Breeding method of sisal hemp and neo-pineapple mealybugs

Abstract

The invention discloses a sisal hemp neo-pineapple mealybug breeding method which comprises the following steps: (1) selecting hydroponic pineapples as host plants, putting the hydroponic pineapples into a culture room, and transferring female adult sisal hemp neo-pineapple mealybug to be born onto the hydroponic pineapples; (2) after the female adult sisal hemp new pineapple mealybug grey insects produce nymphs, new hydroponic pineapples are placed every 10-15 days, the new hydroponic pineapples are placed at the positions partially overlapped with the leaves of the raw hydroponic pineapples, the raw hydroponic pineapples are taken out after the new hydroponic pineapples are placed for 1-2 days, the step of replacing the hydroponic pineapples is repeated, and the new hydroponic pineapples are taken out after the new hydroponic pineapples are placed in the positions partially overlapped with the leaves of the raw hydroponic pineapples. And carrying out subculture feeding on the sisal hemp and the neo-pineapple mealybug. By means of the method, the sisal hemp neo-pineapple mealybug can be bred in a laboratory, and the method has important significance on prevention and control research on the sisal hemp neo-pineapple mealybug.

Description

Technical Field

[0001] The invention relates to the technical field of agricultural insect breeding, in particular to a method for breeding sisal mealybugs. Background Art

[0002] The sisal mealybug is an important invasive pest of the genus Mealycosidae in the superfamily Coccideoidea of ​​the order Hemiptera. It first broke out in Qingkan Farm, Changjiang City, Hainan Province, my country in 1998, and spread to the sisal in Changjiang and adjacent areas in 2001, harming 100% of the plants. The insect population density is high, with up to tens of thousands of mealybugs damaging a single sisal plant. The purple leaf roll disease caused by the insect has also broken out, resulting in an annual production reduction of more than 30%, or even a complete loss of harvest, which seriously threatens the development of the sisal industry.

[0003] In addition to harming sisal, the sisal pineapple mealybug also harms 76 host plants in 39 families such as pineapple and citrus, seriously affecting the yield and quality of plants and affecting the development of agriculture.

[0004] The damage caused by the gray mealybug on sisal pineapple is quite serious. Therefore, raising the gray mealybug on sisal pineapple under laboratory conditions so as to conduct more in-depth biological research and comprehensive prevention and control technology research will be of great significance to the prevention and control of the gray mealybug on sisal pineapple. Summary of the invention

[0005] The invention provides a method for raising the sisal hemp mealybug, which can realize the raising of the sisal hemp mealybug in a laboratory and has important significance for the research on the prevention and treatment of the sisal hemp mealybug.

[0006] To achieve the above object, the technical solution of the present invention is:

[0007] A method for raising mealybugs of sisal hemp comprises the following steps:

[0008] (1) selecting a hydroponic pineapple as a host plant and placing it in a culture room, transferring the female adults of the sisal new pineapple mealybug to be laid to the hydroponic pineapple;

[0009] (2) after the female adult of the sisal new pineapple mealybug produces nymphs, a new hydroponic pineapple is placed every 10-15 days, and the new hydroponic pineapple is placed in a position where the leaves of the new hydroponic pineapple partially overlap with those of the original hydroponic pineapple. After the new hydroponic pineapple is placed for 1-2 days, the original hydroponic pineapple is taken out, and the above-mentioned step of replacing the hydroponic pineapple is repeated to carry out successive generation rearing of the sisal new pineapple mealybug;

[0010] In steps (1) and (2), the rearing conditions in the culture room are: temperature 22-26°C, humidity 80-90%, and photoperiod of (11-13)L:(13-11)D.

[0011] Further, in step (2), when the sisal new pineapple gray mealybug nymphs grow to the second instar, ants are introduced, and the number of ants introduced per hydroponic pineapple is 10-20. Further, in step (2), after the ants are introduced, when the new hydroponic pineapple is placed, pineapple juice is applied to the roots of the pineapple leaves.

[0012] Furthermore, in step (1), 6-10 female adults of the sisal mealybug are transferred to each hydroponic pineapple plant.

[0013] Furthermore, in step (2), when a new hydroponic pineapple is placed, a rubber stopper soaked in mealybug attractant is hung on the top of the hydroponic pineapple.

[0014] Furthermore, the hydroponic pineapple is pre-cultivated before being placed in the cultivation room, and the cultivation steps include:

[0015] S1. Take pineapple plants with strong terminal buds as seedlings, cut the terminal buds and keep the base of the terminal buds intact, and dry them in the sun until the cut is dry;

[0016] S2. the pineapple terminal buds dried in the sun are placed in a sterilized container for hydroponics, the pineapple terminal buds are immersed in water for 0.5-1cm to wait for them to take root, and the water is changed every 2-3 days during the rooting period; when the roots grow to more than 3cm, a nutrient solution is placed in a container to continue hydroponics, and the nutrient solution is composed of the following ingredients: 1 / 32-1 / 64MS nutrient solution, 0.1-0.3mg / L boric acid, 0.1-0.3mg / L indolebutyric acid, 0.05-0.2mg / L salicylic acid, and the pH is controlled to be 5.2-5.6;

[0017] S3. When the leaves grow to more than 30 cm, replace the nutrient solution in the container with distilled water, continue to culture for more than 6 days, and then place it in the culture room to use as a host plant.

[0018] Furthermore, in step S1, after the cutting of the terminal bud is completed, the base of the terminal bud is soaked in 800-1000 times diluted carbendazim for 8-15 minutes.

[0019] Furthermore, a first screen door that can be opened and closed is arranged on one side of the culture chamber, and a second screen door that can be opened and closed is arranged on the other side, and both the first screen door and the second screen door have mesh holes.

[0020] The above-mentioned method for raising the sisal mealybug has the following advantages:

[0021] (1) The present invention selects a suitable host plant, sets a suitable culture temperature, humidity and photoperiod to feed the sisal hemp mealybug, shortens the cultivation period of the sisal hemp mealybug, and improves the survival rate of the sisal hemp mealybug.

[0022] (2) The present invention cultivates host plants in a hydroponic manner, so that the sisal mealybugs can gather more on leaves and stems, which provides convenience for insect inoculation and reduces the possibility of infection due to harmful bacteria in the soil.

[0023] (3) The present invention cultivates pineapples with a suitable nutrient solution so that the pineapples have a higher soluble protein content, thereby better luring the sisal pineapple mealybugs to feed, further shortening the cultivation period of the sisal pineapple mealybugs.

[0024] (4) The present invention also introduces ants, which will carry the sisal pineapple mealybugs to new pineapple plants, thereby ensuring the nutritional supply of the sisal pineapple mealybugs to further improve their survival rate.

[0025] (5) When placing a new hydroponic pineapple, the present invention also hangs a rubber plug soaked with mealybug attractant, thereby attracting the new sisal pineapple mealybugs to transfer to a new healthy host plant, which can better ensure their nutritional supply and further improve their survival rate.

[0026] (6) The culture room is further provided with a first screen door and a second screen door, so that a new host plant can be conveniently placed through one of the screen doors and an old host plant can be conveniently taken out through the other screen door; both the first screen door and the second screen door have mesh holes, so as to promote convection ventilation and ensure a ventilated environment for the breeding of sisal mealybugs. Of course, the size of the mesh holes shall be such that the sisal mealybugs cannot escape.

[0027] (7) The method of the present invention can realize laboratory breeding of the gray mealybug Pseudococcus sisalensis, which is of great significance for the control research of the gray mealybug Pseudococcus sisalensis. DETAILED DESCRIPTION

[0028] In order to make the purpose, technical solution and advantages of the present invention clearer, the technical solution of the present invention will be clearly and completely described below in combination with the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0029] Example 1

[0030] A method for raising mealybugs of sisal hemp comprises the following steps:

[0031] (1) selecting a hydroponic pineapple as a host plant and placing it in a culture room, transferring the female adults of the sisal new pineapple gray mealybug to be born to the hydroponic pineapple, and transferring 8 female adults of the sisal new pineapple gray mealybug to each hydroponic pineapple;

[0032] (2) after the female adult of the sisal new pineapple mealybug produces nymphs, a new hydroponic pineapple is placed every 13 days, the new hydroponic pineapple is placed in a position where the leaves of the new hydroponic pineapple partially overlap with the leaves of the original hydroponic pineapple, and after the new hydroponic pineapple is placed for 2 days, the original hydroponic pineapple is taken out, and the above-mentioned step of replacing the hydroponic pineapple is repeated to carry out successive generation rearing of the sisal new pineapple mealybug;

[0033] In steps (1) and (2), the rearing conditions in the culture room are: temperature 23-25°C, humidity 80-90%, and photoperiod 13L:11D.

[0034] In step (2), when the gray mealybug nymphs of the new sisal pineapple grow to the second age, ants are introduced, and the number of ants introduced to each hydroponic pineapple is 15. When the new hydroponic pineapple is placed, pineapple juice is smeared on the roots of the pineapple leaves, and a rubber plug soaked in mealybug attractant is hung on the top of the hydroponic pineapple.

[0035] The hydroponic pineapple used as a host plant is cultured in advance before being placed in a culture room, and the cultivation steps include: S1. taking a pineapple plant with a strong terminal bud as a seedling, cutting the terminal bud and keeping the base of the terminal bud intact, soaking the base of the terminal bud with a 1000-fold solution of carbendazim for 10 minutes, taking it out and airing it until the cut is dry; S2. putting the air-dried pineapple terminal bud into a disinfected container for hydroponic cultivation, soaking 1 cm of the pineapple terminal bud in water to wait for it to take root, and changing the water every 2 days during the rooting period; when the roots grow to more than 3 cm, putting a nutrient solution in the container to continue hydroponic cultivation, the nutrient solution consists of the following components: 1 / 64MS nutrient solution, 0.2 mg / L boric acid, 0.3 mg / L indolebutyric acid, 0.1 mg / L salicylic acid, and controlling the pH to be 5.5; S3. when the leaves grow to more than 30 cm, replacing the nutrient solution in the container with distilled water, continuing to culture for 7 days, and then putting it into a culture room for use as a host plant.

[0036] A first screen door that can be opened and closed is arranged on one side of the culture room, and a second screen door that can be opened and closed is arranged on the other side. Both the first screen door and the second screen door have mesh holes to prevent the sisal new pineapple gray mealybug from escaping.

[0037] Example 2

[0038] A method for raising mealybugs of sisal hemp comprises the following steps:

[0039] (1) selecting a hydroponic pineapple as a host plant and placing it in a culture room, transferring the female adults of the sisal new pineapple gray mealybug to be born to the hydroponic pineapple, and transferring 6 female adults of the sisal new pineapple gray mealybug to each hydroponic pineapple;

[0040] (2) after the female adult of the sisal new pineapple mealybug produces nymphs, a new hydroponic pineapple is placed every 13 days, the new hydroponic pineapple is placed in a position where the leaves of the new hydroponic pineapple partially overlap with the leaves of the original hydroponic pineapple, and after the new hydroponic pineapple is placed for 2 days, the original hydroponic pineapple is taken out, and the above-mentioned step of replacing the hydroponic pineapple is repeated to carry out successive generation rearing of the sisal new pineapple mealybug;

[0041] In steps (1) and (2), the rearing conditions in the culture room are: temperature 24-26°C, humidity 80-90%, and photoperiod of 12L:12D.

[0042] In step (2), when the gray mealybug nymphs of the new sisal pineapple grow to the second age, ants are introduced, and the number of ants introduced to each hydroponic pineapple is 18. When the new hydroponic pineapple is placed, pineapple juice is smeared on the roots of the pineapple leaves, and a rubber plug soaked in mealybug attractant is hung on the top of the hydroponic pineapple.

[0043] The hydroponic pineapple used as a host plant is cultured in advance before being placed in a culture room, and the cultivation steps include: S1. taking a pineapple plant with a strong terminal bud as a seedling, cutting the terminal bud and keeping the base of the terminal bud intact, soaking the base of the terminal bud with 800 times of carbendazim solution for 8 minutes, taking it out and airing it until the cut is dry; S2. putting the air-dried pineapple terminal bud into a disinfected container for hydroponic cultivation, soaking 1 cm of the pineapple terminal bud in water to wait for it to take root, and changing the water every 2 days during the rooting period; when the roots grow to more than 3 cm, putting a nutrient solution in the container to continue hydroponic cultivation, the nutrient solution consists of the following components: 1 / 32MS nutrient solution, 0.1 mg / L boric acid, 0.1 mg / L indolebutyric acid, 0.2 mg / L salicylic acid, and controlling the pH to be 5.3; S3. when the leaves grow to more than 30 cm, replacing the nutrient solution in the container with distilled water, continuing to culture for 7 days, and then putting it into a culture room for use as a host plant.

[0044] A first screen door that can be opened and closed is arranged on one side of the culture room, and a second screen door that can be opened and closed is arranged on the other side. Both the first screen door and the second screen door have mesh holes to prevent the sisal new pineapple gray mealybug from escaping.

[0045] Example 3

[0046] A method for raising mealybugs of sisal hemp comprises the following steps:

[0047] (1) selecting a hydroponic pineapple as a host plant and placing it in a culture room, transferring the female adults of the sisal new pineapple gray mealybug to be born to the hydroponic pineapple, and transferring 8 female adults of the sisal new pineapple gray mealybug to each hydroponic pineapple;

[0048] (2) after the female adult of the sisal new pineapple mealybug produces nymphs, a new hydroponic pineapple is placed every 10 days, the new hydroponic pineapple is placed in a position where the leaves of the new hydroponic pineapple partially overlap with the leaves of the original hydroponic pineapple, and after the new hydroponic pineapple is placed for 2 days, the original hydroponic pineapple is taken out, and the above-mentioned step of replacing the hydroponic pineapple is repeated to carry out successive generation rearing of the sisal new pineapple mealybug;

[0049] In steps (1) and (2), the rearing conditions in the culture room are: temperature 23-25°C, humidity 80-90%, and photoperiod 13L:11D.

[0050] In step (2), when a new hydroponic pineapple is placed, a rubber plug soaked with mealybug attractant is hung on the top of the hydroponic pineapple.

[0051] The hydroponic pineapple used as a host plant is cultivated in advance before being placed in a culture room, and the cultivation steps include: S1. taking a pineapple plant with a strong terminal bud as a seedling, cutting the terminal bud and keeping the base of the terminal bud intact, and airing it until the incision is dry; S2. placing the air-dried pineapple terminal bud into a sterilized container for hydroponic cultivation, immersing 1 cm of the pineapple terminal bud in water to wait for it to take root, and changing the water every 2 days during the rooting period; when the roots grow to more than 3 cm, putting a nutrient solution in the container to continue hydroponic cultivation, the nutrient solution consists of the following components: 1 / 64MS nutrient solution, 0.1 mg / L boric acid, 0.3 mg / L indolebutyric acid, 0.05 mg / L salicylic acid, and controlling the pH to be 5.5; S3. when the leaves grow to more than 30 cm, replacing the nutrient solution in the container with distilled water, continuing to culture for 7 days, and then placing it in a culture room for use as a host plant.

[0052] A first screen door that can be opened and closed is arranged on one side of the culture room, and a second screen door that can be opened and closed is arranged on the other side. Both the first screen door and the second screen door have mesh holes to prevent the sisal new pineapple gray mealybug from escaping.

[0053] Comparative Example 1

[0054] Comparative Example 1 is substantially the same as Example 1, but when cultivating the host plant, in step S2, when the root grows to more than 3 cm, the nutrient solution is not replaced, and the hydroponic culture is continued with distilled water.

[0055] Comparative Example 2

[0056] Comparative Example 1 is substantially the same as Example 1, but when cultivating the host plant, in step S2, the nutrient solution is 1 / 64MS nutrient solution.

[0057] Comparative Example 3

[0058] Comparative Example 1 is substantially the same as Example 1, but when cultivating the host plant, in step S2, the nutrient solution is 1 / 64MS nutrient solution and 0.3 mg / L indolebutyric acid.

[0059] Comparative Example 4

[0060] Comparative Example 1 is substantially the same as Example 1, except that the nutrient solution is not replaced with distilled water before and after the host plant is placed in the culture chamber.

[0061] In the above-mentioned Examples 1-3 and Comparative Examples 1-3, the soluble protein content of the leaves of the hydroponic pineapples was investigated when they grew to 30 cm and then continued to be cultured for 7 days. The results are shown in Table 1:

[0062] Table 1 Soluble protein content in pineapple leaves

[0063]

[0064] The egg laying amount of female adults of Psoralea sisalensis in the above Examples 1-3 and Comparative Examples 1-4, as well as the survival rate of 1st instar nymphs after 10 days, were investigated. The results are shown in Table 2:

[0065] Table 2 Survey on the egg laying of female adults and the survival rate of first-instar nymphs of Psoralea sisalensis

[0066] Egg laying amount (grains) Survival rate (%) Example 1 219.9±12.5 90.1±6.7 Example 2 202.8±8.7 78.2±5.8 Example 3 215.2±11.6 89.5±7.2 Comparative Example 1 81.3±10.1 50.1±8.2 Comparative Example 2 151.6±13.8 76.1±6.9 Comparative Example 3 160.3±12.7 76.0±8.5 Comparative Example 4 139.1±15.3 69.1±7.8

[0067] The development period of female nymphs of the sisal new pineapple mealybug in the above Examples 1-3 and Comparative Examples 1-4 was investigated, and the results are shown in Table 3:

[0068] Table 3 Investigation of the developmental period of female nymphs of the sisal mealybug

[0069]

[0070]

Claims

1. A method for raising mealybugs of sisal hemp, Features The following steps are involved: (1) Selecting a hydroponic pineapple as a host plant and placing it in a culture room, transferring the female adults of the sisal pineapple mealybug ready to give birth to the hydroponic pineapple; (2) After the female adult of the sisal new pineapple mealybug produces nymphs, a new hydroponic pineapple is placed every 10-15 days, and the new hydroponic pineapple is placed in a position where the leaves partially overlap with the leaves of the original hydroponic pineapple. After the new hydroponic pineapple is placed for 1-2 days, the original hydroponic pineapple is taken out, and the above steps of replacing the hydroponic pineapple are repeated to carry out subgeneration rearing of the sisal new pineapple mealybug; In steps (1) and (2), the rearing conditions in the culture room are: temperature 22-26°C, humidity 80-90%, and photoperiod of (11-13)L:(13-11)D.

2. The breeding method of the sisal mealybug according to claim 1, Features: In step (2), when the sisal pineapple mealybug nymphs grow to the second instar, ants are introduced, and the number of ants introduced to each hydroponic pineapple is 10-20.

3. The breeding method of the sisal mealybug according to claim 1, Features: In step (1), 6-10 female adults of the sisal new pineapple mealybug are transferred to each hydroponic pineapple plant.

4. The breeding method of the sisal mealybug according to claim 1, Features: In step (2), when a new hydroponic pineapple is placed, a rubber plug soaked in mealybug attractant is hung on the top of the hydroponic pineapple.

5. The breeding method of the sisal mealybug according to claim 2, Features: In step (2), after the ants are implanted, when a new hydroponic pineapple is placed, pineapple juice is applied to the base of the pineapple leaves.

6. The breeding method of the sisal mealybug according to claim 1, Features: Before the hydroponic pineapple is placed in the culture room, it is pre-cultivated. The cultivation steps include: S1. Take pineapple plants with strong terminal buds as seedlings, cut the terminal buds and keep the base of the terminal buds intact, and dry them in the sun until the cut is dry; S2. Place the air-dried pineapple terminal buds into a sterilized container for hydroponics, soak the pineapple terminal buds in water for 0.5-1 cm to wait for rooting, and change the water every 2-3 days during the rooting period; wait for the roots to grow to more than 3 cm, place a nutrient solution in the container and continue hydroponics, the nutrient solution is composed of the following ingredients: 1 / 32-1 / 64MS nutrient solution, 0.1-0.3 mg / L boric acid, 0.1-0.3 mg / L indolebutyric acid, 0.05-0.2 mg / L salicylic acid, and the pH is controlled to be 5.2-5.6; S3. When the leaves grow to more than 30 cm, replace the nutrient solution in the container with distilled water, continue to cultivate for more than 6 days, and then place it in the culture room to use as a host plant.

7. The breeding method of the sisal mealybug according to claim 1, Features: In step S1, after the cutting of the terminal bud is completed, the base of the terminal bud is soaked in 800-1000 times dilution of carbendazim for 8-15 min.

8. The breeding method of the sisal mealybug according to claim 1, Features: A first screen door that can be opened and closed is arranged on one side of the culture chamber, and a second screen door that can be opened and closed is arranged on the other side, and both the first screen door and the second screen door have mesh holes.

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