Culture method for microbial membrane of membrane module

Abstract

The invention relates to a culture method for a microbial membrane of a membrane module. The culture method comprises the following steps: S1, preparing a nutrient solution as a liquid culture medium; s2, a nutrient solution is poured into each membrane tube of the membrane carriers, the membrane tubes are sealed, and then the multiple membrane carriers are evenly placed in a culture pond; s3, a nutrient solution is poured into the culture pond until the liquid level just covers the top of the membrane tube, and microorganisms are put into the culture pond; s4, the temperature condition in the culture pond is maintained, microorganisms are cultured, and the microorganisms are attached to the outer side face of the membrane pipe; s5, when nutrients in the nutrient solution in the culture pond are used up, the free microorganisms in the pond body die successively, and the microorganisms attached to the membrane pipe continue to grow and breed on the membrane pipe by means of the nutrient solution in the membrane pipe to form a membrane carrier loaded with the microorganisms.

Description

Technical Field

[0001] The invention belongs to the technical field of biofilm cultivation, and in particular relates to a cultivation method for a microbial membrane of a membrane component. Background Art

[0002] In the field of water treatment, compared with activated sludge, the loss rate of immobilized microorganisms is lower, ensuring sufficient biomass. The microorganisms are loaded on a carrier with a large specific surface area, so that the microorganisms can fully contact the sewage. Immobilized microbial technology is a method of confining microbial cells in a certain spatial area to improve their efficiency and stability in wastewater treatment and environmental remediation. Immobilized microbial technology mainly includes adsorption, cross-linking and embedding. The adsorption method relies on the physical force between microorganisms and carriers to achieve immobilization. However, the organisms fixed by this method are not strong enough and are easy to fall off. The cross-linking method is to cross-link microbial cells through chemical means (using cross-linking agents such as glutaraldehyde) or physical means (changing culture conditions to promote cell self-flocculation). Chemical cross-linking may damage microbial cells, while physical cross-linking may affect cell activity. The embedding method is to trap microbial cells in the pores of water-insoluble gel polymers. Although the embedding method can better maintain cell activity, it may have problems such as low mechanical strength or greater damage to cells. Summary of the invention

[0003] In view of the above problems, the present invention provides a method for culturing a microbial membrane of a membrane module, comprising the following steps:

[0004] S1: prepare nutrient solution as liquid culture medium;

[0005] S2: perfusing nutrient solution into each membrane tube of the membrane carrier, sealing the membrane tube, and then evenly placing a number of membrane carriers in the culture pool;

[0006] S3: Pour nutrient solution into the culture tank until the liquid level just covers the top of the membrane tube, and then add microorganisms into the culture tank;

[0007] S4: maintaining the temperature conditions in the culture tank, culturing microorganisms, and attaching microorganisms to the outer side of the membrane tube;

[0008] S5: When the nutrients in the nutrient solution in the culture tank are exhausted, the free microorganisms in the tank body die one after another, and the microorganisms attached to the membrane tubes continue to grow and reproduce on the membrane tubes relying on the nutrient solution in the membrane tubes, forming a membrane carrier that carries the microorganisms.

[0009] Optionally, in step S1, the components of the nutrient solution are beef extract, peptone, and NaCl.

[0010] Optionally, in step S2, the bottom end of the membrane tube is first fixed at the corresponding membrane interface of the second spiral track to close the bottom end of the membrane tube, nutrient solution is perfused from the top end of the membrane tube, and then the top end of the membrane tube is fixed at the corresponding membrane interface of the first spiral track to close the top end of the membrane tube;

[0011] Fix the two ends of the auxiliary membrane tube in advance, inject nutrient solution into the auxiliary membrane tube through the fixed tube, and then seal the top or bottom end of the fixed tube.

[0012] Optionally, the membrane tube and the auxiliary membrane tube are made of a heavy ion microporous membrane with a pore size of 1-4 μm and a membrane thickness of 0.25 mm, and the liquid culture medium cannot flow out directly from the membrane pores.

[0013] Optionally, in step S4, the temperature of the culture tank is maintained at 25-32°C, the dissolved oxygen is 0.2-0.5 mg / L, and the pH value is 6.8-7.2.

[0014] In step S5, the growth cycle of the microorganisms includes a stable period, a growth period, a mature period and a decay period. As the nutrients in the pool are gradually consumed, in the late decay period, the free microorganisms in the culture pool die, and the remaining microorganisms are mostly attached to the membrane pores of the membrane tube. The microorganisms begin to use the nutrients in the membrane tube to grow and reproduce normally, and transmit the nutrients in the membrane tube to the outside, gradually forming a layer of colonies attached to the membrane surface around the mother body of the microorganisms in the membrane pores, completing the loading of the microorganisms.

[0015] The present invention is different from physical and chemical means, but adopts biological means, which not only solves the shortcomings of physical adsorption mode microorganisms that are not firm enough, but also solves the damage of chemical means to microbial cells. The present invention adopts a biological mosaic method (microorganisms are loaded near the membrane pores) to immobilize microorganisms, and core cells are not easy to fall off, and the biofilm automatically falls off and regenerates without manual replacement and regeneration. Because the membrane pores of the heavy ion microporous membrane are all on the surface, the fixed microbial membrane is also on the surface, and the mass transfer efficiency of the membrane material is not affected. The mechanical strength of the heavy ion microporous membrane is good, and there is no problem of fragmentation when it is subsequently applied to sewage treatment.

[0016] Optionally, the membrane carrier includes an upper fixing frame, a lower fixing frame and a plurality of membrane tubes, the upper fixing frame and the lower fixing frame are both horizontally spiral and concentrically arranged, the upper fixing frame includes a first spiral track, the starting end of the first spiral track is located at the center of the upper fixing frame, and then spirally coiled outward in a clockwise or counterclockwise direction, and the end of the first spiral track is located on the outer side of the upper fixing frame;

[0017] The lower fixing frame includes a second spiral track, the starting end of the second spiral track is located at the center of the lower fixing frame, and then spirally winds outward in the same winding direction as the first spiral track, and the end of the second spiral track is located on the outer side of the lower fixing frame;

[0018] A first transmission chain is arranged on the first spiral track, and a second transmission chain is arranged on the second spiral track; the membrane tube is arranged vertically, the top of the membrane tube is connected to the first transmission chain, and the bottom of the membrane tube is connected to the second transmission chain, so that the membrane tube can move along the two spiral tracks.

[0019] Optionally, the first spiral track has the same structure as the second spiral track, and the first transmission chain has the same structure as the second transmission chain; each side of the spiral track has a side edge, and the space between the two side edges is vacant, and the two side edges at the beginning and end of the spiral track are connected by a circular arc side edge, so that the two ends of the two side edges are closed to each other, and the two side edges form a mutually connected circular track, and the transmission chain can move in a circular motion on the spiral track along the two side edges.

[0020] Further optionally, a side edge includes two upper and lower track edges, the upper track edge and the lower track edge are on the same vertical plane, the space between the upper track edge and the lower track edge is vacant, and the transmission chain is slidably connected between the upper track edge and the lower track edge.

[0021] Further optionally, the transmission chain includes a plurality of connecting components connected end to end, the connecting components including a connecting part at the head and a connecting ring at the tail, the connecting part is solid and has at least one upper docking groove provided on the top, the upper docking groove is recessed downward, and the upper rail edge can be correspondingly inserted into the upper docking groove; at least one lower docking groove is provided at the bottom of the connecting part, the lower docking groove is recessed upward, and the lower rail edge can be correspondingly inserted into the lower docking groove; the connecting ring is hollow and is used to connect the front and rear connecting parts.

[0022] Further optionally, a plurality of rotating gears are provided in the spiral track, with a rotating gear provided at the beginning and the end of the spiral track respectively, and other rotating gears are evenly arranged along the spiral track; the rotating gears are arranged horizontally, and the convex teeth can be inserted into the connecting ring. Through the rotation of the rotating gears, the convex teeth drive the connecting ring, and then drive the transmission chain to move.

[0023] Optionally, the membrane carrier is provided with a plurality of additional membrane components, the additional membrane components comprising two fixed tubes and a plurality of horizontal auxiliary membrane tubes connected between the two fixed tubes, the fixed tubes are arranged vertically, the top is connected to the first spiral track, and the bottom is connected to the second spiral track; the plurality of auxiliary membrane tubes are arranged in sequence from top to bottom, and do not overlap or intersect with each other;

[0024] A number of vertical limiting rods are evenly arranged between the two fixed tubes to limit the position of the auxiliary membrane tube so that the auxiliary membrane tube conforms to the corresponding spiral track winding shape to form an arc-like shape to prevent the auxiliary membrane tube from touching the vertical membrane tubes on both sides. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1The figure is a schematic diagram of the structure of the membrane carrier (the rotating gear and the transmission chain are omitted for clarity);

[0026] Figure 2 is a top view schematic diagram of the upper fixing frame;

[0027] Figure 3 It is a schematic diagram of connecting the membrane tube to the first transmission chain and the second transmission chain;

[0028] Figure 4 It is a side view schematic diagram of the membrane tube connection connection part;

[0029] Figure 5 is a top view schematic diagram of an additional membrane component;

[0030] Figure 6 is a schematic side view of an additional membrane component;

[0031] Figure 7 Schematic diagram of the stirring shaft and the disturbance paddle.

[0032] In the accompanying drawings, 1-upper fixed frame, 2-lower fixed frame, 3-membrane tube, 4-first spiral track, 5-second spiral track, 6-first transmission chain, 7-side, 8-upper track edge, 9-lower track edge, 10-connecting part, 11-connecting ring, 12-upper docking groove, 13-lower docking groove, 14-membrane interface, 15-rotating gear, 16-stirring shaft, 17-disturbance paddle, 18-fixed tube, 19-auxiliary membrane tube, 20-limiting rod, 21-center line belt, 22-connecting rod, 23-main motor. DETAILED DESCRIPTION

[0033] This embodiment provides a method for culturing a microbial membrane of a membrane module, comprising the following steps:

[0034] S1: prepare nutrient solution as liquid culture medium;

[0035] S2: perfusing nutrient solution into each membrane tube of the membrane carrier, sealing the membrane tube, and then evenly placing a number of membrane carriers in the culture pool;

[0036] S3: Pour nutrient solution into the culture tank until the liquid level just covers the top of the membrane tube, and then add microorganisms into the culture tank;

[0037] S4: maintaining the temperature conditions in the culture tank, culturing microorganisms, and attaching microorganisms to the outer side of the membrane tube;

[0038] S5: When the nutrients in the nutrient solution in the culture tank are exhausted, the free microorganisms in the tank body die one after another, and the microorganisms attached to the membrane tubes continue to grow and reproduce on the membrane tubes relying on the nutrient solution in the membrane tubes, forming a membrane carrier that carries the microorganisms.

[0039] In step S1, the components of the nutrient solution are beef extract, peptone, and NaCl. 3 g of beef extract, 10 g of peptone, and 5.0 g of NaCl are dissolved in 1 L of water and used after sterilization.

[0040] In step S2, the bottom end of the membrane tube 3 is first fixed at the corresponding membrane interface of the second spiral track, so that the bottom end of the membrane tube 3 is closed, the nutrient solution is perfused from the top end of the membrane tube 3, and then the top end of the membrane tube 3 is fixed at the corresponding membrane interface of the first spiral track, so that the top end of the membrane tube is closed;

[0041] The two ends of the auxiliary membrane tube 19 are fixed in advance, the nutrient solution is poured into the auxiliary membrane tube 19 through the fixed tube 18, and then the top or bottom end of the fixed tube is sealed.

[0042] The membrane tube 3 and the auxiliary membrane tube 19 are both made of heavy ion microporous membranes with a pore size of 1-4 μm and a membrane thickness of 0.25 mm. The liquid culture medium cannot flow out directly from the membrane pores. The membrane tube of the heavy ion microporous membrane is closed and not connected to the external liquid culture medium, preventing microorganisms in the external liquid culture medium from entering the membrane tube.

[0043] In step S3 , the liquid level in the culture tank is above the top of the membrane tube 3 , and also above the uppermost auxiliary membrane tube 19 .

[0044] In step S4, the temperature of the culture tank is maintained at 25-32°C, the dissolved oxygen is 0.2-0.5 mg / L, and the pH value is 6.8-7.2.

[0045] like Figure 1-Figure 7 As shown, optionally, the membrane carrier includes an upper fixing frame 1, a lower fixing frame 2 and a plurality of membrane tubes 3, the upper fixing frame 1 and the lower fixing frame 2 are both horizontally spiral and concentrically arranged, the upper fixing frame 1 includes a first spiral track 4, the starting end of the first spiral track 4 is located at the center of the upper fixing frame 1, and then spirally coiled outward in a clockwise or counterclockwise direction, and the end of the first spiral track 4 is located on the outer side of the upper fixing frame 1;

[0046] The lower fixing frame 2 includes a second spiral track 5, the starting end of the second spiral track 5 is located at the center of the lower fixing frame 2, and then spirally winds outward in the same winding direction as the first spiral track 4, and the end of the second spiral track 5 is located on the outer side of the lower fixing frame 2;

[0047] A first transmission chain 6 is provided on the first spiral track 4, and a second transmission chain is provided on the second spiral track 5; the membrane tube is vertically arranged, the top of the membrane tube is connected to the first transmission chain 6, and the bottom of the membrane tube is connected to the second transmission chain, so that the membrane tube can move along the two spiral tracks.

[0048] Optionally, the first spiral track 4 has the same structure as the second spiral track 5, and the first transmission chain 6 has the same structure as the second transmission chain; there is a side edge 7 on each side of the spiral track, and the space between the two side edges 7 is vacant. The two side edges 7 at the beginning and the end of the spiral track are connected by a circular arc side edge, so that the two ends of the two side edges 7 are closed to each other, and the two side edges 7 form a mutually connected circulating track, and the transmission chain can move in a circular motion on the spiral track along the two side edges 7.

[0049] Further optionally, a side edge 7 includes two upper and lower track edges, the upper track edge 8 and the lower track edge 9 are on the same vertical plane, the space between the upper track edge 8 and the lower track edge 9 is vacant, and the transmission chain is slidably connected between the upper track edge 8 and the lower track edge 9.

[0050] Further optionally, the transmission chain includes a plurality of connecting components connected end to end, the connecting components including a connecting portion 10 at the head and a connecting ring 11 at the tail, the connecting portion 10 is solid and has at least one upper docking groove 12 on the top, the upper docking groove 12 is recessed downward, and the upper track edge 8 can be correspondingly inserted into the upper docking groove 12; at least one lower docking groove 13 is provided at the bottom of the connecting portion 10, the lower docking groove 13 is recessed upward, and the lower track edge 9 can be correspondingly inserted into the lower docking groove 13; the connecting ring 11 is hollow, and is used to connect the front and rear connecting portions 10.

[0051] Further optionally, a membrane interface 14 is provided on a side of the connecting portion 10 facing the outer side of the corresponding spiral track for connecting the top end or the bottom end of the membrane tube.

[0052] Further optionally, a plurality of rotating gears 15 are provided in the spiral track, with a rotating gear 15 provided at the beginning and the end of the spiral track respectively, and other rotating gears 15 are evenly arranged along the spiral track; the rotating gear 15 is arranged horizontally, and the convex teeth can be inserted into the connecting ring 11, and through the rotation of the rotating gear 15, the convex teeth drive the connecting ring 11, and then drive the transmission chain to move.

[0053] The membrane carrier of the present invention is in the form of a component, and a plurality of membrane tubes are connected between an upper fixing frame 1 and a lower fixing frame 2. Both the upper fixing frame 1 and the lower fixing frame 2 are spirally wound, so that the membrane carrier has membrane tubes 3 inside and outside, increasing the carrier area, which is conducive to increasing the amount of microorganisms. The upper and lower structures of the first spiral track 4 and the second spiral track 5 correspond to each other, and the membrane tubes 3 always remain in a vertical state when moving along the spiral track, and any adjacent membrane tubes 3 do not overlap or intersect each other.

[0054] Take the first spiral track 4 as an example. Specifically, the two sides of the first spiral track 4 are side edges 7, and the part between the two side edges 7 is vacant, which is convenient for setting the rotating gear 15. Each side edge 7 includes an upper track edge 8 and a lower track edge 9. The space between the upper track edge 8 and the lower track edge 9 is used to place the first transmission chain 6. The connecting part 10 and the connecting ring 11 of the first transmission chain 6 are alternately arranged, which can not only enable the rotating gear 15 to drive the connecting ring 11 to move, but also separate the adjacent membrane tubes 3 in space to avoid the membrane tubes 3 from being entangled with each other. All the rotating gears 15 of the first spiral track 4 rotate clockwise or counterclockwise, so that all the rotating gears 15 rotate in unison, thereby allowing the first transmission chain 6 to move cyclically along the first spiral track 4. The first transmission chain 6 is installed on the two side edges 7 on both sides. The following uses a certain connecting component on the first transmission chain 6 as a marker to introduce the movement form of the first transmission chain 6. The connecting component moves in a spiral along an inner side edge from the end to the beginning. After reaching the beginning, the connecting component moves to an outer side edge, and then moves in a spiral along the outer side edge from the beginning to the end. After reaching the end again, the connecting component moves to the inner side edge again, and continues to move in a spiral from the end to the beginning. This cyclic movement drives the first transmission chain 6 to move.

[0055] The connecting ring 11 of the first transmission chain 6 matches with the convex teeth of the rotating gear 15, and one convex tooth can be inserted into one connecting ring 11, driving the connecting ring 11 to move with the rotation of the rotating gear 15, and the next convex tooth is inserted into the next adjacent connecting ring 11, continuing to drive the first transmission chain 6 to move. The two sides of a rotating gear 15 face the two side edges respectively, and while rotating, it drives the first transmission chains 6 on the two side edges to move in opposite directions.

[0056] Driven by the first transmission chain 6, all membrane tubes 3 can move from the outside of the membrane carrier to the center of the circle, and then move from the center of the circle to the outside, repeating the cycle, so that each membrane tube can pass through various positions of the membrane carrier, and the contact conditions between the membrane tube and the nutrient solution will also change continuously, thereby increasing the bioload of all membrane tubes.

[0057] Optionally, the center of each rotating gear 15 of the first spiral track 4 is connected to the corresponding motor above the culture tank through a support rod, and the motor drives the rotating gear 15 to rotate. The rotation speed and rotation direction of all motors are the same, so that the moving speed of each part of the first transmission chain 6 is uniform.

[0058] Optionally, the positions of the rotating gear 15 on the first spiral track 4 and the rotating gear 15 on the second spiral track 5 correspond one to another; a stirring shaft 16 is connected between the two corresponding rotating gears 15, the stirring shaft 16 is vertical, and a number of disturbance paddles 17 are evenly arranged on the stirring shaft 16, the disturbance paddles 17 are horizontal or inclined, and the end of the disturbance paddle 17 away from the stirring shaft 16 does not touch the membrane tube passing outside the rotating gear 15.

[0059] The stirring shaft 16 is connected to the center of the rotating gear 15. The stirring shaft 16 can be thinner to leave more space for the disturbance paddle 17. The rotating gear 15 drives the disturbance paddle 17 to rotate through the stirring shaft 16, bringing a certain fluidity to the water area in the space between the two sides.

[0060] Optionally, the membrane carrier is provided with a plurality of additional membrane components, the additional membrane components comprising two fixed tubes 18 and a plurality of horizontal auxiliary membrane tubes 19 connected between the two fixed tubes 18, the fixed tube 18 is vertically arranged, the top is connected to the first spiral track 4, and the bottom is connected to the second spiral track 5; the plurality of auxiliary membrane tubes 19 are arranged in sequence from top to bottom, and do not overlap or intersect with each other;

[0061] A plurality of vertical limiting rods 20 are evenly arranged between the two fixed tubes 18 to limit the position of the auxiliary membrane tube 19 so that the auxiliary membrane tube 19 conforms to the corresponding spiral track winding shape to form an arc-like shape to prevent the auxiliary membrane tube 19 from touching the vertical membrane tubes on both sides.

[0062] Further optionally, a center line belt 21 is set between the two side edges 7 of the first spiral track 4, and a center line belt 21 is also set between the two side edges of the second spiral track 5. The upper and lower ends of the fixed tube 18 and the limiting rod 20 are respectively connected to the upper and lower center line belts 21 to fix the positions of the fixed tube 18 and the limiting rod 20, while improving the strength and stability of the upper fixing frame 1 and the lower fixing frame 2.

[0063] Further optionally, the fixed tube 18 is hollow, and a closable opening is provided at the top and / or bottom end of the fixed tube 18 , and the interior of the auxiliary membrane tube 19 is connected to the interior of the fixed tube 18 for inputting air or nutrient solution into the auxiliary membrane tube 19 .

[0064] Since there is a gap between the two sides of the spiral track, and the membrane tube is connected to the outer side of the connecting part 10, the spacing between the vertical membrane tubes corresponding to the two positions is larger. The present invention makes full use of this space (the space is also spiral) to set up additional membrane components, that is, to set up horizontal membrane tubes in this space to further increase the load area. The stirring shaft 16 and the disturbance paddle 17 can provide a good water flow environment for the space, and the nutrients and free microorganisms have good fluidity. In combination with the rotation of the entire membrane carrier itself, microorganisms can also attach and grow on the auxiliary membrane tube 19.

[0065] Since the auxiliary membrane tube 19 is arranged horizontally and nutrient solution is perfused in the auxiliary membrane tube 19, when there is also nutrient solution in the external environment, the drooping of the middle part of the auxiliary membrane tube 19 is not serious due to the buoyancy. If the drooping is serious, some air can be introduced into the auxiliary membrane tube 19 through the fixed tube 18 to temporarily increase its buoyancy. If the gas escapes into the external nutrient solution through the membrane hole, it can play multiple roles of disturbing the water flow, improving the oxygen content, and disturbing the upper support of the auxiliary membrane tube 19. When ventilation is needed, the top opening of the fixed tube 18 can be connected to the external air pipe.

[0066] Optionally, a plurality of connecting rods 22 are connected to the outer side of the upper fixed frame 1, and the plurality of connecting rods 22 are evenly arranged along the circumference of the upper fixed frame 1. The connecting rods 22 are vertically arranged and the bottom ends are connected to the outer side of the lower fixed frame 2. The upper parts of the connecting rods 22 extend out of the liquid surface and are connected in parallel to the rotating shaft of the main motor 23, so as to drive the entire membrane carrier to rotate through the connecting rods 22.

[0067] Since the membrane carrier is spiral-shaped, there is an opening on the outer side of the membrane carrier, the top of which corresponds to the end of the upper solid frame, and the bottom corresponds to the end of the lower fixed frame 2. The rotation direction from the center of the membrane carrier along the upper fixed frame 1 (lower fixed frame 2) toward the outside of the membrane carrier is the fixed spiral direction of the membrane carrier. By controlling the membrane carrier to rotate along its fixed spiral direction through the motor and the connecting rod 22, the nutrient solution can be continuously poured into the membrane carrier from the above opening, which is conducive to driving the nutrient solution inside the membrane carrier to flow and update, so that the microorganisms are evenly distributed.

Claims

1. A method for culturing a membrane microbial membrane, characterized in that: The following steps are involved: S1: prepare nutrient solution as liquid culture medium; S2: perfusing nutrient solution into each membrane tube of the membrane carrier, sealing the membrane tube, and then evenly placing a number of membrane carriers in the culture pool; S3: Pour nutrient solution into the culture tank until the liquid level just covers the top of the membrane tube, and then add microorganisms into the culture tank; S4: maintaining the temperature conditions in the culture tank, culturing microorganisms, and attaching microorganisms to the outer side of the membrane tube; S5: When the nutrients in the nutrient solution in the culture tank are exhausted, the free microorganisms in the tank body die one after another, and the microorganisms attached to the membrane tubes continue to grow and reproduce on the membrane tubes relying on the nutrient solution in the membrane tubes, forming a membrane carrier that carries the microorganisms.

2. The method for culturing a membrane microbial membrane according to claim 1, characterized in that: In step S1, the components of the nutrient solution are beef extract, peptone, and NaCl; In step S4, the temperature of the culture tank is maintained at 25-32°C, the dissolved oxygen is 0.2-0.5 mg / L, and the pH value is 6.8-7.

2.

3. The method for culturing a membrane microbial membrane according to claim 1, characterized in that: The membrane carrier includes an upper fixing frame, a lower fixing frame and a plurality of membrane tubes. The upper fixing frame and the lower fixing frame are both horizontally spiral and concentrically arranged. The upper fixing frame includes a first spiral track. The starting end of the first spiral track is located at the center of the upper fixing frame, and then spirally coils outward in a clockwise or counterclockwise direction. The end of the first spiral track is located on the outer side of the upper fixing frame. The lower fixing frame includes a second spiral track, the starting end of the second spiral track is located at the center of the lower fixing frame, and then spirally winds outward in the same winding direction as the first spiral track, and the end of the second spiral track is located on the outer side of the lower fixing frame; A first transmission chain is arranged on the first spiral track, and a second transmission chain is arranged on the second spiral track; the membrane tube is arranged vertically, the top of the membrane tube is connected to the first transmission chain, and the bottom of the membrane tube is connected to the second transmission chain, so that the membrane tube can move along the two spiral tracks.

4. The method for culturing a membrane microbial membrane according to claim 3, characterized in that: The first spiral track has the same structure as the second spiral track, and the first transmission chain has the same structure as the second transmission chain; each side of the spiral track has a side edge, and the space between the two side edges is vacant. The two side edges at the beginning and the end of the spiral track are connected by a circular arc side edge, so that the two ends of the two side edges are closed to each other, and the two side edges form a mutually connected circulating track, and the transmission chain can move in a circular motion on the spiral track along the two side edges.

5. The method for culturing a membrane microbial membrane according to claim 4, characterized in that: A side includes two upper and lower track sides, the upper track side and the lower track side are on the same vertical plane, the space between the upper track side and the lower track side is vacant, and the transmission chain is slidably connected between the upper track side and the lower track side.

6. The method for culturing a membrane microbial membrane according to claim 5, characterized in that: The transmission chain includes a plurality of connecting components connected end to end, and the connecting components include a connecting part at the head and a connecting ring at the tail. The connecting part is solid and has at least one upper docking groove on the top, which is recessed downward, and the upper track edge can be correspondingly inserted into the upper docking groove; at least one lower docking groove is provided at the bottom of the connecting part, which is recessed upward, and the lower track edge can be correspondingly inserted into the lower docking groove; the connecting ring is hollow and is used to connect the front and rear connecting parts.

7. The method for culturing a membrane microbial membrane according to claim 6, characterized in that: A plurality of rotating gears are arranged in the spiral track, with a rotating gear respectively arranged at the beginning and the end of the spiral track, and other rotating gears are evenly arranged along the spiral track; the rotating gears are arranged horizontally, and the convex teeth can be inserted into the connecting ring. Through the rotation of the rotating gears, the convex teeth drive the connecting ring, and then drive the transmission chain to move.

8. The method for culturing a membrane microbial membrane according to claim 3, characterized in that: The membrane carrier is provided with a plurality of additional membrane components, which include two fixed tubes and a plurality of horizontal auxiliary membrane tubes connected between the two fixed tubes. The fixed tube is vertically arranged, with the top connected to the first spiral track and the bottom connected to the second spiral track. The plurality of auxiliary membrane tubes are arranged in sequence from top to bottom, and do not overlap or intersect with each other. A number of vertical limiting rods are evenly arranged between the two fixed tubes to limit the position of the auxiliary membrane tube so that the auxiliary membrane tube conforms to the corresponding spiral track winding shape to form an arc-like shape to prevent the auxiliary membrane tube from touching the vertical membrane tubes on both sides.

9. The method for culturing a membrane microbial membrane according to claim 8, characterized in that: In step S2, the bottom end of the membrane tube is first fixed at the corresponding membrane interface of the second spiral track to close the bottom end of the membrane tube, nutrient solution is perfused from the top end of the membrane tube, and then the top end of the membrane tube is fixed at the corresponding membrane interface of the first spiral track to close the top end of the membrane tube; Fix both ends of the auxiliary membrane tube in advance, inject nutrient solution into the auxiliary membrane tube through the fixed tube, and then seal the top or bottom end of the fixed tube.

10. The method for culturing a membrane microbial membrane according to claim 8, characterized in that: The membrane tube and the auxiliary membrane tube are made of heavy ion microporous membranes with a pore size of 1-4 μm and a thickness of 0.25 mm. Liquid culture medium cannot flow out directly from the membrane pores.

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