Astaxanthin composition as well as capsule product and preparation method thereof

Abstract

The invention provides an astaxanthin composition as well as a capsule product and a preparation method thereof, and the astaxanthin composition is prepared from the following raw materials in parts by weight: 1.8 to 2.8 parts of astaxanthin oil, 1.2 to 3.2 parts of lutein ester, 1 to 3 parts of semen ziziphi spinosae powder, 1 to 3 parts of black chokeberry fruit powder, 1 to 3 parts of white kidney bean extract, 1 to 3 parts of grape powder, 2 to 4 parts of DHA algal oil and 0.5 to 1.5 parts of soybean phospholipid. 2-4 parts of wheat germ oil, 5-8 parts of olive oil, 0.1-0.3 part of vitamin C, 4-6 parts of beewax, 3-5 parts of mono / diglycerol fatty acid ester and 0.1-0.3 part of mogroside. The astaxanthin composition disclosed by the invention is not easy to oxidize and inactivate. The biological activity utilization rate is high, the active components are better absorbed and utilized, and the human health is promoted. The astaxanthin composition disclosed by the invention can resist inflammation, oxidation and aging, whiten skin, improve sleep, reduce blood fat and blood sugar and comprehensively improve body functions, and is deeply loved by consumers.

Description

Technical Field

[0001] The present invention relates to the field of health care products, and in particular to an astaxanthin composition and a capsule product and a preparation method thereof. Background Art

[0002] Astaxanthin, 3,3′-dihydroxy-4,4′-diketo-β,β′-carotene, is a terpene unsaturated compound with a chemical formula of C 40 H 52 O4, molecular weight is 596.86, natural astaxanthin is one of the strongest natural antioxidants in the world, effectively removes oxygen free radicals in cells, enhances cell regeneration, maintains body balance and reduces the accumulation of senescent cells, protects cell and DNA health from the inside out, thereby protecting skin health, promoting hair growth, anti-aging, relieving sports fatigue, and enhancing vitality. Astaxanthin has strong antioxidant activity and plays a positive role in improving immunity, preventing the occurrence and development of chronic diseases such as tumors, cardiovascular diseases, and diabetes, and delaying aging.

[0003] However, astaxanthin is a hydrophobic substance that is not resistant to high temperature and pressure. It is easily oxidized and unstable in the environment, so the application of astaxanthin is limited. Astaxanthin is prepared into different dosage forms (tablets, oral liquids, injections, capsules, etc.), but tablets are easily oxidized and degraded, affecting biological activity and stability; the cost of injections is relatively high; oral liquids need to be stored at low temperature and away from light, with poor stability, and there is a risk of oil-water separation in later products. The current capsule preparations of astaxanthin are generally soft capsules or microcapsules. The general problems of capsules are: after entering the body and disintegrating in the stomach, they are decomposed by strong acids, destroying the activity of astaxanthin, so the utilization efficiency of the active components is low. At the same time, it is necessary to further improve the stability of the capsules. Summary of the invention

[0004] In view of this, the present invention proposes an astaxanthin composition and a capsule product and a preparation method thereof to solve the above problems.

[0005] The technical solution of the present invention is achieved in this way:

[0006] An astaxanthin composition comprises the following raw materials in parts by weight: 1.8-2.8 parts of astaxanthin oil, 1.2-3.2 parts of lutein ester, 1-3 parts of jujube seed powder, 1-3 parts of aronia nigra fruit powder, 1-3 parts of white kidney bean extract, 1-3 parts of grape powder, 2-4 parts of DHA algae oil, 0.5-1.5 parts of soybean lecithin, 2-4 parts of wheat germ oil, 5-8 parts of olive oil, 0.1-0.3 parts of vitamin C, 4-6 parts of beeswax, 3-5 parts of mono- and di-glycerol fatty acid esters, and 0.1-0.3 parts of mogroside.

[0007] Furthermore, the preparation method of astaxanthin oil includes: placing Haematococcus pluvialis in an ultrasonic positioning wall breaking instrument for treatment, adding an ethanol-ammonium sulfate two-phase aqueous system, ultrasonic extraction, collecting the extract, subjecting the extract to supercritical CO2 extraction, and concentrating the extract under reduced pressure to obtain astaxanthin oil.

[0008] Furthermore, the power of the ultrasonic positioning wall breaking instrument is 400-500W; the solid-liquid ratio of the Haematococcus pluvialis and the ethanol-ammonium sulfate two-phase aqueous system is 1:4-6g / mL; the concentration of the ethanol-ammonium sulfate two-phase aqueous system is 20%-40% v / v, wherein the volume of ethanol and ammonium sulfate is 1:3-5; the ultrasonic extraction is performed at a power of 200-300W and a temperature of 40-50°C for 25-35min; the supercritical CO2 extraction has a pressure of 20-40MPa, a temperature of 35-45°C, a flow rate of 5-7mL / min, and a time of 1-3h.

[0009] Furthermore, the preparation method of the white kidney bean extract includes: adding white kidney bean powder to water, first performing microwave extraction, collecting the extract, adding the residue to a 70%-80% v / v ethanol solution, then performing ultrasonic extraction, collecting the extract, combining the two extracts, and vacuum concentrating to obtain the white kidney bean extract.

[0010] Furthermore, the solid-liquid ratio of the white kidney bean powder to water is 1:8-10 g / mL; the microwave extraction is performed at 50-60°C and 400-600W for 30-60 min; the solid-liquid ratio of the residue to the ethanol solution is 1:4-6 g / mL; and the ultrasonic extraction is performed at 20-30KHz and 100-200W for 40-60 min.

[0011] A method for preparing an astaxanthin composition, the specific steps comprising:

[0012] (1) mixing astaxanthin oil, lutein ester, DHA algae oil, soybean lecithin, wheat germ oil, and olive oil, stirring in a high-pressure homogenizer to obtain an oily composition, adding mono- and di-glycerol fatty acid esters to the oily composition, and stirring at high speed to obtain an oily emulsion;

[0013] (2) adding the jujube seed powder, Aronia nigra fruit powder, white kidney bean extract, grape powder and vitamin C into water, and stirring with ultrasound to obtain an aqueous composition;

[0014] (3) Mixing the aqueous composition and the oily emulsion, adding beeswax, stirring at a low speed, and adding mogroside to obtain the target astaxanthin composition.

[0015] Furthermore, in step (1), the high-pressure homogenizer is: pressure 30-50 MPa, time 5-10 min; the high-speed shear stirring is stirring at 3000-4000 rpm for 10-15 min;

[0016] In step (2), the amount of water added is 6-8 times the total weight of the wild jujube seed powder, black fruit glandular rib fruit powder, white kidney bean extract, grape powder and vitamin C; the ultrasonic stirring: power 30-50KHz, time 30-60min;

[0017] In step (3), the low-speed stirring is at a speed of 50-150 rpm and a time of 1-3 h.

[0018] An astaxanthin capsule product comprises the astaxanthin composition and a capsule shell.

[0019] Furthermore, the capsule shell comprises the following raw materials in parts by weight: 1-3 parts of gelatin, 3-7 parts of glycerin, 0.01-0.03 parts of caramel color, and 0.05-0.08 parts of xylitol.

[0020] A method for preparing an astaxanthin capsule product, the specific steps comprising:

[0021] (1) heating and stirring gelatin and xylitol at a temperature of 50-70° C. and a speed of 200-300 rpm for 30-40 minutes, adding glycerin, continuing to stir for 10-20 minutes, adding caramel color, stirring evenly, filtering, defoaming, and forming to obtain a capsule shell;

[0022] (2) injecting the astaxanthin composition into a capsule shell to obtain the target astaxanthin capsule product.

[0023] Compared with the prior art, the present invention has the following beneficial effects:

[0024] 1. The astaxanthin composition of the present invention has good stability and is not easily oxidized and inactivated. The biological activity utilization rate is high, and the active components are better absorbed and utilized to promote human health. The astaxanthin composition of the present invention can resist inflammation, oxidation, anti-aging, whiten, improve sleep, reduce blood lipids and blood sugar, and comprehensively improve body functions, and is deeply loved by consumers.

[0025] 2. The DHA algae oil and white kidney bean extract in the astaxanthin composition of the present invention can enhance the blood lipid and blood sugar lowering effects of astaxanthin, the spiny jujube seed powder and the black fruit aronia fruit powder enhance the anti-inflammatory, antioxidant, anti-aging and whitening effects of astaxanthin, and the wheat germ oil and olive oil of the present invention can enhance the solubility of astaxanthin. The formed astaxanthin composition has high stability, high biological activity utilization rate, and high component utilization rate.

[0026] 3. The astaxanthin capsule product prepared by the present invention has good stability and simple process operation. The capsule shell can control the release rate of the astaxanthin composition to achieve a sustained release effect, making the astaxanthin composition less prone to oxidation and inactivation, thereby improving the stability of the capsule product.

[0027] 4. The present invention uses ultrasonic cell wall breaking treatment to gently and efficiently destroy the cell wall, combines ultrasonic extraction with an ethanol-ammonium sulfate aqueous two-phase system to highly selectively extract astaxanthin active substances and improve the solubility of astaxanthin. The extract is subjected to supercritical CO2 extraction to obtain high-purity astaxanthin oil. DETAILED DESCRIPTION

[0028] In order to better understand the technical content of the present invention, specific embodiments are provided below to further illustrate the present invention.

[0029] Unless otherwise specified, the experimental methods used in the embodiments of the present invention are all conventional methods.

[0030] Unless otherwise specified, the materials, reagents, etc. used in the embodiments of the present invention can be obtained from commercial sources.

[0031] Example 1

[0032] An astaxanthin composition comprises the following raw materials in parts by weight: 2.3 parts of astaxanthin oil, 2.2 parts of lutein esters, 2 parts of jujube seed powder, 2 parts of aronia nigra fruit powder, 2 parts of white kidney bean extract, 2 parts of grape powder, 3 parts of DHA algae oil, 1 part of soybean lecithin, 3 parts of wheat germ oil, 7 parts of olive oil, 0.2 parts of vitamin C, 5 parts of beeswax, 4 parts of mono- and di-glycerol fatty acid esters, and 0.2 parts of mogroside.

[0033] Preparation of astaxanthin oil: Place Haematococcus pluvialis in a 450W ultrasonic positioning wall breaking instrument for treatment, add it to a 30% v / v ethanol-ammonium sulfate two-phase aqueous system (the volume of ethanol and ammonium sulfate is 1:4) according to a solid-liquid ratio of 1:5g / mL, and ultrasonically extract for 30 minutes at a power of 250W and a temperature of 45°C. Collect the extract, extract it with supercritical CO2 under the conditions of 30MPa, 40°C, and 6mL / min for 2h, and concentrate the extract under reduced pressure to obtain astaxanthin oil.

[0034] Preparation of white kidney bean extract: add white kidney bean powder to water at a solid-liquid ratio of 1:9 g / mL, microwave extraction at 55°C, 500 W for 45 min, collect the extract, add the residue to 75% v / v ethanol solution at a solid-liquid ratio of 1:5 g / mL, ultrasonic extraction at 25 KHz, 150 W for 50 min, collect the extract, combine the two extracts, and concentrate in vacuo to obtain white kidney bean extract.

[0035] The preparation method of the astaxanthin composition comprises the following specific steps:

[0036] (1) astaxanthin oil, lutein ester, DHA algae oil, soybean lecithin, wheat germ oil and olive oil were mixed, stirred in a high-pressure homogenizer at a pressure of 40 MPa for 8 minutes to obtain an oily composition, mono- and di-glycerol fatty acid esters were added to the oily composition, and sheared and stirred at 3500 rpm for 13 minutes to obtain an oily emulsion;

[0037] (2) adding the jujube seed powder, aronia oleracea fruit powder, white kidney bean extract, grape powder and vitamin C into water (the amount of water added is 7 times the total weight of the jujube seed powder, aronia oleracea fruit powder, white kidney bean extract, grape powder and vitamin C), and stirring ultrasonically at a power of 40 kHz for 45 minutes to obtain an aqueous composition;

[0038] (3) The aqueous composition and the oily emulsion were mixed, beeswax was added, and the mixture was stirred at a speed of 100 rpm for 2 h. Mogroside was added to obtain the target astaxanthin composition.

[0039] An astaxanthin capsule product comprises the astaxanthin composition and a capsule shell. The capsule shell comprises the following raw materials in parts by weight: 2 parts of gelatin, 5 parts of glycerin, 0.02 parts of caramel color, and 0.07 parts of xylitol.

[0040] The specific preparation method of the above-mentioned astaxanthin capsule product comprises:

[0041] (1) gelatin and xylitol are mixed, stirred at 60° C. and 250 rpm for 35 min, glycerin is added and stirring is continued for 15 min, caramel color is added, stirred evenly, filtered, degassed, and formed to obtain a capsule shell;

[0042] (2) injecting the astaxanthin composition into a capsule shell to obtain the target astaxanthin capsule product.

[0043] Example 2

[0044] An astaxanthin composition comprises the following raw materials in parts by weight: 1.8 parts of astaxanthin oil, 1.2 parts of lutein ester, 1 part of jujube seed powder, 1 part of aronia nigra fruit powder, 1 part of white kidney bean extract, 1 part of grape powder, 2 parts of DHA algae oil, 0.5 parts of soybean lecithin, 2 parts of wheat germ oil, 5 parts of olive oil, 0.1 part of vitamin C, 4 parts of beeswax, 3 parts of mono- and di-glycerol fatty acid esters, and 0.1 part of mogroside.

[0045] Preparation of astaxanthin oil: Place Haematococcus pluvialis in a 400W ultrasonic positioning wall breaking instrument for treatment, add it to an ethanol-ammonium sulfate two-phase aqueous system (the volume of ethanol and ammonium sulfate is 1:3) with a solid-liquid ratio of 1:4g / mL, and perform ultrasonic extraction at a power of 200W and a temperature of 40°C for 25min. Collect the extract, extract it with supercritical CO2 under the conditions of 20MPa, 35°C, and 5mL / min for 1h, and concentrate the extract under reduced pressure to obtain astaxanthin oil.

[0046] Preparation of white kidney bean extract: add white kidney bean powder to water at a solid-liquid ratio of 1:8 g / mL, microwave extraction at 50°C, 400 W for 30 min, collect the extract, add the residue to 70% v / v ethanol solution at a solid-liquid ratio of 1:4 g / mL, ultrasonic extraction at 20 KHz, 100 W for 40 min, collect the extract, combine the two extracts, and concentrate in vacuo to obtain the white kidney bean extract.

[0047] The preparation method of the astaxanthin composition comprises the following specific steps:

[0048] (1) astaxanthin oil, lutein ester, DHA algae oil, soybean lecithin, wheat germ oil and olive oil were mixed, stirred in a high-pressure homogenizer at a pressure of 30 MPa for 5 minutes to obtain an oily composition, mono- and diglycerol fatty acid esters were added to the oily composition, and sheared and stirred at 3000 rpm for 10 minutes to obtain an oily emulsion;

[0049] (2) adding the jujube seed powder, aronia oleracea fruit powder, white kidney bean extract, grape powder and vitamin C into water (the amount of water added is 6 times the total weight of the jujube seed powder, aronia oleracea fruit powder, white kidney bean extract, grape powder and vitamin C), and stirring ultrasonically at a power of 30 kHz for 30 min to obtain an aqueous composition;

[0050] (3) The aqueous composition and the oily emulsion were mixed, beeswax was added, and the mixture was stirred at a speed of 50 rpm for 1 h. Mogroside was added to obtain the target astaxanthin composition.

[0051] An astaxanthin capsule product comprises the astaxanthin composition and a capsule shell. The capsule shell comprises the following raw materials in parts by weight: 1 part of gelatin, 3 parts of glycerin, 0.01 part of caramel color, and 0.05 part of xylitol.

[0052] The specific preparation method of the above-mentioned astaxanthin capsule product comprises:

[0053] (1) gelatin and xylitol are mixed, stirred at a temperature of 50° C. and a speed of 200 rpm for 30 min, glycerin is added, stirring is continued for 10 min, caramel color is added, stirring is uniform, filtering, degassing, and forming to obtain a capsule shell;

[0054] (2) injecting the astaxanthin composition into a capsule shell to obtain an astaxanthin capsule product.

[0055] Example 3

[0056] An astaxanthin composition comprises the following raw materials in parts by weight: 2.8 parts of astaxanthin oil, 3.2 parts of lutein ester, 3 parts of jujube seed powder, 3 parts of aronia nigra fruit powder, 3 parts of white kidney bean extract, 3 parts of grape powder, 4 parts of DHA algae oil, 1.5 parts of soybean lecithin, 4 parts of wheat germ oil, 8 parts of olive oil, 0.3 parts of vitamin C, 6 parts of beeswax, 5 parts of mono- and di-glycerol fatty acid esters, and 0.3 parts of mogroside.

[0057] Preparation of astaxanthin oil: Place Haematococcus pluvialis in a 500W ultrasonic positioning wall breaking instrument for treatment, add it to a 40% v / v ethanol-ammonium sulfate two-phase aqueous system (the volume of ethanol and ammonium sulfate is 1:5) according to a solid-liquid ratio of 1:6g / mL, and ultrasonically extract for 35 minutes at a power of 300W and a temperature of 50°C. Collect the extract, extract it with supercritical CO2 under the conditions of 40MPa, 45°C, and 7mL / min for 3h, and concentrate the extract under reduced pressure to obtain astaxanthin oil.

[0058] Preparation of white kidney bean extract: add white kidney bean powder to water at a solid-liquid ratio of 1:10 g / mL, microwave extraction at 60°C, 600 W for 60 min, collect the extract, add the residue to 80% v / v ethanol solution at a solid-liquid ratio of 1:6 g / mL, ultrasonic extraction at 30 KHz, 200 W for 60 min, collect the extract, combine the two extracts, and concentrate in vacuo to obtain the white kidney bean extract.

[0059] The preparation method of the astaxanthin capsules comprises the following specific steps:

[0060] (1) astaxanthin oil, lutein ester, DHA algae oil, soybean lecithin, wheat germ oil and olive oil were mixed, stirred in a high-pressure homogenizer at a pressure of 50 MPa for 10 min to obtain an oily composition, mono- and diglycerol fatty acid esters were added to the oily composition, and sheared and stirred at 4000 rpm for 15 min to obtain an oily emulsion;

[0061] (2) adding the jujube seed powder, aronia oleracea fruit powder, white kidney bean extract, grape powder and vitamin C into water (the amount of water added is 8 times the total weight of the jujube seed powder, aronia oleracea fruit powder, white kidney bean extract, grape powder and vitamin C), and stirring ultrasonically at a power of 50 kHz for 60 minutes to obtain an aqueous composition;

[0062] (3) The aqueous composition and the oily emulsion were mixed, beeswax was added, and the mixture was stirred at a speed of 150 rpm for 3 h, and mogroside was added to obtain the target astaxanthin composition.

[0063] An astaxanthin capsule product comprises the astaxanthin composition and a capsule shell, wherein the capsule shell comprises the following raw materials in parts by weight: 3 parts of gelatin, 7 parts of glycerin, 0.03 parts of caramel color, and 0.08 parts of xylitol.

[0064] The specific preparation method of the above-mentioned astaxanthin capsule product comprises:

[0065] (1) gelatin and xylitol are mixed, stirred at 70° C. and 300 rpm for 40 min, glycerin is added, stirring is continued for 20 min, caramel color is added, stirring is uniform, filtering, degassing, and forming to obtain a capsule shell;

[0066] (2) injecting the astaxanthin composition into a capsule shell to obtain the target astaxanthin capsule product.

[0067] Comparative Example 1

[0068] The difference from Example 1 is that the amount of raw materials is different, and the rest is consistent with Example 1.

[0069] The astaxanthin composition of this comparative example includes the following raw materials in parts by weight: 1.5 parts of astaxanthin oil, 1 part of lutein ester, 4 parts of jujube seed powder, 0.5 part of aronia suffruticosa fruit powder, 0.8 part of white kidney bean extract, 0.7 part of grape powder, 5 parts of DHA algae oil, 0.4 part of soybean lecithin, 1 part of wheat germ oil, 4 parts of olive oil, 0.4 part of vitamin C, 3 parts of beeswax, 2.8 parts of mono- and diglycerol fatty acid esters, and 0.09 part of mogroside.

[0070] The astaxanthin capsule preparation of this comparative example comprises the above-mentioned astaxanthin composition and a capsule shell. The capsule shell comprises the following raw materials in parts by weight: 4 parts of gelatin, 8 parts of glycerin, 0.05 parts of caramel color, and 0.04 parts of xylitol.

[0071] Comparative Example 2

[0072] The difference from Example 1 is that the astaxanthin composition lacks DHA algae oil, and the weight of the missing component is allocated to other components according to the ratio of DHA algae oil, white kidney bean extract and astaxanthin oil. The rest is consistent with Example 1.

[0073] The step (1) of the preparation method of the astaxanthin composition of this comparative example is specifically as follows: (1) astaxanthin oil, lutein ester, soybean lecithin, wheat germ oil and olive oil are mixed, and stirred in a high-pressure homogenizer at a pressure of 40 MPa for 8 minutes to obtain an oily composition, mono- and di-glycerol fatty acid esters are added to the oily composition, and shear-stirred at 3500 rpm for 13 minutes to obtain an oily emulsion.

[0074] Comparative Example 3

[0075] The difference from Example 1 is that the astaxanthin composition lacks white kidney bean extract, and the weight of the missing component is allocated to other components according to the ratio of DHA algae oil, white kidney bean extract and astaxanthin oil. The rest is consistent with Example 1.

[0076] The step (2) of the preparation method of the astaxanthin composition of this comparative example is specifically as follows: (2) adding sour jujube seed powder, aronia nigra fruit powder, grape powder and vitamin C into water (the amount of water added is 7 times the total weight of sour jujube seed powder, aronia nigra fruit powder, grape powder and vitamin C), and ultrasonically stirring at a power of 40KHz for 45 minutes to obtain an aqueous composition.

[0077] Comparative Example 4

[0078] The difference from Example 1 is that the preparation process of the white kidney bean extract in the astaxanthin composition is first ultrasonic extraction and then microwave extraction, and the rest is consistent with Example 1.

[0079] The preparation method of the white kidney bean extract of this comparative example is as follows: adding white kidney bean powder to water at a solid-liquid ratio of 1:9 g / mL, ultrasonically extracting at 25 KHz, 150 W for 50 min, collecting the extract, adding the residue to a 75% v / v ethanol solution at a solid-liquid ratio of 1:5 g / mL, microwave extracting at 55° C., 500 W for 45 min, collecting the extract, combining the two extracts, and concentrating in vacuo to obtain the white kidney bean extract.

[0080] Comparative Example 5

[0081] The difference from Example 1 is that in the preparation process of the astaxanthin oil in the astaxanthin composition, the ethanol-ammonium sulfate two-phase aqueous system is replaced with an ethanol solution with a concentration of 30% v / v, and the rest is consistent with Example 1.

[0082] The astaxanthin oil of this comparative example is prepared as follows: Haematococcus pluvialis is placed in a 450W ultrasonic positioning wall breaking instrument for treatment, added into an ethanol solution with a concentration of 30% v / v according to a solid-liquid ratio of 1:5 g / mL, ultrasonically extracted at a power of 250 W and a temperature of 45°C for 30 min, the extract is collected, and the extract is extracted with supercritical CO2 at 30 MPa, 40°C, and 6 mL / min for 2 h, and the extract is concentrated under reduced pressure to obtain astaxanthin oil.

[0083] Comparative Example 6

[0084] The difference from Example 1 is that the content of astaxanthin oil in the astaxanthin composition is 1 part, and the rest is consistent with Example 1.

[0085] That is, an astaxanthin composition, which includes the following raw materials in parts by weight: 1 part of astaxanthin oil, 2.2 parts of lutein esters, 2 parts of jujube seed powder, 2 parts of aronia suffruticosa fruit powder, 2 parts of white kidney bean extract, 2 parts of grape powder, 3 parts of DHA algae oil, 1 part of soybean lecithin, 3 parts of wheat germ oil, 7 parts of olive oil, 0.2 parts of vitamin C, 5 parts of beeswax, 4 parts of mono- and diglycerol fatty acid esters, and 0.2 parts of mogroside.

[0086] Test example - lowering blood lipids and blood sugar

[0087] Hyperglycemia and hyperlipidemia modeling method: 130 clean rats (weight 200±10g) were purchased and randomly divided into 10 control groups and 120 modeling groups according to their weight. The modeling group was fed with a high-fat and high-sugar diet, while the control group was fed with a basic diet. After one month of feeding, streptozotocin solution (40mg / kg) was injected into the abdominal cavity of the modeling group rats, and the control group was injected with the same amount of 0.1mol / L buffer solution (citric acid-sodium citrate pH=4.4).

[0088] Administration method: After blood lipid and blood sugar levels were stabilized, 120 modeling groups were randomly divided into 12 groups (model group, Example 1-3 group, Comparative Example 1-6 group and commercial group) according to body weight, with 10 in each group. The modeling group continued to be fed with high-fat and high-sugar feed, the control group was fed with basic feed, the Example 1-3 group and the Comparative Example 1-6 group were given corresponding astaxanthin capsules, the commercial group was given commercially available astaxanthin capsules, twice a day for 4 consecutive weeks, and the control group and the model group were given 0.9% NaCl solution by gavage, twice a day for 4 consecutive weeks.

[0089] Test index: Rats were anesthetized by intraperitoneal injection of 2% sodium pentobarbital (45 mg / kg), and blood was collected from the abdominal aorta. The blood was naturally coagulated for 15 minutes at room temperature and centrifuged for 20 minutes (2500 rpm). The upper transparent serum was collected, and the insulin concentration was detected by ELISA, and the blood lipid level in the serum (total cholesterol, low-density lipoprotein cholesterol, triglycerides) was detected. The results are shown in Table 1.

[0090] Table 1

[0091]

[0092]

[0093] As shown in Table 1, the astaxanthin capsule products prepared in Examples 1-3 of the present invention can improve insulin resistance in diabetic rats, reduce blood sugar and blood lipids, and are significantly better than the astaxanthin capsules of the commercially available group. Compared with Example 1, the effects of reducing blood sugar and blood lipids in Comparative Examples 1-6 are reduced.

[0094] Comparative Example 7

[0095] The difference from Example 1 is that the astaxanthin composition lacks jujube seed powder, and the weight of the missing component is allocated to other components according to the ratio of jujube seed powder, Aronia nigra fruit powder and astaxanthin oil. The rest is consistent with Example 1.

[0096] The step (2) of the preparation method of the astaxanthin composition of this comparative example is specifically as follows: (2) adding Aronia nigra fruit powder, white kidney bean extract, grape powder and vitamin C into water (the amount of water added is 7 times the total weight of the Ziziphus jujuba seed powder, Aronia nigra fruit powder, white kidney bean extract, grape powder and vitamin C), and ultrasonically stirring at a power of 40 kHz for 45 minutes to obtain an aqueous composition.

[0097] Comparative Example 8

[0098] The difference from Example 1 is that the astaxanthin composition lacks Aronia nigromaculata fruit powder, and the weight of the missing component is allocated to other components according to the ratio of Ziziphus jujuba seed powder, Aronia nigromaculata fruit powder and astaxanthin oil. The rest is consistent with Example 1.

[0099] The step (2) of the preparation method of the astaxanthin composition of this comparative example is specifically as follows: (2) adding the jujube seed powder, white kidney bean extract, grape powder and vitamin C into water (the amount of water added is 7 times the total weight of the jujube seed powder, aronia nigra fruit powder, white kidney bean extract, grape powder and vitamin C), and stirring ultrasonically at a power of 40KHz for 45 minutes to obtain an aqueous composition.

[0100] Test example

[0101] 1. DPPH free radical scavenging rate

[0102] Experimental method: Prepare DPPH solution (0.1mmol / L) with anhydrous ethanol and store it in the dark. Prepare the test sample into a solution with a concentration of 0.5mg / mL. Pipette 2mL of the test sample solution and 2mL of the DPPH solution into the same test tube, shake well, and let it stand at room temperature in the dark for 30 minutes. The absorbance is recorded as A1; after mixing 2mL of the DPPH solution with 2mL of distilled water, the absorbance is recorded as A2; after mixing 2mL of the test sample solution with 2mL of anhydrous ethanol, the absorbance is recorded as A3;

[0103] DPPH free radical scavenging rate (%) = [1-(A1-A3) / A2] × 100%

[0104] The test results are shown in Table 2.

[0105] Table 2

[0106] sample DPPH free radical scavenging rate Example 1 39％ Comparative Example 6 19％ Comparative Example 7 34％ Comparative Example 8 35％ Commercially available group 36％

[0107] As can be seen from Table 2, the DPPH radical scavenging rate of the astaxanthin capsule product prepared in Example 1 of the present invention is 39%, indicating that the prepared astaxanthin capsule has antioxidant capacity and is superior to the commercially available group. Compared with Example 1, the astaxanthin oil content of Comparative Example 6 is decreased, and its DPPH radical scavenging rate is decreased. Comparative Examples 7-8 lack spiny jujube seed powder and aronia melanocarpa fruit powder, and their DPPH radical scavenging rates are also decreased.

[0108] 2. Tyrosinase activity test

[0109] Experimental method: Human A-375 melanocytes in logarithmic growth phase were taken and the cell concentration was adjusted to 2×10 4 / mL, inoculated in 96-well plates (100 μL per well),

[0110] Experimental group: After 24 h, 100 μL of the sample to be tested was added and cultured for another 3 days;

[0111] Blank control group: blank complete medium;

[0112] Set up 3 replicates for each well. After culturing to the time point, wash with PBS (pH = 6.8), add 90μL of PBS (containing 1% TritonX-100) to each well, oscillate for 5 minutes, and after pre-warming at 37°C, add 10μL of 1% L-Dopa to each well. Incubate at 37°C for 40 minutes and measure the absorbance value (wavelength 492nm). Tyrosinase inhibition rate (%) = (1-average absorbance value of the sample group to be tested / average absorbance value of the control group) × 100%

[0113] The test results are shown in Table 3.

[0114] Table 3

[0115] sample Tyrosinase inhibition rate Example 1 43％ Comparative Example 6 17％ Comparative Example 7 30％ Comparative Example 8 32％ Commercially available group 35％

[0116] As shown in Table 3, the astaxanthin capsule product of Example 1 of the present invention has the effect of inhibiting tyrosinase activity, indicating that it has a good whitening and freckle removal effect, and is better than the commercially available group. The content of astaxanthin oil in Comparative Example 6 is reduced, and the effect of inhibiting tyrosinase activity is reduced. Comparative Examples 7-8 lack spiny jujube seed powder and black fruit glandular rib fruit powder, and the effect of inhibiting tyrosinase activity is reduced.

[0117] Comparative Example 9

[0118] The difference from Example 1 is that the astaxanthin composition lacks wheat germ oil and the weight of the component is not allocated to olive oil. The rest is consistent with Example 1.

[0119] The step (1) of the preparation method of the astaxanthin composition of this comparative example is specifically as follows: (1) astaxanthin oil, lutein ester, DHA algae oil, soybean lecithin and olive oil are mixed, and stirred in a high-pressure homogenizer at a pressure of 40 MPa for 8 minutes to obtain an oily composition, mono- and di-glycerol fatty acid esters are added to the oily composition, and shear-stirred at 3500 rpm for 13 minutes to obtain an oily emulsion.

[0120] Comparative Example 10

[0121] The difference from Example 1 is that the astaxanthin composition lacks olive oil, and the weight of the missing component is allocated to wheat germ oil. The rest is consistent with Example 1.

[0122] The step (1) of the preparation method of the astaxanthin composition of this comparative example is specifically as follows: (1) astaxanthin oil, lutein ester, DHA algae oil, soybean lecithin and wheat germ oil are mixed, and stirred in a high-pressure homogenizer at a pressure of 40 MPa for 8 minutes to obtain an oily composition, mono- and di-glycerol fatty acid esters are added to the oily composition, and shear-stirred at 3500 rpm for 13 minutes to obtain an oily emulsion.

[0123] Test example - stability test

[0124] The astaxanthin capsule products of Example 1, Comparative Examples 9-10 were placed in an electric constant temperature incubator at 40-50°C for 30 days, and samples were taken. After natural cooling at room temperature, the properties were observed. After refrigeration at 0°C for two weeks, the contents were sampled and the properties were observed. The results are shown in Table 4.

[0125] Table 4

[0126]

[0127] It can be seen from Table 4 that the astaxanthin capsule product prepared in Example 1 of the present invention has good stability and is not easily oxidized and inactivated. Comparative Examples 9-10 show that the stability of the capsule product and the solubility of astaxanthin will decrease in the absence of wheat germ oil or olive oil.

[0128] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. An astaxanthin composition, It is characterized in that According to parts by weight, the following raw materials are included: 1.8-2.8 parts of astaxanthin oil, 1.2-3.2 parts of lutein esters, 1-3 parts of jujube kernel powder, 1-3 parts of black fruit aronia fruit powder, 1-3 parts of white kidney bean extract, 1-3 parts of grape powder, 2-4 parts of DHA algae oil, 0.5-1.5 parts of soybean lecithin, 2-4 parts of wheat germ oil, 5-8 parts of olive oil, 0.1-0.3 parts of vitamin C, 4-6 parts of beeswax, 3-5 parts of mono- and di-glycerol fatty acid esters, and 0.1-0.3 parts of mogroside.

2. An astaxanthin composition according to claim 1, It is characterized in that The preparation method of astaxanthin oil comprises: placing Haematococcus pluvialis in an ultrasonic positioning wall breaking instrument for treatment, adding an ethanol-ammonium sulfate two-phase aqueous system, ultrasonic extraction, collecting the extract, and subjecting the extract to supercritical CO 2 Extraction, and concentration of the extract under reduced pressure to obtain astaxanthin oil.

3. An astaxanthin composition according to claim 2, It is characterized in that The power of the ultrasonic positioning wall breaking instrument is 400-500W; the solid-liquid ratio of the Haematococcus pluvialis to the ethanol-ammonium sulfate two-phase aqueous system is 1:4-6g / mL; the concentration of the ethanol-ammonium sulfate two-phase aqueous system is 20%-40% v / v, wherein the volume of ethanol to ammonium sulfate is 1:3-5; the ultrasonic extraction is performed at a power of 200-300W and a temperature of 40-50°C for 25-35min; the supercritical CO 2 The extraction pressure is 20-40MPa, the temperature is 35-45°C, the flow rate is 5-7mL / min, and the time is 1-3h.

4. An astaxanthin composition according to claim 1, It is characterized in that The preparation method of the white kidney bean extract comprises: adding white kidney bean powder into water, performing microwave extraction, collecting the extract, adding the residue into a 70%-80% v / v ethanol solution, performing ultrasonic extraction, collecting the extract, combining the two extracts, and vacuum concentrating to obtain the white kidney bean extract.

5. An astaxanthin composition according to claim 4, It is characterized in that The solid-liquid ratio of the white kidney bean powder to water is 1:8-10 g / mL; the microwave extraction is performed at 50-60°C and 400-600W for 30-60 min; the solid-liquid ratio of the residue to the ethanol solution is 1:4-6 g / mL; and the ultrasonic extraction is performed at 20-30 KHz and 100-200W for 40-60 min.

6. A method for preparing an astaxanthin composition according to claim 1, It is characterized in that The specific steps include: (1) mixing astaxanthin oil, lutein ester, DHA algae oil, soybean lecithin, wheat germ oil, and olive oil, stirring in a high-pressure homogenizer to obtain an oily composition, adding mono- and di-glycerol fatty acid esters to the oily composition, and stirring at high speed to obtain an oily emulsion; (2) adding the jujube seed powder, Aronia nigra fruit powder, white kidney bean extract, grape powder and vitamin C into water, and stirring with ultrasound to obtain an aqueous composition; (3) Mixing the aqueous composition and the oily emulsion, adding beeswax, stirring at a low speed, and adding mogroside to obtain the target astaxanthin composition.

7. A method for preparing an astaxanthin composition according to claim 6, It is characterized in that In step (1), the high-pressure homogenizer is: pressure 30-50MPa, time 5-10min; the high-speed shear stirring is stirring at 3000-4000rpm for 10-15min; In step (2), the amount of water added is 6-8 times the total weight of the wild jujube seed powder, black fruit glandular rib fruit powder, white kidney bean extract, grape powder and vitamin C; the ultrasonic stirring: power 30-50KHz, time 30-60min; In step (3), the low-speed stirring is at a speed of 50-150 rpm and a time of 1-3 h.

8. An astaxanthin capsule product, comprising the astaxanthin composition and a capsule shell.

9. An astaxanthin capsule product according to claim 8, It is characterized in that The capsule shell comprises the following raw materials in parts by weight: 1-3 parts gelatin, 3-7 parts glycerin, 0.01-0.03 parts caramel color, 0.05-0.08 parts xylitol.

10. A method for preparing an astaxanthin capsule product according to claim 8, It is characterized in that The specific steps include: (1) heating and stirring gelatin and xylitol at a temperature of 50-70° C. and a speed of 200-300 rpm for 30-40 minutes, adding glycerin, continuing to stir for 10-20 minutes, adding caramel color, stirring evenly, filtering, defoaming, and forming to obtain a capsule shell; (2) injecting the astaxanthin composition into a capsule shell to obtain the target astaxanthin capsule product.

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