Pipetting tip

Abstract

The pipetting suction head comprises a suction head body, the suction head body is integrated or integrally formed, the suction head body is tubular, the suction head body comprises a suction nozzle area, a quantitative liquid storage area and a pipetting gun action area from bottom to top, and an inner cavity of the suction head body is of a gradually-shrinking structure from top to bottom; wherein a sleeve assembly is arranged in the connecting action area, when the magnetic bar moves in the sleeve assembly, piston motion is formed between the sleeve assembly and the magnetic bar, and therefore liquid can be sucked into the quantitative liquid storage area through the suction nozzle area. According to the pipetting suction head, the sleeve assembly is arranged in the suction head body of the pipetting suction head, the sleeve assembly and the magnetic bar can form a piston unit, and when the magnetic bar moves in the pipetting suction head, a reagent can be directly sucked, so that a pipetting gun is replaced, the structure of nucleic acid purification equipment is simplified, and the cost of the nucleic acid purification equipment is reduced.

Description

Technical Field

[0001] The invention relates to the technical field of biological experimental equipment, in particular to a liquid transfer tip. Background Art

[0002] The automatic purification, amplification and detection all-in-one machine uses a lysis solution to lyse tissues and cells, break the cell membrane, expose a mixture of DNA, RNA and protein, and wash with a washing solution multiple times to separate the mixture of nucleic acid and protein; uses a magnetic bead solution to adsorb the separated nucleic acid multiple times, purifies the nucleic acid, and collects the purified nucleic acid; adds a PCR reaction system to perform nucleic acid amplification reaction and collect and analyze fluorescence intensity data. It is an in vitro diagnostic medical device with multiple functions.

[0003] After nucleic acid extraction and purification, a pipette is needed to transfer the purified nucleic acid to a reactor. Currently, all-in-one devices on the market use a pipette gun for liquid transfer, which not only takes up space but also increases the cost of the all-in-one device.

[0004] In view of this, the inventors of the present invention have deeply conceived and actively studied, improved and tried to develop and design the present invention in order to solve the above-mentioned problems. Summary of the invention

[0005] Based on this, it is necessary to provide a pipette tip, which can cooperate with a magnetic rod to perform piston movement to absorb liquid to achieve liquid transfer. The magnetic rod is a necessary component for nucleic acid purification. The pipette tip cooperates with the magnetic rod to perform liquid transfer, which can eliminate the pipette gun, save internal space, optimize the structure, and save equipment costs.

[0006] The present invention provides a pipette tip, comprising a pipette tip body, wherein the pipette tip body is integral or integrally formed, and the pipette tip body is tubular, and the pipette tip body comprises a nozzle area, a quantitative liquid storage area, and a connection action area from bottom to top, and the inner cavity of the pipette tip body is a gradually shrinking structure from top to bottom;

[0007] Wherein, a sleeve assembly is arranged in the connection action area. When the magnetic rod moves in the sleeve assembly, a piston movement is formed between the sleeve assembly and the magnetic rod, so that the liquid can be sucked into the quantitative liquid storage area through the suction nozzle area.

[0008] In this embodiment, a sleeve assembly is disposed in the pipette tip body, and the sleeve assembly can form a piston unit with the magnetic rod. When the magnetic rod moves in the pipette tip, the reagent can be directly absorbed, thereby replacing the pipette gun, simplifying the structure of the nucleic acid purification equipment, and reducing the cost of the nucleic acid purification equipment.

[0009] In some embodiments, the sleeve assembly includes a soft sleeve, the soft sleeve is embedded in the inner wall of the connection action area, and the soft sleeve has at least one convex ring that is interference-fitted with the magnetic rod.

[0010] In some embodiments, the sleeve assembly includes a hard sleeve and a soft sleeve wrapped around the hard sleeve; the hard sleeve is embedded in the inner wall of the connection action area, and the soft sleeve has at least one convex ring that is interference fit with the magnetic rod.

[0011] In some embodiments, three protruding rings are provided.

[0012] In some embodiments, the hard sleeve and the soft sleeve are an integral structure.

[0013] In some embodiments, the hard sleeve has an extension portion at one end close to the pipette head body, and a sealing sleeve is provided between the extension portion and the inner wall of the pipette head body.

[0014] In the nucleic acid purification system mentioned in the present invention, pipette tip holes are arranged on the multi-tube, and the pipette tips can be placed in the pipette tip holes. After the sample is loaded onto the machine, the sample is first lysed, and the magnetic rod adsorbs the magnetic beads containing the nucleic acid to be washed in the reagent holes. Then the magnetic rod can cooperate with the pipette tip to directly perform pipetting, without the need for a dedicated pipette tip, thereby saving costs and having a simple structure. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the structures shown in these drawings without paying creative work.

[0016] Figure 1 It is a structural cross-sectional view of the liquid transfer tip of the present invention;

[0017] Figure 2 It is a structural stereogram of the liquid transfer tip of the present invention;

[0018] Figure 3 It is a three-dimensional diagram of the application device of the pipette tip of the present invention.

[0019] The realization of the purpose, functional features and advantages of the present invention will be further explained in conjunction with embodiments and with reference to the accompanying drawings. DETAILED DESCRIPTION

[0020] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0021] It should be noted that all directional indications in the embodiments of the present invention (such as up, down, left, right, front, back, etc.) are only used to explain the relative position relationship, movement status, etc. between the components under a certain specific posture (as shown in the accompanying drawings). If the specific posture changes, the directional indication will also change accordingly.

[0022] In addition, the descriptions of "first", "second", etc. in the present invention are only used for descriptive purposes and cannot be understood as indicating or implying their relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include at least one of the features. In addition, "and / or" in the full text includes three solutions. Taking A and / or B as an example, it includes technical solution A, technical solution B, and technical solution that satisfies both A and B. In addition, the technical solutions between the various embodiments can be combined with each other, but it must be based on the ability of ordinary technicians in this field to implement. When the combination of technical solutions is contradictory or cannot be implemented, it should be deemed that such combination of technical solutions does not exist and is not within the scope of protection required by the present invention.

[0023] The two-hole automatic purification amplification detection integrated machine is an in vitro diagnostic medical device that integrates multiple functions by using a lysing solution to lyse tissues and cells, breaking the cell membrane, exposing a mixture of DNA, RNA and protein, and washing with a washing solution for multiple times to separate the mixture of nucleic acids and proteins; using a magnetic bead solution to adsorb the separated nucleic acids multiple times, purifying the nucleic acids, and collecting the purified nucleic acids; adding a PCR reaction system to perform a nucleic acid amplification reaction and collect and analyze fluorescence intensity data. At present, in the two-hole automatic purification amplification detection integrated machine, a pipette gun needs to be used in conjunction with a pipette tip to transfer reagents in the nucleic acid purification module, which is not only complex in structure, but also has high manufacturing costs; and in the nucleic acid purification system used in the automatic purification amplification detection integrated machine provided by the present invention, a sleeve assembly is provided in the pipette tip, and the magnetic rod used for purification can form a piston unit with the sleeve assembly, so that when the magnetic rod moves in the pipette tip, the reagent can be sucked up, thereby transferring the reagent, and the magnetic rod and the pipette tip cooperate to replace the pipette gun to directly complete the reagent transfer, which is not only more optimized and simple in structure, but also reduces the manufacturing cost of the integrated machine.

[0024] like Figure 1-Figure 2 As shown, a pipette tip is used in biochemical experiments, including a tip body, which is integral or integrally formed, and is tubular, and includes a nozzle area 30, a quantitative liquid storage area 20, and a connection action area 10 from bottom to top, and the inner cavity of the tip body is a tapered structure from top to bottom; wherein a sleeve assembly is arranged in the connection action area 10, and when the magnetic rod moves in the sleeve assembly, a piston motion is formed between the sleeve assembly and the magnetic rod, so that the liquid can be sucked into the quantitative liquid storage area 20 through the nozzle area 30.

[0025] like Figure 3 As shown, a multi-tube device is used in biochemical experiments, including a sleeve assembly hole 60 and a pipette tip body hole 61, and the sleeve assembly and the pipette tip body are respectively stored in corresponding holes and fixed together by a magnetic rod and a convex ring.

[0026] In this embodiment, a sleeve assembly is disposed in the pipette tip body, and the sleeve assembly can form a piston unit with the magnetic rod. When the magnetic rod moves in the pipette tip, the reagent can be directly absorbed, thereby replacing the pipette gun, simplifying the structure of the nucleic acid purification equipment, and reducing the cost of the nucleic acid purification equipment.

[0027] This type of pipette tip can cooperate with a magnetic rod to perform piston movement to absorb liquid to achieve liquid transfer. The magnetic rod is a necessary component for nucleic acid purification. The pipette tip and the magnetic rod can be used together to perform liquid transfer, which can eliminate the need for a pipette gun, save internal space, optimize the structure, and save equipment costs.

[0028] In some embodiments, the sleeve assembly includes a soft sleeve, the soft sleeve is embedded in the inner wall of the connection action area, and the soft sleeve has at least one convex ring that is interference-fitted with the magnetic rod.

[0029] In some embodiments, the sleeve assembly includes a hard sleeve and a soft sleeve wrapped around the hard sleeve; the hard sleeve is embedded in the inner wall of the connection action area, and the soft sleeve has at least one convex ring that is interference fit with the magnetic rod.

[0030] In some embodiments, three protruding rings are provided.

[0031] In some embodiments, the hard sleeve and the soft sleeve are an integral structure.

[0032] In some embodiments, the hard sleeve has an extension portion at one end close to the pipette head body, and a sealing sleeve is provided between the extension portion and the inner wall of the pipette head body.

[0033] In the nucleic acid purification system of the present invention, pipette tip holes are arranged on the multi-tube, and the pipette tips can be placed in the pipette tip holes. After the sample is loaded onto the machine, the sample is first lysed, and the magnetic rod adsorbs magnetic beads containing nucleic acid to be washed in the reagent chamber. Then, the magnetic rod can cooperate with the pipette tip to directly perform pipetting, without the need for a special pipette tip, thereby saving costs and having a simple structure.

[0034] The pipette tip of the present invention is industrially designed, with a range of 200 μL and a pipetting accuracy of 2 μL, and is suitable for small-volume, high-precision and high-throughput reaction systems. And through the design of the pipette action area 10, it can be compatible with the pipettes of the current mainstream high-throughput liquid workstations, without the need to redesign and build a new liquid workstation, reducing costs and being easy to promote.

[0035] The above description is only a preferred embodiment of the present invention, and does not limit the patent scope of the present invention. All equivalent structural changes made by using the contents of the present invention specification and drawings under the inventive concept of the present invention, or directly / indirectly applied in other related technical fields are included in the patent protection scope of the present invention.

Claims

1. A pipette tip, It is characterized in that The inner cavity of the pipette body is a tapered structure from top to bottom The inner cavity is provided with a sleeve assembly. When the magnetic rod moves in the sleeve assembly, a piston movement is formed between the sleeve assembly and the round rod with interference sealing fit, so that the liquid can be sucked into the liquid storage area through the suction head nozzle.

2. The pipette tip according to claim 1, It is characterized in that The sleeve assembly comprises a soft sealing ring which is embedded in the inner wall of the inner cavity and has at least one convex ring which is interference fit with the round rod.

3. The pipette tip according to claim 1, It is characterized in that The sealing ring assembly includes a hard sleeve and a sealing ring wrapped on the hard sleeve; the hard sleeve is embedded in the inner wall of the suction head through a convex ring and a groove and sealed on the inner wall of the connection action area, and the sealing ring has at least one convex ring that is interference fit with the round rod.

4. The pipette tip according to claim 2 or 3, It is characterized in that The number of the convex rings is three.

5. The pipette tip according to claim 4, It is characterized in that The hard sleeve and the soft sleeve are an integral injection-molded structure.

6. The pipette tip according to claim 5, It is characterized in that In addition to the interlocking structure, the hard sleeve is also provided with a convex ring which achieves a sealing effect through interference fit with the vertical inner wall of the suction head.

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