Composition containing probiotics and chlorella and preparation method thereof

Abstract

The invention relates to the field of probiotic foods, in particular to a composition containing probiotics and chlorella and a preparation method of the composition. The composition is prepared from the following raw materials: lactobacillus rhamnosus, bifidobacterium longum, lactobacillus johnsonii, a chlorella extract, a rhizoma polygonati extract, sugar alcohol, gelatin, chitosan, resistant dextrin and water. The composition prepared by matching compound probiotics lactobacillus rhamnosus, bifidobacterium longum and lactobacillus johnsonii with prebiotics such as a chlorella extract, a rhizoma polygonati extract and sugar alcohol by utilizing gelatin, chitosan and resistant dextrin can be applied to functional food for regulating the intestinal flora structure, so that the problem of imbalance of the intestinal flora is solved.

Description

Technical Field

[0001] The invention relates to the field of probiotic foods, and in particular to a composition containing probiotics and chlorella and a preparation method thereof. Background Art

[0002] The intestine is considered the "second brain" of human health, and its microbial composition and function are crucial to human health. 14 The intestinal flora is a complex ecosystem composed of microorganisms that form a symbiotic relationship with the human body. The intestinal flora is known as an in vitro organ. It not only plays an important role in food digestion and absorption, but also participates in the maintenance of the body's health. Intestinal flora imbalance is closely related to the occurrence of diseases. However, modern lifestyles and unreasonable dietary structures have made intestinal flora imbalance a common phenomenon. In addition, problems such as intestinal inflammation and decreased immune function often plague people. Therefore, it is particularly important to find a safe and effective way to maintain intestinal health.

[0003] Probiotics are one or several types of bacterial preparations that are beneficial to the intestines, that is, the beneficial bacteria in our intestines. Since harmful bacteria are mostly responsible for the disease state, the increase in the number of beneficial bacteria helps to restore the balance of the intestinal flora structure, so that the intestinal microecology can be re-established, thereby promoting intestinal health. In addition, probiotics can affect the secretion of microorganisms and host metabolites in the intestine, reducing and alleviating the possibility of intestinal diseases. Therefore, the rational selection and use of probiotics is of great significance to maintaining intestinal health. However, in the process of handling and storing probiotics, some factors will reduce the activity of probiotics, such as pH, oxygen and temperature. When probiotics pass through the human gastrointestinal tract, they need to pass through digestive organs such as the stomach and small intestine. The gastric acid, bile, digestive enzymes, etc. in them reduce their vitality during the transportation to the target site of the gastrointestinal tract, making it difficult to efficiently colonize in the intestine, so the probiotic effect cannot be achieved or is poor, resulting in the restriction of the application of probiotics.

[0004] Therefore, it is urgent to develop a composition containing probiotics and maintaining the activity of probiotics to promote the application of probiotics. Summary of the invention

[0005] In order to solve the above technical problems, the present invention provides a composition containing probiotics and Chlorella and a preparation method thereof. The composition is prepared by using gelatin, chitosan, and resistant dextrin to combine composite probiotics Lactobacillus rhamnosus, Bifidobacterium longum and Lactobacillus johnsonii with Chlorella extract, Polygonatum sibiricum extract, sugar alcohol and other prebiotics, which can regulate the structure of intestinal flora and thus solve the problem of intestinal flora imbalance.

[0006] In order to achieve the above object, the present invention adopts the following technical solutions:

[0007] A composition containing probiotics and chlorella extract, wherein the raw materials of the composition include, by weight, 4-10 parts of composite probiotics, 12-26 parts of prebiotics, 55-85 parts of preparation auxiliary materials and water.

[0008] The compound probiotics are compound probiotics of Lactobacillus rhamnosus, Bifidobacterium longum and Lactobacillus johnsonii.

[0009] Preferably, Lactobacillus rhamnosus ≥ 1.0*10 6 cuf / g, Bifidobacterium longum≥1.0*10 6 cuf / g, Lactobacillus johnsonii≥1.0*10 6 cuf / g.

[0010] Preferably, the mass ratio of the composite probiotics Lactobacillus rhamnosus: Bifidobacterium longum: Lactobacillus johnsonii is 2-3:1:1.

[0011] The prebiotics are chlorella extract, polygonatum extract and sugar alcohol.

[0012] The chlorella extract is chlorella polysaccharide.

[0013] The polygonatum sibiricum extract is a polygonatum sibiricum water extract.

[0014] The sugar alcohol is one or more of sorbitol and maltitol.

[0015] More preferably, the sugar alcohol is maltitol.

[0016] Preferably, the mass ratio of the Chlorella extract, the Polygonatum sibiricum extract and the sugar alcohol in the prebiotics is 1-5:1:10-20.

[0017] The preparation auxiliary materials are gelatin, chitosan and resistant dextrin.

[0018] Preferably, the mass ratio of the preparation excipients gelatin, chitosan and resistant dextrin is 5:0.5-2.5:10-20.

[0019] Preferably, the raw materials of the composition include: Lactobacillus rhamnosus, Bifidobacterium longum and Lactobacillus johnsonii, Chlorella extract, Polygonatum sibiricum extract, sugar alcohol, gelatin, chitosan, resistant dextrin and water.

[0020] Preferably, the mass parts of raw water in the composition can be 20-150 parts.

[0021] The composition can be applied to functional foods to prepare various preparations, including but not limited to pills, tablets, capsules, powders, granules, granules, etc.

[0022] The application of the composition containing probiotics and chlorella extract in products for regulating the intestinal tract.

[0023] The present invention also provides a method for preparing the above-mentioned composition containing probiotics and chlorella, comprising the following steps:

[0024] S1: preparing polygonatum extract: crushing dried polygonatum, sieving through 200 mesh, adding polygonatum powder and water in a mass ratio of 1:30-50, extracting at 70-80° C. for 1-2 hours, centrifuging, filtering, and concentrating the filtrate to a relative density of 1.1-1.2 to obtain polygonatum extract for later use;

[0025] S2 mixing: weigh corresponding Lactobacillus rhamnosus, Bifidobacterium longum and Lactobacillus johnsonii, Chlorella extract, Polygonatum sibiricum extract and sugar alcohol according to mass fractions and mix them evenly to obtain probiotics-prebiotics mixed particles for standby use;

[0026] S3 preparation: Weigh the corresponding probiotic-prebiotic mixed granules and gelatin, chitosan, resistant dextrin and water according to their mass fractions and mix them to obtain a composition containing probiotics and Chlorella extract.

[0027] The beneficial effects of the present invention are:

[0028] 1. The present invention uses three probiotics, namely Lactobacillus rhamnosus, Bifidobacterium longum and Lactobacillus johnsonii, as core effective ingredients, and regulates the imbalance of intestinal flora through the synergistic effect between the probiotics.

[0029] 2. The present invention compounds prebiotics such as chlorella extract, polygonatum extract, and sugar alcohol into probiotics, which can promote the growth and proliferation of probiotics and then quickly regulate the structure of intestinal flora.

[0030] 3. The present invention utilizes gelatin, chitosan, and resistant dextrin to encapsulate the active ingredients to prepare functional foods, which can effectively maintain the activity and stability of probiotics. DETAILED DESCRIPTION

[0031] The technical solution of the present invention is further described below in conjunction with specific examples. In the following examples, Lactobacillus rhamnosus, Bifidobacterium longum, and Lactobacillus johnsonii were all purchased from Shanxi Linwei Biotechnology Co., Ltd. Chlorella polysaccharide was purchased from Shandong Weitai Bioengineering Co., Ltd. Polygonatum sibiricum was purchased from Shandong Weitai Bioengineering Co., Ltd. Other raw materials used, unless otherwise specified, can be obtained from conventional commercial channels; the processes used, unless otherwise specified, all adopt conventional processes in the art.

[0032] Example 1

[0033] A method for preparing a composition containing probiotics and chlorella.

[0034] S1: preparing polygonatum sibiricum extract: crushing dried polygonatum sibiricum, sieving through 200 mesh, adding polygonatum sibiricum powder and water in a mass ratio of 1:50, extracting at 80° C. for 2 h, centrifuging, filtering, and concentrating the filtrate to a relative density of 1.2 to obtain polygonatum sibiricum extract for later use;

[0035] S2 mixing: weigh 4 parts of Lactobacillus rhamnosus, 2.5 parts of Bifidobacterium longum, 2.5 parts of Lactobacillus johnsonii, 3 parts of Chlorella extract, 1 part of Polygonatum sibiricum extract, and 15 parts of maltitol according to mass fractions, mix well, and obtain probiotic prebiotic mixed particles for standby use;

[0036] S3 preparation: 5 parts of gelatin, 1.5 parts of chitosan, 15 parts of resistant dextrin, probiotic-prebiotic mixed granules and water (added to 100 parts) were weighed according to their mass parts and mixed to obtain a composition containing probiotics and chlorella extract.

[0037] Example 2

[0038] A method for preparing a composition containing probiotics and chlorella.

[0039] S1: preparing polygonatum sibiricum extract: crushing dried polygonatum sibiricum, sieving through 200 mesh, adding polygonatum sibiricum powder and water in a mass ratio of 1:30, extracting at 75°C for 1.5h, centrifuging, filtering, and concentrating the filtrate to a relative density of 1.1 to obtain polygonatum sibiricum extract for later use;

[0040] S2 mixing: weigh 2 parts of Lactobacillus rhamnosus, 1 part of Bifidobacterium longum, 1 part of Lactobacillus johnsonii, 1 part of Chlorella extract, 1 part of Polygonatum sibiricum extract, and 10 parts of maltitol according to mass fractions, mix well, and obtain probiotic prebiotic mixed particles for standby use;

[0041] S3 preparation: 5 parts of gelatin, 0.5 parts of chitosan, 20 parts of resistant dextrin, probiotic and prebiotic mixed granules and water (added to 100 parts) were weighed according to their mass parts and mixed to obtain a composition containing probiotics and chlorella extract.

[0042] Example 3

[0043] A method for preparing a composition containing probiotics and chlorella.

[0044] S1: preparing polygonatum extract: crushing dried polygonatum, sieving through 200 mesh, adding polygonatum powder and water in a mass ratio of 1:40, extracting at 70° C. for 1 h, centrifuging, filtering, and concentrating the filtrate to a relative density of 1.2 to obtain polygonatum extract for later use;

[0045] S2 mixing: weigh 6 parts of Lactobacillus rhamnosus, 2 parts of Bifidobacterium longum, 2 parts of Lactobacillus johnsonii, 5 parts of Chlorella extract, 1 part of Polygonatum sibiricum extract, and 20 parts of maltitol according to mass fractions, mix well, and obtain probiotic prebiotic mixed particles for standby use;

[0046] S3 preparation: 5 parts of gelatin, 2.5 parts of chitosan, 10 parts of resistant dextrin, probiotic-prebiotic mixed granules and water (added to 100 parts) were weighed according to their mass parts and mixed to obtain a composition containing probiotics and chlorella extract.

[0047] The formula composition of a composition containing probiotics and Chlorella in Examples 1-3 is shown in Table 1.

[0048] Table 1 Composition formula containing probiotics and chlorella

[0049]

[0050]

[0051] Note: Lactobacillus rhamnosus ≥ 1.0*10 6 cuf / g, Bifidobacterium longum≥1.0*10 6 cuf / g, Lactobacillus johnsonii≥1.0*10 6 cuf / g.

[0052] Comparative Examples 1-4

[0053] A method for preparing a functional food containing probiotics is as follows:

[0054] S1: preparing polygonatum sibiricum extract: crushing dried polygonatum sibiricum, sieving through 200 mesh, adding polygonatum sibiricum powder and water in a mass ratio of 1:50, extracting at 80° C. for 2 h, centrifuging, filtering, and concentrating the filtrate to a relative density of 1.2 to obtain polygonatum sibiricum extract for later use;

[0055] S2 mixing: weigh corresponding Lactobacillus rhamnosus, Bifidobacterium longum and Lactobacillus johnsonii, Chlorella extract, Polygonatum sibiricum extract and sugar alcohol according to mass fractions and mix them evenly to obtain probiotics-prebiotics mixed particles for standby use;

[0056] S3 preparation: Weigh the corresponding probiotic-prebiotic mixed granules and gelatin, chitosan, resistant dextrin and water according to their mass fractions and mix them to obtain a composition containing probiotics and Chlorella extract.

[0057] The difference from Example 2 is the formula composition of a composition containing probiotics in Comparative Examples 1-4, as shown in Table 2.

[0058] Table 2 Composition formula containing probiotics and chlorella

[0059]

[0060]

[0061] Note: Lactobacillus rhamnosus ≥ 1.0*106 cuf / g, Bifidobacterium longum≥1.0*10 6 cuf / g, Lactobacillus johnsonii≥1.0*10 6 cuf / g.

[0062] Effect test 1

[0063] The gastric acid resistance of a functional food containing probiotics and chlorella in Examples 1-3 of the present invention and a functional food containing probiotics in Comparative Examples 1-4 was tested by simulating worker gastric acid. The combination containing probiotics and chlorella in each embodiment and comparative example was mixed with water in a ratio of 1:2 and shaken well, and the initial inoculation amount of Lactobacillus rhamnosus, Bifidobacterium longum, and Lactobacillus johnsonii was determined. Then, they were added to simulated gastric fluid and simulated intestinal fluid for treatment for 3 hours, and the residual bacteria amount of Lactobacillus rhamnosus, Bifidobacterium longum, and Lactobacillus johnsonii after the simulated fluid treatment was determined. Then the survival rate of the probiotics was calculated, and the test results are shown in Tables 3 and 4.

[0064] Probiotic survival rate = (initial inoculation amount - remaining bacteria amount) / initial inoculation amount * 100%.

[0065] Table 3 Survival rate of probiotics in simulated gastric fluid

[0066]

[0067]

[0068] Table 4 Survival rate of probiotics in simulated intestinal fluid

[0069] Experimental Group Lactobacillus rhamnosus Bifidobacterium longum Lactobacillus johnsonii Example 1 150.4％ 170.8％ 148.8％ Example 2 130.5％ 157.7％ 130.5％ Example 3 128.9％ 160.2％ 123.6％ Comparative Example 1 101.5％ 90.2％ 100.9％ Comparative Example 2 108.3％ 100.5％ 110.1％ Comparative Example 3 103.2％ 99.8％ 105.5％ Comparative Example 4 102.5％ 104.0％ 100.4％

[0070] Effect test 2

[0071] The samples of Example 2 and Comparative Example 1 were used to perform an animal experiment of intragastric administration on mice with an imbalanced intestinal flora model to further illustrate the efficacy of the product of the present invention, as follows:

[0072] After 1 week of adaptive feeding of ICR mice, and 7 days of adaptive feeding of ICR mice (18-22g), they were randomly divided into 4 groups (normal group, blank group, sample group of Example 1, sample group of Comparative Example 1) according to body weight, with 10 mice in each group. Except for the normal group, mice in other groups were gavaged with 22.4g / kg (mouse weight) of ampicillin for 3 days every day, with each dose of 11.2g / kg, 2 times / d; mice in the normal group were gavaged with the same volume of physiological saline at the same time every day as a reference. The feces of mice in each group were quickly and aseptically collected on days 0, 3, 5, and 7 of administration. After preliminary identification by colony characteristics, Gram staining, microscopy, etc., the counts were made, and the total number of lactobacillus colonies per gram of wet feces was calculated. The results are shown in Table 5.

[0073] Table 5 Total number of lactobacilli colonies per gram of wet feces

[0074]

[0075] The above embodiments are preferred implementation modes of the present invention, but the implementation modes of the present invention are not limited to the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications that do not deviate from the spirit and principles of the present invention should be equivalent replacement methods and are included in the protection scope of the present invention.

Claims

1. A composition containing probiotics and chlorella extract, characterized in that: The raw materials of the functional food include, by weight: 4-10 parts of compound probiotics, 12-26 parts of prebiotics, 55-85 parts of preparation excipients and water; The composite probiotics are composite probiotics of Lactobacillus rhamnosus, Bifidobacterium longum and Lactobacillus johnsonii; The prebiotics are chlorella extract, polygonatum extract, and sugar alcohol; The preparation auxiliary materials are gelatin, chitosan and resistant dextrin.

2. The composition according to claim 1, characterized in that The mass ratio of the composite probiotics Lactobacillus rhamnosus: Bifidobacterium longum: Lactobacillus johnsonii is 2-3:1:

1.

3. The composition according to claim 1, characterized in that The sugar alcohol is one or more of sorbitol and maltitol.

4. The composition according to claim 1, characterized in that The mass ratio of the chlorella extract, the polygonatum extract and the sugar alcohol in the prebiotics is 1-5:1:10-20.

5. The composition according to claim 1, characterized in that The mass ratio of the preparation auxiliary materials gelatin, chitosan and resistant dextrin is 5:0.5-2.5:10-20.

6. The composition according to claim 1, characterized in that The chlorella extract is chlorella polysaccharide.

7. The composition according to claim 3, characterized in that The polygonatum sibiricum extract is a polygonatum sibiricum water extract.

8. The composition according to claim 1, characterized in that The raw materials of the functional food include, by weight: 2-6 parts of Lactobacillus rhamnosus, 1-2.5 parts of Bifidobacterium longum, 1-2.5 parts of Lactobacillus johnsonii, 1-5 parts of Chlorella extract, 0.5-1.5 parts of Polygonatum sibiricum extract, 10-20 parts of sugar alcohol, 5-6 parts of gelatin, 0.5-2.5 parts of chitosan, 10-20 parts of resistant dextrin and water.

9. A method for preparing the composition according to any one of claims 1 to 8, characterized in that: The following steps are involved: S1: preparing polygonatum extract: crushing dried polygonatum, sieving through 200 mesh, adding polygonatum powder and water in a mass ratio of 1:30-50, extracting at 70-80° C. for 1-2 hours, centrifuging, filtering, and concentrating the filtrate to a relative density of 1.1-1.2 to obtain polygonatum extract for later use; S2 mixing: weigh Lactobacillus rhamnosus, Bifidobacterium longum and Lactobacillus johnsonii, Chlorella extract, Polygonatum sibiricum extract and sugar alcohol according to mass fractions, mix them evenly to obtain probiotics-prebiotics mixed particles for standby use; S3 preparation: Weigh the probiotic-prebiotic mixed granules and gelatin, chitosan, resistant dextrin and water according to their mass fractions and mix them to obtain a composition containing probiotics and Chlorella extract.

10. The composition according to any claim 1, characterized in that The composition is applied to functional foods to prepare various preparations, including but not limited to tablets, capsules, powders, granules or granules.

11. Use of the composition according to any one of claims 1 to 10 or the composition prepared by the preparation method according to claim 9 in a product for regulating the intestinal tract.

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