Hand-held detector based on green fluorescent probe detection and detection method and application thereof

Abstract

The invention belongs to the technical field of microbiological detection, and relates to a handheld detector based on green fluorescent probe detection and a detection method and application thereof. The detector comprises a sample fusion assembly and a fluorescence collection assembly, the sample fusion assembly comprises a reagent tube, a swab and a handheld cap, and the top end of the swab is fixed in the handheld cap to form a sampling assembly; a squeezing-breaking assembly is mounted at the top of the sample tube, the test tube penetrates through the squeezing-breaking assembly, the squeezing-breaking assembly squeezes the test tube to squeeze and break the swab, and the swab falls into the test tube and is fused with the detection solution; a fluorescence collection assembly is arranged on the side of each sample fusion assembly, and after a sampled swab falls into a reagent tube, the swab can be subjected to fusion reaction with a detection solution, fluorescence excitation is carried out, a fluorescence sensor acquires change of a fluorescence signal, and the concentration of a pollutant to be detected is directly output after the change of the fluorescence signal is processed by a main control board. The concentration of the microbial toxins in the sample to be detected can be rapidly detected, the sample does not need to be sent to a professional institution for detection, and public users can perform detection by themselves.

Description

Technical Field

[0001] The present invention belongs to the technical field of microbial detection, and in particular relates to a handheld detector based on green fluorescent probe detection, a detection method and an application thereof. Background Art

[0002] In the screening of early colorectal cancer, there are a variety of cancer-related carcinogenic toxins in stool samples that can serve as important biomarkers. For example, Bacteroides fragilis toxin (BFT) secreted by Enterotoxigenic Bacteroides fragilis (ETBF) can induce intestinal inflammation and promote tumorigenesis; strains carrying the pks gene cluster in Escherichia coli can produce genotoxins (Genotoxins), which directly damage DNA and induce carcinogenesis; Cyclic peptide toxins (CDT) secreted by certain Escherichia coli and Campylobacter can cause cell cycle arrest and DNA damage; glucanosyltransferase toxins, produced by Clostridium difficile, are associated with intestinal epithelial cell lesions; and Shiga toxin (Stx), although usually associated with acute gastroenteritis, may also affect the development of intestinal tumors.

[0003] These toxins can be detected in feces through fluorescent probe technology, and through the separation and quantitative analysis of specific markers, they provide support for early screening of cancer. However, existing detection methods still face challenges such as interference from sample complexity, difficulty in probe development, and accuracy of quantitative detection, and are urgently needed to be further optimized to improve the reliability and sensitivity of early cancer screening.

[0004] The technologies currently used to detect cancer-related carcinogenic toxins in stool samples include PCR (polymerase chain reaction), ELISA (enzyme-linked immunosorbent assay), and mass spectrometry. PCR technology is used to detect toxin-producing bacteria by amplifying specific toxin genes, but it is limited to the gene level and cannot directly detect toxin proteins. ELISA is commonly used to detect protein toxins and can provide relatively accurate qualitative or quantitative information, but it is easily interfered by other substances in the feces. Mass spectrometry performs well in terms of toxin types and precise quantification, but due to the expensive equipment and complex sample processing, it is difficult to be widely used in screening.

[0005] These detection technologies face some problems in practical applications. First, the composition of fecal samples is complex, which may interfere with the detection signal, thus affecting sensitivity and accuracy. Secondly, the multiple toxins in feces require highly targeted detection methods, and existing methods make it difficult to detect multiple toxins in one step. In addition, certain high-precision tests such as mass spectrometry are not suitable for large-scale screening due to cost and operational complexity. Finally, existing technologies also have deficiencies in the quantitative detection of toxin concentrations and the standardization of results. These problems limit the widespread application of these technologies in early cancer screening. Improving these detection technologies to enhance their high-throughput, rapid, and low-cost detection capabilities in complex samples will contribute to the effective screening of early cancer.

[0006] Fluorescent probes have significant advantages in early cancer screening. First, their high sensitivity can amplify the detection signal and capture low concentrations of target toxins, which is particularly suitable for early detection. In addition, the high specificity of fluorescent probes enables them to accurately bind to specific toxins, reduce nonspecific interference, and improve detection accuracy. The detection process is also relatively fast, without the need for complex sample processing, and is suitable for screening that requires real-time results. Fluorescent signals are easy to observe or read, making it easy to quickly obtain visual results in clinical practice. Moreover, because the equipment is portable and easy to operate, fluorescent probe technology can be used in high-throughput screening, which helps to facilitate widespread use in large populations. Taken together, these characteristics make fluorescent probes an ideal tool for early detection and screening of cancer.

[0007] There are fluorescent detectors on the market for early detection of cancer, which are usually based on fluorescent probes and optical biosensor technology. Fluorescent probes have the advantages of high sensitivity, non-invasiveness and real-time response in detecting cancer-related biomarkers. A variety of cancer detection devices are being developed and promoted, including early detection tools for various types of cancer, such as high-precision screening through fluorescently labeled biomarkers. Such instruments are often used to detect carcinogenic toxins in feces and cancer-related biomarkers in body fluids such as blood and saliva.

[0008] Existing fluorescence detection equipment is large-scale equipment, mainly used in laboratories. The main problems in cancer screening include high cost, complexity of test samples and challenges in standardization of results. In addition, the portability of existing fluorescent probe equipment is also limited, so it cannot be used in large-scale screening, which faces challenges. Summary of the invention

[0009] The purpose of the present invention is to provide a handheld detector based on green fluorescent probe detection and its detection method and application, which solves the problem that the existing fluorescent detection equipment is expensive and cannot be widely used in large-scale screening.

[0010] The present invention is achieved through the following technical solutions:

[0011] The present invention discloses a handheld detector based on green fluorescent probe detection, comprising a housing, and a sample fusion component, a fluorescence collection component and a main control board arranged in the housing, wherein a sample tube for placing the sample fusion component is arranged in the housing;

[0012] The sample fusion assembly includes a test tube, a swab and a hand-held cap. The top of the swab is fixed in the hand-held cap to form a sampling assembly. During the test, the swab stained with the sample to be tested is inserted into the test tube, in which the test solution is pre-set, and the test tube is placed in the sample tube.

[0013] A squeeze-off assembly is installed on the top of the sample tube, and the test tube runs through the squeeze-off assembly; when in use, the squeeze-off assembly is used to squeeze the test tube to squeeze off the swab;

[0014] The fluorescence collection component is arranged on the side of the sample slot;

[0015] The fluorescence collection component includes a green fluorescence sensor and a fluorescence exciter, and the green fluorescence sensor and the fluorescence exciter are connected to the main control board;

[0016] A battery assembly is arranged in the shell, and the battery assembly is connected to the main control board.

[0017] Furthermore, the extrusion breaking assembly includes an extrusion rod and an intermediate rod connected in one body, and the diameter of the intermediate rod is larger than that of the extrusion rod;

[0018] In a non-squeezed state, the squeezing rod is located outside the sample tube, and the middle rod is located in the inner cavity of the sample tube.

[0019] Furthermore, a limit block is installed on the inner side of the extrusion rod to limit the stroke of the extrusion rod.

[0020] Furthermore, a mounting hole is prefabricated on the shell, and the outer end of the extruded rod extends out of the mounting hole.

[0021] Furthermore, the green fluorescence sensor adopts a 518nm fluorescence sensor, and the central wavelength of the excitation light source of the fluorescence exciter is 488nm.

[0022] Further, the sample tube is made of a light-shielding material, and the test tube is a transparent tube;

[0023] The swab is made of a brittle material.

[0024] Furthermore, a laser transmission hole is prefabricated at the bottom of the sample tube, and the fluorescence collection component is arranged on one side of the laser transmission hole.

[0025] Further, the sample fusion component is a disposable consumable component;

[0026] The housing comprises a front housing and a rear housing which are detachably connected;

[0027] A side-opening upper cover is arranged on the upper part of the shell body, which is used for inserting the sample fusion assembly after opening.

[0028] The present invention also discloses a detection method of the handheld detector based on green fluorescent probe detection, which includes the following process:

[0029] During the test, the sample collection component is placed in the test tube, and the sample fusion component is placed in the sample slot;

[0030] Start the fluorescence exciter to excite the detection solution. The green fluorescence sensor obtains the fluorescence signal of the detection solution, which is used to obtain the background value of the detection solution and send it to the main control board.

[0031] Press the squeeze-off component, the test tube is squeezed and deformed, the swab is squeezed and broken, and the swab falls into the detection solution below. The sample to be tested fuses with the detection solution to react. After fluorescence excitation, the detection solution produces a change in the fluorescence signal. The green fluorescence sensor collects a new fluorescence signal again and sends it to the main control board;

[0032] The main control board obtains the difference based on the background value of the detection solution and the new fluorescence signal, outputs the concentration of the sample to be detected and displays it.

[0033] The present invention also discloses the application of the handheld detector based on green fluorescence detection in cancer screening, wherein the handheld detector is used to detect the concentration of microorganisms and microbial toxins.

[0034] Compared with the prior art, the present invention has the following beneficial technical effects:

[0035] The invention discloses a handheld detector based on green fluorescent probe detection, comprising a shell, wherein a sample fusion component and a fluorescence collection component are arranged in the shell, wherein the sample fusion component comprises a reagent tube, a swab and a handheld cap, wherein the top of the swab is fixed in the handheld cap to form a sampling component; a squeeze-off component is installed on the top of the sample tube, wherein the test tube runs through the squeeze-off component, and when in use, the squeeze-off component squeezes the test tube to squeeze off the swab, and the swab falls into the test tube and is fused with a detection solution; a fluorescence collection component is arranged on the side of each sample fusion component, wherein the fluorescence collection component comprises a green fluorescence sensor and a fluorescence exciter, and after the sampled swab falls into the reagent tube, it will undergo a fusion reaction with the detection solution to perform fluorescence excitation, and the fluorescence sensor obtains the change of the fluorescence signal, and after being processed by the main control board, the concentration of the pollutant to be detected is directly output. Using this detector, the concentration of carcinogenic toxins in the sample to be tested can be quickly detected. The fluorescent color development has high sensitivity and the detection speed is fast without waiting. It no longer needs to be sent to a professional institution for testing. The general public can also test it by themselves without the need for professional operation. It is suitable for self-testing at home. This detector is used to detect microorganisms and microbial toxins. It has a simple structure, low cost, strong portability, high stability and strong specificity.

[0036] Furthermore, the extrusion assembly includes an extrusion rod and an intermediate rod connected together. The diameter of the intermediate rod is larger than that of the extrusion rod. When the extrusion rod moves inward, it can smoothly enter the sample tube. When it returns to its position, the intermediate rod will be limited in the sample tube, which can prevent the extrusion rod from being pulled off.

[0037] Furthermore, the upper cover of the shell is equipped with a permanent magnet. When the upper cover is closed, the permanent magnet on the upper cover will approach the three-axis geomagnetic sensor. The sensor will detect the change of the surrounding magnetic field, and the signal will be transmitted to the main control board. The main control board uses this signal change to obtain the closing state of the upper cover.

[0038] Furthermore, a limit block is installed on the inner side of the extrusion rod to limit the inward movement of the extrusion rod and avoid excessive inward extrusion of the extrusion rod. BRIEF DESCRIPTION OF THE DRAWINGS

[0039] Figure 1 It is a front view of a handheld detector based on green fluorescent probe detection of the present invention;

[0040] Figure 2 For the general Figure 1 Schematic diagram of the structure left after the main control board is removed;

[0041] Figure 3 A three-dimensional diagram of the internal structure of a handheld detector based on green fluorescent probe detection according to the present invention;

[0042] Figure 4 It is a schematic diagram of the assembly structure of a sample tube and a squeeze-off component in a handheld detector based on green fluorescent probe detection of the present invention;

[0043] Figure 5 It is a schematic structural diagram of a test tube in a handheld ion detector based on green fluorescent probe detection of the present invention after being installed into a squeeze-off assembly;

[0044] Figure 6 It is a schematic diagram of the structure of the test tube after being squeezed by the squeeze-off component;

[0045] Figure 7 It is a structural schematic diagram of the extrusion breaking component;

[0046] Figure 8 This is a diagram of the layout of the fluorescence collection components.

[0047] Among them, 1. Shell; 2. Squeeze-off assembly; 3. Display screen; 4. Main control board; 5. Operation button; 6. Upper cover; 7. Sample tube; 8. Fluorescence collection assembly; 9. Battery assembly; 10. Limit block; 11. Hand-held cap; 12. Test tube; 13. Swab; 14. Three-axis geomagnetic sensor;

[0048] 71. Laser transmission hole;

[0049] 21. Extrusion rod; 22. Intermediate rod;

[0050] 81. Excitation light source; 82. Excitation filter; 83. Dichroic mirror; 84. Lens; 85. Emission filter; 86. Fluorescence collection lens; 87. Fluorescence detector. DETAILED DESCRIPTION

[0051] In order to make the purpose, technical solution and advantages of the present invention more clear, the following is further described in detail with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention, that is, the embodiments described are only part of the embodiments of the present invention, not all embodiments.

[0052] The components described and shown in the drawings and embodiments of the present invention may be arranged and designed in various configurations. Therefore, the detailed description of the embodiments of the present invention provided in the following drawings is not intended to limit the scope of the claimed invention, but merely represents a selected embodiment of the present invention. Based on the drawings and embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative work are within the scope of protection of the present invention.

[0053] It should be noted that the terms "comprises", "includes" or any other variants are intended to cover non-exclusive inclusion, so that a process, element, method, article or device that includes a series of elements includes not only those elements, but also includes other elements that are not explicitly listed, or also includes elements inherent to the process, element, method, article or device. In addition, the terms "horizontal" and "vertical" are based on the orientation and positional relationship of the devices or components shown in the drawings, and are only for the purpose of better describing the present invention, rather than requiring the devices, components or equipment shown to have this specific orientation, and therefore cannot be understood as limiting the present invention.

[0054] The features and performances of the present invention are further described in detail below in conjunction with the embodiments.

[0055] like Figures 1 to 3 As shown, the present invention discloses a detector based on a green fluorescent probe, comprising a housing 1, and a sample fusion component, a fluorescence collection component 8 and a main control board 4 arranged in the housing 1, wherein a sample tube 7 for placing the sample fusion component is arranged in the housing 1. A squeeze-off component 2 is installed on the top of the sample tube 7, and a test tube 12 runs through the squeeze-off component 2; when in use, the squeeze-off component 2 is used to squeeze the test tube 12 to squeeze off the swab 13.

[0056] like Figure 2As shown, the fluorescence collection component 8 is arranged on the side of the sample slot; a battery component 9 is arranged in the housing 1, and the battery component 9 is connected to the main control board 4.

[0057] Specifically, the fluorescence collection component 8 includes a green fluorescence sensor and a fluorescence exciter, and the green fluorescence sensor and the fluorescence exciter are connected to the main control board 4 .

[0058] like Figure 8 As shown, the fluorescence exciter includes an excitation light source 81, an excitation filter 82, a dichroic mirror 83 and a lens 84, and the green fluorescence sensor includes an emission filter 85, a fluorescence collection lens 86 and a fluorescence detector 87 which are horizontally arranged in sequence.

[0059] The excitation light source 81 is a high-efficiency LED light source, which is filtered by the excitation filter 82 to an excitation light source 81 with a half-width of 10nm and a center wavelength of 488nm. The excitation light source 81 is reflected by the dichroic mirror 83 and then emitted to the sample to be tested in the test tube 12 through the lens 84. The sample to be tested emits fluorescence with a center wavelength of 518nm under the excitation light. The emitted fluorescence passes through the dichroic mirror 83 together with the reflected light of the excitation light, and the excitation light source 81 is filtered out by the emission filter 85, leaving only pure fluorescence with a center wavelength of 518nm and a half-width of 10nm. The pure fluorescence is collected by the fluorescence detector 87 after passing through the fluorescence collection lens 86. After the fluorescence detector 87 responds to the fluorescence signal, it uses a two-stage precision operational amplifier to amplify the signal, and after amplification, it is connected to the main control board 4, and the analog quantity is collected through the AD acquisition chip.

[0060] like Figure 4 and Figure 5 As shown, the sample fusion component is a disposable consumable component, which includes a test tube 12, a swab 13 and a handheld cap 11. The top of the swab 13 is fixed in the handheld cap 11 to form a sampling component. During the detection, the swab 13 stained with the sample to be tested is inserted into the test tube 12, in which the test tube 12 is pre-filled with a detection solution. The test tube 12 is placed in the sample tube 7, and fluorescence excitation is performed to obtain a fluorescence signal.

[0061] like Figure 7 As shown, the extrusion assembly 2 includes an extrusion rod 21 and an intermediate rod 22 connected together, and the diameter of the intermediate rod 22 is larger than that of the extrusion rod 21; Figure 4 As shown, when not squeezed, the squeezing rod 21 is located outside the sample tube 7, and the middle rod 22 is located in the inner cavity of the sample tube 7. The middle rod 22 is designed to have a large diameter and the squeezing rod 21 has a small diameter. When the squeezing rod 21 moves to the right, it can smoothly enter the sample tube 7. When returning to its original position, the middle rod 22 will be limited in the sample tube 7, which can prevent the squeezing rod 21 from being pulled off.

[0062] Preferably, Figure 2As shown, a limit block 10 is also installed on the inner side of the extrusion rod 21 to limit the inward movement of the extrusion rod 21 and prevent the extrusion rod 21 from being excessively extruded inward.

[0063] like Figure 2 As shown, a mounting hole is prefabricated on the housing 1, and the outer end of the extrusion rod 21 extends out of the mounting hole, which is convenient for operators to press.

[0064] Preferably, the swab 13 is made of a brittle material, such as Figure 5 As shown, when the test tube 12 is squeezed and plastically deformed, the swab 13 can be easily broken.

[0065] Preferably, the test tube 12 is a transparent tube and will not interfere with the fluorescent signal.

[0066] like Figure 4 As shown, a laser transmission hole 71 is prefabricated at the bottom of the sample tube 7 , and the fluorescence collection component 8 is arranged on one side of the laser transmission hole 71 , so that the excitation light of the excitation light source 81 can be incident into the test tube 12 .

[0067] Specifically, the housing 1 includes a front housing and a rear housing that are detachably connected, which facilitates installation and disassembly.

[0068] A side-opening upper cover 6 is provided on the upper portion of the housing 1 for inserting the sample fusion assembly after opening.

[0069] The main control board 4 is a PCB board, on which a display screen 3 and an operation button 5 are integrated. The operation button 5 is composed of a plurality of micro switches, including power on / off, confirm, cancel, up, down, left, and right, which are connected to the main control board 4 respectively and are responsible for the operation of the whole machine and the query of data.

[0070] Display screen 3 uses a 4.3-inch IPS color screen, and the display content includes: time, storage capacity status, battery status, acquisition waveform curve, cover closing status, fusion status, and acquisition serial number.

[0071] Specifically, the acquisition sequence number is generated by combining a timestamp and a sequence number, which can be understood as an SN number.

[0072] A permanent magnet and a three-axis geomagnetic sensor 14 are mounted on the upper cover 6. The three-axis geomagnetic sensor 14 can detect the approaching state of the permanent magnet on the upper cover 6, indicating that the cover has been closed. At the same time, a sealing gasket and a magnetic attraction structure are provided on the upper cover 6. When the cover is closed, the magnetic attraction structure is used to close it tightly and prevent it from opening automatically.

[0073] Preferably, a permanent magnet is installed at the inner end of the extrusion rod 21, and a three-axis geomagnetic sensor 14 is installed on the limit block 10. During the extrusion process, the magnetic field around the three-axis geomagnetic sensor 14 will produce a magnetic change due to the approach of the permanent magnet, so that the three-axis geomagnetic sensor 14 will collect an extrusion response signal, and use this signal to obtain the extrusion state, thereby inferring that the swab 13 has fallen and starting the solution fusion process.

[0074] At the same time, the three-axis geomagnetic sensor 14 will continuously monitor the surrounding earth's magnetic field information to confirm the spatial direction of the device. When the device falls over, the three-axis geomagnetic sensor 14 will detect the change in the direction information of the earth's magnetic field, and deduce that the spatial direction of the device has changed. It can be determined that it has fallen over based on the magnetic field characteristics. At this time, the device will immediately issue an audible and visual alarm to remind the user to avoid causing additional losses.

[0075] The shell 1 component and the upper cover 6 component are all made of plastic injection molding. Because the fluorescence test is sensitive to light leakage, the shell 1, the upper cover 6, and the sample fusion component all need special designs to avoid all possible light leakage.

[0076] In the specific design, the housing 1, the upper cover 6 and the sample tube 7 are made of opaque materials, and the handheld cap 11 in the sample fusion assembly is also made of opaque materials.

[0077] The present invention also discloses a detection method of the handheld metal ion detector based on green fluorescent probe detection, which includes the following process:

[0078] During the test, the sample collection component is placed in the test tube 12, and the sample fusion component is placed in the sample slot;

[0079] The fluorescence exciter is started to excite the detection solution. The fluorescence sensor obtains the fluorescence signal of the detection sample solution, which is used to obtain the background value of the detection solution and send it to the main control board 4;

[0080] Press and squeeze component 2, such as Figure 6 As shown, the test tube 12 is squeezed and deformed, and a permanent magnet is arranged at the end of the squeeze-off assembly 2. The three-axis geomagnetic sensor detects the squeeze-off action at the same time and makes a time mark for data analysis reference. At the same time, the swab 13 is squeezed and broken, and the swab 13 falls into the detection solution below. The sample to be tested merges with the detection solution to react. After fluorescence excitation, the detection solution produces a change in the fluorescence signal. The green fluorescence sensor collects a new fluorescence signal again and sends it to the main control board 4. The main control board 4 obtains the difference based on the background value of the detection solution and the new fluorescence signal, outputs the concentration of the sample to be tested and displays it.

[0081] During the entire acquisition process, the fluorescence exciter continues to excite, and the fluorescence data will be fully recorded to form a waveform curve.

[0082] The present invention focuses on the rapid detection of microorganisms and microbial toxins by developing high-sensitivity fluorescent probes suitable for samples. For example, for common cancer-related toxins such as Bacteroides fragilis toxins, Escherichia coli genotoxins, and cyclotine peptide toxins secreted by certain Escherichia coli and Campylobacter, fluorescent probes can produce detectable fluorescent signals by specific binding to target toxins. Combined with a portable fluorescence detection instrument, this method can achieve rapid response and quantitative analysis of toxins. This innovative mode of combining probes and instruments can not only significantly improve the detection sensitivity, but also greatly simplify the operating steps, allowing non-professionals to perform microbial and microbial toxin detection in daily environments, thereby promoting the popularization and application of consumer-grade health screening tools.

[0083] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the above embodiments, ordinary technicians in the relevant field should understand that the specific implementation methods of the present invention can still be modified or replaced by equivalents. Any modification or equivalent replacement that does not depart from the spirit and scope of the present invention should be covered within the scope of protection of the claims of the present invention.

Claims

1. A handheld detector based on green fluorescent probe detection, characterized in that: It comprises a housing (1), and a sample fusion component, a fluorescence collection component (8) and a main control board (4) arranged in the housing (1); a sample tube (7) for placing the sample fusion component is arranged in the housing (1); The sample fusion component comprises a test tube (12), a swab (13) and a hand-held cap (11), wherein the top end of the swab (13) is fixed in the hand-held cap (11) to form a sampling component; during detection, the swab (13) stained with the sample to be detected is inserted into the test tube (12), the test tube (12) is pre-filled with a detection solution, and the test tube (12) is placed in the sample tube (7); A squeeze-off assembly (2) is installed on the top of the sample tube (7), and the test tube (12) passes through the squeeze-off assembly (2); when in use, the squeeze-off assembly (2) is used to squeeze the test tube (12) to squeeze off the swab (13); The fluorescence collection component (8) is arranged on the side of the sample slot; The fluorescence collection component (8) comprises a green fluorescence sensor and a fluorescence exciter, and the green fluorescence sensor and the fluorescence exciter are connected to the main control board (4); A battery assembly (9) is arranged in the housing (1), and the battery assembly (9) is connected to the main control board (4).

2. A handheld detector based on green fluorescent probe detection according to claim 1, characterized in that: The extrusion assembly (2) comprises an extrusion rod (21) and an intermediate rod (22) which are connected together, and the diameter of the intermediate rod (22) is larger than that of the extrusion rod (21); In the non-squeezed state, the squeezing rod (21) is located outside the sample tube (7), and the intermediate rod (22) is located in the inner cavity of the sample tube (7).

3. A handheld detector based on green fluorescent probe detection according to claim 2, characterized in that: A limit block (10) is also installed on the inner side of the extrusion rod (21) to limit the travel of the extrusion rod (21).

4. A handheld detector based on green fluorescent probe detection according to claim 1, characterized in that: A mounting hole is prefabricated on the housing (1), and the outer end of the extrusion rod (21) extends out of the mounting hole.

5. A handheld detector based on green fluorescent probe detection according to claim 1, characterized in that: The green fluorescence sensor adopts a 518nm fluorescence sensor, and the central wavelength of the excitation light source (81) of the fluorescence exciter is 488nm.

6. A handheld detector based on green fluorescent probe detection according to claim 1, characterized in that: The sample tube (7) is made of a light-shielding material, and the test tube (12) is a transparent tube; The swab (13) is made of a brittle material.

7. A handheld detector based on green fluorescent probe detection according to claim 1, characterized in that: A laser transmission hole (71) is prefabricated at the bottom of the sample tube (7), and the fluorescence collection component (8) is arranged on one side of the laser transmission hole (71).

8. A handheld detector based on green fluorescent probe detection according to claim 1, characterized in that: The sample fusion component is a disposable consumable component; The housing (1) comprises a front housing and a rear housing which are detachably connected; A side-opening upper cover (6) is provided on the upper part of the housing (1) for inserting the sample fusion assembly after opening.

9. The present invention also discloses a detection method of a handheld detector based on green fluorescent probe detection as described in any one of claims 1 to 8, characterized in that: The process includes: During the test, the sample collection component is placed in a test tube (12), and the sample fusion component is placed in a sample slot; The fluorescence exciter is started to excite the detection solution, and the green fluorescence sensor obtains the fluorescence signal of the detection solution, which is used to obtain the background value of the detection solution and send it to the main control board (4); The squeeze-off component (2) is pressed, the test tube (12) is squeezed and deformed, the swab (13) is squeezed and broken, and the swab (13) falls into the detection solution below, the sample to be tested fuses with the detection solution to react, and after fluorescence excitation, the detection solution produces a change in the fluorescence signal, and the green fluorescence sensor collects a new fluorescence signal again and sends it to the main control board (4); The main control board (4) obtains a difference based on the background value of the detection solution and the new fluorescence signal, outputs the concentration of the sample to be detected and displays it.

10. Use of the handheld detector based on green fluorescent probe detection according to any one of claims 1 to 8 in cancer screening, characterized in that: The handheld detector is used to detect the concentration of microorganisms and microbial toxins.

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