Chiral compound separation and collection device

Abstract

The invention relates to the technical field of chiral compound separation and collection, and discloses a chiral compound separation and collection device, which comprises a pretreatment mechanism, a purification and separation mechanism and a collection mechanism, the purification and separation mechanism comprises a chromatographic column assembly and a chiral column assembly, the chromatographic column assembly and the chiral column assembly are connected in series, the chromatographic column assembly is used for separating a target compound from a pretreatment sample solution, and the chiral column assembly is used for separating a chiral compound from the target compound; and the collecting mechanism is used for collecting the chiral compound separated by the chiral column assembly. The method is used for solving the problem that a relatively pure chiral compound cannot be obtained by directly separating the compound by adopting a chiral column in the prior art.

Description

Technical Field

[0001] The present invention relates to the technical field of separation and collection of chiral compounds, and in particular to a device for separation and collection of chiral compounds. Background Art

[0002] Chiral compounds are compounds with the same molecular weight and structure but opposite left-right arrangements, such as the real object and its mirror image.

[0003] Some natural plants contain a small amount of chiral compounds, which are of very high value. For example, natural plants contain a small amount of taxane, which is an intermediate for the synthesis of the high-value drug paclitaxel.

[0004] The separation and collection of chiral compounds mainly includes pretreatment, purification and collection. Pretreatment is to remove impurities and concentrate the sample liquid after extraction to obtain a pretreated sample liquid. Purification is to purify the pretreated sample liquid to separate chiral compounds. Collection is to collect the separated chiral compounds.

[0005] In the prior art, during purification, a chiral preparative chromatographic column is usually used to directly separate the chiral compounds in the pretreated sample solution. The separated samples still need to be concentrated, the solvent removed, etc., and relatively pure chiral compounds cannot be directly obtained. In addition, the chiral preparative chromatographic column has a large loss. Summary of the invention

[0006] In view of this, an object of the present invention is to provide a chiral compound separation and collection device to solve the problem in the prior art that relatively pure chiral compounds cannot be obtained by directly using chiral columns to separate compounds.

[0007] The present invention solves the above technical problems by the following technical means:

[0008] A chiral compound separation and collection device comprises a pretreatment mechanism, a purification and separation mechanism and a collection mechanism, wherein the pretreatment mechanism is used for removing impurities and concentrating a sample liquid to obtain a pretreatment sample liquid, the purification and separation mechanism comprises a chromatographic column assembly and a chiral column assembly, the chromatographic column assembly and the chiral column assembly are connected in series, the chromatographic column assembly is used for separating a target compound from the pretreatment sample liquid, the chiral column assembly is used for separating a chiral compound from the target compound, and the collection mechanism is used for collecting the chiral compound separated by the chiral column assembly.

[0009] Furthermore, a first electronic gas circuit controller is arranged between the chromatographic column assembly and the chiral column assembly, the chromatographic column assembly includes at least two chromatographic columns, the chiral column assembly includes a chiral column, both of the chromatographic columns are connected to the chiral column through the first electronic gas circuit controller, and the first electronic gas circuit controller is used to control the connection and disconnection of the chromatographic column and the chiral column.

[0010] Furthermore, the first electronic gas circuit controller is connected to a first detector, and the first detector is used to detect and identify the components after separation of the pre-treated sample liquid.

[0011] Furthermore, a second electronic gas circuit controller is provided between the chiral column and the collecting mechanism, and the second electronic gas circuit controller is used to control the on-off of the chiral column and the collecting mechanism.

[0012] Furthermore, the second electronic gas circuit controller is connected to a second detector, and the second detector is used to detect and identify the components after separation of the target compound.

[0013] Furthermore, the collection mechanism includes at least two electrostatic capture cold traps.

[0014] Furthermore, the pretreatment mechanism includes a filtering component and a fractionation component, wherein the filtering component is used to filter the sample liquid to obtain a pure sample liquid, and the fractionation component is used to preliminarily remove non-target compounds in the pure sample liquid to obtain a pretreated sample liquid.

[0015] Furthermore, the filter assembly includes a filter cartridge, a piston, a collecting cartridge, a driving member and a blocking cartridge. The filter cartridge is fixedly disposed in the collecting cartridge, the collecting cartridge is used to collect pure sample liquid filtered out by the filter cartridge, the driving member is fixedly installed on the top of the collecting cartridge, the driving member is transmission-connected to the piston, the driving member is used to drive the piston to move up and down in the filter cartridge, a plurality of evenly distributed filter holes are provided on the cartridge wall of the filter cartridge, when the piston moves downward, the piston filters the sample liquid out from the filter holes of the filter cartridge, the blocking cartridge is slidably disposed in the collecting cartridge, a ventilation area is provided between the piston and the blocking cartridge, when the piston and the blocking cartridge move upward synchronously, the blocking cartridge blocks the filter holes in the blocking cartridge.

[0016] Furthermore, a transmission member is provided at the bottom of the piston, and the transmission member passes through the filter cylinder. A one-way limit member is provided at the bottom of the blocking cylinder. When the transmission member is engaged with the one-way limit member, the blocking cylinder moves up synchronously with the filter cylinder.

[0017] Furthermore, the one-way limit member includes a first elastic ring, which is fixedly connected to the inner wall of the sealing tube; the transmission member includes a transmission frame, which is fixedly connected to the piston; a second elastic ring is arranged on the transmission frame; and limit rings are arranged at both the upper and lower ends of the collecting tube; when the sealing tube abuts against the limit ring, the piston drives the second elastic ring to squeeze and pass through the first elastic ring through the transmission frame, thereby realizing the engagement or disengagement of the second elastic ring with the first elastic ring.

[0018] Beneficial effects of the present invention:

[0019] 1. By setting the chromatographic column assembly and the chiral column assembly in series, the target compound is first separated from the pretreated sample liquid by the chromatographic column assembly, and then the chiral compound is separated from the target compound by the chiral column assembly, which can not only improve the separation purity of the chiral compound, but also reduce the loss of the chiral column.

[0020] 2. By setting up multiple chromatographic columns and the first electronic gas path controller, different chromatographic columns are switched to provide the target compound to the chiral column, which effectively reduces the waiting time of the chiral column and improves the separation efficiency.

[0021] 3. By setting up a second electronic gas circuit controller, a second detector and an electrostatic capture cold trap, different chiral compounds can be respectively introduced into different electrostatic capture cold traps for collection.

[0022] 4. By setting up a filter cylinder, a piston, a collecting cylinder, a driving part and a sealing cylinder, when the piston and the sealing cylinder move up synchronously, the sealing cylinder will block the filter holes in the sealing cylinder, and the external gas will enter the filter cylinder from the filter holes in the ventilation area, thereby unblocking the blocked filter holes to ensure the filtering effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 It is a principle block diagram of an embodiment of a chiral compound separation and collection device of the present invention;

[0024] Figure 2 It is a schematic diagram of the connection structure of the purification and separation mechanism and the collection mechanism of an embodiment of a chiral compound separation and collection device of the present invention;

[0025] Figure 3 It is a cross-sectional structural schematic diagram of a filtering component in a filtering state of an embodiment of a chiral compound separation and collection device of the present invention;

[0026] Figure 4 It is a schematic cross-sectional structure diagram of a filtering component in a state of filtering holes in an embodiment of a chiral compound separation and collection device of the present invention;

[0027] Figure 5It is a schematic diagram of the cross-sectional structure of a filter assembly of an embodiment of a chiral compound separation and collection device of the present invention;

[0028] in,

[0029] 1. Pretreatment mechanism; 11. Filtration component; 12. Fractionation component;

[0030] 2. Purification and separation mechanism; 211. first chromatographic column; 212. second chromatographic column; 22. chiral column assembly;

[0031] 3. Collection mechanism; 31. Electrostatic capture cold trap;

[0032] 41. first electronic gas circuit controller; 411. first detector; 42. second electronic gas circuit controller; 421. second detector;

[0033] 51. filter cartridge; 511. filter hole; 52. piston; 53. collection cartridge; 531. stop ring; 54. drive member; 55. plugging cartridge; 56. ventilation area; 57. return spring;

[0034] 61, transmission member; 611, transmission frame; 612, second elastic ring; 62, one-way limiting member; 621, first elastic ring;

[0035] 7. Cover body; 71. Liquid inlet pipe; 72. Vent hole. DETAILED DESCRIPTION

[0036] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0037] like Figure 1-Figure 5 As shown, a chiral compound separation and collection device of the present invention comprises a pretreatment mechanism 1, a purification and separation mechanism 2 and a collection mechanism 3, wherein the pretreatment mechanism 1 is used to remove impurities and concentrate the sample liquid to obtain a pretreatment sample liquid, the purification and separation mechanism 2 comprises a chromatographic column assembly and a chiral column assembly 22, the chromatographic column assembly is connected in series with the chiral column assembly 22, the chromatographic column assembly is used to separate the target compound from the pretreatment sample liquid, the chiral column assembly 22 is used to separate the chiral compound from the target compound, and the collection mechanism 3 is used to collect the chiral compound separated by the chiral column assembly 22. By arranging the chromatographic column assembly and the chiral column assembly 22 in series, the target compound is first separated from the pretreatment sample liquid by the chromatographic column assembly, and then the chiral compound is separated from the target compound by the chiral column assembly 22, which can not only improve the separation purity of the chiral compound, but also reduce the loss of the chiral column.

[0038] In this embodiment, a first electronic gas circuit controller 41 is arranged between the chromatographic column assembly and the chiral column assembly 22. The chromatographic column assembly includes at least two chromatographic columns. This embodiment is described by taking the first chromatographic column 211 and the second chromatographic column 212 as examples. The chiral column assembly 22 includes a chiral column. The first chromatographic column 211 and the second chromatographic column 212 are both connected to the chiral column through the first electronic gas circuit controller 41. The first electronic gas circuit controller 41 is used to control the connection and disconnection between the chromatographic column and the chiral column.

[0039] In this embodiment, the retention time of the target compound during chromatographic column separation is 20 minutes, and during chiral column separation, the chiral compounds to be collected include a first chiral compound and a second chiral compound, and the retention time of the first chiral compound is 9 minutes and the retention time of the second chiral compound is 10 minutes.

[0040] When in use, a preset amount of pre-treated sample liquid is first injected into the first chromatographic column 211 through the injection port, and the pre-treated sample liquid is separated by the first chromatographic column 211. During the separation process, the first electronic gas circuit controller 41 controls the first chromatographic column 211 to be connected to the waste liquid tank, and the separated unnecessary waste liquid is discharged into the waste liquid tank. And when it reaches 19.5min, the first electronic gas circuit controller 41 connects the first chromatographic column 211 with the chiral column, so that the target compound enters the chiral column, and the target compound is separated by the chiral column to obtain a chiral compound. And at 20.5min, that is, after the target compound enters the chiral column, the first chromatographic column 211 is connected to the waste liquid cylinder.

[0041] Since the separation speed of the chiral column is faster than that of the chromatographic column, during the separation process of the first chromatographic column 211, after 10 minutes, a preset amount of pre-treated sample liquid is injected into the second chromatographic column 212 through the injection port, and the pre-treated sample liquid is separated by the second chromatographic column 212 to ensure that the second chromatographic column 212 can connect with the first chromatographic column 211 to provide the target compound for the chiral column, effectively reducing the waiting time of the chiral column and improving the separation efficiency. In fact, the separation speed of the chiral column can also be adjusted to match the actual number of chromatographic columns and the retention time of the target compound to ensure the separation efficiency.

[0042] In this embodiment, the first electronic gas circuit controller 41 is connected to a first detector 411, which is used to detect and identify the components after the pre-treatment sample liquid is separated. Before the separation is implemented, a preset amount of pre-treatment sample liquid can be injected into the chromatographic column to detect the retention time of the target compound. When the subsequent separation is implemented, the sample connected to the waste liquid barrel can be intermittently connected to the detector to monitor whether the separation effect of the chromatographic column decreases and adjust the injection time or the separation speed of the chiral column at any time.

[0043] In this embodiment, a second electronic gas circuit controller 42 is provided between the chiral column and the collecting mechanism 3, and the second electronic gas circuit controller 42 is used to control the on-off of the chiral column and the collecting mechanism 3. The collecting mechanism 3 includes at least two electrostatic capture cold traps 31. In fact, the number of corresponding electrostatic capture cold traps 31 can be selected according to the number of separated chiral compounds. This embodiment is described by taking the first electrostatic capture cold trap 31 and the second electrostatic capture cold trap 31 as examples.

[0044] After the target compound enters the chiral column for separation, after 8.5 minutes, the second electronic gas circuit controller 42 connects the chiral column with the first electrostatic capture cold trap 31, collects the first chiral compound through the first electrostatic capture cold trap 31, and after the collection is completed, the chiral column is connected to the waste liquid cylinder. After 9.5 minutes, the second electronic gas circuit controller 42 connects the chiral column with the second electrostatic capture cold trap 31, collects the second chiral compound through the second electrostatic capture cold trap 31, and after the collection is completed, the chiral column is connected to the waste liquid cylinder. In this embodiment, since there are only chiral compounds and no solvent when the chiral column is separated, after the chiral compound is collected, it can be directly emptied.

[0045] In this embodiment, the electrostatic capture cold trap includes a cylinder, a corona needle and a collecting cup. The corona needle softly ionizes the chiral compound entering the cylinder, so that the chiral compound carries a negative charge. The inner wall of the cylinder carries a positive charge and adsorbs the chiral compound. When the negative charge of the chiral compound is neutralized, the chiral compound will slide into the collecting cup at the bottom, thereby collecting and preserving the chiral compound.

[0046] In this embodiment, the second electronic gas circuit controller 42 is connected to a second detector 421, which is used to detect and identify the components of the target compound after separation. Before separation, a preset amount of the target compound can be injected into the chiral column to detect the retention time of different chiral compounds.

[0047] In this embodiment, the liquid can be injected into the injection port manually or by an injector. After adjusting various parameters such as the injection time, switching time, and chiral column separation speed, the system can be controlled by a computer control element to achieve continuous and automatic separation.

[0048] In this embodiment, the pretreatment mechanism 1 includes a filtering component 11 and a fractionation component 12. The filtering component 11 is used to filter the sample liquid to obtain a pure sample liquid, and the fractionation component 12 is used to preliminarily remove non-target compounds in the pure sample liquid to obtain a pretreated sample liquid. In this embodiment, the prior art of the fractionation component 12 is sufficiently disclosed, so it will not be described in detail. In some other embodiments, it can also be implemented by distillation and other methods.

[0049] In this embodiment, the filter assembly 11 includes a filter cartridge 51, a piston 52, a collection cartridge 53, a driving member 54 and a blocking cartridge 55. The filter cartridge 51 is fixedly disposed in the collection cartridge 53. The collection cartridge 53 is used to collect the pure sample liquid filtered out by the filter cartridge 51. The driving member 54 is fixedly mounted on the top of the collection cartridge 53. The driving member 54 is transmission-connected to the piston 52. The driving member 54 is used to drive the piston 52 to move up and down in the filter cartridge 51. The filter cartridge 51 is provided with a plurality of evenly distributed filter holes 511 on the cartridge wall. When the piston 52 moves downward, the piston 52 filters the sample liquid out from the filter hole 511 of the filter cylinder 51, and the blocking cylinder 55 is slidably arranged in the collecting cylinder 53. A ventilation area 56 is arranged between the piston 52 and the blocking cylinder 55. When the piston 52 and the blocking cylinder 55 move upward synchronously, the blocking cylinder 55 blocks the filter hole 511 in the blocking cylinder 55, and the external gas enters the filter cylinder 51 from the filter hole 511 in the ventilation area 56, thereby clearing the blocked filter hole 511 to ensure the filtering effect. And from bottom to top, the filter hole 511 on the wall of the filter cylinder 51 is cleared step by step, which effectively improves the clearing effect. In some other embodiments, the blocking cylinder 55 may not be designed, and it is only necessary to keep the filter cylinder 51 cleaned in time.

[0050] In this embodiment, a transmission member 61 is provided at the bottom of the piston 52, and the transmission member 61 penetrates the filter cartridge 51. A one-way stopper 62 is provided at the bottom of the plugging cartridge 55. When the transmission member 61 is engaged with the one-way stopper 62, the plugging cartridge 55 and the filter cartridge 51 are synchronously moved upward. After the filter cartridge 51 is moved upward, the one-way stopper 62 and the transmission member 61 are released, and the plugging cartridge 55 falls back under the action of gravity, so as to prevent the plugging cartridge 55 from affecting the subsequent filtering operation. In some other embodiments, an independent drive may also be provided for the plugging cartridge 55 to control the plugging cartridge 55 to move upward synchronously with the piston 52 and fall back independently.

[0051] In this embodiment, a return spring 57 is provided between the blocking cylinder 55 and the collecting cylinder 53 , and the return spring 57 is used to make the blocking cylinder 55 have a tendency to move away from the filtering cylinder 51 , so that the blocking cylinder 55 can fall back quickly.

[0052] In this embodiment, the one-way limit member 62 includes a first elastic ring 621, which is fixedly connected to the inner wall of the blocking tube 55. The transmission member 61 includes a transmission frame 611, which is fixedly connected to the piston 52. A second elastic ring 612 is arranged on the transmission frame 611. Limit rings 531 are arranged at the upper and lower ends of the collecting tube 53. When the blocking tube 55 abuts against the limit ring 531, the piston 52 drives the second elastic ring 612 to squeeze and pass through the first elastic ring 621 through the transmission frame 611, so as to realize the engagement or disengagement of the second elastic ring 612 and the first elastic ring 621. It should be noted that when the blocking tube 55 is not abutted against the limit ring 531, the friction force between the first elastic ring 621 and the second elastic ring 612 should ensure that the piston 52 can smoothly drive the blocking tube 55 to move upward through the first elastic ring 621 and the second elastic ring 612, so as to realize the blocking of the filter hole 511 by the blocking tube 55. In some other embodiments, the transmission member 61 may be configured as an electromagnet, and the one-way limiting member 62 may be configured as an iron block, so as to achieve engagement or disengagement of the one-way limiting member 62 and the transmission member 61 .

[0053] In this embodiment, a cover body 7 is provided on the top of the collecting cylinder 53, a limiting ring 531 at the upper end of the collecting cylinder 53 is provided on the cover body 7, the cover body 7 is fixedly connected to the collecting cylinder 53, the filter cylinder 51 is fixedly mounted on the cover body 7, and the driving member 54 is fixedly mounted on the cover body 7. A liquid inlet pipe 71 for injecting sample liquid into the filter cylinder 51 is provided on the cover body 7, and the liquid inlet pipe 71 penetrates the cover body 7 and the piston 52 and is inserted into the bottom of the filter cylinder 51. A plurality of vent holes 72 for balancing air pressure are also provided on the top of the cover body 7.

[0054] The working principle of the present invention is as follows:

[0055] The sample liquid is filtered and fractionated by the filtering component 11 and the fractionating component 12 to obtain a pure sample.

[0056] A preset amount of pre-treated sample liquid is injected into the first chromatographic column 211 through the injection port, and the pre-treated sample liquid is separated by the first chromatographic column 211. During the separation process, the first electronic gas circuit controller 41 controls the first chromatographic column 211 to be connected to the waste liquid tank, and the separated unnecessary waste liquid is discharged into the waste liquid tank. When it reaches 19.5 minutes, the first electronic gas circuit controller 41 connects the first chromatographic column 211 with the chiral column, so that the target compound enters the chiral column, and the target compound is separated by the chiral column to obtain a chiral compound. After the target compound enters the chiral column, the first chromatographic column 211 is connected to the waste liquid cylinder.

[0057] During the separation process of the first chromatographic column 211, after 10 minutes, a preset amount of pre-treated sample liquid is injected into the second chromatographic column 212 through the injection port, and the pre-treated sample liquid is separated by the second chromatographic column 212 to ensure that the second chromatographic column 212 can connect with the first chromatographic column 211 to provide the target compound for the chiral column. The first chromatographic column 211 and the second chromatographic column 212 are switched to provide the target compound for the chiral column.

[0058] After 8.5 minutes, the target compound enters the chiral column for separation. The second electronic gas circuit controller 42 connects the chiral column with the first electrostatic trap 31, collects the first chiral compound through the first electrostatic trap 31, and connects the chiral column with the waste liquid cylinder after the collection is completed. After 9.5 minutes, the second electronic gas circuit controller 42 connects the chiral column with the second electrostatic trap 31, collects the second chiral compound through the second electrostatic trap 31, and connects the chiral column with the waste liquid cylinder after the collection is completed.

[0059] The above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the present invention. Although the present invention is described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the purpose and scope of the technical solutions of the present invention, which should be included in the scope of the claims of the present invention. The techniques, shapes, and structural parts not described in detail in the present invention are all known technologies.

Claims

1. A chiral compound separation and collection device, characterized in that: The invention comprises a pretreatment mechanism (1), a purification and separation mechanism (2) and a collection mechanism (3); the pretreatment mechanism (1) is used for removing impurities and concentrating a sample liquid to obtain a pretreatment sample liquid; the purification and separation mechanism (2) comprises a chromatographic column component and a chiral column component (22); the chromatographic column component and the chiral column component (22) are connected in series; the chromatographic column component is used for separating a target compound from the pretreatment sample liquid; the chiral column component (22) is used for separating a chiral compound from the target compound; and the collection mechanism (3) is used for collecting the chiral compound separated by the chiral column component (22).

2. The chiral compound separation and collection device according to claim 1, characterized in that: A first electronic gas circuit controller (41) is arranged between the chromatographic column assembly and the chiral column assembly (22); the chromatographic column assembly comprises at least two chromatographic columns; the chiral column assembly (22) comprises a chiral column; both chromatographic columns are connected to the chiral column via the first electronic gas circuit controller (41); the first electronic gas circuit controller (41) is used to control the connection and disconnection between the chromatographic column and the chiral column.

3. The chiral compound separation and collection device according to claim 2, characterized in that: The first electronic gas circuit controller (41) is connected to a first detector (411), and the first detector (411) is used to detect and identify the components after separation of the pre-treated sample liquid.

4. The chiral compound separation and collection device according to claim 2, characterized in that: A second electronic gas circuit controller (42) is provided between the chiral column and the collecting mechanism (3), and the second electronic gas circuit controller (42) is used to control the on-off of the chiral column and the collecting mechanism (3).

5. The chiral compound separation and collection device according to claim 4, characterized in that: The second electronic gas circuit controller (42) is connected to a second detector (421), and the second detector (421) is used to detect and identify the components of the target compound after separation.

6. The chiral compound separation and collection device according to claim 1, characterized in that: The collecting mechanism (3) comprises at least two electrostatic collection cold traps (31).

7. The chiral compound separation and collection device according to claim 1, characterized in that: The pretreatment mechanism (1) comprises a filtering component (11) and a fractionation component (12), wherein the filtering component (11) is used to filter the sample liquid to obtain a pure sample liquid, and the fractionation component (12) is used to preliminarily remove non-target compounds in the pure sample liquid to obtain a pretreated sample liquid.

8. The chiral compound separation and collection device according to claim 7, characterized in that: The filter assembly (11) comprises a filter cartridge (51), a piston (52), a collecting cartridge (53), a driving member (54) and a blocking cartridge (55); the filter cartridge (51) is fixedly arranged in the collecting cartridge (53); the collecting cartridge (53) is used to collect the pure sample liquid filtered out by the filter cartridge (51); the driving member (54) is fixedly installed on the top of the collecting cartridge (53); the driving member (54) is transmission-connected to the piston (52); the driving member (54) is used to drive the piston (52) to move up and down in the filter cartridge (51); The filter cylinder (51) is provided with a plurality of evenly distributed filter holes (511) on its wall. When the piston (52) moves downward, the piston (52) filters the sample liquid out from the filter holes (511) of the filter cylinder (51). The blocking cylinder (55) is slidably arranged in the collecting cylinder (53). A ventilation area (56) is arranged between the piston (52) and the blocking cylinder (55). When the piston (52) and the blocking cylinder (55) move upward synchronously, the blocking cylinder (55) blocks the filter holes (511) located in the blocking cylinder (55).

9. The chiral compound separation and collection device according to claim 8, characterized in that: A transmission member (61) is provided at the bottom of the piston (52), and the transmission member (61) passes through the filter cartridge (51). A one-way limiting member (62) is provided at the bottom of the blocking cartridge (55). When the transmission member (61) is engaged with the one-way limiting member (62), the blocking cartridge (55) moves upward synchronously with the filter cartridge (51).

10. The chiral compound separation and collection device according to claim 9, characterized in that: The one-way limiting member (62) comprises a first elastic ring (621), the first elastic ring (621) is fixedly connected to the inner wall of the blocking tube (55), the transmission member (61) comprises a transmission frame (611), the transmission frame (611) is fixedly connected to the piston (52), a second elastic ring (612) is arranged on the transmission frame (611), and limiting rings (531) are arranged at both upper and lower ends of the collecting tube (53). When the blocking tube (55) abuts against the limiting ring (531), the piston (52) drives the second elastic ring (612) through the transmission frame (611) to squeeze and pass through the first elastic ring (621), thereby realizing the engagement or disengagement of the second elastic ring (612) and the first elastic ring (621).

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