stem cells in the culture dish active. The stem cells in the culture dish are then heated to keep them warm. When rotating the culture dish, workers first adjust the rotating device to fix the culture dish. However, the process of adjusting the rotating device to fix it is relatively cumbersome and reduces the cultivation efficiency. Summary of the invention

[0004] The purpose of the present invention is to provide an embryonic stem cell bionic culture device to solve the problems raised in the above background technology.

[0005] In order to solve the above technical problems, the present invention is achieved through the following technical solutions:

[0006] The present invention is an embryonic stem cell bionic culture device, comprising a workbench, a slide rail is fixedly connected to the surface of the workbench, a motor is fixedly connected to the end of the workbench, a threaded rod is fixedly connected to the output end of the motor, a support frame is fixedly connected to the bottom of the workbench, and further comprising:

[0007] A rotating mechanism, the rotating mechanism comprising a sliding rod, a surface of the sliding rod being fixedly connected to a buffer elastic rod, and an end of the buffer elastic rod away from the sliding rod being fixedly connected to a contact plate;

[0008] A heating mechanism, the heating mechanism comprising a fixed heating plate, an inner wall of the fixed heating plate is fixedly connected to a telescopic plate, and an end of the telescopic plate away from the fixed heating plate is fixedly connected to a movable heating plate;

[0009] The sealing mechanism comprises a sliding block, a surface of which is rotatably connected to an extrusion rod, and an end of the extrusion rod away from the sliding block is fixedly connected to an extension elastic rod.

[0010] Furthermore, the number of the slide rails is set to four, and they are divided into two groups of two, and the four slide rails are symmetrically arranged on the surface of the workbench. The number of the support frames is set to four, and the four support frames are symmetrically arranged at the bottom of the workbench. The surface of the workbench is provided with a limiting groove.

[0011] Further, the rotating mechanism includes a sliding plate, a moving ring is fixedly connected to the bottom of the sliding plate, moving plates are fixedly connected to both sides of the sliding plate, a push rod is rotatably connected to the surface of the sliding plate, an end of the push rod away from the sliding plate is rotatably connected to a passive rod, an inner wall of the passive rod is threadedly connected to a threaded placement rack, a culture dish is arranged on the top of the threaded placement rack, a support block is fixedly connected to the surface of the sliding plate, a rotating motor is fixedly connected to the surface of the support block, and a rotating shaft is fixedly connected to the output end of the rotating motor.

[0012] Furthermore, a fixing ring is fixedly connected to the top of the support block, a rotating groove is provided on the inner wall of the fixing ring, a rotating disk is rotatably connected to the inner wall of the rotating groove, a guide rail is fixedly connected to the surface of the rotating disk, a lower pressure groove is provided on the surface of the rotating disk, a lower pressure elastic rod is fixedly connected to the inner wall of the lower pressure groove, an end of the lower pressure elastic rod away from the lower pressure groove is fixedly connected to the lower pressure disk, a pulling rod is rotatably connected to the surface of the lower pressure disk, an end of the pulling rod away from the lower pressure disk is rotatably connected to a sliding rod, and shrinkage rings are fixedly connected to both sides of the sliding rod.

[0013] Furthermore, the inner wall of the sliding plate is slidably connected to the surface of the sliding rail, the surface of the threaded rod is threadedly connected to the inner wall of the movable ring, the surface of the guide rail is slidably connected to the bottom of the sliding rod, the end of the rotating shaft away from the rotating motor is fixedly connected to the surface of the rotating disk, and the number of the contact plates is four, and the four contact plates are symmetrically arranged with the lower pressure plate as the center.

[0014] Furthermore, the heating mechanism includes a right-angle plate, the inner surface of the right-angle plate is provided with a rising groove, the inner wall of the rising groove is slidably connected with a slider, the surface of the slider is fixedly connected with a driven column, the surface of the driven column is rotatably connected with a rising rod, the end of the rising rod away from the driven column is rotatably connected to a sliding frame, the end of the sliding frame is fixedly connected to a limiting elastic rod, the surface of the sliding frame is fixedly connected with a driving plate, the surface of the slider is fixedly connected with a rising frame, the end of the rising frame away from the slider is fixedly connected with a fixed heating plate, the surface of the movable heating plate is rotatably connected with an expansion rod, and the surface of the rising frame is fixedly connected with a supporting elastic rod.

[0015] Furthermore, one end of the movable plate away from the sliding plate is fixedly connected to the surface of the sliding frame, the bottom of the right-angle plate is fixedly connected to the surface of the workbench, one end of the expansion rod away from the movable heating plate is rotatably connected to the inner wall and top wall of the right-angle plate, and there are four rising rods, which are divided into two groups of two in each group. The four rising rods are symmetrically arranged with the culture dish as the center.

[0016] Furthermore, the sealing mechanism includes a fixed frame, the end of the fixed frame is fixedly connected with a slide plate, the end of the extended elastic rod is fixedly connected with a fixed plate, the two sides of the sliding block are fixedly connected with pulling plates, the end of the pulling plate away from the sliding block is fixedly connected with a sealing box, the surface of the sealing box is provided with a fixing groove, and the end of the sealing box is fixedly connected with a merging plate.

[0017] Furthermore, one end of the driving plate away from the sliding frame is fixedly connected to the inner wall of the sealing box, a merging groove is provided at the end of the sealing box, one end of the extending elastic rod away from the fixed plate is fixedly connected to the inner wall of the slide plate, and one end of the fixed frame away from the slide plate is fixedly connected to the bottom of the workbench.

[0018] The present invention has the following beneficial effects:

[0019] The present invention provides a rotating mechanism. First, a culture dish is placed on the top of the threaded placement rack. Then, a motor is started to drive the threaded rod to rotate. When the threaded rod rotates, the moving ring moves toward the culture dish. When the moving ring moves, the sliding plate is driven to move toward the culture dish. When the sliding plate moves, the push rod is pushed to move in a direction close to each other. When the push rod moves, the passive rod is driven to rotate. When the passive rod rotates, the threaded placement rack moves downward. When the threaded placement rack moves downward, the culture dish is driven downward. When the sliding plate moves, the moving plate is driven to move in a direction close to each other. When the sliding plate moves, the supporting block is driven to move in a direction close to each other. The supporting block drives the fixed ring to move in a direction close to each other. The fixed ring drives the rotating disk to move in a direction close to each other. The lower pressing plate is connected to the When the two ends of the culture dish are touched, the elastic rod is pressed backward, and when the lower pressure plate moves backward, it drives the pulling rod to move downward, and when the pulling rod moves, the sliding rod is pulled to slide on the surface of the guide rail in the direction of approaching each other, and when the sliding rod moves, it drives the shrinkage ring to move in the direction of approaching each other and shrink, and when the sliding rod moves, it pushes the buffering elastic rod to move in the direction of approaching each other, and when the buffering elastic rod moves, it pushes the contact plate to move in the direction of approaching each other, and then the rotating motor is started to drive the rotating shaft to rotate, and when the rotating shaft rotates, it drives the rotating disk to rotate on the inner wall of the rotating groove, effectively fixing and rotating the culture dish, and more effectively fixing culture dishes with different diameters, saving the workers the tedious steps of fixing the culture dish, and keeping the stem cells inside the culture dish active at all times through rotation.

[0020] The heating mechanism of the present invention is provided with a heating mechanism, when the movable plate moves in the direction approaching each other, the sliding frame is driven to move in the direction approaching each other, the sliding frame is pressed when the limiting elastic rod is moved, and when the sliding frame moves, the driving plate is driven to move in the direction approaching each other, and when the sliding frame moves, the lifting rod is pushed to move in the direction approaching each other, and when the sliding frame is subjected to a thrust, the driven column is pushed to move upward, and when the driven column moves upward, the slider is driven to slide upward on the inner wall of the lifting groove, and when the slider slides upward, the lifting frame is driven to move upward, and the lifting frame drives the fixed heating plate to move upward, and the fixed heating plate drives the lifting and shrinking plate to move upward, and when the lifting and shrinking plate moves upward, the movable heating plate is driven to move upward, and when the movable heating plate moves upward, the expansion rod is pushed upward, and when the movable heating plate moves upward, the expansion rod is subjected to a thrust and expands to both sides, and when the expansion rod expands, the movable heating plate is driven to expand in the direction away from each other, and when the movable heating plate expands, the telescopic plate is driven to extend in the direction away from each other, so that the culture dish is effectively fully heated by the fixed heating plate and the movable heating plate, so that the stem cells inside the culture dish are kept at an appropriate temperature.

[0021] The present invention provides a sealing mechanism. When the driving plate moves in the direction of approaching each other, it drives the sealing box to move in the direction of approaching each other. When the sealing box moves, it drives the pulling plate to move in the direction of approaching each other. When the pulling plate moves, it drives the sliding block to move on the inner wall of the fixed frame in the direction of approaching each other. When the sliding block moves, it pushes the extrusion rod to move in the direction of approaching each other. When the extrusion rod is subjected to thrust, it pushes the extending elastic rod to extend in the direction of approaching each other. When the extending elastic rod is extended, it pushes the fixed plate to move in the direction of approaching each other. When the sealing box pushes the pulling plate to move, it drives the merging plate to move in the direction of the merging groove and closes with the inner wall of the merging groove to make it sealed. When the merging plate merges with the inner wall of the merging groove, the fixing plate extends to the inner wall of the fixing groove to be clamped and fixed, thereby effectively sealing the stem cells. Compared with opening and closing doors, it is more convenient.

[0022] Of course, any product implementing the present invention does not necessarily need to achieve all of the advantages described above at the same time. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following briefly introduces the drawings required for describing the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0024] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0025] Figure 2 It is a schematic diagram of the overall cross-sectional structure of the present invention;

[0026] Figure 3 It is a schematic diagram of the overall structure of the rotating mechanism of the present invention;

[0027] Figure 4 This is a schematic diagram of the structure of the lower pressure plate of the present invention;

[0028] Figure 5 It is a schematic diagram of the overall structure of the heating mechanism of the present invention;

[0029] Figure 6 This is a schematic diagram of the structure of the rising frame of the present invention;

[0030] Figure 7 For the present invention Figure 6 A schematic diagram of the enlarged structure of part A;

[0031] Figure 8 It is a schematic diagram of the overall structure of the sealing mechanism of the present invention;

[0032] Fig. 9 It is a schematic diagram of the fixing plate structure of the present invention.

[0033] In the accompanying drawings, the components represented by the reference numerals are listed as follows:

[0034] In the figure: 1, workbench; 2, slide rail; 3, motor; 4, threaded rod; 5, support frame; 10, rotating mechanism; 11, sliding plate; 12, moving ring; 13, moving plate; 14, push rod; 15, passive rod; 16, threaded placement frame; 17, culture dish; 18, support block; 19, rotating motor; 20, rotating shaft; 21, fixed ring; 22, rotating disk; 23, guide rail; 24, pressing elastic rod; 25, pressing disk; 26, pulling rod; 27, sliding rod; 28, shrinking ring; 29, buffer elastic rod; 30, contact Plate; 40, heating mechanism; 41, right-angle plate; 42, slider; 43, driven column; 44, rising rod; 45, sliding frame; 46, limiting elastic rod; 47, driving plate; 48, rising frame; 49, fixed heating plate; 50, telescopic plate; 51, movable heating plate; 52, expansion rod; 53, supporting elastic rod; 60, sealing mechanism; 61, fixed frame; 62, slide plate; 63, sliding block; 64, extrusion rod; 65, extension elastic rod; 66, fixed plate; 67, pulling plate; 68, sealing box; 69, merging plate. DETAILED DESCRIPTION

[0035] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0036] See also Figure 1 - Fig. 9 As shown, the present invention is an embryonic stem cell bionic culture device, comprising a workbench 1, a slide rail 2 is fixedly connected to the surface of the workbench 1, a motor 3 is fixedly connected to the end of the workbench 1, a threaded rod 4 is fixedly connected to the output end of the motor 3, and then the motor 3 is started to drive the threaded rod 4 to rotate, and a support frame 5 is fixedly connected to the bottom of the workbench 1, and further comprising;

[0037] The rotating mechanism 10 includes a sliding rod 27. When the pulling rod 26 moves, the sliding rod 27 slides on the surface of the guide rail 23 in a direction of approaching each other. A buffer elastic rod 29 is fixedly connected to the surface of the sliding rod 27. When the sliding rod 27 moves, the buffer elastic rod 29 is pushed to move in a direction of approaching each other. One end of the buffer elastic rod 29 away from the sliding rod 27 is fixedly connected to a contact plate 30. When the buffer elastic rod 29 moves, the contact plate 30 is pushed to move in a direction of approaching each other.

[0038] The heating mechanism 40 includes a fixed heating plate 49. The rising frame 48 drives the fixed heating plate 49 to move upward. The inner wall of the fixed heating plate 49 is fixedly connected with a telescopic plate 50. The fixed heating plate 49 drives the telescopic plate 50 to move upward. When the mobile heating plate 51 expands, the telescopic plate 50 is driven to extend in a direction away from each other. One end of the telescopic plate 50 away from the fixed heating plate 49 is fixedly connected with the mobile heating plate 51. When the expansion rod 52 expands, the mobile heating plate 51 is driven to expand in a direction away from each other.

[0039] The sealing mechanism 60 includes a sliding block 63, the surface of which is rotatably connected to a squeezing rod 64. When the sliding block 63 moves, it pushes the squeezing rod 64 to move in a direction approaching each other. One end of the squeezing rod 64 away from the sliding block 63 is fixedly connected to an extension elastic rod 65. When the squeezing rod 64 is subjected to a thrust, it pushes the extension elastic rod 65 to extend in a direction approaching each other.

[0040] There are four slide rails 2, which are divided into two groups of two. The four slide rails 2 are symmetrically arranged on the surface of the workbench 1. There are four support frames 5, which are symmetrically arranged at the bottom of the workbench 1. A limiting groove is opened on the surface of the workbench 1.

[0041] The rotating mechanism 10 includes a sliding plate 11, and a moving ring 12 is fixedly connected to the bottom of the sliding plate 11. When the threaded rod 4 rotates, the moving ring 12 moves toward the culture dish 17. Moving plates 13 are fixedly connected to both sides of the sliding plate 11. When the sliding plate 11 moves, the moving plates 13 are driven to move in a direction close to each other. A push rod 14 is rotatably connected to the surface of the sliding plate 11. When the sliding plate 11 moves, the push rod 14 is pushed to move in a direction close to each other. The end of the push rod 14 away from the sliding plate 11 is rotatably connected to a passive rod 15. When the push rod 14 moves, it pushes the passive rod 15 5 rotates, the inner wall of the passive rod 15 is threadedly connected with a threaded placement rack 16. When the passive rod 15 rotates, the threaded placement rack 16 moves downward. A culture dish 17 is arranged on the top of the threaded placement rack 16. First, the culture dish 17 is placed on the top of the threaded placement rack 16. The surface of the sliding plate 11 is fixedly connected with a support block 18. When the sliding plate 11 moves, the support block 18 is driven to move in a direction of approaching each other. The surface of the support block 18 is fixedly connected with a rotating motor 19. The output end of the rotating motor 19 is fixedly connected with a rotating shaft 20. Then, the rotating motor 19 is started to drive the rotating shaft 20 to rotate.

[0042] A fixing ring 21 is fixedly connected to the top of the support block 18, and the support block 18 drives the fixing ring 21 to move in a direction approaching each other. A rotating groove is provided on the inner wall of the fixing ring 21, and a rotating disk 22 is rotatably connected to the inner wall of the rotating groove. When the rotating shaft 20 rotates, the rotating disk 22 is driven to rotate on the inner wall of the rotating groove. The fixing ring 21 drives the rotating disk 22 to move in a direction approaching each other, and a guide rail 23 is fixedly connected to the surface of the rotating disk 22. A downward pressing groove is provided on the surface of the rotating disk 22, and a downward pressing elastic rod 24 is fixedly connected to the inner wall of the downward pressing groove. A downward pressing disk 25 is fixedly connected to the end of the downward pressing elastic rod 24 away from the downward pressing groove. When the downward pressing disk 25 contacts The two ends of the culture dish 17 squeeze the elastic rod 24 backward, and the surface of the lower pressure plate 25 is rotatably connected with a pulling rod 26. When the lower pressure plate 25 moves backward, it drives the pulling rod 26 to move downward. The end of the pulling rod 26 away from the lower pressure plate 25 is rotatably connected with a sliding rod 27, and the two sides of the sliding rod 27 are fixedly connected with shrinkage rings 28. When the sliding rod 27 moves, it drives the shrinkage rings 28 to move in a direction close to each other and shrink, thereby effectively fixing and rotating the culture dish, and more effectively fixing culture dishes with different diameters, saving the workers the tedious steps of fixing the culture dish, and keeping the stem cells inside the culture dish 17 active at all times through rotation.

[0043] The inner wall of the sliding plate 11 is slidably connected to the surface of the slide rail 2. When the moving ring 12 moves, the sliding plate 11 is driven to move toward the culture dish 17. When the threaded placement rack 16 moves downward, the culture dish 17 is driven downward. The surface of the threaded rod 4 is threadedly connected to the inner wall of the moving ring 12. The surface of the guide rail 23 is slidably connected to the bottom of the sliding rod 27. The end of the rotating shaft 20 away from the rotating motor 19 is fixedly connected to the surface of the rotating disk 22. There are four contact plates 30, and the four contact plates 30 are symmetrically arranged with the lower pressure plate 25 as the center.

[0044] The heating mechanism 40 includes a right-angle plate 41. When the driven column 43 moves upward, the slider 42 is driven to slide upward on the inner wall of the rising groove. The inner surface of the right-angle plate 41 is provided with a rising groove. The inner wall of the rising groove is slidably connected with the slider 42. The surface of the slider 42 is fixedly connected with the driven column 43. When the sliding frame 45 is subjected to a thrust and pushes the driven column 43 to move upward, the surface of the driven column 43 is rotatably connected with the rising rod 44. The end of the rising rod 44 away from the driven column 43 is rotatably connected with the sliding frame 45. When the sliding frame 45 moves, it pushes the rising rod 44 to move in a direction close to each other. When the moving plate 13 moves in a direction close to each other, it drives the sliding frame 45 to move in a direction close to each other. The end of the sliding frame 45 is fixedly connected with The limiting elastic rod 46 squeezes the limiting elastic rod 46 when the sliding frame 45 moves. A driving plate 47 is fixedly connected to the surface of the sliding frame 45. When the sliding frame 45 moves, the driving plate 47 is driven to move towards each other. A rising frame 48 is fixedly connected to the surface of the slider 42. When the slider 42 slides upward, the rising frame 48 is driven to move upward. The end of the rising frame 48 away from the slider 42 is fixedly connected to a fixed heating plate 49. The surface of the mobile heating plate 51 is rotatably connected to an expansion rod 52. The surface of the rising frame 48 is fixedly connected to a supporting elastic rod 53. The culture dish 17 is effectively fully heated by the fixed heating plate 49 and the mobile heating plate 51, so that the stem cells inside the culture dish 17 are maintained at an appropriate temperature.

[0045] One end of the movable plate 13 away from the sliding plate 11 is fixedly connected to the surface of the sliding frame 45, the bottom of the right-angle plate 41 is fixedly connected to the surface of the workbench 1, and one end of the expansion rod 52 away from the movable heating plate 51 is rotatably connected to the inner wall and top wall of the right-angle plate 41. When the movable heating plate 51 moves upward, it pushes the expansion rod 52 to move upward. When the expansion rod 52 is pushed, it expands to both sides. There are four rising rods 44, which are divided into two groups of two each. The four rising rods 44 are symmetrically arranged with the culture dish 17 as the center.

[0046] The sealing mechanism 60 includes a fixed frame 61, and a slide plate 62 is fixedly connected to the end of the fixed frame 61, and a fixed plate 66 is fixedly connected to the end of the extended elastic rod 65. Pulling plates 67 are fixedly connected to both sides of the sliding block 63. When the sealing box 68 moves, the pulling plate 67 is driven to move in a direction close to each other. The end of the pulling plate 67 away from the sliding block 63 is fixedly connected to the sealing box 68. A fixing groove is provided on the surface of the sealing box 68. A merging plate 69 is fixedly connected to the end of the sealing box 68. When the sealing box 68 pushes the pulling plate 67 to move, it drives the merging plate 69 to move in the direction of the merging groove and closes with the inner wall of the merging groove to make it sealed. When the merging plate 69 merges with the inner wall of the merging groove, the fixed plate 66 extends to the inner wall of the fixed groove to be clamped and fixed, which effectively seals the stem cell, which is more convenient than opening and closing the door.

[0047] One end of the driving plate 47 away from the sliding frame 45 is fixedly connected to the inner wall of the sealing box 68, and a merging groove is provided at the end of the sealing box 68. When the driving plate 47 moves in the direction of approaching each other, it drives the sealing box 68 to move in the direction of approaching each other. The end of the extended elastic rod 65 away from the fixed plate 66 is fixedly connected to the inner wall of the slide plate 62. When the extended elastic rod 65 is extended, it pushes the fixed plate 66 to move in the direction of approaching each other. The end of the fixed frame 61 away from the slide plate 62 is fixedly connected to the bottom of the workbench 1. When the pulling plate 67 moves, it drives the sliding block 63 to move in the direction of approaching each other on the inner wall of the fixed frame 61.

[0048] When in use, first place the culture dish 17 on the top of the threaded placement rack 16, then start the motor 3 to drive the threaded rod 4 to rotate, and when the threaded rod 4 rotates, the moving ring 12 moves toward the culture dish 17, and when the moving ring 12 moves, it drives the sliding plate 11 to move toward the culture dish 17, and when the sliding plate 11 moves, it pushes the push rod 14 to move in a direction close to each other, and when the push rod 14 moves, it drives the passive rod 15 to rotate, and when the passive rod 15 rotates, the threaded placement rack 16 moves downward, and when the threaded placement rack 16 moves downward, it drives the culture dish 17 to move downward, and when the sliding plate 11 moves, it drives the moving plate 13 to move in a direction close to each other, and when the sliding plate 11 moves, it drives the supporting block 18 to move in a direction close to each other. , the support block 18 drives the fixed ring 21 to move in the direction of approaching each other, and the fixed ring 21 drives the rotating disk 22 to move in the direction of approaching each other. When the lower pressing plate 25 contacts the two ends of the culture dish 17, it squeezes the lower pressing elastic rod 24 backward. When the lower pressing plate 25 moves backward, it drives the pulling rod 26 to move downward. When the pulling rod 26 moves, the sliding rod 27 slides on the surface of the guide rail 23 in the direction of approaching each other. When the sliding rod 27 moves, it drives the shrinking ring 28 to move in the direction of approaching each other and shrinks. When the sliding rod 27 moves, it pushes the buffer elastic rod 29 to move in the direction of approaching each other. When the buffer elastic rod 29 moves, it pushes the contact plate 30 to move in the direction of approaching each other, and then the rotating motor 19 is started to drive the rotating shaft 20 to rotate. When the rotating shaft 20 is rotated, When the moving shaft 20 rotates, the rotating disk 22 is driven to rotate on the inner wall of the rotating groove. When the moving plate 13 moves in the direction of approaching each other, the sliding frame 45 is driven to move in the direction of approaching each other. When the sliding frame 45 moves, it drives the driving plate 47 to move in the direction of approaching each other. When the sliding frame 45 moves, it pushes the rising rod 44 to move in the direction of approaching each other. When the sliding frame 45 is pushed, it pushes the driven column 43 to move upward. When the driven column 43 moves upward, it drives the slider 42 to slide upward on the inner wall of the rising groove. When the slider 42 slides upward, it drives the rising frame 48 to move upward. The rising frame 48 drives the fixed heating plate 49 to move upward, and the fixed heating plate 49 drives the telescopic plate 5 0 moves upward, when the telescopic plate 50 moves upward, it drives the mobile heating plate 51 to move upward, when the mobile heating plate 51 moves upward, it pushes the expansion rod 52 to move upward, when the expansion rod 52 is pushed and expands to both sides, when the expansion rod 52 expands, it drives the mobile heating plate 51 to expand in the direction away from each other, when the mobile heating plate 51 expands, it drives the telescopic plate 50 to extend in the direction away from each other, when the driving plate 47 moves in the direction of approaching each other, it drives the sealing box 68 to move in the direction of approaching each other, when the sealing box 68 moves, it drives the pulling plate 67 to move in the direction of approaching each other, when the pulling plate 67 moves, it drives the sliding block 63 to move in the direction of approaching each other on the inner wall of the fixed frame 61,When the sliding block 63 moves, it pushes the extrusion rod 64 to move in the direction of approaching each other. When the extrusion rod 64 is pushed, it pushes the extension elastic rod 65 to extend in the direction of approaching each other. When the extension elastic rod 65 extends, it pushes the fixing plate 66 to move in the direction of approaching each other. When the sealing box 68 pushes the pulling plate 67 to move, it drives the merging plate 69 to move in the direction of the merging groove and closes with the inner wall of the merging groove to make it sealed. When the merging plate 69 merges with the inner wall of the merging groove, the fixing plate 66 extends to the inner wall of the fixing groove to be clamped and fixed.

[0049] The preferred embodiments of the present invention disclosed above are only used to help illustrate the present invention. The preferred embodiments do not describe all the details in detail, nor do they limit the invention to the specific implementation methods described. Obviously, many modifications and changes can be made according to the content of this specification. This specification selects and specifically describes these embodiments in order to better explain the principles and practical applications of the present invention, so that those skilled in the art can understand and use the present invention well. The present invention is limited only by the claims and their full scope and equivalents.

Claims

1. An embryonic stem cell bionic culture device, comprising a workbench (1), a slide rail (2) fixedly connected to the surface of the workbench (1), a motor (3) fixedly connected to the end of the workbench (1), a threaded rod (4) fixedly connected to the output end of the motor (3), a support frame (5) fixedly connected to the bottom of the workbench (1), the slide rails (2) being provided in four numbers and divided into two groups of two each, the four slide rails (2) being symmetrically arranged on the surface of the workbench (1), the support frames (5) being provided in four numbers, the four support frames (5) being symmetrically arranged at the bottom of the workbench (1), and the surface of the workbench (1) being provided with a limiting groove, characterized in that: Also includes; A rotating mechanism (10), the rotating mechanism (10) comprising a sliding rod (27) and a sliding plate (11), the surface of the sliding rod (27) being fixedly connected to a buffer elastic rod (29), one end of the buffer elastic rod (29) away from the sliding rod (27) being fixedly connected to a contact plate (30), the bottom of the sliding plate (11) being fixedly connected to a moving ring (12), both sides of the sliding plate (11) being fixedly connected to moving plates (13), the surface of the sliding plate (11) being rotatably connected to a push rod (14), the push rod (14) being away from the sliding plate One end of the sliding plate (11) is rotatably connected to a passive rod (15), the inner wall of the passive rod (15) is threadedly connected to a threaded placement rack (16), a culture dish (17) is arranged on the top of the threaded placement rack (16), the surface of the sliding plate (11) is fixedly connected to a support block (18), the surface of the support block (18) is fixedly connected to a rotating motor (19), the output end of the rotating motor (19) is fixedly connected to a rotating shaft (20), the top of the support block (18) is fixedly connected to a fixing ring (21), the fixing ring (21) A rotating groove is provided on the inner wall, a rotating disk (22) is rotatably connected to the inner wall of the rotating groove, a guide rail (23) is fixedly connected to the surface of the rotating disk (22), a lower pressing groove is provided on the surface of the rotating disk (22), a lower pressing elastic rod (24) is fixedly connected to the inner wall of the lower pressing groove, an end of the lower pressing elastic rod (24) away from the lower pressing groove is fixedly connected to the lower pressing disk (25), a pulling rod (26) is rotatably connected to the surface of the lower pressing disk (25), and an end of the pulling rod (26) away from the lower pressing disk (25) is rotatably connected to a sliding rod (27) , shrink rings (28) are fixedly connected to both sides of the slide rod (27), the inner wall of the slide plate (11) is slidably connected to the surface of the slide rail (2), the surface of the threaded rod (4) is threadedly connected to the inner wall of the movable ring (12), the surface of the guide rail (23) is slidably connected to the bottom of the slide rod (27), the end of the rotating shaft (20) away from the rotating motor (19) is fixedly connected to the surface of the rotating disk (22), and the number of the contact plates (30) is four, and the four contact plates (30) are symmetrically arranged with the lower pressure plate (25) as the center; A heating mechanism (40), the heating mechanism (40) comprising a fixed heating plate (49) and a right-angle plate (41), the inner wall of the fixed heating plate (49) being fixedly connected to a telescopic plate (50), one end of the telescopic plate (50) away from the fixed heating plate (49) being fixedly connected to a movable heating plate (51), the inner surface of the right-angle plate (41) being provided with a rising groove, the inner wall of the rising groove being slidably connected to a slider (42), the surface of the slider (42) being fixedly connected to a driven column (43), the surface of the driven column (43) being rotatably connected to a rising rod (44), the upper One end of the lifting rod (44) away from the driven column (43) is rotatably connected to a sliding frame (45), the end of the sliding frame (45) is fixedly connected to a limiting elastic rod (46), the surface of the sliding frame (45) is fixedly connected to a driving plate (47), the surface of the sliding block (42) is fixedly connected to a lifting frame (48), one end of the lifting frame (48) away from the sliding block (42) is fixedly connected to a fixed heating plate (49), the surface of the movable heating plate (51) is rotatably connected to an expansion rod (52), and the surface of the lifting frame (48) is fixedly connected to a supporting elastic rod (53); The sealing mechanism (60) comprises a sliding block (63), the surface of the sliding block (63) being rotatably connected to an extrusion rod (64), and one end of the extrusion rod (64) away from the sliding block (63) being fixedly connected to an extension elastic rod (65).

2. The embryonic stem cell bionic culture device according to claim 1, characterized in that: One end of the movable plate (13) away from the sliding plate (11) is fixedly connected to the surface of the sliding frame (45), the bottom of the right-angle plate (41) is fixedly connected to the surface of the workbench (1), one end of the expansion rod (52) away from the movable heating plate (51) is rotatably connected to the inner wall and top wall of the right-angle plate (41), and the number of the rising rods (44) is four, which are divided into two groups of two in each group, and the four rising rods (44) are symmetrically arranged with the culture dish (17) as the center.

3. The embryonic stem cell bionic culture device according to claim 1, characterized in that: The sealing mechanism (60) comprises a fixing frame (61), an end of the fixing frame (61) is fixedly connected to a slide plate (62), an end of the extending elastic rod (65) is fixedly connected to a fixing plate (66), both sides of the sliding block (63) are fixedly connected to pulling plates (67), an end of the pulling plate (67) away from the sliding block (63) is fixedly connected to a sealing box (68), a fixing groove is provided on a surface of the sealing box (68), and a merging plate (69) is fixedly connected to an end of the sealing box (68).

4. The embryonic stem cell bionic culture device according to claim 3, characterized in that: One end of the driving plate (47) away from the sliding frame (45) is fixedly connected to the inner wall of the sealing box (68), and a merging groove is provided at the end of the sealing box (68). One end of the extending elastic rod (65) away from the fixing plate (66) is fixedly connected to the inner wall of the slide plate (62), and one end of the fixing frame (61) away from the slide plate (62) is fixedly connected to the bottom of the workbench (1).