Sunscreen cream containing recombinant X VII type humanized collagen and preparation method thereof

Abstract

The invention relates to the technical field of sunscreen cosmetics, and provides a sunscreen cream containing recombinant X VII type humanized collagen and a preparation method thereof. The sunscreen cream is prepared from the following components: a sunscreen additive, cetyl polyethylene glycol / polypropylene glycol-10 / 1 dimethyl siloxane, triethanolamine, polydimethylsiloxane, ethylhexyl palmitate, higher aliphatic alcohol, xanthan gum, a preservative and deionized water. Wherein the sunscreen additive is XVII type collagen / bacterial cellulose porous microsphere loaded ethylhexyl salicylate. The method can prevent ethylhexyl salicylate from being in direct contact with the skin, overcomes the defect that a chemical sun-screening agent is easy to irritate the skin, and can obviously improve the sun-screening effect by loading ethylhexyl salicylate in pores of the X VII type collagen / bacterial cellulose porous microspheres compared with direct addition.

Description

Technical Field

[0001] The invention relates to the technical field of sunscreen cosmetics, and provides a sunscreen containing recombinant type XVII humanized collagen and a preparation method thereof. Background Art

[0002] Excessive ultraviolet radiation will not only cause the skin to tan, affecting its appearance, but also cause photodamage to the skin and cause skin aging. Therefore, the application of sunscreen products is becoming more and more widespread, such as sunscreen clothing, sunscreen hats, sun umbrellas, and sunscreen cosmetics. Among them, sunscreen cosmetics are in the form of sunscreen lotions, sunscreen creams, sunscreen sprays, sunscreen gels, etc. They rely on the sunscreens they contain to prevent ultraviolet rays from causing harm to the human body. Since sunscreen cosmetics are used directly on human skin, their safety and mildness are highly valued.

[0003] Sunscreens are mainly divided into physical sunscreens and chemical sunscreens, and the sunscreen mechanisms of the two are different. Physical sunscreens mainly reduce ultraviolet radiation by changing the light path and physical blocking methods, through scattering and reflection. Chemical sunscreens mainly absorb ultraviolet rays and convert them into heat energy, thereby reducing the damage of ultraviolet rays to the skin. Chemical sunscreens are mainly aromatic compounds, and their structures contain chromophores with conjugated π electron systems.

[0004] As is known, chemical sunscreens can easily penetrate the skin and cause photosensitivity reactions, thus causing certain irritation to the skin, and in severe cases, causing inflammation, allergies and other side effects. Therefore, restrictions are imposed on the types and amounts of chemical sunscreens allowed to be added to sunscreen cosmetics. When the amount of chemical sunscreens added is low, the sunscreen effect needs to be improved. Summary of the invention

[0005] In view of the above situation, the present invention proposes a sunscreen containing recombinant type XVII humanized collagen and a preparation method, which can not only overcome the defect that chemical sunscreens are easy to irritate the skin, but also significantly improve the sunscreen effect.

[0006] To achieve the above purpose, the specific technical solution of the present invention is as follows:

[0007] The present invention first provides a sunscreen containing recombinant type XVII humanized collagen, wherein the components of the sunscreen include sunscreen additives, cetyl polyethylene glycol / polypropylene glycol-10 / 1 dimethylsiloxane, triethanolamine, polydimethylsiloxane, ethylhexyl palmitate, higher fatty alcohol, xanthan gum, preservatives, and deionized water.

[0008] Preferably, based on 100 parts by weight, the proportions of the components are: 5-15 parts of sunscreen additives, 3-5 parts of cetyl polyethylene glycol / polypropylene glycol-10 / 1 dimethicone, 1.5-2 parts of triethanolamine, 3-5 parts of polydimethicone, 3-5 parts of ethylhexyl palmitate, 0.2-0.5 parts of higher fatty alcohols, 0.1-0.3 parts of xanthan gum, 0.05-0.1 parts of preservatives, and the balance is deionized water.

[0009] Preferably, the higher fatty alcohol is one of myristyl alcohol, cetyl alcohol and stearyl alcohol.

[0010] Preferably, the preservative is one of methylparaben, ethylparaben and propylparaben.

[0011] The sunscreen additive is type XVII collagen / bacterial cellulose porous microspheres loaded with ethylhexyl salicylate.

[0012] Preferably, the sunscreen additive is prepared by vacuum adsorption method, and the specific preparation method is: add type XVII collagen / bacterial cellulose porous microspheres to a vacuum reactor, evacuate for 30-60 minutes, then add ethylhexyl salicylate, open the vent valve, stir at 50-100rpm for 20-30 minutes, and discharge. The principle is that when the porous microspheres are placed under vacuum conditions, negative pressure is formed inside the pores, and this negative pressure forms a pressure difference with the external positive pressure, which drives ethylhexyl salicylate to be absorbed into the pores of the porous microspheres. Further preferably, the mass ratio of type XVII collagen / bacterial cellulose porous microspheres to ethylhexyl salicylate is 4-5:1.

[0013] Preferably, the preparation method of type XVII collagen / bacterial cellulose porous microspheres is:

[0014] (1) adding bacterial cellulose to deionized water, homogenizing with a high-speed homogenizer, then transferring to a flask, adding sodium periodate, wrapping with tin foil, reacting at 45-55° C. for 8-12 hours, filtering with suction, adding the filter residue to an ethylene glycol solution and continuing to react for 50-70 minutes, filtering with suction, washing with deionized water, and freeze-drying to obtain 2,3-dialdehyde bacterial cellulose;

[0015] (2) mixing the 2,3-dialdehyde bacterial cellulose dispersion with the type XVII collagen gel solution, slowly stirring for 20-30 minutes, filtering at room temperature, washing with 40-50° C. hot water, and freeze-drying to obtain a type XVII collagen / bacterial cellulose composite material; the mass ratio of 2,3-dialdehyde bacterial cellulose to type XVII collagen is 1:3;

[0016] (3) Add the type XVII collagen / bacterial cellulose composite material to 1-ethyl-3-methylimidazole acetate, stir and dissolve at 85-90°C, then add hexadecane and an emulsifier, stir at 30-35°C for 4-5 hours to form an emulsion, then add n-butanol, stir for 2-3 hours, let stand to precipitate, filter, wash with acetone and deionized water, and freeze-dry to obtain type XVII collagen / bacterial cellulose porous microspheres.

[0017] In the preparation of the above-mentioned type XVII collagen / bacterial cellulose porous microspheres, preferably, in step (1), the dosage ratio of bacterial cellulose, deionized water, sodium periodate, and ethylene glycol is 1 g: 200-300 mL: 0.5-0.6 g: 0.3-0.35 g; further, the concentration of the ethylene glycol solution is 0.1-0.12 mol / L.

[0018] In the preparation of the above-mentioned type XVII collagen / bacterial cellulose porous microspheres, preferably, in step (2), the preparation method of the 2,3-dialdehyde bacterial cellulose dispersion is: add 2,3-dialdehyde bacterial cellulose into deionized water, stir evenly, and bathe in water at 40-50°C for 50-70 minutes; further, the content of 2,3-dialdehyde bacterial cellulose in the dispersion is 2-4 g / L.

[0019] In the preparation of the above-mentioned type XVII collagen / bacterial cellulose porous microspheres, preferably, in step (2), the method for preparing the type XVII collagen gel solution is: adding recombinant type XVII humanized collagen to deionized water, stirring evenly, and bathing at 40-50°C for 50-70 minutes; further, the content of recombinant type XVII humanized collagen in the gel solution is 8-10 g / L.

[0020] In the preparation of the above-mentioned type XVII collagen / bacterial cellulose porous microspheres, preferably, in step (3), the dosage ratio of type XVII collagen / bacterial cellulose composite material, 1-ethyl-3-methylimidazole acetate, hexadecane, emulsifier, and n-butanol is 3-5g:100g:800-1000g:16-20g:0.8-1.2L; further, the emulsifier is composed of Tween 80 and Span 80 in a mass ratio of 1:1.

[0021] It is well known that bacterial cellulose is formed by nano-microfibers entangled with each other and has a three-dimensional network structure. The present invention uses bacterial cellulose and type XVII collagen to prepare composite porous microspheres. First, sodium periodate is used as an oxidant to selectively oxidize the hydroxyl groups of bacterial cellulose, and the hydroxyl groups at the C2 and C3 positions are oxidized to aldehyde groups. Then, the aldehyde groups are used to react with the amino groups in type XVII collagen to obtain a type XVII collagen / bacterial cellulose composite material. Finally, the composite material is prepared into type XVII collagen / bacterial cellulose porous microspheres by an inverse suspension regeneration method. Furthermore, the present invention uses a vacuum adsorption method to adsorb ethylhexyl salicylate into the pores of type XVII collagen / bacterial cellulose porous microspheres to prepare a sunscreen additive.

[0022] The present invention can prevent ethylhexyl salicylate from directly contacting the skin by loading ethylhexyl salicylate into the pores of the porous microspheres, thereby preventing it from penetrating the skin or causing adverse reactions. Bacterial cellulose and collagen contact the skin, and both have good biocompatibility and good affinity to the skin. Compared with proteins containing more aromatic amino acids, the structure of the type XVII collagen used in the present invention is mainly non-aromatic amino acids, and does not have the conjugation effect similar to aromatic amino acids, so its ultraviolet light reaction is weaker and it is more gentle to the skin.

[0023] The present invention uses type XVII collagen / bacterial cellulose porous microspheres loaded with ethylhexyl salicylate as a sunscreen additive. Under ultraviolet irradiation, the surface of the porous microspheres can reflect and scatter part of the ultraviolet rays, and the ultraviolet rays that are not reflected and scattered by the surface enter the interior of the microspheres. This part of the ultraviolet rays is continuously attenuated by repeated reflections of the pore structure and is absorbed and weakened by ethylhexyl salicylate. Therefore, under the dual effects of physical attenuation and chemical absorption, a good sunscreen effect can be achieved.

[0024] Furthermore, the structure of type XVII collagen contains amide groups, and Schiff base structures are also generated when preparing type XVII collagen / bacterial cellulose porous microspheres. Both the amide groups and the Schiff base structures can form hydrogen bonds with the hydroxyl groups of ethylhexyl salicylate. On the one hand, the formation of hydrogen bonds can improve the interface binding between ethylhexyl salicylate and type XVII collagen / bacterial cellulose porous microspheres, improve the loading firmness of ethylhexyl salicylate, and reduce its leakage tendency, so as to better prevent it from directly contacting the skin. On the other hand, C=O (from amide) and C=N (from Schiff base) can produce n→π* transitions under ultraviolet irradiation. When the amide groups and Schiff base structures at the pore walls inside the porous microspheres form hydrogen bonds with the hydroxyl groups of ethylhexyl salicylate, the delocalization range of the conjugated electrons of ethylhexyl salicylate can be increased, thereby improving its ultraviolet absorption effect.

[0025] The present invention also provides a method for preparing the sunscreen containing the recombinant type XVII humanized collagen. The method comprises the following steps:

[0026] (1) mixing triethanolamine, xanthan gum and deionized water at 65-75° C. to obtain phase A;

[0027] (2) mixing the sunscreen additive, cetyl polyethylene glycol / polypropylene glycol-10 / 1 dimethicone, polydimethylsiloxane, ethylhexyl palmitate, higher fatty alcohol, and preservative at 65-75° C. to obtain phase B;

[0028] (3) While stirring, add phase A to phase B and mix well at 40-50° C. to obtain a sunscreen.

[0029] In summary, the present invention provides a sunscreen containing recombinant type XVII humanized collagen and a preparation method thereof. Compared with the prior art, the present invention has the following outstanding features and excellent effects:

[0030] First, the present invention loads ethylhexyl salicylate into the pores of type XVII collagen / bacterial cellulose porous microspheres, preventing ethylhexyl salicylate from directly contacting the skin, but bacterial cellulose and collagen contact the skin, thus overcoming the defect that chemical sunscreens easily irritate the skin. In addition, hydrogen bonds are formed between the amide groups and Schiff base structures of the porous microspheres and the hydroxyl groups of ethylhexyl salicylate, thereby improving the loading firmness of ethylhexyl salicylate, reducing its leakage tendency, and better preventing it from directly contacting the skin.

[0031] Second, when the sunscreen additive of the present invention is irradiated with ultraviolet rays, part of the ultraviolet rays are reflected and scattered by the surface of the porous microspheres, and part of the ultraviolet rays enter the microspheres, are repeatedly reflected by the pore structure inside the microspheres, and are absorbed by ethylhexyl salicylate, thereby achieving a good sunscreen effect. In addition, the amide group and Schiff base structure of the porous microspheres form hydrogen bonds with the hydroxyl group of ethylhexyl salicylate, which can increase the delocalization range of the conjugated electrons of ethylhexyl salicylate, thereby improving its ultraviolet absorption effect. DETAILED DESCRIPTION

[0032] The present invention is further described in detail below through specific embodiments, but this should not be construed as limiting the scope of the present invention to the following examples. Without departing from the above-mentioned method concept of the present invention, various substitutions or changes made according to common technical knowledge and conventional means in the art should be included in the scope of the present invention.

[0033] Example 1

[0034] Preparation of sunscreen additive type ⅩⅦ collagen / bacterial cellulose porous microspheres loaded with ethylhexyl salicylate:

[0035] (1) Add bacterial cellulose to deionized water, homogenize with a high-speed homogenizer, then transfer to a flask, add sodium periodate, wrap with tin foil, react at 50°C for 10 hours, filter, add the filter residue to ethylene glycol solution and continue to react for 60 minutes, filter, wash with deionized water, freeze-dry, and obtain 2,3-dialdehyde bacterial cellulose; the amount ratio of bacterial cellulose, deionized water, sodium periodate, and ethylene glycol is 1g:250mL:0.55g:0.3g; the concentration of ethylene glycol solution is 0.1mol / L;

[0036] (2) Add 2,3-dialdehyde bacterial cellulose to deionized water, stir evenly, and bathe at 45°C for 60 minutes to obtain a 2,3-dialdehyde bacterial cellulose dispersion (3 g / L); add recombinant type XVII humanized collagen to deionized water, stir evenly, and bathe at 45°C for 60 minutes to obtain a type XVII collagen gel solution (9 g / L); mix the two in equal volumes, stir slowly for 25 minutes, filter at room temperature, wash with hot water at 45°C, and freeze-dry to obtain a type XVII collagen / bacterial cellulose composite material;

[0037] (3) Adding the type XVII collagen / bacterial cellulose composite material to 1-ethyl-3-methylimidazole acetate, stirring and dissolving at 90°C, then adding hexadecane and an emulsifier, stirring at 30°C for 4.5 hours to form an emulsion, then adding n-butanol, stirring for 2.5 hours, standing for precipitation, filtering, washing with acetone and deionized water, and freeze-drying to obtain type XVII collagen / bacterial cellulose porous microspheres; the amount ratio of type XVII collagen / bacterial cellulose composite material, 1-ethyl-3-methylimidazole acetate, hexadecane, Tween 80, Span 80, and n-butanol is 4g:100g:900g:9g:9g:1L;

[0038] (4) Add type XVII collagen / bacterial cellulose porous microspheres into a vacuum reactor, evacuate for 60 minutes, then add ethylhexyl salicylate, open the vent valve, stir at 60 rpm for 25 minutes, and discharge to obtain type XVII collagen / bacterial cellulose porous microspheres loaded with ethylhexyl salicylate; the mass ratio of type XVII collagen / bacterial cellulose porous microspheres to ethylhexyl salicylate is 5:1.

[0039] Example 2

[0040] Preparation of sunscreen additive type ⅩⅦ collagen / bacterial cellulose porous microspheres loaded with ethylhexyl salicylate:

[0041] In step (4), the mass ratio of type XVII collagen / bacterial cellulose porous microspheres to ethylhexyl salicylate is 4.5:1, and other preparation conditions are consistent with those in Example 1.

[0042] Example 3

[0043] Preparation of sunscreen additive type ⅩⅦ collagen / bacterial cellulose porous microspheres loaded with ethylhexyl salicylate:

[0044] In step (4), the mass ratio of type XVII collagen / bacterial cellulose porous microspheres to ethylhexyl salicylate is 4:1, and other preparation conditions are consistent with those in Example 1.

[0045] Example 4

[0046] The sunscreen cream was prepared using the sunscreen additive of Example 1:

[0047] (1) Mix triethanolamine, xanthan gum and deionized water at 70° C. to obtain phase A; (2) Mix sunscreen additives, cetyl polyethylene glycol / polypropylene glycol-10 / 1 dimethicone, polydimethylsiloxane, ethylhexyl palmitate, cetyl alcohol and propylparaben at 70° C. to obtain phase B; (3) Add phase A to phase B under stirring and mix at 45° C. to obtain a sunscreen.

[0048] Based on 100 parts by weight, the distribution ratio of each component is: 5 parts of sunscreen additives, 4 parts of cetyl polyethylene glycol / polypropylene glycol-10 / 1 dimethicone, 1.8 parts of triethanolamine, 4 parts of polydimethicone, 4 parts of ethylhexyl palmitate, 0.4 parts of cetyl alcohol, 0.2 parts of xanthan gum, 0.1 parts of propylparaben, and the balance is deionized water.

[0049] Example 5

[0050] The sunscreen cream was prepared using the sunscreen additive of Example 1:

[0051] (1) Mix triethanolamine, xanthan gum and deionized water at 70° C. to obtain phase A; (2) Mix sunscreen additives, cetyl polyethylene glycol / polypropylene glycol-10 / 1 dimethicone, polydimethylsiloxane, ethylhexyl palmitate, cetyl alcohol and propylparaben at 70° C. to obtain phase B; (3) Add phase A to phase B under stirring and mix at 45° C. to obtain a sunscreen.

[0052] Based on 100 parts by weight, the distribution ratio of each component is: 10 parts of sunscreen additives, 4 parts of cetyl polyethylene glycol / polypropylene glycol-10 / 1 dimethicone, 1.8 parts of triethanolamine, 4 parts of polydimethicone, 4 parts of ethylhexyl palmitate, 0.4 parts of cetyl alcohol, 0.2 parts of xanthan gum, 0.1 parts of propylparaben, and the balance is deionized water.

[0053] Example 6

[0054] The sunscreen cream was prepared using the sunscreen additive of Example 1:

[0055] (1) Mix triethanolamine, xanthan gum and deionized water at 70° C. to obtain phase A; (2) Mix sunscreen additives, cetyl polyethylene glycol / polypropylene glycol-10 / 1 dimethicone, polydimethylsiloxane, ethylhexyl palmitate, cetyl alcohol and propylparaben at 70° C. to obtain phase B; (3) Add phase A to phase B under stirring and mix at 45° C. to obtain a sunscreen.

[0056] Based on 100 parts by weight, the distribution ratio of each component is: 15 parts of sunscreen additives, 4 parts of cetyl polyethylene glycol / polypropylene glycol-10 / 1 dimethicone, 1.8 parts of triethanolamine, 4 parts of polydimethicone, 4 parts of ethylhexyl palmitate, 0.4 parts of cetyl alcohol, 0.2 parts of xanthan gum, 0.1 parts of propylparaben, and the balance is deionized water.

[0057] Comparative Example 1

[0058] Prepare the sunscreen:

[0059] Ethylhexyl salicylate was not loaded into the pores of type XVII collagen / bacterial cellulose porous microspheres. Instead, type XVII collagen / bacterial cellulose porous microspheres and ethylhexyl salicylate were directly added to the cosmetic components (added to phase B). Other preparation conditions were consistent with those in Example 6.

[0060] Comparative Example 2

[0061] Prepare the sunscreen:

[0062] Bacterial cellulose and recombinant type XVII humanized collagen were not prepared into porous microspheres, and ethylhexyl salicylate was not loaded. Instead, bacterial cellulose, recombinant type XVII humanized collagen, and ethylhexyl salicylate were directly added to the cosmetic components (added to phase B), and other preparation conditions were consistent with Example 6.

[0063] Among the raw materials involved in the above preparation process, bacterial cellulose was purchased from Nanjing Tianlu Nanotechnology Co., Ltd., recombinant type XVII humanized collagen was purchased from Shanghai Zeye Biotechnology Co., Ltd., and the rest were common commercially available products.

[0064] Performance Test:

[0065] (1) Weigh 1 g of each sunscreen additive prepared in Examples 1-3, add 1.5 L of deionized water, stir for 24 h in dark at room temperature (stirring speed is 500 rpm), and then centrifuge for 20 min (centrifugal speed is 10000 rpm). Filter the centrifuged clear liquid with a 0.22 μm organic filter head, take 5 mL of the filtrate, make up to volume with anhydrous ethanol in a 25 mL brown volumetric flask, and measure λ with a UV-visible spectrophotometer. max= UV absorbance value at 306nm, and compare with the standard curve of ethylhexyl salicylate to get the corresponding concentration, and then get the mass of ethylhexyl salicylate in the centrifuged clear liquid. Calculate the leakage rate of ethylhexyl salicylate according to the following formula: Leakage rate = m 1 ×100% / m 0 , where m 1 is the leakage mass (the mass of ethylhexyl salicylate in the centrifuged clear liquid), m 0 is the mass of ethylhexyl salicylate loaded in the sunscreen additive. The results are shown in Table 1. It can be seen that the leakage rates of ethylhexyl salicylate in Examples 1-3 are all very low, indicating that the method of the present invention can firmly load ethylhexyl salicylate in the pores of type XVII collagen / bacterial cellulose porous microspheres to prevent it from directly contacting the skin.

[0066] Table 1:

[0067] Performance Indicators Example 1 Example 2 Example 3 Leakage rate (%) 2.15 2.22 2.38

[0068] (2) First, prepare a base cream (the ratio is: 4 parts of cetyl polyethylene glycol / polypropylene glycol-10 / 1 dimethicone, 1.8 parts of triethanolamine, 4 parts of polydimethylsiloxane, 4 parts of ethylhexyl palmitate, 0.4 parts of cetyl alcohol, 0.2 parts of xanthan gum, 0.1 parts of propylparaben, and deionized water to 100 parts); test the SPF value with reference to the 2015 edition of the "Technical Specification for Safety of Cosmetics", and evenly spot the base cream, the sunscreen of Examples 4-6, and the sunscreen of Comparative Examples 1-2 on a 3M Transpore™ tape (2 mg / cm 2 ), wear a disposable latex finger cot, apply the sample evenly, keep it away from light for 15 minutes, and use an SPF-290 sun protection factor tester to measure the SPF value. Calculate the improvement rate of the sun protection effect of the test sample relative to the base cream according to the following formula: Improvement rate = (SPF 1 -SPF 0 )×100% / SPF 0 , among which SPF 1 is the SPF value of the test sample (sunscreen of Examples 4-6 or Comparative Examples 1-2), SPF 0is the SPF value of the base cream. The results are shown in Table 2. It can be seen that: first, in Examples 4-6, as the amount of sunscreen additives increases, the sunscreen effect is better; second, in Comparative Example 1, ethylhexyl salicylate is not loaded into the pores of the porous microspheres of type XVII collagen / bacterial cellulose, and it is difficult to increase the conjugated electron delocalization range of ethylhexyl salicylate through the amide group and Schiff base structure of the porous microspheres and the hydroxyl group of ethylhexyl salicylate to form hydrogen bonds, so the sunscreen effect is worse than that of Example 6; third, in Comparative Example 2, bacterial cellulose and recombinant type XVII humanized collagen are not prepared into porous microspheres, and the physical attenuation effect of the porous microspheres on ultraviolet rays cannot be exerted, so the sunscreen effect is worse than that of Comparative Example 1.

[0069] Table 2:

[0070] Performance Indicators Example 4 Example 5 Example 6 Comparative Example 1 Comparative Example 2 Improvement rate (%) 39.1 59.8 72.3 57.7 41.2

[0071] (3) 150 healthy female volunteers were selected and randomly divided into 3 groups, each with 50 volunteers. The volunteers were aged between 20 and 30 years old, had no skin abnormalities, were not pregnant or lactating, and had not participated in other tests or treatments in the past three months. The 3 groups of volunteers used the sunscreens of Example 6, Comparative Example 1, and Comparative Example 2 respectively. The use time was every morning after daily cleansing and skin care for 4 consecutive weeks. Smoking and drinking were prohibited during the use period, and the cleansing and skin care products used were the products that had been used for a long time before. If there were adverse reactions during the test, the use of sunscreen should be stopped immediately. A questionnaire survey was conducted on the volunteers, and the number of people in grade A, grade B, and grade C was counted. Grade A means that there was no redness, itching or rash at all, grade B means that there was slight redness, itching or rash, and grade C means that there was obvious redness, itching or rash. The results are shown in Table 3. It can be seen that the present invention significantly reduces skin irritation by loading ethylhexyl salicylate into the pores of type XVII collagen / bacterial cellulose porous microspheres.

[0072] Table 3:

[0073] Count the number of people Example 6 Comparative Example 1 Comparative Example 2 A-level 42 18 15 Class B 8 28 30 C-Class 0 4 5

Claims

1. A sunscreen containing recombinant type XVII humanized collagen, the components of which include sunscreen additives, cetyl polyethylene glycol / polypropylene glycol-10 / 1 dimethicone, triethanolamine, polydimethylsiloxane, ethylhexyl palmitate, higher fatty alcohol, xanthan gum, preservatives, and deionized water, characterized in that: The sunscreen additive is type XVII collagen / bacterial cellulose porous microspheres loaded with ethylhexyl salicylate.

2. A sunscreen containing recombinant type XVII humanized collagen according to claim 1, characterized in that: Based on 100 parts by weight, the distribution ratio of each component is: 5-15 parts of sunscreen additives, 3-5 parts of cetyl polyethylene glycol / polypropylene glycol-10 / 1 dimethicone, 1.5-2 parts of triethanolamine, 3-5 parts of polydimethicone, 3-5 parts of ethylhexyl palmitate, 0.2-0.5 parts of higher fatty alcohols, 0.1-0.3 parts of xanthan gum, 0.05-0.1 parts of preservatives, and the balance is deionized water.

3. A sunscreen containing recombinant type XVII humanized collagen according to claim 1, characterized in that: The higher fatty alcohol is one of myristyl alcohol, cetyl alcohol and stearyl alcohol; the preservative is one of methylparaben, ethylparaben and propylparaben.

4. A sunscreen containing recombinant type XVII humanized collagen according to claim 1, characterized in that: The preparation method of the sunscreen additive is as follows: add type XVII collagen / bacterial cellulose porous microspheres into a vacuum reactor, evacuate for 30-60 minutes, add ethylhexyl salicylate, open the vent valve, stir at 50-100 rpm for 20-30 minutes, and discharge.

5. A sunscreen containing recombinant type XVII humanized collagen according to claim 4, characterized in that: The mass ratio of type ⅩⅦ collagen / bacterial cellulose porous microspheres and ethylhexyl salicylate is 4-5:

1.

6. A sunscreen containing recombinant type XVII humanized collagen according to claim 1, characterized in that: The preparation method of type ⅩⅦ collagen / bacterial cellulose porous microspheres is as follows: (1) adding bacterial cellulose to deionized water, homogenizing with a high-speed homogenizer, then transferring to a flask, adding sodium periodate, wrapping with tin foil, reacting at 45-55° C. for 8-12 hours, filtering with suction, adding the filter residue to an ethylene glycol solution and continuing to react for 50-70 minutes, filtering with suction, washing with deionized water, and freeze-drying to obtain 2,3-dialdehyde bacterial cellulose; (2) mixing the 2,3-dialdehyde bacterial cellulose dispersion with the type XVII collagen gel solution, slowly stirring for 20-30 minutes, filtering at room temperature, washing with 40-50° C. hot water, and freeze-drying to obtain a type XVII collagen / bacterial cellulose composite material; the mass ratio of 2,3-dialdehyde bacterial cellulose to type XVII collagen is 1:3; (3) Add the type XVII collagen / bacterial cellulose composite material to 1-ethyl-3-methylimidazole acetate, stir and dissolve at 85-90°C, then add hexadecane and an emulsifier, stir at 30-35°C for 4-5 hours to form an emulsion, then add n-butanol, stir for 2-3 hours, let stand to precipitate, filter, wash with acetone and deionized water, and freeze-dry to obtain type XVII collagen / bacterial cellulose porous microspheres.

7. A sunscreen containing recombinant type XVII humanized collagen according to claim 6, characterized in that: In step (1), the usage ratio of bacterial cellulose, deionized water, sodium periodate and ethylene glycol is 1 g: 200-300 mL: 0.5-0.6 g: 0.3-0.35 g; and the concentration of the ethylene glycol solution is 0.1-0.12 mol / L.

8. A sunscreen containing recombinant type XVII humanized collagen according to claim 6, characterized in that: In step (2), the preparation method of the 2,3-dialdehyde bacterial cellulose dispersion is as follows: adding 2,3-dialdehyde bacterial cellulose to deionized water, stirring evenly, and bathing at 40-50° C. for 50-70 minutes; the content of 2,3-dialdehyde bacterial cellulose in the dispersion is 2-4 g / L; In step (2), the preparation method of type XVII collagen gel solution is: add recombinant type XVII humanized collagen to deionized water, stir evenly, and bathe at 40-50°C for 50-70 minutes; the content of recombinant type XVII humanized collagen in the gel solution is 8-10 g / L.

9. A sunscreen containing recombinant type XVII humanized collagen according to claim 6, characterized in that: In step (3), the dosage ratio of type XVII collagen / bacterial cellulose composite material, 1-ethyl-3-methylimidazole acetate, hexadecane, emulsifier and n-butanol is 3-5g:100g:800-1000g:16-20g:0.8-1.2L; the emulsifier is composed of Tween 80 and Span 80 in a mass ratio of 1:

1.

10. A method for preparing a sunscreen containing recombinant type XVII humanized collagen according to any one of claims 1 to 9, characterized in that: The method comprises the following preparation steps: (1) mixing triethanolamine, xanthan gum and deionized water at 65-75° C. to obtain phase A; (2) mixing the sunscreen additive, cetyl polyethylene glycol / polypropylene glycol-10 / 1 dimethicone, polydimethylsiloxane, ethylhexyl palmitate, higher fatty alcohol, and preservative at 65-75° C. to obtain phase B; (3) While stirring, add phase A to phase B and mix well at 40-50° C. to obtain a sunscreen.