Method for biologically manufacturing organic silicon hydrogel through multi-enzyme cascade and application of organic silicon hydrogel

Abstract

The invention relates to the technical field of hydrogel, in particular to a method for biologically manufacturing organic silicon hydrogel through multi-enzyme cascade and application of the organic silicon hydrogel. The method comprises the following steps: S1, taking biogas slurry of fuel ethanol prepared from cassava as a raw material, mixing the biogas slurry with bacillus aryabhattai, S2, metabolizing the bacillus aryabhattai to generate amidase and unsaturated lipase, and purifying the biogas slurry; s3, performing metabolism on the bacillus aryabhattai to generate unsaturated lipase, oxygenase and carbonic anhydrase, performing parallel and cascade reaction under the cascade action of multiple enzymes, and converting inorganic silicon in the biogas slurry into organic silicon; s4, amidase, unsaturated lipase, oxygenase and carbonic anhydrase generated by metabolism of bacillus aryabhattai are subjected to parallel and series reaction under the cascade action of multiple enzymes, and linear-chain organic silicon is subjected to cyclization; and S5, performing freeze drying to obtain the organic silicon hydrogel. Compared with the prior art, the hydrogel with antibacterial, antistatic and anticorrosive properties and good biocompatibility adopts bacillus metabolic enzyme, so that the process of originally converting silicate into organic silicon at high temperature is biologically converted at normal temperature. The formed hydrogel can be used for corrosion prevention and static prevention of boilers and other equipment, has reversibility, can also be used for corrosion prevention and bacteriostasis of cosmetics, is used for sand fixation and water retention of agriculture and forestry and plant growth promotion, and can be recycled.

Description

Technical Field

[0001] The invention relates to the technical field of hydrogels, in particular to a method and application of multi-enzyme cascade biological production of organosilicon hydrogels. Background Art

[0002] As a unique "soft" material, hydrogel has the advantages of high swelling, mechanical properties, optical transparency, biodegradability, and biocompatibility, and is widely used in industry, agriculture, biomedicine, and physiological hygiene. In addition to calcium and magnesium ion scaling, boilers are also subject to sulfate corrosion and gas corrosion under the action of alkaline sulfate in the attachment layer, that is, the molten sulfate on the heating surface absorbs SO3 in the flue gas and generates composite sulfate (Na, K)(Fe, Al)(SO4)3 under the action of Fe2O3 and Al2O3 in the flue gas. Boiler corrosion is also affected by alkali metal pyrosulfate corrosion 2K3Fe(SO4)3. Patent 201610303155.1 relates to a nano-silica composite hydrogel that can be used for 3D printing and wound repair and has certain anti-corrosion properties. Publication number: CN202211151972.1 Acetophenone is reduced to phenylethanol under the action of aldehyde-keto reductase, which is completed by a one-pot method. Application (patent) number: 202210109988 discloses a method for preparing polyester polyols by a cascade biosynthesis system based on microfluidic controlled enzyme chemistry, which uses lactone as a ring-opening polymerization monomer, and norbornene-based initiator as a ring-opening polymerization initiator and ring-opening metathesis polymer, and couples biocatalytic polymerization with metal-catalyzed polymerization to a microfluidic controlled reaction platform. By regulating the two-step reaction conditions, the synthesis steps are effectively simplified, which has industrial application value (similar references include: Xu Jianhe, Yu Huilei. Construction of multi-enzyme cascade reaction and its application in the synthesis of bifunctional functional chemicals. 2023.39 (6): 2158-2189).

[0003] There are many types of hydrogels. The injectable hydrogel forms a "sol-gel" transition in situ by thermosensitive crosslinking, chemical addition crosslinking, free radical crosslinking, ionic crosslinking, photocrosslinking, and enzyme crosslinking. Enzyme-crosslinked hydrogels use natural enzymes as catalysts and do not introduce organic solvents, which can avoid the inactivation of bioactive molecules. The reaction rate of enzyme-crosslinked hydrogels is easy to control and can be effectively adjusted by adjusting the catalytic activity of the enzyme. The reaction is mild and has the characteristics of green chemistry. The hydrogel prepared by the physical crosslinking method has environmental responsiveness due to the presence of internal reversible bonds. It can adjust the release rate of the corrosion inhibitor in the loaded hydrogel and perform targeted release. It has good development prospects in the field of intelligent corrosion inhibitors.

[0004] Self-assembly refers to a technology in which basic structural units (molecules, nanomaterials, micrometer or larger scale materials) spontaneously form an ordered structure. The process of self-assembly is not a simple superposition of weak forces between a large number of atoms, ions, and molecules, but a spontaneous association between several individuals and their combination to form a tight, stable and ordered whole. The complex synergy of this whole is simple and easy to use in the self-assembly method, without the need for special equipment. It usually uses water as a solvent and can realize optical, electrical, magnetic and other functions, and even simulates biosynthesis and is highly valued. Hydrogels can be used to dissolve cellulose and silicon elements in alkaline conditions. The structure and properties of hydrogels can be characterized by liquid nuclear magnetic resonance, infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), fluorescence photovoltaics (FL) and mechanical properties tests. Although hydrogels have many superior properties, their insufficient mechanical properties limit their development in many fields. Without damaging the performance of the hydrogel, self-assembly with biofilms (biofilms) can improve the mechanical properties of the hydrogel. It can be used in boilers and other equipment for automatic descaling and recycling. This is an important research direction for hydrogels, but no specific reports have been seen yet. Summary of the invention

[0005] The present invention overcomes the deficiencies of the prior art and provides a method and application for the biomanufacturing of organosilicon hydrogel by multi-enzyme cascade.

[0006] To achieve the above purpose, a method for multi-enzyme cascade biomanufacturing of silicone hydrogel is designed, comprising the following steps: S1, using cassava biogas slurry as raw material for fuel ethanol, mixing the biogas slurry with Bacillus aeruginosa, S2, Bacillus agni metabolizes to produce amidase and unsaturated lipase to purify the biogas slurry; S3, Bacillus agnifolius metabolizes to produce unsaturated lipase, oxygenase, and carbonic anhydrase, which react in parallel and series under the cascade action of multiple enzyme species to convert inorganic silicon in biogas slurry into organic silicon; S4, amidase, unsaturated lipase, oxygenase, and carbonic anhydrase produced by the metabolism of Bacillus agnifolius, react in parallel and series under the cascade action of multiple enzyme species to cyclize the linear organosilicon; S5, freeze drying to obtain silicone hydrogel.

[0007] In the step S1, the classification name of Bacillus aryabhattai is Bacillus aryabhattai, the preservation address is China Center for Type Culture Collection, the preservation number is: CCTCC NO: M20232038, and the preservation time is October 25, 2023.

[0008] In the step S1, the biogas slurry from cassava to produce fuel ethanol contains aminopolyphenols, cassava peptides, amino acids, fulvic acid, and nutrients, including nitrogen, phosphorus, potassium, silicon, calcium, and iron; Bacillus aeruginosa is inoculated in a BL culture medium and then mixed with the biogas slurry.

[0009] In the step S2, the biogas slurry is selectively complexed with P, Ga, and Fe to precipitate and desalt under the action of amidase and unsaturated lipase at 35-50°C, 30-60 minutes, and pH 6.8-7.5 to purify the biogas slurry.

[0010] In the step S3, methionase is added for reaction, wherein the ratios of unsaturated lipase, oxygenase, methionase and carbonic anhydrase are 2-5%, 6-12%, 3-9% and 10-20% respectively; the ratio of the mixture of unsaturated lipase, oxygenase, methionase and carbonic anhydrase to the biogas slurry is 1:2000-10000.

[0011] In the step S4, the ratio of the mixture of amidase, unsaturated lipase, oxygenase and carbonic anhydrase to the biogas slurry is 1:1000-5000, wherein the addition ratios of the product obtained in step S3, amidase, unsaturated lipase, oxygenase and carbonic anhydrase are 7-15%, 5-10%, 3-9% and 15-30%, respectively.

[0012] In the step S5, the hydrogel obtained after freeze drying has a porosity of / %-85, a specific surface area of ​​302-320 mg / cm2, a density of 0.125-0.26 g / cm3, and a pore size of 15 nm-10 um.

[0013] The hydrogel can be applied to water-contacting equipment such as boilers, steam turbines, centrifuges, and biofilm self-assembly applications through the operation of pH response and control modules.

[0014] The hydrogel can be self-assembled with the biofilm through pH response and temperature response, and can be applied to cosmetics for antiseptic and antibacterial properties.

[0015] The hydrogel is used for sand fixation, water conservation and plant growth promotion in agriculture and forestry.

[0016] Compared with the prior art, the present invention has a hydrogel with good antibacterial, antistatic and anticorrosive biocompatibility. The Bacillus metabolic enzyme is used to convert silicate into organosilicon at room temperature. The formed hydrogel can be used for corrosion and antistatic of boilers and other equipment, and has reversibility. It can also be used for cosmetics antiseptic and antibacterial, for sand fixation and water conservation in agriculture and forestry, and for promoting plant growth, and can be recycled. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1The schematic diagram of ethyl anthranilate of the present invention is a component extracted from the biogas slurry of cassava for producing fuel ethanol in step S1.

[0018] Figure 2 The schematic diagram of dimethyl isooctanoate (organosilicon) of the present invention is that step S2 converts inorganic silicon in the raw material of cassava biogas slurry for producing fuel ethanol into organic silicon.

[0019] Figure 3 Schematic diagram of octamethyltetrasiloxane (organic silicon) of the present invention, which is the precursor structure of the hydrogel converted into linear cyclization in step S3.

[0020] Figure 4 Schematic diagram of octadecylnonasiloxane (organic silicon) of the present invention, which is converted into the structure of an organic silicon hydrogel through step S3. DETAILED DESCRIPTION

[0021] The present invention will be further described below with reference to the accompanying drawings. Embodiment 1

[0022] The method for producing organosilicon hydrogel by multi-enzyme cascade in this embodiment comprises the following steps: S1, using cassava biogas slurry as raw material for fuel ethanol, mixing the biogas slurry with Bacillus aeruginosa, S2, Bacillus agni metabolizes to produce amidase and unsaturated lipase to purify the biogas slurry; S3, Bacillus agnifolius metabolizes to produce unsaturated lipase, oxygenase, and carbonic anhydrase, which react in parallel and series under the cascade action of multiple enzyme species to convert inorganic silicon in biogas slurry into organic silicon, which is the anti-corrosion component of hydrogel; S4, amidase, unsaturated lipase, oxygenase, and carbonic anhydrase produced by the metabolism of Bacillus agnifolius, react in parallel and series under the cascade action of multiple enzyme species, cyclizing the linear silicone and increasing the viscosity strength of the hydrogel; S5, freeze drying to obtain silicone hydrogel.

[0023] The biogas slurry of this embodiment undergoes parallel, series and progressive reactions under the cascade action of multiple enzymes including amidase, unsaturated lipase, oxygenase, methionase and carbonic anhydrase, and can also capture CO2 in the reaction tank, thereby increasing the biological activity of the multiple enzyme cascade and the performance of recycling.

[0024] In step S1 of this embodiment, the classification name of Bacillus aryabhattai is Bacillus aryabhattai, the preservation address is China Center for Type Culture Collection, the preservation number is: CCTCC NO: M20232038, and the preservation time is October 25, 2023.

[0025] The raw materials for producing metabolic enzymes by Bacillus aeruginosa are BL culture medium, biogas slurry from cassava saccharification to produce ethanol, aminopolyphenols, cassava peptides, amino acids, and fulvic acid, with a ratio of 1:1:1:2 and a total content of 5%. The complex ions such as Ca and Fe are selectively removed. A variety of enzymes are obtained by deep fermentation of specific Bacillus, and the total enzyme activity is CFU / g. At 40°C, after 60 minutes of multi-enzyme cascade action, while the polar groups such as -OH, -COOH, and -NH2 have good hydrophilicity, the -Si-O structure is complemented to cause parallel and tandem reactions such as addition and cyclization condensation between functional groups, converting the inorganic silicon in the biogas slurry into branched and cyclized silicone hydrogels.

[0026] In step S1, the biogas slurry produced by cassava-based fuel ethanol contains aminopolyphenols, cassava peptides, amino acids, fulvic acid, and nutrients, including nitrogen, phosphorus, potassium, silicon, calcium, and iron; Bacillus aeruginosa is inoculated in a BL culture medium and then mixed with the biogas slurry.

[0027] In step S2, the biogas slurry is selectively complexed with P, Ga and Fe to precipitate and desalt under the action of amidase and unsaturated lipase at 35-50°C, 30-60min and pH 6.8-7.5 to purify the biogas slurry.

[0028] In step S3, methionase is added for reaction, wherein the ratios of unsaturated lipase, oxygenase, methionase and carbonic anhydrase are 2-5%, 6-12%, 3-9% and 10-20% respectively; the ratio of the mixture of unsaturated lipase, oxygenase, methionase and carbonic anhydrase to the biogas slurry is 1:2000-10000.

[0029] In step S4, the ratio of the mixture of amidase, unsaturated lipase, oxygenase and carbonic anhydrase to the biogas slurry is 1:1000-5000, wherein the addition ratios of the product obtained in step S3, amidase, unsaturated lipase, oxygenase and carbonic anhydrase are 7-15%, 5-10%, 3-9% and 15-30%, respectively.

[0030] In step S5, the hydrogel obtained after freeze drying has a porosity of / %-85, a specific surface area of ​​302-320 mg / cm2, a density of 0.125-0.26 g / cm3, and a pore size of 15 nm-10 um.

[0031] In specific use, the hydrogel can be applied to water-contacting equipment such as boilers, turbines, centrifuges, and biofilm self-assembly applications through pH response and control module operation.

[0032] In specific use, the hydrogel can self-assemble with the biofilm through pH response and temperature response, and be applied to cosmetics for preservation and antibacterial.

[0033] In specific usage, hydrogel is used in agriculture and forestry to fix sand and retain water and promote plant growth.

[0034] In order to verify the anti-corrosion performance of the silicone hydrogel obtained in this example, an anti-corrosion test was conducted on the silicone hydrogel: Gram-positive bacteria (Staphylococcus aureus S.aureus) and Gram-negative bacteria (Escherichia coli, E.coli) were selected to test the antibacterial properties of the samples. The bacterial concentration was 106 / (CUF / ml). After the hydrogel was added to the culture, the effective inhibition rate reached 99.0-99.9%, which was reduced to 100 times within 24 hours. After the hydrogel was added, almost no E. coli was observed, showing excellent antibacterial activity. Embodiment 2

[0035] This embodiment only describes the differences from the first embodiment, and the similarities are not described again.

[0036] The difference between this embodiment and the first embodiment is that the Bacillus aryabhattai in step S1 of this embodiment is purchased from: lyophilized product of Shanghai Xuanke Biotechnology Co., Ltd., refrigerated at 4-10°C, number: SHBCC D53239, Latin: Bacillusaryabhattai, Shanghai Collection Biotechnology Center. Embodiment 3

[0037] This embodiment is the creation of anti-corrosion, anti-static and reversible silicone hydrogel.

[0038] 1000g of cassava saccharification ethanol slurry containing aminopolyphenols, cassava peptides, amino acids, and fulvic acid was taken at a ratio of 1:1:1:2, with a total content of 5%, and Ca, Fe and other complex ions were selectively removed. Then, amidase, unsaturated lipase, oxygenase, sulfur synthase, and carbonic anhydrase obtained by deep fermentation of Bacillus sp. with the preservation number M20232036 were added to the mixture to determine the total enzyme activity CFU / g, at 40℃, after 60min of multi-enzyme cascade action, while the polar groups such as -OH, -COOH, -NH2 have good hydrophilicity, the -Si-O structure complements the functional groups to cause parallel and series reactions such as addition and cyclization condensation, converting the inorganic silicon in the biogas slurry into branched and cyclized organic silicon. By further capturing the CO2 in the reaction tank, a progressive reaction is carried out. While the covalent bonds form a three-dimensional cross-linked network, a silicone hydrogel with stable structure and good mechanical properties is formed through the temperature effect. The porosity of the hydrogel is 15nm-10um, accounting for 80%, the specific surface area / mg / cm2, the density / (g / cm3), 0.125-0.26, which is salt-sensitive and pH-responsive, and can be repeatedly frozen and condensed at -15℃ and repeatedly depolymerized at 50℃, and can be recycled. Embodiment 4

[0039] This embodiment is about organosilicon hydrogel and its application in boilers, steam turbines and centrifuges.

[0040] For example, the silicone hydrogel of Example 3 was added to a 35°C boiler cooling water system at a concentration of 0.1%. After two weeks of continuous operation, when a biofilm appeared on the outer wall of the boiler shell, it self-assembled with the hydrogel to enhance the mechanical properties. By continuing to cool down, the hydrogel together with the biofilm was precipitated and separated from the boiler cooling water system. After 50°C of temperature increase, the impurities could be separated and continued to be used. Embodiment 5

[0041] This embodiment is about the anti-corrosion performance and testing of silicone and hydrogel.

[0042] For example, the silicone hydrogel of Example 3, when used in a centrifuge, can change its own swelling degree with changes in environmental pH, temperature, etc., consistent with its network structure and organic tissue. The hydrogel controls the release and responds to slight changes in the corresponding environment (such as temperature or H value). Anticorrosive silicone hydrogels are used in the field of mechanical skin, that is, the hydrogel is coated on metal instruments such as centrifuges. The coating has a soft and smooth surface, which can greatly reduce the degree of wear. The formed biofilm can also be loaded in the hydrogel, which has the properties of reducing the corrosion degree of the centrifuge by 85% and preventing equipment scaling. Utilizing the hydrophilic and oleophobic properties of the hydrogel, the hydrogel can also selectively separate the oil and water in the centrifuge through temperature changes, and its efficiency can reach 99%, which is conducive to the recycling of silicone hydrogels.

[0043] Anti-corrosion test of silicone hydrogel: The salt concentration CaCL20.025 / ml was selected to test the antibacterial properties of the samples with Gram-positive bacteria (Staphylococcus aureus S.aureus) and Gram-negative bacteria (Escherichia coli, E.coli). The bacterial concentration was 10 6 / (CUF / ml), after adding hydrogel to the culture, it was cultured at 37℃ for 12h, and the size of the filter paper inhibition zone was observed. The effective inhibition rate reached 98.0%, which was reduced to 100 times within 24 hours. After adding hydrogel, almost no Escherichia coli was observed, showing excellent antibacterial activity. Embodiment 6

[0044] This embodiment is used to test the antistatic properties of silicone and hydrogel.

[0045] For example, the silicone hydrogel of Example 3 can be prepared by the interaction between ions and covalent bonds to form hydrogels: (1) electrostatic interaction between polyelectrolytes and multivalent ions with opposite charges to form physical hydrogels; (2) electrostatic interaction between two polyelectrolytes with opposite charges to form polyelectrolyte complex hydrogels. Its antistatic performance test: the antistatic effect prepares the proton transfer between ion cross-linked hydrogels to form hydrogels, which have responsive behavior to electric fields and electrolytes, and examines the non-covalent cross-linked network formed by physical cross-linking. The gel can be transformed into a solution by heating to form a thermoreversible hydrogel. The hydrogel polymer network structure can utilize shear thinning and electrostatic interaction. The mixed hydrogel can be stretched to 8-10 times its initial length as normal. When electrostatic interaction occurs, hydrogen bonds and host-guest interactions are used to self-repair. It is colorless, transparent, does not affect the color and transparency of the glue, and has good temperature resistance. The test shows that at -20°C, the test resistance value is 9 times, and it does not decompose at a high temperature of 320°C. It can withstand higher processing temperatures. The chemical properties are stable and will not react chemically with other additives in the glue. Good compatibility, stable product properties, good fusion, and long antistatic time. The addition amount is as low as 3%-5%, and the surface resistance can reach the 8th power, effectively controlling the production cost. It meets environmental protection requirements and has passed FDA and RoHS testing and certification. It has good dispersibility and its base adhesion strength can reach 900 J / m2. It is suitable for long-term storage in a dry state, further improving its transportability and ease of application. Embodiment 7

[0046] This embodiment is about the use of silicone and hydrogel in cosmetic preservation and in agricultural and forestry sand fixation, water conservation and plant growth promotion.

[0047] As in Example 3, the organosilicon hydrogel self-assembles with titanium dioxide in cosmetics under photocatalysis to play a skin care role, and the bacterial concentration is 10 6 / (CUF / ml), fungal concentration 10 3 / (CUF / ml); After adding 0.1% hydrogel into skin care products, there was no deterioration and no Escherichia coli and other pathogenic bacteria were observed within one year, showing excellent antibacterial activity.

[0048] The hydrogel used for sand fixation, water conservation and plant growth promotion in agriculture and forestry has the effects of Bacillus and metabolic amidase, unsaturated lipase, oxygenase, sulfhydrase and carbonic anhydrase, and acts on the biofilm of soil and plant species. The effectiveness of the hydrogel in promoting plant growth is increased by 25-40%.

[0049] The present invention uses biogas slurry from cassava fuel ethanol as raw material, and under the cascade action of multiple enzymes of Bacillus and its metabolic enzymes: amidase, unsaturated lipase, oxygenase, carbonic anhydrase, the biogas slurry is selectively purified, inorganic silicon is converted into organic silicon, and then converted into hydrogel through linear cyclization, and freeze-dried. The hydrogel used in water contact equipment such as boilers, turbines, centrifuges, etc. enhances mechanical properties and increases reversibility through self-assembly with biofilms, and can be anti-corrosive and anti-static. It can also be used for cosmetics anti-corrosion and antibacterial, and for agriculture and forestry to fix sand and retain water, and promote plant growth.

Claims

1. A method for producing organosilicon hydrogel by multi-enzyme cascade bioproduction, characterized in that: The steps include: S1, using cassava biogas slurry as raw material for fuel ethanol, mixing the biogas slurry with Bacillus aeruginosa, S2, Bacillus agni metabolizes to produce amidase and unsaturated lipase to purify the biogas slurry; S3, Bacillus agnifolius metabolizes to produce unsaturated lipase, oxygenase, and carbonic anhydrase, which react in parallel and series under the cascade action of multiple enzyme species to convert inorganic silicon in biogas slurry into organic silicon; S4, amidase, unsaturated lipase, oxygenase, and carbonic anhydrase produced by the metabolism of Bacillus agnifolius, react in parallel and series under the cascade action of multiple enzyme species to cyclize the linear organosilicon; S5, freeze drying to obtain silicone hydrogel.

2. The method for producing organosilicon hydrogel by multi-enzyme cascade according to claim 1, characterized in that: In the step S1, the classification name of Bacillus aryabhattai is Bacillus aryabhattai, the preservation address is China Center for Type Culture Collection, the preservation number is: CCTCC NO: M20232038, and the preservation time is October 25, 2023.

3. The method for producing organosilicon hydrogel by multi-enzyme cascade according to claim 1, characterized in that: In the step S1, the biogas slurry from cassava to produce fuel ethanol contains aminopolyphenols, cassava peptides, amino acids, fulvic acid, and nutrients, including nitrogen, phosphorus, potassium, silicon, calcium, and iron; Bacillus aeruginosa is inoculated in a BL culture medium and then mixed with the biogas slurry.

4. The method for producing organosilicon hydrogel by multi-enzyme cascade bioproduction according to claim 1, characterized in that: In the step S2, the biogas slurry is selectively complexed with P, Ga, and Fe to precipitate and desalt under the action of amidase and unsaturated lipase at 35-50°C, 30-60 minutes, and pH 6.8-7.5 to purify the biogas slurry.

5. The method for producing organosilicon hydrogel by multi-enzyme cascade according to claim 1, characterized in that: In the step S3, methionase is added for reaction, wherein the ratios of unsaturated lipase, oxygenase, methionase and carbonic anhydrase are 2-5%, 6-12%, 3-9% and 10-20% respectively; the ratio of the mixture of unsaturated lipase, oxygenase, methionase and carbonic anhydrase to the biogas slurry is 1:2000-10000.

6. The method for producing organosilicon hydrogel by multi-enzyme cascade according to claim 1, characterized in that: In the step S4, the ratio of the mixture of amidase, unsaturated lipase, oxygenase and carbonic anhydrase to the biogas slurry is 1:1000-5000, wherein the addition ratios of the product obtained in step S3, amidase, unsaturated lipase, oxygenase and carbonic anhydrase are 7-15%, 5-10%, 3-9% and 15-30%, respectively.

7. The method for producing organosilicon hydrogel by multi-enzyme cascade according to claim 1, characterized in that: In step S5, the hydrogel obtained after freeze drying has a porosity of / %-85 and a specific surface area of ​​302-320 mg / cm 2 , density is 0.125-0.26g / cm 3 , pore size is 15nm-10um.

8. The use of multi-enzyme cascade biomanufacturing of organosilicon hydrogel according to claim 1, characterized in that: The hydrogel can be applied to water-contacting equipment such as boilers, steam turbines, centrifuges, and biofilm self-assembly applications through the operation of pH response and control modules.

9. The use of multi-enzyme cascade biomanufacturing of organosilicon hydrogel according to claim 1, characterized in that: The hydrogel can be self-assembled with the biofilm through pH response and temperature response, and can be applied to cosmetics for antiseptic and antibacterial properties.

10. The use of multi-enzyme cascade biomanufacturing of organosilicon hydrogel according to claim 1, characterized in that: The hydrogel is used for sand fixation, water conservation and plant growth promotion in agriculture and forestry.

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