Gingiva protecting composition as well as preparation method and application thereof

Abstract

The invention relates to the technical field of oral care, and particularly discloses a gingiva protecting composition as well as a preparation method and application thereof. The gingiva protecting composition comprises the following components: a spina date seed extract, an acanthopanax trifoliatus leaf and stem extract and an aloe barbadensis extract. The semen ziziphi spinosae extract, the acanthopanax trifoliatus leaf and stem extract and the aloe barbadensis extract in the gingival protecting composition have a synergistic effect on the free radical scavenging rate, and the formed gingival protecting composition can be added into an oral care product; the cell irritation of a common foaming agent sodium lauryl sulfate in oral care products such as toothpaste can be reduced, the problems of redness, pain, bleeding and the like of gingiva can be relieved, and the oral care product has the advantage of being good in taste.

Description

Technical Field

[0001] The present application relates to the field of oral care technology, and in particular to a gum protection composition and a preparation method and application thereof. Background Art

[0002] Swelling and aching of gum refers to the redness, swelling and pain of the gum tissue, which causes discomfort to the patient. It is generally a symptom of gingival inflammation, mainly due to poor oral hygiene, which leads to the deposition of dental plaque, tartar and soft plaque near the gingival margin. The most typical symptom is swollen gums, which may be accompanied by spontaneous bleeding or bleeding on probing.

[0003] Bad oral care habits, bad work and rest habits, and unhealthy diet may cause swollen and painful gums. In addition, toothpaste often contains irritating ingredients such as sodium lauryl sulfate, which can also cause irritation to the oral mucosa and gums, leading to swollen and painful gums.

[0004] At present, the commonly used anti-inflammatory drugs for treating swollen and painful gums include artificial bezoar metronidazole, tinidazole, ornidazole, amoxicillin, cephalosporin, ibuprofen capsules, pain-relieving tablets, etc. These drugs can help eliminate inflammation and relieve pain; but taking these drugs will cause a partial balance of oral flora. Long-term consumption of these drugs will increase the tolerance of pathogenic bacteria and reduce the efficacy of the drugs.

[0005] Currently, natural products and plants that can relieve gum problems include Sichuan pepper and ginger. Although Sichuan pepper can relieve gum problems through its antibacterial effect, it has a poor taste and can easily make consumers feel numb in the mouth; although ginger can relieve gum problems by reducing free radicals and reducing inflammation, it has a poor taste; Bazhen Soup can also be used to relieve gum problems. Bazhen Soup contains ingredients such as ginseng, Atractylodes macrocephala, White Poria cocos, Chinese angelica, Chuanxiong, White Peony, Rehmannia root, and Licorice. It has the effect of replenishing qi and blood, and can relieve symptoms of swollen and painful teeth as well as bleeding. However, Bazhen Soup may have some adverse reactions such as nausea and vomiting, and some consumers are allergic to Bazhen Soup or the medicinal materials in it.

[0006] Therefore, there is an urgent need to find a natural composition that can be added to toothpaste or mouthwash to protect gums, reduce gum irritation and have a good taste. Summary of the invention

[0007] The purpose of the present application is to overcome the deficiencies of the above-mentioned prior art and provide a gum protection composition and its preparation method and application. The gum protection composition of the present application has a synergistic effect on the free radical scavenging rate, can reduce the cell irritation of sodium lauryl sulfate, a commonly used foaming agent in toothpaste, and thus alleviate the redness, pain, bleeding and other problems of the gums.

[0008] To achieve the above purpose, the technical solution adopted by this application is:

[0009] The present application provides a gum protection composition, which comprises the following components:

[0010] Ziziphus jujuba seed extract, scutellaria baicalensis leaf stem extract and aloe barbadensis extract.

[0011] Ziziphus jujuba seed is a traditional Chinese medicinal material. The chemical components of Ziziphus jujuba seed mainly include flavonoids, saponins, alkaloids, etc. Flavonoid compounds are one of the main active ingredients in Ziziphus jujuba seed, and have anti-inflammatory and antioxidant effects. Saponin compounds, such as Ziziphus jujuba saponins A and B, are another important class of active ingredients in Ziziphus jujuba seed. They exert sedative and hypnotic effects by affecting the GABA and 5-HT systems, and Ziziphus jujuba saponin A has a certain effect on improving insomnia. Alkaloid compounds also account for a certain proportion in Ziziphus jujuba seed, and have anticonvulsant effects. Ziziphus jujuba seed has a variety of biological activities, including sedative and hypnotic, anti-anxiety, antioxidant and myocardial protection.

[0012] Aloe vera is a plant widely used in the cosmetics industry. Its chemical composition mainly includes polysaccharides, anthraquinone compounds, proteins, vitamins, minerals, etc. These ingredients make aloe vera have multiple functions in cosmetics, such as moisturizing, promoting wound healing, and anti-aging. The polysaccharides, amino acids and metal salts contained in aloe vera are the same as the components of natural moisturizing factors, and aloe vera has good moisturizing properties. The polysaccharides in aloe vera gel, such as mannan, can increase the activity of macrophages and promote wound healing. The ingredients contained in aloe vera, such as catalase, vitamin A, vitamin E and superoxide dismutase, can effectively eliminate free radicals and achieve the effect of delaying aging.

[0013] Bailuo is a plant that can be used as both medicine and food. Chinese data have long recorded it. Li Shizhen said: "Eupatorium wilfordii can treat rheumatism, strengthen muscles and bones, and its effect is profound"; "Guangdong Medicinal Plants Compendium" records that "its roots, stems and leaves can be used as medicine. The roots can dispel wind and dampness, eliminate blood stasis and relieve pain; the leaves can dispel wind, reduce swelling, relieve itching, and have the effects of relaxing muscles, activating blood circulation, reducing swelling and detoxifying." Bailuo contains a variety of chemical components such as chlorogenic acid, rutin, dicaffeoylquinic acid, polyphenol compounds, etc. Chlorogenic acid is a monocaffeoylquinic acid compound with a wide range of biological activities, such as antibacterial, antiviral, increasing white blood cells, protecting the liver and promoting bile secretion, anti-tumor, lowering blood pressure, lowering blood lipids, scavenging free radicals and exciting the central nervous system. Rutin is a flavonoid glycoside compound with anti-inflammatory, antiviral, aldose reductase inhibition, anti-radiation, anti-free radicals and vitamin P-like effects. Modern scientific research on the biological activity of chlorogenic acid has penetrated into many fields such as food, health care, medicine and daily chemical industry. Dicaffeoylquinic acid substances have the effects of promoting blood coagulation, lowering blood lipids, anti-inflammatory, antioxidant, anti-hepatitis and AIDS virus.

[0014] In the technical solution of the present application, the jujube seed extract, the white sedge leaf stem extract and the aloe vera extract have a synergistic effect on the free radical scavenging rate. The composed gum protection composition can be added to oral care products. It can not only reduce the cell irritation of oral care products such as sodium lauryl sulfate, a commonly used foaming agent in toothpaste, but also relieve the redness, pain, bleeding and other problems of the gums, and has the advantage of good taste.

[0015] As a preferred embodiment of the gum protection composition described in the present application, the mass ratio of the Ziziphus jujuba seed extract, the Ampelopsis pilosula leaf and stem extract and the Aloe vera extract in the gum protection composition is 1:(8-12):(1-3.33).

[0016] When the weight ratio of the extract of Ziziphus jujuba seed, the extract of the leaf stem of Ampelopsis pilosula and the extract of Aloe vera is 1:(8-12):(1-3.33), the DPPH free radical scavenging rate of the gum protection composition is better.

[0017] When green tea extract is used to replace the white balsam pear leaf and stem extract, the DPPH free radical scavenging rate of the gum protection composition constituted by the green tea extract is far lower than that of the gum protection composition of the present application.

[0018] When the gum protection composition does not contain two or one of the Chinese jujube seed extract, the white balsam pear leaf stem extract, and the aloe vera extract, the DPPH free radical scavenging rate of the gum protection composition is poor; when two of the Chinese jujube seed extract, the white balsam pear leaf stem extract, and the aloe vera extract are combined, there will be an antagonistic effect.

[0019] As a preferred embodiment of the gum protection composition described in the present application, the mass ratio of the Ziziphus jujuba seed extract, the Ampelopsis pilosula leaf and stem extract and the Aloe vera extract in the gum protection composition is 1:12:3.33.

[0020] The jujube seed extract, white balsam pear leaf stem extract, and aloe vera extract in the gum protection composition of the present application have a synergistic effect. The above-mentioned mass ratio has a better synergistic effect on the DPPH free radical scavenging rate. Applying it in oral care products can alleviate the irritation of the foaming agent sodium lauryl sulfate, thereby alleviating the redness, pain, bleeding and other problems of the gums.

[0021] As a preferred embodiment of the gum protection composition described in the present application, the preparation method of the extract of the leaf and stem of the white balsam pear comprises the following steps:

[0022] 1) Cut the leaf stems of the white balsam pear into small sections, add water to extract according to a solid-liquid ratio of 1:20, reflux for 2 hours, remove the residue, and obtain the first extract;

[0023] 2) taking the residue from step 1), adding water to extract at a solid-liquid ratio of 1:10, and refluxing for 1.5 hours to obtain a second extract;

[0024] 3) The first extract of step 1) and the second extract of step 2) are combined, centrifuged to remove the residue, the centrifuged liquid is filtered again, the filtrate is retained, concentrated and dried to obtain the white sedge leaf stem extract.

[0025] In some specific embodiments, the filtration includes ceramic membrane filtration and ultrafiltration membrane filtration.

[0026] The centrifuged liquid is filtered through a ceramic membrane, the retentate is discarded, and the filtrate is retained; the centrifuged liquid is then filtered through an ultrafiltration membrane, the retentate is discarded, and the filtrate is retained.

[0027] The extraction process is used to prepare the leaf and stem extract of the white balsam pear, which does not use volatile organic solvents, has little impact on the environment, and is relatively safe to the human body; the operation is simple and easy to control; water can be used as a polar solvent to effectively extract active ingredients with strong polarity, such as polysaccharides, flavonoids, etc.

[0028] As a preferred embodiment of the gum protection composition described in the present application, the preparation method of the spiny jujube seed extract comprises the following steps:

[0029] The spiny jujube kernels are crushed, 6 to 8 times the amount of water are added for the first extraction, and then 5 to 7 times the amount of water are added for the second extraction, and then the extract is concentrated and spray-dried, with the inlet air temperature being 160 to 195° C. and the outlet air temperature being 60 to 90° C., to obtain the spiny jujube kernel extract;

[0030] The temperature of the first and second extractions are both 80-100° C., and the extraction time is both 1.5-2.5 h. Preferably, the temperature of the first and second extractions are both 90° C., and the extraction time is both 2 h.

[0031] As a preferred embodiment of the gum protection composition described in the present application, the polysaccharide content of the aloe vera extract is greater than 6000 mg / 100 g, and the O-acetyl content is greater than 4000 mg / 100 g.

[0032] As a preferred embodiment of the gum protection composition described in the present application, the gum protection composition further includes a preservative; the preservative includes at least one of 1,2-hexanediol, 1,2-pentanediol, and caprylyl glycol.

[0033] The present application also provides a method for preparing the above-mentioned gum protection composition, comprising the following steps:

[0034] A preservative is added into water to obtain a mixed solution, and then the spiny jujube seed extract, the white balsam pear leaf stem extract and the aloe vera extract are added into the mixed solution and stirred evenly to prepare a gum protection composition.

[0035] The present application also provides a toothpaste or a mouthwash, wherein the toothpaste or the mouthwash comprises the gum protection composition.

[0036] In some specific embodiments, the toothpaste includes phase A, phase B, phase C, phase D, phase E and phase F;

[0037] The phase A comprises a sorbitol solution;

[0038] Phase B includes glycerin, Chondrus Crispus extract and cellulose gum;

[0039] Phase C includes water, sodium monofluorophosphate, sodium benzoate, PEG-400, stevia (STEVIA REBAUDIANA) leaf / stem extract and the above-mentioned gum protection composition;

[0040] Phase D includes silicon dioxide;

[0041] Phase E includes sodium lauryl sulfate;

[0042] Phase F includes fragrance;

[0043] The preparation method of the toothpaste comprises the following steps:

[0044] 1. Take sorbitol solution and add it into the batching tank;

[0045] 2. Mix phase B evenly until there is no lumps, add it to the sorbitol solution, and stir for 5 to 10 minutes;

[0046] 3. Mix phase C evenly until completely dissolved, add it to the batching tank, and stir for 3 to 5 minutes;

[0047] 4. Add phase D into the batching tank twice, vacuum -0.09MPa each time, and stir for 5 to 10 minutes;

[0048] 5. Add phase E into the batching tank, vacuum -0.09MPa, and stir for 5 to 10 minutes;

[0049] 6. Add phase F into the batching tank, vacuum to -0.09MPa, and stir for 10 to 15 minutes.

[0050] The present application also provides the use of the gum protection composition in the preparation of oral care products.

[0051] As a preferred embodiment of the application described in the present application, the oral care product includes toothpaste or mouthwash.

[0052] The gum protection composition of the present application is applied to oral care products, which can not only alleviate the irritation of the foaming agent sodium lauryl sulfate, but also relieve gum bleeding, gum pain, and gum redness.

[0053] Compared with the prior art, this application has the following beneficial effects:

[0054] The present application provides a gum protection composition and its preparation method and application. The present application adopts a jujube seed extract, a white sedge leaf stem extract, and an aloe vera extract to have a synergistic effect on the free radical scavenging rate. The gum protection composition of the present application is used in oral care products, which not only has the advantage of reducing the cell irritation of sodium lauryl sulfate, a common foaming agent in toothpaste, but also can alleviate the redness, pain, bleeding and other problems of the gums, and has the advantage of good taste. BRIEF DESCRIPTION OF THE DRAWINGS

[0055] Figure 1 This is a graph showing the DPPH free radical scavenging test results of Experimental Example 1;

[0056] Figure 2 This is the MTT test result diagram of Experimental Example 3 (##: compared with the model group, the cell viability of Example 1 at an action concentration of 1% was extremely significantly increased (P<0.01); *: compared with Example 1, the cell viability of Comparative Example 1 at an action concentration of 1% was significantly decreased (P<0.05). DETAILED DESCRIPTION

[0057] In order to better illustrate the purpose, technical solutions and advantages of the present application, the present application will be further described below in conjunction with the accompanying drawings and specific embodiments.

[0058] In the following examples and comparative examples, the experimental methods used are conventional methods unless otherwise specified, and the materials, reagents, etc. used are all commercially available unless otherwise specified, and the components and raw materials used in each parallel experiment are of the same type.

[0059] In the following examples and comparative examples:

[0060] The preparation method of the bracteatum leaf and stem extract comprises the following steps:

[0061] 1) Cut the leaf stems of the white balsam pear into small sections of 1 to 3 cm, add water to extract according to a solid-liquid ratio of 1:20, reflux for 2 hours, remove the residue, and obtain the first extract;

[0062] 2) taking the residue from step 1), adding water to extract at a solid-liquid ratio of 1:10, and refluxing for 1.5 hours to obtain a second extract;

[0063] 3) The first extract of step 1) and the second extract of step 2) are combined, centrifuged (12000 rpm, 30 minutes) to remove the residue, the centrifuged liquid is filtered using a ceramic membrane (0.2 μm), the retentate is discarded, and the filtrate is retained; the filtrate is filtered using an ultrafiltration membrane (100,000 Daltons), the retentate is discarded, and the filtrate is retained; the filtrate is concentrated; the filtrate is cooled to room temperature, centrifuged (12000 rpm, 30 minutes) to remove the residue; and then spray-dried to obtain the white calamus leaf stem extract.

[0064] The extract of white balsam pear leaf and stem can also be obtained by conventional extraction methods.

[0065] The preparation method of the Chinese date seed extract comprises the following steps:

[0066] The spiny jujube kernels were crushed, 7 times the amount of water was added for the first extraction, and then 6 times the amount of water was added for the second extraction, and then the extract was concentrated and spray-dried, with the inlet air temperature of 180°C and the outlet air temperature of 75°C to obtain the spiny jujube kernel extract;

[0067] The temperature for the first and second extractions was 90°C, and the extraction time was 2 h.

[0068] The Chinese jujube seed extract can be obtained by the above-mentioned preparation method, or can be purchased from Guangdong Yifang Pharmaceutical Co., Ltd.

[0069] Aloe vera extract was sourced from Yunnan Wanlv Biological Co., Ltd.;

[0070] Green tea extract was sourced from Guangzhou Luojie Biotechnology Co., Ltd.;

[0071] 1,2-Hexanediol was obtained from Thor Specialty Chemicals (Zhenjiang) Co., Ltd.

[0072] Embodiments 1 to 5: A gum protection composition and a preparation method thereof

[0073] Embodiments 1 to 5 provide a gum protection composition comprising the following components:

[0074] Ziziphus jujuba seed extract, scutellaria baicalensis leaf stem extract, aloe barbadensis extract, 1,2-hexanediol and water.

[0075] The specific formulas of the gum protection compositions of Examples 1 to 5 are shown in Table 1.

[0076] The preparation method of the gum protection composition comprises the following steps:

[0077] First, add water to a beaker, then add 1,2-hexanediol to the beaker and stir evenly; then add the white balsam pear leaf stem extract, the spiny jujube seed extract, and the aloe vera extract to the beaker and stir evenly.

[0078] Table 1

[0079]

[0080] Comparative Examples 1 to 10

[0081] Comparative Examples 1 to 10 also provide a gum protection composition, and the specific formula is shown in Table 2.

[0082] The preparation methods of the gum protection compositions of Comparative Examples 1-10 are similar to those of Examples 1-5.

[0083] Table 2

[0084]

[0085]

[0086] Test Example 1: DPPH free radical scavenging ability of gum protection composition

[0087] Free radicals are a class of highly reactive chemical species that contain unpaired electrons, which makes them very unstable and prone to react with other molecules.

[0088] Free radicals may cause gum problems through the following mechanisms: ① Oxidative stress. Free radicals can cause oxidative stress. When the body's antioxidant system cannot neutralize excessive free radicals, it will cause cell damage, which may aggravate gum inflammation; ② Cell damage. Free radical damage to cell membranes may cause cells to release inflammatory mediators, such as prostaglandins and interleukins, which can promote the aggregation and activation of inflammatory cells and enhance the inflammatory response. ③ Periodontal tissue damage: Free radicals may directly damage periodontal tissues, including gums and periodontal ligaments, leading to swollen and painful gums. Therefore, the gum protection effect of the composition can be studied by studying the anti-free radical properties of the composition.

[0089] DPPH free radical is a very stable free radical with oxygen center. It is one of the important indicators of the antioxidant capacity of the sample and is widely used in antioxidant foods. DPPH free radical has a single electron, its alcohol solution is purple, and has strong absorption at 515nm. When antioxidants are present, DPPH free radicals are scavenged, the color of its solution becomes lighter, and the absorbance at 515nm decreases. Within a certain range, the change in its absorbance is proportional to the degree of free radical scavenging. The degree of absorbance decrease reflects the ability of the sample to scavenge DPPH free radicals.

[0090] Test method:

[0091] 0.12mg / mL DPPH ethanol solution: Accurately weigh 0.012g of 2,2-biphenyl-1-picrylhydrazyl and dilute to 100mL with 95% ethanol, and keep away from light for later use. Weigh ascorbic acid powder and dissolve and dilute to 0.025mg / mL, 0.02mg / mL, 0.015mg / mL, 0.01mg / mL, 0.005mg / mL concentration gradients with 95% ethanol to verify the experimental system. Use a pipette to transfer 100μL of each sample (the gingival protection composition of Examples 1 to 5 and Comparative Examples 1 to 10) and dilute to 10mL respectively.

[0092] Use 10mL test tubes to set up sample tubes (T), sample background (T0), DPPH tubes (C) and solvent background (C0). Three parallel tubes need to be set up for each sample sample tube (T), and three parallel tubes need to be set up for the DPPH tube (C). Add 1mL of sample solution of the same concentration to each sample tube (T) and sample background (T0). Add water to all test tubes (T, T0, C, C0) to 3mL and mix well. Add 1mL of DPPH ethanol solution to the sample tube (T) and DPPH tube (C), replace the sample background (T0) and solvent background (C0) with 95% ethanol, shake gently, and let stand at room temperature for 5 minutes. Transfer each reaction solution into a 1cm cuvette and measure the absorbance at 517nm. Calculate the DPPH free radical scavenging rate:

[0093]

[0094] The results are shown in Table 3.

[0095] Table 3 DPPH free radical scavenging rate

[0096] Group DPPH clearance rate% (mean ± standard error of the mean) Example 1 83.19±1.34 Example 2 81.10±0.93 Example 3 83.95±0.67 Example 4 75.22±0.69 Example 5 88.64±0.56 Comparative Example 1 27.60±0.35 Comparative Example 2 29.46±1.49 Comparative Example 3 14.74±1.47 Comparative Example 4 11.06±0.09 Comparative Example 5 30.57±0.37 Comparative Example 6 29.87±0.43 Comparative Example 7 21.10±0.72 Comparative Example 8 48.02±1.07 Comparative Example 9 57.22±0.96 Comparative Example 10 30.18±1.60

[0097] The DPPH free radical scavenging test results are shown in Table 3 and Figure 1 The gum protection compositions prepared in Examples 1 to 5 all have a high DPPH free radical scavenging rate, wherein when the weight ratio of the jujube seed extract, the white sedge leaf stem extract, and the aloe vera extract is 1:(8-12):(1-3.33), the DPPH free radical scavenging rate of the gum protection composition is better.

[0098] When green tea extract is used to replace the balsam pear leaf and stem extract, the DPPH free radical scavenging rate of the gum protection composition constituted thereby is far lower than that of Examples 1 to 5 of the present application.

[0099] The gum protection compositions of Comparative Examples 2 to 7 do not contain two or one of the extract of Ziziphus jujuba seed, the extract of the leaf stem of Ampelopsis pilosula, and the extract of Aloe vera, and the DPPH free radical scavenging rate of the gum protection compositions is lower than that of Examples 1 to 5 of the present application.

[0100] In Comparative Examples 8 to 10, the weight ratio of the Ziziphus jujuba seed extract, the Bletilla striata leaf stem extract, and the Aloe vera extract is not within the range of 1:(8-12):(1-3.33), and the DPPH radical scavenging rate of the prepared gum protection composition is lower than that of the gum protection composition of Examples 1 to 5.

[0101] Test Example 2: Synergistic Effect Experiment of Gum Protecting Composition

[0102] Calculation of synergy index:

[0103] Combination Index (CI): CI is an important parameter to measure whether the combination of compounds has synergistic or antagonistic effects. The calculation formula of CI index is:

[0104]

[0105] Wherein C1, C2, C3 are the free radical scavenging rates of components 1, 2, 3 acting alone, respectively; C123 represents the corresponding free radical scavenging rates when the components are compounded into a composite.

[0106] After calculating the CI value, the synergy produced by the combination can be interpreted according to its size:

[0107] (1) If CI < 1, it indicates a synergistic effect, which means that the combination of ingredients has a stronger or more significant effect on improving the target physiological process or therapeutic effectiveness than each ingredient alone.

[0108] (2) If CI = 1, it means that there is no interaction, which means that there is no additional gain or loss between the components.

[0109] (3) If CI>1, it indicates an antagonistic effect, which means that the combination of ingredients shows a lower level or weaker significant improvement in the target physiological process or therapeutic effectiveness than when each ingredient is used alone.

[0110] Table 4 Synergy index

[0111]

[0112]

[0113] The calculation results of the synergy effect index are shown in Table 4.

[0114] The synergistic effect index (CI) of the gum protection composition prepared in Examples 1 to 5 is less than 1, indicating that the Ziziphus jujuba seed extract, the Ampelopsis pilosula leaf stem extract, and the Aloe barbadensis extract have a synergistic effect, among which when the Ziziphus jujuba seed extract, the Ampelopsis pilosula leaf stem extract, and the Aloe barbadensis extract are in a weight ratio of 1:(8-12):(1-3.33), the synergistic effect of the above three extracts is better.

[0115] When two of the extract of Ziziphus jujuba seed, the extract of the leaf and stem of Ampelopsis pilosula, and the extract of Aloe vera were combined, namely, comparative examples 5 to 7, the synergistic effect index (CI) was greater than 1, indicating an antagonistic effect.

[0116] When the weight ratio of the extract of Ziziphus jujuba seed, the extract of the leaf stem of Ampelopsis pilosula and the extract of Aloe vera is not within the range of 1:(8-12):(1-3.33), the synergistic effect index (CI) is greater than 1, indicating an antagonistic effect.

[0117] Experimental Example 3: Human oral squamous cell carcinoma cell viability test

[0118] Human oral squamous cell carcinoma cells (HSC-3) were used to test the cell viability of different gum protection compositions (Example 1 and Comparative Example 1). The greater the cell viability, the less irritating the gum protection composition is to the gingival epithelial cells and the less irritating it is to the gums.

[0119] HSC-3 cells were taken as the research object, and sodium lauryl sulfate (SDS) was used as the model group. After SDS stimulation modeling and adding the test samples (the gingival protection compositions prepared in Example 1 and Comparative Example 1), the protective effect of the test samples on the cells after SDS stimulation was evaluated by detecting the cell viability.

[0120] The MTT method is a method for detecting cell survival and growth. The detection principle is that the succinate dehydrogenase in the mitochondria of living cells can reduce exogenous MTT to water-insoluble blue-purple crystalline formazan and deposit it in the cells, while dead cells do not have this function. Dimethyl sulfoxide (DMSO) can dissolve the formazan in the cells, and its light absorption value is measured at a wavelength of 570nm using an enzyme-linked immunosorbent assay, which can indirectly reflect the number of living cells. Within a certain range of cell numbers, the amount of MTT crystals formed is proportional to the number of cells.

[0121] Test method:

[0122] (1) Cell inoculation: Oral squamous epithelial cells HSC-3 in the logarithmic growth phase were selected and routinely digested with 1 mL of 0.25% trypsin. 3 mL of complete culture medium was immediately added to resuspend the cells. After pipetting and mixing, the cells were counted and the cell concentration was adjusted to 8 × 10 4 The cells were inoculated at a density of 100 μL / mL in a 96-well plate, with 100 μL of cell solution per well, and incubated overnight in an incubator (37° C., 5% CO 2 ).

[0123] (2) Experimental grouping: The experiment set up a blank control group, a positive control group and a sample group. In the sample group, each sample was set at one concentration, and 6 replicate wells were set under each concentration gradient.

[0124] (3) Solution preparation: Prepare working solutions of the test substance of different concentrations according to the experimental design (Table 5).

[0125] Table 5 Experimental design

[0126]

[0127] (4) Drug administration: According to the specific experimental design in Table 3, when the cell plating rate in the 96-well plate reaches 50% to 60%, group drug administration is carried out, and the dosage per well is 100 μL, and each group has 6 replicate wells; zero wells are set without cell inoculation, and only 100 μL PBS is added. After drug administration, the 96-well plate is placed in an incubator (37° C., 5% CO2) for incubation.

[0128] (5) Detection: After the cells were incubated overnight, 10 μL of 5 mg / mL MTT solution was added to each well. After incubation at 37°C in the dark for 3–4 h, the supernatant was discarded and 150 μL of DMSO was added to each well. The cells were shaken for 5–10 min to allow the crystals to fully dissolve. The OD value of each well was then measured on a microplate reader at a wavelength of 570 nm.

[0129] (6) Calculation of relative cell viability: Calculated according to the formula, cell viability (%) = [(OD value of sample well - OD value of zero-adjusting well) / (OD value of blank well - OD value of zero-adjusting well)] × 100%.

[0130] Test results:

[0131] Each sample was administered at one concentration, and a cell viability test was performed on HSC-3 cells. The results of the cell viability test are shown in Table 6 below. Figure 2 . The experiment was set up with a model group (SDS). Compared with the blank group, the cell viability of the modeling group decreased extremely significantly (P<0.01), indicating that the stimulation conditions of this experiment were effective. The experiment was set up with a positive control group (dipotassium glycyrrhizinate), and the cell viability of the positive control group was extremely significantly increased compared with the model group (P<0.01), and the experimental system was considered effective. According to the MTT test results, under the stimulation of 80μg / mL SDS, compared with the model group, the cell viability of Example 1 was extremely significantly increased when the action concentration was 1% (P<0.01); compared with Comparative Example 1, the cell viability of Example 1 was significantly increased when the action concentration was 1% (P<0.05). Example 1 can alleviate the irritation caused by SDS.

[0132] Table 6 MTT test results

[0133]

[0134] Test Example 4: Consumer Testing

[0135] In order to further verify the use effect of the gum protection composition, Example 1 was added to toothpaste products to conduct consumer research. The test formula of gum protection toothpaste is shown in Table 7.

[0136] Preparation method of toothpaste: 1. Take sorbitol solution and add it into the mixing tank;

[0137] 2. Mix phase B evenly until there is no lumps, add it to the sorbitol solution, and stir for 5 to 10 minutes;

[0138] 3. Mix phase C evenly until completely dissolved, add it to the batching tank, and stir for 3 to 5 minutes;

[0139] 4. Add phase D into the batching tank twice, vacuum -0.09MPa each time, and stir for 5 to 10 minutes;

[0140] 5. Add phase E into the batching tank, vacuum -0.09MPa, and stir for 5 to 10 minutes;

[0141] 6. Add phase F into the batching tank, vacuum to -0.09MPa, and stir for 10 to 15 minutes.

[0142] Table 7 Toothpaste formula

[0143]

[0144]

[0145] Subject screening: Information about the subjects is obtained by asking about the situation in the past week, and the examiner will score the subjects accordingly based on their answers. Eligible subjects should meet one of the following conditions: ① Occasional bleeding (less than three times a week) or small amount of bleeding when biting hard objects or brushing teeth; ② Discomfort or mild pain when eating or brushing teeth.

[0146] Each group participated in the consumer survey for a total of 30 days. The subjects used toothpaste twice a day, brushing their teeth for at least 3 minutes each time. After 28 days of use, the subjects were asked about their subjective feelings.

[0147] Regarding the degree of agreement with relieving gingival bleeding, select: Strongly agree, Agree, Disagree, Strongly Disagree.

[0148] The degree of agreement regarding relieving gum pain was: Strongly agree, Agree, Disagree, Strongly Disagree.

[0149] Regarding the degree of agreement with relieving gum redness, select: Strongly agree, Agree, Disagree, Strongly Disagree.

[0150] Choices for acceptance of taste: Very accept, Accept, Do not accept, Very do not accept.

[0151] Test results:

[0152] 1. Degree of recognition for relieving gingival bleeding:

[0153] Table 8

[0154]

[0155] 2. Degree of recognition for relieving gum pain:

[0156] Table 9

[0157]

[0158] 3. Degree of recognition for relieving gingival redness:

[0159] Table 10

[0160]

[0161]

[0162] 4. Acceptance of taste:

[0163] Table 11

[0164]

[0165] According to the records in Tables 8 to 11, compared with toothpastes not containing the gum protection composition of Example 1, the toothpaste containing the gum protection composition of Example 1 has significantly improved the recognition degree of relieving gum bleeding, the recognition degree of relieving gum pain, the recognition degree of relieving gum redness, etc. In addition, the consumer acceptance of the toothpaste containing the gum protection composition of Example 1 is as high as 80%, which is equivalent to the toothpaste not containing the gum protection composition of Example 1.

[0166] The gum protection composition containing jujube seed extract, white balsam pear leaf stem extract and aloe vera extract can reduce the cell irritation of sodium lauryl sulfate, a common foaming agent in toothpaste. The gum protection composition of the present application can relieve the problems of redness, pain, bleeding and the like of gums.

[0167] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present application rather than to limit the scope of protection of the present application. Although the present application has been described in detail with reference to the preferred embodiments, ordinary technicians in this field should understand that the technical solution of the present application can be modified or replaced by equivalents without departing from the essence and scope of the technical solution of the present application.

Claims

1. A gum protection composition, characterized in that: The gum protection composition comprises the following components: Ziziphus jujuba seed extract, scutellaria baicalensis leaf stem extract and aloe barbadensis extract.

2. The gum protection composition according to claim 1, characterized in that The mass ratio of the Ziziphus jujuba seed extract, the Bletilla striata leaf and stem extract and the Aloe vera extract in the gum protection composition is 1:(8-12):(1-3.33).

3. The gum protection composition according to claim 2, characterized in that: The mass ratio of the Ziziphus jujuba seed extract, the Bletilla striata leaf and stem extract and the Aloe vera extract in the gum protection composition is 1:12:3.

33.

4. The gum protection composition according to claim 1, characterized in that: The preparation method of the bracteatum leaf and stem extract comprises the following steps: 1) Cut the leaf stems of the white balsam pear into small sections, add water to extract according to a solid-liquid ratio of 1:20, reflux for 2 hours, remove the residue, and obtain the first extract; 2) taking the residue from step 1), adding water to extract at a solid-liquid ratio of 1:10, and refluxing for 1.5 hours to obtain a second extract; 3) The first extract of step 1) and the second extract of step 2) are combined, centrifuged to remove the residue, the centrifuged liquid is filtered again, the filtrate is retained, concentrated and dried to obtain the white sedge leaf stem extract.

5. The gum protection composition according to claim 1, characterized in that: The preparation method of the Chinese date seed extract comprises the following steps: The spiny jujube kernels are crushed, 6 to 8 times the amount of water are added for the first extraction, and then 5 to 7 times the amount of water are added for the second extraction, and then the extract is concentrated and spray-dried, with the inlet air temperature being 160 to 195° C. and the outlet air temperature being 60 to 90° C., to obtain the spiny jujube kernel extract; The temperatures for the first and second extractions were both 80-100°C, and the extraction times were both 1.5-2.5h.

6. The gum protection composition according to claim 1, characterized in that: The gum protection composition further comprises an antiseptic; the antiseptic comprises at least one of 1,2-hexanediol, 1,2-pentanediol and caprylyl glycol.

7. The method for preparing the gum protection composition according to any one of claims 1 to 6, characterized in that: The following steps are involved: A preservative is added into water to obtain a mixed solution, and then the spiny jujube seed extract, the white balsam pear leaf stem extract and the aloe vera extract are added into the mixed solution and stirred evenly to prepare a gum protection composition.

8. A toothpaste or mouthwash, characterized in that: The toothpaste or mouthwash comprises the gum protection composition according to any one of claims 1 to 6.

9. Use of the gum protection composition according to any one of claims 1 to 6 in the preparation of oral care products.

10. The use according to claim 9, characterized in that The oral care product includes toothpaste or mouthwash.

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