Foliar fertilizer for mulberry leaves and preparation method thereof

Abstract

Belonging to the technical field of fertilizers, the invention discloses a mulberry leaf foliar fertilizer, which comprises the following components: mulberry leaf protein and titanium dioxide, the invention also discloses a preparation method of the mulberry leaf foliar fertilizer. According to the present invention, the mulberry leaf protein contains a variety of amino acids, can improve the nutritional value of the mulberry leaf, contains alkaloid and flavonoid compounds, has effects of antibacterial, antiviral and antioxidation, can avoid the invasion and the reproduction of plant diseases and insect pests, and can reduce the occurrence of the plant diseases and insect pests; meanwhile, the mulberry leaf protein can serve as a carrier of titanium dioxide to help the titanium dioxide to be more uniformly dispersed on the leaf surfaces of the mulberry leaves, the promoting effect of the titanium dioxide on photosynthesis of the leaf surfaces of the mulberry leaves is improved, the yield and quality of the mulberry leaves are improved, and meanwhile the mulberry leaf protein and the titanium dioxide cannot pollute the surrounding environment; and even the surrounding environment air can be purified, the surrounding environment soil can be restored, and the practicability of the mulberry leaf foliar fertilizer is improved.

Description

Technical Field

[0001] The invention belongs to the technical field of fertilizers, and in particular relates to a mulberry leaf foliar fertilizer and a preparation method thereof. Background Art

[0002] Mulberry leaves are the leaves of the economic crop mulberry tree. They are an important raw material for sericulture production, and their yield and quality are directly related to the quality of cocoons and raw silk.

[0003] Traditional soil fertilization methods are often restricted by factors such as climate and soil conditions, and it is difficult to meet the nutritional needs of mulberry trees throughout the year, resulting in the yield and quality of mulberry leaves being affected. In order to solve the above problems, foliar fertilization is used as a fast and effective supplement method, which can directly provide mulberry leaves with the required nutrients and improve the yield and quality of mulberry leaves.

[0004] Chinese Patent No. 00112224.X discloses a mulberry leaf fertilizer comprising: NaHSO3, KH2PO4, and vitamin C.

[0005] The above technology has the following problems: although the components of the above mulberry leaf fertilizer can improve the resistance of mulberry leaves and increase the yield and quality of mulberry leaves, it will affect the surrounding environment as it evaporates at high temperature or rain falls, and its practicality still needs to be improved.

[0006] In view of this, a mulberry leaf foliar fertilizer and a preparation method are designed to solve the above problems. Summary of the invention

[0007] In order to solve the problems raised in the above background technology, the present invention provides a mulberry leaf foliar fertilizer and a preparation method thereof, which has the characteristics of improving the practicality of the mulberry leaf foliar fertilizer.

[0008] Another object of the present invention is to provide a method for preparing mulberry leaf foliar fertilizer.

[0009] To achieve the above object, the present invention provides the following technical solution: a mulberry leaf foliar fertilizer, comprising the following components: mulberry leaf protein and titanium dioxide.

[0010] The method for preparing the mulberry leaf foliar fertilizer comprises the following steps: S1: Pick fresh mulberry leaves free of pests and diseases; S2: The harvested mulberry leaves are placed in a clean water pool for washing to remove dust, pesticide residues and other impurities on the surface; S3: placing the washed mulberry leaves in a dryer for drying to remove surface moisture; S4: placing the dried mulberry leaves in a grinder and grinding them to obtain mulberry leaf powder; S5: placing mulberry leaf powder and extraction solvent in an ultrasonic device according to a certain proportion, and performing ultrasonic treatment. The ultrasonic wave cavitation, stirring and breaking the cell wall of the mulberry leaf make the mulberry leaf protein fully released into the extraction solvent to obtain a mixed solution; S6: placing the mixed solution in a centrifuge for centrifugation to separate the supernatant and the mulberry leaf protein precipitate; S7: purifying the separated supernatant based on ion exchange resin to obtain mulberry leaf protein; S8: placing the titanium dioxide powder in a sieving machine for sieving to remove titanium dioxide powder with inconsistent particle sizes; S9: placing mulberry leaf protein and sieved titanium dioxide powder in a container according to a proportion, adding deionized water according to a proportion, stirring until the mixture is evenly mixed, and transporting the mixture to a filling machine through a pipeline; S10: The filling machine fills equal amounts into packaging bags or bottles and seals them.

[0011] Furthermore, in step S5, the extraction solvent is sodium hydroxide solution, and the liquid-to-solid ratio of the sodium hydroxide solution is 50-100:1.

[0012] Furthermore, in step S5, the adding ratio of mulberry leaf powder and extraction solvent is 1:20-25.

[0013] Furthermore, in step S5, during the ultrasonic process, acid or alkaline solution is added to the mulberry leaf powder and the extraction solvent according to the pH value of the extraction solvent to adjust the pH value of the extraction solvent so that the pH value is maintained at 7-9.

[0014] Furthermore, the specific steps of step S7 include: S71: slowly loading the supernatant onto the equilibrated ion exchange resin to allow the mulberry leaf protein to fully contact and bind to the medium; S72: pouring the buffer solution onto the ion exchange resin to elute the mulberry leaf protein and other impurities that are not bound to the medium on the ion exchange resin; S73: gradually increase the ion concentration of the buffer to gradually elute the mulberry leaf protein bound to the medium; S74: collecting the eluate containing mulberry leaf protein according to the elution curve and the properties of mulberry leaf protein; S75: The collected eluate containing mulberry leaf protein is subjected to ultrafiltration treatment to remove residual salts and other small molecular substances to obtain mulberry leaf protein.

[0015] Furthermore, in step S9, the addition ratio of mulberry leaf protein and titanium dioxide powder is 1:2-3, and the addition ratio of deionized water to the mixture of mulberry leaf protein and titanium dioxide powder is 3:1.

[0016] Compared with the prior art, the present invention has the following beneficial effects: The invention consists of mulberry leaf protein and titanium dioxide, wherein the mulberry leaf protein contains multiple amino acids and can improve the nutritional value of mulberry leaves. Meanwhile, the mulberry leaf protein contains alkaloids and flavonoid compounds and has antibacterial, antiviral and antioxidant effects, and can avoid the invasion and reproduction of pests and diseases and reduce the occurrence of diseases. Meanwhile, the mulberry leaf protein can be used as a carrier of titanium dioxide to help the titanium dioxide to be more evenly dispersed on the mulberry leaf surface, thereby improving the promoting effect of titanium dioxide on the photosynthesis of the mulberry leaf surface and improving the yield and quality of the mulberry leaf. Meanwhile, the mulberry leaf protein and titanium dioxide will not cause pollution to the surrounding environment and can even purify the surrounding air and repair the surrounding soil, thereby improving the practicality of the mulberry leaf foliar fertilizer. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 The present invention is a flow chart of the method for preparing mulberry leaf foliar fertilizer. DETAILED DESCRIPTION

[0018] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0019] The present invention provides the following technical scheme: a mulberry leaf foliar fertilizer, comprising the following components: mulberry leaf protein and titanium dioxide.

[0020] Mulberry leaf protein is extracted from mulberry leaves. It not only acts on mulberry leaves, but also promotes digestion and absorption of silkworms after being eaten by silkworms, thereby improving the feeding effect of silkworms.

[0021] A method for preparing mulberry leaf foliar fertilizer comprises the following steps: S1: Pick fresh mulberry leaves free of pests and diseases; S2: The harvested mulberry leaves are placed in a clean water pool for washing to remove dust, pesticide residues and other impurities on the surface; Ultrasonic equipment can be configured in the cleaning pool to perform ultrasonic cleaning through the ultrasonic wave of the ultrasonic equipment, thereby improving the cleanliness of the mulberry leaves, that is, improving the spraying effect of the mulberry leaf fertilizer; S3: placing the washed mulberry leaves in a dryer for drying to remove surface moisture; The dryer should preferably be an air-cooled dryer, and the air volume of the air-cooled dryer should be such that it does not cause impact or damage to the mulberry leaves. S4: placing the dried mulberry leaves in a grinder and grinding them to obtain mulberry leaf powder; The crusher is equipped with a filter screen, and only qualified mulberry leaves can enter the next step; S5: placing mulberry leaf powder and extraction solvent in an ultrasonic device according to a certain proportion, and performing ultrasonic treatment. The ultrasonic wave cavitation, stirring and breaking the cell wall of the mulberry leaf make the mulberry leaf protein fully released into the extraction solvent to obtain a mixed solution; During ultrasonic treatment, the ultrasonic equipment parameters should be adjusted and set according to the actual situation to avoid insufficient cell wall breakage caused by too low a setting or damage to mulberry leaf protein caused by too high a setting. S6: placing the mixed solution in a centrifuge for centrifugation to separate the supernatant and the mulberry leaf protein precipitate; S7: purifying the separated supernatant based on ion exchange resin to obtain mulberry leaf protein; S8: placing the titanium dioxide powder in a sieving machine for sieving to remove titanium dioxide powder with inconsistent particle sizes; S9: placing mulberry leaf protein and sieved titanium dioxide powder in a container according to a proportion, adding deionized water according to a proportion, stirring until the mixture is evenly mixed, and transporting the mixture to a filling machine through a pipeline; S10: The filling machine fills equal amounts into packaging bags or bottles and seals them.

[0022] The instructions for use of bagged or bottled mulberry leaf foliar fertilizer will be printed on the packaging bag or bottle after filling. When using the bagged or bottled mulberry leaf foliar fertilizer, it must be used strictly in accordance with the instructions printed on the packaging bag or bottle.

[0023] Specifically, in step S5, the extraction solvent is a sodium hydroxide solution, and the liquid-to-solid ratio of the sodium hydroxide solution is 50-100:1.

[0024] Specifically, in step S5, the adding ratio of mulberry leaf powder and extraction solvent is 1:20-25.

[0025] Specifically, in step S5, during the ultrasonic process, acid or alkaline solution is added to the mulberry leaf powder and the extraction solvent according to the pH value of the extraction solvent to adjust the pH value of the extraction solvent so that the pH value is maintained at 7-9.

[0026] Specifically, the specific steps of step S7 include: S71: slowly loading the supernatant onto the equilibrated ion exchange resin to allow the mulberry leaf protein to fully contact and bind to the medium; S72: pouring the buffer solution onto the ion exchange resin to elute the mulberry leaf protein and other impurities that are not bound to the medium on the ion exchange resin; S73: gradually increase the ion concentration of the buffer to gradually elute the mulberry leaf protein bound to the medium; S74: collecting the eluate containing mulberry leaf protein according to the elution curve and the properties of mulberry leaf protein; S75: The collected eluate containing mulberry leaf protein is subjected to ultrafiltration treatment to remove residual salts and other small molecular substances to obtain mulberry leaf protein.

[0027] Specifically, in step S9, the adding ratio of mulberry leaf protein and titanium dioxide powder is 1:2-3, and the adding ratio of deionized water to the mixture of mulberry leaf protein and titanium dioxide powder is 3:1.

[0028] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A mulberry leaf foliar fertilizer, It is characterized in that Includes the following ingredients: mulberry leaf protein and titanium dioxide.

2. A method for preparing a mulberry leaf foliar fertilizer according to claim 1, It is characterized in that The following steps are involved: S1: Pick fresh and pest-free mulberry leaves; S2: The harvested mulberry leaves are placed in a clean water pool for washing to remove dust, pesticide residues and other impurities on the surface; S3: placing the washed mulberry leaves in a dryer for drying to remove surface moisture; S4: placing the dried mulberry leaves in a grinder and grinding them to obtain mulberry leaf powder; S5: placing mulberry leaf powder and extraction solvent in an ultrasonic device according to a certain proportion, and performing ultrasonic treatment. The ultrasonic wave cavitation, stirring and breaking the cell wall of the mulberry leaf make the mulberry leaf protein fully released into the extraction solvent to obtain a mixed solution; S6: placing the mixed solution in a centrifuge for centrifugation to separate the supernatant and the mulberry leaf protein precipitate; S7: purifying the separated supernatant based on ion exchange resin to obtain mulberry leaf protein; S8: placing the titanium dioxide powder in a sieving machine for sieving to remove titanium dioxide powder with inconsistent particle sizes; S9: placing mulberry leaf protein and sieved titanium dioxide powder in a container according to a proportion, adding deionized water according to a proportion, stirring until the mixture is evenly mixed, and transporting the mixture to a filling machine through a pipeline; S10: The filling machine fills equal amounts into packaging bags or bottles and seals them.

3. A method for preparing a mulberry leaf foliar fertilizer according to claim 2, Features: In step S5, the extraction solvent is sodium hydroxide solution, and the liquid-to-solid ratio of the sodium hydroxide solution is 50-100:

1.

4. A method for preparing a mulberry leaf foliar fertilizer according to claim 2, Features: In step S5, the adding ratio of mulberry leaf powder to extraction solvent is 1:20-25.

5. A method for preparing a mulberry leaf foliar fertilizer according to claim 2, Features: In step S5, during the ultrasonic process, acid or alkaline solution is added to the mulberry leaf powder and the extraction solvent according to the pH value of the extraction solvent to adjust the pH value of the extraction solvent so that the pH value is maintained at 7-9.

6. A method for preparing a mulberry leaf foliar fertilizer according to claim 2, Features: The specific steps of step S7 include: S71: slowly loading the supernatant onto the equilibrated ion exchange resin to allow the mulberry leaf protein to fully contact and bind to the medium; S72: pouring the buffer solution onto the ion exchange resin to elute the mulberry leaf protein and other impurities that are not bound to the medium on the ion exchange resin; S73: gradually increase the ion concentration of the buffer to gradually elute the mulberry leaf protein bound to the medium; S74: collecting the eluate containing mulberry leaf protein according to the elution curve and the properties of mulberry leaf protein; S75: The collected eluate containing mulberry leaf protein is subjected to ultrafiltration treatment to remove residual salts and other small molecular substances to obtain mulberry leaf protein.

7. A method for preparing a mulberry leaf foliar fertilizer according to claim 2, Features: In the step S9, the adding ratio of mulberry leaf protein and titanium dioxide powder is 1:2-3, and the adding ratio of deionized water to the mixture of mulberry leaf protein and titanium dioxide powder is 3:1.

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