Method for improving anaerobic ammonia oxidation performance based on stimulation of cell envelope stress and application

Abstract

The invention discloses a method for improving anaerobic ammonia oxidation performance based on stimulation of cell envelope stress and application, and relates to the technical field of water treatment. According to the method, an enveloping reagent is added in a pulse feeding mode to trigger the enveloping stress of anaerobic ammonium oxidation bacteria, and due to the fact that continuous adding of the enveloping reagent can cause serious sludge floating, the pulse feeding mode can have time to balance the lipid removal rate of the enveloping reagent and the lipid synthesis rate of anaerobic ammonium oxidation cells. Finally, the purpose of improving the anaerobic ammonia oxidation performance is achieved, and the denitrification efficiency is improved. The method is suitable for a sewage treatment facility which cannot provide sufficient uniform mixing strength or has a dead zone, mechanical stirring is partially replaced by chemical stimulation, the situation that the anaerobic ammonia oxidation process is low in efficiency and unstable is avoided, and the method can be widely popularized.

Description

Technical Field

[0001] The present invention relates to the technical field of water treatment, and in particular to a method and application of improving anaerobic ammonia oxidation performance based on stimulating cell envelope stress. Background Art

[0002] Anaerobic ammonium oxidation is widely regarded as a promising biological denitrification method with good treatment performance and cost-effectiveness (Mishra et al, 2022). However, the presence of insufficiently mixed areas / dead zones in sewage treatment facilities leads to reduced mass transfer, which limits the denitrification efficiency of anaerobic ammonium oxidizing bacteria. By triggering the cell's membrane stress, the nitrogen metabolic pathway of anaerobic ammonium oxidizing bacteria can be strengthened, thereby partially compensating for the problems caused by insufficient mixing in the reactor. Membrane stress is a bacterial stress state caused by external pressure or adverse environmental conditions, which can affect the intracellular material transfer and metabolic capacity, and regulate cell growth and metabolic activity (Mitchell & Silhavy, 2019). How to enable anaerobic ammonium oxidizing bacteria to better adapt to and utilize limited substrates and effectively improve nitrogen removal efficiency under conditions of insufficient sewage mixing is a technical problem that needs to be solved urgently in this field.

[0003] In view of this, the present invention is proposed. Summary of the invention

[0004] The purpose of the present invention is to provide a method and application for improving anaerobic ammonia oxidation performance based on stimulating cell envelope stress, so as to solve the above technical problems.

[0005] The present invention is achieved in that:

[0006] In a first aspect, an embodiment of the present invention provides a method for improving anaerobic ammonium oxidation performance based on stimulating cell envelope stress, comprising: adding an envelope reagent to sewage in a pulse mode to carry out an anaerobic ammonium oxidation reaction; wherein the envelope reagent is selected from at least one of β-cyclodextrin, procaine and dipyridamole.

[0007] In a second aspect, an embodiment of the present invention provides an application of the aforementioned method in anaerobic ammonium oxidation treatment of wastewater.

[0008] The present invention has the following beneficial effects:

[0009] A method for improving anaerobic ammonium oxidation performance based on stimulating cell envelope stress provided in an embodiment of the present invention comprises: adding an envelope reagent to sewage in a pulse mode to carry out an anaerobic ammonium oxidation reaction; using the envelope reagent to trigger the envelope stress of anaerobic ammonium oxidizing bacteria; and the envelope reagent is added in a pulse mode, which can have time to balance the lipid removal rate of β-cyclodextrin and the lipid synthesis rate of anaerobic ammonium oxidizing cells, and finally achieve the purpose of improving anaerobic ammonium oxidation performance; this method can enhance the activity of anaerobic ammonium oxidizing bacteria and improve denitrification efficiency; this method is suitable for when sewage treatment facilities cannot provide sufficient mixing intensity or there is a dead zone, and chemical stimulation is used to partially replace mechanical stirring to avoid inefficiency and instability of the anaerobic ammonium oxidation process, and can be widely promoted. BRIEF DESCRIPTION OF THE DRAWINGS

[0010] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required for use in the embodiments are briefly introduced below. It should be understood that the following drawings only show certain embodiments of the present invention and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other related drawings can be obtained based on these drawings without creative work.

[0011] Figure 1 The arrow indicates the addition of 600 μM coating reagent once on that day.

[0012] Figure 2 The nitrogen concentration of the inlet and outlet water of the anaerobic ammonium oxidation reaction pool: the first 20 days are the treatment effects without adding the coating reagent, and after 20 days are the treatment effects of adding the coating reagent in a pulsed manner;

[0013] Figure 3 A schematic diagram of a device for adding coated reagents to improve the performance of anaerobic ammonium oxidation;

[0014] Figure 4 It is a schematic diagram of the denitrification effect test results of Examples 1-3 and Comparative Examples 1-2.

[0015] Icons: 1-water inlet regulating tank; 2-electric gate; 3-first water pump; 4-coating reagent dosing device; 5-magnetic stirring water tank; 6-magnetic stirring rotor; 7-touch screen; 8-coating reagent adding tank; 9-second water pump; 10-anaerobic ammonia oxidation reaction tank; 11-mechanical stirrer. DETAILED DESCRIPTION

[0016] In order to make the purpose, technical scheme and advantages of the embodiments of the present invention clearer, the technical scheme in the embodiments of the present invention will be described clearly and completely below. If the specific conditions are not specified in the embodiments, they are carried out according to conventional conditions or conditions recommended by the manufacturer. If the manufacturer of the reagents or instruments used is not specified, they are all conventional products that can be purchased commercially.

[0017] The present invention enhances the permeability of the biofilm by adding a coating agent, causing a coating stress reaction, thereby achieving that under the condition of insufficient sewage mixing, the anaerobic ammonia oxidizing bacteria can still better adapt to and utilize limited substrates, effectively improving the nitrogen removal efficiency.

[0018] In a first aspect, the present invention provides a method for improving anaerobic ammonium oxidation performance based on stimulating cell envelope stress, comprising: adding an envelope reagent to sewage in a pulse mode to carry out an anaerobic ammonium oxidation reaction; wherein the envelope reagent is selected from at least one of β-cyclodextrin, procaine and dipyridamole.

[0019] It should be noted that the coating agent is an amphiphilic substance, which can enhance the nitrogen metabolism pathway of anaerobic ammonia-oxidizing bacteria by triggering the cell's coating stress, thereby partially compensating for the problems caused by insufficient mixing in the reactor.

[0020] Among them, β-cyclodextrin is an organic compound that exists in the form of white powder or crystals. Because it belongs to the heptasaccharide substance that can be extracted from glucose, it is easy to produce and inexpensive. β-cyclodextrin is soluble in water and is not hygroscopic, but it is easy to form stable hydrates. In addition, β-cyclodextrin is also known for its low toxicity (LD50 <12500mg kg -1 , acute oral dose in rats and mice) and biodegradability, so it is classified as an environmentally friendly chemical and is suitable for engineering applications.

[0021] Procaine exists as white crystals or crystalline powder, is easily soluble in water, has low toxicity, and can disrupt cell membrane dynamics by blocking ion channel proteins.

[0022] Dipyridamole is a yellow crystalline powder that is commonly used as a phosphodiesterase inhibitor, indicating that it can interact with cell membranes.

[0023] In an alternative embodiment of the present invention, the coating agent comprises β-cyclodextrin.

[0024] In an optional embodiment of the present invention, the pulse mode includes adding the coating agent at least once every 24 hours.

[0025] It should be noted that the pulse mode is a periodic working and stopping mode, that is, the dosing is stopped when the anaerobic ammonium oxidation effect continues to improve, and the dosing is continued until the treatment effect becomes worse; the dosing frequency can be dynamically adjusted according to the anaerobic ammonium oxidation denitrification effect. In addition, the control of the dosing frequency of the coating agent is to avoid the large-scale floating of the anaerobic ammonium oxidation particles on the one hand, and to test whether a good treatment effect can be maintained when the dosing is not intermittent on the other hand.

[0026] Specifically, the interval time for adding the coating agent in pulse mode is at least 24 hours, for example, the interval time is selected from 1 day, 2 days, 3 days, 4 days, 5 days, 6 days, 8 days, 10 days, 15 days, 18 days, 20 days, etc., or other reasonable time; in other embodiments of the present invention, the interval time setting can be reasonably adjusted according to the actual amount of sewage treated and the concentration of the coating agent used.

[0027] In an optional embodiment of the present invention, the coating agent is dissolved before being added to the sewage, wherein the concentration of the coating agent solution is 580 μM-610 μM, preferably 600 μM.

[0028] Specifically, a central controller is used to achieve precise control of the coating reagent, and during the dissolution of the coating reagent, the nitrogen concentration in the water is quantitatively analyzed to optimize and determine the appropriate dosage and frequency of β-cyclodextrin. The dosage of β-cyclodextrin powder is aimed at activating the stress response of the anaerobic ammonium oxidation cell envelope, and this purpose can be achieved when the β-cyclodextrin concentration of the anaerobic ammonium oxidation reaction tank 10 reaches 600 μM.

[0029] It should be noted that the coating reagent can be stirred reasonably according to actual needs during the dissolution process to ensure that the coating reagent is fully dissolved in water; the determination of β-cyclodextrin concentration requires manual sampling. The specific dosage of β-cyclodextrin is related to the amount of sewage to be treated, and can be reasonably adjusted according to actual conditions.

[0030] In an optional embodiment of the present invention, the denitrification efficiency of the sewage after adding the coating agent is 67.4%±4.8%.

[0031] It should be noted that the calculation of denitrification efficiency requires manual sampling and quantitative analysis of the nitrogen concentration in the water. Secondly, it can also optimize and determine the appropriate dosage and frequency of coating reagents.

[0032] In an optional embodiment of the present invention, the cycle of the sequencing batch operation is 10h-13h, and the exchange rate is 50%; further, the cycle of the sequencing batch operation is 12h.

[0033] In an optional embodiment of the present invention, anaerobic ammonia oxidation is carried out in a sequencing batch operation mode, and the sequencing batch operation includes a water intake stage, a reaction stage, a sedimentation stage and a drainage stage; wherein the water intake stage lasts for 3 min to 6 min, the reaction stage lasts for 11 h to 12 h, the sedimentation stage lasts for 18 min to 22 min, and the drainage stage lasts for 4 min to 6 min.

[0034] It should be noted that by precisely controlling the time and conditions of each stage, we can ensure that each step can achieve the best results; the fine control of each stage makes the effluent quality more stable and reliable; by adjusting the time allocation of each stage, it can better adapt to different changes in influent water quality and water volume; by optimizing the operating cycle, unnecessary energy consumption and equipment operating time can be reduced, thereby reducing operating costs.

[0035] In addition, the anaerobic ammonium oxidation reaction is carried out in a sequencing batch mode, which has the following characteristics: the ideal plug flow process increases the driving force of the biochemical reaction, improves the efficiency, and has a good purification effect; the operation effect is stable; there is retained treated water in the reaction tank, which has a dilution and buffering effect on the sewage, and effectively resists the impact of water volume and organic pollutants; each process in the process can be adjusted according to water quality and water volume, and the operation is flexible; in addition, the sequencing batch operation has relatively few treatment equipment, simple structure, and is easy to operate and maintain; the process flow is simple, the sequencing batch operation occupies a small area, and the cost is low.

[0036] Among them, the water inlet stage is to introduce sewage into the reactor to prepare for subsequent treatment; the reaction stage is to remove inorganic nitrogen in the water through anaerobic ammonia oxidation reaction; the sedimentation stage is to use gravity to separate the sludge and water for subsequent drainage operations; the drainage stage is to discharge the treated water to complete a cycle of treatment.

[0037] In an optional embodiment of the present invention, the sequencing batch operation mode also includes a static stage after the drainage stage, and its main function includes separating the sediment and muddy water to improve the effluent water quality and sludge concentration, which is beneficial to subsequent treatment or resource utilization and improves the treatment efficiency and stability of the entire treatment system. In this stage, the suspended solids in the mixed liquid settle under the action of gravity, thereby achieving muddy water separation.

[0038] Furthermore, the time for the water inlet stage was 5 min, the time for the reaction stage was 11.5 h, the time for the sedimentation stage was 20 min and the time for the drainage stage was 5 min.

[0039] In an optional embodiment of the present invention, stirring the solution in the anaerobic ammonium oxidation reaction tank 10 is beneficial to mixing the solution within a certain range, and the stirring speed and time can be set according to the actual water quality of the inlet and outlet water.

[0040] In an optional embodiment of the present invention, the device for adding a coated reagent to improve the anaerobic ammonium oxidation performance includes a water inlet regulating tank 1, a coated reagent doser 4 and an anaerobic ammonium oxidation reaction tank 10. For details, please refer to Figure 3 .

[0041] Among them, the water outlet of the water inlet regulating tank 1 is connected with the water inlet of the coating reagent dosing device 4, and the electric gate 2 and the first water pump 3 are connected at the water outlet of the water inlet regulating tank 1; the sewage first enters the water inlet regulating tank 1, and the collected sewage is pumped into the coating reagent dosing device 4 through the electric gate 2 and the first water pump 3.

[0042] The coating reagent dosing device 4 is provided with a magnetic stirring water tank 5, a touch display screen 7, a central controller and a coating reagent adding tank 8, and a magnetic stirring rotor 6 is also provided inside the magnetic stirring water tank 5; the coating reagent dosing device 4 releases the powder of the coating reagent and fully dissolves it in the sewage through magnetic stirring, and the sewage is pumped into the anaerobic ammonia oxidation reaction tank 10 through the second water pump 9.

[0043] Specifically, the touch screen 7 is connected to the central controller, and can accurately control the weight and frequency of sample addition. During operation, the powder mass of the coating reagent to be added is pre-set through the touch screen 7, and the central controller controls the coating reagent sample slot 8 to open the slot to release the coating reagent powder. Then, the magnetic stirring rotor 6 in the magnetic stirring water tank 5 rotates to quickly mix and fully dissolve the coating reagent.

[0044] It should be noted that the embodiment of the present invention does not particularly limit the specifications and models of the first water pump 3 and the second water pump 9. According to actual needs, an ordinary water pump with a single structure and function, or an integrated multifunctional water pump can be selected.

[0045] The water outlet of the coating reagent dosing device 4 is connected to the water inlet of the anaerobic ammonium oxidation reaction tank 10, and the material in the coating reagent dosing device 4 is pumped into the anaerobic ammonium oxidation reaction tank 10 for reaction through the second water pump 9. The anaerobic ammonium oxidation reaction tank 10 is connected to a mechanical stirrer 11; the mechanical stirrer 11 can provide mixing for a certain range of liquids, and the speed and stirring time of the mechanical stirrer 11 can be set according to the water quality of the inlet and outlet water. When the mechanical stirrer 11 is turned on, the anaerobic ammonium oxidation reaction is carried out for a certain period of time to remove ammonia nitrogen and nitrite nitrogen in the water, and the sewage after effective denitrification is discharged through the electric gate 2.

[0046] It should be noted that the anaerobic ammonium oxidation reaction tank 10 is operated in a batch mode, with each cycle being 12 hours, an exchange rate of 50%, and a hydraulic retention time of approximately 24 hours; this setting can ensure that the anaerobic ammonium oxidation bacteria are in full contact with the sewage, thereby removing ammonia nitrogen and nitrite nitrogen in the sewage to the greatest extent.

[0047] In summary, the working principle of the device for adding coated reagents to improve the performance of anaerobic ammonia oxidation is as follows:

[0048] The sewage is first collected in the water inlet regulating tank 1, and enters the coating reagent dosing device 4 through the electric gate 2 and the first water pump 3. According to the dosing parameters set on the touch screen 7, the dosing quality and frequency of the coating reagent powder are regulated, and the coating reagent powder is fully dissolved in the magnetic stirring water tank 5. Subsequently, the sewage is pumped into the anaerobic ammonia oxidation reaction tank 10 via the second water pump 9. The anaerobic ammonia oxidizing bacteria are subjected to the coating stress caused by the coating reagent, and their nitrogen metabolism pathway is stimulated and strengthened, thereby improving the biological denitrification efficiency. The exchange rate is 50%, and the hydraulic retention time is about 24 hours, which can ensure that the anaerobic ammonia oxidizing bacteria are fully in contact with the sewage and remove ammonia nitrogen and nitrite nitrogen in the sewage to the greatest extent.

[0049] In a second aspect, an embodiment of the present invention provides an application of the aforementioned method in anaerobic ammonium oxidation treatment of wastewater.

[0050] The features and performance of the present invention are further described in detail below in conjunction with the embodiments.

[0051] Example 1

[0052] This embodiment provides a method for improving anaerobic ammonium oxidation performance based on stimulating cell envelope stress, which comprises the following steps:

[0053] (1) The sewage collected in the water regulating tank 1 is the mainstream (low-concentration) urban sewage after partial nitrification, and its characteristics are: the ammonia nitrogen concentration is 50 mg L -1 , nitrite nitrogen concentration is 66 mg L -1 , pH range 7-8, dissolved oxygen less than 0.1 mg L -1 , the dosage is 1L.

[0054] (2) The coating agent is β-cyclodextrin, and the coating agent doser 4 adds β-cyclodextrin powder to the magnetic stirring water tank 5 in a pulse mode to control the concentration of β-cyclodextrin in the water to be 600 μM. During this period, the magnetic stirring is turned on to ensure that the β-cyclodextrin is fully dissolved in the water.

[0055] The dosage of β-cyclodextrin powder in this embodiment accounts for 0.068% of the amount of sewage treatment.

[0056] The specific dosing frequency is as follows Figure 1 Shown: The intervals from left to right are 4, 4, 6, 8, 4, 18, 6, 5, 6, 4, 5, 8, 6, 4 days.

[0057] (3) The anaerobic ammonium oxidation reaction tank 10 is operated in a batch mode, with each cycle lasting 12 hours, including a water inlet phase of 5 minutes, a reaction phase of 11.5 hours, a sedimentation phase of 20 minutes, and a drainage phase of 5 minutes. The exchange rate is 50%, and the hydraulic retention time is about 24 hours to ensure that the anaerobic ammonium oxidation bacteria are in full contact with the sewage and remove ammonia nitrogen and nitrite nitrogen in the sewage to the greatest extent. The mechanical stirring speed is 40 rpm, and the sludge concentration VSS (volatile suspended solids) is 0.93 g / L -1 .

[0058] (4) After adding β-cyclodextrin to the water six times, the effluent ammonia nitrogen concentration dropped to 1.4 mg L -1 The nitrite nitrogen concentration in the effluent was 8.5 mg L -1 , the total inorganic nitrogen concentration was 33.4 mg L -1 The denitrification efficiency reached 72.4%. The effect of adding β-cyclodextrin on anaerobic ammonium oxidation during the entire experimental period is shown in Figure 2 shown.

[0059] Example 2

[0060] This embodiment provides a method for improving anaerobic ammonium oxidation performance based on stimulating cell envelope stress, which comprises the following steps:

[0061] (1) Add mainstream (low concentration) urban sewage to the serum bottle, which has the following characteristics: ammonia nitrogen concentration of 50 mg L -1 , nitrite nitrogen concentration is 66 mg L -1 , pH range 7-8, dissolved oxygen less than 0.1 mg L -1 , dosage is 100mL.

[0062] (2) The coating agent is β-cyclodextrin. The coating agent is directly dissolved in the influent water, fully stirred, and the concentration of β-cyclodextrin in the water is controlled to be 600 μM. The dosage of β-cyclodextrin powder in this embodiment accounts for 0.068% of the amount of sewage treated.

[0063] (3) After the serum bottle was placed on a shaker at 120 rpm and 30°C for 24 h, the concentrations of ammonia nitrogen and nitrite nitrogen in the water were determined. The remaining ammonia nitrogen concentration in the water was lower than the detection limit (<0.025 mg L -1 ), the remaining nitrite nitrogen concentration was 4.1 mg L -1 ±2.8mg L -1 The effect of adding β-cyclodextrin on anaerobic ammonium oxidation during the experimental period of this batch is shown in Figure 4 shown.

[0064] Example 3

[0065] This embodiment provides a method for improving anaerobic ammonia oxidation performance based on stimulating cell envelope stress, which differs from embodiment 2 only in that:

[0066] (2) The coating agent is procaine, and the concentration of procaine in water is controlled to be 600 μM. The dosage of procaine powder in this embodiment accounts for 0.013% of the amount of wastewater to be treated.

[0067] (3) The ammonia nitrogen concentration in the water of this embodiment is 10.8 mg L -1 ±0.6mg L -1 The remaining nitrite nitrogen concentration was 28.9 mg L -1 ±1.9mg L -1 The effect of adding procaine on anaerobic ammonium oxidation during the experimental period of this batch is shown in Figure 4 shown.

[0068] Example 4

[0069] This embodiment provides a method for improving anaerobic ammonia oxidation performance based on stimulating cell envelope stress, which differs from embodiment 2 only in that:

[0070] (2) The coating agent selected was dipyridamole, and the dipyridamole concentration in the water was controlled to be 600 μM. The dosage of the dipyridamole powder in this embodiment accounted for 0.030% of the amount used for treating the sewage.

[0071] (3) The residual ammonia nitrogen concentration in the water of this embodiment is 0.4 mg L -1 ±0.3mg L -1 , the remaining nitrite nitrogen concentration was 17.9 mg L -1 ±0.8mg L -1 The effect of adding dipyridamole on anaerobic ammonium oxidation during the experimental period of this batch is shown in Figure 4 shown.

[0072] It should be noted that Examples 2-4 are short-term batch experiments on coating agents, which are used to screen the optimal coating agent for stimulating cell coating stress to improve anaerobic ammonia oxidation performance; while Example 1 is a long-term experiment using β-cyclodextrin.

[0073] Comparative Example 1

[0074] This comparative example provides a method for improving anaerobic ammonia oxidation performance based on stimulating cell envelope stress, which differs from Example 1 only in that:

[0075] missing (2), i.e., no coating reagent was used;

[0076] (3) The sludge concentration VSS (volatile suspended solids) is 0.93 g L -1 .

[0077] (4) The ammonia nitrogen concentration in the effluent was 31.2 mg L -1 The nitrite nitrogen concentration in the effluent was 10.8 mg L -1 The average concentration of total inorganic nitrogen was 62.6 mg L -1 , the denitrification efficiency reached 49.0%.

[0078] Comparative Example 2

[0079] This comparative example provides a method for improving anaerobic ammonia oxidation performance based on stimulating cell envelope stress, which differs from Example 1 only in that:

[0080] (2) the coating reagent feeder 4 feeds β-cyclodextrin powder into the magnetic stirring water tank 5 in a continuous mode;

[0081] (3) The sludge concentration VSS (volatile suspended solids) is 0.93 g L -1 .

[0082] (4) The ammonia nitrogen concentration in the effluent was 48.6 mg L -1 ±1.1mg L -1 The nitrite nitrogen concentration in the effluent was 65.4 mg L -1 ±1.3mg L -1 The average concentration of total inorganic nitrogen was 114 mg L -1 ±1.3mg L -1 , the denitrification efficiency reached 1.7%.

[0083] Test Example 1

[0084] This test example tests the denitrification effect of Examples 1-3 and Comparative Examples 1-2. The test items include effluent ammonia nitrogen concentration, effluent nitrite nitrogen concentration, average total inorganic nitrogen concentration and denitrification efficiency. Among them, the test method for effluent ammonia nitrogen concentration is Nessler's reagent spectrophotometry; the test method for effluent nitrite nitrogen concentration is N-(1-naphthyl)-ethylenediamine spectrophotometry. The relevant results are summarized in Figure 4 .

[0085] The calculation formula of the average concentration of total inorganic nitrogen is as follows: average concentration of total inorganic nitrogen = effluent nitrite nitrogen + effluent ammonia nitrogen + effluent nitrate nitrogen;

[0086] The calculation formula for denitrification efficiency is as follows: Denitrification efficiency = (influent nitrite nitrogen + influent ammonia nitrogen - effluent total inorganic nitrogen) / (influent nitrite nitrogen + influent ammonia nitrogen).

[0087] Combination Figure 2 and Figure 4It can be seen that procaine has a negative impact on denitrification, reducing the removal of ammonia nitrogen and nitrite nitrogen by anaerobic ammonium oxidizing bacteria; dipyridamole has no statistical difference compared with the control group (i.e., the group without added coating reagent), and has no effect on denitrification; while β-cyclodextrin significantly promotes the removal of ammonia nitrogen and nitrite nitrogen by anaerobic ammonium oxidation reaction. The long-term verification test results of Example 1 show that the addition of 600 μM β-cyclodextrin in pulse mode significantly reduces the effluent nitrogen concentration, especially from 36 to 73 days, the effluent ammonia nitrogen concentration is as low as 1.4 mg L -1 -6.6mg L -1 During this period, the average concentration of total inorganic nitrogen in the effluent was 39.6 mg L -1 ±5.9mg L -1 , the denitrification efficiency reached 67.7%±4.5%; while the continuous addition of β-cyclodextrin would destroy the stability of sludge microorganisms and cause them to gradually die. It was observed that the sludge gradually turned black, and the anaerobic ammonia oxidation treatment effect was significantly reduced.

[0088] In summary, the method for improving the anaerobic ammonium oxidation performance provided by the embodiment of the present invention uses a pulse dosing mode to add β-cyclodextrin to trigger the envelope stress of anaerobic ammonium oxidizing bacteria, because the continuous addition of β-cyclodextrin will cause serious sludge floating, and the pulse dosing mode can have time to balance the lipid removal rate of β-cyclodextrin and the lipid synthesis rate of anaerobic ammonium oxidizing cells, ultimately achieving the purpose of improving the anaerobic ammonium oxidation performance and improving the denitrification efficiency. This method is suitable for sewage treatment facilities that cannot provide sufficient mixing intensity or there is a dead zone, and chemical stimulation is used to partially replace mechanical stirring to avoid inefficiency and instability in the anaerobic ammonium oxidation process.

[0089] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A method for improving anaerobic ammonium oxidation performance based on stimulating cell envelope stress, characterized in that: It includes: The coated reagent is added to the sewage in a pulse mode to carry out anaerobic ammonium oxidation reaction; The coating agent is selected from at least one of β-cyclodextrin, procaine and dipyridamole.

2. The method according to claim 1, characterized in that: The coating agent includes β-cyclodextrin.

3. The method according to claim 1, characterized in that The pulse mode includes adding the coating agent at least once every 24 hours.

4. The method according to claim 1, characterized in that: The coating agent also includes dissolving the coating agent before adding the coating agent into sewage.

5. The method according to claim 4, characterized in that The solution concentration of the coating agent is 580 μM-610 μM, preferably 600 μM.

6. The method according to claim 1, characterized in that The denitrification efficiency of the sewage after adding the coating agent is 67.4%±4.8%.

7. The method according to claim 1, characterized in that Anaerobic ammonium oxidation is carried out in a sequential batch operation mode, wherein the cycle of the sequential batch operation is 10h-13h and the exchange rate is 50%; Preferably, the cycle of the batch operation is 12 hours.

8. The method according to claim 1, characterized in that The sequencing batch operation includes a water intake stage, a reaction stage, a sedimentation stage and a drainage stage; Among them, the time for the water inlet stage is 3min-6min, the time for the reaction stage is 11h-12h, the time for the sedimentation stage is 18min-22min and the time for the drainage stage is 4min-6min.

9. The method according to claim 8, characterized in that In the sequencing batch operation, the time of the water inlet phase is 5 minutes, the time of the reaction phase is 11.5 hours, the time of the sedimentation phase is 20 minutes and the time of the drainage phase is 5 minutes.

10. Use of the method according to any one of claims 1 to 9 in anaerobic ammonia oxidation treatment of sewage.

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