Biological peptide antibacterial tablet and preparation method thereof

Abstract

The invention provides a biological peptide bacteriostatic tablet and a preparation method thereof. The bletilla striata extract and the white peony root extract are prepared through a simple, convenient and energy-saving extraction method, the bletilla striata extract and the white peony root extract are compounded with the biological peptide and the chlorhexidine acetate according to a specific proportion, the antibacterial activity of the composition is remarkably improved, the dissolution speed of the biological peptide antibacterial tablet is increased through the formula design and the preparation method matched with the composition, and the use feeling is optimized.

Description

Technical Field

[0001] The invention belongs to the field of medicine, and specifically relates to a biopeptide antibacterial tablet and a preparation method thereof. Background Art

[0002] Biopeptide antibacterial tablets are a type of health product or medicine that uses biopeptides as the main active ingredient, aiming to use the natural antibacterial properties of peptides to achieve the purpose of antibacterial and health maintenance. Biopeptides are short-chain molecules composed of amino acids, which play a variety of roles in biology, including but not limited to signal transduction, immune regulation, cell proliferation and differentiation.

[0003] Bletilla striata extract is a natural product extracted from the traditional Chinese medicine Bletilla striata (scientific name: Bletilla striata). It mainly contains bletilla polysaccharides, ferulic acid, chlorogenic acid, flavonoids, steroidal saponins and other active substances. It has antibacterial and anti-inflammatory, antioxidant and skin repair effects.

[0004] White peony extract is a natural product extracted from the traditional Chinese medicine white peony (Cynanchum Otophyllum). Its main active ingredients include paeoniflorin, volatile oils, tannins, flavonoids and various organic acids. It has the effects of regulating immunity, anti-inflammatory and analgesic, and antioxidant.

[0005] Currently, there are biopeptide antibacterial tablets on the market for female genitals to prevent inflammation and inhibit bacteria, but their functions are relatively single and cannot achieve a broad-spectrum antibacterial effect. Bletilla striata extract and white peony extract contain a large number of active substances with excellent antibacterial and anti-inflammatory effects, and have received widespread attention for their synergistic antibacterial effects with biopeptides, but the existing extract products have poor activity and limited antibacterial effects. Therefore, a biopeptide antibacterial tablet product with good stability and excellent antibacterial effect has a wide market demand. Summary of the invention

[0006] The purpose of the present invention is to provide a biopeptide antibacterial tablet with good stability and excellent antibacterial effect and a preparation method thereof.

[0007] In order to achieve the above object, the present invention provides the following technical solutions: A biopeptide antibacterial tablet, the components of which include Bletilla Striata extract, White Peony (Cynanchum Otophyllum) extract, biopeptide, collagen, sodium hyaluronate, diguanosine tetraphosphate, chlorhexidine acetate and excipients.

[0008] Preferably, the mass fractions of the components are 4-8% of Bletilla striata extract, 6-8% of White Peony Root extract, 2-3% of biological peptides, 5-10% of collagen, 12-18% of sodium hyaluronate, 0.02-0.2% of diguanosine tetraphosphate, and 4-8% of chlorhexidine acetate.

[0009] Preferably, calculated by mass percentage of the bio-peptide antibacterial tablets, the auxiliary materials include 2-5% isomaltooligosaccharide, 1-2% magnesium stearate, 20-30% cyclodextrin, and the balance is starch.

[0010] Preferably, the Bletilla striata extract is prepared by the following method: M1. Crush the tubers of Bletilla striata, extract with hot water for 5-10 hours, and filter to obtain the extract and residue; M2. Add 70-95% ethanol solution to the residue, stir and continue to extract in an ultrasonic water bath for 1-3 hours, filter with suction, and mix the filtrate with the extract obtained in step M1 to obtain a Bletilla striata extract; M3. The Bletilla striata extract is centrifuged, concentrated under reduced pressure, and then freeze-dried to obtain the Bletilla striata extract.

[0011] Preferably, the temperature of hot water extraction in step M1 is 60-75° C., and the mass ratio of water to crushed Bletilla striata tubers is 2-6:1.

[0012] Optionally, in the M1 step, the mass ratio of water to the crushed Bletilla striata tubers is 2:1, 3:1, 4:1, 5:1 or 6:1.

[0013] Preferably, the water bath temperature in the M2 step is 35-45° C., and the volume ratio of the ethanol solution to the filter residue is 1.5-3:1.

[0014] Further preferably, the mass concentration of the ethanol solution in the M2 step is 80% to 90%.

[0015] Preferably, the ultrasonic power in the M2 step is 500-1000W.

[0016] Preferably, the white peony root extract is prepared by the following method: S1. Crush the white peony root, add acid and cook for 1-2h, add water, and cool to below 45°C to obtain white peony wet material; S2. Add cellulase and protease to the white peony wet material, and keep warm for enzymolysis for 1-2 hours; S3. The enzymatic hydrolysis product is centrifuged and filtered, and the filter residue is washed with an ethanol solution. The washing solution is combined with the filtrate to obtain a white peony extract, and the white peony extract is evaporated, concentrated, and dried to obtain a white peony extract.

[0017] Preferably, the acid in step S1 is hydrochloric acid or citric acid solution, with a concentration of 0.1-1 wt %, and a mass ratio of acid to white peony root of 1.5-2:1.

[0018] Preferably, the temperature of acid leaching in step S1 is 70-90°C.

[0019] Preferably, the amount of water added in step S1 is 1.5 to 3 times the volume of the mixture after acid leaching.

[0020] Preferably, the added amount of the cellulase is 2-5% of the weight of the white peony root, and the added amount of the protease is 1-3% of the weight of the white peony root.

[0021] The present invention also provides a method for preparing the above-mentioned biopeptide antibacterial tablets, comprising the following steps: uniformly mixing bletilla striata extract, white peony extract, biopeptide, collagen, sodium hyaluronate, diguanosine tetraphosphate, chlorhexidine acetate and auxiliary materials, compressing granules, and tableting.

[0022] Preferably, during the mixing, the bletilla striata extract, white peony extract, biopeptide and chlorhexidine acetate are first mixed evenly, and then collagen, sodium hyaluronate and diguanosine tetraphosphate are mixed, and then mixed with the aforementioned mixture, and oligosaccharide, cyclodextrin and starch are added and mixed, and finally magnesium stearate is added.

[0023] Compared with the prior art, the advantages and beneficial effects of the present invention are as follows: the present invention provides a new biopeptide antibacterial tablet and a preparation method thereof, the present invention extracts bletilla striata extract and white peony root extract by a self-developed method, and compounding the extract with biopeptide and chlorhexidine acetate in a specific ratio, synergistically enhancing the antibacterial activity of the composition; and the preparation method of bletilla striata extract and white peony root extract in the present invention has simple steps, reduces energy consumption by temperature control and rational use of heat, and has industrial progressive significance. In addition, the biopeptide antibacterial tablet prepared by the formula and method of the present invention has good stability and dissolution rate, and is more convenient to use. DETAILED DESCRIPTION

[0024] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0025] Example 1 The present embodiment provides a biopeptide antibacterial tablet, whose components include 4% of bletilla striata extract, 8% of white peony root extract, 2% of biopeptide, 6% of collagen, 15% of sodium hyaluronate, 0.05% of diguanosine tetraphosphate, 5% of chlorhexidine acetate, 2% of isomaltooligosaccharide, 1% of magnesium stearate, 24% of cyclodextrin, and the balance is starch.

[0026] The bletilla striata extract is prepared by the following method: M1. Crush 100g of Bletilla striata tubers, add 300g of water, heat to 65℃ and extract for 8h, filter to obtain the extract and residue; M2. Add 80% ethanol solution twice the volume of the residue to the residue, stir and continue to extract in an ultrasonic water bath for 2h, the water bath temperature is 40 ° C, the ultrasonic power is 1000W, filter, and mix the filtrate with the extract obtained in step M1 to obtain a Bletilla striata extract; M3. The Bletilla striata extract is centrifuged, concentrated under reduced pressure, and then freeze-dried to obtain the Bletilla striata extract.

[0027] The white peony root extract is prepared by the following method: S1. 100g of white peony root was crushed, 200g of 0.2wt% aqueous hydrochloric acid solution was added and boiled at 80 ℃ for 1h, and 2 times the volume of water was added after acid boiling, and cooled to 45 ℃ to obtain white peony wet material; S2. Add 3g of cellulase and 1g of protease to the wet material of white peony root, and keep it at 45℃ for 1.5h; S3. The enzymatic hydrolysis product is centrifuged and filtered, and the filter residue is washed with an ethanol solution. The washing solution is combined with the filtrate to obtain a white peony extract, and the white peony extract is evaporated, concentrated, and dried to obtain a white peony extract.

[0028] This embodiment also provides a method for preparing the above-mentioned biopeptide antibacterial tablets, wherein the extract of Bletilla striata, the extract of Paeonia lactiflora, the biopeptide and chlorhexidine acetate are weighed and mixed evenly by mass percentage, and collagen, sodium hyaluronate and diguanosine tetraphosphate are mixed, and then mixed with the above-mentioned mixture, and oligosaccharide, cyclodextrin and starch are added and mixed, and finally magnesium stearate is added. After the above-mentioned materials are mixed evenly, they are compressed and granulated into tablets of 0.25 g / tablet.

[0029] Example 2 The present embodiment provides a biopeptide antibacterial tablet, whose components include 6% of bletilla striata extract, 6% of white peony root extract, 2% of biopeptide, 5% of collagen, 15% of sodium hyaluronate, 0.05% of diguanosine tetraphosphate, 5% of chlorhexidine acetate, 2% of isomaltooligosaccharide, 1% of magnesium stearate, 24% of cyclodextrin, and the balance is starch.

[0030] The bletilla striata extract is prepared by the following method: M1. Crush 100g of Bletilla striata tubers, add 300g of water, heat to 65℃ and extract for 8h, filter to obtain the extract and residue; M2. Add 80% ethanol solution twice the volume of the residue to the residue, stir and continue to extract in an ultrasonic water bath for 2h, the water bath temperature is 40 ° C, the ultrasonic power is 1000W, filter, and mix the filtrate with the extract obtained in step M1 to obtain a Bletilla striata extract; M3. The Bletilla striata extract is centrifuged, concentrated under reduced pressure, and then freeze-dried to obtain the Bletilla striata extract.

[0031] The white peony root extract is prepared by the following method: S1. 100g of white peony root was crushed, 200g of 0.2wt% aqueous hydrochloric acid solution was added and boiled at 80 ℃ for 1h, and 2 times the volume of water was added after acid boiling, and cooled to 45 ℃ to obtain white peony wet material; S2. Add 3g of cellulase and 1g of protease to the wet material of white peony root, and keep it at 45℃ for 1.5h; S3. The enzymatic hydrolysis product is centrifuged and filtered, and the filter residue is washed with an ethanol solution. The washing solution is combined with the filtrate to obtain a white peony extract, and the white peony extract is evaporated, concentrated, and dried to obtain a white peony extract.

[0032] This embodiment also provides a method for preparing the above-mentioned biopeptide antibacterial tablets, wherein the extract of Bletilla striata, the extract of Paeonia lactiflora, the biopeptide and chlorhexidine acetate are weighed and mixed evenly by mass percentage, and collagen, sodium hyaluronate and diguanosine tetraphosphate are mixed, and then mixed with the above-mentioned mixture, and oligosaccharide, cyclodextrin and starch are added and mixed, and finally magnesium stearate is added. After the above-mentioned materials are mixed evenly, they are compressed and granulated into tablets of 0.25 g / tablet.

[0033] Example 3 The present embodiment provides a biopeptide antibacterial tablet, whose components include 6% of bletilla striata extract, 6% of white peony root extract, 2% of biopeptide, 5% of collagen, 15% of sodium hyaluronate, 0.05% of diguanosine tetraphosphate, 5% of chlorhexidine acetate, 2% of isomaltooligosaccharide, 1% of magnesium stearate, 24% of cyclodextrin, and the balance is starch.

[0034] The bletilla striata extract is prepared by the following method: M1. Crush 100g of Bletilla striata tubers, add 300g of water, heat to 70℃ and extract for 7h, filter to obtain the extract and residue; M2. Add 3 times the volume of 75% ethanol solution to the residue, stir and continue to extract in an ultrasonic water bath for 2h, the water bath temperature is 45 ° C, the ultrasonic power is 1000W, filter, and mix the filtrate with the extract obtained in step M1 to obtain a Bletilla striata extract; M3. The Bletilla striata extract is centrifuged, concentrated under reduced pressure, and then freeze-dried to obtain the Bletilla striata extract.

[0035] The white peony root extract is prepared by the following method: S1. 100g of white peony root was crushed, 200g of 0.2wt% aqueous hydrochloric acid solution was added and boiled at 80 ℃ for 1h, and 2 times the volume of water was added after acid boiling, and cooled to 45 ℃ to obtain white peony wet material; S2. Add 3g of cellulase and 1g of protease to the wet material of white peony root, and keep it at 45℃ for 1.5h; S3. The enzymatic hydrolysis product is centrifuged and filtered, and the filter residue is washed with an ethanol solution. The washing solution is combined with the filtrate to obtain a white peony extract, and the white peony extract is evaporated, concentrated, and dried to obtain a white peony extract.

[0036] This embodiment also provides a method for preparing the above-mentioned biopeptide antibacterial tablets, wherein the extract of Bletilla striata, the extract of Paeonia lactiflora, the biopeptide and chlorhexidine acetate are weighed and mixed evenly by mass percentage, and collagen, sodium hyaluronate and diguanosine tetraphosphate are mixed, and then mixed with the above-mentioned mixture, and oligosaccharide, cyclodextrin and starch are added and mixed, and finally magnesium stearate is added. After the above-mentioned materials are mixed evenly, they are compressed and granulated into tablets of 0.25 g / tablet.

[0037] Example 4 The present embodiment provides a biopeptide antibacterial tablet, whose components include 6% of bletilla striata extract, 6% of white peony root extract, 2% of biopeptide, 5% of collagen, 15% of sodium hyaluronate, 0.05% of diguanosine tetraphosphate, 5% of chlorhexidine acetate, 2% of isomaltooligosaccharide, 1% of magnesium stearate, 24% of cyclodextrin, and the balance is starch.

[0038] The bletilla striata extract is prepared by the following method: M1. Crush 100g of Bletilla striata tubers, add 300g of water, heat to 65℃ and extract for 8h, filter to obtain the extract and residue; M2. Add 80% ethanol solution twice the volume of the residue to the residue, stir and continue to extract in an ultrasonic water bath for 2h, the water bath temperature is 40 ° C, the ultrasonic power is 1000W, filter, and mix the filtrate with the extract obtained in step M1 to obtain a Bletilla striata extract; M3. The Bletilla striata extract is centrifuged, concentrated under reduced pressure, and then freeze-dried to obtain the Bletilla striata extract.

[0039] The white peony root extract is prepared by the following method: S1. 100g of white peony root was crushed, 200g of 0.5wt% citric acid aqueous solution was added and boiled at 80 ℃ for 2h, 3 times the volume of water was added after acid boiling, and cooled to 40 ℃ to obtain white peony wet material; S2. Add 4 g of cellulase and 2 g of protease to the wet material of white peony root, and keep it at 40°C for 2 h; S3. The enzymatic hydrolysis product is centrifuged and filtered, and the filter residue is washed with an ethanol solution. The washing solution is combined with the filtrate to obtain a white peony extract, and the white peony extract is evaporated, concentrated, and dried to obtain a white peony extract.

[0040] This embodiment also provides a method for preparing the above-mentioned biopeptide antibacterial tablets, wherein the extract of Bletilla striata, the extract of Paeonia lactiflora, the biopeptide and chlorhexidine acetate are weighed and mixed evenly by mass percentage, and collagen, sodium hyaluronate and diguanosine tetraphosphate are mixed, and then mixed with the above-mentioned mixture, and oligosaccharide, cyclodextrin and starch are added and mixed, and finally magnesium stearate is added. After the above-mentioned materials are mixed evenly, they are compressed and granulated into tablets of 0.25 g / tablet.

[0041] Comparative Example 1 This comparative example provides a bio-peptide antibacterial tablet, whose components include 6% of Bletilla striata extract, 6% of white peony root extract, 2% of bio-peptide, 5% of collagen, 15% of sodium hyaluronate, 0.05% of diguanosine tetraphosphate, 5% of chlorhexidine acetate, 2% of isomaltooligosaccharide, 1% of magnesium stearate, 24% of cyclodextrin, and the balance is starch.

[0042] The Bletilla striata extract was purchased from Shanghai Xinkai Pharmaceutical Technology Co., Ltd.

[0043] The white peony root extract is prepared by the following method: S1. 100g of white peony root was crushed, 200g of 0.2wt% aqueous hydrochloric acid solution was added and boiled at 80 ℃ for 1h, and 2 times the volume of water was added after acid boiling, and cooled to 45 ℃ to obtain white peony wet material; S2. Add 3g of cellulase and 1g of protease to the wet material of white peony root, and keep it at 45℃ for 1.5h; S3. The enzymatic hydrolysis product is centrifuged and filtered, and the filter residue is washed with an ethanol solution. The washing solution is combined with the filtrate to obtain a white peony extract, and the white peony extract is evaporated, concentrated, and dried to obtain a white peony extract.

[0044] The biopeptide antibacterial tablets of this comparative example are prepared by the following method: according to the mass percentage, the extract of Bletilla striata, the extract of Paeonia lactiflora, the biopeptide and the chlorhexidine acetate are weighed and mixed evenly, and collagen, sodium hyaluronate and diguanosine tetraphosphate are mixed, and then mixed with the above mixture, and oligosaccharide, cyclodextrin and starch are added and mixed, and finally magnesium stearate is added. After the above materials are mixed evenly, they are compressed and granulated into tablets of 0.25g / tablet.

[0045] Comparative Example 2 This comparative example provides a bio-peptide antibacterial tablet, whose components include 6% of Bletilla striata extract, 6% of white peony root extract, 2% of bio-peptide, 5% of collagen, 15% of sodium hyaluronate, 0.05% of diguanosine tetraphosphate, 5% of chlorhexidine acetate, 2% of isomaltooligosaccharide, 1% of magnesium stearate, 24% of cyclodextrin, and the balance is starch.

[0046] The bletilla striata extract is prepared by the following method: M1. Crush 100g of Bletilla striata tubers, add 300g of water, heat to 65℃ and extract for 8h, filter to obtain the extract and residue; M2. Add 80% ethanol solution twice the volume of the residue to the residue, stir and continue to extract in an ultrasonic water bath for 2h, the water bath temperature is 40 ° C, the ultrasonic power is 1000W, filter, and mix the filtrate with the extract obtained in step M1 to obtain a Bletilla striata extract; M3. The Bletilla striata extract is centrifuged, concentrated under reduced pressure, and then freeze-dried to obtain the Bletilla striata extract.

[0047] The white peony root extract was purchased from Shanghai Xinkai Pharmaceutical Technology Co., Ltd.

[0048] The biopeptide antibacterial tablets of this comparative example are prepared by the following method: according to the mass percentage, the extract of Bletilla striata, the extract of Paeonia lactiflora, the biopeptide and the chlorhexidine acetate are weighed and mixed evenly, and collagen, sodium hyaluronate and diguanosine tetraphosphate are mixed, and then mixed with the above mixture, and oligosaccharide, cyclodextrin and starch are added and mixed, and finally magnesium stearate is added. After the above materials are mixed evenly, they are compressed and granulated into tablets of 0.25g / tablet.

[0049] Comparative Example 3 This comparative example provides a bio-peptide antibacterial tablet, whose components include 6% of Bletilla striata extract, 6% of white peony root extract, 2% of bio-peptide, 5% of collagen, 15% of sodium hyaluronate, 0.05% of diguanosine tetraphosphate, 5% of chlorhexidine acetate, 1% of magnesium stearate, 24% of cyclodextrin, and the balance of starch.

[0050] The bletilla striata extract is prepared by the following method: M1. Crush 100g of Bletilla striata tubers, add 300g of water, heat to 65℃ and extract for 8h, filter to obtain the extract and residue; M2. Add 80% ethanol solution twice the volume of the residue to the residue, stir and continue to extract in an ultrasonic water bath for 2h, the water bath temperature is 40 ° C, the ultrasonic power is 1000W, filter, and mix the filtrate with the extract obtained in step M1 to obtain a Bletilla striata extract; M3. The Bletilla striata extract is centrifuged, concentrated under reduced pressure, and then freeze-dried to obtain the Bletilla striata extract.

[0051] The white peony root extract is prepared by the following method: S1. 100g of white peony root was crushed, 200g of 0.2wt% aqueous hydrochloric acid solution was added and boiled for 1h, and 2 times the volume of water was added after acid boiling, and cooled to 45 ℃ to obtain white peony wet material; S2. Add 3g of cellulase and 1g of protease to the wet material of white peony root, and keep it at 45℃ for 1.5h; S3. The enzymatic hydrolysis product is centrifuged and filtered, and the filter residue is washed with an ethanol solution. The washing solution is combined with the filtrate to obtain a white peony extract, and the white peony extract is evaporated, concentrated, and dried to obtain a white peony extract.

[0052] The biopeptide antibacterial tablets of this comparative example are prepared by the following method: according to the mass percentage, the extract of Bletilla striata, the extract of Paeonia lactiflora, the biopeptide and the chlorhexidine acetate are weighed and mixed evenly, and collagen, sodium hyaluronate and diguanosine tetraphosphate are mixed, and then mixed with the above mixture, cyclodextrin and starch are added and mixed, and finally magnesium stearate is added. After the above materials are mixed evenly, they are compressed and granulated into tablets of 0.25 g / tablet.

[0053] Comparative Example 4 This comparative example provides a bio-peptide antibacterial tablet, whose components include 6% of Bletilla striata extract, 6% of white peony root extract, 2% of bio-peptide, 5% of collagen, 15% of sodium hyaluronate, 0.05% of diguanosine tetraphosphate, 5% of chlorhexidine acetate, 2% of isomaltooligosaccharide, 1% of magnesium stearate, 24% of cyclodextrin, and the balance is starch.

[0054] The bletilla striata extract is prepared by the following method: M1. Crush 100g of Bletilla tubers, add 300g of 80% ethanol solution and heat to 40°C, ultrasonically extract for 8h at an ultrasonic power of 1000W, and filter to obtain the Bletilla extract and residue; M2. The Bletilla striata extract is centrifuged, concentrated under reduced pressure, and then freeze-dried to obtain the Bletilla striata extract.

[0055] The white peony root extract is prepared by the following method: S1. 100g of white peony root was crushed, 200g of 0.2wt% aqueous hydrochloric acid solution was added and boiled at 80 ℃ for 1h, and 2 times the volume of water was added after acid boiling, and cooled to 45 ℃ to obtain white peony wet material; S2. Add 3g of cellulase and 1g of protease to the wet material of white peony root, and keep it at 45℃ for 1.5h; S3. The enzymatic hydrolysis product is centrifuged and filtered, and the filter residue is washed with an ethanol solution. The washing solution is combined with the filtrate to obtain a white peony extract, and the white peony extract is evaporated, concentrated, and dried to obtain a white peony extract.

[0056] The biopeptide antibacterial tablets of this comparative example are prepared by the following method: according to the mass percentage, the extract of Bletilla striata, the extract of Paeonia lactiflora, the biopeptide and the chlorhexidine acetate are weighed and mixed evenly, and collagen, sodium hyaluronate and diguanosine tetraphosphate are mixed, and then mixed with the above mixture, and oligosaccharide, cyclodextrin and starch are added and mixed, and finally magnesium stearate is added. After the above materials are mixed evenly, they are compressed and granulated into tablets of 0.25g / tablet.

[0057] Comparative Example 5 This comparative example provides a bio-peptide antibacterial tablet, whose components include 6% of Bletilla striata extract, 6% of white peony root extract, 2% of bio-peptide, 5% of collagen, 15% of sodium hyaluronate, 0.05% of diguanosine tetraphosphate, 5% of chlorhexidine acetate, 2% of isomaltooligosaccharide, 1% of magnesium stearate, 24% of cyclodextrin, and the balance is starch.

[0058] The bletilla striata extract is prepared by the following method: M1. Crush 100g of Bletilla striata tubers, add 300g of water, heat to 65℃ and extract for 8h, filter to obtain the extract and residue; M2. Add 80% ethanol solution twice the volume of the residue to the residue, stir and continue to extract in an ultrasonic water bath for 2h, the water bath temperature is 40 ° C, the ultrasonic power is 1000W, filter, and mix the filtrate with the extract obtained in step M1 to obtain a Bletilla striata extract; M3. The Bletilla striata extract is centrifuged, concentrated under reduced pressure, and then freeze-dried to obtain the Bletilla striata extract.

[0059] The white peony root extract is prepared by the following method: S1. 100g of white peony root was crushed, 300g of 0.1wt% aqueous hydrochloric acid solution was added, 3g of cellulase and 1g of protease were added, and the mixture was boiled at 65°C for 6h, and one volume of water was added to the boiled mixture, and stirred and allowed to stand for 1h to obtain an enzymatic product; S2. The enzymatic hydrolysis product is centrifuged and filtered, and the filter residue is washed with an ethanol solution. The washing solution is combined with the filtrate to obtain a white peony extract, and the white peony extract is evaporated, concentrated, and dried to obtain a white peony extract.

[0060] The biopeptide antibacterial tablets of this comparative example are prepared by the following method: according to the mass percentage, the extract of Bletilla striata, the extract of Paeonia lactiflora, the biopeptide and the chlorhexidine acetate are weighed and mixed evenly, and collagen, sodium hyaluronate and diguanosine tetraphosphate are mixed, and then mixed with the above mixture, and oligosaccharide, cyclodextrin and starch are added and mixed, and finally magnesium stearate is added. After the above materials are mixed evenly, they are compressed and granulated into tablets of 0.25g / tablet.

[0061] Performance Testing 1. Solubility test Take one piece of each of the biopeptide antibacterial tablets prepared in Examples 1-4 and Comparative Examples 1-5, put them into 100 ml of warm water (35°C), stir, and observe until there is no obvious solid precipitation at the bottom of the solution. The dissolution time is recorded. The results are shown in Table 1.

[0062] Table 1

[0063] 2. Antibacterial performance test According to the test method of 5.1.1 in WS / T 650-2019 standard, the antibacterial performance of the biopeptide antibacterial tablets prepared in Examples 1-4 and Comparative Examples 1-5 was tested, wherein the biopeptide antibacterial tablets were dissolved in 100 ml of water to prepare sample solutions for antibacterial testing. The results are recorded in Table 2.

[0064] Table 2

[0065] It can be seen from the above performance test results that the bio-peptide antibacterial tablets of Examples 1-4 can dissolve faster, are easier to use, and shorten the processing time; and have excellent antibacterial effects on Escherichia coli, Staphylococcus aureus and Candida albicans, with an antibacterial rate of more than 95%. In particular, the comprehensive performance of Example 2 is the most outstanding, mainly because under the specific ratio of Bletilla striata extract and White Peony Root Extract in Example 2, a certain synergistic effect is exerted, wherein the Bletilla striata extract and White Peony Root Extract prepared under specific conditions contain higher effective active substances.

[0066] The comparative examples used commercially available Bletilla striata extract and White Peony Root extract, or were prepared by extraction methods different from those of the present invention, which affected the content of effective active substances, thereby resulting in poor antibacterial performance of the final bio-peptide antibacterial tablet product.

[0067] The above is a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A biopeptide antibacterial tablet, characterized in that: The components include Bletilla Striata extract, White Peony (Cynanchum Otophyllum) extract, biological peptides, collagen, sodium hyaluronate, diguanosine tetraphosphate, chlorhexidine acetate and auxiliary materials.

2. The biopeptide antibacterial tablet according to claim 1, characterized in that: The mass fractions of the components are 4-8% of bletilla striata extract, 6-8% of white peony root extract, 2-3% of biological peptides, 5-10% of collagen, 12-18% of sodium hyaluronate, 0.02-0.2% of diguanosine tetraphosphate, and 4-8% of chlorhexidine acetate.

3. The biopeptide antibacterial tablet according to claim 1, characterized in that: Calculated by mass percentage of the bio-peptide antibacterial tablets, the auxiliary materials include 2-5% isomaltooligosaccharide, 1-2% magnesium stearate, 20-30% cyclodextrin, and the balance is starch.

4. The biopeptide antibacterial tablet according to claim 1, characterized in that: The bletilla striata extract is prepared by the following method: M1. Crush the tubers of Bletilla striata, extract with hot water for 5-10 hours, and filter to obtain the extract and residue; M2. Add 70-95% ethanol solution to the residue, stir and continue to extract in an ultrasonic water bath for 1-3 hours, filter with suction, and mix the filtrate with the extract obtained in step M1 to obtain a Bletilla striata extract; M3. The Bletilla striata extract is centrifuged, concentrated under reduced pressure, and then freeze-dried to obtain the Bletilla striata extract.

5. The biopeptide antibacterial tablet according to claim 4, characterized in that: The temperature of hot water extraction in the M1 step is 60-75° C., and the mass ratio of water to the crushed Bletilla striata tuber is 2-6:

1.

6. The biopeptide antibacterial tablet according to claim 4, characterized in that: The water bath temperature in the M2 step is 35-45° C., and the volume ratio of the ethanol solution to the filter residue is 1.5-3:

1.

7. The biopeptide antibacterial tablet according to claim 1, characterized in that: The white peony root extract is prepared by the following method: S1. Crush the white peony root, add acid and cook for 1-2h, add water, and cool to below 45°C to obtain white peony wet material; S2. Add cellulase and protease to the white peony wet material, and keep warm for enzymolysis for 1-2 hours; S3. The enzymatic hydrolysis product is centrifuged and filtered, and the filter residue is washed with an ethanol solution. The washing solution is combined with the filtrate to obtain a white peony extract, and the white peony extract is evaporated, concentrated, and dried to obtain a white peony extract.

8. The biopeptide antibacterial tablet according to claim 7, characterized in that: The acid in step S1 is hydrochloric acid or citric acid solution, with a concentration of 0.1-1 wt %, and a mass ratio of acid to white peony root of 1.5-2:

1.

9. The biopeptide antibacterial tablet according to claim 7, characterized in that: The added amount of the cellulase is 2-5% of the weight of the white peony root, and the added amount of the protease is 1-3% of the weight of the white peony root.

10. A method for preparing the biopeptide antibacterial tablet according to any one of claims 1 to 9, characterized in that: The method comprises the following steps: uniformly mixing bletilla striata extract, white peony root extract, biological peptide, collagen, sodium hyaluronate, diguanosine tetraphosphate, chlorhexidine acetate and auxiliary materials, compressing and granulating, and tableting.

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