A salmon facial mask with anti-inflammatory, anti-aging and repairing effects and preparation method thereof

Abstract

The present invention provides a salmon mask with anti-inflammatory, anti-aging and repairing effects and a preparation method thereof, belonging to the field of cosmetic technology. The salmon mask includes the following components by weight: Phase A: 2-5 parts of aloe vera gel, 1-3 parts of sodium carrageenan, 2-4 parts of sodium alginate, 1-5 parts of konjac gum, 3-5 parts of glycerol and 85-95 parts of high-purity water; Phase B: 5-10 parts of polydeoxyribonucleotides, 10-25 parts of Centella asiatica extract, 10-25 parts of Portulaca oleracea extract, 5-15 parts of Calendula officinalis extract, 10-20 parts of hyaluronic acid, 2-5 parts of acetyl tetrapeptide-15, 1-3 parts of palmitoyl tetrapeptide-7, 1-3 parts of acetyl hexapeptide-8, 3-8 parts of conopeptide, 1-3 parts of snake venom tripeptide and 2-5 parts of pentapeptide-3. The present invention makes the obtained mask have better anti-inflammatory, moisturizing and repairing effects by selecting specific ingredients.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetics, and particularly relates to a salmon facial mask with anti-inflammatory, anti-aging and repairing effects and a preparation method thereof. Background Art

[0002] With the improvement of the quality of life, people's awareness of facial skin care has gradually increased, and a series of beauty and skin care products have emerged. Due to the advantages of complete functions and easy to carry, the consumption of facial masks has gradually increased, and its development should not be underestimated. As a type of facial mask, gel masks have developed rapidly in recent years. At present, the gel masks on the market mainly have antioxidant, moisturizing and anti-aging effects, and the green and pollution-free functional gel masks developed with natural active ingredients are even more popular among consumers.

[0003] There are four main types of facial masks: mud paste, tear-off, gel, and wet wipes. Common mud paste masks include seaweed masks and mineral mud masks. The most common tear-off mask is a nose patch for blackheads and acne. The gel type is most famous for sleeping masks. The wet wipes are generally single-piece mask papers soaked with beauty liquid. However, mud paste masks need to be applied layer by layer on the face and washed with water after use, which is very inconvenient to use. The wet wipes type non-woven fabric impregnated with nutrient solution needs to be taken out of the packaging bag when used. The impregnated nutrient solution drips and contaminates other items, making it inconvenient to use. Secondly, the non-woven fabric impregnated with nutrient solution has poor adhesion and is easy to curl up or fall off the face. Furthermore, the ability of a thin layer of non-woven fabric to impregnate nutrient solution is limited, and some nutrients or active ingredients are difficult to adhere to the non-woven fabric.

[0004] Polymer hydrogel is a type of polymer with a three-dimensional network structure. It is widely used in bioengineering, medical care, and beauty due to its unique hydrophilicity, water retention, and good biocompatibility. Hydrogel mask uses hydrophilic gel as the mask matrix. It is rich in a large number of nutrients and moisture required by the skin, has strong adhesion, and is non-irritating to the skin. It has the following effects: deep hydration and nutrition, accelerates metabolism; improves skin roughness, dullness, fades deposited pigments, fades black and yellow spots; hydrates the skin stratum corneum, activates cells, reduces fine lines, and tightens the skin.

[0005] As disclosed in Chinese patent CN109453072A, an anti-aging gel mask nourished by wolfberry and a preparation method thereof, the gel mask includes a gel matrix, a mask liquid I and a mask liquid II. The preparation method of the gel mask comprises the following steps: weighing an initiator and putting it into a reactor, adding high-purity water to dissolve, adding N-isopropyl acrylamide monomer and stirring until dissolved, adding potassium chloride and stirring evenly, then adding konjac gum and locust bean gum and stirring evenly to obtain a suspension; adding a cross-linking agent to obtain a mixed solution, immersing it in the mask liquid I and prepolymerizing it first, then continuing to soak and remove impurity ions after the freezing polymerization reaction is completed, and then placing it in a freeze drying box to dry, and then immersing it in the mask liquid II and injecting it into a mask-shaped mold to obtain an anti-aging gel mask nourished by wolfberry. The mask of the invention mainly forms a covering film on the skin, which can remove grease and dirt accumulated in the pores, supplement skin nutrition, condition the skin, smooth fine lines, and delay aging.

[0006] Another example is Chinese patent CN103520070A, which discloses a highly elastic, unsupported, environmentally friendly gel mask and its preparation method. The gel mask is polymerized by dissolving olefin monomer M1, biopolymer M2, chemical crosslinker M3, physical crosslinker M4, plant essential oil, whitening ingredients and anti-aging active ingredients in water. The preparation method includes: 1) preparation of aqueous solutions of M1, M2, M3 and M4; 2) preparation of essence; 3) mixing of prepolymer solution; 4) preparation of gel mask; 5) packaging of gel mask. The gel mask prepared by the method has moderate viscosity, good elasticity, good ductility and toughness, does not need to be laminated with non-woven fabric to improve the mechanical strength of the gel mask, is easy to carry, has adjustable thickness, and can be reused. The viscoelasticity, skin affinity and moisturizing properties of the mask are adjusted by adjusting the monomer concentration, feed ratio, whitening and anti-aging active ingredients, reaction temperature, water content and feeding order. The gel mask of the invention can be used in the fields of skin care, moisturizing, beauty, anti-aging, etc.

[0007] However, although the existing facial masks have good hydrating and moisturizing properties and anti-aging effects, their anti-inflammatory and repairing effects still cannot better meet the needs. Therefore, it is necessary to develop a salmon facial mask with anti-inflammatory, anti-aging and repairing effects and a preparation method thereof. Summary of the invention

[0008] Based on the deficiencies in the prior art, the present invention aims to provide a salmon facial mask with anti-inflammatory, anti-aging and repairing effects and a preparation method thereof. The present invention selects specific ingredients to enable the obtained facial mask to have better anti-inflammatory, moisturizing and repairing effects.

[0009] In order to achieve the above object, the present invention adopts the following technical solution:

[0010] A salmon facial mask with anti-inflammatory, anti-aging and repairing effects, comprising the following components in parts by weight:

[0011] Phase A: 2-5 parts of aloe vera gel, 1-3 parts of sodium carrageenan, 2-4 parts of sodium alginate, 1-5 parts of konjac gum, 3-5 parts of glycerin and 75-95 parts of high-purity water;

[0012] Phase B: 5-10 parts of polydeoxyribonucleotides, 10-25 parts of Centella asiatica extract, 10-25 parts of Portulaca oleracea extract, 5-15 parts of Calendula officinalis extract, 10-20 parts of hyaluronic acid, 2-5 parts of acetyl tetrapeptide-15, 1-3 parts of palmitoyl tetrapeptide-7, 1-3 parts of acetyl hexapeptide-8, 3-8 parts of cono peptide, 1-3 parts of snake venom tripeptide and 2-5 parts of pentapeptide-3.

[0013] Preferably, the salmon facial mask comprises the following components in parts by weight:

[0014] Phase A: 3-4 parts of aloe vera gel, 1.5-2.5 parts of sodium carrageenan, 2.5-3.5 parts of sodium alginate, 2-4 parts of konjac gum, 4-5 parts of glycerin and 78-92 parts of high-purity water;

[0015] Phase B: 6-8 parts of polydeoxyribonucleotides, 15-20 parts of Centella asiatica extract, 15-20 parts of Portulaca oleracea extract, 8-12 parts of Calendula officinalis extract, 12-18 parts of hyaluronic acid, 3-4 parts of acetyl tetrapeptide-15, 1.5-2.5 parts of palmitoyl tetrapeptide-7, 1.5-2.5 parts of acetyl hexapeptide-8, 4-6 parts of cono peptide, 1.5-2.5 parts of snake venom tripeptide and 3-4 parts of pentapeptide-3.

[0016] The above polydeoxyribonucleotides are salmon-derived polydeoxyribonucleotides purchased from Xi'an Wanlv Biotechnology Co., Ltd.

[0017] As some preferred implementation schemes, the present invention controls the mass ratio of aloe vera gel, sodium carrageenan, sodium alginate and konjac gum to 2-5:1-3:2-4:1-5, which significantly improves the film-forming properties of the facial mask; preferably, the mass ratio of aloe vera gel, sodium carrageenan, sodium alginate and konjac gum is controlled to 4:2:3:4, so that the transparency, spreadability and uniformity of the facial mask are significantly improved.

[0018] As some preferred embodiments, the mass ratio of the Centella asiatica extract, the Portulaca oleracea extract and the Calendula officinalis extract is 0.5-5:0.5-5:1;

[0019] Preferably, the mass ratio of the Centella asiatica extract, the Portulaca oleracea extract and the Calendula officinalis extract is 1-3:1-3:1;

[0020] More preferably, the mass ratio of the Centella asiatica extract, the Portulaca oleracea extract and the Calendula officinalis extract is 1.8:1.8:1.

[0021] During the implementation of the present invention, it was found that by adding Centella asiatica extract, Portulaca oleracea extract and Calendula officinalis extract to the components and controlling the mass ratio of the three, not only the anti-inflammatory and repairing effects of the facial mask are improved, but also the facial mask has the effects of moisturizing, anti-oxidation, increasing skin elasticity and repairing.

[0022] As other preferred embodiments, the mass ratio of acetyl tetrapeptide-15, palmitoyl tetrapeptide-7, acetyl hexapeptide-8, snake venom tripeptide and pentapeptide-3 is 1-5:1:1:1:1-5;

[0023] Preferably, the mass ratio of acetyl tetrapeptide-15, palmitoyl tetrapeptide-7, acetyl hexapeptide-8, snake venom-like tripeptide and pentapeptide-3 is 2:1:1:1:2.

[0024] The present invention adds acetyl tetrapeptide-15, palmitoyl tetrapeptide-7, acetyl hexapeptide-8, snake venom tripeptide and pentapeptide-3 to the components, so that the anti-inflammatory effect of the mask is significantly enhanced, and the water retention and anti-aging effects of the mask are also significantly improved.

[0025] As a preferred embodiment, the salmon facial mask comprises the following components in parts by weight:

[0026] Phase A: 4 parts of aloe vera gel, 2 parts of sodium carrageenan, 3 parts of sodium alginate, 4 parts of konjac gum, 5 parts of glycerin and 82 parts of high-purity water;

[0027] Phase B: 8 parts of polydeoxyribonucleotides, 18 parts of Centella asiatica extract, 18 parts of Portulaca oleracea extract, 10 parts of Calendula officinalis extract, 15 parts of hyaluronic acid, 4 parts of acetyl tetrapeptide-15, 2 parts of palmitoyl tetrapeptide-7, 2 parts of acetyl hexapeptide-8, 5 parts of cono peptide, 2 parts of snake venom tripeptide and 4 parts of pentapeptide-3.

[0028] The present invention adds the following ingredients into the facial mask:

[0029] Polydeoxyribonucleotide (PDRN): Studies have found that this ingredient can promote collagen synthesis, inhibit metalloproteinases and elastases, reshape the extracellular matrix, and fight skin aging; it can also reduce melanin production in melanocytes, inhibit tyrosinase activity, and the expression of MITF (microphthalmia-related transcription factor) and TRP-1 (tyrosinase-related protein-1).

[0030] Centella asiatica extract: has antioxidant effects, can inhibit free radical activity, improve skin blood circulation, and regenerate skin cells; can tighten the connection between the epidermis and dermis, make the skin softer, help solve the problem of skin sagging, and make the skin smoother and more elastic; can help promote the formation of collagen in the dermis of the skin, regenerate fibrin, help reduce skin tension lines, and make the skin firm and smooth.

[0031] Purslane Extract: It contains vitamin C, vitamin E, glutathione and beta-carotene, which give it good antioxidant and anti-inflammatory effects. Because it contains vitamin A, it helps support the body's cell division process and is beneficial for regulating skin cell renewal.

[0032] Calendula extract: The main active ingredients are carotenoid compounds, mainly lutein, zeaxanthin, carotene and a large amount of lutein esters, which have anti-inflammatory and antibacterial effects, and have a good inhibitory effect on staphylococci and streptococci; and have a high proliferation effect on epidermal cells and dermal cells, and have a good inhibitory effect on elastase activity.

[0033] The present invention also provides a method for preparing the above salmon facial mask, comprising the following steps:

[0034] (1) adding water to the prescribed amount of aloe vera gel, sodium carrageenan, sodium alginate, konjac gum and glycerin, stirring evenly, heating and stirring in a water bath to obtain a gelatinous liquid;

[0035] (2) adding Centella asiatica extract, Portulaca oleracea extract and Calendula officinalis extract to the colloidal liquid obtained in step (1), stirring evenly, and then adding polydeoxyribonucleotide, hyaluronic acid, acetyl tetrapeptide-15, palmitoyl tetrapeptide-7, acetyl hexapeptide-8, cono peptide, snake venom tripeptide and pentapeptide-3, and continuing to stir to fully mix to obtain a gel solution;

[0036] (3) placing the gel solution obtained in step (2) in a facial mask mold, cooling it to room temperature, and pressing it into a film to obtain the salmon facial mask.

[0037] The temperature of the water bath heating in the above step (1) is 50-80°C.

[0038] Compared with the prior art, the present invention has the following beneficial effects:

[0039] (1) The present invention provides a salmon facial mask, which uses aloe vera gel, sodium carrageenan, sodium alginate and konjac gum as matrix components, and the film-forming properties are significantly improved by controlling the mass ratio of the four components to 2-5:1-3:2-4:1-5; preferably, the mass ratio of aloe vera gel, sodium carrageenan, sodium alginate and konjac gum is controlled to be 4:2:3:4, so that the transparency, spreadability and uniformity of the facial mask are significantly improved.

[0040] (2) The present invention adds natural plant extracts Centella asiatica extract, Portulaca oleracea extract and Calendula officinalis extract to the facial mask. The ingredients are safe and non-irritating. By controlling the mass ratio of the three, not only the anti-inflammatory and repairing effects of the facial mask are improved, but also the facial mask has the effects of moisturizing, anti-oxidation, increasing skin elasticity and repairing.

[0041] (3) The present invention also adds acetyl tetrapeptide-15, palmitoyl tetrapeptide-7, acetyl hexapeptide-8, snake venom tripeptide and pentapeptide-3 to the components, which significantly enhances the anti-inflammatory effect of the mask and also significantly improves the water retention and anti-aging effects of the mask.

[0042] (4) The salmon mask provided by the present invention does not need to be laminated with non-woven fabric and can be quickly dissolved at 60-70°C to become a liquid state. Therefore, the mask has a wider range of application scenarios. When the user is short on time, the mask can be directly dissolved and applied to the skin, which is convenient and saves trouble. DETAILED DESCRIPTION

[0043] The following will be combined with the tables in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0044] Hereinafter, examples and comparative examples of the present invention will be shown to explain the composition of the present invention in more detail, but the present invention is not limited thereto.

[0045] The present invention does not limit the sources of the raw materials used. Unless otherwise specified, the raw materials used in the present invention are common commercial products in the technical field.

[0046] The manufacturers of the components in the following examples are shown in Table 1 below:

[0047] Table 1

[0048]

[0049] Example 1 A salmon mask with anti-inflammatory, anti-aging and repair effects

[0050] The following components are included in parts by weight:

[0051] Phase A: 2 parts of aloe vera gel, 1 part of sodium carrageenan, 2 parts of sodium alginate, 1 part of konjac gum, 3 parts of glycerin and 91 parts of high-purity water;

[0052] Phase B: 5 parts of polydeoxyribonucleotides, 10 parts of Centella asiatica extract, 10 parts of Portulaca oleracea extract, 5 parts of Calendula officinalis extract, 10 parts of hyaluronic acid, 2 parts of acetyl tetrapeptide-15, 1 part of palmitoyl tetrapeptide-7, 1 part of acetyl hexapeptide-8, 3 parts of cono peptide, 1 part of snake venom tripeptide and 2 parts of pentapeptide-3.

[0053] Preparation method: comprising the following steps:

[0054] (1) Add water to the prescribed amount of aloe vera gel, sodium carrageenan, sodium alginate, konjac gum and glycerin, stir evenly, heat and stir in a water bath to obtain a gelatinous liquid;

[0055] (2) adding Centella asiatica extract, Portulaca oleracea extract and Calendula officinalis extract to the colloidal liquid obtained in step (1), stirring evenly, and then adding polydeoxyribonucleotide, hyaluronic acid, acetyl tetrapeptide-15, palmitoyl tetrapeptide-7, acetyl hexapeptide-8, cono peptide, snake venom tripeptide and pentapeptide-3, and continuing to stir to fully mix to obtain a gel solution;

[0056] (3) placing the gel solution obtained in step (2) in a facial mask mold, cooling it to room temperature, and pressing it into a film to obtain the salmon facial mask.

[0057] Example 2 A salmon mask with anti-inflammatory, anti-aging and repair effects

[0058] The components include the following in parts by weight:

[0059] Phase A: 5 parts of aloe vera gel, 3 parts of sodium carrageenan, 4 parts of sodium alginate, 5 parts of konjac gum, 5 parts of glycerin and 78 parts of high-purity water;

[0060] Phase B: 10 parts of polydeoxyribonucleotides, 25 parts of Centella asiatica extract, 25 parts of Portulaca oleracea extract, 15 parts of Calendula officinalis extract, 20 parts of hyaluronic acid, 5 parts of acetyl tetrapeptide-15, 3 parts of palmitoyl tetrapeptide-7, 3 parts of acetyl hexapeptide-8, 8 parts of cono peptide, 3 parts of snake venom tripeptide and 5 parts of pentapeptide-3.

[0061] Preparation method: comprising the following steps:

[0062] (1) Add water to the prescribed amount of aloe vera gel, sodium carrageenan, sodium alginate, konjac gum and glycerin, stir evenly, heat and stir in a water bath to obtain a gelatinous liquid;

[0063] (2) adding Centella asiatica extract, Portulaca oleracea extract and Calendula officinalis extract to the colloidal liquid obtained in step (1), stirring evenly, and then adding polydeoxyribonucleotide, hyaluronic acid, acetyl tetrapeptide-15, palmitoyl tetrapeptide-7, acetyl hexapeptide-8, cono peptide, snake venom tripeptide and pentapeptide-3, and continuing to stir to fully mix to obtain a gel solution;

[0064] (3) placing the gel solution obtained in step (2) in a facial mask mold, cooling it to room temperature, and pressing it into a film to obtain the salmon facial mask.

[0065] Example 3 A salmon mask with anti-inflammatory, anti-aging and repair effects

[0066] The components include the following in parts by weight:

[0067] Phase A: 3 parts of aloe vera gel, 2.5 parts of sodium carrageenan, 2.5 parts of sodium alginate, 3 parts of konjac gum, 4 parts of glycerin and 85 parts of high-purity water;

[0068] Phase B: 6 parts of polydeoxyribonucleotides, 15 parts of Centella asiatica extract, 15 parts of Portulaca oleracea extract, 12 parts of Calendula officinalis extract, 18 parts of hyaluronic acid, 3 parts of acetyl tetrapeptide-15, 2.5 parts of palmitoyl tetrapeptide-7, 2.5 parts of acetyl hexapeptide-8, 6 parts of cono peptide, 1.5 parts of snake venom tripeptide and 3 parts of pentapeptide-3.

[0069] Preparation method: comprising the following steps:

[0070] (1) Add water to the prescribed amount of aloe vera gel, sodium carrageenan, sodium alginate, konjac gum and glycerin, stir evenly, heat and stir in a water bath to obtain a gelatinous liquid;

[0071] (2) adding Centella asiatica extract, Portulaca oleracea extract and Calendula officinalis extract to the colloidal liquid obtained in step (1), stirring evenly, and then adding polydeoxyribonucleotide, hyaluronic acid, acetyl tetrapeptide-15, palmitoyl tetrapeptide-7, acetyl hexapeptide-8, cono peptide, snake venom tripeptide and pentapeptide-3, and continuing to stir to fully mix to obtain a gel solution;

[0072] (3) placing the gel solution obtained in step (2) in a facial mask mold, cooling it to room temperature, and pressing it into a film to obtain the salmon facial mask.

[0073] Example 4 A salmon mask with anti-inflammatory, anti-aging and repair effects

[0074] The following components are included in parts by weight:

[0075] Phase A: 4 parts of aloe vera gel, 2 parts of sodium carrageenan, 3 parts of sodium alginate, 4 parts of konjac gum, 5 parts of glycerin and 82 parts of high-purity water;

[0076] Phase B: 8 parts of polydeoxyribonucleotides, 18 parts of Centella asiatica extract, 18 parts of Portulaca oleracea extract, 10 parts of Calendula officinalis extract, 15 parts of hyaluronic acid, 4 parts of acetyl tetrapeptide-15, 2 parts of palmitoyl tetrapeptide-7, 2 parts of acetyl hexapeptide-8, 5 parts of cono peptide, 2 parts of snake venom tripeptide and 4 parts of pentapeptide-3.

[0077] Preparation method: comprising the following steps:

[0078] (1) Add water to the prescribed amount of aloe vera gel, sodium carrageenan, sodium alginate, konjac gum and glycerin, stir evenly, heat and stir in a water bath to obtain a gelatinous liquid;

[0079] (2) adding Centella asiatica extract, Portulaca oleracea extract and Calendula officinalis extract to the colloidal liquid obtained in step (1), stirring evenly, and then adding polydeoxyribonucleotide, hyaluronic acid, acetyl tetrapeptide-15, palmitoyl tetrapeptide-7, acetyl hexapeptide-8, cono peptide, snake venom tripeptide and pentapeptide-3, and continuing to stir to fully mix to obtain a gel solution;

[0080] (3) placing the gel solution obtained in step (2) in a facial mask mold, cooling it to room temperature, and pressing it into a film to obtain the salmon facial mask.

[0081] Comparative Example 1

[0082] The difference from Example 4 is that konjac gum, namely 6 parts of sodium carrageenan, is omitted from the ingredients of phase A, and the other components and preparation method are the same as those of Example 4.

[0083] Comparative Example 2

[0084] The difference from Example 4 is that the Centella asiatica extract is replaced by the Taraxacum officinale extract, and the other components and preparation method are the same as those of Example 4.

[0085] Comparative Example 3

[0086] The difference from Example 4 is that acetyl tetrapeptide-15 and acetyl hexapeptide-8 are replaced by palmitoyl tetrapeptide-7, that is:

[0087] Phase B: 8 parts of polydeoxyribonucleotides, 18 parts of Centella asiatica extract, 18 parts of Portulaca oleracea extract, 10 parts of Calendula officinalis extract, 15 parts of hyaluronic acid, 8 parts of palmitoyl tetrapeptide-7, 5 parts of cono peptide, 2 parts of snake venom tripeptide and 4 parts of pentapeptide-3.

[0088] The other components and preparation methods are the same as those in Example 4.

[0089] Comparative Example 4

[0090] The difference from Example 4 is that the mass ratio of Centella asiatica extract, Portulaca oleracea extract and Calendula officinalis extract is 1:1:3, i.e. 9.2 parts of Centella asiatica extract, 9.2 parts of Portulaca oleracea extract and 27.6 parts of Calendula officinalis extract, and the other components and preparation method are the same as those in Example 4.

[0091] Comparative Example 5

[0092] The difference from Example 4 is that the mass ratio of acetyl tetrapeptide-15, palmitoyl tetrapeptide-7, acetyl hexapeptide-8, snake venom-like tripeptide and pentapeptide-3 is 1:3:2:3:1, that is, 1.4 parts of acetyl tetrapeptide-15, 5.2 parts of palmitoyl tetrapeptide-7, 2.8 parts of acetyl hexapeptide-8, 5.2 parts of snake venom-like tripeptide and 1.4 parts of pentapeptide-3, and the other components and preparation method are the same as those in Example 4.

[0093] Effect experiment:

[0094] 1. Mask appearance performance

[0095] The smoothness and color of the gel mask were used as evaluation criteria. Evaluation criteria:

[0096] 2-4 points: uneven surface, no color; 4.1-6 points: flat surface, average color; 6.1-8 points: smooth surface, good color; 8.1-10 points: smooth surface, good color. Ten samples were made for each group, and the scores were calculated to obtain the average value.

[0097] The scoring results are shown in Table 2 below.

[0098] Table 2

[0099]

[0100] According to the scoring results in Table 2 above, the gel masks prepared in Examples 1-4 of the present invention have better apparent performance, and the smoothness and color scores of the masks are all above 9.0, while the smoothness and color of the gel mask obtained by omitting konjac gum in Comparative Example 1 are significantly lower than those in the embodiment.

[0101] 2. In vitro antibacterial test

[0102] In vitro inhibitory activity against Staphylococcus aureus and Candida albicans.

[0103] Experimental strain preparation:

[0104] The gel masks prepared in Examples 1-4 and Comparative Examples 2-5 were dissolved in water at 65°C, and then diluted with a diluent to a final concentration of 5% to obtain a test sample. Diluent: 0.03 mol / L phosphate buffer (PBS) (pH 7.2-7.4), culture medium: nutrient agar medium was used for the culture of Staphylococcus aureus, and Sabouraud agar medium was used for the culture of Candida albicans.

[0105] Experimental methods:

[0106] Take the 24h fresh slant culture of the test bacteria, wash it with PBS, and dilute it with PBS to about 5.0×10 5 CFU / mL-4.5×10 6 CFU / mL bacterial suspension is ready for use. Take a sterile test tube, add 5.0mL of the test sample first, place it in a water bath at 20°C±1°C for 5 minutes, add 0.1mL of the test bacterial suspension, mix quickly and start the time immediately. Wait for the test bacteria to interact with the test sample for 5 minutes, then take 1.0mL of the test bacteria and test sample mixture to inoculate 2 plates respectively, and pour the culture medium. When the amount of bacteria cannot be counted, make a 10-fold serial dilution with PBS, select the appropriate dilution and take 1.0mL to inoculate 2 plates respectively, and count the live bacteria culture. At the same time, use PBS instead of the sample to conduct a parallel test as a positive control. The number of colonies recovered in the positive control is 1.0×10 4 CFU / mL-9.0×10 4 CFU / mL. Take the same batch of PBS and culture medium as negative control. All test samples and control samples were cultured at 36°C±1°C, and the final results were observed after 48h of bacterial propagule culture; the final results of Candida albicans were observed after 72h of culture. The test was repeated 3 times and the inhibition rate was calculated.

[0107] Calculation of antibacterial rate: X=

[0108] Where: X-inhibition rate, %;

[0109] A 0 -The amount of bacteria recovered in the positive control group, in CFU / mL;

[0110] A 1 -The amount of bacteria recovered in the test group, the unit is CFU / mL.

[0111] The test results are shown in Table 3 below.

[0112] Table 3

[0113]

[0114] According to the test results in Table 3 above, the gel masks prepared in Examples 1-4 of the present invention have better antibacterial effects, with the antibacterial rates against Staphylococcus aureus reaching more than 92%, and the antibacterial rates against Candida albicans reaching more than 85%; while changing the types or ratios of the components in the comparative example will significantly reduce the inhibition rates of the gel masks against Staphylococcus aureus and Candida albicans.

[0115] 3. Elastase inhibition performance

[0116] Experimental method: The gel mask prepared in Examples 1-4 and Comparative Examples 2-5 was dissolved in water at 65°C, and then diluted to a final concentration of 10% to obtain a test sample; the following were added to a 96-well plate: 25 μl, 0.1M HEPES buffer, 50 μl sample and 25 μl elastase were added to the experimental group; 75 μl HEPES buffer was added to the blank group, and 25 μl elastase and 50 μl HEPES buffer were added to the negative control group; 25 μl elastase, 25 μl, 0.1M HEPES buffer and 50 μl water were added to the solvent control group. After the 96-well plate was incubated at room temperature for 20 minutes, the absorbance A was measured by a multifunctional fluorescent microplate reader, and the detection wavelength was 405 nm. Triplicate. The elastase inhibition rate was calculated.

[0117] Elastase inhibition rate (%) = (Ac–At / Ac) × 100%.

[0118] Here, Ac is the absorbance value of the solvent control group, and At is the absorbance value of the positive control or experimental group. The experimental results are averaged, and the test results are shown in Table 4 below.

[0119] Table 4

[0120]

[0121] According to the test results in Table 4 above, the gel masks prepared in Examples 1-4 of the present invention have better elastase inhibition rate, and the inhibition rate against elastase can reach more than 44%; while changing the type or ratio of each component in the comparative example will significantly reduce the inhibition rate of the gel mask against elastase.

[0122] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that they can still modify or replace the technical solution of the present invention with equivalents, and these modifications or equivalent replacements cannot cause the modified technical solution to deviate from the spirit and scope of the technical solution of the present invention.

Claims

1. A salmon facial mask with anti-inflammatory, anti-aging and repairing effects, characterized in that: The following components are included in parts by weight: Phase A: 2-5 parts of aloe vera gel, 1-3 parts of sodium carrageenan, 2-4 parts of sodium alginate, 1-5 parts of konjac gum, 3-5 parts of glycerin and 75-95 parts of high-purity water; Phase B: 5-10 parts of polydeoxyribonucleotides, 10-25 parts of Centella asiatica extract, 10-25 parts of Portulaca oleracea extract, 5-15 parts of Calendula officinalis extract, 10-20 parts of hyaluronic acid, 2-5 parts of acetyl tetrapeptide-15, 1-3 parts of palmitoyl tetrapeptide-7, 1-3 parts of acetyl hexapeptide-8, 3-8 parts of cono peptide, 1-3 parts of snake venom tripeptide and 2-5 parts of pentapeptide-3.

2. The salmon facial mask according to claim 1, characterized in that: The following components are included in parts by weight: Phase A: 3-4 parts of aloe vera gel, 1.5-2.5 parts of sodium carrageenan, 2.5-3.5 parts of sodium alginate, 2-4 parts of konjac gum, 4-5 parts of glycerin and 78-92 parts of high-purity water; Phase B: 6-8 parts of polydeoxyribonucleotides, 15-20 parts of Centella asiatica extract, 15-20 parts of Portulaca oleracea extract, 8-12 parts of Calendula officinalis extract, 12-18 parts of hyaluronic acid, 3-4 parts of acetyl tetrapeptide-15, 1.5-2.5 parts of palmitoyl tetrapeptide-7, 1.5-2.5 parts of acetyl hexapeptide-8, 4-6 parts of cono peptide, 1.5-2.5 parts of snake venom tripeptide and 3-4 parts of pentapeptide-3.

3. The salmon facial mask according to claim 1, characterized in that: The mass ratio of the aloe vera gel, sodium carrageenan, sodium alginate and konjac gum is 4:2:3:

4.

4. The salmon facial mask according to claim 1, characterized in that: The mass ratio of the Centella asiatica extract, the Portulaca oleracea extract and the Calendula officinalis extract is 1.8:1.8:

1.

5. The salmon facial mask according to claim 1, characterized in that: The mass ratio of acetyl tetrapeptide-15, palmitoyl tetrapeptide-7, acetyl hexapeptide-8, snake venom tripeptide and pentapeptide-3 is 1-5:1:1:1:1-5.

6. The salmon facial mask according to claim 5, characterized in that: The mass ratio of acetyl tetrapeptide-15, palmitoyl tetrapeptide-7, acetyl hexapeptide-8, snake venom-like tripeptide and pentapeptide-3 is 2:1:1:1:

2.

7. The salmon facial mask according to any one of claims 1 to 6, characterized in that: The salmon facial mask comprises the following components in parts by weight: Phase A: 4 parts of aloe vera gel, 2 parts of sodium carrageenan, 3 parts of sodium alginate, 4 parts of konjac gum, 5 parts of glycerin and 82 parts of high-purity water; Phase B: 8 parts of polydeoxyribonucleotides, 18 parts of Centella asiatica extract, 18 parts of Portulaca oleracea extract, 10 parts of Calendula officinalis extract, 15 parts of hyaluronic acid, 4 parts of acetyl tetrapeptide-15, 2 parts of palmitoyl tetrapeptide-7, 2 parts of acetyl hexapeptide-8, 5 parts of cono peptide, 2 parts of snake venom tripeptide and 4 parts of pentapeptide-3.

8. The method for preparing the salmon facial mask according to any one of claims 1 to 6, characterized in that: The steps include: (1) adding water to the prescribed amount of aloe vera gel, sodium carrageenan, sodium alginate, konjac gum and glycerin, stirring evenly, heating and stirring in a water bath to obtain a gelatinous liquid; (2) adding Centella asiatica extract, Portulaca oleracea extract and Calendula officinalis extract to the colloidal liquid obtained in step (1), stirring evenly, and then adding polydeoxyribonucleotide, hyaluronic acid, acetyl tetrapeptide-15, palmitoyl tetrapeptide-7, acetyl hexapeptide-8, cono peptide, snake venom tripeptide and pentapeptide-3, and continuing to stir to fully mix to obtain a gel solution; (3) placing the gel solution obtained in step (2) in a facial mask mold, cooling it to room temperature, and pressing it into a film to obtain the salmon facial mask.

9. The preparation method according to claim 8, characterized in that: The temperature of the water bath heating in step (1) is 50-80°C.

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