Nutrient composition beneficial to height growth and application thereof

Abstract

The invention provides a nutritional composition for promoting secretion of growth hormone so as to promote height growth and application thereof, the nutritional composition comprises lysine, seaweed calcium and bovine colostrum basic protein, and the composition has synergistic effects in promoting secretion of growth hormone, skeletal development and the like. Through in-vitro tests, the composition can significantly improve the levels of growth regulatory factor IGF-1 and growth hormone HGH, significantly enhance bone density, improve osteocalcin level, reduce urine DPD level, promote bone formation and absorption, and significantly promote height growth.

Description

Technical Field

[0001] The invention belongs to the field of medical technology and nutritional supplements, and in particular relates to a composition that helps height growth and an application thereof. Background Art

[0002] Since children need a large amount of nutrition during their growth and development, and their ability to absorb nutrition is also somewhat insufficient, during the development of children, the reasonable consumption of nutritional supplements can not only ensure the nutritional balance of children, but also promote their healthy growth. However, if children consume excessive nutritional supplements, it is likely to lead to various nutritional imbalances, thus affecting their growth and development. Therefore, in order to ensure that children can grow up healthily and healthily, increasing research on the effects of nutritional supplements on children's growth and development is not only of great significance, but also urgent.

[0003] The so-called nutritional supplements are foods containing specific nutrients that are produced to make up for the nutrients that may be insufficient in the normal human diet and are necessary for the human body. Common nutritional supplements generally contain vitamins, minerals, amino acids and other ingredients.

[0004] Vitamins play a vital role in children's growth and development. They are not only necessary components for maintaining normal physiological functions, but some specific types of vitamins have also been shown to have significant effects in promoting height growth. For example, vitamin A also plays a key role in regulating bone growth and cell differentiation. A proper level of vitamin A is essential for maintaining normal growth hormone secretion and bone development. Adequate intake of B vitamins, especially vitamin B2 (riboflavin), also contributes to the normal growth and development of children. Riboflavin plays a basic role in energy metabolism and cellular respiration and is an essential component for physical development and biochemical reactions. Mineral supplementation can significantly promote children's height growth. Minerals such as calcium, magnesium, zinc and iron play an irreplaceable role in participating in bone formation, enhancing bone density, promoting cell proliferation and protein synthesis. In the process of children's growth and development, in addition to vitamins and minerals, certain amino acids also play a key role. Lysine is an essential amino acid that cannot be synthesized by the human body and must be obtained through diet or supplements. Lysine plays a key role in promoting protein synthesis, calcium absorption and growth hormone secretion.

[0005] Patent document CN118592621A discloses a nutritional supplement for the height growth and development of children and adolescents, including multiple ingredients such as minerals and trace elements, amino acids and their derivatives. It mainly focuses on the digestion and absorption of the active ingredients in the nutritional supplement in the human body, and provides comprehensive nutritional support, but lacks research on the effects of the active ingredients in the nutritional supplement on growth hormone levels, bone development, etc.

[0006] Although most of the nutritional supplements provided by the prior art contain a variety of nutrients, they still generally have the problem of incomplete nutrition and ineffective effects. Therefore, it is necessary to provide a nutritional supplement with certain effective ingredients for humans, especially children and / or adolescents, to promote human growth and development. Summary of the invention

[0007] In view of the problems existing in the prior art, the present invention provides a nutritional composition that helps height growth and its application. The nutritional composition includes lysine, seaweed calcium and bovine colostrum basic protein. The composition has a synergistic effect in improving the level of growth hormone IGF-1, the level of HGH, and promoting bone development.

[0008] In one aspect, the present invention provides a nutritional composition for promoting growth hormone secretion and thus promoting height growth, wherein the nutritional composition comprises lysine, seaweed calcium and bovine colostrum basic protein, and the growth hormone comprises IGF-1 and HGH.

[0009] Lysine is an essential amino acid that plays a key role in promoting protein synthesis, calcium absorption and growth hormone secretion. On the one hand, lysine is the basis of protein synthesis, and a lack of lysine can lead to growth stagnation in children; on the other hand, lysine can improve the intestinal absorption of calcium; on the other hand, lysine can also stimulate the secretion of growth hormone and promote bone growth. At the same time, supplementing enough minerals is of great significance to ensure the normal growth and development of children.

[0010] In some embodiments, the lysine is L-lysine, with a chemical formula of C6H 14 N2O2, CAS No: 56-87-1. Lysine is preferably provided in the form of a pharmaceutically acceptable salt, in particular in the form of (L)-lysine-hydrochloride.

[0011] Calcium is the main component of bones, and about 99% of calcium is stored in bones and teeth. High calcium intake in childhood can effectively increase bone density and promote height growth. Seaweed calcium, as a natural mineral, contains pure natural ion ingredients such as spirulina and biological calcium carbonate, as well as vitamin D and natural enzymes. Vitamins and natural enzymes can increase the absorption rate of calcium. Seaweed calcium can not only replenish the calcium needed in the body, but also contains iron, zinc, manganese, iodine, potassium, magnesium and other nutrients. After being absorbed by human tissues, they can usually be quickly replenished, promote their own growth and development, and make bones stronger.

[0012] Bovine colostrum alkaline protein is an alkaline protein extracted from bovine colostrum, which has unique physiological activity. Bovine colostrum alkaline protein is rich in calcium, which can promote the growth and development of bones and has a significant effect on the height growth of children. However, there are no reports on the effects of vitamin D and bovine colostrum alkalinity on growth hormone.

[0013] IGF-1 (insulin-like growth factors-1) is a polypeptide protein substance similar to insulin in molecular structure. Its full name is "insulin-like growth factor", also known as somatomedin. IGF-1 can promote the production of growth hormone and regulate the growth, development and metabolism of bones. At the same time, growth hormone (Human Growth Hormone, HGH) is a peptide hormone secreted by the anterior pituitary gland of the human body. It is composed of 191 amino acids and can promote the growth of bones, internal organs and the whole body. It promotes protein synthesis, affects fat and mineral metabolism, and also plays a key role in human growth and development.

[0014] Therefore, the present invention explores the effects of lysine, seaweed calcium, bovine colostrum alkaline protein and their combination on growth hormone. Comparative experiments have shown that after the three components of lysine, seaweed calcium and bovine colostrum alkaline protein are combined, they have obvious synergistic effects in improving growth hormone levels and promoting bone development. Further screening experiments have shown that the nutritional composition prepared by replacing seaweed calcium with calcium lactate or calcium carbonate, or the nutritional composition prepared by replacing bovine colostrum alkaline protein with whey protein or collagen, is not as effective as the combination of lysine, seaweed calcium and bovine colostrum alkaline protein in promoting growth hormone secretion, so the present invention preferably uses lysine, seaweed calcium and bovine colostrum alkaline protein. Nutritional composition.

[0015] Furthermore, the nutritional composition also includes glycine and glutamine.

[0016] Glycine is a non-essential amino acid, but it plays an important role in children's bone development. Glycine is the main component of collagen, which is essential in the formation of bones and connective tissue. Studies have found that glycine deficiency affects collagen synthesis, thereby affecting bone development, while adequate glycine intake contributes to healthy bone growth and repair.

[0017] In some embodiments, the chemical formula of glycine is C2H5NO2, CAS No: 56-40-6.

[0018] Glutamine is the most abundant free amino acid in the body and has multiple physiological functions, including promoting protein synthesis, enhancing immune function, and maintaining intestinal health. First, glutamine plays an important role in protein synthesis and nitrogen balance. Supplementing glutamine helps promote muscle and bone growth in children. Second, glutamine can enhance the function of the immune system and help reduce the negative impact of illness on growth. Third, glutamine helps improve nutrient absorption, thereby supporting children's growth and development.

[0019] In some embodiments, the glutamine chemical formula is C5H 10 N2O3, CAS No: 56-85-9.

[0020] In some embodiments, glycine and glutamine are preferably provided in monomeric and free form, particularly in the form of glycine and L-glutamine.

[0021] Screening and comparative experiments have shown that nutritional compositions with different amino acid components have different promoting effects on the secretion of growth hormone. The nutritional composition including arginine and / or histidine components is not as effective in promoting the secretion of growth hormone as the nutritional composition including glycine and glutamine. The preferred nutritional composition is lysine, seaweed calcium, bovine colostrum basic protein, glycine, glutamine, vitamin D and vitamin K2.

[0022] Furthermore, the nutritional composition also includes vitamin D (calciferol) and vitamin K2 (menaquinone-7).

[0023] Vitamin D is one of the main vitamins that promotes bone health and growth. Vitamin D helps form and maintain strong bones by promoting the absorption of calcium and phosphorus. Vitamin K2 also plays an important role in children's bone health and growth. Vitamin K2 promotes the utilization of calcium by activating osteocalcin, so that it is effectively deposited in the bones instead of accumulating in the blood vessels. The appropriate amount of vitamin K2 intake can significantly increase bone density and bone strength, thereby promoting the growth of children's height. Supplementing with sufficient vitamins, especially vitamin D, vitamin K2, etc., has a significant effect on promoting children's height growth.

[0024] Studies have shown that adding glycine, glutamine, vitamin D and vitamin K2 to lysine, seaweed calcium and bovine colostrum basic protein can further increase IGF-1 and HGH levels, further enhance bone density, and improve indicators such as osteocalcin, thereby promoting bone development.

[0025] Furthermore, the mass ratio of lysine, seaweed calcium, bovine colostrum basic protein, glycine, glutamine, vitamin D and vitamin K2 is (300-400): (50-100): (1-10): (10-20): (10-20): (0.001-0.004): (1-4).

[0026] In some embodiments, the mass ratio of lysine, seaweed calcium, and bovine colostrum basic protein is (300-350): (50-80): (5-10).

[0027] In another aspect, the present invention provides a product for promoting the secretion of growth hormone and thus promoting height growth, comprising the nutritional composition as described above.

[0028] In some embodiments, the nutritional composition further comprises minerals such as magnesium oxide, ferrous fumarate, zinc gluconate, etc. Minerals such as calcium, magnesium, zinc and iron play an irreplaceable role in participating in bone formation, enhancing bone density, promoting cell proliferation and protein synthesis, etc.

[0029] In some embodiments, the nutritional composition further comprises vitamin A (retinyl acetate), vitamin B1 (thiamine nitrate), vitamin B2 (riboflavin), vitamin B5 (calcium pantothenate), vitamin B6 (pyridoxine hydrochloride), vitamin B9 (folic acid), vitamin B12 (cyanocobalamin), vitamin H (biotin), vitamin K3 (menadione), and vitamin E (tocopherol acetate).

[0030] In some embodiments, the composition further comprises bromelain and papain to promote digestion and absorption.

[0031] The nutritional composition provided by the present invention can add suitable ingredients such as diluents, excipients, adhesives, bulking agents, preservatives, colorants, thickeners, anticaking agents, sweeteners, stabilizers and other drugs or food processing agents as needed within the scope that does not impair the effects of the present invention.

[0032] In some embodiments, the thickener includes starch, maltodextrin, sodium carboxymethyl cellulose, etc., the anti-caking agent includes magnesium stearate, anhydrous silicon dioxide, anhydrous calcium hydrogen phosphate dihydrate, etc.; the sweetener includes glucose, sucralose, erythritol, etc.; the stabilizer includes microcrystalline cellulose, etc.

[0033] In some embodiments, the composition can be prepared into a variety of administration forms, and the dosage forms for oral administration include chewable tablets, elixirs, liquids, solutions, suspensions, emulsions, capsules, soft capsules, hard capsules, tablets, powders, soft candies, orally dissolving tablets, capsules, lozenges, candies and combinations thereof. The capsule can be a hard capsule, such as a two-piece capsule, or a soft capsule. In embodiments provided by the present invention, the nutritional composition includes a tablet form.

[0034] In another aspect, the present invention provides a use of a composition for preparing an agent for increasing the level of growth factor IGF-1, wherein the composition comprises lysine, seaweed calcium, and bovine colostrum basic protein.

[0035] Furthermore, the composition also includes glycine, glutamine, vitamin D and vitamin K2.

[0036] Furthermore, the mass ratio of lysine, seaweed calcium, bovine colostrum basic protein, glycine, glutamine, vitamin D and vitamin K2 is (300-400): (50-100): (1-10): (10-20): (10-20): (0.001-0.004): (1-4).

[0037] In some embodiments, the mass ratio of lysine, seaweed calcium, and bovine colostrum basic protein is (300-350): (50-80): (5-10).

[0038] In another aspect, the present invention provides a use of a composition for preparing an agent for increasing the level of growth hormone HGH, wherein the composition comprises lysine, seaweed calcium, and bovine colostrum basic protein.

[0039] Furthermore, the composition also includes glycine, glutamine, vitamin D and vitamin K2.

[0040] Furthermore, the mass ratio of lysine, seaweed calcium, bovine colostrum basic protein, glycine, glutamine, vitamin D and vitamin K2 is (300-400): (50-100): (1-10): (10-20): (10-20): (0.001-0.004): (1-4).

[0041] In some embodiments, the mass ratio of lysine, seaweed calcium, and bovine colostrum basic protein is (300-350): (50-80): (5-10).

[0042] In another aspect, the present invention provides a use of a composition for preparing an agent for increasing osteocalcin levels, wherein the composition comprises lysine, algae calcium, and bovine colostrum basic protein.

[0043] In another aspect, the present invention provides a use of a composition for preparing an agent for enhancing bone density, wherein the composition comprises lysine, seaweed calcium, and bovine colostrum basic protein.

[0044] In another aspect, the present invention provides a use of a composition for preparing an agent for reducing urinary DPD levels, wherein the composition comprises lysine, seaweed calcium, and bovine colostrum basic protein.

[0045] The present invention proves through comparative studies that the combination of lysine, seaweed calcium and bovine colostrum basic protein has obvious synergistic effects in improving osteocalcin levels, enhancing bone density, and reducing urine DPD levels; on the basis of lysine, seaweed calcium and bovine colostrum basic protein, adding glycine, glutamine, vitamin D and vitamin K2 can further improve osteocalcin levels, enhance bone density, and reduce urine DPD concentration, thereby promoting bone formation and absorption, and achieving the effect of promoting height growth.

[0046] Furthermore, the mass ratio of lysine, seaweed calcium, bovine colostrum basic protein, glycine, glutamine, vitamin D and vitamin K2 is (300-400): (50-100): (1-10): (10-20): (10-20): (0.001-0.004): (1-4).

[0047] In some embodiments, the mass ratio of lysine, seaweed calcium, and bovine colostrum basic protein is (300-350): (50-80): (5-10).

[0048] Compared with the prior art, the present invention has the following beneficial effects:

[0049] 1. Provided is a composition that helps promote height growth and has a synergistic effect in increasing the levels of somatomedin IGF-1 and growth hormone HGH;

[0050] 2. The composition provided by the present invention further enhances bone density, increases osteocalcin levels, and reduces urine DPD levels by promoting growth hormone secretion, thereby promoting bone formation and development;

[0051] 3. The raw materials of the formula group are easily available, safe and healthy, and suitable for large-scale production and application. DETAILED DESCRIPTION

[0052] The present invention is further described in detail below in conjunction with the examples. It should be noted that the examples described below are intended to facilitate the understanding of the present invention and do not have any limiting effect on it. The reagents used in this example are all known products and were obtained by purchasing commercially available products.

[0053] Example 1: Preparation of the three-component nutritional composition provided by the present invention

[0054] Lysine (purchased from Evonik Industries AG), seaweed calcium (purchased from Aquamin), and bovine colostrum basic protein (purchased from Fonterra) were weighed in a mass ratio of (300-400): (50-100): (1-10), preferably 305:52:8 in this embodiment, and starch was used as a filler to prepare a composition. The contents of lysine, seaweed calcium, and bovine colostrum basic protein in the composition were 78.45%, 13.38%, and 2.06%, respectively.

[0055] Example 2, Lysine + Seaweed Calcium

[0056] Lysine (purchased from Evonik Industries AG) and seaweed calcium (purchased from Aquamin) were weighed in a mass ratio of (300-400): (50-100), preferably 305:52 in this embodiment, and starch was used as a filler to prepare a composition. The contents of lysine and seaweed calcium in the composition were 78.45% and 13.38%, respectively.

[0057] Example 3, Lysine + bovine colostrum basic protein

[0058] Lysine (purchased from Evonik Industries AG) and bovine colostrum basic protein (purchased from Fonterra) were weighed in a mass ratio of (300-400): (1-10), preferably 305:8 in this embodiment, and starch was used as a filler to prepare a composition. The contents of lysine and bovine colostrum basic protein in the composition were 78.45% and 2.06%, respectively.

[0059] Example 4: Seaweed calcium + bovine colostrum basic protein

[0060] Seaweed calcium (purchased from Aquamin) and bovine colostrum alkaline protein (purchased from Fonterra) were weighed in a mass ratio of (50-100): (1-10), preferably 52:8 in this embodiment, and starch was used as a filler to prepare a composition. The contents of seaweed calcium and bovine colostrum alkaline protein in the composition were 13.38% and 2.06%, respectively.

[0061] Example 5: Effect of the three-component nutritional composition on IGF-1 and HGH levels

[0062] The nutritional compositions prepared in Examples 1 to 4 and Samples 1 to 3 shown in Table 1 were used for growth hormone IGF-1 level test, respectively, wherein the contents of lysine, seaweed calcium, and bovine colostrum basic protein in Samples 1 to 3 were 78.45%, 13.38%, and 2.06%, respectively. The specific method of the experiment is: experimental rats are used as test objects, 15 rats in each group, and 21 experimental groups are set up. The rats in the experimental groups are given the nutritional compositions prepared in Samples 1 to 3 and Examples 1 to 4, respectively, and 100 mg of the samples are given per kg of rat body weight for treatment. The blank control group is given physiological saline, and the negative control is given starch. The treatment method is to take the sample orally once a day, and the experimental period is 30 days. At the end of the experimental period, blood samples of the experimental rats are collected. Afterwards, the concentration of growth hormone IGF-1 is detected using an ELISA kit, and the experimental results are shown in Table 1; the concentration of growth hormone HGH is detected using a radioimmunoassay (RIA) method, and the experimental results are shown in Table 2.

[0063] Table 1. Results of IGF-1 test

[0064]

[0065] Table 2 Growth hormone HGH test results

[0066]

[0067] According to the data in Table 1 and Table 2, by comparing samples 1 to 3 with the control, it can be seen that the lysine component alone can promote the secretion of somatomedin IGF-1 and increase the level of growth hormone HGH, and seaweed calcium and bovine colostrum basic protein have no effect on the levels of somatomedin IGF-1 and growth hormone HGH; by comparing the control with Examples 1 to 4, it can be seen that compared with the two active ingredients, the combination of the three active ingredients of lysine, seaweed calcium and bovine colostrum basic protein (Example 1) has a significant synergistic effect in promoting the secretion of somatomedin IGF-1, and the combination of the three active ingredients of lysine, seaweed calcium and bovine colostrum basic protein is preferred.

[0068] Example 6: Effect of the three-component nutritional composition on bone development

[0069] Serum calcium concentration, bone density and osteocalcin are key indicators for measuring bone health and development. In general, blood calcium concentration should be maintained within a normal range to ensure that the bone mineralization process proceeds normally. If the serum calcium concentration is too low, it may indicate insufficient calcium intake or insufficient calcium storage in the bones, affecting bone development. Bone density is a key indicator of bone strength. Higher bone density is usually associated with stronger bone structure, while lower bone density may indicate osteoporosis or skeletal dysplasia. Osteocalcin (OC) levels can reflect the activity of osteoblasts. If the osteocalcin level is high, it usually indicates increased bone formation activity, which may be a manifestation of bone growth or repair. At the same time, fluctuations in the level of deoxypyridinoline (DPD) in urine can be used to assess the balance of bone metabolism. If the DPD level in urine is elevated, it usually indicates increased bone resorption (bone loss) activity, which may indicate osteopenia and skeletal dysplasia; on the contrary, a decrease in the DPD level in urine indicates good bone resorption.

[0070] In order to explore the effect of the three-component nutritional composition on bone development, the nutritional composition prepared in Examples 1 to 4 and Samples 1 to 3 shown in Table 3 were used for key indicator level tests of bone development and health, respectively. The contents of lysine, seaweed calcium, and bovine colostrum basic protein in Samples 1 to 3 were 78.45%, 13.38%, and 2.06%, respectively. The specific treatment method was the same as that in Example 5, using atomic absorption spectrometry (AAS) to detect serum calcium concentration; using dual-energy X-ray absorptiometry (DEXA) to detect bone density; and using enzyme-linked immunosorbent assay (ELISA) to measure DPD and osteocalcin levels in urine. The test results are shown in Table 3.

[0071] Table 3 Results of bone density and bone growth marker level tests

[0072]

[0073] By comparing samples 1 to 3 with the control, it can be seen that the individual seaweed calcium and bovine colostrum basic protein components can increase the level of osteocalcin, enhance bone density, and reduce the level of urinary DPD, and there is no significant change in the calcium concentration in serum. The individual lysine component has little effect on these indicators. By comparing the control with Examples 1 to 4, it can be seen that the composition with only two components has no obvious effect on bone development indicators. The combination of the three effective ingredients of lysine, seaweed calcium, and bovine colostrum basic protein (Example 1) has a significant synergistic effect in increasing the level of osteocalcin, enhancing bone density, and reducing the level of urinary DPD. The combination of the three components of lysine, seaweed calcium, and bovine colostrum basic protein is preferred.

[0074] Example 7: Effects of seaweed calcium and bovine colostrum basic protein on IGF-1 levels

[0075] Seaweed calcium is a plant calcium, rich in calcium, and is also easily absorbed by the human body; at the same time, bovine colostrum basic protein can directly act on bone cells to promote bone metabolism; and coordinate the activities of osteoblasts and osteoclasts, maintain a dynamic balance between the two, promote bone growth, and repair bone. These two substances have a good promoting effect on bone development. In order to further explore whether these two ingredients have an effect on the secretion of growth hormone IGF-1 and verify the difference between them and other calcium supplement ingredients, this example provides different nutritional composition ingredient combinations shown in Table 4. The experimental method and growth regulator IGF-1 concentration detection are the same as in Example 5, and the test results are shown in Table 4.

[0076] Table 4 Screening of different nutritional composition ingredients

[0077]

[0078] According to the data in Table 4, by comparing combinations 1 to 5 with the control, it was found that different calcium substances and proteins combined with lysine can improve the level of growth hormone, but by comparing combinations 1 to 3, it can be seen that after replacing seaweed calcium with calcium lactate or calcium carbonate, the promoting effect on IGF-1 concentration is not obvious, which is not as good as the combination of lysine, seaweed calcium and bovine colostrum basic protein (combination 1); by comparing combinations 1, 4, and 5, it can be seen that after replacing bovine colostrum basic protein with whey protein or collagen, the two compositions (combinations 4 and 5) are not as good as combination 1 in improving IGF-1 levels. Therefore, the combination of the three effective ingredients of lysine, seaweed calcium and bovine colostrum basic protein is preferred.

[0079] Example 8: Preparation of the seven-component nutritional composition provided by the present invention

[0080] Lysine (purchased from Evonik Industries AG), seaweed calcium (purchased from Aquamin), bovine colostrum basic protein (purchased from Fonterra), glycine (purchased from Evonik Industries AG), glutamine (purchased from Evonik Industries AG), vitamin D (purchased from DSM) and vitamin K2 (purchased from DSM) are weighed according to a mass ratio of (300-400): (50-100): (1-10): (10-20): (10-20): (0.001-0.004): (1-4). In this embodiment, 305:52:8:10:10:0.003:3.7575 is preferred, and starch is used as a filler to prepare a composition. The contents of lysine, seaweed calcium, bovine colostrum basic protein, glycine, glutamine, vitamin D and vitamin K2 in the composition are 78.45%, 13.38%, 2.06%, 2.57%, 2.57%, 0.07‰ and 0.97%, respectively.

[0081] Example 9, without lysine

[0082] Seaweed calcium (purchased from Aquamin), bovine colostrum basic protein (purchased from Fonterra), glycine (purchased from Evonik Industries AG), glutamine (purchased from Evonik Industries AG), vitamin D (purchased from DSM) and vitamin K2 (purchased from DSM) are weighed according to a mass ratio of (50-100): (1-10): (10-20): (10-20): (0.001-0.004): (1-4). In this embodiment, 52:8:10:10:0.003:3.7575 is preferred, and starch is used as a filler to prepare a composition. The contents of seaweed calcium, bovine colostrum basic protein, glycine, glutamine, vitamin D and vitamin K2 in the composition are 13.38%, 2.06%, 2.57%, 2.57%, 0.07‰ and 0.97%, respectively.

[0083] Example 10: No seaweed calcium

[0084] Lysine (purchased from Evonik Industries AG), colostrum basic protein (purchased from Fonterra), glycine (purchased from Evonik Industries AG), glutamine (purchased from Evonik Industries AG), vitamin D (purchased from DSM) and vitamin K2 (purchased from DSM) are weighed according to a mass ratio of (300-400): (1-10): (10-20): (10-20): (0.001-0.004): (1-4). In this embodiment, 305:8:10:10:0.003:3.7575 is preferred, and starch is used as a filler to prepare a composition. The contents of lysine, colostrum basic protein, glycine, glutamine, vitamin D and vitamin K2 in the composition are 78.45%, 2.06%, 2.57%, 2.57%, 0.07‰ and 0.97%, respectively.

[0085] Example 11: No bovine colostrum basic protein

[0086] Lysine (purchased from Evonik Industries AG), seaweed calcium (purchased from Aquamin), glycine (purchased from Evonik Industries AG), glutamine (purchased from Evonik Industries AG), vitamin D (purchased from DSM) and vitamin K2 (purchased from DSM) are weighed according to a mass ratio of (300-400): (50-100): (10-20): (10-20): (0.001-0.004): (1-4). In this embodiment, 305:52:10:10:0.003:3.7575 is preferred, and starch is used as a filler to prepare a composition. The contents of lysine, seaweed calcium, glycine, glutamine, vitamin D and vitamin K2 in the composition are 78.45%, 13.38%, 2.57%, 2.57%, 0.07‰ and 0.97%, respectively.

[0087] Example 12: No Glycine

[0088] Lysine (purchased from Evonik Industries AG), seaweed calcium (purchased from Aquamin), bovine colostrum basic protein (purchased from Fonterra), glutamine (purchased from Evonik Industries AG), vitamin D (purchased from DSM) and vitamin K2 (purchased from DSM) are weighed according to a mass ratio of (300-400): (50-100): (1-10): (10-20): (0.001-0.004): (1-4). In this embodiment, 305:52:8:10:0.003:3.7575 is preferred, and starch is used as a filler to prepare a composition. The contents of lysine, seaweed calcium, bovine colostrum basic protein, glutamine, vitamin D and vitamin K2 in the composition are 78.45%, 13.38%, 2.06%, 2.57%, 0.07‰ and 0.97%, respectively.

[0089] Example 13: No glutamine

[0090] Lysine (purchased from Evonik Industries AG), seaweed calcium (purchased from Aquamin), bovine colostrum basic protein (purchased from Fonterra), glycine (purchased from Evonik Industries AG), vitamin D (purchased from DSM) and vitamin K2 (purchased from DSM) were weighed according to a mass ratio of (300-400): (50-100): (1-10): (10-20): (0.001-0.004): (1-4). In this embodiment, 305:52:8:10:0.003:3.7575 is preferred, and starch is used as a filler to prepare a composition. The contents of lysine, seaweed calcium, bovine colostrum basic protein, glycine, vitamin D and vitamin K2 in the composition are 78.45%, 13.38%, 2.06%, 2.57%, 0.07‰ and 0.97%, respectively.

[0091] Example 14, without vitamin D

[0092] Lysine (purchased from Evonik Industries AG), seaweed calcium (purchased from Aquamin), bovine colostrum basic protein (purchased from Fonterra), glycine (purchased from Evonik Industries AG), glutamine (purchased from Evonik Industries AG) and vitamin K2 (purchased from DSM) are weighed according to a mass ratio of (300-400): (50-100): (1-10): (10-20): (10-20): (1-4). In this embodiment, 305:52:8:10:10:3.7575 is preferred, and starch is used as a filler to prepare a composition. The contents of lysine, seaweed calcium, bovine colostrum basic protein, glycine, glutamine and vitamin K2 in the composition are 78.45%, 13.38%, 2.06%, 2.57%, 2.57% and 0.97%, respectively.

[0093] Example 15: No Vitamin K2

[0094] Lysine (purchased from Evonik Industries AG), seaweed calcium (purchased from Aquamin), bovine colostrum basic protein (purchased from Fonterra), glycine (purchased from Evonik Industries AG), glutamine (purchased from Evonik Industries AG) and vitamin D (purchased from DSM) are weighed according to a mass ratio of (300-400): (50-100): (1-10): (10-20): (10-20): (0.001-0.004). In this embodiment, 305:52:8:10:10:0.003 is preferred, and starch is used as a filler to prepare a composition. The contents of lysine, seaweed calcium, bovine colostrum basic protein, glycine, glutamine and vitamin D in the composition are 78.45%, 13.38%, 2.06%, 2.57%, 2.57% and 0.07‰, respectively.

[0095] Example 16, IGF-1 and HGH level test analysis

[0096] In order to explore the promotion efficiency of different combinations of nutritional compositions on growth hormone secretion, the nutritional compositions prepared in Examples 8 to 15 were used for IGF-1 and HGH level tests, respectively. The experimental subjects and methods and the IGF-1 and HGH concentration detection methods were the same as in Example 5. The experimental results are shown in Table 5.

[0097] Table 5 Effects of different nutritional compositions on IGF-1 and HGH levels

[0098]

[0099] According to the data in Table 5, by comparing Example 1 with Examples 8 to 11, it can be seen that on the basis of the three effective ingredients of lysine, seaweed calcium and bovine colostrum basic protein, the addition of four ingredients of glycine, glutamine, vitamin D and vitamin K2 can further promote the secretion of IGF-1 and increase the concentration of HGH. However, if one of the three ingredients of lysine, seaweed calcium and bovine colostrum basic protein is missing in the composition, the promoting effect on the two growth hormones is not obvious, indicating that the further promoting effect of adding the four ingredients of glycine, glutamine, vitamin D and vitamin K2 to the nutritional composition needs to be effective when the three ingredients of lysine, seaweed calcium and bovine colostrum basic protein are present at the same time.

[0100] In addition, by comparing Examples 1, 8, 12 to 15, adding glycine, glutamine, vitamin D or vitamin K2 to the three effective ingredients of lysine, seaweed calcium and bovine colostrum basic protein can further improve the levels of IGF-1 and HGH, especially when glycine, glutamine, vitamin D and vitamin K2 are added at the same time, the concentrations of IGF-1 and HGH reach the highest, with obvious synergistic effects, which are significantly better than the effects of other combinations. Therefore, in terms of promoting growth hormone secretion, the most preferred composition is a combination of lysine, seaweed calcium, bovine colostrum basic protein, glycine, glutamine, vitamin D and vitamin K2.

[0101] Example 17: Test and analysis of key indicators of bone development and health

[0102] In order to further explore the effects of different combinations of nutritional compositions on bone development, the nutritional compositions prepared in Examples 8 to 15 were used in this example to test the key index levels of bone development and health. The experimental subjects and methods were the same as in Example 5, and the relevant index determination methods were the same as in Example 6. The test results are shown in Table 6.

[0103] Table 6 Effects of different nutritional compositions on bone development

[0104]

[0105] According to the data in Table 6, when comparing Examples 1, 8, 12 to 15, glycine, glutamine, vitamin D or vitamin K2 are added on the basis of the three effective ingredients of lysine, seaweed calcium and bovine colostrum basic protein, bone density can be further enhanced, osteocalcin levels can be increased, and urine DPD levels can be reduced. In particular, when glycine, glutamine, vitamin D and vitamin K2 are added at the same time, bone density and osteocalcin levels reach the highest, and urine DPD levels are reduced to the lowest, with obvious synergistic effects, which are better than the effects of other combinations, but have no obvious effect on serum calcium concentration. While comparing Examples 8 to 11, on the basis of the combination of lysine, seaweed calcium, bovine colostrum basic protein, glycine, glutamine, vitamin D and vitamin K2, reducing lysine or seaweed calcium or bovine colostrum basic protein has no obvious promoting effect on various indicators. Therefore, in terms of promoting bone growth and development, the most preferred composition is a combination of lysine, seaweed calcium, bovine colostrum basic protein, glycine, glutamine, vitamin D and vitamin K2.

[0106] Example 18: Screening of amino acid components

[0107] Since growth hormone is a key hormone that promotes physical growth and development, and amino acids in nutrients have a promoting effect on the secretion of growth hormone. In order to further explore the promoting effect of nutritional compositions with different amino acid components on the secretion of growth hormone, this example provides nutritional compositions with different amino acid components as shown in Table 8. The experimental method and the concentration detection of growth regulator IGF-1 are the same as those in Example 5, and the detection results are shown in Table 7.

[0108] Table 7 Screening of nutritional compositions with different amino acid components

[0109]

[0110] From the data in Table 7, by comparing combinations 1 to 4 with the control, it can be seen that the nutritional compositions with different amino acid combinations can improve the level of growth hormone, but by comparing combinations 1 to 4, it can be seen that after using arginine or histidine instead of glycine or glutamine, the nutritional composition has a less promoting effect on IGF-1 secretion than the combination of lysine, seaweed calcium, bovine colostrum basic protein, glycine, glutamine, vitamin D and vitamin K2 (combination 1). Therefore, the nutritional composition of seven ingredients, namely, lysine, seaweed calcium, bovine colostrum basic protein, glycine, glutamine, vitamin D and vitamin K2, is preferred.

[0111] Example 19: Preparation of a nutritional supplement containing a nutritional composition

[0112] (I) Preparation of Tablet Nutritional Supplements from the Nutritional Composition Provided in Example 1①

[0113] After mixing the ingredients with clear addition amounts in Table 8 and tableting excipients evenly, use 95% alcohol for granulation. Dry at 60℃ until the moisture content is below 5%, add the prepared granules, mix in an appropriate amount of microcrystalline cellulose and mix for 30 min. Add magnesium stearate and mix for 10 min, then discharge. Tablet, control the tablet weight (550 mg±27.5), ensure that the tablet surface is smooth and non-sticky, and the tablet is firm, not loose, and does not crack. Finally, perform the coating operation. During the operation, ensure that the temperature is 18~26℃ and the humidity is ≤65%.

[0114] Table 8 Nutritional supplement formula ①

[0115]

[0116] (II) Preparation of Tablet Nutritional Supplements from the Nutritional Composition Provided in Example 6②

[0117] After mixing the ingredients with clear addition amounts in Table 9 and tableting excipients evenly, use 95% alcohol for granulation. Dry at 60℃ until the moisture content is below 5%, add the prepared granules, mix in an appropriate amount of microcrystalline cellulose and mix for 30 minutes. Add magnesium stearate and mix for 10 minutes, then discharge. Tablet, control the tablet weight (550 mg±27.5), ensure that the tablet surface is smooth and non-sticky, and the tablet is firm, not loose, and does not crack. Finally, perform the coating operation. During the operation, ensure that the temperature is 18~26℃ and the humidity is ≤65%.

[0118] Table 9 Nutritional supplement ② formula

[0119]

[0120] Example 20: Nutritional Supplement Effect Test

[0121] In order to study the effect of adding the nutritional supplement prepared by the present invention to the diet of young children on their nutritional status, especially on growth and development and trace element metabolism. In this embodiment, 20 children aged 6-12 with good dietary status were used as experimental subjects, and the two nutritional supplements prepared in Example 18 and the nutritional compositions prepared in Example 1 and Example 8 were directly added to the dietary soup for dissolution, with a daily dose of 2 servings, and the control group did not add nutritional supplements. After that, the height, weight, head circumference, chest circumference, and hip circumference of each group of children after consuming the nutritional composition and nutritional supplements for 3 months were measured, and the content of trace elements such as iron, copper, calcium, and zinc in serum was measured by atomic absorption spectrophotometry, and the chemiluminescence immunoassay was used to directly measure the level of growth hormone (HGH) in serum. The test results are shown in Tables 10 and 11.

[0122] Table 10 Changes in growth and development indicators in each group

[0123]

[0124] Table 11 Changes in serum zinc, iron, copper, calcium and magnesium concentrations in each group

[0125]

[0126] According to the data in Table 10, it can be seen that compared with the control, after adding the two nutritional compositions provided by the present invention and nutritional supplements ① and ②, the height, weight and HGH level of the children were significantly improved, especially the children supplemented with nutritional supplement ② had the most obvious improvement in various indicators, and there was no significant change in head circumference, chest circumference and hip circumference.

[0127] As can be seen from Table 11, after adding the two nutritional compositions and nutritional supplements ① and ②, the concentrations of copper, calcium and magnesium in the serum were significantly increased compared with the control, especially when nutritional supplement ② was added; the concentrations of zinc and iron changed slightly. The above results show that after supplementing nutritional supplements ① and ② during children's development, the content of trace elements in children's blood can be effectively increased and physical development can be promoted, among which nutritional supplement ② has a better effect.

[0128] The above is only a specific implementation of the present invention, but the design concept of the present invention is not limited thereto. Any non-substantial changes to the present invention using this concept shall be deemed as an infringement of the protection scope of the present invention.

Claims

1. A nutritional composition for promoting growth hormone secretion and thus promoting height growth, characterized in that: It comprises lysine, seaweed calcium and bovine colostrum basic protein, and the growth hormone comprises IGF-1 and HGH.

2. The nutritional composition according to claim 1, characterized in that Also included are glycine and glutamine.

3. The nutritional composition according to claim 2, characterized in that Also includes vitamin D and vitamin K2.

4. The nutritional composition according to claim 3, characterized in that The mass ratio of lysine, seaweed calcium, bovine colostrum basic protein, glycine, glutamine, vitamin D and vitamin K2 is (300-400): (50-100): (1-10): (10-20): (10-20): (0.001-0.004): (1-4).

5. A product that promotes the secretion of growth hormone and thus promotes growth, characterized in that: The nutritional composition comprises the nutritional composition according to any one of claims 1 to 4.

6. A composition for preparing an agent for increasing the level of somatomedin IGF-1, characterized in that: The composition comprises lysine, seaweed calcium and bovine colostrum basic protein.

7. A composition for preparing a reagent for increasing the level of growth hormone HGH, characterized in that: The composition comprises lysine, seaweed calcium and bovine colostrum basic protein.

8. Use of a composition for preparing an agent for increasing osteocalcin levels, characterized in that: The composition comprises lysine, seaweed calcium and bovine colostrum basic protein.

9. Use of a composition for preparing an agent for enhancing bone density, characterized in that: The composition comprises lysine, seaweed calcium and bovine colostrum basic protein.

10. Use of a composition for preparing an agent for reducing urine DPD levels, characterized in that: The composition comprises lysine, seaweed calcium and bovine colostrum basic protein.

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