Traditional Chinese medicine probiotic composition for improving type 2 diabetes mellitus as well as preparation method and application of traditional Chinese medicine probiotic composition

Abstract

The invention belongs to the technical field of pharmaceutical compositions, and discloses a traditional Chinese medicine probiotic composition for improving type 2 diabetes and a preparation method and application thereof. The composition is obtained by fermenting a traditional Chinese medicine composition with a compound probiotic liquid, wherein traditional Chinese medicine components in the traditional Chinese medicine composition liquid comprise, by weight, 5-10 parts of radix puerariae, 2-3 parts of honey-fried licorice roots, 3-4 parts of radix scutellariae and 3-4 parts of coptis chinensis, and the composite probiotic liquid is obtained by mixing a lactobacillus plantarum liquid and a pediococcus pentosaceus liquid according to the volume ratio of (0.3-3): 1. The traditional Chinese medicine probiotic composition provided by the invention contains probiotics (lactobacillus and the like), and the intervention of the radix puerariae, the rhizoma coptidis, the radix scutellariae and the liquorice can promote the growth of intestinal probiotics, regulate the alteration of intestinal flora and improve the clinical treatment effect of patients with type 2 diabetes mellitus.

Description

Technical Field

[0001] The present invention relates to the technical field of pharmaceutical compositions, and in particular to a traditional Chinese medicine probiotic composition for improving type 2 diabetes, and a preparation method and application thereof. Background Art

[0002] Among the treatments for type 2 diabetes, combined therapy has become one of the important means. Traditional Chinese medicine and probiotics are used as a new direction to assist in improving type 2 diabetes because they can reshape the intestinal flora. Chinese patent CN 115990211 A discloses a Chinese medicine probiotic composition for lowering blood pressure and a preparation method thereof, which is composed of probiotic powder (Lactobacillus, Bifidobacterium, etc.), chrysanthemum, coptis root, and salvia miltiorrhiza. Chinese patent CN 118453787 A discloses a Chinese medicine probiotic composition and a preparation method thereof, wherein the Chinese medicine mixture includes Phellodendron, Smilax glabra, Lysimachia chinensis, etc.; the composite probiotic powder includes Lactobacillus acidophilus, etc., and the modified composition has the effect of reducing uric acid and treating gout. At present, these Chinese medicine mixtures and probiotic powders are mostly related to hypertension and hyperuricemia, and rarely involve improving type 2 diabetes. In addition, the above-mentioned compound Chinese medicine is just a simple patchwork of many single-flavor medicines, without a certain amount of clinical verification, and without the support of traditional Chinese medicine theory, and its clinical efficacy remains to be evaluated.

[0003] In view of this, the present invention is proposed. Summary of the invention

[0004] To solve the problems in the background technology, the present invention provides a Chinese medicinal probiotic composition for improving type 2 diabetes, and a preparation method and application thereof.

[0005] To achieve the above object, the first technical solution adopted by the present invention is: A Chinese medicinal probiotic composition for improving type 2 diabetes is obtained by fermenting a Chinese medicinal composition liquid with a composite probiotic liquid; wherein, by weight, the Chinese medicinal ingredients in the Chinese medicinal composition are 5-10 parts of Pueraria root, 2-3 parts of Glycyrrhiza uralensis, 3-4 parts of Scutellaria baicalensis, and 3-4 parts of Coptis chinensis; and the composite probiotic liquid is obtained by mixing a Lactobacillus plantarum liquid and a Pediococcus pentosaceus liquid in a volume ratio of (0.3-3):1.

[0006] Preferably, the Chinese medicinal ingredients in the Chinese medicinal composition liquid are 5 parts of Pueraria root, 2 parts of Radix Glycyrrhizae Preparata, 3 parts of Scutellaria baicalensis, and 3 parts of Coptis chinensis.

[0007] Preferably, the volume ratio of the Chinese medicine composition liquid to the composite probiotic liquid is 50:1, and the viable bacterial counts of the Lactobacillus plantarum liquid and the Pediococcus pentosaceus liquid are 1.0×10 8 CFU / mL.

[0008] The second technical solution provided by the present invention is: A method for preparing a Chinese medicinal probiotic composition for improving type 2 diabetes, comprising: Boiling the Chinese medicine with water and then vacuum filtering to obtain a Chinese medicine composition liquid; The composite probiotic bacterial liquid is added to the Chinese medicine composition liquid for cultivation and allowed to stand.

[0009] Preferably, the Chinese medicine is crushed and then added to water and boiled.

[0010] Preferably, the traditional Chinese medicine is crushed and dissolved, the modified cellulose is added and mixed evenly, and then the mixture is filtered and boiled.

[0011] Preferably, the composite probiotics solution is added to the Chinese medicine composition solution cooled to 30° C. and cultured for 36-48 hours, then cooled to 28° C. and cultured for another 48-56 hours.

[0012] Preferably, the mixture is allowed to stand at 4°C for 4-6 hours.

[0013] The third technical solution adopted by the present invention is: Application of a traditional Chinese medicine probiotic composition for improving type 2 diabetes in the preparation of a drug for improving type 2 diabetes.

[0014] Compared with the prior art, the present invention has the following beneficial effects: The Chinese medicinal probiotic composition provided by the present invention contains probiotics (such as lactobacillus). The intervention of four Chinese medicinal herbs, namely, kudzu root, coptis root, scutellaria baicalensis and liquorice, can promote the growth of intestinal probiotics, regulate intestinal flora imbalance, and improve the clinical treatment effect of patients with type 2 diabetes. DETAILED DESCRIPTION

[0015] The technical solution of the present invention will be clearly and completely described below in conjunction with the embodiments. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0016] The first embodiment of the present invention provides a Chinese medicinal probiotic composition for improving type 2 diabetes, which is obtained by fermenting a Chinese medicinal composition liquid with a composite probiotic liquid; wherein, by weight, the Chinese medicinal ingredients in the Chinese medicinal composition are 5-10 parts of Pueraria root, 2-3 parts of Glycyrrhiza uralensis, 3-4 parts of Scutellaria baicalensis, and 3-4 parts of Coptis chinensis, and the composite probiotic liquid is obtained by mixing a Lactobacillus plantarum liquid and a Pediococcus pentosaceus liquid in a volume ratio of (0.3-3):1.

[0017] In the embodiment of the present invention, the Chinese medicine probiotic composition obtained by fermenting the Chinese medicine composition liquid with Lactobacillus plantarum and Pediococcus pentosaceus significantly improves type 2 diabetes.

[0018] In some preferred embodiments, the Chinese medicinal ingredients in the Chinese medicinal composition liquid are 5 parts of Pueraria root, 2 parts of Radix Glycyrrhizae Preparata, 3 parts of Scutellaria baicalensis, and 3 parts of Coptis chinensis.

[0019] In some preferred embodiments, the volume ratio of the Chinese medicine composition liquid to the composite probiotic liquid is 50:1, and the viable bacterial counts of the Lactobacillus plantarum liquid and the Pediococcus pentosaceus liquid are 1.0×10 8 CFU / mL.

[0020] The second embodiment of the present invention provides a method for preparing a Chinese medicine probiotic composition for improving type 2 diabetes, comprising: boiling Chinese medicine with water and then vacuum filtering to obtain a Chinese medicine composition liquid; adding a composite probiotic liquid to the Chinese medicine composition liquid for culturing and standing.

[0021] The volume ratio of the Chinese medicine mass to water in the Chinese medicine composition is (13-21): 100 g / L.

[0022] In order to improve the efficacy and drug quality of the Chinese herbal probiotic composition, modified cellulose can be added during the preparation process to improve the solubility, taste and stability of the Chinese herbal probiotic composition.

[0023] Specifically, modified cellulose can be added to the Chinese medicine composition liquid; the specific amount of modified cellulose can be selected by those skilled in the art according to actual needs.

[0024] Understandably, in order to fully release the effective ingredients in the traditional Chinese medicine, the traditional Chinese medicine can be crushed and then added to water for boiling.

[0025] In some preferred embodiments, the traditional Chinese medicine is crushed and dissolved, the modified cellulose is added and mixed evenly, and the mixture is filtered and then boiled.

[0026] In some preferred embodiments, the composite probiotic solution is added to the Chinese medicine composition solution cooled to 30° C. and cultured for 36-48 hours, then cooled to 28° C. and cultured for another 48-56 hours.

[0027] In some preferred embodiments, the mixture is allowed to stand at 4°C for 4-6 hours.

[0028] The third embodiment of the present invention provides the use of a Chinese medicinal probiotic composition for improving type 2 diabetes in the preparation of a drug for improving type 2 diabetes.

[0029] In order to make the technical solution of the present invention clearer, the preparation method and effect of the Chinese medicine probiotic composition are described below through multiple specific embodiments.

[0030] Example 1 Weigh 9g of Pueraria root, 2g of licorice root, 3g of Scutellaria baicalensis and 3g of Coptis chinensis, grind and mix them, and sieve them through 55 mesh to obtain Chinese medicine powder; add 100L of distilled water to dissolve the Chinese medicine powder, add 13g of modified cellulose, filter, then heat to 100℃ and boil for 45min, and then vacuum and filter to obtain the liquid that is the Chinese medicine composition liquid; place the Chinese medicine composition liquid in a fermentation tank, cool to 30℃, and add 2% of the volume of the Chinese medicine composition liquid activated composite probiotics liquid (the volume ratio of Lactobacillus plantarum and Pediococcus pentosaceus is 3:1, and the number of viable bacteria is 1.0×10 8 CFU / mL) for 36 h, then cooled to 28°C and cultured for another 48 h to obtain the Chinese medicine probiotic fermentation liquid. Standing: The Chinese medicine probiotic fermentation liquid was placed at 4°C for 4 h to obtain the Chinese medicine probiotic composition for improving type 2 diabetes.

[0031] Example 2 Weigh 9g of Pueraria root, 2g of licorice root, 3g of Scutellaria baicalensis and 3g of Coptis chinensis, grind and mix them, and sieve through 55 mesh to obtain Chinese medicine powder; add 100L of distilled water to dissolve the Chinese medicine powder, add 15g of modified cellulose, filter, then heat to 100℃ and boil for 50min, and then vacuum and filter to obtain the liquid of Chinese medicine composition liquid; place the Chinese medicine composition liquid in a fermentation tank, cool to 30℃, and add 2% of the volume of the Chinese medicine composition liquid activated composite probiotics liquid (the volume ratio of Lactobacillus plantarum and Pediococcus pentosaceus is 2:2, and the number of viable bacteria is 1.0×10 8 CFU / mL) for 36 h, then cooled to 30°C and cultured for another 48 h to obtain the Chinese medicine probiotic fermentation liquid. Standing: The Chinese medicine probiotic fermentation liquid was placed at 4°C for 6 h to obtain the Chinese medicine probiotic composition for improving type 2 diabetes.

[0032] Example 3 Weigh 9g of Pueraria root, 2g of licorice root, 3g of Scutellaria baicalensis and 3g of Coptis chinensis, grind and mix them, and sieve them through 55 mesh to obtain Chinese medicine powder; add 100L of distilled water to dissolve the Chinese medicine powder, add 13g of modified cellulose, filter, then heat to 100℃ and boil for 50 min, and then vacuum and filter to obtain the liquid that is the Chinese medicine composition liquid; place the Chinese medicine composition liquid in a fermentation tank, cool to 30℃, and add 2% of the volume of the Chinese medicine composition liquid activated composite probiotics liquid (the volume ratio of Lactobacillus plantarum and Pediococcus pentosaceus is 1:3, and the number of viable bacteria is 1.0×10 8 CFU / mL) for 48 h, then cooled to 30°C and cultured for another 56 h to obtain the Chinese medicine probiotic fermentation liquid. Standing: The Chinese medicine probiotic fermentation liquid was placed at 4°C for 6 h to obtain the Chinese medicine probiotic composition for improving type 2 diabetes.

[0033] Experimental example Experimental animals SPF grade Wistar male rats, 6 weeks old (160-200 g), were provided by Chengdu Dashuo Animal Experiment Company.

[0034] Reagents: Streptozotocin STZ (Sigma, USA); Chloral hydrate (Shanghai Xinyu Biotechnology Co., Ltd.); Metformin (Shanghai Bristol-Myers Squibb Pharmaceuticals Co., Ltd.); Basic feed (Huaxi Animal House, Sichuan University); High-fat feed (Chengdu Dashuo Animal Experiment Company) 1. Construction of T2DM rat model For the establishment of T2DM rat model, this study adopted the intraperitoneal injection of 30 mg / kg STZ according to the body weight of rats after one week of high-fat feeding. Fasting blood glucose was ≥11.0 mmol / L, which was considered as successful T2DM rat modeling, with 6 rats in each group. Then, the T2DM rats were randomly divided into 7 groups (n = 3), namely, NC normal group (NC group), T2DM model group (M group), Example 1 (Chinese herbal probiotic composition 1), Example 2 (Chinese herbal probiotic composition 2), Example 3 (Chinese herbal probiotic composition 3), probiotic group (only using the probiotics in Example 1), Chinese herbal composition group (only using the probiotics in Example 1), and metformin treatment group (MET group). The NC group and the M group were gavaged with 2 mL of 0.85% normal saline, and the MET group was gavaged with 150 mg / kg of metformin every day.

[0035] 2. Effects of Chinese herbal probiotic composition on fasting blood glucose and postprandial blood glucose in rats During the 6-week period of intragastric administration of the samples in each group of 1, the blood glucose was tested every weekend by taking blood from the rat's tail and using a rapid blood glucose meter. The blood glucose value of each rat was repeated 3 times each time, and the average value was taken as the final blood glucose value. The rats were fasted overnight but not watered for 12 hours to measure their fasting blood glucose (FBG), and their postprandial blood glucose (PBG) was measured 2 hours after eating, without fasting or watering. Random blood glucose (RBG) was measured at a unified time point (Sunday 14:00). The results are shown in Table 1.

[0036] Table 1 Effects of the composition of the invention on fasting blood glucose and postprandial blood glucose in rats .

[0037] In summary, by comparing the positive control group, the blank group, the present invention group and the metformin group, the following conclusions can be drawn: The Chinese herbal probiotic composition has a certain effect of lowering fasting blood sugar and postprandial blood sugar.

[0038] The proportion of plant lactobacillus was higher and the culture time was longer, and the drug complex group was able to better improve the symptoms of type 2 diabetes.

[0039] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the above embodiments, it should be understood by those skilled in the art that the technical solutions described in the above embodiments may still be modified, or some or all of the technical features thereof may be replaced by equivalents. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A Chinese medicinal probiotic composition for improving type 2 diabetes, characterized in that: The method is obtained by fermenting a traditional Chinese medicine composition liquid with a composite probiotic liquid; wherein, by weight, the traditional Chinese medicine components in the traditional Chinese medicine composition liquid are 5-10 parts of Pueraria root, 2-3 parts of Glycyrrhiza uralensis, 3-4 parts of Scutellaria baicalensis, and 3-4 parts of Coptis chinensis; and the composite probiotic liquid is obtained by mixing a Lactobacillus plantarum liquid and a Pediococcus pentosaceus liquid in a volume ratio of (0.3-3):

1.

2. The Chinese medicinal probiotic composition according to claim 1, characterized in that: The Chinese medicinal ingredients in the Chinese medicinal composition liquid are 5 parts of Pueraria root, 2 parts of Radix Glycyrrhizae Preparata, 3 parts of Scutellariae baicalensis and 3 parts of Coptis chinensis.

3. The Chinese medicinal probiotic composition according to claim 1, characterized in that: The volume ratio of the Chinese medicine composition liquid to the composite probiotic liquid was 50:1, and the viable bacterial counts of the Lactobacillus plantarum liquid and the Pediococcus pentosaceus liquid were both 1.0×10 8 CFU / mL.

4. The method for preparing the Chinese medicinal probiotic composition for improving type 2 diabetes as claimed in any one of claims 1 to 3, characterized in that: Include: Boiling the Chinese medicine with water and then vacuum filtering to obtain a Chinese medicine composition liquid; The composite probiotic bacterial liquid is added to the Chinese medicine composition liquid for cultivation and standing.

5. The preparation method according to claim 4, characterized in that: Crush the Chinese medicine and add water to boil.

6. The preparation method according to claim 5, characterized in that: Crush and dissolve the traditional Chinese medicine, add modified cellulose and mix evenly, filter and boil.

7. The preparation method according to claim 4, characterized in that: The composite probiotics solution is added to the Chinese medicine composition solution cooled to 30° C. and cultured for 36-48 hours, then cooled to 28° C. and cultured for another 48-56 hours.

8. The preparation method according to claim 4, characterized in that: Incubate at 4°C for 4-6 hours.

9. Use of the Chinese medicinal probiotic composition for improving type 2 diabetes as claimed in any one of claims 1 to 3 in the preparation of a drug for improving type 2 diabetes.