Method for reducing hardness of coal gangue by using microorganisms

Abstract

The invention discloses a method for reducing the hardness of coal gangue by using microorganisms, and particularly, the method comprises the following steps: activating the microorganisms, and carrying out enlarged culture to obtain a bacterial suspension; mixing the bacterial suspension or a culture solution obtained by separating thalli of the bacterial suspension with coal gangue; the microorganisms comprise ardisia japonica phyllobacterium and / or floating mould, the preservation number of the ardisia japonica phyllobacterium is CGMCC (China General Microbiological Culture Collection Center) No.30592, and the preservation number of the floating mould is CGMCC No.30591. According to the method, the hardness of the coal gangue can be remarkably reduced, and the energy consumption in subsequent crushing utilization of the coal gangue is reduced.

Description

Technical Field

[0001] The invention belongs to the field of industrial solid waste disposal, and specifically relates to a method for reducing the hardness of coal gangue by utilizing microorganisms. Background Art

[0002] Coal gangue is one of the main wastes generated during coal mining and coal processing, and its main components include dry ash, kaolin, hydromica, bauxite, etc. With the increasing attention paid to global environmental protection and sustainable utilization of resources, the comprehensive utilization of coal gangue has become increasingly important.

[0003] At present, the general treatment method for coal gangue is landfill. Under natural conditions, the weathering process will release heavy metal elements in the coal gangue to the surrounding area, causing secondary pollution. The sustainable use of coal gangue is mainly reflected in road paving, building material manufacturing, fuel power generation and handicraft production. The common characteristics of these solutions are large project investment and high production costs, which seriously restrict the resource utilization of coal gangue.

[0004] Patent document 1 (A method for accelerating the transformation of waste rock into soil using coal gangue, publication number CN113441533A) discloses a method for accelerating the transformation of waste rock into soil using coal gangue. In this process, the heat generated during the combustion of coal gangue is used to heat the ore, which is then cooled and crushed by water to achieve the purpose of accelerating the transformation of waste rock into soil, while also solving the damage to the ecology caused by the accumulation of coal gangue.

[0005] Patent document 2 (A strain of Penicillium oxalate that decomposes coal gangue, publication number CN110951626A) discloses a method of using microorganisms to accelerate the release of nutrients from coal gangue. This method isolates a strain of Penicillium oxalate that decomposes coal gangue from a mining area. The strain has strong resistance to heavy metals, and uses its own small size and fast reproduction to use hyphae to obtain nutrients inside the coal gangue, thereby promoting the degradation of the coal gangue.

[0006] Patent Document 1 uses coal gangue combustion to release heat, which releases a large amount of pollutants in the process and easily causes secondary pollution.

[0007] The method in Patent Document 2 is relatively simple, but the gangue must first be crushed and mixed with soil, and the gangue blocks cannot be disposed of. There is still a limitation of high crushing energy consumption, and it is difficult to promote and use.

[0008] In addition, the technical routes of using coal gangue as raw material for cement production and roadbed materials all require the coal gangue to be crushed and ground for further processing. However, due to the high equipment requirements and high production costs of existing technologies, coal gangue products lack competitiveness in the market and are easily replaced. Therefore, there is an urgent need for a technology that can significantly reduce the hardness of coal gangue, so as to further reduce the cost of coal gangue crushing and improve the competitiveness of coal gangue resource products. Summary of the invention

[0009] In order to solve the deficiencies in the prior art, the purpose of the present invention is to provide a method that does not require the gangue to be crushed and ground and can significantly reduce the hardness of the gangue. To achieve this purpose, the present invention adopts the following technical solutions:

[0010] In a first aspect, the present invention provides a method for reducing the hardness of coal gangue using microorganisms, comprising:

[0011] Activate and expand the culture of microorganisms to obtain a bacterial suspension;

[0012] Mixing the bacterial suspension or the culture solution obtained by separating the bacterial bodies of the bacterial suspension with coal gangue for disposal;

[0013] The microorganisms include Bacillus sargentii and / or Planctomyces pyrenoidosa.

[0014] The deposit number of Bacillus sartorius is CGMCC No.30592, and the deposit number of Planctomyces oxysporum is CGMCC No.30591.

[0015] As a preferred embodiment, the microorganisms are Bacillus bisporus and Planctomyces pyrifer. More preferably, the number ratio of Bacillus bisporus and Planctomyces pyrifer is (0.5-2):1.

[0016] As a preferred embodiment, the ratio of the number of Bacillus sargentii and Planctomyces oxysporum is 1:1.

[0017] As an embodiment, the expanded culture is to culture the microorganism to the logarithmic growth phase.

[0018] According to a specific embodiment of the present invention, the strain concentration in the bacterial suspension is greater than 5.0×10 6 Pieces / mL.

[0019] As a preferred embodiment, the strain concentration in the bacterial suspension is 6.0×10 6 Pieces / mL.

[0020] As an embodiment, the method comprises: activating and expanding the microorganisms to obtain a bacterial suspension, isolating the bacterial bodies in the bacterial suspension, and mixing the obtained culture solution with coal gangue for treatment.

[0021] As an embodiment, the method comprises: activating and expanding the microorganisms to obtain a bacterial suspension, inoculating the bacterial strains obtained after separating the bacterial suspension into coal gangue, and then adding liquid culture medium for treatment.

[0022] As an implementation mode, when the bacterial strain obtained after separation of the bacterial suspension is inoculated into the coal gangue, 0.1% to 10% of the bacterial strain is added relative to the mass of the coal gangue.

[0023] As an implementation mode, the liquid-to-solid mass ratio of the bacterial suspension to the coal gangue is 1:1 to 5:1.

[0024] As an implementation mode, the liquid-to-solid mass ratio of the bacterial suspension to the coal gangue is 5:1.

[0025] In the present invention, the culture medium for expanding the culture of the microorganism is a liquid culture medium. According to a specific embodiment of the present invention, the liquid culture medium comprises one or more of peptone, beef extract, yeast extract, glucose, sodium chloride, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, magnesium sulfate, and ferrous sulfate.

[0026] As an implementation method, the process parameters of the treatment include: a treatment temperature of 18 to 35°C, a pH of 4 to 8, a dissolved oxygen concentration of 0 to 0.6 mmol / L, and a treatment time of no less than 10 days.

[0027] As an implementation method, the treatment process parameters include: treatment temperature of 18-35°C, pH of 4-8, and treatment time of not less than 30 days.

[0028] As a preferred embodiment, the treatment process parameters are: treatment temperature is 30°C, pH is 6.8, dissolved oxygen concentration is 0.3 mmol / L, and treatment time is 45 days.

[0029] In the present invention, the volume equivalent particle size of the coal gangue is not less than 20 mm.

[0030] In a second aspect, the present invention provides a microbial agent comprising Bacillus argenteus and / or Planctomyces pyogenes, wherein the preservation number of Bacillus argenteus is CGMCC No.30592, and the preservation number of Planctomyces pyogenes is CGMCC No.30591.

[0031] As a preferred embodiment, the microbial agent comprises Bacillus sargentii and Planctomyces pyrifer.

[0032] According to a specific embodiment of the present invention, the ratio of the number of Bacillus argenteus to that of Planctomyces is (0.5-2):1.

[0033] As a preferred embodiment, the ratio of the number of Bacillus sargentii and Planctomyces oxysporum is 1:1.

[0034] In a third aspect, the present invention provides the use of the microbial agent described in the second aspect of the present invention in reducing the hardness of coal gangue.

[0035] Beneficial effects:

[0036] The present invention provides a method for reducing the hardness of coal gangue by using microorganisms. The microorganisms selected in the present invention are environmentally friendly and safe, and have a wide range of sources. The method of the present invention can significantly reduce the hardness of coal gangue and reduce the energy consumption in its subsequent crushing and utilization. The method of the present invention has a certain chelation effect on heavy metals in coal gangue, reduces its activity, and reduces secondary pollution. The method of the present invention can also be used for the disposal of slag separated from other types of mines, such as manganese ore waste slag. The method of the present invention has a flexible process, and the most suitable method can be selected for disposal according to the actual situation. BRIEF DESCRIPTION OF THE DRAWINGS

[0037] Figure 1 This is the effect of treatment with Bacillus bisporus and / or Planctomyces oxysporum on the hardness of coal gangue.

[0038] Deposit of microorganisms for patent procedures:

[0039] 1. Leaf Bacillus argenteus of the present invention

[0040] Deposit date: May 10, 2024;

[0041] Depository: China General Microbiological Culture Collection Center (CGMCC);

[0042] Deposit number: CGMCC No.30592;

[0043] Classification and nomenclature: Phyllobacterium myrsinacearum;

[0044] Address of the depository: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing.

[0045] 2. Planctomyces of the present invention

[0046] Deposit date: May 10, 2024;

[0047] Depository: China General Microbiological Culture Collection Center (CGMCC);

[0048] Deposit number: CGMCC No.30591;

[0049] Classification and nomenclature: Planctomyces sp.;

[0050] Address of the depository: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing. DETAILED DESCRIPTION

[0051] Before further describing the specific embodiments of the present invention, it should be understood that the scope of protection of the present invention is not limited to the specific embodiments described below; it should also be understood that the terms used in the examples of the present invention are for describing the specific embodiments rather than for limiting the scope of protection of the present invention.

[0052] When the embodiments give numerical ranges, it should be understood that, unless otherwise specified in the present invention, both endpoints of each numerical range and any numerical value between the two endpoints can be selected. Unless otherwise defined, all technical and scientific terms used in the present invention have the same meaning as those generally understood by those skilled in the art. In addition to the specific methods, equipment, and materials used in the embodiments, according to the grasp of the prior art by those skilled in the art and the record of the present invention, any methods, equipment, and materials of the prior art similar or equivalent to the methods, equipment, and materials described in the embodiments of the present invention can also be used to realize the present invention.

[0053] Unless otherwise stated, the experimental methods, detection methods, and preparation methods disclosed in the present invention all adopt conventional techniques in the technical field.

[0054] In this specification, the numerical range expressed using "a numerical value A to a numerical value B" means a range including the endpoints numerical values ​​A and B.

[0055] In this specification, the unit names used are all international standard unit names, and unless otherwise stated, the "%" used indicates the volume percentage.

[0056] Example 1

[0057] A mixed treatment of Phyllobacterium Myrsinacearum (CGMCC No. 30592) and Planctomyces sp. (CGMCC No. 30591) was used, and the ratio of Phyllobacterium Myrsinacearum to Planctomyces sp. was 1:1. After the strain was activated, a liquid culture medium (10 g of peptone, 5 g of yeast extract, 1 g of glucose, 1 g of magnesium sulfate, 1 L of distilled water, and pH adjusted to 6.8 using HCl and NaOH) was used for 40 hours to an initial strain concentration of about 6*10 6 / mL to obtain a bacterial suspension.

[0058] The bacterial suspension was mixed with sterilized coal gangue in a mass ratio of 5:1, the temperature was maintained at 30°C, the dissolved oxygen concentration was 0.3 mmol / L, and after 45 days of treatment, the Mohs hardness of the coal gangue decreased from 6 to 4.2.

[0059] In the present invention, the test method for the hardness of coal gangue is: use a Mohs hardness pen to draw lines on the flat part of the coal gangue from small to large Mohs hardness, observe the scratches, when no scratches are left or the scratches are not obvious, the hardness of the coal gangue at this time is less than the corresponding indication of the hardness pen used, when obvious scratches are left, the hardness is the hardness of the coal gangue at this time.

[0060] Example 2

[0061] Phyllobacterium Myrsinacearum (CGMCC No. 30592) was used for treatment. After the strain was activated, liquid culture medium (10 g peptone, 5 g yeast extract, 1 g glucose, 1 g magnesium sulfate, 1 L distilled water, pH adjusted to 6.8 using HCl and NaOH) was used for 40 h, until the initial strain concentration was about 6*10 6 / mL to obtain a bacterial suspension.

[0062] The bacterial suspension was mixed with sterilized coal gangue in a mass ratio of 5:1, the temperature was maintained at 30°C, the dissolved oxygen concentration was 0.3 mmol / L, and after 45 days of treatment, the Mohs hardness of the coal gangue decreased from 6 to 4.9.

[0063] Example 3

[0064] Planctomyces sp. (CGMCC No. 30591) was used for treatment. After the strain was activated, liquid culture medium (10 g peptone, 5 g yeast extract, 1 g glucose, 1 g magnesium sulfate, 1 L distilled water, pH adjusted to 6.8 using HCl and NaOH) was used for 40 h, until the initial strain concentration was about 6*10 6 / mL to obtain a bacterial suspension.

[0065] The bacterial suspension was mixed with sterilized coal gangue in a mass ratio of 5:1, the temperature was maintained at 30°C, the dissolved oxygen concentration was 0.3 mmol / L, and after 45 days of treatment, the Mohs hardness of the coal gangue decreased from 6 to 5.2.

[0066] The results of the effects of treatment with Bacillus sargentii and / or Planctomyces on the hardness of coal gangue are shown in Figure 1 .

[0067] The above description of the embodiments is to facilitate the understanding and use of the invention by those skilled in the art. It is obvious that those skilled in the art can easily make various modifications to these embodiments and apply the general principles described herein to other embodiments without creative work. Therefore, the present invention is not limited to the above embodiments, and improvements and modifications made by those skilled in the art based on the disclosure of the present invention without departing from the scope of the present invention should be within the scope of protection of the present invention.

Claims

1. A method for reducing the hardness of coal gangue using microorganisms, comprising: Activate and expand the culture of microorganisms to obtain a bacterial suspension; Mixing the bacterial suspension or the culture solution obtained by separating the bacterial bodies of the bacterial suspension with coal gangue for disposal; The microorganisms include Bacillus sargentii and / or Planctomyces pyrenoidosa. The deposit number of Bacillus sartorius is CGMCC No.30592, and the deposit number of Planctomyces oxysporum is CGMCC No.30591.

2. The method according to claim 1, in, The microorganisms are Bacillus bisporus and Planctomyces pyogenes. Preferably, the quantity ratio of Bacillus bisporus and Planctomyces pyogenes is (0.5-2):

1.

3. The method according to claim 1, in, The strain concentration in the bacterial suspension is greater than 5.0×10 6 Pieces / mL.

4. The method according to claim 3, in, The strain concentration in the bacterial suspension is 6.0×10 6 Pieces / mL.

5. The method according to claim 1, in, The liquid-solid mass ratio of the bacterial suspension to the coal gangue is 1:1 to 5:

1.

6. The method according to claim 5, in, The liquid-solid mass ratio of the bacterial suspension to the coal gangue is 5:

1.

7. The method according to any one of claims 1 to 6, in, The treatment process parameters include: treatment temperature of 18-35°C, pH of 4-8, dissolved oxygen concentration of 0-0.6 mmol / L, and treatment time of no less than 10 days.

8. A microbial agent comprising Bacillus sargentii and / or Planctomyces pyrenoidosa, in, The deposit number of Bacillus sartorius is CGMCC No.30592, and the deposit number of Planctomyces oxysporum is CGMCC No.30591.

9. The microbial agent according to claim 8, comprising Bacillus bisporus and Planctomyces bisporus, preferably, the quantity ratio of Bacillus bisporus to Planctomyces bisporus is (0.5-2):

1.

10. Use of the microbial agent according to claim 8 or 9 in reducing the hardness of coal gangue.

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