Veterinary traditional Chinese medicine oral liquid for promoting growth of broiler chickens and application thereof
By using traditional Chinese medicine oral liquid prepared by traditional Chinese medicine raw materials and extraction technology, the drug residues and drug resistance problems caused by traditional veterinary drugs are solved, the growth rate and health level of broilers are improved, and safe and effective broiler breeding is achieved.
Patent Information
- Application Number
- CN202411395828.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-10-08
- Publication Date
- 2024-12-13
AI Technical Summary
Traditional chemical synthetic veterinary drugs cause drug residues and drug resistance in broiler breeding, affecting the growth rate and health level of broiler chickens, and do not meet the requirements of food safety and green breeding.
A veterinary Chinese medicine oral liquid is used, with raw materials including chili peppers, mushroom stalks, hawthorn, fried malt, fried yam, atractylodes and roasted licorice. The effective ingredients are extracted through the collaborative extraction technology of ethanol and glycerol, combined with water decoction and reduced pressure concentration. , after adding auxiliary materials, the finished product is prepared to promote the growth of broiler chickens.
It improves the growth rate and health level of broiler chickens, reduces drug residue risks, enhances growth performance and product quality, and complies with green breeding standards.
Smart Images
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Abstract
Description
Technical Field
[0001] The present invention relates to the technical field of Chinese medicine preparations, and in particular to a veterinary Chinese medicine oral liquid for promoting the growth of broilers and an application thereof. Background Art
[0002] In modern poultry farming, improving the growth rate and health level of broilers has always been the focus of farmers and related industries. As people's requirements for food safety and green farming continue to increase, traditional chemical synthetic veterinary drugs have gradually exposed some problems during use, such as drug residues and drug resistance, which not only pose a potential threat to consumers' health, but also bring challenges to the sustainable development of the farming industry.
[0003] As a traditional Chinese medicine treasure, Chinese medicine has the advantages of being natural, safe, and having few side effects. In the field of veterinary medicine, the application of Chinese medicine is also gaining more and more attention. Many Chinese medicines have the functions of regulating the body's immune function, promoting growth and development, and having antibacterial and antiviral effects.
[0004] Traditional farming models often rely on a large number of antibiotics and chemical drugs to prevent and treat diseases, which not only easily leads to the development of drug resistance, but also affects the growth performance and product quality of broilers. Therefore, a veterinary Chinese medicine oral liquid that promotes the growth of broilers provides farmers with a safer and more effective option, and is expected to play an important role in the field of broiler farming. Summary of the invention
[0005] The invention provides a veterinary Chinese medicine oral liquid for promoting the growth of broiler chickens and application thereof, which solves the problem of slow growth of broiler chickens in the related art.
[0006] The technical scheme of the present invention is as follows: The present invention proposes a veterinary Chinese medicine oral liquid for promoting the growth of broilers, and the raw materials include the following components in parts by weight: 10-30 parts of peppers, 60-80 parts of mushroom stems, 20-40 parts of hawthorn, 30-50 parts of fried malt, 20-40 parts of fried yam, 20-40 parts of white atractylodes, and 10-30 parts of roasted licorice.
[0007] As a further technical solution, the raw materials include the following components in parts by weight: 15-25 parts of chili peppers, 70-80 parts of mushroom stems, 30-40 parts of hawthorn, 35-45 parts of fried malt, 25-23 parts of fried yam, 25-30 parts of Atractylodes macrocephala, and 10-20 parts of roasted licorice.
[0008] The present invention also provides a method for preparing a veterinary Chinese medicine oral liquid for promoting the growth of broiler chickens, comprising the following steps: S1, adding pepper to an extractant for extraction, filtering to obtain a residue and a filtrate, and concentrating the filtrate under reduced pressure to obtain an extract; S2, soaking the remaining raw materials in water, adding the medicinal residue, decoction by water extraction to obtain a decoction, and concentrating under reduced pressure and filtering to obtain a clear liquid; S3, after mixing the extract with the clear liquid, adding auxiliary materials to prepare the liquid, packaging and sterilizing to obtain a finished veterinary Chinese medicine oral liquid; The auxiliary materials include 0.01 g / mL calcium propionate, 0.0003 g / mL Tween 80, and 0.0001 g / mL PEG400.
[0009] As a further technical solution, the extraction of the pepper is carried out in two steps, firstly alcohol extraction and then glycerol extraction.
[0010] As a further technical solution, the glycerol concentration used in the glycerol extraction is 55wt%~65wt%.
[0011] In the present invention, ethanol and glycerol are used to extract pepper in two steps, and the synergistic effect of the two extracts is brought into play, so that the effective ingredients in the pepper are extracted more comprehensively, the types and contents of active substances in the oral liquid are increased, the extraction efficiency is improved, and the growth-promoting effect of the veterinary Chinese medicine oral liquid is enhanced.
[0012] As a further technical solution, the ethanol concentration used in the alcohol extraction is 80wt%, the temperature of the alcohol extraction is 33-37°C, and the time is 5.5-6.5h.
[0013] As a further technical solution, the extractant during the alcohol extraction also includes soybean oil, and the mass ratio of the ethanol to the soybean oil is 5:1-3.
[0014] As a further technical solution, the water decoction method is carried out twice, the first water decoction time is 1.5-2.5 hours, and the second water decoction time is 45-75 minutes.
[0015] As a further technical solution, the concentrated drug content of the decoction is 1 g / mL.
[0016] The present invention also proposes the use of the veterinary Chinese medicine oral liquid for promoting broiler growth or the veterinary Chinese medicine oral liquid for promoting broiler growth prepared by the preparation method in the field of broiler breeding.
[0017] The working principle and beneficial effects of the present invention are: In the present invention, different concentrations of glycerol are used to extract pepper, so as to improve the extraction rate of active ingredients of pepper, enhance the antioxidant effect of active ingredients of pepper, maintain the stability of pepper extract, increase the solubility of pepper extract, and improve the growth-promoting effect of veterinary Chinese medicine oral liquid. DETAILED DESCRIPTION
[0018] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.
[0019] In the following examples and comparative examples: Glycerol: concentration 80wt%, specific gravity 1.25, pH 6~7, manufacturer is Shandong Luliya New Materials Co., Ltd.; The following other concentrations of glycerol are prepared by adding water to 80wt% glycerol.
[0020] Example 1 S1. Cut 15 parts of pepper into pieces of about 1 cm with a chopper, add them to 105 parts of ethanol for extraction, extract at 33°C for 6.5h, stir for 5min every 0.5h, filter to obtain alcohol-extracted drug residues and filtrate I, add 105 parts of glycerol (concentration of 50wt%) to extract at 40°C for 5h, filter to obtain glycerol-extracted drug residues and filtrate II, concentrate filtrate I and filtrate II under reduced pressure, add 0.05g / mL phosphoric acid solution, adjust pH to about 3.5, and obtain extract; S2, weigh 70 parts of mushroom stems, 30 parts of hawthorn, 35 parts of fried malt, 25 parts of fried yam, 25 parts of Atractylodes macrocephala, and 10 parts of roasted licorice, put them into a multifunctional extraction tank, add 1560 parts of water and soak for 3.5 hours, add glycerin to extract the residue, decoct for 1.5 hours, filter to obtain the residue and decoction, add 1170 parts of water to the residue again, decoct for 45 minutes, filter to obtain the residue and decoction, decoct twice, decompress and concentrate, refrigerate and stand for 12 hours, concentrate to a drug content of 1g / mL, filter with a 500-mesh vibration sieve to obtain a clear liquid; S3. After mixing the extract with the clear liquid, add 0.01g / mL of calcium propionate, 0.0003g / mL of Tween 80 and 0.0001g / mL of PEG400 to prepare the solution, adjust the pH value to meet the internal control standard, add water to dissolve to 1g / mL of crude drug, stir evenly, and obtain the finished product of veterinary Chinese medicine oral liquid after packaging and sterilization.
[0021] Example 2 S1, cut 20 parts of pepper into pieces of about 1 cm with a chopper, add 140 parts of ethanol to extract, extract at 35 ° C for 6 hours, stir for 5 minutes every 0.5 hours, filter to obtain alcohol-extracted drug residues and filtrate I, add 140 parts of glycerol (concentration of 50wt%) to extract at 40 ° C for 5 hours, filter to obtain glycerol-extracted drug residues and filtrate II, concentrate filtrate I and filtrate II under reduced pressure, add 0.05g / mL phosphoric acid solution, adjust pH to about 3.5 to obtain extract; S2, weigh 75 parts of mushroom stems, 35 parts of hawthorn, 40 parts of fried malt, 30 parts of fried yam, 28 parts of Atractylodes macrocephala, and 16 parts of roasted licorice, put them into a multifunctional extraction tank, add 1792 parts of water and soak for 4 hours, add glycerin to extract the residue, decoct for 2 hours, filter to obtain the residue and decoction, add 1344 parts of water to the residue again, decoct for 60 minutes, filter to obtain the residue and decoction, decoct and concentrate the decoction twice under reduced pressure, refrigerate and stand for 12 hours, concentrate to a drug content of 1g / mL, filter with a 500-mesh vibration sieve to obtain a clear liquid; S3. After mixing the extract with the clear liquid, add 0.01g / mL of calcium propionate, 0.0003g / mL of Tween 80 and 0.0001g / mL of PEG400 to prepare the solution, adjust the pH value to meet the internal control standard, add water to dissolve to 1g / mL of crude drug, stir evenly, and obtain the finished product of veterinary Chinese medicine oral liquid after packaging and sterilization.
[0022] Example 3 S1, cut 25 parts of pepper into pieces of about 1 cm with a chopper, add 175 parts of ethanol to extract, extract at 37 ° C for 5.5 hours, stir for 5 minutes every 0.5 hours, filter to obtain alcohol-extracted drug residues and filtrate I, add 175 parts of glycerol (concentration of 50wt%) to extract at 40 ° C for 5 hours, filter to obtain glycerol-extracted drug residues and filtrate II, concentrate filtrate I and filtrate II under reduced pressure, add 0.05g / mL phosphoric acid solution, adjust pH to about 3.5 to obtain extract; S2, weigh 80 parts of shiitake mushroom stems, 40 parts of hawthorn, 45 parts of fried malt, 35 parts of fried yam, 30 parts of Atractylodes macrocephala, and 20 parts of roasted licorice, put them into a multifunctional extraction tank, add 2000 parts of water and soak for 4.5 hours, add glycerin to extract the residue, decoct for 2.5 hours, filter to obtain the residue and decoction, add 1500 parts of water to the residue again, decoct for 75 minutes, filter to obtain the residue and decoction, decoct the decoction twice under reduced pressure, refrigerate and stand for 12 hours, concentrate to a drug content of 1g / mL, filter with a 500-mesh vibration sieve to obtain a clear liquid; S3. After mixing the extract with the clear liquid, add 0.01g / mL of calcium propionate, 0.0003g / mL of Tween 80 and 0.0001g / mL of PEG400 to prepare the solution, adjust the pH value to meet the internal control standard, add water to dissolve to 1g / mL of crude drug, stir evenly, and obtain the finished product of veterinary Chinese medicine oral liquid after packaging and sterilization.
[0023] Example 4 Compared with Example 2, the difference in Example 4 is that the concentration of added glycerol is 55 wt %.
[0024] Example 5 Compared with Example 2, the difference in Example 5 is that the concentration of added glycerol is 60wt%.
[0025] Example 6 Compared with Example 2, the difference in Example 6 is that the concentration of added glycerol is 65wt%.
[0026] Example 7 Compared with Example 2, the difference in Example 7 is that the concentration of added glycerol is 70wt%.
[0027] Example 8 Compared with Example 5, the difference in Example 8 is that 140 parts of ethanol are replaced by 120 parts of ethanol and 20 parts of soybean oil.
[0028] Example 9 Compared with Example 5, Example 9 is different in that 140 parts of ethanol are replaced by 100 parts of ethanol and 40 parts of soybean oil.
[0029] Example 10 Compared with Example 5, Example 10 is different in that 140 parts of ethanol are replaced by 87.5 parts of ethanol and 52.5 parts of soybean oil.
[0030] Embodiment 11 Compared with Example 5, Example 11 is different in that 140 parts of ethanol are replaced by 70 parts of ethanol and 70 parts of soybean oil.
[0031] Comparative Example 1 Compared with Example 2, the difference of Comparative Example 1 is that the extract is prepared by the following steps: Cut 20 parts of pepper into pieces of about 1 cm with a chopper, add them to 140 parts of ethanol for extraction, extract at 35°C for 6 hours, stir for 5 minutes every 0.5 hours, filter to obtain alcohol-extracted medicinal residues and filtrate, concentrate the filtrate under reduced pressure, add 0.05 g / mL phosphoric acid solution, adjust the pH value to about 3.5 to obtain an extract.
[0032] Comparative Example 2 Compared with Example 2, the difference of Comparative Example 2 is that the extract is prepared by the following steps: Cut 20 parts of pepper into pieces of about 1 cm with a chopper, add them to 140 parts of glycerol (concentration of 60wt%), extract at 40℃ for 5 hours, filter to obtain glycerin-extracted residue and filtrate II, add 140 parts of ethanol to the glycerin-extracted residue, extract at 35℃ for 6 hours, stir for 5 minutes every 0.5 hours, filter to obtain alcohol-extracted residue and filtrate II, concentrate filtrate I and filtrate II under reduced pressure, add 0.05g / mL phosphoric acid solution, adjust the pH value to about 3.5 to obtain an extract.
[0033] Test 1 High performance liquid chromatography was used to detect the capsaicin content in the veterinary Chinese medicine oral liquids prepared in Examples 1 to 11 and Comparative Examples 1 to 2. The detection method refers to the 2020 edition of the Chinese Pharmacopoeia "Pharmacopoeia of the People's Republic of China".
[0034] The test results are shown in Table 1 below: Table 1 Capsaicin content in the veterinary Chinese medicine oral solution of Examples 1 to 11 and Comparative Examples 1 to 2
[0035] Compared with Comparative Example 1, Example 2 adds a glycerol extraction step after the pepper alcohol extraction. As a result, the capsaicin content of Example 2 is better than that of Comparative Example 1, indicating that adding the glycerol extraction step can improve the extraction of effective ingredients of pepper. In Examples 2 and Examples 4 to 7, different concentrations of glycerol were added to extract the pepper. As a result, the capsaicin content of Examples 4 to 6 was better than that of Examples 2 and 7, indicating that when the glycerol concentration is 55wt% to 65wt%, and preferably when the concentration is 60wt%, the capsaicin content in the obtained extract is higher.
[0036] Compared with Example 5, soybean oil was added during the alcohol extraction of pepper in Examples 8 to 11, and the capsaicin content of Examples 8 to 11 was better than that of Example 5, indicating that the addition of soybean oil can improve the effective dissolution of capsaicin in pepper. In Examples 8 to 11, different masses of ethanol and soybean oil were added, and the capsaicin content of Examples 9 and 10 was better than that of Examples 8 and 11, indicating that when the mass ratio of ethanol to soybean oil was 5:2 to 3, the capsaicin content in the obtained pepper extract was higher.
[0037] Compared with Example 2, Comparative Example 2 first performs glycerol extraction and then alcohol extraction. As a result, the capsaicin content of Example 2 is better than that of Comparative Example 2, indicating that when the extraction order is alcohol extraction first and then glycerol extraction, the capsaicin content in the obtained extract is higher.
[0038] Test 2: 200 14-day-old broilers of similar weight were selected as experimental subjects and randomly divided into 14 groups, 20 in each group, i.e., 1 control group and 13 test groups. The test groups were fed with the veterinary Chinese medicine oral liquid prepared in Examples 1 to 11 and Comparative Examples 1 to 2, twice a day, 0.5 mL of the Chinese medicine oral liquid was diluted with 1.8 L of water each time in each test group, and the feeding was continued for 21 days. The weight changes and feed consumption of each group of test broilers were recorded, and the feed-to-weight ratio was calculated, feed-to-weight ratio=average feed consumption / average weight gain.
[0039] The test results are shown in Table 2 below: Table 2 Clinical trial results of veterinary Chinese medicine oral liquids prepared in the control group, Examples 1 to 11 and Comparative Examples 1 to 2
[0040] Compared with the control group, the experimental group was fed with veterinary Chinese medicine oral liquid every day. The results showed that the average weight gain of the experimental group was greater than that of the control group, and the average feed consumption and feed-to-weight ratio were both lower than those of the control group, indicating that veterinary Chinese medicine oral liquid has the effect of promoting the growth of broilers.
[0041] Compared with Comparative Example 1, Example 2 adds the step of glycerol extraction of peppers, and the average weight gain of Example 2 is greater than that of Comparative Example 1, and the average feed consumption and feed weight ratio are both less than those of Comparative Example 1, indicating that the step of glycerol extraction of peppers can increase the types and contents of active substances in the oral liquid, and enhance the efficacy of veterinary Chinese medicine oral liquid in promoting the rapid growth of broilers. In Examples 2 and Examples 4 to 7, different concentrations of glycerol are added to extract peppers, and the average weight gain of Examples 4 to 6 is greater than that of Examples 2 and 7, and the average feed consumption and feed weight ratio are both less than those of Examples 2 and 7, indicating that when the concentration of glycerol added is 55wt% to 65wt%, and the preferred concentration is 60wt%, the obtained veterinary Chinese medicine oral liquid has a better efficacy in promoting the rapid growth of broilers.
[0042] Compared with Example 5, soybean oil was added during the alcohol extraction of pepper in Examples 8 to 11. As a result, the average weight gain of Examples 8 to 11 was greater than that of Example 5, and the average feed consumption and feed-to-weight ratio were both less than those of Example 5, indicating that the addition of soybean oil can improve the effective dissolution of capsaicinoid components in pepper and increase the types and contents of active substances in the oral liquid. In Examples 8 to 11, ethanol and soybean oil of different masses were added. As a result, the average weight gain of Examples 9 and 10 was greater than that of Examples 8 and 11, and the average feed consumption and feed-to-weight ratio were both less than those of Examples 8 and 11, indicating that when the mass ratio of ethanol to soybean oil was 5:2 to 3, the obtained veterinary Chinese medicine oral liquid had a better effect in promoting the rapid growth of broilers.
[0043] Compared with Comparative Example 2, Example 2 first performs alcohol extraction on the pepper and then performs glycerol extraction. As a result, the average weight gain of Example 2 is greater than that of Comparative Example 2, and the average feed consumption and feed-to-weight ratio are both less than those of Example 2, indicating that when the extraction order is alcohol extraction first and then glycerol extraction, the obtained veterinary Chinese medicine oral liquid has a better effect in promoting the rapid growth of broilers.
[0044] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.
Claims
1. A veterinary Chinese medicine oral liquid for promoting the growth of broiler chickens. It is characterized in that The raw materials include the following components in parts by weight: 10-30 parts of pepper, 60-80 parts of mushroom stems, 20-40 parts of hawthorn, 30-50 parts of fried malt, 20-40 parts of fried yam, 20-40 parts of atractylodes macrocephala, and 10-30 parts of roasted licorice.
2. The veterinary Chinese medicine oral liquid for promoting the growth of broiler chickens according to claim 1, It is characterized in that The raw materials include the following components in parts by weight: 15-25 parts of pepper, 70-80 parts of mushroom stems, 30-40 parts of hawthorn, 35-45 parts of fried malt, 25-23 parts of fried yam, 25-30 parts of atractylodes macrocephala, and 10-20 parts of roasted licorice.
3. A method for preparing a veterinary Chinese medicine oral liquid for promoting broiler growth according to claim 1 or 2, It is characterized in that The following steps are involved: S1, adding pepper to an extractant for extraction, filtering to obtain a drug residue and a filtrate, and concentrating the filtrate under reduced pressure to obtain an extract; S2, soaking the remaining raw materials in water, adding the medicinal residue, decoction by water extraction to obtain a decoction, and concentrating under reduced pressure and filtering to obtain a clear liquid; S3, after mixing the extract with the clear liquid, adding auxiliary materials to prepare the liquid, packaging and sterilizing to obtain a finished veterinary Chinese medicine oral liquid; The auxiliary materials include 0.01 g / mL calcium propionate, 0.0003 g / mL Tween 80, and 0.0001 g / mL PEG400.
4. A method for preparing a veterinary Chinese medicine oral liquid for promoting broiler growth according to claim 3, It is characterized in that The extraction of the pepper is carried out in two steps, firstly alcohol extraction and then glycerol extraction.
5. A method for preparing a veterinary Chinese medicine oral liquid for promoting broiler growth according to claim 4, It is characterized in that The glycerol concentration used in the glycerol extraction is 55wt%~65wt%.
6. A method for preparing a veterinary Chinese medicine oral liquid for promoting broiler growth according to claim 4, It is characterized in that The ethanol concentration used in the alcohol extraction is 80wt%, the temperature of the alcohol extraction is 33-37°C, and the time is 5.5-6.5h.
7. A method for preparing a veterinary Chinese medicine oral liquid for promoting broiler growth according to claim 4, It is characterized in that During the alcohol extraction, the extractant also includes soybean oil, and the mass ratio of the ethanol to the soybean oil is 5:1-3.
8. The method for preparing the veterinary Chinese medicine oral liquid for promoting the growth of broiler chickens according to claim 3, It is characterized in that The water decoction method is carried out twice, the first water decoction time is 1.5-2.5 hours, and the second water decoction time is 45-75 minutes.
9. The method for preparing a veterinary Chinese medicine oral liquid for promoting broiler growth according to claim 3, It is characterized in that The concentrated drug content of the decoction is 1 g / mL.
10. Use of the veterinary Chinese medicine oral liquid for promoting broiler growth according to any one of claims 1 to 2 or the veterinary Chinese medicine oral liquid for promoting broiler growth obtained by the preparation method according to any one of claims 3 to 9 in the field of broiler breeding.