Private part nursing product using active probiotics

Abstract

The invention relates to a private part care product using active probiotics, the private part care product contains polymer absorption resin or wood pulp, the private part care product is characterized in that microcapsules are added into the polymer absorption resin or wood pulp, and the content of the microcapsules comprises the active probiotics; the microcapsule wall material is prepared from oligosaccharide, sodium alginate and chitosan. The active probiotics are used, the microcapsules are used for embedding the active probiotics, the microcapsules are uniform in particle size distribution and easy to prepare, it can be ensured that the probiotics are rapidly dissolved in an acid environment with the pH value being 3.8-4.5 and act on private parts in time, and the active action rate of the active probiotics during external application is effectively increased.

Description

Technical Field

[0001] The invention relates to a private parts care product, in particular to a private parts care product using active probiotics. Background Art

[0002] The female private parts are mainly composed of lactobacilli, which constitute the vaginal microecological barrier and play a key role in regulating the balance of vaginal microecology. Almost all reproductive tract infections are accompanied by changes in the female vaginal microenvironment. Some probiotics have an auxiliary effect on women's recovery from reproductive infections.

[0003] At present, most of the probiotics added to sanitary napkins are inactivated, and it is difficult to determine whether these metabolites are effective for private health, and may even cause adverse reactions such as allergies; active probiotics are easily inactivated by conditions such as temperature, humidity, and pH, and sanitary napkins are in a dry environment, so how to add active probiotics becomes a difficult point; in addition, after active probiotics are activated, their life cycle is extremely short due to lack of carbon source, and their survival possibility is very small, and their efficacy also needs to be considered. Summary of the invention

[0004] In order to solve the problem of low activity of active probiotics added to sanitary napkins in the prior art, the present invention provides a private area care product using active probiotics.

[0005] The present invention discloses a private area care product using active probiotics, which contains a polymer absorbent resin or wood pulp. The product is characterized in that microcapsules are added into the polymer absorbent resin or wood pulp, and the contents of the microcapsules include active probiotics; the wall material of the microcapsules is composed of oligosaccharides, sodium alginate and chitosan.

[0006] Specifically, the active probiotics include but are not limited to one or more of Bifidobacterium lactis, Lactobacillus acidophilus, Lactobacillus bulgaricus, Lactobacillus pseudocasei, Lactobacillus fermentum, Lactobacillus rhamnosus, Bifidobacterium bifidum, Bifidobacterium longum, Bifidobacterium lactis, Lactobacillus plantarum or Lactobacillus paracasei.

[0007] The content of the microcapsule also includes glycerin, matrine, phellodendron amurense extract and cnidium monnieri extract.

[0008] Furthermore, in parts by weight, the added amounts of the contents are 2-4% active probiotics, 2-5% glycerol, 0.01-0.3% matrine, 0.08-0.1% phellodendron extract and 3-5% cnidium monnieri extract.

[0009] The microcapsule wall material adopts a wall material composition, which is composed of oligosaccharides, sodium alginate and chitosan in a mass ratio of 2-3:2-3:2; in terms of weight, the added amount of the wall material composition is 85.6-92.91%.

[0010] A method for preparing a private parts care product using active probiotics, characterized by comprising the following steps: S1: Weigh an appropriate amount of active probiotic liquid, put it into a centrifuge for high-speed centrifugation, and take out the precipitated active probiotic powder after the centrifugation is completed; S2: Mixing the active probiotic powder and glycerin and stirring at high speed to form an active probiotic glycerin mixture; S3: Weigh the wall material in proportion in a cup, mix with the active probiotic glycerin mixture and matrine, Phellodendron amurense extract, and Cnidium monnieri extract, and stir at high speed; S4: the mixed solution obtained in S3 is subjected to low-temperature vacuum spray drying to prepare active probiotic microcapsules; S5: Add active probiotic microcapsules to high molecular absorbent resin or wood pulp of private care products.

[0011] Specifically, the low-temperature vacuum spray drying method in S4 is: atomize the mixed liquid in the drying chamber, introduce dry gas at the same time and use a vacuum pump to generate negative pressure in the drying chamber to promote the evaporation of water contained in the material, so as to reduce the required temperature of the drying gas. The particle size of the atomized material is 40-20μm, the vacuum degree in the drying chamber is -0.1~-0.01Mpa, the inlet temperature of the drying chamber is 30~40℃, and the outlet temperature of the drying chamber is 30~35℃. The particle size of the active probiotic microcapsules dispersed in water is 300~100nm.

[0012] Specifically, the adding method in S5 is: fully mix the active probiotic microcapsules with the polymer absorbent resin to form a polymer mixture, then break up and mix the polymer mixture with wood pulp, roll and cut it into shape to obtain an absorbent core, and finally compound it with the surface layer, guide layer and bottom film to obtain private care products.

[0013] The method of adding S5 can also be: spraying active probiotic microcapsules onto a mixed core of polymer absorbent resin and wood pulp, then wrapping it with dust-free paper and cutting it into an absorbent core, and finally compounding it with a surface layer, a guide layer and a bottom film to produce a private care product.

[0014] The method of adding S5 can also be: mixing active probiotic microcapsules with dust-free paper, fiber and polymer absorbent resin to prepare active probiotic absorbent paper, and finally compounding it with the surface layer, guide layer and bottom film to prepare private care products.

[0015] The present invention has the following advantages: 1) The present invention uses active probiotics and uses microcapsules to encapsulate the active probiotics. At present, the methods of microcapsule encapsulation include freeze-drying, spray drying, emulsification and other methods. However, the freeze-drying method has a relatively long drying time and high energy consumption, resulting in high costs; the microcapsules formed by the emulsification method have a liquid core, are small in size, and have uneven shapes and sizes. The microcapsules prepared by the spray drying method generally have a uniform morphology and particle size distribution, and are simple to prepare, so the spray drying method is selected. In addition, the spray drying method can ensure that the probiotics are quickly dissolved in an acidic environment with a pH value of 3.8 to 4.5 and act on the private parts in a timely manner, effectively improving the active action rate of active probiotics when used externally.

[0016] 2) The processing of active probiotic microcapsules is completed in a low-temperature environment, which effectively reduces the loss of active probiotics during the microcapsule production process and improves the storage stability of the microcapsules.

[0017] 3) The wall materials use oligosaccharides, sodium alginate and chitosan, which can reduce production costs; at the same time, compared with other wall material components, the wall material combination used in the present invention is stable in storage and dissolves slowly in an acidic environment with a pH value of 3.8 to 4.5. DETAILED DESCRIPTION

[0018] Embodiment 1: A method for preparing active probiotic microcapsules for private care products, characterized by comprising the following steps: The preparation method of a private parts care product using active probiotics is characterized by comprising the following steps: S1: Weigh an appropriate amount of active probiotic liquid, put it into a centrifuge for high-speed centrifugation, and take out the precipitated active probiotic powder after the centrifugation is completed; S2: mixing active probiotics and glycerol in a mass ratio of 1:1, and stirring at a high speed to form an active probiotic glycerol mixture; S3: Weigh 92.91% of the wall material in a cup according to the proportion, mix with 4% of active probiotic glycerin mixture, 0.01% of matrine, 0.08% of Cortex Phellodendri extract, and 3% of Cnidium monnierie extract, and stir at high speed; S4: atomizing the mixed liquid obtained in S3 in a drying chamber, while introducing drying gas and using a vacuum pump to generate negative pressure in the drying chamber to promote evaporation of water contained in the material, so as to reduce the required temperature of the drying gas. The particle size of the atomized material is 40-20 μm, the vacuum degree in the drying chamber is -0.1 to -0.01 MPa, the air inlet temperature of the drying chamber is 30 to 40° C., and the air outlet temperature of the drying chamber is 30 to 35° C. The mixed liquid is dried to obtain active probiotic microcapsules; S5: The active probiotic microcapsules are fully mixed with the polymer absorbent resin to form a polymer mixture, and then the polymer mixture is dispersed and mixed with wood pulp, and the mixture is cut by rollers to form an absorbent core, and finally the absorbent core is compounded with the surface layer, the guide layer and the bottom film to obtain the private care product.

[0019] Effect verification: 1) Particle size: Select 5 batches of samples, dissolve the microcapsules in pure water at a weight ratio of 1:25, and prepare a total of five samples. After the particles are completely dissolved, use a light-dependent nanoparticle size distribution instrument to measure the particle sizes of samples 1 to 5 at 25°C. The results are as follows: 2) Determination of active probiotic content: 2.1 Sample pretreatment: Place the microcapsules in a sterilized bottle containing glass beads, add 0.85% sterile saline to dilute 100 times, grind with a grinding bowl for 10 minutes and let stand for 1 hour, take 500uL of the solution into a 1.5ml EP tube for subsequent experiments; 2.2 Preparation of DNA template for test sample: Take the above treated solution and perform DNA extraction according to the instructions of the nucleic acid extraction kit. The extracted DNA sample is tested for the concentration and purity of nucleic acid using a nucleic acid quantifier; 2.3 Preparation of negative control DNA template: Place the negative control group of microcapsules without active probiotics into a sterilized bottle containing glass beads, add 0.85% sterile saline to dilute it 100 times, grind it with a grinding bowl for 10 minutes, let it stand for 1 hour, and take 500uL into a 1.5mL EP tube for subsequent experiments.

[0020] 2.4 Preparation of blank control DNA template: Take 500uL of 0.85% sterile saline into a 1.5mL EP tube for subsequent experiments.

[0021] 2.5 Preparation of droplet digital PCR reaction system: Three parallel droplet digital PCR reactions were set up for each sample, and the PCR reaction system was set up as follows: 2.7 Result calculation formula: Sample bacterial concentration (cells / g) = (20 μL × copy number × volume of extracted DNA solution × dilution factor) / (volume of DNA added to the 20 μL system × mass of sample used for DNA extraction).

[0022] 2.8 Results Analysis and Presentation 2.8.1 Threshold setting: Set the fluorescence threshold value according to the endpoint fluorescence value of the negative amplification system in the system. The threshold value needs to clearly distinguish between negative droplets and positive droplets in the amplification system.

[0023] 2.8.2 Analysis of the test results of the control group: There was no amplification phenomenon in the negative control group and the blank control group, indicating that the droplet digital PCR method was normal and did not need to be repeated, which was credible.

[0024] 2.8.3 Analysis of sample test results The results of the sample test are analyzed according to the following principles: the positive control and the test sample both show obvious amplification, and the fluorescence signal value of the amplification endpoint exceeds the fluorescence threshold value, which indicates that the target bacteria are detected in the sample, which is expressed as "the target bacteria are detected in the sample, and the test result is positive". The concentration of bacteria in the sample is calculated according to the calculation formula, and the unit is / g.

[0025] 2.8.4 Test results (pieces / g): 3) Antibacterial experiment of probiotics with different contents 3.1 Test bacteria species: Bacteria: Escherichia coli (8099 or CICC 10899) or Escherichia coli (ATCC 25922), Staphylococcus aureus (ATCC 6538 or ATCC 25923).

[0026] Yeast: Candida albicans (ATCC 10231). 3.2 Preparation of test bacterial suspension: Take the bacteria stored under frozen conditions, open it under aseptic operation, add an appropriate amount of nutrient broth, and gently blow and aspirate several times to melt and disperse the bacteria. Take a test tube containing 5.0 mL~10.0 mL nutrient broth medium, drop a small amount of bacterial suspension, and culture at 36℃±1℃ for 18h~24h. Use an inoculation loop to take the bacterial suspension of the first generation culture after cultivation, streak it on the nutrient agar medium plate, and culture it at 36℃±1℃ for 18h~24h. Pick the typical colonies in the above second generation culture, inoculate them on the nutrient agar slant, and culture them at 36℃±1℃ for 18h~24h to obtain the third generation culture. Take fresh slant cultures (18 h to 24 h) of the 3rd to 6th generation of the strain, wash the bacterial lawn with 5.0 mL 0.03 mol / L PBS solution, make the bacterial suspension evenly, and then dilute it with PBS to the required concentration.

[0027] 3.3 Culture medium: Nutrient agar medium for bacterial culture, Sabre weak agar medium for yeast culture; nutrient broth medium, Sabre weak liquid medium.

[0028] 3.4 Dilution solution: 0.03 mol / L PBS, pH 7.2; 3.5 Neutralizing agent: prepared with PBS; 3.6 Test steps: Take the 24 h fresh slant culture of the test bacteria, wash it with PBS, dilute it with PBS to 5.0×105 CFU / mL~5.0×106 CFU / mL, and prepare a bacterial suspension for use. Use sterile scissors to select 20 mm×30 mm samples with microcapsule addition amounts of 0 / g (positive control sample), 0.2 / g (sample 1), 0.3 / g (sample 2) and 0.4 / g (sample 3) under sterile conditions for use. Add 100 μL of bacterial suspension during the test. The control sample must be sterilized by pressure steam before infection. Take a sterile plate, use sterile tweezers to take 2 test samples, do not overlap, place in a 20 ℃±1 ℃ water bath for 5 min, add 100 μL of test bacterial suspension on each sample, and start the time immediately. After the test bacteria and the sample have been in contact for the time specified in the instructions, pick up the infected sample and add it to a 5.0mL PBS test tube and mix it. After oscillation and elution, take 1.0mL of sample solution respectively, and determine the number of surviving bacteria according to the live bacteria culture counting method. Inoculate 2 plates with each tube of sample solution. If the number of colonies growing on the plate is large, it can be diluted 10 times in series and then counted for live bacteria culture. At the same time, use 2 control samples with the same material as the test sample and without microcapsules to replace the test sample for the test as a positive control. The amount of bacteria recovered in the positive control is 1.0×10 4 CFU / piece~9.0×10 4 CFU / tablet.

[0029] 3.7 Calculation of inhibition rate: All test samples and control samples were cultured at 36℃±1℃ for 48 h (bacteria) or 72 h (yeast) and the viable colonies were counted. The test was repeated 3 times and the inhibition rate was calculated. Where: Y ─Inhibition rate; N c ─ Average colony count of control samples, in colony forming units per piece (CFU / piece); N s─average colony count of the test sample, in colony forming units per piece (CFU / piece).

[0030] 3.8 Result determination Antibacterial rate of each test YIf the rate is between 50% or more and less than 90%, it is considered to have antibacterial effect; if the antibacterial rate of each test is greater than or equal to 90%, it is considered to have strong antibacterial effect. The test results are as follows: Conclusion: The private care products without the addition of the present invention have no effect of inhibiting Candida albicans, Staphylococcus aureus and Escherichia coli, while the private care products with an addition amount of 0.2g / piece have an antibacterial effect, and the private care products with an addition amount of 0.4g / piece have a strong inhibitory effect on Candida albicans, Staphylococcus aureus and Escherichia coli.

Claims

1. A private parts care product using active probiotics, the private parts care product containing a polymer absorbent resin or wood pulp, Features Microcapsules are added into high molecular absorbent resin or wood pulp, wherein the contents of the microcapsules include active probiotics; and the wall material of the microcapsules is composed of oligosaccharides, sodium alginate and chitosan.

2. A private parts care product using active probiotics as claimed in claim 1, Features Active probiotics include, but are not limited to, one or more of Bifidobacterium lactis, Lactobacillus acidophilus, Lactobacillus bulgaricus, Lactobacillus pseudocasei, Lactobacillus fermentum, Lactobacillus rhamnosus, Bifidobacterium bifidum, Bifidobacterium longum, Bifidobacterium lactis, Lactobacillus plantarum or Lactobacillus paracasei.

3. A private parts care product using active probiotics as claimed in claim 1, Features The contents of the microcapsule also include glycerin, matrine, phellodendron amurense extract and cnidium monnieri extract.

4. A private parts care product using active probiotics as claimed in claim 3, Features In terms of weight, the added amounts of the contents are 2-4% active probiotics, 2-5% glycerol, 0.01-0.3% matrine, 0.08-0.1% phellodendron extract and 3-5% cnidium monnieri extract.

5. A private parts care product using active probiotics as claimed in claim 1, Features The microcapsule wall material adopts a wall material composition, which is composed of oligosaccharide, sodium alginate and chitosan in a mass ratio of 2-3:2-3:2; in terms of weight, the addition amount of the wall material composition is 85.6-92.91%.

6. A method for preparing a private care product using active probiotics, Features The following steps are involved: S1: Weigh an appropriate amount of active probiotic liquid, put it into a centrifuge for high-speed centrifugation, and take out the precipitated active probiotic powder after the centrifugation is completed; S2: Mixing the active probiotic powder and glycerin and stirring at high speed to form an active probiotic glycerin mixture; S3: Weigh the wall material in proportion in a cup, mix with the active probiotic glycerin mixture and matrine, Phellodendron amurense extract, and Cnidium monnieri extract, and stir at high speed; S4: the mixed solution obtained in S3 is subjected to low-temperature vacuum spray drying to prepare active probiotic microcapsules; S5: Add active probiotic microcapsules to high molecular absorbent resin or wood pulp of private care products.

7. A method for preparing a private parts care product using active probiotics as claimed in claim 6, Features The method of low-temperature vacuum spray drying in S4 is: atomize the mixed liquid in the drying chamber, introduce dry gas at the same time and use a vacuum pump to generate negative pressure in the drying chamber to promote the evaporation of water contained in the material, so as to reduce the required temperature of the drying gas. The particle size of the atomized material is 40-20μm, the vacuum degree in the drying chamber is -0.1~-0.01Mpa, the inlet temperature of the drying chamber is 30~40℃, and the outlet temperature of the drying chamber is 30~35℃.

8. A method for preparing a private parts care product using active probiotics as claimed in claim 6, Features The adding method in S5 is: fully mix the active probiotic microcapsules with the polymer absorbent resin to form a polymer mixture, then break up and mix the polymer mixture with wood pulp, roll and cut into shape to obtain an absorbent core, and finally compound with the surface layer, guide layer and bottom film to obtain private care products.

9. A method for preparing a private parts care product using active probiotics as claimed in claim 6, Features The adding method in S5 is to spray active probiotic microcapsules onto a mixed core of polymer absorbent resin and wood pulp, then wrap it with dust-free paper and cut it into an absorbent core, and finally compound it with a surface layer, a guide layer and a bottom film to prepare a private care product.

10. A method for preparing a private parts care product using active probiotics as claimed in claim 6, Features The adding method in S5 is to mix active probiotic microcapsules with dust-free paper, fiber and polymer absorbent resin to prepare active probiotic absorbent paper, and finally compound it with the surface layer, guide layer and bottom film to prepare private care products.

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