Universal automatic nucleic acid extraction module based on rotating structure

Abstract

The invention relates to the technical field of nucleic acid detection, and discloses a universal automatic nucleic acid extraction module based on a rotating structure, which comprises a liquid storage layer and a runner layer which are arranged up and down, the nucleic acid extraction module is internally provided with a lysate chamber, a binding liquid chamber, a magnetic bead chamber, a pre-cleaning liquid chamber, a post-cleaning liquid chamber, an eluent chamber, a sample treatment chamber, a waste liquid chamber and a sample loading chamber; a valve core is arranged in the sample treatment cavity, a sample treatment cavity piston is arranged in a core cavity of the valve core, and a liquid transfer opening communicated with the core cavity is formed in the side wall of the core cavity; the top surface of the flow channel layer is respectively provided with flow channels communicated with the sample treatment chamber corresponding to the lysis solution chamber, the combination solution chamber, the magnetic bead chamber, the front cleaning solution chamber, the rear cleaning solution chamber, the eluent chamber, the waste liquid chamber and the sample loading chamber; the valve element rotates around the axis of the valve element and can communicate with different flow channels. The liquid flow channel is convenient to switch, the structure is simpler, the assembly is simpler, the cost is lower, the size is smaller, and the weight is lighter.

Description

Technical Field

[0001] The present invention relates to the technical field of nucleic acid detection, and in particular to a universal automatic nucleic acid extraction module based on a rotating structure. Background Art

[0002] Nucleic acid testing occupies an extremely important position in pathogen detection due to its high accuracy, sensitivity and specificity, and it currently still exists as the gold standard for clinical diagnosis.

[0003] Nucleic acid extraction methods include cell lysis, centrifugal column, magnetic bead method, etc. The magnetic bead method is to improve and modify the surface of magnetic nanoparticles so that they can reversibly bind and release nucleic acids under certain conditions. The magnetic bead method is simple to operate, short in time, and can be automated and high-throughput, and the nucleic acids obtained are of high purity and high concentration.

[0004] Manually preparing reagents in the nucleic acid extraction process can no longer meet the market's demand for high throughput. Traditional nucleic acid extraction devices often require multiple valves (especially microvalves) to control liquid flow, and highly integrated valve systems may make the overall structure of the device more complex and increase costs. In order to accommodate numerous microvalves and their control circuits, the size and weight of the device are often difficult to reduce, limiting its deployment and mobility in laboratory environments with limited space.

[0005] Therefore, there is an urgent need to improve the traditional nucleic acid extraction device to overcome the above-mentioned defects. Summary of the invention

[0006] The object of the present invention is to provide a universal automatic nucleic acid extraction module based on a rotary structure with simple liquid flow control, so as to reduce the setting of valves and reduce the volume and weight of the nucleic acid extraction module.

[0007] In order to solve the above technical problems, the technical solution of the present invention is as follows:

[0008] A universal automatic nucleic acid extraction module based on a rotating structure, the nucleic acid extraction module comprising a liquid storage layer and a flow channel layer arranged in sequence from top to bottom;

[0009] The nucleic acid extraction module is provided with a lysis liquid chamber, a binding liquid chamber, a magnetic bead chamber, a front cleaning liquid chamber, a rear cleaning liquid chamber, an elution liquid chamber, a sample processing chamber, a waste liquid chamber, and a sample loading chamber;

[0010] The sample processing chamber extends upward through the top surface of the liquid storage layer and extends downward into the flow channel layer. A valve core is installed in the sample processing chamber. A sample processing chamber piston is installed in the core cavity of the valve core. A liquid transfer port communicating with the core cavity is opened on the side wall of the core cavity.

[0011] The top surface of the flow channel layer is provided with flow channels connected to the sample processing chamber, corresponding to the lysate chamber, the binding liquid chamber, the magnetic bead chamber, the front cleaning liquid chamber, the rear cleaning liquid chamber, the elution liquid chamber, the waste liquid chamber, and the sample loading chamber; wherein,

[0012] The valve core can rotate around its own axis to connect the liquid transfer port to different flow channels.

[0013] Furthermore, a vortex-type flow channel is formed on the inner bottom wall of the core cavity of the valve core.

[0014] Furthermore, the magnetic bead chamber is located between the binding liquid chamber and the sample processing chamber, and the flow channel corresponding to the binding liquid chamber extends through the flow channel corresponding to the magnetic bead chamber.

[0015] Furthermore, the flow channels corresponding to the lysis liquid chamber, the binding liquid chamber, the front cleaning liquid chamber, the rear cleaning liquid chamber, and the elution liquid chamber extend radially from one end of a main channel, and the other end of the main channel is connected to the sample processing chamber.

[0016] Furthermore, the lysis liquid chamber, the binding liquid chamber, the magnetic bead chamber, the front cleaning liquid chamber, the rear cleaning liquid chamber, and the elution liquid chamber respectively extend upward through the top surface of the liquid storage layer, and the nucleic acid extraction module also includes a flexible membrane layer arranged on the bottom wall of the liquid storage layer, and the flexible membrane layer is used to seal the bottom ends of the lysis liquid chamber, the binding liquid chamber, the magnetic bead chamber, the front cleaning liquid chamber, the rear cleaning liquid chamber, and the elution liquid chamber located in the liquid storage layer.

[0017] Furthermore, the lysis liquid chamber, the binding liquid chamber, the magnetic bead chamber, the front cleaning liquid chamber, the rear cleaning liquid chamber, and the elution liquid chamber are each equipped with a rubber plug, and each bottom wall is provided with a thorn-like structure, and the thorn-like structure is used to pierce the corresponding part on the flexible membrane layer.

[0018] Furthermore, a pressure buffer chamber is provided in the nucleic acid extraction module, and the pressure buffer chamber extends upward through the top surface of the liquid storage layer and extends downward through the bottom surface of the flow channel layer. A rubber plug is provided in the pressure buffer chamber, and the rubber plug can float up and down in the pressure buffer chamber.

[0019] Furthermore, the nucleic acid extraction module also includes a gasket layer located between the flexible membrane layer and the liquid storage layer, and through holes are respectively opened on the gasket layer vertically corresponding to the pressure buffer chamber, the lysis liquid chamber, the binding liquid chamber, the magnetic bead chamber, the front cleaning liquid chamber, the post-cleaning liquid chamber, the elution liquid chamber, the sample processing chamber, the waste liquid chamber and the loading chamber.

[0020] Furthermore, the waste liquid chamber extends upward through the top surface of the liquid storage layer and extends downward into the flow channel layer, and a waste liquid chamber piston is installed in the waste liquid chamber.

[0021] Furthermore, the sample loading chamber extends upwards through the top surface of the liquid storage layer and extends downwards through the bottom surface of the flow channel layer, and a sample loading chamber piston is installed in the sample loading chamber.

[0022] Furthermore, the nucleic acid extraction module also includes an electromagnet installed at the bottom of the sample processing chamber, the electromagnet can rotate around its own axis, and the valve core can rotate synchronously with the electromagnet.

[0023] The technical solution of the present invention has the following advantages:

[0024] 1. The universal automatic nucleic acid extraction module based on the rotating structure provided by the present invention comprises a sample processing chamber extending upwardly through the top surface of the liquid storage layer and extending downwardly into the flow channel layer, a valve core is arranged in the sample processing chamber, a sample processing chamber piston is arranged in the core cavity of the valve core, and a liquid transfer port connected to the core cavity is provided on the side wall of the core cavity; flow channels connected to the sample processing chamber are respectively provided on the top surface of the flow channel layer corresponding to the lysis liquid chamber, the binding liquid chamber, the magnetic bead chamber, the front cleaning liquid chamber, the rear cleaning liquid chamber, the elution liquid chamber, the waste liquid chamber, and the sample loading chamber, wherein the valve core can rotate around its own axis so that the liquid transfer port is connected to different flow channels, so that the sample processing chamber can be connected to different chambers by the rotation of the valve core, and different liquid flow paths can be obtained, and compared with the valves (especially microvalves) respectively arranged in the corresponding chambers, the structure is simpler, the assembly is also simpler, the cost is lower, the volume is smaller, and the weight is lighter.

[0025] 2. The universal automated nucleic acid extraction module based on the rotating structure provided by the present invention has a vortex-type flow channel designed on the inner bottom wall of the valve cavity of the valve core, which can cause the liquid flowing into the valve core to generate sufficient disturbance to suspend the magnetic beads at the bottom of the valve core, thereby ensuring that the nucleic acid is fully washed and effectively eluted, and also helps to reduce nonspecific binding, thereby ensuring the high purity of the nucleic acid extraction.

[0026] 3. In the universal automated nucleic acid extraction module based on a rotating structure provided by the present invention, the magnetic bead chamber is located between the binding liquid chamber and the sample processing chamber, and the flow channel corresponding to the binding liquid chamber extends through the flow channel corresponding to the magnetic bead chamber. In this way, when the liquid is transferred, the binding liquid will flow through the flow channel through which the magnetic beads flow, so as to further flush the flow channel corresponding to the magnetic bead chamber, thereby reducing the residue of magnetic beads in the flow channel corresponding to the magnetic bead chamber and improving the detection accuracy.

[0027] 4. In the universal automated nucleic acid extraction module based on a rotating structure provided by the present invention, a lysate chamber, a binding liquid chamber, a magnetic bead chamber, a front cleaning liquid chamber, a rear cleaning liquid chamber, and an elution liquid chamber are each equipped with a rubber plug, and a thorn-like structure is provided on the bottom wall of each of the chambers. The thorn-like structure is used to pierce the corresponding part on the flexible film layer. The rubber plug can not only push the flexible film layer toward the thorn-like structure so that the thorn-like structure pierces the flexible film layer, but also further squeeze out the liquid in each chamber to reduce the residual liquid in each chamber.

[0028] 5. The universal automated nucleic acid extraction module based on the rotating structure provided by the present invention further comprises a gasket layer located between the flexible membrane layer and the liquid storage layer, and through holes are respectively provided on the gasket layer in the vertical directions corresponding to the pressure buffer chamber, the lysis liquid chamber, the binding liquid chamber, the magnetic bead chamber, the front cleaning liquid chamber, the rear cleaning liquid chamber, the elution liquid chamber, the sample processing chamber, the waste liquid chamber and the sample loading chamber. The setting of this gasket provides more downward deformation space for the flexible membrane layer, ensuring that the thorn-like structure can pierce the flexible membrane layer.

[0029] 6. The universal automated nucleic acid extraction module based on the rotating structure provided by the present invention has a waste liquid chamber extending upward through the top surface of the liquid storage layer and extending downward into the flow channel layer. A waste liquid chamber piston is installed in the waste liquid chamber. When the waste liquid enters the waste liquid chamber, the waste liquid chamber piston is lifted up to accelerate the speed at which the waste liquid enters the waste liquid chamber from the sample processing chamber, and at the same time, the pressure in the buffer device can be buffered.

[0030] 7. The universal automated nucleic acid extraction module based on the rotating structure provided by the present invention has a sample loading chamber extending upward through the top surface of the liquid storage layer and extending downward through the bottom surface of the flow channel layer. A sample loading chamber piston is installed in the sample loading chamber. When loading the sample, the sample loading chamber piston can cause the liquid to move downstream to accelerate the loading of the sample. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] In order to more clearly illustrate the specific implementation methods of the present invention or the technical solutions in the prior art, the drawings required for use in the specific implementation methods or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are some implementation methods of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0032] Figure 1 This is a perspective schematic diagram of a three-dimensional combination of a nucleic acid providing module in an embodiment of the present invention;

[0033] Figure 2 This is a three-dimensional exploded schematic diagram of a nucleic acid providing module in an embodiment of the present invention;

[0034] Figure 3 This is a three-dimensional perspective schematic diagram of a nucleic acid providing module in an embodiment of the present invention when both the rubber plug and the piston are exposed from the top surface of the liquid storage layer;

[0035] Figure 4 A three-dimensional perspective schematic diagram of a valve core in an embodiment of the present invention;

[0036] Figure 5 This is a schematic diagram of the interior of the valve core in an embodiment of the present invention;

[0037] Figure 6 A schematic top view of a valve core in an embodiment of the present invention;

[0038] Figure 7 Schematic diagram of a three-dimensional perspective view of a flow channel layer in an embodiment of the present invention.

[0039] Description of reference numerals:

[0040] 1. Liquid storage layer; 10. Pressure buffer chamber; 100. Buffer chamber plug; 11. Lysis liquid chamber; 111. First plug; 12. Binding liquid chamber; 121. Second plug; 13. Magnetic bead chamber; 131. Third plug; 14. Front cleaning liquid chamber; 141. Fourth plug; 15. Back cleaning liquid chamber; 151. Fifth plug; 16. Eluent chamber; 161. Sixth plug; 17. Sample processing chamber; 170. Valve core; 171. Sample processing chamber piston; 172. Sealing Seal; 173, card column; 174, liquid transfer port; 175, vortex flow channel; 18, waste liquid chamber; 181, waste liquid chamber piston; 19, sample chamber; 191, sample chamber piston; 2, flexible membrane layer; 3, gasket layer; 4, flow channel layer; 40, main flow channel; 42, electromagnet; 421, card slot; 43, first flow channel; 44, second flow channel; 45, third flow channel; 46, fourth flow channel; 47, fifth flow channel; 48, waste liquid flow channel; 49, sample flow channel; A, thorn-like structure. DETAILED DESCRIPTION

[0041] The technical solution of the present invention will be described clearly and completely below in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0042] In the description of the present invention, it should be noted that the terms, "upper", "lower", "inner", "outer", etc., indicating the orientation or positional relationship are based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first", "second", "third", "fourth", "fifth", and "sixth" are used for descriptive purposes only, and cannot be understood as indicating or implying relative importance.

[0043] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0044] In addition, the technical features involved in the different embodiments of the present invention described below can be combined with each other as long as they do not conflict with each other.

[0045] like Figures 1 to 7 As shown, the present invention provides a universal automatic nucleic acid extraction module based on a rotating structure (hereinafter referred to as a nucleic acid extraction module). The nucleic acid extraction module includes a liquid storage layer 1 and a flow channel layer 4. The nucleic acid extraction module is provided with a lysate chamber 11 (also used as a sample chamber) for storing a lysate, a binding liquid chamber 12 for storing a binding liquid, a magnetic bead chamber 13 for storing magnetic beads, a pre-cleaning liquid chamber 14 for storing a cleaning liquid, a post-cleaning liquid chamber 15 for storing a cleaning liquid, an eluent chamber 16 for storing an eluent, a sample processing chamber 17, a waste liquid chamber 18 for storing waste liquid generated during the reaction process, and a sample loading chamber 19 for collecting the last nucleic acid eluent. The sample processing chamber 17 extends upward through the top surface of the liquid storage layer 1 and extends downward through the bottom wall of the flow channel layer 4. A valve core 170 is installed in the sample processing chamber 17, and a sample processing chamber piston 171 is installed in the core cavity of the valve core 170, and a liquid transfer port 174 connected to the core cavity is opened on the side wall of the core cavity. The top surface of the flow channel layer 4 is provided with flow channels connected to the sample processing chamber 17 corresponding to the lysate chamber 11, the binding liquid chamber 12, the magnetic bead chamber 13, the front cleaning liquid chamber 14, the rear cleaning liquid chamber 15, the elution liquid chamber 16, the waste liquid chamber 18, and the sample loading chamber 19. The valve core 170 can rotate around its own axis so that the liquid transfer port 174 connects different flow channels.

[0046] In the present invention, the sample processing chamber 17 extends upward through the top surface of the liquid storage layer 1 and extends downward through the bottom wall of the flow channel layer 4. A valve core 170 is installed in the sample processing chamber 17. A sample processing chamber piston 171 is installed in the core cavity of the valve core 170. A liquid transfer port 174 connected to the core cavity is provided on the side wall of the core cavity; the top surface of the flow channel layer 4 is respectively provided with flow channels (such as Figure 7 As shown), the valve core 170 can rotate around its own axis to connect the liquid transfer port 174 to different flow channels. In this way, the sample processing chamber 17 can be connected with different chambers (such as the lysis liquid chamber 11, the binding liquid chamber 12, the magnetic bead chamber 13, the front cleaning liquid chamber 14, the rear cleaning liquid chamber 15, the elution liquid chamber 16, the waste liquid chamber 18, and the loading chamber 19) through the rotation of the valve core 170, so as to obtain different liquid flow paths to complete the nucleic acid extraction. Compared with the valves (especially microvalves) respectively set in each chamber, the structure is simpler, the assembly is also simpler, the cost is lower, the volume is smaller, and the weight is lighter.

[0047] Furthermore, a sealing member 172 (such as Figure 4 As shown, the sealing member 172 is a sealing ring, the number of which is two, and of course the number may be other values), which can ensure the sealing of the sample processing chamber 17 when the valve core 170 rotates. In this embodiment, the bottom end surface of the valve core 170 is a closed end surface.

[0048] Furthermore, if Figures 4 to 6 As shown, the inner bottom wall of the valve cavity of the valve core 170 is designed with a vortex-type flow channel 175, so that the liquid flowing into the valve core 170 can be caused to generate sufficient disturbance to suspend the magnetic beads in the bottom of the valve core 170, thereby ensuring that the nucleic acid is fully washed and effectively eluted, and also helping to reduce non-specific binding, thereby ensuring the high purity of the nucleic acid extraction.

[0049] Furthermore, the height of the liquid transfer port 174 is the same as the height of the flow channel, and the bottom of the liquid transfer port 174 is substantially flush with the bottom wall of the flow channel.

[0050] Furthermore, the shape of the flow channel cross section may be semicircular but is not limited to a semicircular shape.

[0051] Furthermore, the magnetic bead chamber 13 is located between the binding liquid chamber 12 and the sample processing chamber 17, and the flow channel corresponding to the binding liquid chamber 12 and the flow channel corresponding to the magnetic bead chamber 13 are arranged in a colinear manner. In this way, when the liquid is transferred, the binding liquid will flow through the flow channel through which the magnetic beads flow, so as to further flush the flow channel corresponding to the magnetic bead chamber 13, thereby reducing the residue of magnetic beads in the flow channel corresponding to the magnetic bead chamber 13.

[0052] Furthermore, if Figure 7 As shown, the flow channels corresponding to the lysate chamber 11, the binding liquid chamber 12, the front cleaning liquid chamber 14, the rear cleaning liquid chamber 15, and the eluting liquid chamber 16 are radially extended from one end of a main channel 40, and the other end of the main channel 40 is connected to the sample processing chamber 17. For ease of understanding, the flow channels corresponding to the lysate chamber 11, the front cleaning liquid chamber 14, the rear cleaning liquid chamber 15, and the eluting liquid chamber 16 are respectively referred to as the first flow channel 43, the third flow channel 45, the fourth flow channel 46, and the fifth flow channel 47. In this embodiment, since the flow channels corresponding to the binding liquid chamber 12 and the magnetic bead chamber 13 are collinearly arranged, they are collectively referred to as the second flow channel 44, the flow channel between the sample processing chamber 17 and the waste liquid chamber 18 is referred to as the waste liquid flow channel 48, and the flow channel between the sample processing chamber 17 and the sample loading chamber 19 is referred to as the sample loading flow channel 49.

[0053] Furthermore, the lysis liquid chamber 11, the binding liquid chamber 12, the magnetic bead chamber 13, the front cleaning liquid chamber 14, the rear cleaning liquid chamber 15, and the elution liquid chamber 16 respectively extend upward through the top surface of the liquid storage layer 1, and the nucleic acid extraction module also includes a flexible membrane layer 2 arranged on the bottom wall of the liquid storage layer 1, and the flexible membrane layer 2 is used to seal the bottom ends of the lysis liquid chamber 11, the binding liquid chamber 12, the magnetic bead chamber 13, the front cleaning liquid chamber 14, the rear cleaning liquid chamber 15, and the elution liquid chamber 16 located in the liquid storage layer 1.

[0054] Further, each of the lysate chamber 11, the binding liquid chamber 12, the magnetic bead chamber 13, the front cleaning liquid chamber 14, the rear cleaning liquid chamber 15, and the eluting liquid chamber 16 is provided with a rubber plug, and each bottom wall is provided with a thorn-like structure A, which is used to pierce the corresponding part on the flexible membrane layer 2. Specifically, the diameter of the rubber plug is equal to the diameter of the corresponding chamber, so as to improve the sealing of the detection process. In this embodiment, the rubber plug is a silicone plug.

[0055] For ease of understanding, the plugs in the lysis liquid chamber 11, the binding liquid chamber 12, the magnetic bead chamber 13, the pre-cleaning liquid chamber 14, the post-cleaning liquid chamber 15, and the elution liquid chamber 16 are respectively referred to as the first rubber plug 111, the second rubber plug 121, the third rubber plug 131, the fourth rubber plug 141, the fifth rubber plug 151, and the sixth rubber plug 161.

[0056] Furthermore, a pressure buffer chamber 10 is provided in the nucleic acid extraction module, the pressure buffer chamber 10 extends upward through the top surface of the liquid storage layer 1, and extends downward through the bottom surface of the flow channel layer 4, a rubber plug is provided in the pressure buffer chamber 10 (the rubber plug in the buffer chamber is referred to as the buffer chamber rubber plug 100), and the buffer chamber rubber plug 100 can float up and down in the pressure buffer chamber 10. The pressure buffer chamber 10 is used to buffer the pressure in the downstream amplification device or detection device during the sample loading process.

[0057] Furthermore, the nucleic acid extraction module further includes a gasket layer 3 between the flexible membrane layer 2 and the liquid storage layer 1, and through holes are respectively provided on the gasket layer 3 in the vertical direction corresponding to the pressure buffer chamber 10, the lysate chamber 11, the binding liquid chamber 12, the magnetic bead chamber 13, the front cleaning liquid chamber 14, the rear cleaning liquid chamber 15, the elution liquid chamber 16, the sample processing chamber 17, the waste liquid chamber 18 and the sample loading chamber 19. In this embodiment, the provision of the gasket layer 3 provides more downward deformation space for the flexible membrane layer 2, ensuring that the thorn-like structure A can pierce the flexible membrane layer 2.

[0058] Furthermore, the waste liquid chamber 18 extends upward through the top surface of the liquid storage layer 1 and extends downward into the flow channel layer 4, and a waste liquid chamber piston 181 is installed in the waste liquid chamber 18. By pulling up the waste liquid chamber piston 181, the speed at which the waste liquid enters the waste liquid chamber 18 from the sample processing chamber 17 can be accelerated. By pulling up the waste liquid chamber piston 181, the waste liquid can be transferred into the waste liquid chamber 18, and the waste liquid chamber 18 can also serve as an air chamber to buffer the pressure inside the device during the nucleic acid extraction process.

[0059] Furthermore, the material of the flexible film layer 2 includes but is not limited to TPU, polyethylene, polypropylene, etc. The bonding method of the flexible film layer 2 and the liquid storage layer 1 includes but is not limited to double-sided adhesive, hot melting or ultrasonic welding.

[0060] Furthermore, the sample loading chamber 19 extends upward through the top surface of the liquid storage layer 1 and extends downward through the bottom surface of the flow channel layer 4, and a sample loading chamber piston 191 is installed in the sample loading chamber 19. When loading samples, the sample loading chamber piston 191 can cause the liquid to move downstream to accelerate the loading of samples.

[0061] Furthermore, the nucleic acid extraction module also includes an electromagnet 42 installed at the bottom of the sample processing chamber 17. The electromagnet 42 can rotate around its own axis, and the valve core 170 can rotate synchronously with the electromagnet 42. Specifically, a card slot 421 is provided at the top of the electromagnet 42, and a card column 173 is provided at the bottom of the valve core 170. The card column 173 is engaged with the card slot 421, so that the electromagnet 42 drives the valve core 170 to rotate synchronously. The electromagnet 42 can also absorb magnetic beads. The bottom end of the electromagnet 42 is connected to a rotating device, and the rotating device is used to drive the electromagnet 42 to rotate.

[0062] The specific operation process of the universal automated nucleic acid extraction module provided by the present invention is as follows:

[0063] The valve core 170 is rotated to connect the liquid transfer port 174 to the first flow channel 43, a syringe is passed through the first rubber plug 111, the sample is added into the lysate chamber 11, and the first rubber plug 111 is pressed down to the bottom of the lysate chamber 11, so that the thorn-like structure A pierces the flexible membrane layer 2, and the mixed liquid of the sample and the lysate enters the first flow channel 43, and at the same time, the sample processing chamber piston 171 in the sample processing chamber 17 is pulled upward to transfer the mixed liquid into the sample processing chamber 17 for lysis reaction;

[0064] After the cleavage reaction is completed, the electromagnet 42 is rotated to drive the valve core 170 to rotate, so that the liquid transfer port 174 is connected to the second flow channel 44, and the second rubber plug 121 and the third rubber plug 131 are pressed down to the bottom of the binding liquid chamber 12 and the magnetic bead chamber 13 respectively, and the sample processing chamber piston 171 is extracted at the same time, and the binding liquid and the magnetic beads are transferred to the valve core 170 for binding reaction;

[0065] During the binding reaction, the electromagnet 42 outside the bottom of the valve core 170 attracts the magnetic beads to the inner bottom surface of the valve core 170;

[0066] The electromagnet 42 is rotated to drive the valve core 170 to rotate, so that the liquid transfer port 174 is connected to the waste liquid flow channel 48, and the electromagnet 42 is stopped to press down the sample processing chamber piston 171, and at the same time, the waste liquid chamber piston 181 is lifted to transfer the waste liquid to the waste liquid chamber 18;

[0067] After the waste liquid transfer is completed, the rotating electromagnet 42 drives the valve core 170 to rotate, so that the liquid transfer port 174 is connected to the third flow channel 45, and the rotating electromagnet 42 stops;

[0068] The fourth rubber plug 141 is pressed downward, and the sample processing chamber piston 171 is extracted at the same time, so that the cleaning liquid in the pre-cleaning liquid chamber 14 is transferred to the sample processing chamber 17 for cleaning. During the cleaning process, the electromagnet 42 no longer adsorbs the magnetic beads to the inner bottom surface of the valve core 170, so that the nucleic acid is fully cleaned;

[0069] The rotating electromagnet 42 drives the valve core 170 to rotate, so that the liquid transfer port 174 is connected to the waste liquid flow channel 48, and the electromagnet 42 is activated to adsorb the magnetic beads to the inner bottom surface of the valve core 170, press down the sample processing chamber piston 171, and lift up the waste liquid chamber piston 181 to transfer the waste liquid to the waste liquid chamber 18;

[0070] After the waste liquid transfer is completed, the rotating electromagnet 42 drives the valve core 170 to rotate, so that the liquid transfer port 174 is connected to the fourth flow channel 46;

[0071] Stop rotating the electromagnet 42, press down the fifth rubber plug 151, and extract the sample processing chamber piston 171 at the same time, and transfer the cleaning liquid to the sample processing chamber 17 for cleaning;

[0072] During the cleaning process, the electromagnet 42 is disconnected and the magnetic beads are no longer adsorbed to the inner bottom surface of the valve core 170, so that the nucleic acid can be fully cleaned;

[0073] The electromagnet 42 is rotated to drive the valve core 170 to rotate, so that the liquid transfer port 174 is connected to the waste liquid flow channel 48, and the electromagnet 42 is activated to adsorb the magnetic beads to the inner bottom surface of the valve core 170;

[0074] Stop rotating the electromagnet 42, press down the sample processing chamber piston 171, and lift up the waste liquid chamber piston 181 to transfer the waste liquid to the waste liquid chamber 18;

[0075] After the waste liquid transfer is completed, the rotating electromagnet 42 drives the valve core 170 to rotate, so that the liquid transfer port 174 is connected to the fifth flow channel 47;

[0076] Stop rotating the electromagnet 42 and disconnect the electromagnet 42, so that the magnetic beads are no longer adsorbed to the inner bottom surface of the valve core 170, so that the nucleic acid can be fully eluted;

[0077] Press the sixth rubber stopper 161 downwards and extract the sample processing chamber piston 171 at the same time, transferring the eluent to the sample processing chamber 17 for elution;

[0078] After the elution is completed, the electromagnet 42 adsorbs the magnetic beads to the inner bottom surface of the valve core 170; and the electromagnet 42 is rotated to drive the valve core 170 to rotate, so that the liquid transfer port 174 is connected to the sample loading channel 49;

[0079] Stop rotating the electromagnet 42, press down the sample processing chamber piston 171, and pull up the sample loading chamber piston 191 to transfer the nucleic acid eluate to the sample loading chamber 19;

[0080] The rotating electromagnet 42 drives the valve core 170 to rotate, so that the liquid transfer port 174 is offset from the sample loading channel 49, and the sample loading chamber piston 191 is pressed to complete the sample loading process in the downstream device.

[0081] In the process of assembling the nucleic acid extraction module, the rubber plug is first installed in the corresponding chamber, and the top of the rubber plug does not expose the top surface of the liquid storage layer 1, and then the nucleic acid extraction module is inverted so that the rubber plug blocks the bottom of the corresponding chamber, and then the liquid is injected into the chamber, and each chamber is sealed with the flexible film layer 2. Compared with first placing the flexible film layer 2 at the bottom of the liquid storage layer 1, then placing the liquid storage layer 1 upright, then injecting liquid into the chamber, and finally plugging the rubber plug, this assembly process can avoid the deformation of the flexible film layer 2 caused by the insertion of the rubber plug into the chamber.

[0082] The present invention provides a universal automatic nucleic acid extraction module based on a rotating structure, which utilizes a rotating mechanism to achieve accurate transfer of liquid between different channels, thereby significantly improving the degree of automation and processing efficiency.

[0083] The present invention provides a universal automated nucleic acid extraction module based on a rotating structure. Since a vortex-type flow channel 175 is designed on the inner bottom wall of the valve cavity of the valve core 170, the liquid flowing into the valve core 170 can be prompted to produce a vortex effect and generate sufficient disturbance to suspend the magnetic beads at the bottom of the valve core 170, thereby reducing the deposition of the magnetic beads and making the distribution of the magnetic beads in the liquid more uniform, thereby ensuring that the nucleic acid is fully washed and effectively eluted, and also helping to reduce nonspecific binding, thereby ensuring the high purity of the nucleic acid extraction.

[0084] Obviously, the above embodiments are merely examples for the purpose of clear explanation, and are not intended to limit the implementation methods. For those skilled in the art, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to list all the implementation methods here. The obvious changes or modifications derived therefrom are still within the scope of protection of the invention.

Claims

1. A universal automatic nucleic acid extraction module based on a rotating structure, characterized in that: The nucleic acid extraction module comprises a liquid storage layer (1) and a flow channel layer (4) arranged in sequence from top to bottom; The nucleic acid extraction module is provided with a lysis liquid chamber (11), a binding liquid chamber (12), a magnetic bead chamber (13), a front cleaning liquid chamber (14), a rear cleaning liquid chamber (15), an elution liquid chamber (16), a sample processing chamber (17), a waste liquid chamber (18), and a sample loading chamber (19); The sample processing chamber (17) extends upward through the top surface of the liquid storage layer (1) and extends downward through the bottom wall of the flow channel layer (4); a valve core (170) is installed in the sample processing chamber (17); a sample processing chamber piston (171) is installed in the core cavity of the valve core (170); and a liquid transfer port (174) communicating with the core cavity is opened on the side wall of the core cavity; The top surface of the flow channel layer (4) is provided with flow channels connected to the sample processing chamber (17) corresponding to the lysate chamber (11), the binding liquid chamber (12), the magnetic bead chamber (13), the pre-cleaning liquid chamber (14), the post-cleaning liquid chamber (15), the elution liquid chamber (16), the waste liquid chamber (18), and the sample loading chamber (19); wherein, The valve core (170) can rotate around its own axis so that the liquid transfer port (174) can be connected to different flow channels.

2. The universal automatic nucleic acid extraction module based on a rotating structure according to claim 1, characterized in that: A vortex-shaped flow channel (175) is formed on the inner bottom wall of the core cavity of the valve core (170).

3. The universal automatic nucleic acid extraction module based on a rotating structure according to claim 1, characterized in that: The magnetic bead chamber (13) is located between the binding liquid chamber (12) and the sample processing chamber (17), and the flow channel corresponding to the binding liquid chamber (12) extends through the flow channel corresponding to the magnetic bead chamber (13).

4. The universal automatic nucleic acid extraction module based on a rotating structure according to claim 3, characterized in that: The flow channels corresponding to the lysis liquid chamber (11), the binding liquid chamber (12), the front cleaning liquid chamber (14), the rear cleaning liquid chamber (15), and the elution liquid chamber (16) extend radially from one end of a main channel (40), and the other end of the main channel (40) is connected to the sample processing chamber (17).

5. The universal automatic nucleic acid extraction module based on a rotating structure according to any one of claims 1 to 4, characterized in that: The lysing liquid chamber (11), the binding liquid chamber (12), the magnetic bead chamber (13), the front cleaning liquid chamber (14), the rear cleaning liquid chamber (15), and the elution liquid chamber (16) respectively extend upward and penetrate the top surface of the liquid storage layer (1); the nucleic acid extraction module further comprises a flexible membrane layer (2) arranged on the bottom wall of the liquid storage layer (1); the flexible membrane layer (2) is used for sealing the bottom ends of the lysing liquid chamber (11), the binding liquid chamber (12), the magnetic bead chamber (13), the front cleaning liquid chamber (14), the rear cleaning liquid chamber (15), and the elution liquid chamber (16) located in the liquid storage layer (1).

6. The universal automatic nucleic acid extraction module based on a rotating structure according to claim 5, characterized in that: The lysate chamber (11), the binding liquid chamber (12), the magnetic bead chamber (13), the pre-cleaning liquid chamber (14), the post-cleaning liquid chamber (15), and the elution liquid chamber (16) are each equipped with a rubber plug, and each bottom wall is provided with a thorn-like structure (A), and the thorn-like structure (A) is used to pierce a corresponding position on the flexible membrane layer (2).

7. The universal automatic nucleic acid extraction module based on a rotating structure according to claim 6, characterized in that: A pressure buffer chamber (10) is also provided in the nucleic acid extraction module. The pressure buffer chamber (10) extends upward through the top surface of the liquid storage layer (1) and extends downward through the bottom surface of the flow channel layer (4). A rubber plug is provided in the pressure buffer chamber (10), and the rubber plug can float up and down in the pressure buffer chamber (10).

8. The universal automatic nucleic acid extraction module based on a rotating structure according to claim 7, characterized in that: The nucleic acid extraction module further comprises a gasket layer (3) located between the flexible membrane layer (2) and the flow channel layer (4), and through holes are respectively provided on the gasket layer (3) vertically corresponding to the pressure buffer chamber (10), the lysate chamber (11), the binding liquid chamber (12), the magnetic bead chamber (13), the front cleaning liquid chamber (14), the rear cleaning liquid chamber (15), the elution liquid chamber (16), the sample processing chamber (17), the waste liquid chamber (18) and the sample loading chamber (19).

9. The universal automatic nucleic acid extraction module based on a rotating structure according to claim 8, characterized in that: The waste liquid chamber (18) extends upward through the top surface of the liquid storage layer (1) and extends downward into the flow channel layer (4). A waste liquid chamber piston (181) is installed in the waste liquid chamber (18).

10. The universal automatic nucleic acid extraction module based on a rotating structure according to claim 8, characterized in that: The sample loading chamber (19) extends upwards through the top surface of the liquid storage layer (1) and extends downwards through the bottom surface of the flow channel layer (4). A sample loading chamber piston (191) is arranged in the sample loading chamber (19).

11. The universal automatic nucleic acid extraction module based on a rotating structure according to any one of claims 1 to 4 or 6 to 10, characterized in that: The nucleic acid extraction module further comprises an electromagnet (42) installed at the bottom of the sample processing chamber (17); the electromagnet (42) can rotate around its own axis, and the valve core (170) can rotate synchronously with the electromagnet (42).

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