Application of 2, 4-di-tert-butylphenol in prevention and treatment of crop southern blight

Abstract

The invention discloses application of 2, 4-di-tert-butylphenol in the aspect of preventing and treating crop southern blight, belongs to the technical field of biopesticides, and provides application of 2, 4-di-tert-butylphenol in the aspect of preventing and treating crop southern blight, and crops are one or more of peanuts, Chinese chives and rhizoma atractylodis macrocephalae. The 2, 4-DTBP is found to have very strong inhibitory activity on southern blight germs derived from three crops of peanuts, Chinese chives and bighead atractylodes rhizome, has very great application potential, and can be used as a candidate drug for developing a southern blight green prevention and control drug.

Description

Technical Field

[0001] The invention belongs to the technical field of biological pesticides, and particularly relates to application of 2,4-di-tert-butylphenol in preventing and controlling white rot of crops. Background Art

[0002] Sclerotium rolfsii is a soil-borne fungal disease caused by infection with Sclerotium rolfsii, which can harm more than 500 kinds of plants. Sclerotium rolfsii can occur throughout the entire growth period of crops, mainly harming the base of the stem of the plant, causing the plant to wither and die. When the environment is humid, the mycelium in the base of the stem and the nearby soil will form brown, rapeseed-like sclerotia. The pathogen of Sclerotium rolfsii mainly overwinters in the soil in the form of sclerotia, and can also overwinter on the diseased and residual body of the plant in the form of mycelium. When the environmental conditions are suitable, the sclerotia germinates and grows hyphae, directly invading from the wound or epidermis at the base of the plant stem, causing the disease to occur; the sclerotia can also be spread by irrigation, rain, cultivation or farming operations, causing reinfection. In recent years, due to the influence of factors such as climate warming, large-scale planting and returning straw to the field, the occurrence of Sclerotium rolfsii has become more and more serious year by year. At present, the use of chemical agents is the main measure to prevent and control white rot, but there are few types of chemical agents that can effectively prevent and control white rot without side effects. At the same time, long-term and large-scale use is very likely to produce drug resistance, and will also cause environmental pollution and threaten human life and health. Therefore, it is urgent to find new, safe, sustainable and green control agents.

[0003] 2,4-di-tert-butylphenol, referred to as 2,4-DTBP, is a brown-yellow solid. It is an important intermediate for fine chemical products and is widely used as an antioxidant in plastics, rubber, fuel oil, etc. It is a common natural product and one of the secondary metabolites produced by various biological groups. It has a wide range of sources and has been found in 169 species. The research on 2,4-DTBP is mainly focused on the fields of medicine, food and agricultural pest control. Studies have found that 2,4-DTBP can play an anti-inflammatory role by inhibiting the expression of tumor necrosis factor-related genes in mouse phagocytes. At the same time, studies have also found that 2,4-DTBP has excellent contact killing activity against eggs, larvae, and adults of Tetranychus cinnabarinus, and can significantly reduce its egg-laying rate. When applied at a sublethal dose, it shows a significant repellent effect on Tetranychus cinnabarinus. The results show that it has the potential to be developed as a new natural acaricide. At present, there are no reports on the biological activity and control effect of 2,4-DTBP on Sclerotium rolfsii. Summary of the invention

[0004] In order to solve the above technical problems, the present invention proposes the use of 2,4-di-tert-butylphenol in preventing and controlling white rot of crops, providing a new, safe and sustainable green control agent for the prevention and control of white rot of crops.

[0005] To achieve the above object, the present invention provides the use of 2,4-di-tert-butylphenol in preventing and controlling white rot of crops.

[0006] Preferably, the crop is one or more of peanut, leek or Atractylodes macrocephala.

[0007] Preferably, the EC of 2,4-di-tert-butylphenol to Sclerotium rolfsii is 50 The value is 0.18~0.33mg / L.

[0008] The present invention also provides a preparation for preventing and controlling white rot of crops, comprising the 2,4-di-tert-butylphenol.

[0009] The present invention also provides the use of the 2,4-di-tert-butylphenol in the preparation of a mycelium growth inhibitor of Sclerotium rolfsii.

[0010] Preferably, the hyphae of Saccharomyces rolfsii are derived from one of peanut, leek or Atractylodes macrocephala.

[0011] The present invention also provides a mycelium growth inhibitor of Sclerotium rolfsii containing the 2,4-di-tert-butylphenol.

[0012] The present invention also provides the use of the 2,4-di-tert-butylphenol in the preparation of a sclerotium growth inhibitor for Sclerotium rolfsii.

[0013] Preferably, the sclerotia of S. rolfsii are derived from one of peanut, leek or Atractylodes macrocephala.

[0014] The present invention also provides a Sclerotium rolfsii sclerotium growth inhibitor comprising the 2,4-di-tert-butylphenol, wherein the concentration of the 2,4-di-tert-butylphenol in the Sclerotium rolfsii sclerotium growth inhibitor is 12.8-25.6 mg / L.

[0015] Compared with the prior art, the present invention has the following advantages and technical effects:

[0016] 2,4-di-tert-butylphenol has good inhibitory activity on the mycelial growth of Saccharomyces solani, and with the increase of the concentration of 2,4-di-tert-butylphenol, the inhibitory effect on the mycelium gradually increases; 2,4-di-tert-butylphenol has good inhibitory activity on the germination of sclerotia of Saccharomyces solani, and when the concentration of 2,4-di-tert-butylphenol is 25.6 mg / L, the inhibition rate of sclerotia of Saccharomyces solani from peanut and leek can reach 100%; 2,4-DTBP has strong inhibitory activity against Saccharomyces solani of three crops, peanut, leek and Atractylodes macrocephala, and has great application potential. It can be used as a candidate drug for the development of green control agents for Saccharomyces solani. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0018] Figure 1 This is a graph showing the inhibition of different concentrations of 2,4-DTBP on the mycelial growth of white rot fungus from peanut;

[0019] Figure 2 This is a graph showing the inhibition of different concentrations of 2,4-DTBP on the germination of white rot fungi from peanut. DETAILED DESCRIPTION

[0020] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as limiting the present invention, but should be understood as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0021] It should be understood that the terms described in the present invention are only for describing special embodiments and are not intended to limit the present invention. In addition, for the numerical range in the present invention, it should be understood that each intermediate value between the upper and lower limits of the scope is also specifically disclosed. Each smaller range between the intermediate value in any stated value or stated range and any other stated value or intermediate value in the described range is also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded in the scope.

[0022] Unless otherwise indicated, all technical and scientific terms used herein have the same meanings as those generally understood by those skilled in the art. Although the present invention describes only preferred methods and materials, any methods and materials similar or equivalent to those described herein may also be used in the implementation or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials associated with the documents. In the event of a conflict with any incorporated document, the content of this specification shall prevail.

[0023] It will be apparent to those skilled in the art that various modifications and variations may be made to the specific embodiments of the present invention description without departing from the scope or spirit of the present invention. Other embodiments derived from the present invention description will be apparent to the skilled artisan. The present invention description and examples are exemplary only.

[0024] The words “include,” “including,” “have,” “contain,” etc. used in this document are open-ended terms, meaning including but not limited to.

[0025] Sources of materials used in the present invention: 2,4-di-tert-butylphenol (2,4-DTBP) powder, with a content of 97%, is a commercially available commodity; and a PDA flat plate is commercially available.

[0026] Example 1

[0027] 1. Determination of the toxicity of 2,4-DTBP to the mycelial growth of S. rolfsii

[0028] The mycelium growth rate method (double dishes buckled) was used for determination. 0.1030 g of 2,4-DTBP powder was dissolved in 1 mL of a mixed solution of DMSO and Tween-80 in a volume ratio of 1:1 to prepare a 0.1 mg / μL drug solution. 0.14 μL, 0.28 μL, 0.56 μL, 1.12 μL, and 2.24 μL of the drug solution were respectively added dropwise to a PDA plate containing a filter paper (1 cm in diameter). Double dishes were buckled to prepare a total of 5 drug-containing culture media with final concentrations of 0.1 mg / L, 0.2 mg / L, 0.4 mg / L, 0.8 mg / L, and 1.6 mg / L. A drug-free culture medium with 2.24 μL of a mixed solution of DMSO and Tween-80 in a volume ratio of 1:1 was added as a control. Each concentration was repeated 3 times. Three replicates were inoculated with S. rolfsii from peanut, S. rolfsii from leek, and S. rolfsii from Atractylodes macrocephala, respectively, and cultured in a dark incubator at 25°C until the mycelium of the control group grew to 2 / 3 of the culture dish. The colony diameter was measured using the cross method. The inhibition rate of 2,4-DTBP on the mycelium growth of S. rolfsii was calculated, and the toxicity regression equation and effective median concentration (EC 50 ), the concentration with an inhibition rate of 85% (EC 85 ).

[0029] The inhibition rate of mycelium growth (%) = [(mycelium diameter of the control group - mycelium diameter of the treatment group) / (mycelium diameter of the control group - mycelium cake diameter)] × 100%. The results are shown in Table 1 below.

[0030] Table 1 Toxicity of 2,4-DTBP to mycelium of Sclerotium rolfsii

[0031] crop <![CDATA[EC 50 (mg / L)]]> Confidence interval <![CDATA[EC 85 (mg / L)]]> Confidence interval Virulence regression equation peanut 0.26 0.23～0.29 1.20 1.02～1.42 Y=5.91+1.56x Chinese chives 0.18 0.11～0.29 5.49 3.02～9.96 Y=5.52+0.70x Atractylodes macrocephala 0.33 0.26～0.42 7.07 5.00～10.00 Y=5.37+0.78x

[0032] Depend on Figure 1 The results show that when the concentration of 2,4-DTBP reaches 0.2 mg / L or more, the growth of southern rot fungus hyphae on peanuts can be significantly inhibited.

[0033] The results in Table 1 show that 2,4-DTBP has strong inhibitory activity against the mycelium of S. rolfsii on three crops: peanut, leek and Atractylodes macrocephala. 50 The value range is 0.18~0.33mg / L.

[0034] 2. Determination of the effect of 2,4-DTBP on sclerotial germination of S. rolfsii

[0035] The double-dish assay was performed, 0.1030 g of 2,4-DTBP powder was dissolved in 1 mL of a mixed solution of DMSO and Tween-80 in a volume ratio of 1:1 to prepare a 0.1 mg / μL solution, and 4.48 μL, 8.96 μL, 13.44 μL, 17.92 μL, and 35.84 μL of the solution were added dropwise to a PDA plate containing a filter paper (1 cm in diameter), and the double-dish assay was performed to prepare a final concentration of 3.2 mg / L, 6.4 mg / L, 9.6 mg / L, 12.8 mg / L, and 25.84 μL of the solution. A total of 5 kinds of drug-containing culture media were used, with 35.84 μL of a mixed solution of DMSO and Tween-80 in a volume ratio of 1:1 added, and a drug-free culture medium with 35.84 μL of DMSO and Tween-80 in a volume ratio of 1:1 was used as the control. Sclerotia of white rot were selected with the same size and shape and placed in PDA plates, 20 sclerotia were placed in each treatment, and each concentration was repeated 3 times. Sclerotia of white rot from peanut, leek and Atractylodes macrocephala were placed in 3 replicates, and after culturing in the dark at 25°C in an incubator for 2 days, the germination of sclerotia was observed. Under a microscope, the germination was considered to be achieved when the hyphae produced by the sclerotia were ≥2 mm.

[0036] Sclerotium germination inhibition rate (%) = (the number of sclerotium germination in the control group - the number of sclerotium germination in the treatment group) / (the number of sclerotium germination in the control group) × 100%. The results are shown in Table 2 below.

[0037] Table 22,4-DTBP inhibits germination of sclerotia of Sclerotium rolfsii

[0038]

[0039] Depend on Figure 2 The results show that when the concentration of 2,4-DTBP reaches 6.4 mg / L or more, the germination of S. rolfsii fungi on peanuts can be significantly inhibited.

[0040] From the results recorded in Table 2, it can be seen that 2,4-DTBP has a good inhibitory effect on the germination of sclerotia of white rot fungus from three crops. When the concentration of 2,4-DTBP is 12.8 mg / L, the inhibition rate of 2,4-DTBP on sclerotia germination can reach more than 80%. When the concentration rises to 25.6 mg / L, it can almost completely inhibit the germination of sclerotia.

[0041] Based on the above experimental results on the inhibitory effects on the hyphae and sclerotia of Saccharomyces solani, it is proved that the natural product 2,4-DTBP has a strong inhibitory activity against Saccharomyces solani from three crops: peanut, leek and Atractylodes macrocephala. It has great application potential and can be used as a candidate drug for the development of green control agents for Saccharomyces solani.

[0042] The embodiments described above are only descriptions of the preferred modes of the present invention, and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by ordinary technicians in this field should all fall within the protection scope determined by the claims of the present invention.

Claims

1. Application of 2,4-di-tert-butylphenol in preventing and controlling white rot of crops.

2. The application according to claim 1, characterized in that: The crops are one or more of peanuts, leeks or Atractylodes macrocephala.

3. The application according to claim 1, characterized in that: The EC of 2,4-di-tert-butylphenol against Sclerotium rolfsii 50 The value is 0.18~0.33mg / L.

4. A preparation for controlling white rot of crops comprising the 2,4-di-tert-butylphenol according to claim 1.

5. Use of 2,4-di-tert-butylphenol as claimed in claim 1 in the preparation of a mycelium growth inhibitor of Sclerotium rolfsii.

6. The use according to claim 5, characterized in that: The hyphae of the Saccharomyces rolfsii fungus are derived from one of peanuts, leeks or Atractylodes macrocephala. 7 . A mycelial growth inhibitor of Sclerotium rolfsii comprising the 2,4-di-tert-butylphenol according to claim 1 .

8. Use of 2,4-di-tert-butylphenol as claimed in claim 1 in the preparation of a sclerotial growth inhibitor of Sclerotium rolfsii.

9. The use according to claim 8, characterized in that: The sclerotium of the white rot fungus is derived from one of peanut, leek or Atractylodes macrocephala.

10. A Sclerotium rolfsii sclerotium growth inhibitor comprising the 2,4-di-tert-butylphenol as claimed in claim 1, characterized in that: The concentration of 2,4-di-tert-butylphenol in the Sclerotium rolfsii sclerotium growth inhibitor is 12.8-25.6 mg / L.

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