Multifunctional molecular biology pipettor and use method thereof

Abstract

The invention discloses a multifunctional molecular biology pipettor and a using method thereof, and belongs to the technical field of pipettes, the pipettor comprises a pipettor body and a gun head arranged on the pipettor body in a sleeving mode, a negative pressure module used for driving the gun head to suck and discharge liquid is arranged in the pipettor body, the negative pressure module comprises a negative pressure device and a liquid sucking and discharging unit, and the negative pressure device is connected with the pipettor body. The negative pressure device is used for providing driving force for liquid suction and liquid discharge, the liquid suction and liquid discharge unit comprises a liquid suction and liquid discharge pipe arranged at the front end of the pipettor body, and an optical sensor and / or an ultrasonic sensor are / is arranged in the liquid suction and liquid discharge pipe and used for monitoring the liquid volume in real time; a fluid resistance sensor is further arranged in the liquid suction and drainage pipe and used for detecting the flowing resistance of liquid in real time. The negative pressure module further comprises an automatic adjusting unit, starting and stopping of the negative pressure device are controlled according to signals of the optical sensor or the ultrasonic sensor, and the liquid suction and drainage speed and pressure are automatically adjusted according to signals of the fluid resistance sensor. The design can ensure the accuracy and reliability of the pipetting operation.

Description

Technical Field

[0001] The present invention relates to the technical field of pipettes, and more particularly to a multifunctional molecular biology pipette and a method for using the same. Background Art

[0002] A pipette, also known as a pipette gun, is a measuring tool that transfers liquid reagents from an original container to another container within a certain range. It is widely used in biology, chemistry and other fields. Commonly used pipettes in the prior art include manual pipettes and electric pipettes. Manual pipettes generally control the volume of the pipette by sliding the tube body or compressing the piston. When multiple steps are required to measure the target liquid in real time, the pipette volume needs to be repeatedly set and measured repeatedly. The operation is time-consuming and laborious, and the target liquid may not be completely absorbed in the end. The electric pipette can be driven by a motor to make the piston between the suction tip and the leather tip move up and down to achieve quantitative extraction of liquid, but the stepper motor has the risk of losing steps or losing steps during use, which will lead to inaccurate measurements. In addition, since the electric pipette absorbs liquid at a constant rate, it is impossible to adjust the adaptive rate for different liquid samples.

[0003] Patent document CN117753493A discloses a manual pipette with a liquid measuring function. By setting a first limit block in the gun body and setting the maximum stroke of the I-shaped piston moving downward, it is convenient to set the zero scale value position of the float, so that the operator can measure and display the volume of the liquid absorbed in real time when absorbing the target liquid of unknown volume in the container. At the same time, it also has the function of quantitatively absorbing a certain volume of liquid possessed by conventional pipettes, and can adjust the liquid absorption speed in real time according to experimental needs to make up for the defect of constant rate of electric pipettes. However, traditional molecular biology pipettes have many challenges in terms of operation accuracy, data management and user training. The main technical problems include: it is difficult to achieve high-precision liquid volume measurement and viscosity detection, and errors are prone to occur in operation; sample identification and tracking rely on manual records, and there is a risk of incomplete data and misoperation; user operation skills are not easy to quantify and improve, and it is difficult to ensure the consistency and standardization of operations. The above problems affect the reliability and repeatability of experimental results, and increase the complexity and workload of experimental operations.

[0004] Therefore, it is necessary to provide a multifunctional molecular biology pipette and a method of using the same to overcome the shortcomings of existing pipettes, ensure the accuracy and reliability of pipetting operations, provide instant feedback and optimization suggestions, and improve the standardization and consistency of user operations. Summary of the invention

[0005] 1. Technical issues to be solved

[0006] The purpose of the present application is to provide a multifunctional molecular biology pipette and a method for using the same, which solves the technical problem raised in the above-mentioned background technology that the existing pipettes are difficult to achieve high-precision liquid volume measurement and constant liquid aspiration rate. By integrating multiple sensors and advanced algorithms, high-precision liquid volume measurement and viscosity detection are achieved, and the liquid extraction parameters can be adaptively adjusted according to the liquid viscosity to ensure the accuracy and reliability of pipetting operations under different sample properties. By real-time monitoring and analysis of user operation behavior, instant feedback and optimization suggestions are provided, which significantly improves the standardization and consistency of user operations.

[0007] 2. Technical solution

[0008] Based on the above purpose, the present application provides a multifunctional molecular biology pipette, including a pipette body and a gun tip mounted on the pipette body, wherein the pipette body is provided with a negative pressure module for driving the gun tip to aspirate and discharge liquid. The negative pressure module includes a negative pressure device and a liquid aspiration and discharge unit, wherein the negative pressure device is used to provide a driving force for aspiration and discharge, and the liquid aspiration and discharge unit includes a liquid aspiration and discharge tube disposed at the front end of the pipette body, wherein an optical sensor and / or an ultrasonic sensor is disposed in the liquid aspiration and discharge tube for real-time monitoring of the liquid volume, and a fluid resistance sensor is also disposed in the liquid aspiration and discharge tube for real-time detection of the flow resistance of the liquid. The negative pressure module also includes an automatic adjustment unit, which controls the start and stop of the negative pressure device according to the optical sensor / or ultrasonic sensor signal, and automatically adjusts the speed and pressure of liquid aspiration and discharge according to the fluid resistance sensor signal.

[0009] Furthermore, an adjustment matching model is built in the automatic adjustment unit. The model input is the fluid resistance and fluid volume signal detected by the fluid resistance sensor, which automatically adjusts the operating parameters of the negative pressure device, and the output is the corresponding suction and discharge speed and pressure.

[0010] Furthermore, the multifunctional molecular biology pipette also includes a sample identification module, which includes a spectral sensor and a microscopic camera installed on the pipette body, for scanning and analyzing the color, density and morphological characteristics of the sample. The sample identification module is also equipped with a QR code scanner or an RFID reader for identifying and tracking sample information.

[0011] Furthermore, the sample identification module also includes an identification and tracking unit. The identification and tracking unit verifies the identity of the sample based on the multi-dimensional information of the sample collected by the spectral sensor and the microscope camera, and automatically records the sample information. Through QR code scanning or RFID reading, it realizes automatic tracking and full-process recording of the sample.

[0012] In order to realize real-time monitoring and correction of user operation defects, the multifunctional molecular biology pipette also includes a user behavior monitoring module. The user behavior monitoring module includes an accelerometer and a gyroscope sensor, which is built into the handle of the pipette body and is used to monitor the user's operation posture, strength and angle in real time.

[0013] Furthermore, the user behavior monitoring module also includes a user behavior analysis unit and a real-time feedback unit. The user behavior analysis unit performs real-time analysis on the time series data of user operations based on the LSTM model and outputs regression prediction results of the operation behavior. The real-time feedback unit combines feature extraction and real-time feedback mechanism to generate corresponding operation feedback and optimization suggestions.

[0014] In addition, the multifunctional molecular biology pipette also includes a data processing and communication module, which is used to process the data of each sensor and upload the data to the cloud through wireless communication. The smart terminal provides identification and tracking information chain and real-time feedback based on the monitoring data.

[0015] The present application also provides a method for using a multifunctional molecular biology pipette, comprising the following steps:

[0016] Start calibration: Start the pipette body, perform self-check and calibration based on optical and ultrasonic sensors to ensure that the negative pressure module is in the best condition;

[0017] Sample identification: Align the sample identification module of the pipette with the sample container, and the spectral sensor and microscope camera scan the color, density and morphological characteristics of the sample, identify its characteristics and obtain sample information through the QR code or RFID tag;

[0018] Aspiration operation: Set the aspiration volume, optical sensors and / or ultrasonic sensors monitor the aspiration process in real time, fluid resistance sensors detect liquid viscosity, and automatically adjust the aspiration speed and pressure;

[0019] Discharging operation: After the aspiration is completed, the pipette is moved to the target container position to discharge the liquid. The discharging process is also monitored and adjusted by optical sensors and / or ultrasonic sensors and fluid resistance sensors to ensure accurate discharging;

[0020] Data recording and tracking: The pipette automatically records the data of each aspiration and discharge, including volume, viscosity, and sample characteristics. The data is uploaded to the cloud for storage via the wireless communication module;

[0021] User behavior feedback: Use accelerometers and gyroscope sensors to monitor time series data including but not limited to operation posture and force in real time, perform real-time analysis on the time series data of user operations, and generate corresponding operation feedback and optimization suggestions.

[0022] 3. Beneficial effects

[0023] The multifunctional molecular biology pipette and the method of using the same in the present application have at least the following technical effects or advantages:

[0024] The multifunctional intelligent molecular biology pipette integrates multiple sensors and advanced algorithms to achieve high-precision liquid volume measurement and viscosity detection, ensuring the accuracy of each pipetting operation; it automatically identifies and tracks sample information through spectral analysis and microscopic imaging technology, improving the integrity and traceability of data management; it monitors user operation behavior in real time through accelerometers and gyroscope sensors, analyzes operation modes with LSTM algorithms, provides instant feedback and optimization suggestions, and significantly improves the standardization and consistency of user operations. This can significantly improve the efficiency, accuracy and data reliability of experimental operations, reduce human errors, and simplify experimental processes. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 It is a schematic diagram of the structural framework of the multifunctional molecular biology pipette of the present application;

[0026] Figure 2 It is a schematic diagram of the structure of the sample identification module in the multifunctional molecular biology pipette of the present application;

[0027] Figure 3 It is a structural schematic diagram of a user behavior monitoring module in the multifunctional molecular biology pipette of the present application;

[0028] Figure 4 It is a process framework diagram of the method for using the multifunctional molecular biology pipette of the present application. DETAILED DESCRIPTION

[0029] In order to enable those skilled in the art to better understand the technical solutions in this specification, the technical solutions in the embodiments of this specification will be clearly and completely described below in conjunction with the drawings in the embodiments of this specification. Obviously, the described embodiments are only part of the embodiments of this application, not all of the embodiments.

[0030] Example 1

[0031] See also Figure 1The embodiment of the present invention provides a multifunctional molecular biology pipette, including a pipette body and a gun tip mounted on the pipette body, wherein the pipette body is provided with a negative pressure module for driving the gun tip to aspirate and discharge liquid. The negative pressure module includes a negative pressure device and a liquid aspiration and discharge unit, wherein the negative pressure device is used to provide a driving force for aspiration and discharge, and the liquid aspiration and discharge unit includes a liquid aspiration and discharge tube disposed at the front end of the pipette body, wherein an optical sensor and / or an ultrasonic sensor is disposed in the liquid aspiration and discharge tube for real-time monitoring of the liquid volume, and a fluid resistance sensor is also disposed in the liquid aspiration and discharge tube for real-time detection of the flow resistance of the liquid. The negative pressure module also includes an automatic adjustment unit, which controls the start and stop of the negative pressure device according to the optical sensor / or ultrasonic sensor signal, and automatically adjusts the speed and pressure of liquid aspiration and discharge according to the fluid resistance sensor signal.

[0032] In this embodiment, an optical sensor and an ultrasonic sensor are provided in the pipette and discharge tube. The optical sensor detects the reflection and refraction changes on the liquid surface by emitting and receiving light beams. Whenever the liquid enters or exits the pipette and discharge tube, the reflection and refraction angles of the light beams will change. The optical sensor captures these changes in real time and transmits the data to the automatic adjustment unit built into the pipette body. The automatic adjustment unit calculates the volume of the liquid based on the reflection and refraction data. The ultrasonic sensor detects the position and movement speed of the liquid by emitting ultrasonic waves and receiving echo signals, and accurately measures the volume of the liquid by the propagation time and intensity changes of the ultrasonic signal. This embodiment uses a combination of optical sensors and ultrasonic sensors to ensure high precision and real-time performance of liquid volume measurement.

[0033] The fluid resistance sensor is embedded in the suction and discharge tube. When the liquid is sucked into the suction tube, the fluid resistance value detected by the sensor is related to the viscosity of the liquid. The fluid resistance sensor transmits the resistance data to the automatic adjustment unit, and the automatic adjustment unit calculates the viscosity value of the liquid in real time. A regulation matching model is built in the automatic adjustment unit based on a deep neural network. The regulation matching model is trained based on data consisting of the detected viscosity value, volume value, and the speed and pressure value of suction and discharge. The number of neurons in the input layer of the model neural network is 2, representing the liquid viscosity value and liquid volume value respectively, and the number of neurons in the hidden layer is two. The hidden layer uses the ReLU function as the activation function. The formula of the ReLU function is f(x)=max(0,x). The output layer is 2 neurons, representing the speed and pressure of liquid suction and discharge, respectively. The activation function uses a linear function, and the loss function is a smooth L1 loss L1=(1 / n)∑|yy′|, where y is the true value, y′ is the predicted value, and n is the number of samples. The optimizer uses a stochastic gradient descent optimizer with an initial learning rate of 0.001, which is decayed according to the change of the loss function, and the gradient is calculated using back propagation. The detected viscosity value and volume value are input into the adjustment matching model, and the operating parameters of the negative pressure device are automatically adjusted, and the output is the corresponding speed and pressure of liquid suction and discharge. For example, when a high-viscosity liquid is detected, the system will slow down the liquid suction speed and increase the suction pressure to ensure that the liquid can be sucked in smoothly; when discharging, the system will also adjust the liquid discharge speed and pressure accordingly to ensure that the high-viscosity liquid can be discharged smoothly. This real-time adjustment mechanism greatly improves the accuracy and adaptability of the pipetting operation.

[0034] By combining optical sensors and ultrasonic sensors, high-precision real-time measurement of liquid volume is achieved, and the fluid resistance sensor is embedded in the pipeline. By real-time detection of viscosity and automatic adjustment of operating parameters, the intelligence level and operational adaptability of the system are improved. The combination of the two greatly improves the performance and application range of the pipette.

[0035] For further information, see Figure 2 The multifunctional molecular biology pipette also includes a sample identification module, which includes a spectral sensor and a microscopic camera installed on the pipette body, which are used to scan and analyze the color, density and morphological characteristics of the sample. The sample identification module is also equipped with a QR code scanner or RFID reader for identifying and tracking sample information. The sample identification module also includes an identification and tracking unit, which is based on the multi-dimensional information of the sample collected by the spectral sensor and the microscopic camera, double-identifies and verifies the identity of the sample, and automatically records the sample information. Through QR code scanning or RFID reading, the automatic tracking and full-process recording of the sample can be realized.

[0036] In this embodiment, the sample identification module integrates a spectral sensor and a microscopic camera. The spectral sensor analyzes the spectral characteristics of the sample by emitting a beam of a specific wavelength and receiving reflected light, thereby accurately determining the color and density of the sample. The microscopic camera performs high-resolution imaging of the sample to capture the morphological characteristics of the sample, such as particle size, shape, and surface structure. When the sample is placed in front of the sample identification module, the spectral sensor and the microscopic camera work synchronously to obtain the spectral data and microscopic image of the sample. The identification and tracking unit processes and analyzes the spectral data and microscopic image of the sample in real time, such as comparing the spectral data with the known sample characteristics in the database to confirm the type and composition of the sample, and further verifies the identity of the sample through the morphological characteristics provided by the microscopic camera to ensure the accuracy of the identification. After the identification is completed, the identification and tracking unit automatically records the sample information, including color, density, morphological characteristics, and processing conditions, and realizes automatic tracking and full-process recording of the sample through QR code scanning or RFID reading. Through this multi-dimensional data analysis and comparison, the sample identification module realizes high-precision automatic identification and tracking of the sample, ensuring the integrity and traceability of the experimental data.

[0037] By combining spectral analysis and microscopic imaging technology, multi-dimensional identification and tracking of samples can be achieved. The spectral sensor provides accurate analysis of the sample's color and density, and the microscopic camera captures the sample's morphological characteristics. Double verification ensures the accuracy of the identification results, and automatically records and tracks sample information, improving the intelligence level of the experimental process and the reliability of data management.

[0038] See also Figure 3 In order to monitor and correct user operation defects in real time, the multifunctional molecular biology pipette also includes a user behavior monitoring module. The user behavior monitoring module includes an accelerometer and a gyroscope sensor, which are built into the handle of the pipette body and are used to monitor the posture, strength and angle of the user operation in real time. The user behavior monitoring module also includes a user behavior analysis unit and a real-time feedback unit. The user behavior analysis unit performs real-time analysis on the time series data of user operations based on the LSTM model and outputs the regression prediction results of the operation behavior. The real-time feedback unit combines feature extraction and real-time feedback mechanism to generate corresponding operation feedback and optimization suggestions.

[0039] In this embodiment, the accelerometer and gyroscope sensor of the user behavior monitoring module are installed on the handle of the pipette. The accelerometer can detect the acceleration change of the pipette in three axes (X, Y, Z), thereby measuring the strength and movement speed of the user's operation. The gyroscope sensor detects the angular velocity of the pipette on three rotation axes (pitch, roll, yaw), monitors the angle and posture changes of the user's operation in real time, and forms a comprehensive three-dimensional operation behavior map by collecting continuous acceleration and angular velocity data. The user behavior analysis unit processes and analyzes the above data in real time, evaluates the user's operation stability and accuracy, and then identifies the wrong or irregular operation posture and strength to help the user make timely adjustments.

[0040] In order to achieve real-time analysis of user operation patterns and behaviors, generate operation feedback and optimization suggestions, supervised learning and time series analysis algorithms in machine learning can be used, such as using LSTM to process and analyze user operation data to capture dynamic changes and patterns in the user operation process. The construction process of the LSTM model is as follows:

[0041] (1) Data collection and preprocessing

[0042] Data collection: The accelerometer and gyroscope sensors collect real-time data of user operations, including three-axis acceleration and three-axis angular velocity data.

[0043] Data preprocessing: De-noise, standardize and segment the collected data. Remove abnormal data points and standardize the data in each dimension to make its mean 0 and variance 1. Divide the continuous time series data into time windows of fixed length (such as data per second as a time window).

[0044] (2) Feature extraction

[0045] Time domain features: Extract basic features from each time window, such as mean, variance, maximum, minimum, peak, etc.

[0046] Frequency domain features: Perform fast Fourier transform (FFT) on the data in each time window to extract frequency domain features, such as main frequency, spectrum energy, etc.

[0047] Custom features: Extract specific operation behavior features according to specific application requirements, such as hand shake frequency, rotation angle change rate, etc.

[0048] (3) LSTM model construction

[0049] Model input: The preprocessed time series data is used as the input of the LSTM network. The dimension of the input data is (time window length, number of features).

[0050] Model structure: The input layer receives preprocessed time series data. The LSTM layer contains several LSTM units, which are used to process time series data and capture the dynamic changes of operation behaviors. Multiple layers of LSTM can be stacked to increase the depth and expression ability of the model. The fully connected layer connects the output of the LSTM layer for further feature extraction and processing. The output layer outputs the operation mode classification results or regression prediction results according to the specific task.

[0051] The user operation data collected in real time is input into the trained LSTM model through preprocessing and feature extraction steps. The LSTM model outputs the regression prediction results of the operation behavior based on the real-time input data.

[0052] Based on the analysis results, the system provides instant operational feedback and optimization suggestions. If the system detects that the user's operating posture is unstable or the force is uneven, it will issue a warning and provide corrective suggestions, such as "The operating force is too large, please reduce the force", "The posture is unstable, please adjust the wrist angle", etc. The above warning content can be displayed through the high-resolution touch screen set on the outer surface of the pipette body. Users can gradually improve their operating skills based on these instant feedback and suggestions, and improve the accuracy and efficiency of pipetting operations.

[0053] By integrating accelerometers and gyroscope sensors, comprehensive monitoring and real-time feedback of user operation behaviors are achieved. The data of accelerometers and gyroscope sensors are used in combination to provide a detailed three-dimensional operation behavior map to ensure accurate monitoring of operation posture, force and angle. By using the LSTM model to perform real-time analysis on the time series data of user operations, the dynamic changes and patterns in the user operation process can be accurately captured. Combined with feature extraction and real-time feedback mechanisms, the standardization and accuracy of user operations can be improved. This method is not only applicable to pipetting operations, but can also be extended to other operation scenarios that require real-time monitoring and feedback.

[0054] In addition, the multifunctional molecular biology pipette also includes a data processing and communication module, which is used to process the data of each sensor and upload the data to the cloud through wireless communication. The smart terminal provides identification and tracking information chain and real-time feedback based on the monitoring data. The real-time data collected by various sensors is transmitted to terminals such as smart devices or computers through the built-in wireless module of the pipette, such as Bluetooth or Wi-Fi. The data transmission process uses encryption technology to ensure the security and integrity of the data.

[0055] Example 2

[0056] The following is a description of the method for using the multifunctional molecular biology pipette provided by the present invention, which can be used in conjunction with the multifunctional molecular biology pipette described above. Figure 4 As shown, the embodiment of the present invention discloses a method for using a multifunctional molecular biology pipette, comprising the following steps:

[0057] Start calibration: Start the pipette body, perform self-check and calibration based on optical and ultrasonic sensors to ensure that the negative pressure module is in the best condition;

[0058] Sample identification: Align the sample identification module of the pipette with the sample container, and the spectral sensor and microscope camera scan the color, density and morphological characteristics of the sample, identify its characteristics and obtain sample information through the QR code or RFID tag;

[0059] Aspiration operation: Set the aspiration volume, optical sensors and / or ultrasonic sensors monitor the aspiration process in real time, fluid resistance sensors detect liquid viscosity, and automatically adjust the aspiration speed and pressure;

[0060] Discharging operation: After the aspiration is completed, the pipette is moved to the target container position to discharge the liquid. The discharging process is also monitored and adjusted by optical sensors and / or ultrasonic sensors and fluid resistance sensors to ensure accurate discharging;

[0061] Data recording and tracking: The pipette automatically records the data of each aspiration and discharge, including volume, viscosity, and sample characteristics. The data is uploaded to the cloud for storage via the wireless communication module;

[0062] User behavior feedback: Use accelerometers and gyroscope sensors to monitor time series data including but not limited to operation posture and force in real time, perform real-time analysis on the time series data of user operations, and generate corresponding operation feedback and optimization suggestions.

[0063] It should be noted that the system provided in the above embodiment only uses the division of the above functional modules as an example to implement its functions. In actual applications, the above functions can be assigned to different functional modules as needed, that is, the internal structure of the device can be divided into different functional modules to complete all or part of the functions described above. In addition, the system and method embodiments provided in the above embodiment belong to the same concept, and the specific implementation process is detailed in the method embodiment, which will not be repeated here.

[0064] In the above embodiments, the description of each embodiment has its own emphasis. For parts that are not described in detail in a certain embodiment, reference can be made to the relevant descriptions of other embodiments.

Claims

1. A multifunctional molecular biology pipette, comprising a pipette body and a gun tip sleeved on the pipette body, wherein the pipette body has a negative pressure module for driving the gun tip to absorb and discharge liquid. Features: The negative pressure module includes a negative pressure device and a liquid suction and discharge unit. The negative pressure device is used to provide a driving force for suction and discharge. The liquid suction and discharge unit includes a liquid suction and discharge tube arranged at the front end of the pipette body. An optical sensor and / or an ultrasonic sensor is arranged in the liquid suction and discharge tube for real-time monitoring of the liquid volume. A fluid resistance sensor is also provided in the liquid suction and discharge pipe to detect the flow resistance of the liquid in real time; The negative pressure module also includes an automatic adjustment unit, which controls the start and stop of the negative pressure device according to the optical sensor / or ultrasonic sensor signal, and automatically adjusts the speed and pressure of liquid suction and discharge according to the fluid resistance sensor signal.

2. The multifunctional molecular biology pipette according to claim 1, Features: An adjustment matching model is built in the automatic adjustment unit. The model input is the fluid resistance and fluid volume signal detected by the fluid resistance sensor. The operating parameters of the negative pressure device are automatically adjusted, and the output is the corresponding liquid suction and discharge speed and pressure.

3. The multifunctional molecular biology pipette according to claim 1, Features: It also includes a sample identification module, which includes a spectral sensor and a microscope camera installed on the pipette body, and is used to scan and analyze the color, density and morphological characteristics of the sample.

4. The multifunctional molecular biology pipette according to claim 3, Features: The sample identification module is also equipped with a QR code scanner or RFID reader for identifying and tracking sample information.

5. The multifunctional molecular biology pipette according to claim 4, Features: The sample identification module also includes an identification and tracking unit. The identification and tracking unit verifies the identity of the sample based on the multi-dimensional information of the sample collected by the spectral sensor and the microscope camera, and automatically records the sample information. Through QR code scanning or RFID reading, it realizes automatic tracking and full-process recording of the sample.

6. The multifunctional molecular biology pipette according to claim 1, Features: It also includes a user behavior monitoring module, which includes an accelerometer and a gyroscope sensor, which is built into the handle of the pipette body and is used to monitor the user's operation posture, strength and angle in real time.

7. The multifunctional molecular biology pipette according to claim 6, Features: The user behavior monitoring module also includes a user behavior analysis unit and a real-time feedback unit. The user behavior analysis unit performs real-time analysis on the time series data of user operations based on the LSTM model and outputs the regression prediction results of the operation behavior. The real-time feedback unit combines feature extraction and real-time feedback mechanism to generate corresponding operation feedback and optimization suggestions.

8. The multifunctional molecular biology pipette according to any one of claims 1 to 7, Features: It also includes a data processing and communication module, which is used to process the data of each sensor and upload the data to the cloud through wireless communication. The smart terminal provides identification and tracking information chain and real-time feedback based on the monitoring data.

9. A method for using a multifunctional molecular biology pipette. It is characterized in that Based on the multifunctional molecular biology pipette according to claims 1-8, the method comprises the following steps: Start calibration: Start the pipette body, perform self-check and calibration based on optical and ultrasonic sensors to ensure that the negative pressure module is in the best condition; Sample identification: Align the sample identification module of the pipette with the sample container, and the spectral sensor and microscope camera scan the color, density and morphological characteristics of the sample, identify its characteristics and obtain sample information through the QR code or RFID tag; Aspiration operation: Set the aspiration volume, optical sensors and / or ultrasonic sensors monitor the aspiration process in real time, fluid resistance sensors detect liquid viscosity, and automatically adjust the aspiration speed and pressure; Dispensing operation: After aspiration is completed, the pipette is moved to the target container position to discharge the liquid. The discharging process is also monitored and adjusted by optical sensors and / or ultrasonic sensors and fluid resistance sensors to ensure accurate discharging.

10. The method for using the multifunctional molecular biology pipette according to claim 9, It is characterized in that The following steps are also included: Data recording and tracking: The pipette automatically records the data of each aspiration and discharge, including volume, viscosity, and sample characteristics. The data is uploaded to the cloud for storage via the wireless communication module; User behavior feedback: Use accelerometers and gyroscope sensors to monitor time series data including but not limited to operation posture and force in real time, perform real-time analysis on the time series data of user operations, and generate corresponding operation feedback and optimization suggestions.

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