A tissue staining machine

Abstract

The present invention relates to a tissue staining machine, and belongs to the technical field of tissue staining machines. It includes a frame; a solution carrying structure, a placement plate is provided with a plurality of placement stations along its circumferential direction, and each placement station is used to accommodate different types of solution tanks; a driving structure, the driving structure includes a displacement component and a glass slide rack, and the displacement component is arranged on the frame; the glass slide rack has a variable volume chamber, and a plurality of air holes are opened on the circumferential wall surface of the glass slide rack, and the air holes are connected with the variable volume chamber; a pressing part is arranged on the displacement path of the glass slide rack. By arranging a variable volume chamber in the glass slide rack and utilizing a gas discharge mechanism, the drainage process is more active and powerful. Under the action of the pressing part, the chamber contracts and deforms, and the gas is forcefully discharged through the air holes, and the residual liquid on the surface of the sample is directly blown away. Compared with the rotation or dripping method, the gas discharge can more effectively remove the residual liquid adhering to the surface, and is particularly suitable for processing staining solutions with high viscosity.

Description

Technical Field

[0001] The invention belongs to the technical field of tissue dyeing machines, relates to a technology for improving the dyeing quality of a tissue dyeing machine, and specifically relates to a tissue dyeing machine. Background Art

[0002] In animal pathology research, pig tissue staining is a crucial experimental operation. By staining pig tissue samples, the cell structure and pathological changes inside the tissue can be revealed, providing an important basis for disease diagnosis, research and teaching. In actual operation, tissue staining usually requires soaking the tissue sample in multiple different staining and washing solutions in turn to obtain the best staining effect. However, existing tissue staining machines often face a technical problem when processing pig tissue samples, that is, the drainage is not thorough enough, which easily leads to mutual mixing between solutions, thus affecting the final staining quality.

[0003] Especially when staining pig tissue samples, due to the large structure and density of the tissue, the penetration and residue of different solutions during the staining process are more complicated. If the staining machine cannot effectively drain the previous solution, the residual solution may be mixed into the subsequent staining or washing solution, resulting in uneven staining or abnormal staining. This may not only affect the observation of the pathological characteristics of pig tissues under the microscope, but also mislead the subsequent experimental results and diagnostic judgments. Summary of the invention

[0004] In order to solve the above-mentioned problems in the prior art, the present invention provides a tissue staining machine.

[0005] In order to achieve the above object, the technical solution adopted by the present invention is:

[0006] Provided is a tissue staining machine, comprising:

[0007] frame;

[0008] A solution carrying structure, the solution carrying structure comprises a driving assembly and a placement plate, the driving assembly is arranged on the frame and connected to the placement plate, and is used to drive the placement plate to rotate, the placement plate is provided with a plurality of placement stations along its circumferential direction, and each placement station is used to accommodate different types of solution tanks;

[0009] A driving structure, the driving structure comprising a displacement assembly and a slide rack, the displacement assembly being arranged on the frame and located on one side of the placement tray, the displacement assembly being connected to the slide rack and being used to drive the slide rack to reciprocate in a direction perpendicular to the placement tray;

[0010] Wherein, the slide rack has a variable volume chamber, and a plurality of air holes are opened on the circumferential wall surface of the slide rack, and the air holes are connected with the variable volume chamber;

[0011] Wherein, a pressing portion is provided on the displacement path of the slide rack. When the slide rack moves to the position of the pressing portion, the pressing portion contacts the variable volume chamber, causing the variable volume chamber to shrink and deform, so that the gas is discharged to the surface of the slide rack through the air holes.

[0012] Preferably, the slide rack comprises:

[0013] Plastic part and elastic part;

[0014] Wherein, the plastic part is a hard material;

[0015] The elastic part is made of flexible material;

[0016] The plastic part and the elastic part enclose an annular variable volume chamber;

[0017] The circumferential wall surface of the plastic part is used to embed a plurality of glass slides and is provided with the air holes;

[0018] The elastic portion is used to abut against the pressing portion to compress the gas in the variable volume chamber.

[0019] Preferably, the plastic portion is formed with a heating cavity;

[0020] The variable volume chamber surrounds the outer circumference of the heating cavity;

[0021] Wherein, the heating cavity is filled with a heating medium.

[0022] Preferably, comprising a support arm;

[0023] The support arm is disposed on the frame and has a set length L2 in a direction perpendicular to the frame;

[0024] Wherein, the displacement assembly is mounted to the support arm and reciprocates along a set length L2;

[0025] Wherein, at least within the range of the set length L1, the pressing portion and the elastic portion abut against each other;

[0026] Moreover, L1=K*L2, and the value range of K is 0.2 to 0.6.

[0027] Preferably, a liquid collecting rack is included;

[0028] The liquid collecting rack is arranged at the end of the supporting arm;

[0029] Wherein, the pressing part is arranged in the liquid collecting frame;

[0030] An annular liquid collecting tank is provided at the bottom of the liquid collecting frame;

[0031] Furthermore, the liquid collecting rack is provided with an inner ring channel for the glass slide rack to pass through.

[0032] Preferably, comprising a drip-proof structure;

[0033] The anti-drip structure is arranged along the inner ring channel;

[0034] Wherein, the drip-proof structure is an annular structure, and the diameter of the inner ring thereof is smaller than the diameter of the slide rack;

[0035] Furthermore, the drip-proof structure is made of elastic material;

[0036] At least when the slide rack passes through the inner ring of the drip-proof structure, the diameter of the inner ring of the drip-proof structure increases, and after the slide rack passes through the inner ring of the drip-proof structure, the diameter of the inner ring of the drip-proof structure is restored.

[0037] Preferably, comprising an avoidance structure;

[0038] The avoidance structure is arranged on the glass slide rack and is located on both sides of each glass slide;

[0039] Wherein, the height of the avoidance structure protruding from the circumferential wall surface of the glass slide rack is greater than the height of the glass slide protruding from the circumferential wall surface of the glass slide rack.

[0040] Preferably, the liquid collecting tank comprises a flow guiding part and a collecting part;

[0041] Wherein, the guide part and the collection part are integrally formed;

[0042] Furthermore, the flow guide portion is an inclined annular surface;

[0043] And, the collecting portion is a horizontal annular surface.

[0044] Preferably, a sink body is included;

[0045] The confluence body is arranged on the bottom surface of the slide rack;

[0046] Wherein, the confluence body is a conical platform structure;

[0047] Furthermore, a plurality of confluence grooves are formed on the circumferential wall surface of the confluence body.

[0048] Preferably, comprising a negative pressure port;

[0049] The negative pressure port is opened on the bottom surface of the confluence body;

[0050] Wherein, the negative pressure port is connected to a negative pressure generating device.

[0051] The present invention provides a tissue dyeing machine, and the beneficial effects of the present invention are embodied in:

[0052] By setting up a variable volume chamber in the slide holder and using a gas exhaust mechanism, the drainage process is more active and powerful. The chamber contracts and deforms under the action of the pressing part, and the gas is forcefully discharged through the pores to directly blow away the residual liquid on the surface of the sample. Compared with rotation or dripping, gas exhaust can more effectively remove residual liquid adhering to the surface, especially suitable for processing high-viscosity staining solutions. BRIEF DESCRIPTION OF THE DRAWINGS

[0053] Figure 1 One of the three-dimensional diagrams of the tissue staining machine proposed by the present invention;

[0054] Figure 2 A front view of the tissue staining machine provided by the present invention;

[0055] Figure 3 The second stereogram of the tissue staining machine proposed by the present invention;

[0056] Figure 4 for Figure 3 A local enlarged schematic diagram of the structure shown at position A;

[0057] Figure 5 A three-dimensional diagram of a slide rack in a tissue staining machine provided by the present invention;

[0058] Figure 6 This is a front view of a slide rack in a tissue staining machine provided by the present invention;

[0059] Figure 7 A top view of a slide rack in a tissue staining machine provided by the present invention;

[0060] Figure 8 This is a cross-sectional view of a slide rack in the tissue staining machine proposed by the present invention.

[0061] Description of reference numerals:

[0062] 1. Rack; 2. Solution-carrying structure; 201. Drive assembly; 202. Placement plate; 2021. Placement station; 3. Drive structure; 301. Displacement assembly; 302. Slide rack; 3021. Variable volume chamber; 3022. Air hole; 3023. Plastic part; 3024. Elastic part; 3025. Heating chamber; 4. Pressing part; 5. Support arm; 6. Liquid collection rack; 601. Liquid collection tank; 6011. Guide part; 6012. Collecting part; 7. Drip-proof structure; 8. Avoidance structure; 9. Converging body. DETAILED DESCRIPTION

[0063] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0064] See also Figures 1 to 8 As shown, the specific embodiments provided by the present invention are as follows:

[0065] like Figures 1 to 3 As shown, the first embodiment of the present invention provides a tissue staining machine, comprising:

[0066] Rack 1;

[0067] A solution carrying structure 2, the solution carrying structure 2 comprises a driving assembly 201 and a placement plate 202, the driving assembly 201 is arranged on the frame 1 and connected to the placement plate 202, and is used to drive the placement plate 202 to rotate, the placement plate 202 is provided with a plurality of placement stations 2021 along its circumferential direction, and each placement station 2021 is used to accommodate different types of solution tanks;

[0068] A driving structure 3, the driving structure 3 comprising a displacement assembly 301 and a slide rack 302, the displacement assembly 301 being disposed on the frame 1 and located on one side of the placement tray 202, the displacement assembly 301 being connected to the slide rack 302 and being used to drive the slide rack 302 to reciprocate in a direction perpendicular to the placement tray 202;

[0069] The slide rack 302 has a variable volume chamber 3021, and a plurality of air holes 3022 are formed on the circumferential wall surface of the slide rack 302, and the air holes 3022 are connected to the variable volume chamber 3021;

[0070] A pressing portion 4 is provided on the displacement path of the glass slide rack 302. When the glass slide rack 302 moves to the position of the pressing portion 4, the pressing portion 4 contacts the variable volume chamber 3021, causing the variable volume chamber 3021 to shrink and deform, so that the gas is discharged to the surface of the glass slide rack 302 through the air holes 3022.

[0071] In this embodiment, the rack 1 serves as the skeleton structure of the tissue staining machine, providing overall support and stability. The design of the rack 1 not only needs to meet the installation requirements of the various components of the equipment, but also needs to ensure sufficient rigidity and durability during operation to cope with vibrations and load changes that may occur during the staining process. With the stable support of the rack 1, the entire staining process can be carried out without external interference, ensuring the accuracy and consistency of the staining.

[0072] In a specific embodiment, the rack 1 is a frame structure having a work surface for installing and carrying various structures.

[0073] The solution bearing structure 2 is one of the cores of this dyeing machine, mainly including a driving assembly 201 and a placement plate 202. The driving assembly 201 is mounted on the frame 1 and connected to the placement plate 202. Its function is to change the relative position of the sample and different solution tanks by rotating the placement plate 202. The design of the driving assembly 201 ensures the stability and accuracy of the rotation process to avoid unnecessary mechanical disturbances of the sample during the dyeing process. The placement plate 202 is provided with a plurality of placement stations 2021 along its circumferential direction, and each station is used to accommodate different types of solution tanks. Through the rotation of the driving assembly 201, the placement plate 202 can bring the sample into different solution tanks for dyeing or washing in turn. The material and surface treatment of the placement plate 202 should have good corrosion resistance to adapt to the various chemical solutions used in the dyeing process.

[0074] Compared with the traditional method, that is, the linear sample delivery method, the advantages of this structure are:

[0075] Traditional linear conveying methods usually require a long linear space to accommodate each solution tank. This layout takes up a large space, especially in limited spaces such as laboratories. Linear conveying may cause the overall size of the equipment to be larger, which is not conducive to layout and operation.

[0076] The rotating structure of the present invention: through the rotating design of the placement plate 202, multiple solution tanks can be arranged in a circumferential direction, which greatly reduces the footprint of the equipment. The placement plate 202 arranges different solution tanks compactly on a circumference, and realizes the conversion of samples between the solution tanks through rotation, thereby saving laboratory space and improving the integration of the equipment.

[0077] In the traditional linear transport method, the sample needs to be moved in sequence between the solution tanks in the linear transport system, and the overall path is long. Especially when processing multiple samples, the increase in the moving path may lead to a longer overall operation time and increase the risk of liquid remaining on the sample surface.

[0078] The rotating structure of the present invention: Since the solution tanks are arranged on the circumference, the moving path of the sample between the solution tanks is significantly shortened. During the rotation process, the distance of the sample from one station to another is shorter, the solution can be replaced more quickly, the time the sample is exposed to the air is reduced, and the risk of liquid evaporation or residue is reduced.

[0079] Traditional linear conveying method: Due to the long moving path, the sample needs more time to move between each station, which may make the overall staining process more time-consuming. At the same time, the movement of the sample between different solution tanks may cause inconsistent liquid residue due to the long path, affecting the staining effect.

[0080] The rotating structure of the present invention: The way the placement plate 202 rotates not only shortens the moving path of the sample, but also allows the sample to quickly and stably enter the next solution tank through precise drive control. This rotation method speeds up the dyeing process and significantly improves the overall operating efficiency. In addition, the rotation movement is smooth and accurate, which reduces the phenomenon of liquid dripping during sample movement and ensures the uniformity and consistency of the dyeing effect.

[0081] The traditional linear conveying method has low operational flexibility due to the fixed linear arrangement of the solution tanks. The number and types of solution tanks are usually limited by the conveying path and are difficult to adjust flexibly.

[0082] The rotating structure of the present invention: the placement plate 202 can be flexibly configured with different numbers and types of solution tanks, and can adapt to different experimental requirements by simply adjusting the position of the workstation. This design gives the dyeing machine higher operational flexibility, can better adapt to the needs under different experimental conditions, and provides more flexible solution configuration options.

[0083] In a specific embodiment, the driving assembly 201 is a motor, and the placement plate 202 is rotatably connected to the working surface of the frame 1 and is connected to the motor through a chain gear or a belt gear.

[0084] The driving structure 3 includes a displacement component 301 and a slide rack 302, which is responsible for delivering the sample slide into the solution tank in the form of lifting. The displacement component 301 is installed on one side of the frame 1 and is connected to the slide rack 302. The main function of this component is to drive the slide rack 302 to reciprocate in a direction perpendicular to the placement plate 202. The displacement component 301 should be designed with high precision to ensure that the slide rack 302 can accurately reach the predetermined position during each movement to avoid uneven staining caused by error accumulation.

[0085] The slide rack 302 is a structure that directly carries the sample and has a variable volume chamber 3021. The slide rack 302 needs to have sufficient strength and stability, and also needs to have a certain degree of flexibility to adapt to the deformation of the chamber and the gas discharge requirements. The design of the slide rack 302 directly affects the staining effect, so its material and structure must be strictly selected.

[0086] like Figures 7 and 8As shown, the variable volume chamber 3021 is a key component in the slide rack 302, which is responsible for discharging the gas in the chamber through deformation, thereby removing the residual liquid on the surface of the slide. A plurality of air holes 3022 are provided on the circumferential wall of the chamber, and the air holes 3022 are connected to the inside of the chamber. When the slide rack 302 moves to the position of the pressing part 4 during the displacement process, the pressing part 4 contacts the variable volume chamber 3021, causing the chamber to shrink and deform. The material of the chamber needs to have good elasticity and durability to ensure that it can still maintain good deformation ability and sealing performance after multiple uses.

[0087] The size and number of the air holes 3022 directly affect the gas exhaust efficiency. A reasonable design of the air holes 3022 can ensure that when the chamber contracts, sufficient gas is exhausted through the air holes 3022, thereby effectively blowing away the liquid residue on the surface of the glass slide. This design is particularly important when processing pig tissue samples, because the staining solution of pig tissue is often viscous and easy to remain, and it must be removed by a strong airflow to avoid cross-contamination of the staining solution.

[0088] The pressing part 4 is a fixed component arranged on the displacement path of the slide rack 302 , and is responsible for contacting with the variable volume chamber 3021 and causing it to shrink when the slide rack 302 moves to a specific position.

[0089] The design of the pressing part 4 needs to consider the degree of fit with the variable volume chamber 3021. The shape and material of the pressing part 4 should ensure that sufficient pressure can be accurately applied to cause the chamber to undergo the desired deformation without damaging the chamber. At the same time, the position setting of the pressing part 4 should be accurate to ensure that each time the slide rack 302 moves to this position, the contraction of the chamber can be accurately triggered, thereby achieving effective gas discharge.

[0090] Compared with the traditional drainage form, i.e., the drainage form of rotating drainage and waiting for automatic dripping, the advantages of this embodiment are:

[0091] Traditional spin leaching relies on centrifugal force to remove residual liquid from the sample surface. Although it can effectively remove some liquid, it is limited in effectiveness for more viscous staining solutions (such as pig tissue staining solutions). Centrifugal force acts unevenly on liquid at different locations, resulting in some liquid remaining on the sample surface, especially on more complex tissue surfaces.

[0092] Waiting for automatic dripping: This method completely relies on natural dripping due to gravity. The drainage process is slow and incomplete. Especially when dealing with more viscous solutions, the residual liquid can easily adhere to the sample surface, causing cross-contamination of different solutions.

[0093] In this embodiment, by setting a variable volume chamber 3021 in the slide rack 302 and utilizing a gas discharge mechanism, the drainage process is more active and effective. The chamber contracts and deforms under the action of the pressing portion 4, and the gas is forcefully discharged through the air hole 3022 to directly blow away the residual liquid on the surface of the sample. Compared with the rotation or dripping method, the gas discharge can more effectively remove the residual liquid adhering to the surface, and is particularly suitable for processing staining solutions with high viscosity.

[0094] Spin Drainage: During spin drainage, samples are subjected to centrifugal force, which may cause physical displacement or vibration of the samples. This method may cause the risk of uneven staining or damage to the samples, especially when dealing with more fragile or irregular tissue samples.

[0095] Waiting for automatic dripping: The process of waiting for dripping may cause inconsistency in dripping speed and effect due to slight vibrations or airflow in the external environment, thus affecting the subsequent dyeing effect.

[0096] In this embodiment, the vertical displacement of the slide rack 302 is combined with the design of gas exhaust to ensure the stability of the sample during the entire staining process. Whether it is gas blowing or mechanical movement, the sample can be kept in a predetermined position and state, avoiding uneven staining or damage caused by physical displacement, and ensuring high precision and stability of the staining process.

[0097] Rotary leaching: Rotary leaching usually takes a certain amount of time to achieve the desired effect, and because the rotation speed and time need to be controlled, the entire operation time is relatively long and the effect is limited.

[0098] Waiting for automatic dripping: The natural dripping method is a passive process. The drainage time is difficult to control and is easily affected by factors such as ambient temperature and liquid viscosity, resulting in long operation time and low efficiency.

[0099] This embodiment: Through mechanical pressing and gas discharge, the leaching process can be completed in a short time, significantly reducing the waiting time in the dyeing cycle. Due to the high efficiency of the leaching process, the present invention is significantly better than the rotation and dripping methods in terms of overall operation time, improving the working efficiency of the experiment.

[0100] In general, compared with the traditional rotary draining and waiting for automatic dripping draining methods, this embodiment has higher draining efficiency, better staining stability, shorter operation time and more consistent draining effect. These advantages enable the tissue staining machine of the present invention to provide higher quality and more reliable staining results when processing special samples such as pig tissues, significantly improving the overall efficiency and quality of the experiment.

[0101] In a specific implementation, the displacement assembly 301 is one of a pneumatic cylinder, a hydraulic cylinder, an electric push rod, or a linear slide rail.

[0102] like Figures 5 to 8 As shown, the second embodiment of the present invention provides a tissue staining machine, and based on the first embodiment, the slide rack 302 includes:

[0103] A plastic portion 3023 and an elastic portion 3024;

[0104] Wherein, the plastic part 3023 is a hard material;

[0105] The elastic portion 3024 is made of flexible material;

[0106] The plastic part 3023 and the elastic part 3024 enclose an annular variable volume chamber 3021;

[0107] The circumferential wall surface of the plastic part 3023 is used to embed a plurality of glass slides, and is provided with the air holes 3022;

[0108] The elastic portion 3024 is used to abut against the pressing portion 4 to compress the gas in the variable volume chamber 3021 .

[0109] In this embodiment, the slide rack 302 is designed as a cylindrical structure, which further optimizes the effect of gas exhaust and liquid removal. Specifically, the slide rack 302 is composed of a plastic part 3023 and an elastic part 3024, which respectively occupy different circumferential walls of the cylindrical structure, thereby achieving the function of taking both rigidity and elasticity into account.

[0110] In the conventional design of the slide rack 302, the irregular shape or complex structure may lead to uneven gas discharge, thereby affecting the liquid removal efficiency during the staining process. Especially in high-frequency operations, the irregular structure may also increase the difficulty of operation and mechanical wear.

[0111] In this embodiment, a cylindrical structure is adopted, so that the slide rack 302 has good symmetry and structural stability during the staining process. The cylindrical design not only simplifies the manufacturing process, but also enhances the durability of the overall structure and reduces the wear problem caused by mechanical friction. In addition, the cylindrical structure makes the deformation of the variable volume chamber 3021 more uniform, which helps to achieve uniform gas discharge.

[0112] The application of the cylindrical structure ensures the symmetry and uniformity of the gas during the discharge process, avoiding the problem of local poor discharge or insufficient gas pressure. This design enables the dyeing machine to more effectively remove residual liquid on the surface when processing different types of tissue samples, especially pig tissue samples, to ensure the stability and consistency of the dyeing quality.

[0113] The plastic part 3023 occupies a part of the circumferential wall of the cylindrical structure, forming a solid shell of the slide rack 302. Since the plastic part 3023 is made of hard material, its main function is to provide necessary structural support and stability, ensuring that when the variable volume chamber 3021 is deformed, the position and size of the air hole 3022 remain unchanged, thereby ensuring the stability of the gas discharge path.

[0114] By arranging the plastic part 3023 on the circumferential wall of the cylinder, the problem of blockage or size change of the pores 3022 due to structural deformation during the deformation process can be effectively avoided. This design ensures that the gas can be discharged smoothly through the pores 3022, thereby improving the removal efficiency of residual liquid during the dyeing process.

[0115] The elastic part 3024 is located on another circumferential wall of the cylindrical structure and is made of a flexible material. The main function of this part is to deform by contacting the pressing part 4, compress the gas in the variable volume chamber 3021, and eject it through the air hole 3022 on the plastic part 3023.

[0116] The flexible design of the elastic part 3024 enables it to deform when subjected to external force (such as the pressure of the pressing part 4), thereby effectively compressing the gas in the chamber. During the deformation process, the elastic part 3024 can evenly transmit pressure, ensuring that the process of gas ejecting through the air hole 3022 is smooth and consistent, avoiding intermittent or unstable conditions in the gas flow.

[0117] In traditional designs, gas exhaust is often affected by uneven deformation or insufficient pressure, resulting in unstable airflow during exhaust, which in turn affects the quality of the dyeing process.

[0118] By designing the elastic part 3024 as a part of the circumferential wall of the cylindrical structure, when squeezing is applied to it, the gas in the entire variable volume chamber 3021 can be uniformly compressed. Under the action of uniform pressure, the gas is ejected through the air holes 3022 on the plastic part 3023 to form a stable and strong airflow. This design effectively solves the problem of airflow fluctuation caused by uneven pressure and ensures that the residual liquid on the surface of the slide can be completely removed during the staining process.

[0119] Compared with the conventional flat or irregularly shaped slide rack 302, the cylindrical structure exhibits higher stability and efficiency in the deformation and gas discharge process. By squeezing the elastic portion 3024, the gas in the variable volume chamber 3021 can be uniformly and stably ejected, further improving the accuracy and uniformity of the staining process.

[0120] The tissue staining machine in this embodiment achieves an efficient and stable gas discharge process by adopting a cylindrical slide rack 302 design and cleverly combining the advantages of the plastic part 3023 and the elastic part 3024. The plastic part 3023 provides the necessary structural support and stability of the pores 3022, while the elastic part 3024 achieves effective compression and ejection of the gas through deformation. This design significantly improves the drainage effect, especially when processing pig tissue samples, it can more thoroughly remove residual liquid on the surface, ensure that the solution is replaced more thoroughly during the staining process, and thus improve the uniformity and accuracy of the staining. Compared with traditional designs, this embodiment has significant technical advantages in structural stability, ease of operation and gas discharge efficiency.

[0121] like Figure 8 As shown, the third embodiment of the present invention provides a tissue dyeing machine, and based on the previous embodiment, the plastic part 3023 is formed with a heating cavity 3025;

[0122] The variable volume chamber 3021 surrounds the outer circumference of the heating chamber 3025;

[0123] The heating cavity 3025 is filled with a heating medium.

[0124] In this embodiment, the tissue staining machine further optimizes the structure of the slide rack 302 and adds a heating function to achieve more efficient blowing and drying effects. This improvement further improves the overall efficiency of the staining process and the processing quality of the sample while maintaining the gas exhaust effect. The following is a detailed analysis of the key technical features of this embodiment:

[0125] During tissue staining, liquid residue on the sample surface must not only be removed, but also dried as quickly as possible to avoid reattachment or incomplete evaporation of the liquid, which may affect the staining effect. Although traditional gas exhaust methods can remove liquid, for samples that are difficult to dry (such as pig tissue), simple airflow may not achieve the ideal drying effect.

[0126] In this embodiment, a heating cavity 3025 is formed in the plastic part 3023, and the variable volume chamber 3021 is surrounded on the outer circumference of the heating cavity 3025. The heating cavity 3025 is filled with a heating medium, which can heat the gas in the variable volume chamber 3021. When the gas is ejected through the air hole 3022, the heated gas can more effectively dry the residual liquid on the surface of the sample, significantly improving the drying speed and effect.

[0127] The introduction of the heating chamber 3025 not only improves the drying ability of the gas, but also enhances the removal effect of the liquid on the sample surface, especially when dealing with more viscous liquids. This heating function can prevent the liquid from re-condensing or remaining after being blown away, thereby improving the uniformity of staining and the processing quality of the sample.

[0128] The conventional slide rack 302 usually adopts a single-layer design. Although this design is simple in structure, it is often limited when adding functions. In particular, when it is necessary to realize gas exhaust and heating functions at the same time, the single-layer structure is difficult to take into account both.

[0129] This embodiment designs a double-layer slide rack 302 structure, that is, the slide rack 302 is divided into an inner layer and an outer layer. The outer layer is composed of a plastic part 3023 and an elastic part 3024, which is responsible for carrying the sample and realizing the compression and discharge functions of the gas; the inner layer is composed of the plastic part 3023 and forms a heating cavity 3025. A variable volume chamber 3021 is arranged between the inner and outer layers, so that the volume of the variable volume chamber 3021 is relatively small, ensuring that during the gas compression process, the gas can be ejected at a higher speed to improve the blowing effect.

[0130] The double-layer structure design effectively combines the heating function and the gas exhaust function, which not only ensures the speed and efficiency of gas outflow, but also reduces the volume and weight of the entire slide rack 302 through a compact structure design, thereby improving the operational flexibility and the overall performance of the equipment.

[0131] In traditional staining equipment, the drying process often requires additional equipment or steps, which increases the complexity and time cost of the operation. Especially when processing pig tissue samples, thicker tissue samples take longer to completely dry, which may affect the staining effect.

[0132] By heating the gas in the heating chamber 3025 and blowing it toward the sample surface, this embodiment can achieve rapid drying of the sample while blowing away the liquid. The heated gas not only helps to more thoroughly remove the residual liquid, but also accelerates the evaporation of the liquid, ensuring that the sample surface becomes dry in a short time, thereby shortening the staining cycle and improving the experimental efficiency.

[0133] The heated gas drying function significantly simplifies the staining process, avoids additional drying steps, and improves the efficiency of the entire operation process. For difficult samples such as porcine tissue samples, heated drying can ensure that the samples are in the best condition before staining, improving the quality and reliability of the final staining.

[0134] In this embodiment, the tissue staining machine realizes the blowing and drying functions of the heated gas by introducing a heating chamber 3025 into the slide rack 302. The double-layer slide rack 302 design not only effectively combines the gas exhaust and heating functions, but also optimizes the structural volume and gas flow rate, ensuring the liquid removal and drying efficiency during the staining process. Compared with the traditional single-layer slide rack 302 and the staining equipment without heating function, this embodiment provides a more efficient, flexible and comprehensive solution, which is particularly suitable for the processing of complex samples with high requirements for staining quality, such as the staining of pig tissue. Through this innovative design, the present invention not only improves the accuracy and consistency of the staining process, but also greatly shortens the operation time, laying a solid foundation for high-quality microscopic analysis.

[0135] The fourth embodiment of the present invention provides a tissue staining machine, and based on the previous embodiment, includes a support arm 5;

[0136] The support arm 5 is disposed on the frame 1 and has a set length L2 in a direction perpendicular to the frame 1;

[0137] Wherein, the displacement assembly 301 is mounted to the support arm 5 and reciprocates along a set length L2;

[0138] Wherein, at least within the range of the set length L1, the pressing portion 4 and the elastic portion 3024 abut against each other;

[0139] Moreover, L1=K*L2, and the value range of K is 0.2 to 0.6.

[0140] In this embodiment, the design of the tissue staining machine is further optimized on the basis of the above, and the operating flexibility and accuracy of the equipment are enhanced by introducing the support arm 5 structure. The following is a detailed analysis of the key technical features of this embodiment:

[0141] In this embodiment, by arranging a support arm 5 on the frame 1, the displacement assembly 301 can reciprocate along a set length L2 in a direction perpendicular to the frame 1 (the displacement assembly 301 is in the form of a linear slide rail). The introduction of the support arm 5 provides a stable motion trajectory and support for the displacement assembly 301, ensures the stability and accuracy of the slide rack 302 during the movement process, and reduces the impact of mechanical errors on the staining quality.

[0142] During the staining process, the movement path and distance of the sample between the solution tanks need to be highly accurate to avoid uneven staining or solution cross-contamination. In traditional designs, the displacement component 301 is often limited by the structure, making it difficult to accurately control the movement distance and speed, especially in complex staining procedures, where errors may accumulate.

[0143] In the previous embodiment, the discharge of gas in the variable volume chamber 3021 is triggered by the contact between the pressing portion 4 and the elastic portion 3024, thereby removing the residual liquid on the sample surface. However, in actual operation, the contact position and time between the pressing portion 4 and the elastic portion 3024 need to be precisely controlled, otherwise it may cause uneven or delayed gas discharge, affecting the final staining quality.

[0144] In this embodiment, the pressing portion 4 and the elastic portion 3024 are in contact with each other within the range of setting the length L1, thereby ensuring that the pressing portion 4 can contact the elastic portion 3024 at the optimal position and time during the entire movement process. The length L1 is set to be 0.2 to 0.6 times the total length L2, that is, L1=K*L2, and the value range of K is 0.2 to 0.6. This range setting enables the pressing portion 4 and the elastic portion 3024 to interact with each other within the most effective range, ensuring that the gas in the variable volume chamber 3021 is discharged at the optimal time.

[0145] This embodiment ensures high efficiency and consistency of the gas discharge process by precisely controlling the contact time and position of the pressing portion 4 and the elastic portion 3024. Compared with the traditional passive or imprecise design, this active control design greatly improves the efficiency of the drainage and avoids liquid residue or contamination during the dyeing process.

[0146] like Figure 3 to Figure 4 As shown, the fifth embodiment of the present invention provides a tissue staining machine, and based on the previous embodiment, includes a liquid collection rack 6;

[0147] The liquid collecting rack 6 is arranged at the end of the supporting arm 5;

[0148] Wherein, the pressing portion 4 is disposed in the liquid collecting frame 6;

[0149] An annular liquid collecting tank 601 is provided at the bottom of the liquid collecting frame 6;

[0150] Furthermore, the liquid collecting rack 6 is provided with an inner ring channel for the glass slide rack 302 to pass through.

[0151] In this embodiment, the tissue staining machine is further optimized on the basis of the above-mentioned design, and the liquid collection and processing capabilities are enhanced by introducing the liquid collection rack 6 structure, thereby improving the cleanliness and operating efficiency of the staining process. The following is a detailed analysis of the key technical features of this embodiment:

[0152] During the staining process, especially when the liquid is replaced and handled multiple times, the residual staining liquid is easy to drip and contaminate the equipment or sample surface, thus affecting the staining quality. In traditional designs, there is often a lack of effective liquid collection mechanism, which leads to improper liquid handling and causes a series of problems.

[0153] This embodiment effectively solves the problem of liquid dripping and contamination by arranging a liquid collecting rack 6 at the end of the support arm 5. The design of the liquid collecting rack 6 allows all liquids discharged through the glass slide rack 302 to be collected centrally, preventing the liquid from being scattered inside the device or on the surface of the sample. The combination of the liquid collecting rack 6 and the support arm 5 enables precise positioning, ensuring that the liquid directly enters the liquid collecting tank 601 after being discharged.

[0154] The introduction of the liquid collecting rack 6 not only improves the efficiency of liquid processing, but also greatly reduces the risk of contamination of equipment and samples by liquid dripping. This design is particularly suitable for processing large-volume or high-viscosity staining liquids, ensuring the cleanliness and safety of the staining environment.

[0155] In the previous embodiment, the contact between the pressing portion 4 and the elastic portion 3024 triggers the gas discharge, thereby removing the residual liquid on the sample surface. However, the gas discharge is often accompanied by the discharge of liquid, which may scatter and cause pollution of the staining environment if not controlled.

[0156] In this embodiment, the pressing part 4 is arranged in the liquid collecting frame 6, so that when the pressing part 4 triggers the gas discharge, the discharged liquid can be directly collected by the liquid collecting frame 6. This design not only simplifies the liquid collection process, but also ensures that the surrounding environment will not be polluted when the liquid is discharged. The combination design of the pressing part 4 and the liquid collecting frame 6 makes the liquid treatment more efficient and clean.

[0157] Compared with the traditional liquid handling method, this embodiment ensures the simultaneous discharge of gas and collection of liquid through the synergistic effect of the liquid collecting rack 6 and the pressing part 4, avoiding the risk of liquid scattering and contamination. Especially when dealing with complex dyeing procedures, this design can significantly improve the overall cleanliness and operating efficiency of dyeing.

[0158] During the staining process, the handling of residual liquid is often a problem. In traditional designs, the liquid may be scattered irregularly, making it difficult to centrally handle or clean it, which in turn affects the operating efficiency of the equipment and the staining effect of the sample.

[0159] In this embodiment, an annular liquid collecting trough 601 is arranged at the bottom of the liquid collecting frame 6. The annular design ensures that the liquid can be evenly distributed and concentrated in the liquid collecting trough 601. No matter from which direction the liquid is discharged, it can be smoothly guided to the liquid collecting trough 601, avoiding liquid overflow or scattering.

[0160] The design of the annular liquid collecting trough 601 greatly improves the efficiency and cleanliness of liquid collection, especially when dealing with dyeing procedures that are repeated multiple times. This design can ensure the efficiency and consistency of liquid processing and reduce the risk of contamination caused by liquid spillage.

[0161] Compared with the traditional liquid handling method, this embodiment shows significant advantages in liquid collection, pollution control and equipment maintenance. Especially under experimental conditions that deal with complex staining procedures or require high-precision operations, the design of the liquid collection rack 6 ensures that the liquid handling of each staining step can be completed efficiently and cleanly, thereby improving the overall quality and reliability of the experiment.

[0162] like Figure 4 As shown, the sixth embodiment of the present invention provides a tissue staining machine, and based on the previous embodiment, includes a drip-proof structure 7;

[0163] The anti-drip structure 7 is arranged along the inner ring channel;

[0164] The drip-proof structure 7 is an annular structure, and the diameter of the inner ring thereof is smaller than the diameter of the glass slide rack 302;

[0165] Furthermore, the drip-proof structure 7 is made of elastic material;

[0166] At least when the slide rack 302 passes through the inner ring of the drip-proof structure 7 , the diameter of the inner ring of the drip-proof structure 7 increases, and after the slide rack 302 passes through the inner ring of the drip-proof structure 7 , the diameter of the inner ring of the drip-proof structure 7 recovers.

[0167] In this embodiment, the main purpose of the avoidance structure 8 is to optimize the interaction between the slide rack 302 and the drip-proof structure 7, so that the slide rack 302 can smoothly enter the liquid collection rack 6, while preventing the drip-proof structure 7 from directly contacting the slide, thereby ensuring the smooth progress of the staining process and the integrity of the sample. The following is a detailed analysis of the key technical features of this embodiment:

[0168] During the staining process, the inner ring of the drip-proof structure 7 is designed to be slightly smaller than the diameter of the slide rack 302 to provide the necessary sealing effect to prevent liquid from dripping. However, when the slide rack 302 passes through the drip-proof structure 7, if there is no appropriate guiding or opening mechanism, the inner ring of the drip-proof structure 7 may directly contact the slide, causing damage to the sample or obstruction of movement, affecting the smooth progress of the staining operation.

[0169] In this embodiment, the avoidance structures 8 are arranged on both sides of the glass slide, so that when the glass slide rack 302 enters the drip-proof structure 7, the avoidance structures 8 can first contact and open the inner ring of the drip-proof structure 7. This process ensures that the glass slide rack 302 can pass through the drip-proof structure 7 smoothly, and the inner ring of the drip-proof structure 7 will not directly contact the glass slide when the avoidance structures 8 are opened, thereby avoiding damage to the sample.

[0170] The introduction of the avoidance structure 8 significantly improves the coordination between the slide rack 302 and the drip-proof structure 7, avoiding physical friction or jamming that may occur during operation. This design ensures the smooth movement of the slide rack 302, improves the efficiency of the overall operation, and protects the integrity of the sample, which is particularly critical when handling more fragile or important tissue samples.

[0171] Furthermore, when the slide rack 302 enters the liquid collecting rack 6, if the anti-drip structure 7 fails to effectively open or avoid, the slide rack 302 may encounter obstacles, resulting in an unsmooth liquid handling process, and may even affect the efficiency of liquid collection and the staining effect. If it directly contacts the slide, it may cause physical pressure on the sample, causing the slide to break or the sample to shift, affecting the accuracy and effect of staining.

[0172] Through the design of the avoidance structure 8, when the slide rack 302 approaches the drip-proof structure 7, the avoidance structure 8 first opens the inner ring of the drip-proof structure 7 to ensure that the slide rack 302 can smoothly enter the liquid collection rack 6. This design avoids the slide rack 302 from being blocked when passing through the inner ring channel, making the entire liquid processing process smoother and more efficient.

[0173] The function of the avoidance structure 8 is not only to protect the slides, but also to ensure that the slide rack 302 can smoothly enter and exit the liquid collection rack 6 during the entire operation, thereby reducing friction and errors in the operation. In particular, the guiding and opening functions of the avoidance structure 8 help maintain the continuity and consistency of the operation during repeated operations.

[0174] Moreover, the avoidance structure 8 effectively prevents the potential damage to the glass slide by the drip-proof structure 7, making the staining process safer and more reliable. This design is particularly suitable for use under operating conditions that require high precision and high protection, ensuring the integrity of the sample throughout the staining process and the consistency of the staining results.

[0175] In a specific embodiment, the avoidance structure 8 is a plate-like structure arranged along the length direction of the circumferential wall of the slide rack 302 .

[0176] The eighth embodiment of the present invention provides a tissue staining machine, and based on the previous embodiment, the liquid collecting tank 601 includes a guide part 6011 and a collection part 6012;

[0177] Wherein, the guide part 6011 and the collection part 6012 are integrally formed;

[0178] Furthermore, the flow guide portion 6011 is an inclined annular surface;

[0179] Furthermore, the collecting portion 6012 is a horizontal annular surface.

[0180] In this embodiment, the tissue staining machine, based on the above-mentioned design, further optimizes the structural design of the liquid collecting tank 601 and introduces an integrated structure of the flow guide part 6011 and the collection part 6012. This improvement is intended to improve the collection efficiency and treatment effect of the liquid and ensure the cleanliness and operation efficiency of the staining process. The following is a detailed analysis of the key technical features of this embodiment:

[0181] During the liquid collection process, the liquid may spread or accumulate on the plane, making it difficult for some of the liquid to effectively flow into the collection tank, thereby affecting the efficiency of liquid processing and the cleanliness of the equipment.

[0182] In this embodiment, the guide portion 6011 of the liquid collecting tank 601 is designed as an inclined annular surface. The inclined design allows the liquid to be quickly guided to the collection portion 6012 after being discharged, thereby preventing the liquid from being retained or diffused in the guide portion 6011. Through this inclined structure, the liquid can flow quickly by gravity, thereby reducing the liquid from staying in unnecessary areas.

[0183] The inclined annular surface design of the guide part 6011 significantly improves the speed and directionality of the liquid flow, ensuring that the liquid can efficiently enter the collection part 6012, reducing the residence time of the liquid in the equipment, reducing the difficulty of cleaning, and preventing potential contamination problems caused by liquid accumulation.

[0184] During the liquid discharge process, once the liquid enters the collection tank, there needs to be enough space and appropriate structure to accommodate and handle the liquid. If the collection part 6012 is not designed properly, it may cause liquid overflow or be difficult to centrally handle, affecting the normal operation of the equipment.

[0185] In this embodiment, the collecting portion 6012 of the liquid collecting tank 601 is designed as a horizontal annular surface to ensure that the liquid can be smoothly collected in the collecting portion 6012 for centralized treatment after being guided by the flow guide portion 6011. The horizontal design provides sufficient volume and a stable liquid storage environment, avoiding liquid overflow or uneven treatment.

[0186] like Figure 6 As shown, the ninth embodiment of the present invention provides a tissue staining machine, and based on the previous embodiment, includes a sink body 9;

[0187] The confluence body 9 is disposed on the bottom surface of the slide rack 302;

[0188] Wherein, the confluence body 9 is a conical platform structure;

[0189] Furthermore, a plurality of confluence grooves are formed on the circumferential wall surface of the confluence body 9 .

[0190] In this embodiment, the main function of the conduit 9 is to accelerate the dripping of the residual solution at the bottom of the slide rack 302, thereby improving the efficiency of liquid discharge and the cleanliness of the staining process. The following is a detailed analysis of the key technical features of this embodiment:

[0191] During the staining process, the residual solution at the bottom of the slide rack 302 is often difficult to remove quickly, especially when the liquid viscosity is high or the liquid volume is small, the liquid is easy to stay at the bottom of the slide rack 302. This residue not only increases the difficulty of cleaning, but also may cause cross contamination between samples, affecting the staining quality.

[0192] In this embodiment, a concentrator 9 is provided on the bottom surface of the slide rack 302 to accelerate the dripping of the residual solution at the bottom. The concentrator 9 is designed as a conical platform structure, and uses gravity to guide the liquid to quickly gather at the bottom of the cone. This structural design ensures that the liquid will not be retained at the bottom of the slide rack 302, but will quickly gather and drip, thereby accelerating the discharge process of the liquid.

[0193] The design of the confluence body 9 effectively solves the problem of liquid retention and significantly improves the efficiency of the dyeing operation and the cleanliness of the equipment by accelerating the dripping process of the liquid. Compared with the traditional flat or irregular design, the combination of the conical platform structure and the confluence groove makes the liquid discharge more rapid and orderly, avoiding the influence of residual liquid on subsequent operations.

[0194] The conical platform structure greatly improves the efficiency of liquid collection and dripping, ensuring that the liquid can be discharged in time during the dyeing process, reducing the amount of residual liquid. This design is particularly suitable for use in dyeing operations that require fast cleaning and efficient liquid management, further improving the operating efficiency and dyeing quality of the equipment.

[0195] In one embodiment, a negative pressure port is included;

[0196] The negative pressure port is opened on the bottom surface of the confluence body 9;

[0197] Wherein, the negative pressure port is connected to a negative pressure generating device.

[0198] The tissue staining machine further optimizes the liquid discharge and collection system, and further improves the liquid discharge efficiency by opening a negative pressure port on the bottom surface of the confluence body 9 and connecting it to the negative pressure generating device. The following is a detailed analysis of this embodiment:

[0199] During the liquid discharge process, especially for liquids with higher viscosity, gravity discharge may sometimes be insufficient to quickly remove the liquid, resulting in liquid retention at the bottom of the slide rack 302 or in the sink 9. This retention not only reduces the operating efficiency, but also may cause cross contamination between samples, affecting the staining quality.

[0200] By providing a negative pressure port on the bottom surface of the confluence body 9 and connecting it to a negative pressure generating device, the discharge process of the liquid can be effectively accelerated by utilizing the effect of negative pressure. The negative pressure port can generate suction to quickly draw out the liquid in the confluence body 9, reduce the retention time of the liquid at the bottom, and ensure that the liquid can be discharged and collected in time.

[0201] The introduction of the negative pressure port significantly improves the speed and efficiency of liquid discharge, especially when dealing with liquids with high viscosity or difficult to discharge, the negative pressure effect can effectively prevent liquid retention. This design not only improves the efficiency of dyeing operations, but also reduces the residual liquid inside the equipment, keeping the operating environment clean.

[0202] In the description of the embodiments of the present invention, it needs to be understood that terms such as “up”, “down”, “front”, “back”, “left”, “right”, “vertical”, “horizontal”, “center”, “top”, “bottom”, “top”, “bottom”, “inside”, “outside”, “inside”, and “outside” indicate orientation or positional relationships.

Claims

1. A tissue dyeing machine, characterized in that: include: frame; A solution carrying structure, the solution carrying structure comprises a driving assembly and a placement plate, the driving assembly is arranged on the frame and connected to the placement plate, and is used to drive the placement plate to rotate, the placement plate is provided with a plurality of placement stations along its circumferential direction, and each placement station is used to accommodate different types of solution tanks; A driving structure, the driving structure comprising a displacement assembly and a slide rack, the displacement assembly being arranged on the frame and located on one side of the placement tray, the displacement assembly being connected to the slide rack and being used to drive the slide rack to reciprocate in a direction perpendicular to the placement tray; Wherein, the slide rack has a variable volume chamber, and a plurality of air holes are opened on the circumferential wall surface of the slide rack, and the air holes are connected with the variable volume chamber; Wherein, a pressing portion is provided on the displacement path of the slide rack. When the slide rack moves to the position of the pressing portion, the pressing portion contacts the variable volume chamber, causing the variable volume chamber to shrink and deform, so that the gas is discharged to the surface of the slide rack through the air holes.

2. The tissue dyeing machine according to claim 1, characterized in that: The slide rack comprises: Plastic part and elastic part; Wherein, the plastic part is a hard material; The elastic part is made of flexible material; The plastic part and the elastic part enclose an annular variable volume chamber; The circumferential wall surface of the plastic part is used to embed a plurality of glass slides and is provided with the air holes; The elastic portion is used to abut against the pressing portion to compress the gas in the variable volume chamber.

3. The tissue dyeing machine according to claim 2, characterized in that: The plastic part is formed with a heating cavity; The variable volume chamber surrounds the outer circumference of the heating cavity; Wherein, the heating cavity is filled with a heating medium.

4. The tissue dyeing machine according to claim 3, characterized in that: Includes support arm; The support arm is disposed on the frame and has a set length L2 in a direction perpendicular to the frame; Wherein, the displacement assembly is mounted to the support arm and reciprocates along a set length L2; Wherein, at least within the range of the set length L1, the pressing portion and the elastic portion abut against each other; Moreover, L1=K*L2, and the value range of K is 0.2 to 0.

6.

5. The tissue dyeing machine according to claim 4, characterized in that: Includes liquid collection rack; The liquid collecting rack is arranged at the end of the supporting arm; Wherein, the pressing part is arranged in the liquid collecting frame; An annular liquid collecting tank is provided at the bottom of the liquid collecting frame; Furthermore, the liquid collecting rack is provided with an inner ring channel for the glass slide rack to pass through.

6. The tissue dyeing machine according to claim 5, characterized in that: Includes drip-proof construction; The anti-drip structure is arranged along the inner ring channel; Wherein, the drip-proof structure is an annular structure, and the diameter of the inner ring thereof is smaller than the diameter of the slide rack; Furthermore, the drip-proof structure is made of elastic material; At least when the slide rack passes through the inner ring of the drip-proof structure, the diameter of the inner ring of the drip-proof structure increases, and after the slide rack passes through the inner ring of the drip-proof structure, the diameter of the inner ring of the drip-proof structure is restored.

7. The tissue dyeing machine according to claim 6, characterized in that: including avoidance structures; The avoidance structure is arranged on the glass slide rack and is located on both sides of each glass slide; Wherein, the height of the avoidance structure protruding from the circumferential wall surface of the glass slide rack is greater than the height of the glass slide protruding from the circumferential wall surface of the glass slide rack.

8. The tissue dyeing machine according to claim 7, characterized in that: The liquid collecting tank comprises a flow guiding part and a collecting part; Wherein, the guide part and the collection part are integrally formed; Furthermore, the flow guide portion is an inclined annular surface; And, the collecting portion is a horizontal annular surface.

9. The tissue dyeing machine according to claim 2, characterized in that: Includes sink body; The confluence body is arranged on the bottom surface of the slide rack; Wherein, the confluence body is a conical platform structure; Furthermore, a plurality of confluence grooves are formed on the circumferential wall surface of the confluence body.

10. The tissue dyeing machine according to claim 9, characterized in that: Including negative pressure port; The negative pressure port is opened on the bottom surface of the confluence body; Wherein, the negative pressure port is connected to a negative pressure generating device.

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